본 발명은 상기한 문제점을 해결하고, 상기의 필요성에 의하여 안출된 것으로서, 본 발명의 주된 목적은 IgA 신증 및 TGBM 신증 질환의 조기진단 및 진행정도를 효과적으로 진단할 수 있는 새로운 바이오마커 단백질 또는 그 면역원성 단편을 제공하는 데 있다. 본 발명의 다른 목적은 상기 바이오마커 단백질에 대한 항체 및 이를 이용한 조성물 및 진단키트를 제공하는데 있다. The present invention solves the above problems, and the present invention has been made by the necessity of the above, the main object of the present invention is a new biomarker protein or its immunity that can effectively diagnose the early diagnosis and progression of IgA nephropathy and TGBM nephropathy disease To provide the original fragments. Another object of the present invention is to provide an antibody against the biomarker protein, and a composition and diagnostic kit using the same.
본 발명의 목적을 달성하기 위하여, 본 발명은 정상인의 뇨와 비교하여 신증 개체의 뇨에서 보다 증감하는 하기 군에서 선택된 단백질을 유효성분으로 포함하는 IgA 신증과 TGBM 신증 질환의 진단용 바이오마커를 제공한다. 진단 바이오마커 단백질 발굴의 위하여 label-free 정량법을 이용하였다. 분석의 편의성과 재현성을 위하여 IgA 신증 환자그룹(이하, “IgAN 그룹”이라 칭함), TGBM 환자그룹(이하, “TGBM 그룹”이라 칭함)을 모두 10대에 국한하여 실험을 진행하였고, 뇨 전체의 단백질을 분석하지 않고 뇨 내의 엑소좀만을 분리하여 실험을 진행 하였다. In order to achieve the object of the present invention, the present invention provides a biomarker for diagnosing IgA nephropathy and TGBM nephropathy disease comprising a protein selected from the following group to increase or decrease in the urine of nephrotic individuals as compared to the urine of normal individuals . Label-free quantitation was used to identify diagnostic biomarker proteins. For the convenience and reproducibility of the analysis, the IgA nephropathy group (hereinafter referred to as the "IgAN group") and the TGBM patient group (hereinafter referred to as the "TGBM group") were all limited to 10 generations. The experiment was carried out by separating only exosomes in urine without analyzing proteins.
본 발명에서 개체(subject)란 IgA 신증 또는 TGBM 신증 환자이며, 면역원성 단편이란 본 발명의 바이오 마커 단백질에 대한 항체에 의해 인식될 수 있는 하나 이상의 에피토프(epitope)를 가지고 있는 바이오마커 단백질의 단편을 의미한다.In the present invention, a subject is a patient with IgA nephropathy or TGBM nephropathy, and an immunogenic fragment is a fragment of a biomarker protein having one or more epitopes that can be recognized by an antibody against a biomarker protein of the present invention. it means.
본 발명의 다른 목적을 달성하기 위해, 본 발명은 본 발명의 바이오마커 단백질 또는 그 면역원성 단편에 특이적으로 결합하는 항체를 유효성분으로 포함하는 IgA 신증 및/또는 TGBM 신증 질환의 진단제를 제공한다. 본 발명의 항체는 폴리클로날 항체일 수도 있으나, 모노클로날 항체인 것이 바람직하다. In order to achieve another object of the present invention, the present invention provides a diagnostic agent for IgA nephropathy and / or TGBM nephropathy disease comprising an antibody that specifically binds to the biomarker protein or immunogenic fragment thereof of the present invention as an active ingredient. do. The antibody of the present invention may be a polyclonal antibody, but is preferably a monoclonal antibody.
폴리클로날 항체는 당업자에 알려진 종래방법에 따라 면역원인 바이오마커 단백질 또는 그 단편을 외부숙주에 주사함으로써 제조될 수 있다. 외부숙주는 마우스, 래트, 양, 토끼와 같은 포유동물을 포함한다. 면역원은 근내, 복강내 또는 피 하주사방법으로 주사되며, 일반적으로 항원성을 증가시키기 위한 보조제(adjuvant)와 함께 투여된다. 외부숙주로부터 정기적으로 혈액을 채취하여 향상된 역가 및 항원에 대한 특이성을 보이는 혈청을 수거하거나 이로부터 항체를 분리 정제한다. Polyclonal antibodies can be prepared by injecting a biomarker protein or fragment thereof as an immunogen into an external host according to conventional methods known to those skilled in the art. Outer hosts include mammals such as mice, rats, sheep and rabbits. Immunogens are injected intramuscularly, intraperitoneally or subcutaneously, and are usually administered with an adjuvant to increase antigenicity. Blood is collected periodically from the external host to collect serum that shows improved titers and specificity for the antigen or to separate and purify the antibody therefrom.
모노클로날 항체는 당업자에 알려진 융합에 의한 불멸화된 세포주 생성기술[Koeher and Milstein 1975, Nature, 256:495)]에 의해 제조될 수 있다. 그 제조방법을 간단히 설명하면 다음과 같다. 먼저 순수한 단백질을 20 ㎍을 얻어서 Balb/C 쥐에 면역화를 시키거나 펩타이드를 합성하여 소혈청 알부민과 결합시켜 쥐에 면역화 시킨다. 그 후에 쥐에서 분리된 항원-생산 임파구를 인간 또는 마우스의 미엘로마와 융합하여 불멸화된 하이브리도마를 생성하며, 엘라이져(ELISA)방법을 사용하여 원하는 모노클노날 항체를 생성하는 하이브리도마 세포만을 선택하여 증식한 후 배양물로부터 모노클로날 항체를 분리 정제한다. Monoclonal antibodies can be prepared by techniques of generation of immortalized cell lines by fusion known to those skilled in the art (Koeher and Milstein 1975, Nature, 256: 495). The manufacturing method is briefly described as follows. First, 20 μg of pure protein is immunized to Balb / C mice or a peptide is synthesized and combined with bovine serum albumin to immunize mice. Thereafter, antigen-producing lymphocytes isolated from mice are fused with myeloma in humans or mice to produce immortalized hybridomas, and only hybridoma cells that produce the desired monoclonal antibody using ELISA method. After selection and propagation, monoclonal antibodies are isolated and purified from the culture.
본 발명의 다른 목적을 달성하기 위해, 본 발명은 개체의 체액에서 본 발명의 바이오마커 단백질 또는 그 면역원성 단편의 존재를 검출하는 단계를 포함하는, 개체의 당뇨병성 신증 진행 정도에 대한 진단 방법을 제공한다. 본 발명의 진단 방법에서, 인간 혈액인 것을 특징으로 하며, 상기 검출 단계는 개체의 간의 혈액 용액으로부터 2차원(2-D) 전기영동으로 바이오마커 단백질 또는 그 면역원성 단편의 존재를 직접 검출하거나, 혈액을 본 발명의 항체와 접촉시켜 항원항체반응을 통해 바이오마커 단백질 또는 그 면역원성 단편의 존재를 간접적으로 확인하는 것을 포함한다.To achieve another object of the present invention, the present invention provides a method for diagnosing the progression of diabetic nephropathy in a subject, comprising detecting the presence of the biomarker protein or immunogenic fragment thereof of the subject in body fluids of the subject. to provide. In the diagnostic method of the present invention, it is characterized in that the human blood, wherein the detecting step directly detects the presence of the biomarker protein or immunogenic fragment thereof by two-dimensional (2-D) electrophoresis from the blood solution of the liver of the individual, Contacting blood with an antibody of the invention to indirectly confirm the presence of a biomarker protein or immunogenic fragment thereof through an antigenic antibody reaction.
항원항체반응으로서 현재 널리 알려진 immunoassay 법은 효소면역측정 법(ELISA, Coated tube), 항체결합 magnetic particle을 tube에 결합시킨 다음 antigen-tracer와 난분해성 오염물질을 서로 경쟁적으로 반응시켜 효소반응을 유발시켜 정량하는 magnetic particle법, 항체결합 latex particle을 이용한 latex particle법 등이 있다. 본 발명의 다른 목적을 달성하기 위해, 본 발명은 본 발명의 바이오 마커 단백질 또는 그 면역원성 단편에 특이적으로 결합하는 항체를 유효성분으로 포함하는 당뇨병성 신증의 진단 키트를 제공한다. Currently known immunoassay as antigen antibody reaction, enzyme immunoassay (ELISA, Coated tube), antibody-bound magnetic particles are bound to the tube, and then antigen-tracer and refractory contaminants react competitively to induce enzyme reaction. Magnetic particle method to quantify, latex particle method using antibody-bound latex particles and the like. In order to achieve another object of the present invention, the present invention provides a diagnostic kit for diabetic nephropathy comprising an antibody that specifically binds to the biomarker protein of the present invention or an immunogenic fragment thereof.
본 발명의 진단키트는 당업자에 알려진 종래의 제조방법에 의해 제조되며, 전형적으로 동결건조형태의 항체와 버퍼, 안정화제, 불활성 단백질 등을 포함한다. 상기 항체는 방사종, 형광원, 효소 등에 의해 표지화 될 수 있다. 본 발명의 단일클론항체는 immunoassay 키트(ELISA, antibody coated tube test, lateral-flow test, potable biosensor)에 다양하게 이용될 수 있을 뿐만 아니라, 보다 높은 특이도와 민감도를 나타내는 항체의 개발을 통한 다양한 당뇨병성 합병증 검출 스펙트럼을 갖는 단백질칩 개발에도 이용될 수 있다.Diagnostic kits of the present invention are prepared by conventional methods known to those skilled in the art, and typically include lyophilized antibodies, buffers, stabilizers, inactive proteins and the like. The antibody may be labeled by radioactive species, fluorescent sources, enzymes, and the like. Monoclonal antibodies of the present invention can be used in a variety of immunoassay kits (ELISA, antibody coated tube test, lateral-flow test, potable biosensor), as well as various diabetic through the development of antibodies showing higher specificity and sensitivity It can also be used to develop protein chips with a complication detection spectrum.
본 발명의 다른 목적을 달성하기 위해, 본 발명은 IgA 신증 또는 TGBM 신증 환자의 뇨에 증감하는 군에서 선택된 단백질을 유효성분으로 포함하는 IgA 신증 또는 TGBM 신증 질환의 치료 및 예방용 조성물을 제공한다. In order to achieve another object of the present invention, the present invention provides a composition for the treatment and prevention of IgA nephropathy or TGBM nephropathy disease comprising a protein selected from the group of increasing or decreasing the urine of patients with IgA nephropathy or TGBM nephropathy.
본 발명의 약학적 조성물은 약제학적 분야에서 공지된 방법에 의해 약학적으로 허용되는 담체, 부형제, 희석제 등과 혼합하여 주사제 등의 제형으로 제조되어 정맥주사 등으로 투여될 수 있다. 본 발명에 따른 약학적 조성물의 투여량은 환자의 연령, 성별, 상태, 질환의 증상에 따라 적절히 선택될 수 있으며, 바람직하게는 성인기준으로 1일 0.001~100 ㎎의 단백질을 투여할 수 있다.The pharmaceutical composition of the present invention may be mixed with a pharmaceutically acceptable carrier, excipient, diluent, etc. by a method known in the pharmaceutical art, prepared in the form of an injection, etc., and administered by intravenous injection. The dosage of the pharmaceutical composition according to the present invention may be appropriately selected according to the age, sex, condition, and symptoms of the disease of the patient, and preferably, 0.001 to 100 mg of protein per day may be administered on an adult basis.
본 발명은 IgA 신증 또는 TGBM 신증 질환을 뇨로부터 쉽게 진단할 수 있는 기술이다. 현재까지 IgA 신증이나 TGBM 신증 환자의 진단에 대한 상업적으로 판매되는 효율적인 진단제는 없다. 본 발명의 바이오마커 단백질 조성물은 IgA 신증과 TGBM 신증 환자의 뇨에서 간단히 측정할 수 있으며, 조기 진단 및 진행 정도를 미리 예견하고 파악할 수 있다는 점에서 매우 유용하다. The present invention is a technique that can easily diagnose IgA nephropathy or TGBM nephropathy disease from urine. To date, there are no commercially available efficient diagnostic agents for the diagnosis of patients with IgA nephropathy or TGBM nephropathy. The biomarker protein composition of the present invention can be easily measured in the urine of patients with IgA nephropathy and TGBM nephropathy, and is very useful in that it can predict and understand early diagnosis and progression in advance.
또한, 본 발명의 바이오마커 단백질을 기초로 제조된 단일클론항체는 IgA 신증과 TGBM 신증 질환의 조기진단 및 진행 정도를 진단할 수 있는 immunoassay 키트(ELISA, antibody coated tube test, lateral-flow test, potable biosensor)에 이용될 수 있을 뿐만 아니라, 보다 높은 특이도와 민감도를 나타내는 항체의 개발을 통한 다양한 사구체신염 중에서 IgA 신증과 TGBM 신증의 조기진단 및 진행정도의 검출 스펙트럼을 갖는 단백질 칩 개발에 이용될 수도 있다. In addition, the monoclonal antibody prepared based on the biomarker protein of the present invention is an immunoassay kit (ELISA, antibody coated tube test, lateral-flow test, potable) for diagnosing the early diagnosis and progression of IgA nephropathy and TGBM nephropathy disease. biosensor), as well as protein chips with early detection and progression detection of IgA nephropathy and TGBM nephropathy among various glomerulonephritis through the development of antibodies with higher specificity and sensitivity. .
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하기로 한다. 이들 실시예는 단지 본 발명을 예시하기 위한 것이므로, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는다.Hereinafter, the present invention will be described in more detail with reference to Examples. Since these examples are only for illustrating the present invention, the scope of the present invention is not to be construed as being limited by these examples.
<실시예 1> 뇨 샘플 준비(Urinary sample preparation)Example 1 Urinary sample preparation
전체 환자와 정상인의 뇨는 경북대학교 병원에서 제공되었다. 2006년 7월부터 2008년 4월까지 수집된 뇨 중에서 10대의 신장 조직검사를 확인된 5명의 IgA 신 증 환자, 7명의 TGBM환자 및 12명의 정상인의 뇨를 선택했다. 모든 제공자의 아침 첫 뇨를 채취하여 protease inhibitor(1.67 ㎖ of 100 mM NaN3/2.5 ㎖ of 10 mM PMSF/50㎕ of 1 mM Leupeptin)를 처리하여 -80℃에 보관한다. 뇨 시료는 뇨 크레아티닌 수치로 표준화 했다. 표준화된 뇨 시료[IgA 신증 환자(IgAN): 88.1㎖, TGBM 환자(thinGBM): 65.2 ㎖]를 사용하여 녹는 즉시 강하게 볼텍스 하여 사용하였으며 사용된 뇨의 임상병리학적 정보는 표1 에 나타내었다.Urine of all patients and normal people was provided by Kyungpook National University Hospital. Among the urine collected from July 2006 to April 2008, we selected five patients with IgA nephropathy, seven patients with TGBM, and 12 healthy people who had confirmed 10 renal biopsies. First urine from all donors is collected and stored at -80 ° C with protease inhibitors (1.67 ml of 100 mM NaN 3 /2.5 ml of 10 mM PMSF / 50 µl of 1 mM Leupeptin). Urine samples were normalized to urine creatinine levels. Standardized urine samples (IgA nephropathy (IgAN): 88.1 mL, TGBM patients (thinGBM): 65.2 mL) were used as strong vortex immediately upon melting and the clinicopathological information of the urine used is shown in Table 1.
표1. 실험에 사용된 뇨의 임상병리학적 정보Table 1. Clinical Pathologic Information of Urine Used in Experiments
sex sex
age age
Urine protein (/dl)Urine protein (/ dl)
Ucr (/dl)Ucr (/ dl)
Histological classificationHistological classification
IgA 신증 IgA Nephropathy
MM
1919
133133
3423.03423.0
Haas subclassIIHaas subclassII
FF
1818
80.580.5
1812.31812.3
Haas subclassIIHaas subclassII
FF
1616
83.383.3
3891.33891.3
Haas subclassIIHaas subclassII
MM
1919
31.831.8
4242.04242.0
Haas subclassIIHaas subclassII
MM
1919
30.430.4
3513.33513.3
Haas subclassIHaas subclassI
TGBM 신증 TGBM Nephropathy
MM
1919
40.940.9
5758.25758.2
TGBMTGBM
MM
1919
32.332.3
3916.53916.5
TGBMTGBM
MM
1919
30.030.0
4512.94512.9
TGBMTGBM
MM
1919
43.643.6
5680.55680.5
TGBMTGBM
MM
1818
34.534.5
3528.03528.0
TGBMTGBM
MM
1818
28.828.8
2509.52509.5
TGBMTGBM
MM
1919
31.031.0
5817.05817.0
TGBMTGBM
<실시예 2> 엑소좀 정제(Purification of exosome)Example 2 Purification of exosomes
2-1. 엑소좀 정제 2-1. Exosome tablets
뇨 시료는 각각의 뇨 크레아티닌 수치를 체액분석기를 통해 확인하고 동량의 크레아티닌이 포함될 수 있게 부피를 정한후 세 군의 뇨를 합친다. 합쳐진 뇨 시료를 1차 원심분리(17,000 × g for 15 min at 4 ℃)를 통해 전체 세포나 큰 막단백질 등의 파편을 제거한다. 1차 원심분리의 상등액을 2차 원심분리(200,000×g for 1 h at 4 ℃)를 실시하여 저밀도 막 소포단백질을 침전시킨다. 침전된 결과물은 분 리 버퍼(250 mM sucrose/10 mM triethanolamine/0.5 mM PMSF/1 μM leupeptin) 50 ㎕를 이용하여 재현탁시키고 1.5 ㎖ 폴리프로필렌 튜브에 옮기고 60 ㎎/㎖ DTT를 첨가하여 60 ℃ 에서 10 분간 반응시켜 THP를 분리한다. 그 후 분리 버퍼를 첨가하여 원심분리(200,000 × g for 1 h at 4 ℃)를 실시 저밀도 막 소포단백질을 침전 및 분리한다.The urine sample checks each urine creatinine level through a bodily fluid analyzer, sets the volume to contain the same amount of creatinine, and combines the three groups of urine. The combined urine samples are subjected to primary centrifugation (17,000 × g for 15 min at 4 ° C) to remove debris, such as whole cells or large membrane proteins. The supernatant of the first centrifugation is subjected to a second centrifugation (200,000 × g for 1 h at 4 ° C.) to precipitate the low density membrane vesicle protein. The precipitated product was resuspended using 50 μl of separation buffer (250 mM sucrose / 10 mM triethanolamine / 0.5 mM PMSF / 1 μM leupeptin), transferred to a 1.5 ml polypropylene tube and added 60 mg / ml DTT at 60 ° C. The reaction is separated for 10 minutes to separate THP. After that, centrifugation (200,000 × g for 1 h at 4 ° C.) is performed by adding a separation buffer to precipitate and separate the low density membrane vesicle protein.
2-2. 엑소좀 순도 확인(Confirmation of exosome purity)2-2. Confirmation of exosome purity
분리된 엑소좀의 순도를 확인하기위해 다음 세가지 분획을 얻었다. ① 뇨 침전물 (10 ㎖의 뇨를 1차 원심분리 17.000×g 15분하여 얻어진 침전물); ②뇨 엑소좀(10 ㎖의 뇨를 1차 원심분리 17,000×g 15분의 상등액을 2차 원심분리 200,000×g 1 시간 하여 얻어진 침전물) ③10 ㎖의 1차 원심분리 상등액을 동량의 아세톤을 첨가하여 침전시킨 단백질. 얻어진 분획을 12 % 아크릴아마이드 젤에 로딩하여 전기영동 하였다. 젤의 단백질을 니트로셀룰로스 막에 옮기고 웨스턴 블랏을 실시하였다. 상온에서 한 시간동안 TBS-T(20 mM Tris, 500 mM NaCl, 0.05 % Tween-20)에 녹인 5 % 탈지유에서 블락킹 한 후 엑소좀의 표지 단백질인 anti-AQP2(aquapolin 2) (Santa Cruz, CA, USA) 와 anti-NHE3(Na/H exchanger 3) (Chemicon, Temecula, CA, USA) 1차항체를 포함한 5 % 탈지유/TBS-T용액을 넣어 4 ℃에서 16 시간 반응시킨다. 그 후 horseradish peroxidase(Santa Cruz, CA, USA)와 결합된 2차 항체 secondary를 붙이고 chemiluminescence(ECL, Amersham Pharmacia Biotech)감지 방법을 이용하여 이미지를 얻는다. 엑소좀의 분리 순도를 확인하기 위하여 엑소좀 표지 단백질인 두가지 단백질(NHE3 and AQP2) 항체를 이용하여 ①뇨 침전물 (10 ㎖의 뇨를 1차 원심분리 17.000×g 15분하여 얻어진 침전물); ②뇨 엑소좀 (10 ㎖의 뇨를 1차 원심분리 17,000×g 15분의 상등액을 2차 원심분리 200,000×g 1시간 하여 얻어진 침전물) ③10 ㎖의 1차 원심분리 상등액을 동량의 아세톤을 첨가하여 침전시킨 단백질의 세가지 분획으로 웨스턴 블랏을 실시하였으며 도 1{(A) Rabbit anti-Na+/H+ exchanger-3 [NHE-3] polyclonal antibody (B) Mouse anti-Aquaporin-2 [AQP-2] monoclonal antibody. (1) urinary sediment(pellet after 17,000 × g 15 min spin); (2) urinary exosome(pellet after 200,000 × g 1hr spin from supernatant of 17,000 × g 15 min spin) (3) a 10㎖ aliquot of 17,000 × g supernatant was used to isolate urinary protein by acetone precipitation.}에 나타내었다. 2번 분획에서는 엑소좀의 마커인 두 단백질의 발현을 볼 수 있으나 나머지 두 분획에서는 확인되지 않았다. The following three fractions were obtained to confirm the purity of the separated exosomes. ① urine precipitate (a precipitate obtained by first centrifuging 17.000 × g 15 minutes of 10 ml of urine); ② Urine exosomes (a precipitate obtained by first centrifuging 17,000 × g 15 minutes of supernatant for 10 minutes of urine and 200,000 × g for 1 hour of secondary centrifugation) ③ Add 10 ml of primary centrifuge supernatant to the same amount of acetone Precipitated Protein. The obtained fractions were loaded on 12% acrylamide gel and electrophoresed. The protein of the gel was transferred to nitrocellulose membrane and subjected to western blot. After blocking at 5% skim milk dissolved in TBS-T (20 mM Tris, 500 mM NaCl, 0.05% Tween-20) for 1 hour at room temperature, anti-AQP2 (aquapolin 2), a marker protein of exosomes (Santa Cruz, CA, USA) and anti-NHE3 (Na / H exchanger 3) (Chemicon, Temecula, CA, USA) were added 5% skim milk / TBS-T solution containing the primary antibody and reacted for 16 hours at 4 ℃. After that, a secondary antibody secondary bound to horseradish peroxidase (Santa Cruz, CA, USA) was attached and the image was obtained using chemiluminescence (ECL, Amersham Pharmacia Biotech) detection method. In order to confirm the isolation purity of the exosomes, two urine proteins (NHE3 and AQP2), which are exosome-labeled proteins, were used: (1) urine sediment (a precipitate obtained by first centrifuging 17.000 × g 15 minutes of 10 ml urine); ② Urine exosomes (a precipitate obtained by first centrifuging 17,000 × g 15 minutes of supernatant for 10 minutes of urine and 200,000 × g for 1 hour of secondary centrifugation) ③ Add 10 ml of primary centrifuge supernatant to the same amount of acetone Western blot was performed with three fractions of precipitated protein. FIG. 1 (A) Rabbit anti-Na + / H + exchanger-3 [NHE-3] polyclonal antibody (B) Mouse anti-Aquaporin-2 [AQP-2] monoclonal antibody . (1) urinary sediment (pellet after 17,000 × g 15 min spin); (2) urinary exosomes (pellet after 200,000 × g 1hr spin from supernatant of 17,000 × g 15 min spin) (3) a 10 ml aliquot of 17,000 × g supernatant was used to isolate urinary protein by acetone precipitation. In the second fraction, expression of two proteins, markers of exosomes, was seen, but not in the other two fractions.
<실시예 3> 효소 소화(Enzyme digestion) Example 3 Enzyme digestion
얻어진 엑소좀 침전물을 14 ㎕ 0.3 % SDS용액에 현탁시킨 후 5 ㎕의 아크릴아마이드용액 (30%) 0.7 ㎕ of 1 % ammonium persulfate, 와 0.3 ㎕ of TEMED를 섞어 준 후 약 30 분 실온에서 중합시킨다. 작은 젤을 수술용 칼을 이용하여 1 mm3 크기로 자른 후 25 mM ABC(pH 8.0) 버퍼와 25 mM ABC(pH 8.0)/50% ACN 버퍼를 번갈아서 20 분씩 씻어준다. 그 후 감압건조 이용하여 젤을 말리고 일반적인 인-젤 다이제스천 방법을 따른다. 각 단백질은 10 mM DTT를 첨가하여 56 °C에서 30 분간 환원시키고 55 mM iodoacetamide(IAA)를 첨가하여 실온 암반응하여 알킬화한다. 다시 젤 조각을 25 mM ABC (pH 8.0) 버퍼와 25 mM ABC(pH 8.0)/50% ACN버퍼를 번갈아서 20 분씩 씻어주고 감압건조 이용하여 젤을 말린다. 25 mM ABC 버퍼에 100 ng/㎕l 농도가 되게 트립신을 녹여 젤 조각과 함께 37 °C 16 시간동안 반응시킨다. 트립신에 의해 잘린 펩타이드를 25 mM ABC, 5 % formic acid/25 mM ABC/50% ACN in water 용액을 이용하여 세 번 추출해내고 각 추출된 펩타이드 용액은 감압건조를 이용하여 말리고 고상 단백질 추출법에 의해 펩타이드 만을 모은다.The obtained exosome precipitate is suspended in 14 μl 0.3% SDS solution, and 5 μl of acrylamide solution (30%) is mixed with 0.7 μl of 1% ammonium persulfate and 0.3 μl of TEMED, and then polymerized at room temperature for about 30 minutes. Cut a small gel into a 1 mm 3 size using a surgical knife, and then rinse with alternating 25 mM ABC (pH 8.0) buffer and 25 mM ABC (pH 8.0) / 50% ACN buffer for 20 minutes. The gel is then dried using reduced pressure drying followed by the usual in-gel digest cloth method. Each protein was reduced for 30 minutes at 56 ° C by the addition of 10 mM DTT and alkylated by room dark reaction by the addition of 55 mM iodoacetamide (IAA). Again, wash the gel pieces alternately in 25 mM ABC (pH 8.0) buffer and 25 mM ABC (pH 8.0) / 50% ACN buffer for 20 minutes and dried under reduced pressure to dry the gel. Trypsin is dissolved in 25 mM ABC buffer to a concentration of 100 ng / μl and reacted with the gel pieces at 37 ° C for 16 hours. Trypsin-cut peptides were extracted three times using 25 mM ABC, 5% formic acid / 25 mM ABC / 50% ACN in water solution, and each extracted peptide solution was dried using reduced pressure drying and the peptide was extracted by solid phase protein extraction. Gather the bay.
<실시예 4> 단백질 동정(Protein identification)Example 4 Protein Identification
나노급 액상 크로마토그래피 분석에는 nanoAcquity system(Waters Corporation, Milford, MA)을 사용하였고 사용한 컬럼은 Symmetry C18 5 μm, 2 mm × 180 μm 트랩컬럼과 BEH C18 1.7 μm, 25cm×75μm 분석용 역상 컬럼(Waters Corporation)이다. 앞에서 얻은 펩타이드는 0.1 % formic acid에 녹여서 분석한다. 이동상은 이동상 A 0.1 % formic/water, 이동상 B 0.1 % formic acid/ACN이 사용되었다. 10 μL/min의 유속으로 3분간 트랩컬럼으로 흘려준다. 이동상 B 3~40%농도로 300 nl/min 유속으로 360 분을 흘려서 펩타이드를 분리하고 이동상 B 90 %까지 15 분간 흘려 컬럼을 씻어 낸 후 컬럼 재 안정화를 위해 20 분간 이동상 B 3 %로 흘려준다. 컬럼 온도는 35 ℃를 유지하고, 보조 펌프를 이용하여 300 nL/min의 유속으로 100 fmol [Glu1]-Fibrinopeptide B/μL를 질량분석기의 나노 락 레퍼런스 스프레이로 이동시킨다. 모든 시료는 4반복 실험을 실시했다. 트립틱 펩타이드의 분석은 Q-Tof Premier mass spectrometer(Waters Corporation, Manchester, UK)를 이용하여 실시되었다. 질량분석기는 v-mode로 10,000 FWHM의 레졸루션을 가진다. 모든 분석은 양 이온 모드로 진행되었고 TOF 분석은 m/z 50~1990범위에서 분석되었다. 30 초에 한번씩 2가 전하의 [Glu1]-Fibrinopeptide B를 이용하여 실시간 질량 값을 보정하게 된다. 저 충돌 에너지 질량분석은 4 eV로 1 초간 데이터를 모으고 고 충돌 에너지 분석은 15 eV에서 40 eV로 에너지를 증가시키면서 1 초간 데이터를 모은다. 저 충돌에너지 분석과 고 충돌 에너지 분석간에는 0.1 초의 시간차를 두고 데이터를 모으게 된다. A nanoAcquity system (Waters Corporation, Milford, Mass.) Was used for nanoscale liquid chromatography analysis.The columns used were Symmetry C18 5 μm, 2 mm × 180 μm trap columns and BEH C18 1.7 μm, 25 cm × 75 μm analytical reversed phase columns (Waters). Corporation). The peptide obtained above is analyzed by dissolving in 0.1% formic acid. Mobile phase A 0.1% formic / water and mobile phase B 0.1% formic acid / ACN were used. Flow into the trap column for 3 minutes at a flow rate of 10 μL / min. Peptides were separated by a flow of 360 minutes at a flow rate of 300 nl / min at a concentration of 3-40% of mobile phase B, washed for 15 minutes to 90% of mobile phase B, and then flowed into mobile phase B 3% for 20 minutes to re-stabilize the column. The column temperature is maintained at 35 ° C., and 100 fmol [Glu 1] -Fibrinopeptide B / μL is transferred to the nanolock reference spray of the mass spectrometer at a flow rate of 300 nL / min using an auxiliary pump. All samples were subjected to four replicate experiments. Analysis of tryptic peptides was performed using a Q-Tof Premier mass spectrometer (Waters Corporation, Manchester, UK). The mass spectrometer has a resolution of 10,000 FWHM in v-mode. All analyzes were carried out in the positive ion mode and TOF analysis was performed in the range of m / z 50 ~ 1990. Once every 30 seconds, [Glu1] -Fibrinopeptide B of bivalent charge is used to correct the real-time mass values. Low collision energy mass spectrometry gathers data for 1 second at 4 eV and high impact energy analysis gathers data for 1 second while increasing energy from 15 eV to 40 eV. Data is collected with a time difference of 0.1 seconds between low collision energy analysis and high collision energy analysis.
<실시예 5> 상대적 정량분석(Relative quantificatuon)Example 5 Relative quantificatuon
액상 크로마토그래피 질량분석기로 얻어진 데이터는 ProteinLynx GlobalServer v2.3 (Waters Corporation)소프트웨어를 이용하여 분석하였다. 클러스터링과 노말라이즈 된 데이터로부터 ProteinLynx GlobalServer v2.3의 알고리즘을 이용하여 단백질을 동정한 후 동정된 결과의 정확한 질량값과 액상 크로마토그래피의 지연시간을 이용하여 클러스터링을 실시하였다 . 동정된 트립틱 펩타이드의 피크 강도(intensity)를 이용하여 각 단백질의 양을 계산하게 되고 이를 이용하여 각 군별로 단백질의 발현을 확인한다.Data obtained by liquid chromatography mass spectrometry was analyzed using ProteinLynx GlobalServer v2.3 (Waters Corporation) software. Proteins were identified from the clustered and normalized data using the ProteinLynx GlobalServer v2.3 algorithm and clustered using the exact mass values of the identified results and the liquid phase delay time. The amount of each protein is calculated using the peak intensities of the identified tryptic peptides, and the expression of the proteins is confirmed for each group using the peak intensities.
<실시예 6> 바이오마커 확인(Biomarker validation)Example 6 Biomarker validation
12명의 정상인과 5명의 IgA 신증 환자와 7명의 TGBM 환자의 뇨에서 엑소좀을 분리하여 anti-Aminopeptidase N, anti-Vasorin precursor, anti-Ceruloplasmin, anti-Alpha-1-antitrypsin, 항체를 이용하여 웨스턴 블랏을 실시하여 각 군에서 위 단백질의 발현 정도를 확인 하였다. 뇨 엑소좀에서 질환을 진단할 수 있는 바이오마커 발굴의 전체 실험과정은 도 2에 나타내었다. Exosomes were isolated from the urine of 12 normal, 5 IgA nephropathy, and 7 TGBM patients, and Western blots using anti-Aminopeptidase N, anti-Vasorin precursor, anti-Ceruloplasmin, anti-Alpha-1-antitrypsin, and antibodies. The degree of expression of gastric protein in each group was confirmed by performing the test. The entire experimental process of discovering biomarkers capable of diagnosing diseases in urine exosomes is shown in FIG. 2.
본 발명은 상기와 같은 실험을 수행한 결과, 단백질 분석은 LC-MS/MS를 이용하여 정상인 그룹(“Normal 그룹”)과 질병의 두 그룹(“IgAN 그룹”, “TGBM 그룹”)의 3회 반복 실험에서 총 2059개의 단백질이 동정되었으며 그 중의 일부 단백질을 표2 에 나타내었다.According to the present invention, as a result of the above experiment, protein analysis was performed three times in the normal group ("Normal group") and two groups of disease ("IgAN group" and "TGBM group") using LC-MS / MS. A total of 2059 proteins were identified in the repeated experiments, some of which are shown in Table 2.
표2. 실험에서 동정된 증감을 보이는 단백질Table 2. Proteins that Show Identified Changes in Experiments
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00000073.1IPI: IPI00000073.1
EGF Pro-epidermal growth factor precursorEGF Pro-epidermal growth factor precursor
1198.611198.61
1.00 1.00
--
1.19 1.19
IPI:IPI00247063.3IPI: IPI00247063.3
MME NeprilysinMME Neprilysin
1112.421112.42
1.00 1.00
--
0.75 0.75
IPI:IPI00788190.1IPI: IPI00788190.1
EPS8L2 similar to epidermal growth factor receptor pathway substrate 8-like protein 2EPS8L2 similar to epidermal growth factor receptor pathway substrate 8-like protein 2
851.67851.67
1.00 1.00
--
1.31 1.31
IPI:IPI00017601.1IPI: IPI00017601.1
CP Ceruloplasmin precursorCP Ceruloplasmin precursor
742.16742.16
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00017367.6IPI: IPI00017367.6
RDX Radixin isoform bRDX Radixin isoform b
728.13728.13
1.00 1.00
--
0.89 0.89
IPI:IPI00022463.1IPI: IPI00022463.1
TF Serotransferrin precursorTF Serotransferrin precursor
711.44711.44
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00798430.1IPI: IPI00798430.1
TF Transferrin variant (Fragment)TF Transferrin variant (Fragment)
711.44711.44
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00290337.7IPI: IPI00290337.7
EPS8 Uncharacterized protein EPS8EPS8 Uncharacterized protein EPS8
707.62707.62
1.00 1.00
--
0.84 0.84
IPI:IPI00008787.3IPI: IPI00008787.3
NAGLU Alpha-N-acetylglucosaminidase precursorNAGLU Alpha-N-acetylglucosaminidase precursor
691.64691.64
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00339269.1IPI: IPI00339269.1
HSPA6 Heat shock 70 kDa protein 6HSPA6 Heat shock 70 kDa protein 6
690.55690.55
1.00 1.00
--
1.30 1.30
IPI:IPI00220327.3IPI: IPI00220327.3
KRT1 Keratin, type II cytoskeletal 1KRT1 Keratin, type II cytoskeletal 1
679.34679.34
1.00 1.00
0.25 0.25
0.61 0.61
IPI:IPI00295988.4IPI: IPI00295988.4
PROM2 Prominin-2PROM2 Prominin-2
669.97669.97
1.00 1.00
--
1.27 1.27
IPI:IPI00246058.5IPI: IPI00246058.5
PDCD6IP PDCD6IP proteinPDCD6IP PDCD6IP protein
669.19669.19
1.00 1.00
0.11 0.11
1.06 1.06
IPI:IPI00871576.1IPI: IPI00871576.1
PDCD6IP Dopamine receptor interacting protein 4PDCD6IP Dopamine receptor interacting protein 4
663.3663.3
1.00 1.00
0.11 0.11
1.07 1.07
IPI:IPI00062047.5IPI: IPI00062047.5
KIF12 Kinesin-like protein KIF12KIF12 Kinesin-like protein KIF12
657.26657.26
1.00 1.00
--
0.92 0.92
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00004573.2IPI: IPI00004573.2
PIGR Polymeric immunoglobulin receptor precursorPIGR Polymeric immunoglobulin receptor precursor
631.19631.19
1.00 1.00
--
0.93 0.93
IPI:IPI00555656.1IPI: IPI00555656.1
Huntingtin interacting protein-1-related (Fragment)Huntingtin interacting protein-1-related (Fragment)
621.62621.62
1.00 1.00
--
2.16 2.16
IPI:IPI00746033.1IPI: IPI00746033.1
NAGLU 47 kDa proteinNAGLU 47 kDa protein
618.94618.94
1.00 1.00
--
1.55 1.55
IPI:IPI00018953.1IPI: IPI00018953.1
DPP4 Dipeptidyl peptidase 4DPP4 Dipeptidyl peptidase 4
589.84589.84
1.00 1.00
--
0.79 0.79
IPI:IPI00792032.1IPI: IPI00792032.1
ABCB1 P-glycoprotein 1ABCB1 P-glycoprotein 1
581.18581.18
1.00 1.00
--
1.02 1.02
IPI:IPI00027481.1IPI: IPI00027481.1
ABCB1 Multidrug resistance protein 1ABCB1 Multidrug resistance protein 1
578.8578.8
1.00 1.00
--
0.74 0.74
IPI:IPI00003765.1IPI: IPI00003765.1
CAPN7 Calpain-7CAPN7 Calpain-7
547.66547.66
1.00 1.00
--
1.05 1.05
IPI:IPI00177880.3IPI: IPI00177880.3
PROM2 CDNA FLJ34089 fis, clone FCBBF3006249, moderately similar to Mus musculus prominin-like protein mRNAPROM2 CDNA FLJ34089 fis, clone FCBBF3006249, moderately similar to Mus musculus prominin-like protein mRNA
542.58542.58
1.00 1.00
--
1.17 1.17
IPI:IPI00478530.1IPI: IPI00478530.1
KIF12 Kinesin family member 12KIF12 Kinesin family member 12
539.36539.36
1.00 1.00
--
1.06 1.06
IPI:IPI00794184.1IPI: IPI00794184.1
CP 97 kDa proteinCP 97 kDa protein
535.08535.08
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00304925.5IPI: IPI00304925.5
HSPA1A;HSPA1B Heat shock 70 kDa protein 1HSPA1A; HSPA1B Heat shock 70 kDa protein 1
519.91519.91
1.00 1.00
--
1.21 1.21
IPI:IPI00847536.1IPI: IPI00847536.1
HSPA1A;HSPA1B heat shock 70kDa protein 1BHSPA1A; HSPA1B heat shock 70kDa protein 1B
519.91519.91
1.00 1.00
--
1.19 1.19
IPI:IPI00845339.1IPI: IPI00845339.1
HSPA1A;HSPA1B heat shock 70kDa protein 1AHSPA1A; HSPA1B heat shock 70kDa protein 1A
519.91519.91
1.00 1.00
--
1.15 1.15
IPI:IPI00746183.2IPI: IPI00746183.2
UEVLD Isoform 1 of Ubiquitin-conjugating enzyme E2 variant 3UEVLD Isoform 1 of Ubiquitin-conjugating enzyme E2 variant 3
515.73515.73
1.00 1.00
--
0.92 0.92
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00005578.1IPI: IPI00005578.1
EHD4 EH domain-containing protein 4EHD4 EH domain-containing protein 4
463.22463.22
1.00 1.00
--
1.09 1.09
IPI:IPI00465028.7IPI: IPI00465028.7
TPI1 Isoform 1 of Triosephosphate isomeraseTPI1 Isoform 1 of Triosephosphate isomerase
459.21459.21
1.00 1.00
--
0.99 0.99
IPI:IPI00785050.1IPI: IPI00785050.1
Putative uncharacterized proteinPutative uncharacterized protein
458.65458.65
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00010418.4IPI: IPI00010418.4
MYO1C Myosin-IcMYO1C Myosin-Ic
455.53455.53
1.00 1.00
--
0.48 0.48
IPI:IPI00216171.3IPI: IPI00216171.3
ENO2 Gamma-enolaseENO2 Gamma-enolase
455455
1.00 1.00
--
0.71 0.71
IPI:IPI00384697.2IPI: IPI00384697.2
ALB Isoform 2 of Serum albumin precursorALB Isoform 2 of Serum albumin precursor
451.54451.54
1.00 1.00
4.00 4.00
1.86 1.86
IPI:IPI00414315.8IPI: IPI00414315.8
EPS8L2 Isoform 1 of Epidermal growth factor receptor kinase substrate 8-like protein 2EPS8L2 Isoform 1 of Epidermal growth factor receptor kinase substrate 8-like protein 2
449.28449.28
1.00 1.00
--
1.51 1.51
IPI:IPI00299088.3IPI: IPI00299088.3
BAIAP2 Isoform 1 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 1 of Brain-specific angiogenesis inhibitor 1-associated protein 2
449.07449.07
1.00 1.00
--
3.00 3.00
IPI:IPI00644120.1IPI: IPI00644120.1
BAIAP2 Isoform 2 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 2 of Brain-specific angiogenesis inhibitor 1-associated protein 2
449.07449.07
1.00 1.00
--
2.77 2.77
IPI:IPI00003269.1IPI: IPI00003269.1
DKFZp686D0972 hypothetical protein LOC345651DKFZp686D0972 hypothetical protein LOC345651
447.24447.24
1.00 1.00
--
1.09 1.09
IPI:IPI00553177.1IPI: IPI00553177.1
SERPINA1 Isoform 1 of Alpha-1-antitrypsin precursorSERPINA1 Isoform 1 of Alpha-1-antitrypsin precursor
446.13446.13
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00873444.1IPI: IPI00873444.1
RPS27A;UBC;UBB 79 kDa proteinRPS27A; UBC; UBB 79 kDa protein
443.55443.55
1.00 1.00
0.20 0.20
0.83 0.83
IPI:IPI00854806.1IPI: IPI00854806.1
IGKV1-5 IGKV1-5 proteinIGKV1-5 IGKV1-5 protein
443.17443.17
--
1.00 1.00
0.64 0.64
IPI:IPI00218407.6IPI: IPI00218407.6
ALDOB Fructose-bisphosphate aldolase BALDOB Fructose-bisphosphate aldolase B
442.98442.98
1.00 1.00
--
1.30 1.30
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00399007.5IPI: IPI00399007.5
IGHG2 Putative uncharacterized protein DKFZp686I04196 (Fragment)IGHG2 Putative uncharacterized protein DKFZp686I04196 (Fragment)
441.69441.69
--
1.00 1.00
0.43 0.43
IPI:IPI00644855.1IPI: IPI00644855.1
C1orf58 Chromosome 1 open reading frame 58C1orf58 Chromosome 1 open reading frame 58
437.68437.68
1.00 1.00
--
1.80 1.80
IPI:IPI00337415.9IPI: IPI00337415.9
GNAI1 Guanine nucleotide-binding protein G(i), alpha-1 subunitGNAI1 Guanine nucleotide-binding protein G (i), alpha-1 subunit
437.3437.3
1.00 1.00
--
1.07 1.07
IPI:IPI00798127.1IPI: IPI00798127.1
RPS27A;UBC;UBB ubiquitin CRPS27A; UBC; UBB ubiquitin C
436.16436.16
1.00 1.00
0.16 0.16
0.85 0.85
IPI:IPI00790784.2IPI: IPI00790784.2
SERPINA1 Isoform 2 of Alpha-1-antitrypsin precursorSERPINA1 Isoform 2 of Alpha-1-antitrypsin precursor
435.08435.08
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00165579.6IPI: IPI00165579.6
CNDP2 PP856CNDP2 PP856
433.91433.91
1.00 1.00
--
1.13 1.13
IPI:IPI00642333.1IPI: IPI00642333.1
BAIAP2 Isoform 6 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 6 of Brain-specific angiogenesis inhibitor 1-associated protein 2
433.61433.61
1.00 1.00
- -
2.69 2.69
IPI:IPI00185159.7IPI: IPI00185159.7
BAIAP2 Isoform 4 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 4 of Brain-specific angiogenesis inhibitor 1-associated protein 2
433.61433.61
1.00 1.00
--
1.46 1.46
IPI:IPI00441344.1IPI: IPI00441344.1
GLB1 Beta-galactosidase precursorGLB1 Beta-galactosidase precursor
433.01433.01
1.00 1.00
--
0.77 0.77
IPI:IPI00797646.3IPI: IPI00797646.3
GLB1 Beta-galactosidase (Fragment)GLB1 Beta-galactosidase (Fragment)
433.01433.01
1.00 1.00
--
0.50 0.50
IPI:IPI00479743.3IPI: IPI00479743.3
POTE2 protein expressed in prostate, ovary, testis, and placenta 2POTE2 protein expressed in prostate, ovary, testis, and placenta 2
432.29432.29
1.00 1.00
--
1.21 1.21
IPI:IPI00180292.5IPI: IPI00180292.5
BAIAP2 Isoform 5 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 5 of Brain-specific angiogenesis inhibitor 1-associated protein 2
431.62431.62
1.00 1.00
--
1.15 1.15
IPI:IPI00785200.1IPI: IPI00785200.1
Putative uncharacterized proteinPutative uncharacterized protein
430.24430.24
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00739539.5IPI: IPI00739539.5
LOC728378 Chimeric POTE-actin proteinLOC728378 Chimeric POTE-actin protein
428.33428.33
1.00 1.00
--
1.28 1.28
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00004503.5IPI: IPI00004503.5
LAMP1 lysosomal-associated membrane protein 1LAMP1 lysosomal-associated membrane protein 1
426.54426.54
1.00 1.00
--
1.00 1.00
IPI:IPI00305457.5IPI: IPI00305457.5
SERPINA1 PRO2275SERPINA1 PRO2275
424.86424.86
1.00 1.00
3.23 3.23
--
IPI:IPI00647603.1IPI: IPI00647603.1
BAIAP2 Isoform 3 of Brain-specific angiogenesis inhibitor 1-associated protein 2BAIAP2 Isoform 3 of Brain-specific angiogenesis inhibitor 1-associated protein 2
424.12424.12
1.00 1.00
--
0.76 0.76
IPI:IPI00219365.3IPI: IPI00219365.3
MSN MoesinMSN Moesin
423.34423.34
1.00 1.00
--
0.92 0.92
IPI:IPI00872814.1IPI: IPI00872814.1
MSN 68 kDa proteinMSN 68 kDa protein
423.34423.34
1.00 1.00
--
0.88 0.88
IPI:IPI00217906.3IPI: IPI00217906.3
GNAI2 Isoform 2 of Guanine nucleotide-binding protein G(i), alpha-2 subunitGNAI2 Isoform 2 of Guanine nucleotide-binding protein G (i), alpha-2 subunit
421.08421.08
1.00 1.00
--
0.97 0.97
IPI:IPI00555945.1IPI: IPI00555945.1
IGL@ IGL@ proteinIGL @ IGL @ protein
420.93420.93
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00217342.8IPI: IPI00217342.8
UEVLD Isoform 2 of Ubiquitin-conjugating enzyme E2 variant 3UEVLD Isoform 2 of Ubiquitin-conjugating enzyme E2 variant 3
420.53420.53
1.00 1.00
--
0.95 0.95
IPI:IPI00027438.2IPI: IPI00027438.2
FLOT1 Flotillin-1FLOT1 Flotillin-1
419.67419.67
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00217717.2IPI: IPI00217717.2
GGT1 Isoform 2 of Gamma-glutamyltranspeptidase 1 precursorGGT1 Isoform 2 of Gamma-glutamyltranspeptidase 1 precursor
419.38419.38
1.00 1.00
--
0.69 0.69
IPI:IPI00646907.1IPI: IPI00646907.1
SLC12A3 solute carrier family 12 (sodium/chloride transporters), member 3SLC12A3 solute carrier family 12 (sodium / chloride transporters), member 3
418.98418.98
1.00 1.00
--
0.64 0.64
IPI:IPI00216438.3IPI: IPI00216438.3
SLC12A3 Solute carrier family 12 member 3SLC12A3 Solute carrier family 12 member 3
418.98418.98
1.00 1.00
--
0.24 0.24
IPI:IPI00816555.1IPI: IPI00816555.1
IGLV2-14 IGLV2-14 proteinIGLV2-14 IGLV2-14 protein
418.93418.93
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00719452.1IPI: IPI00719452.1
IGL@ IGL@ proteinIGL @ IGL @ protein
415.4415.4
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00007682.2IPI: IPI00007682.2
ATP6V1A Vacuolar ATP synthase catalytic subunit AATP6V1A Vacuolar ATP synthase catalytic subunit A
415.25415.25
NormalNormal
Normal_highNormal_high
--
--
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00420088.1IPI: IPI00420088.1
KIAA0174 Isoform 2 of Uncharacterized protein KIAA0174KIAA0174 Isoform 2 of Uncharacterized protein KIAA0174
415.06415.06
1.00 1.00
0.14 0.14
1.46 1.46
IPI:IPI00829626.1IPI: IPI00829626.1
IGL@ IGL@ proteinIGL @ IGL @ protein
414.97414.97
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00218343.4IPI: IPI00218343.4
TUBA1C Tubulin alpha-1C chainTUBA1C Tubulin alpha-1C chain
413.63413.63
1.00 1.00
--
0.66 0.66
IPI:IPI00873229.1IPI: IPI00873229.1
UEVLD 42 kDa proteinUEVLD 42 kDa protein
412.57412.57
1.00 1.00
--
1.02 1.02
IPI:IPI00382938.3IPI: IPI00382938.3
IGLV4-3 IGLV4-3 proteinIGLV4-3 IGLV4-3 protein
411.54411.54
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00003362.2IPI: IPI00003362.2
HSPA5 HSPA5 proteinHSPA5 HSPA5 protein
410.4410.4
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00024660.1IPI: IPI00024660.1
KIAA0174 Isoform 1 of Uncharacterized protein KIAA0174KIAA0174 Isoform 1 of Uncharacterized protein
409.31409.31
1.00 1.00
0.17 0.17
1.58 1.58
IPI:IPI00386516.3IPI: IPI00386516.3
KIAA0174 KIAA0174 proteinKIAA0174 KIAA0174 protein
409.31409.31
1.00 1.00
0.16 0.16
1.57 1.57
IPI:IPI00550162.2IPI: IPI00550162.2
IGLV3-25 IGLV3-25 proteinIGLV3-25 IGLV3-25 protein
408.52408.52
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00797510.1IPI: IPI00797510.1
GNAI2 35 kDa proteinGNAI2 35 kDa protein
408.48408.48
1.00 1.00
--
1.01 1.01
IPI:IPI00798391.1IPI: IPI00798391.1
GNAI2 39 kDa proteinGNAI2 39 kDa protein
408.48408.48
1.00 1.00
--
0.97 0.97
IPI:IPI00794986.1IPI: IPI00794986.1
GNAI2 WUGSC:H_LUCA15.1 proteinGNAI2 WUGSC: H_LUCA15.1 protein
408.48408.48
1.00 1.00
--
0.95 0.95
IPI:IPI00025476.1IPI: IPI00025476.1
AMY1A;AMY1C;AMY2A;AMY1B Pancreatic alpha-amylase precursorAMY1A; AMY1C; AMY2A; AMY1B Pancreatic alpha-amylase precursor
407.93407.93
1.00 1.00
--
4.06 4.06
IPI:IPI00867501.1IPI: IPI00867501.1
TUBA1B Similar to Tubulin alpha-ubiquitous chainTUBA1B Similar to Tubulin alpha-ubiquitous chain
407.67407.67
1.00 1.00
--
0.79 0.79
IPI:IPI00387144.4IPI: IPI00387144.4
TUBA1B Tubulin alpha-1B chainTUBA1B Tubulin alpha-1B chain
407.67407.67
1.00 1.00
--
0.77 0.77
IPI:IPI00792677.1IPI: IPI00792677.1
TUBA1B 46 kDa proteinTUBA1B 46 kDa protein
407.67407.67
1.00 1.00
--
0.56 0.56
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00658130.1IPI: IPI00658130.1
IGL@ IGL@ proteinIGL @ IGL @ protein
396.3396.3
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00555577.1IPI: IPI00555577.1
THY1 Thy-1 cell surface antigen variant (Fragment)THY1 Thy-1 cell surface antigen variant (Fragment)
395.31395.31
1.00 1.00
--
0.60 0.60
IPI:IPI00478908.3IPI: IPI00478908.3
TUBA1C 29 kDa proteinTUBA1C 29 kDa protein
394.78394.78
1.00 1.00
--
0.78 0.78
IPI:IPI00018434.1IPI: IPI00018434.1
TSG101 Isoform 1 of Tumor susceptibility gene 101 proteinTSG101 Isoform 1 of Tumor susceptibility gene 101 protein
394.12394.12
1.00 1.00
--
0.76 0.76
IPI:IPI00718819.2IPI: IPI00718819.2
IGLC3;IGLV2-14;IGLC2;IGLC1;IGL@ IGLV2-14 proteinIGLC3; IGLV2-14; IGLC2; IGLC1; IGL @ IGLV2-14 protein
394.07394.07
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00795257.1IPI: IPI00795257.1
GAPDH 32 kDa proteinGAPDH 32 kDa protein
393.43393.43
1.00 1.00
--
0.85 0.85
IPI:IPI00807428.1IPI: IPI00807428.1
Putative uncharacterized proteinPutative uncharacterized protein
393.17393.17
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00830047.1IPI: IPI00830047.1
Uncharacterized protein ENSP00000374858 (Fragment)Uncharacterized protein ENSP00000374858 (Fragment)
391.69391.69
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00827875.1IPI: IPI00827875.1
Lambda-chain precursorLambda-chain precursor
390.42390.42
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00744476.6IPI: IPI00744476.6
IGL@ IGL@ proteinIGL @ IGL @ protein
388.78388.78
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00784935.1IPI: IPI00784935.1
Putative uncharacterized proteinPutative uncharacterized protein
388.78388.78
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00789134.1IPI: IPI00789134.1
GAPDH Glyceraldehyde 3-phosphate dehydrogenaseGAPDH Glyceraldehyde 3-phosphate dehydrogenase
388.29388.29
1.00 1.00
--
0.96 0.96
IPI:IPI00218414.5IPI: IPI00218414.5
CA2 Carbonic anhydrase 2CA2 Carbonic anhydrase 2
387.66387.66
1.00 1.00
--
0.89 0.89
IPI:IPI00740545.1IPI: IPI00740545.1
LOC653269 similar to Prostate, ovary, testis expressed protein on chromosome 2 isoform 2LOC653269 similar to Prostate, ovary, testis expressed protein on chromosome 2 isoform 2
387.64387.64
1.00 1.00
--
0.79 0.79
IPI:IPI00218474.5IPI: IPI00218474.5
ENO3 Beta-enolaseENO3 Beta-enolase
386.68386.68
1.00 1.00
--
3.63 3.63
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00647012.1IPI: IPI00647012.1
HSPA1A;HSPA1B Heat shock 70kDa protein 1AHSPA1A; HSPA1B Heat shock 70kDa protein 1A
367.55367.55
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00473011.3IPI: IPI00473011.3
HBD;HBB Hemoglobin subunit deltaHBD; HBB Hemoglobin subunit delta
366.74366.74
--
1.00 1.00
4.85 4.85
IPI:IPI00478003.1IPI: IPI00478003.1
A2M Alpha-2-macroglobulin precursorA2M Alpha-2-macroglobulin precursor
364.04364.04
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00441550.1IPI: IPI00441550.1
GLB1 Beta-galactosidase-related protein precursorGLB1 Beta-galactosidase-related protein precursor
362.89362.89
1.00 1.00
--
0.57 0.57
IPI:IPI00022892.2IPI: IPI00022892.2
THY1 Thy-1 membrane glycoprotein precursorTHY1 Thy-1 membrane glycoprotein precursor
361.81361.81
1.00 1.00
--
0.59 0.59
IPI:IPI00797221.3IPI: IPI00797221.3
GAPDH Glyceraldehyde-3-phosphate dehydrogenaseGAPDH Glyceraldehyde-3-phosphate dehydrogenase
360.95360.95
1.00 1.00
--
0.84 0.84
IPI:IPI00182728.2IPI: IPI00182728.2
VPS4B Vacuolar protein sorting-associating protein 4BVPS4B Vacuolar protein sorting-associating protein 4B
360.67360.67
1.00 1.00
0.07 0.07
0.78 0.78
IPI:IPI00289983.3IPI: IPI00289983.3
ACPP ACPP proteinACPP ACPP protein
358.26358.26
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00384938.1IPI: IPI00384938.1
IGHG1 Putative uncharacterized protein DKFZp686N02209IGHG1 Putative uncharacterized protein DKFZp686N02209
358.13358.13
--
1.00 1.00
0.94 0.94
IPI:IPI00065500.3IPI: IPI00065500.3
C1orf58 BRO1 domain-containing protein BROXC1orf58 BRO1 domain-containing protein BROX
357.19357.19
1.00 1.00
--
1.08 1.08
IPI:IPI00789376.1IPI: IPI00789376.1
KNG1 KNG1 proteinKNG1 KNG1 protein
356.73356.73
1.00 1.00
--
0.83 0.83
IPI:IPI00383071.1IPI: IPI00383071.1
LOC729708 Triosephosphate isomerase (Fragment)LOC729708 Triosephosphate isomerase (Fragment)
355.76355.76
1.00 1.00
--
0.86 0.86
IPI:IPI00396434.1IPI: IPI00396434.1
ACPP Prostatic acid phosphatase precursorACPP Prostatic acid phosphatase precursor
354.4354.4
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00423463.1 IPI: IPI00423463.1
IGHG1 Putative uncharacterized protein DKFZp686O01196IGHG1 Putative uncharacterized protein DKFZp686O01196
354.2354.2
--
1.00 1.00
0.95 0.95
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00787680.1IPI: IPI00787680.1
similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 2similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 2
353.67353.67
1.00 1.00
--
0.97 0.97
IPI:IPI00790896.1IPI: IPI00790896.1
GNB2 26 kDa proteinGNB2 26 kDa protein
351.94351.94
1.00 1.00
--
1.16 1.16
IPI:IPI00829944.1IPI: IPI00829944.1
IGHG1 IGHG1 proteinIGHG1 IGHG1 protein
350.78350.78
--
1.00 1.00
0.90 0.90
IPI:IPI00872379.1IPI: IPI00872379.1
ANXA5 36 kDa proteinANXA5 36 kDa protein
350.57350.57
1.00 1.00
--
0.59 0.59
IPI:IPI00329801.12IPI: IPI00329801.12
ANXA5 Annexin A5ANXA5 Annexin A5
350.57350.57
1.00 1.00
--
0.58 0.58
IPI:IPI00829767.1IPI: IPI00829767.1
IGHG2 Uncharacterized protein IGHG2 (Fragment)IGHG2 Uncharacterized protein IGHG2 (Fragment)
350.51350.51
--
1.00 1.00
0.44 0.44
IPI:IPI00423466.1IPI: IPI00423466.1
IGHG1 Putative uncharacterized protein DKFZp686H20196IGHG1 Putative uncharacterized protein DKFZp686H20196
348.53348.53
1.00 1.00
4.76 4.76
4.31 4.31
IPI:IPI00790302.1IPI: IPI00790302.1
GNB2 28 kDa proteinGNB2 28 kDa protein
347.94347.94
1.00 1.00
--
1.19 1.19
IPI:IPI00011134.2IPI: IPI00011134.2
HSPA7 Putative heat shock 70 kDa protein 7HSPA7 Putative heat shock 70 kDa protein 7
347.71347.71
1.00 1.00
--
1.34 1.34
IPI:IPI00796250.1IPI: IPI00796250.1
GNAI2 27 kDa proteinGNAI2 27 kDa protein
346.68346.68
1.00 1.00
--
0.97 0.97
IPI:IPI00872328.1IPI: IPI00872328.1
HBB Beta-globin gene from a thalassemia patientHBB Beta-globin gene from a thalassemia patient
345.5345.5
--
1.00 1.00
4.85 4.85
IPI:IPI00784817.1IPI: IPI00784817.1
Anti-RhD monoclonal T125 gamma1 heavy chain precursorAnti-RhD monoclonal T125 gamma1 heavy chain precursor
344.56344.56
1.00 1.00
4.35 4.35
4.26 4.26
IPI:IPI00761159.1IPI: IPI00761159.1
IGHM IGHM proteinIGHM IGHM protein
343.37343.37
1.00 1.00
4.76 4.76
4.06 4.06
IPI:IPI00807531.2IPI: IPI00807531.2
IGHG1 IGHG1 proteinIGHG1 IGHG1 protein
342.47342.47
--
1.00 1.00
0.79 0.79
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00796636.8IPI: IPI00796636.8
HBB Hemoglobin (Fragment)HBB Hemoglobin (Fragment)
340.64340.64
--
1.00 1.00
4.81 4.81
IPI:IPI00293088.4IPI: IPI00293088.4
GAA Uncharacterized protein GAAGAA Uncharacterized protein GAA
340.59340.59
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00398700.3IPI: IPI00398700.3
GNAO1 Guanine nucleotide-binding protein G(o) subunit alpha 2GNAO1 Guanine nucleotide-binding protein G (o) subunit alpha 2
340.2340.2
1.00 1.00
--
1.05 1.05
IPI:IPI00784828.1IPI: IPI00784828.1
Putative Uncharacterized protein DKFZp686C11235Putative Uncharacterized protein DKFZp686C11235
339.74339.74
1.00 1.00
4.76 4.76
4.18 4.18
IPI:IPI00815926.1IPI: IPI00815926.1
IGHG1 IGHG1 proteinIGHG1 IGHG1 protein
339.65339.65
1.00 1.00
5.26 5.26
4.71 4.71
IPI:IPI00004101.4IPI: IPI00004101.4
BHMT Betaine--homocysteine S-methyltransferase 1BHMT Betaine--homocysteine S-methyltransferase 1
339.64339.64
1.00 1.00
--
0.93 0.93
IPI:IPI00513830.1IPI: IPI00513830.1
ALDOB Fructose-bisphosphate aldolaseALDOB Fructose-bisphosphate aldolase
339.19339.19
1.00 1.00
--
1.32 1.32
IPI:IPI00024403.1IPI: IPI00024403.1
CPNE3 Copine-3CPNE3 Copine-3
336.53336.53
1.00 1.00
--
0.82 0.82
IPI:IPI00448925.3IPI: IPI00448925.3
IGHG1 IGHG1 proteinIGHG1 IGHG1 protein
336.47336.47
--
1.00 1.00
0.90 0.90
IPI:IPI00743241.1IPI: IPI00743241.1
RPS27A;UBC;UBB 44 kDa proteinRPS27A; UBC; UBB 44 kDa protein
335.03335.03
1.00 1.00
0.19 0.19
0.84 0.84
IPI:IPI00743650.1IPI: IPI00743650.1
RPS27A;UBC;UBB 44 kDa proteinRPS27A; UBC; UBB 44 kDa protein
335.03335.03
1.00 1.00
0.18 0.18
0.84 0.84
IPI:IPI00784810.1IPI: IPI00784810.1
IGHV4-31 proteinIGHV4-31 protein
333.43333.43
1.00 1.00
4.17 4.17
3.74 3.74
IPI:IPI00465248.5IPI: IPI00465248.5
ENO1 Isoform alpha-enolase of Alpha-enolaseENO1 Isoform alpha-enolase of Alpha-enolase
333.4333.4
1.00 1.00
--
0.79 0.79
IPI:IPI00744274.1IPI: IPI00744274.1
RPS27A;UBC;UBB 44 kDa proteinRPS27A; UBC; UBB 44 kDa protein
332.81332.81
1.00 1.00
0.19 0.19
0.83 0.83
IPI:IPI00645363.2IPI: IPI00645363.2
IGHG1 Putative uncharacterized protein DKFZp686P15220IGHG1 Putative uncharacterized protein DKFZp686P15220
332.5332.5
--
1.00 1.00
0.93 0.93
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00423464.1IPI: IPI00423464.1
IGHG1 Putative uncharacterized protein DKFZp686K03196IGHG1 Putative uncharacterized protein DKFZp686K03196
332.27332.27
--
1.00 1.00
0.90 0.90
IPI:IPI00448938.1IPI: IPI00448938.1
IGHG1 IGHG1 proteinIGHG1 IGHG1 protein
332.07332.07
--
1.00 1.00
0.90 0.90
IPI:IPI00013769.1IPI: IPI00013769.1
Alpha-enolase, lung specificAlpha-enolase, lung specific
330.56330.56
1.00 1.00
--
1.36 1.36
IPI:IPI00784822.1IPI: IPI00784822.1
IGHV4-31 IGHV4-31 proteinIGHV4-31 IGHV4-31 protein
330.18330.18
--
1.00 1.00
0.90 0.90
IPI:IPI00335421.4IPI: IPI00335421.4
FIGNL1 Isoform 1 of Fidgetin-like protein 1FIGNL1 Isoform 1 of Fidgetin-like protein 1
330.14330.14
1.00 1.00
--
0.78 0.78
IPI:IPI00221224.6IPI: IPI00221224.6
ANPEP Aminopeptidase NANPEP Aminopeptidase N
328.19328.19
1.00 1.00
0.05 0.05
0.65 0.65
IPI:IPI00784842.1IPI: IPI00784842.1
IGHV4-31 Putative uncharacterized protein DKFZp686G11190IGHV4-31 Putative uncharacterized protein DKFZp686G11190
327.93327.93
--
1.00 1.00
0.91 0.91
IPI:IPI00792712.1IPI: IPI00792712.1
RPS27A;UBC;UBB 39 kDa proteinRPS27A; UBC; UBB 39 kDa protein
327.63327.63
1.00 1.00
0.19 0.19
0.84 0.84
IPI:IPI00009030.1IPI: IPI00009030.1
LAMP2 Isoform LAMP-2A of Lysosome-associated membrane glycoprotein 2 precursorLAMP2 Isoform LAMP-2A of Lysosome-associated membrane glycoprotein 2 precursor
327.49327.49
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00216172.2IPI: IPI00216172.2
LAMP2 Lysosomal-associated membrane protein 2CLAMP2 Lysosomal-associated membrane protein 2C
327.49327.49
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00739827.1IPI: IPI00739827.1
LAMP2 Isoform LAMP-2B of Lysosome-associated membrane glycoprotein 2 precursorLAMP2 Isoform LAMP-2B of Lysosome-associated membrane glycoprotein 2 precursor
327.49327.49
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00816681.1IPI: IPI00816681.1
IGHM Hepatitis B virus receptor binding protein (Fragment)IGHM Hepatitis B virus receptor binding protein (Fragment)
322.91322.91
1.00 1.00
5.00 5.00
4.44 4.44
IPI:IPI00220281.3IPI: IPI00220281.3
GNAO1 Guanine nucleotide-binding protein G(o) subunit alpha 1GNAO1 Guanine nucleotide-binding protein G (o) subunit alpha 1
322.25322.25
1.00 1.00
--
1.11 1.11
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00794035.1IPI: IPI00794035.1
GNAI2 20 kDa proteinGNAI2 20 kDa protein
311.97311.97
1.00 1.00
--
1.00 1.00
IPI:IPI00795746.1IPI: IPI00795746.1
GNAI2 19 kDa proteinGNAI2 19 kDa protein
311.97311.97
1.00 1.00
--
0.95 0.95
IPI:IPI00400826.1IPI: IPI00400826.1
CLU clusterin isoform 1CLU clusterin isoform 1
311.5311.5
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00418813.2IPI: IPI00418813.2
CDNA FLJ46113 fis, clone TESTI2036285, highly similar to Rattus norvegicus ubiquitin CCDNA FLJ46113 fis, clone TESTI2036285, highly similar to Rattus norvegicus ubiquitin C
311.25311.25
1.00 1.00
0.18 0.18
0.72 0.72
IPI:IPI00785079.1IPI: IPI00785079.1
Putative Uncharacterized proteinPutative Uncharacterized protein
310.01310.01
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00784589.1IPI: IPI00784589.1
Putative Uncharacterized protein DKFZp781M0386Putative Uncharacterized protein DKFZp781M0386
309.1309.1
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00829640.1IPI: IPI00829640.1
IGL@ IGL@ proteinIGL @ IGL @ protein
309.01309.01
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00855706.1IPI: IPI00855706.1
FIGNL1 Isoform 2 of Fidgetin-like protein 1FIGNL1 Isoform 2 of Fidgetin-like protein 1
307.57307.57
1.00 1.00
--
0.68 0.68
IPI:IPI00784711.1IPI: IPI00784711.1
Putative Uncharacterized proteinPutative Uncharacterized protein
307.48307.48
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00031821.1IPI: IPI00031821.1
ITM2B Integral membrane protein 2BITM2B Integral membrane protein 2B
306.68306.68
1.00 1.00
--
0.64 0.64
IPI:IPI00719373.1IPI: IPI00719373.1
IGL@ IGL@ proteinIGL @ IGL @ protein
306.35306.35
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00479186.5IPI: IPI00479186.5
PKM2 Isoform M2 of Pyruvate kinase isozymes M1/M2PKM2 Isoform M2 of Pyruvate kinase isozymes M1 / M2
303.28303.28
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00789902.1IPI: IPI00789902.1
GPRC5C G protein-coupled receptor family C, group 5, member C isoform bGPRC5C G protein-coupled receptor family C, group 5, member C isoform b
302.78302.78
1.00 1.00
0.12 0.12
1.36 1.36
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00719280.2IPI: IPI00719280.2
RPS27A;UBC;UBB ubiquitin B precursorRPS27A; UBC; UBB ubiquitin B precursor
290.53290.53
1.00 1.00
0.19 0.19
0.84 0.84
IPI:IPI00798155.3IPI: IPI00798155.3
RPS27A;UBC;UBB Ubiquitin C splice variantRPS27A; UBC; UBB Ubiquitin C splice variant
290.53290.53
1.00 1.00
0.18 0.18
0.82 0.82
IPI:IPI00423461.3IPI: IPI00423461.3
IGHA2 Putative uncharacterized protein DKFZp686C02220 (Fragment)IGHA2 Putative uncharacterized protein DKFZp686C02220 (Fragment)
290.26290.26
1.00 1.00
0.99 0.99
1.39 1.39
IPI:IPI00852577.1IPI: IPI00852577.1
IGLC1 proteinIGLC1 protein
289.84289.84
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00847989.1IPI: IPI00847989.1
PKM2 Pyruvate kinase (Fragment)PKM2 Pyruvate kinase (Fragment)
289.12289.12
1.00 1.00
--
1.43 1.43
IPI:IPI00791026.1IPI: IPI00791026.1
MYO1C 56 kDa proteinMYO1C 56 kDa protein
289.07289.07
1.00 1.00
--
0.81 0.81
IPI:IPI00180675.4IPI: IPI00180675.4
TUBA1A Tubulin alpha-1A chainTUBA1A Tubulin alpha-1A chain
285.54285.54
1.00 1.00
--
0.75 0.75
IPI:IPI00794663.1IPI: IPI00794663.1
TUBA4A 48 kDa proteinTUBA4A 48 kDa protein
284.61284.61
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00007750.1IPI: IPI00007750.1
TUBA4A Tubulin alpha-4A chainTUBA4A Tubulin alpha-4A chain
284.61284.61
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00790633.1IPI: IPI00790633.1
RPS27A;UBC;UBB 25 kDa proteinRPS27A; UBC; UBB 25 kDa protein
282.66282.66
1.00 1.00
0.19 0.19
0.83 0.83
IPI:IPI00646909.2IPI: IPI00646909.2
TUBA8 Tubulin alpha-8 chainTUBA8 Tubulin alpha-8 chain
282.44282.44
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00787871.1IPI: IPI00787871.1
LOC729708 similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 1LOC729708 similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 1
281.63281.63
1.00 1.00
--
0.84 0.84
IPI:IPI00007221.1IPI: IPI00007221.1
SERPINA5 Plasma serine protease inhibitor precursorSERPINA5 Plasma serine protease inhibitor precursor
280.84280.84
1.00 1.00
--
0.67 0.67
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00642117.4IPI: IPI00642117.4
GNB1 Beta-subunit signal transducing proteins GS/GIGNB1 Beta-subunit signal transducing proteins GS / GI
280.47280.47
1.00 1.00
--
1.13 1.13
IPI:IPI00793953.1IPI: IPI00793953.1
TUBA8 Putative uncharacterized protein DKFZp686L04275 (Fragment)TUBA8 Putative uncharacterized protein DKFZp686L04275 (Fragment)
280.05280.05
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00787104.1IPI: IPI00787104.1
similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 1similar to Triosephosphate isomerase (TIM) (Triose-phosphate isomerase) isoform 1
279.54279.54
1.00 1.00
--
0.84 0.84
IPI:IPI00827694.1IPI: IPI00827694.1
UEVLD Isoform 4 of Ubiquitin-conjugating enzyme E2 variant 3UEVLD Isoform 4 of Ubiquitin-conjugating enzyme E2 variant 3
279.48279.48
1.00 1.00
--
0.99 0.99
IPI:IPI00031065.1IPI: IPI00031065.1
DNASE1 Deoxyribonuclease-1 precursorDNASE1 Deoxyribonuclease-1 precursor
279.47279.47
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00794925.1IPI: IPI00794925.1
RPS27A;UBC;UBB 21 kDa proteinRPS27A; UBC; UBB 21 kDa protein
279.06279.06
1.00 1.00
0.19 0.19
0.82 0.82
IPI:IPI00295414.7IPI: IPI00295414.7
COL15A1 Collagen alpha-1(XV) chain precursorCOL15A1 Collagen alpha-1 (XV) chain precursor
278.78278.78
1.00 1.00
--
1.99 1.99
IPI:IPI00795527.1IPI: IPI00795527.1
RPS27A;UBC;UBB 21 kDa proteinRPS27A; UBC; UBB 21 kDa protein
278.08278.08
1.00 1.00
0.19 0.19
0.81 0.81
IPI:IPI00737216.1IPI: IPI00737216.1
LOC652899 similar to Salivary alpha-amylase precursorLOC652899 similar to Salivary alpha-amylase precursor
277.03277.03
1.00 1.00
--
3.67 3.67
IPI:IPI00797969.1IPI: IPI00797969.1
GNB2 17 kDa proteinGNB2 17 kDa protein
275.94275.94
1.00 1.00
--
1.11 1.11
IPI:IPI00291928.8IPI: IPI00291928.8
RAB14 Ras-related protein Rab-14RAB14 Ras-related protein Rab-14
275.03275.03
1.00 1.00
--
1.11 1.11
IPI:IPI00792478.1IPI: IPI00792478.1
TUBA8 43 kDa proteinTUBA8 43 kDa protein
274.85274.85
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00639998.1IPI: IPI00639998.1
GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1
274.52274.52
1.00 1.00
--
1.15 1.15
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00657660.1IPI: IPI00657660.1
HBB Hemoglobin delta-beta fusion proteinHBB Hemoglobin delta-beta fusion protein
273.87273.87
--
1.00 1.00
4.62 4.62
IPI:IPI00829877.1IPI: IPI00829877.1
IGL@ IGL@ proteinIGL @ IGL @ protein
272.78272.78
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00095891.2IPI: IPI00095891.2
GNAS Isoform XLas-1 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLasGNAS Isoform XLas-1 of Guanine nucleotide-binding protein G (s) subunit alpha isoforms XLas
271.41271.41
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00556204.1IPI: IPI00556204.1
Eukaryotic translation elongation factor 1 alpha 2 variant (Fragment)Eukaryotic translation elongation factor 1 alpha 2 variant (Fragment)
270.05270.05
1.00 1.00
--
3.35 3.35
IPI:IPI00218164.1IPI: IPI00218164.1
MUC1 Isoform 3 of Mucin-1 precursorMUC1 Isoform 3 of Mucin-1 precursor
269.65269.65
--
1.00 1.00
8.85 8.85
IPI:IPI00788932.1IPI: IPI00788932.1
GNAL Guanine nucleotide binding proteinGNAL Guanine nucleotide binding protein
269.18269.18
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00796600.1IPI: IPI00796600.1
RPS27A;UBC;UBB 18 kDa proteinRPS27A; UBC; UBB 18 kDa protein
267.56267.56
1.00 1.00
0.19 0.19
0.83 0.83
IPI:IPI00867545.1IPI: IPI00867545.1
MUC1 isoform M6MUC1 isoform M6
267.43267.43
1.00 1.00
--
0.75 0.75
IPI:IPI00793810.1IPI: IPI00793810.1
RPS27A;UBC;UBB 19 kDa proteinRPS27A; UBC; UBB 19 kDa protein
266.84266.84
1.00 1.00
0.19 0.19
0.82 0.82
IPI:IPI00796007.1IPI: IPI00796007.1
RPS27A;UBC;UBB 17 kDa proteinRPS27A; UBC; UBB 17 kDa protein
264.75264.75
1.00 1.00
0.19 0.19
0.84 0.84
IPI:IPI00797400.1IPI: IPI00797400.1
RPS27A;UBC;UBB 19 kDa proteinRPS27A; UBC; UBB 19 kDa protein
264.75264.75
1.00 1.00
0.19 0.19
0.81 0.81
IPI:IPI00794211.1IPI: IPI00794211.1
RPS27A;UBC;UBB 18 kDa proteinRPS27A; UBC; UBB 18 kDa protein
264.75264.75
1.00 1.00
0.19 0.19
0.80 0.80
IPI:IPI00830113.2IPI: IPI00830113.2
HBB 19 kDa proteinHBB 19 kDa protein
264.14264.14
--
1.00 1.00
5.26 5.26
IPI:IPI00789823.1IPI: IPI00789823.1
RPS27A;UBC;UBB 16 kDa proteinRPS27A; UBC; UBB 16 kDa protein
263.36263.36
1.00 1.00
0.19 0.19
0.82 0.82
IPI:IPI00640949.1IPI: IPI00640949.1
GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1
263.01263.01
1.00 1.00
--
1.20 1.20
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00640462.1IPI: IPI00640462.1
GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1GNB1 Guanine nucleotide binding protein (G protein), beta polypeptide 1
261.63261.63
1.00 1.00
--
1.13 1.13
IPI:IPI00784990.2IPI: IPI00784990.2
RPS27A;UBC;UBB Ubiquitin C splice variantRPS27A; UBC; UBB Ubiquitin C splice variant
261.51261.51
1.00 1.00
0.19 0.19
0.84 0.84
IPI:IPI00012451.3IPI: IPI00012451.3
GNB4 Guanine nucleotide-binding protein subunit beta-4GNB4 Guanine nucleotide-binding protein subunit beta-4
260.68260.68
1.00 1.00
--
1.13 1.13
IPI:IPI00645255.1IPI: IPI00645255.1
GDI2 GDP dissociation inhibitor 2GDI2 GDP dissociation inhibitor 2
260.03260.03
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00218163.1IPI: IPI00218163.1
MUC1 Isoform 2 of Mucin-1 precursorMUC1 Isoform 2 of Mucin-1 precursor
257.97257.97
--
1.00 1.00
8.58 8.58
IPI:IPI00218165.1IPI: IPI00218165.1
MUC1 Isoform 4 of Mucin-1 precursorMUC1 Isoform 4 of Mucin-1 precursor
257.97257.97
--
1.00 1.00
8.33 8.33
IPI:IPI00013955.1IPI: IPI00013955.1
MUC1 Isoform 1 of Mucin-1 precursorMUC1 Isoform 1 of Mucin-1 precursor
257.97257.97
--
1.00 1.00
8.33 8.33
IPI:IPI00607844.1IPI: IPI00607844.1
MUC1 Mucin 1, cell surface associatedMUC1 Mucin 1, cell surface associated
257.97257.97
1.00 1.00
0.10 0.10
0.92 0.92
IPI:IPI00829896.1IPI: IPI00829896.1
HBD Hemoglobin Lepore-Baltimore (Fragment)HBD Hemoglobin Lepore-Baltimore (Fragment)
255.62255.62
--
1.00 1.00
4.71 4.71
IPI:IPI00816229.1IPI: IPI00816229.1
ACTA2 ACTA2 protein (Fragment)ACTA2 ACTA2 protein (Fragment)
255.2255.2
1.00 1.00
0.15 0.15
1.26 1.26
IPI:IPI00291866.5IPI: IPI00291866.5
SERPING1 Plasma protease C1 inhibitor precursorSERPING1 Plasma protease C1 inhibitor precursor
252.16252.16
1.00 1.00
--
1.97 1.97
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00556287.1IPI: IPI00556287.1
Putative Uncharacterized proteinPutative Uncharacterized protein
250.96250.96
--
1.00 1.00
0.79 0.79
IPI:IPI00793848.1IPI: IPI00793848.1
CLU 54 kDa proteinCLU 54 kDa protein
249.02249.02
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00794488.1IPI: IPI00794488.1
GNAI2 12 kDa proteinGNAI2 12 kDa protein
248.73248.73
1.00 1.00
--
0.80 0.80
IPI:IPI00013945.1IPI: IPI00013945.1
UMOD Isoform 1 of Uromodulin precursorUMOD Isoform 1 of Uromodulin precursor
248.09248.09
1.00 1.00
0.76 0.76
1.34 1.34
IPI:IPI00640271.1IPI: IPI00640271.1
UMOD 74 kDa proteinUMOD 74 kDa protein
248.09248.09
1.00 1.00
0.74 0.74
1.34 1.34
IPI:IPI00032561.1IPI: IPI00032561.1
CAB39 Calcium-binding protein 39CAB39 Calcium-binding protein 39
247.93247.93
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00793330.1IPI: IPI00793330.1
RPS27A;UBC;UBB 12 kDa proteinRPS27A; UBC; UBB 12 kDa protein
247.23247.23
1.00 1.00
0.17 0.17
0.82 0.82
IPI:IPI00456429.3IPI: IPI00456429.3
UBA52 ubiquitin and ribosomal protein L40 precursorUBA52 ubiquitin and ribosomal protein L40 precursor
246.39246.39
1.00 1.00
0.19 0.19
0.81 0.81
IPI:IPI00759806.1IPI: IPI00759806.1
ENO1 Isoform MBP-1 of Alpha-enolaseENO1 Isoform MBP-1 of Alpha-enolase
246.28246.28
1.00 1.00
--
1.46 1.46
IPI:IPI00008964.3IPI: IPI00008964.3
RAB1B Ras-related protein Rab-1BRAB1B Ras-related protein Rab-1B
245.38245.38
1.00 1.00
--
0.71 0.71
IPI:IPI00300562.2IPI: IPI00300562.2
RAB3B Ras-related protein Rab-3BRAB3B Ras-related protein Rab-3B
245.02245.02
1.00 1.00
--
0.99 0.99
IPI:IPI00737338.1IPI: IPI00737338.1
UMOD Isoform 2 of Uromodulin precursorUMOD Isoform 2 of Uromodulin precursor
244.98244.98
1.00 1.00
0.88 0.88
1.21 1.21
IPI:IPI00744076.1IPI: IPI00744076.1
UMOD Isoform 3 of Uromodulin precursorUMOD Isoform 3 of Uromodulin precursor
244.45244.45
1.00 1.00
0.82 0.82
1.35 1.35
IPI:IPI00179330.6IPI: IPI00179330.6
RPS27A;UBC;UBB ubiquitin and ribosomal protein S27a precursorRPS27A; UBC; UBB ubiquitin and ribosomal protein S27a precursor
244.41244.41
1.00 1.00
0.19 0.19
0.82 0.82
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00795461.1IPI: IPI00795461.1
CAPN7 18 kDa proteinCAPN7 18 kDa protein
243.8243.8
1.00 1.00
--
0.73 0.73
IPI:IPI00646415.1IPI: IPI00646415.1
RAB14 20 kDa proteinRAB14 20 kDa protein
243.75243.75
1.00 1.00
--
0.98 0.98
IPI:IPI00654754.1IPI: IPI00654754.1
RPS27A;UBC;UBB RPS27A proteinRPS27A; UBC; UBB RPS27A protein
243.53243.53
1.00 1.00
0.20 0.20
0.93 0.93
IPI:IPI00556459.1IPI: IPI00556459.1
SERPING1 Serine/cysteine proteinase inhibitor clade G member 1 splice variant 2 (Fragment)SERPING1 Serine / cysteine proteinase inhibitor clade G member 1 splice variant 2 (Fragment)
243.4243.4
1.00 1.00
--
1.92 1.92
IPI:IPI00395488.2IPI: IPI00395488.2
VASN Vasorin precursorVASN Vasorin precursor
242.49242.49
1.00 1.00
--
3.49 3.49
IPI:IPI00008603.1IPI: IPI00008603.1
ACTA2 Actin, aortic smooth muscleACTA2 Actin, aortic smooth muscle
240.99240.99
1.00 1.00
0.08 0.08
1.26 1.26
IPI:IPI00792139.1IPI: IPI00792139.1
RPS27A;UBC;UBB 9 kDa proteinRPS27A; UBC; UBB 9 kDa protein
240.24240.24
1.00 1.00
0.18 0.18
0.93 0.93
IPI:IPI00646491.2IPI: IPI00646491.2
GNAS Isoform XLas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLasGNAS Isoform XLas-2 of Guanine nucleotide-binding protein G (s) subunit alpha isoforms XLas
239.84239.84
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00004416.1IPI: IPI00004416.1
CHMP2A Charged multivesicular body protein 2aCHMP2A Charged multivesicular body protein 2a
239.77239.77
1.00 1.00
--
0.95 0.95
IPI:IPI00478874.1IPI: IPI00478874.1
SDCBP syntenin isoform 2SDCBP syntenin isoform 2
239.64239.64
1.00 1.00
0.08 0.08
0.95 0.95
IPI:IPI00869217.1IPI: IPI00869217.1
LOC645367 Similar to Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2) (Gammaglutamyltransferase 1) (CD224 antigen) [Contains: Gammaglutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain]. Isoform 2LOC645367 Similar to Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2) (Gammaglutamyltransferase 1) (CD224 antigen) [Contains: Gammaglutamyltranspeptidase 1 heavy chain; Gamma-glutamyltranspeptidase 1 light chain]. Isoform 2
238.89238.89
1.00 1.00
--
0.64 0.64
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00607569.1IPI: IPI00607569.1
MUC1 mucin 1 isoform 2 precursorMUC1 mucin 1 isoform 2 precursor
238.62238.62
1.00 1.00
0.14 0.14
1.23 1.23
IPI:IPI00607673.2IPI: IPI00607673.2
MUC1 MUC1 isoform Z-LSPMUC1 MUC1 isoform Z-LSP
238.62238.62
1.00 1.00
0.13 0.13
1.19 1.19
IPI:IPI00385173.2IPI: IPI00385173.2
MUC1 mucin 1 isoform 3 precursorMUC1 mucin 1 isoform 3 precursor
238.62238.62
1.00 1.00
0.13 0.13
1.14 1.14
IPI:IPI00218170.1IPI: IPI00218170.1
MUC1 Isoform 8 of Mucin-1 precursorMUC1 Isoform 8 of Mucin-1 precursor
238.62238.62
1.00 1.00
0.11 0.11
0.96 0.96
IPI:IPI00218169.1IPI: IPI00218169.1
MUC1 Isoform 7 of Mucin-1 precursorMUC1 Isoform 7 of Mucin-1 precursor
238.62238.62
1.00 1.00
0.11 0.11
0.95 0.95
IPI:IPI00873004.1IPI: IPI00873004.1
UBA52 11 kDa proteinUBA52 11 kDa protein
237.63237.63
1.00 1.00
0.20 0.20
0.93 0.93
IPI:IPI00382606.1IPI: IPI00382606.1
F7 Factor VII active site mutant immunoconjugateF7 Factor VII active site mutant immunoconjugate
236.89236.89
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00657915.1IPI: IPI00657915.1
EHD1 41 kDa proteinEHD1 41 kDa protein
236.83236.83
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00216791.2IPI: IPI00216791.2
SLC12A1 SLC12A1 proteinSLC12A1 SLC12A1 protein
236.5236.5
1.00 1.00
--
1.12 1.12
IPI:IPI00745461.1IPI: IPI00745461.1
VASN Uncharacterized protein VASNVASN Uncharacterized protein VASN
235.47235.47
1.00 1.00
--
3.29 3.29
IPI:IPI00419932.4IPI: IPI00419932.4
RAB12 Putative Ras-related protein Rab-12RAB12 Putative Ras-related protein Rab-12
235.04235.04
1.00 1.00
--
1.55 1.55
IPI:IPI00025416.3IPI: IPI00025416.3
ACTG2 Actin, gamma-enteric smooth muscleACTG2 Actin, gamma-enteric smooth muscle
234.71234.71
1.00 1.00
0.08 0.08
1.26 1.26
IPI:IPI00218168.1IPI: IPI00218168.1
MUC1 Isoform 6 of Mucin-1 precursorMUC1 Isoform 6 of Mucin-1 precursor
232.19232.19
1.00 1.00
0.10 0.10
0.90 0.90
IPI:IPI00299086.3IPI: IPI00299086.3
SDCBP Syntenin-1SDCBP Syntenin-1
231.69231.69
1.00 1.00
0.12 0.12
0.60 0.60
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00640867.1IPI: IPI00640867.1
GNAS GNAS complex locus isoform fGNAS GNAS complex locus isoform f
231.25231.25
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00514055.1IPI: IPI00514055.1
GNAS Isoform Gnas-1 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortGNAS Isoform Gnas-1 of Guanine nucleotide-binding protein G (s) subunit alpha isoforms short
231.25231.25
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00738905.1IPI: IPI00738905.1
LOC648749 similar to Pancreatic alpha-amylase precursorLOC648749 similar to Pancreatic alpha-amylase precursor
231.03231.03
1.00 1.00
--
2.53 2.53
IPI:IPI00006662.1IPI: IPI00006662.1
APOD Apolipoprotein D precursorAPOD Apolipoprotein D precursor
230.36230.36
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00479018.2IPI: IPI00479018.2
SDCBP Syntenin isoform 3SDCBP Syntenin isoform 3
230.17230.17
1.00 1.00
0.11 0.11
0.59 0.59
IPI:IPI00021263.3IPI: IPI00021263.3
YWHAZ 14-3-3 protein zeta/deltaYWHAZ 14-3-3 protein zeta / delta
229.9229.9
1.00 1.00
--
0.81 0.81
IPI:IPI00789337.1IPI: IPI00789337.1
YWHAZ Tyrosine 3-monooxygenasea/tryptophan 5-monooxygenase activation protein zetaYWHAZ Tyrosine 3-monooxygenasea / tryptophan 5-monooxygenase activation protein zeta
229.85229.85
1.00 1.00
--
1.07 1.07
IPI:IPI00852677.1IPI: IPI00852677.1
MUC1 isoform T11MUC1 isoform T11
229.03229.03
1.00 1.00
0.11 0.11
0.99 0.99
IPI:IPI00855927.1IPI: IPI00855927.1
MUC1 MUC1 isoform M8MUC1 MUC1 isoform M8
229.03229.03
1.00 1.00
0.11 0.11
0.96 0.96
IPI:IPI00335314.3IPI: IPI00335314.3
TUBA4A Uncharacterized protein TUBA4ATUBA4A Uncharacterized protein TUBA4A
228.05228.05
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00025277.5IPI: IPI00025277.5
PDCD6 Programmed cell death protein 6PDCD6 Programmed cell death protein 6
227.45227.45
1.00 1.00
--
0.80 0.80
IPI:IPI00166768.3IPI: IPI00166768.3
TUBA1C TUBA1C proteinTUBA1C TUBA1C protein
226.01226.01
1.00 1.00
--
0.58 0.58
IPI:IPI00027223.2IPI: IPI00027223.2
IDH1 Isocitrate dehydrogenase [NADP] cytoplasmicIDH1 Isocitrate dehydrogenase [NADP] cytoplasmic
225.71225.71
1.00 1.00
--
1.09 1.09
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00215997.5IPI: IPI00215997.5
CD9 CD9 antigenCD9 CD9 antigen
224.74224.74
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00012113.1IPI: IPI00012113.1
UPK2 Uroplakin-2 precursorUPK2 Uroplakin-2 precursor
224.61224.61
1.00 1.00
--
1.17 1.17
IPI:IPI00027452.1IPI: IPI00027452.1
SLC2A5 Solute carrier family 2, facilitated glucose transporter member 5SLC2A5 Solute carrier family 2, facilitated glucose transporter member 5
224.45224.45
1.00 1.00
--
1.19 1.19
IPI:IPI00016513.5IPI: IPI00016513.5
RAB10 Ras-related protein Rab-10RAB10 Ras-related protein Rab-10
223.88223.88
1.00 1.00
--
0.70 0.70
IPI:IPI00305551.3IPI: IPI00305551.3
GNA11 Guanine nucleotide-binding protein subunit alpha-11GNA11 Guanine nucleotide-binding protein subunit alpha-11
223.85223.85
1.00 1.00
--
0.99 0.99
IPI:IPI00023006.1IPI: IPI00023006.1
ACTC1 Actin, alpha cardiac muscle 1ACTC1 Actin, alpha cardiac muscle 1
223.06223.06
1.00 1.00
0.08 0.08
1.26 1.26
IPI:IPI00414057.3IPI: IPI00414057.3
ACTA1 Actin alpha 1 skeletal muscle proteinACTA1 Actin alpha 1 skeletal muscle protein
222.53222.53
1.00 1.00
--
1.68 1.68
IPI:IPI00017454.3IPI: IPI00017454.3
TUBA4B Tubulin alpha-4 chainTUBA4B Tubulin alpha-4 chain
221.89221.89
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00219826.1IPI: IPI00219826.1
TSG101 Isoform 2 of Tumor susceptibility gene 101 proteinTSG101 Isoform 2 of Tumor susceptibility gene 101 protein
220.24220.24
1.00 1.00
--
0.89 0.89
IPI:IPI00555900.1IPI: IPI00555900.1
FKSG30 Kappa-actinFKSG30 Kappa-actin
219.71219.71
1.00 1.00
--
1.11 1.11
IPI:IPI00028481.1IPI: IPI00028481.1
RAB8A Ras-related protein Rab-8ARAB8A Ras-related protein Rab-8A
219.6219.6
1.00 1.00
--
0.67 0.67
IPI:IPI00000695.1IPI: IPI00000695.1
GNA14 Guanine nucleotide-binding protein alpha-14 subunitGNA14 Guanine nucleotide-binding protein alpha-14 subunit
218.41218.41
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00794699.1IPI: IPI00794699.1
SDCBP 27 kDa proteinSDCBP 27 kDa protein
218.29218.29
1.00 1.00
0.11 0.11
0.60 0.60
IPI:IPI00100796.4IPI: IPI00100796.4
CHMP5 Charged multivesicular body protein 5CHMP5 Charged multivesicular body protein 5
216.38216.38
1.00 1.00
--
2.32 2.32
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00869052.1IPI: IPI00869052.1
Similar to Elongation factor 1-alpha 1Similar to Elongation factor 1-alpha 1
216.09216.09
1.00 1.00
--
0.41 0.41
IPI:IPI00021428.1IPI: IPI00021428.1
ACTA1 Actin, alpha skeletal muscleACTA1 Actin, alpha skeletal muscle
214.37214.37
1.00 1.00
0.08 0.08
1.23 1.23
IPI:IPI00786945.1IPI: IPI00786945.1
LOC727848 similar to actin-like proteinLOC727848 similar to actin-like protein
214.32214.32
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00871597.1IPI: IPI00871597.1
MASP2 21 kDa proteinMASP2 21 kDa protein
209.96209.96
1.00 1.00
--
1.68 1.68
IPI:IPI00000816.1IPI: IPI00000816.1
YWHAE 14-3-3 protein epsilonYWHAE 14-3-3 protein epsilon
208.64208.64
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00646265.1IPI: IPI00646265.1
AMY1A;AMY1C;AMY2A;AMY1B Amylase, alpha 1BAMY1A; AMY1C; AMY2A; AMY1B Amylase, alpha 1B
208.1208.1
1.00 1.00
--
2.56 2.56
IPI:IPI00795937.1IPI: IPI00795937.1
CD9 15 kDa proteinCD9 15 kDa protein
207.74207.74
1.00 1.00
--
1.60 1.60
IPI:IPI00014363.1IPI: IPI00014363.1
BHMT2 Betaine--homocysteine S-methyltransferase 2BHMT2 Betaine--homocysteine S-methyltransferase 2
207.13207.13
1.00 1.00
--
1.46 1.46
IPI:IPI00306378.5IPI: IPI00306378.5
MASP2 Isoform 2 of Mannan-binding lectin serine protease 2 precursorMASP2 Isoform 2 of Mannan-binding lectin serine protease 2 precursor
207.1207.1
1.00 1.00
--
1.46 1.46
IPI:IPI00791558.1IPI: IPI00791558.1
HBB Delta-hemoglobinHBB Delta-hemoglobin
207.04207.04
--
1.00 1.00
4.53 4.53
IPI:IPI00790892.1IPI: IPI00790892.1
ENO2 6 kDa proteinENO2 6 kDa protein
206.95206.95
1.00 1.00
--
0.68 0.68
IPI:IPI00871366.1IPI: IPI00871366.1
RAB1B Small GTP-binding proteinRAB1B Small GTP-binding protein
206.62206.62
1.00 1.00
--
0.66 0.66
IPI:IPI00006395.1IPI: IPI00006395.1
GNAL Guanine nucleotide-binding protein G(olf) subunit alphaGNAL Guanine nucleotide-binding protein G (olf) subunit alpha
206.29206.29
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00032808.1IPI: IPI00032808.1
RAB3D Ras-related protein Rab-3DRAB3D Ras-related protein Rab-3D
206.09206.09
1.00 1.00
--
0.98 0.98
IPI:IPI00073180.8IPI: IPI00073180.8
RAB37 Ras-related protein Rab-37RAB37 Ras-related protein Rab-37
204.67204.67
TGBMTGBM
--
--
TGBM_highTGBM_high
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00025447.8IPI: IPI00025447.8
EEF1A1 Elongation factor 1-alphaEEF1A1 Elongation factor 1-alpha
198.97198.97
1.00 1.00
--
2.53 2.53
IPI:IPI00472724.1IPI: IPI00472724.1
Elongation factor 1-alphaElongation factor 1-alpha
197.03197.03
1.00 1.00
--
4.62 4.62
IPI:IPI00185072.10IPI: IPI00185072.10
UEVLD Isoform 5 of Ubiquitin-conjugating enzyme E2 variant 3UEVLD Isoform 5 of Ubiquitin-conjugating enzyme E2 variant 3
195.5195.5
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00791608.1IPI: IPI00791608.1
HSPA8 20 kDa proteinHSPA8 20 kDa protein
195.26195.26
1.00 1.00
--
0.57 0.57
IPI:IPI00748022.1IPI: IPI00748022.1
P704P similar to actin-like proteinP704P similar to actin-like protein
195.04195.04
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00869004.1IPI: IPI00869004.1
SERPINA1 Isoform 3 of Alpha-1-antitrypsin precursorSERPINA1 Isoform 3 of Alpha-1-antitrypsin precursor
194.98194.98
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00793344.1IPI: IPI00793344.1
YWHAE 22 kDa proteinYWHAE 22 kDa protein
194.66194.66
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00382804.1IPI: IPI00382804.1
EEF1A1 EEF1A protein (Fragment)EEF1A1 EEF1A protein (Fragment)
194.36194.36
1.00 1.00
--
3.60 3.60
IPI:IPI00793922.1IPI: IPI00793922.1
GAPDH 9 kDa proteinGAPDH 9 kDa protein
192.47192.47
1.00 1.00
--
0.69 0.69
IPI:IPI00410714.5IPI: IPI00410714.5
HBA2;HBA1 Hemoglobin subunit alphaHBA2; HBA1 Hemoglobin subunit alpha
192.32192.32
--
1.00 1.00
6.62 6.62
IPI:IPI00853068.1IPI: IPI00853068.1
HBA2;HBA1 Alpha 2 globin variant (Fragment)HBA2; HBA1 Alpha 2 globin variant (Fragment)
192.32192.32
--
1.00 1.00
6.75 6.75
IPI:IPI00798392.1IPI: IPI00798392.1
RAB37 26 kDa proteinRAB37 26 kDa protein
190.35190.35
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00396485.3IPI: IPI00396485.3
EEF1A1 Elongation factor 1-alpha 1EEF1A1 Elongation factor 1-alpha 1
190.04190.04
1.00 1.00
--
1.23 1.23
IPI:IPI00016373.3IPI: IPI00016373.3
RAB13 Ras-related protein Rab-13RAB13 Ras-related protein Rab-13
189.36189.36
1.00 1.00
--
0.73 0.73
IPI:IPI00794523.2IPI: IPI00794523.2
ACTG1;PSPHL ACTG1 proteinACTG1; PSPHL ACTG1 protein
188.69188.69
1.00 1.00
0.12 0.12
1.20 1.20
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00646632.3IPI: IPI00646632.3
CNDP2 28 kDa proteinCNDP2 28 kDa protein
187.84187.84
1.00 1.00
--
1.00 1.00
IPI:IPI00022134.1IPI: IPI00022134.1
RAB1B Putative small GTP-binding proteinRAB1B Putative small GTP-binding protein
187.19187.19
1.00 1.00
--
0.72 0.72
IPI:IPI00791421.1IPI: IPI00791421.1
RAB37 21 kDa proteinRAB37 21 kDa protein
186.39186.39
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00552303.3IPI: IPI00552303.3
ANXA11 Annexin A11ANXA11 Annexin A11
185.44185.44
1.00 1.00
--
3.53 3.53
IPI:IPI00795002.1IPI: IPI00795002.1
TUBA1C 19 kDa proteinTUBA1C 19 kDa protein
185.04185.04
1.00 1.00
--
0.76 0.76
IPI:IPI00515047.2IPI: IPI00515047.2
ACTA1 Actin, alpha 1, skeletal muscleACTA1 Actin, alpha 1, skeletal muscle
184.34184.34
1.00 1.00
0.09 0.09
1.34 1.34
IPI:IPI00010896.3IPI: IPI00010896.3
CLIC1 Chloride intracellular channel protein 1CLIC1 Chloride intracellular channel protein 1
184.31184.31
1.00 1.00
--
0.84 0.84
IPI:IPI00023673.1IPI: IPI00023673.1
LGALS3BP Galectin-3-binding protein precursorLGALS3BP Galectin-3-binding protein precursor
183.02183.02
1.00 1.00
0.15 0.15
0.38 0.38
IPI:IPI00413344.3IPI: IPI00413344.3
CFL2 Cofilin-2CFL2 Cofilin-2
181.25181.25
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00790499.1IPI: IPI00790499.1
RAB37 21 kDa proteinRAB37 21 kDa protein
180.41180.41
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00419585.9IPI: IPI00419585.9
PPIA;PPIAL3;LOC654188 Peptidyl-prolyl cis-trans isomerase APPIA; PPIAL3; LOC654188 Peptidyl-prolyl cis-trans isomerase A
179.82179.82
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00785140.2IPI: IPI00785140.2
Uncharacterized protein ENSP00000330714Uncharacterized protein ENSP00000330714
179.04179.04
1.00 1.00
--
0.92 0.92
IPI:IPI00180730.1IPI: IPI00180730.1
Elongation factor 1-alphaElongation factor 1-alpha
178.55178.55
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00329441.1IPI: IPI00329441.1
ISY1;RAB43 Ras-related protein Rab-43ISY1; RAB43 Ras-related protein Rab-43
177.34177.34
1.00 1.00
--
0.90 0.90
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00736860.2IPI: IPI00736860.2
ELK2, member of ETS oncogene family, pseudogene 1ELK2, member of ETS oncogene family, pseudogene 1
177.21177.21
1.00 1.00
2.86 2.86
2.46 2.46
IPI:IPI00645370.1IPI: IPI00645370.1
VPS4B 16 kDa proteinVPS4B 16 kDa protein
177.1177.1
1.00 1.00
0.13 0.13
0.88 0.88
IPI:IPI00002926.4IPI: IPI00002926.4
VPS37B Vacuolar protein sorting-associated protein 37BVPS37B Vacuolar protein sorting-associated protein 37B
176.93176.93
1.00 1.00
--
1.08 1.08
IPI:IPI00477489.1IPI: IPI00477489.1
RAB4B Isoform 1 of Ras-related protein Rab-4BRAB4B Isoform 1 of Ras-related protein Rab-4B
176.87176.87
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00012540.1IPI: IPI00012540.1
PROM1 Prominin-1 precursorPROM1 Prominin-1 precursor
176.63176.63
1.00 1.00
0.19 0.19
0.99 0.99
IPI:IPI00745705.2IPI: IPI00745705.2
LOC730924 similar to Pancreatic alpha-amylase precursorLOC730924 similar to Pancreatic alpha-amylase precursor
175.5175.5
1.00 1.00
--
2.46 2.46
IPI:IPI00788782.1IPI: IPI00788782.1
ATP1A3 Cation-transporting ATPaseATP1A3 Cation-transporting ATPase
175.36175.36
1.00 1.00
1.09 1.09
--
IPI:IPI00302840.2IPI: IPI00302840.2
ATP1A3 Sodium/potassium-transporting ATPase subunit alpha-3ATP1A3 Sodium / potassium-transporting ATPase subunit alpha-3
175.36175.36
1.00 1.00
0.03 0.03
--
IPI:IPI00430842.3IPI: IPI00430842.3
IGHA1 IGHA1 proteinIGHA1 IGHA1 protein
175.11175.11
1.00 1.00
0.97 0.97
1.45 1.45
IPI:IPI00798247.1IPI: IPI00798247.1
HSPA8 20 kDa proteinHSPA8 20 kDa protein
174.84174.84
1.00 1.00
--
1.45 1.45
IPI:IPI00060801.1IPI: IPI00060801.1
RAB39B Ras-related protein Rab-39BRAB39B Ras-related protein Rab-39B
173.59173.59
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00014424.1IPI: IPI00014424.1
EEF1A2 Elongation factor 1-alpha 2EEF1A2 Elongation factor 1-alpha 2
172.45172.45
1.00 1.00
--
4.10 4.10
IPI:IPI00844239.1IPI: IPI00844239.1
Immunoblobulin G1 Fab heavy chain variable region (Fragment)Immunoblobulin G1 Fab heavy chain variable region (Fragment)
172.25172.25
--
1.00 1.00
1.05 1.05
IPI:IPI00218918.5IPI: IPI00218918.5
ANXA1 Annexin A1ANXA1 Annexin A1
170.07170.07
NormalNormal
Normal_highNormal_high
--
--
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00246975.8IPI: IPI00246975.8
GSTM3 Glutathione S-transferase Mu 3GSTM3 Glutathione S-transferase Mu 3
160.98160.98
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00015148.3IPI: IPI00015148.3
RAP1B Ras-related protein Rap-1b precursorRAP1B Ras-related protein Rap-1b precursor
159.81159.81
1.00 1.00
0.36 0.36
1.32 1.32
IPI:IPI00845493.1IPI: IPI00845493.1
Brain type mu-glutathione S-transferaseBrain type mu-glutathione S-transferase
159.72159.72
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00872974.1IPI: IPI00872974.1
GSTA2 Glutathione S-transferaseGSTA2 Glutathione S-transferase
158.01158.01
1.00 1.00
--
0.77 0.77
IPI:IPI00169383.3IPI: IPI00169383.3
PGK1 Phosphoglycerate kinase 1PGK1 Phosphoglycerate kinase 1
157.34157.34
1.00 1.00
--
0.55 0.55
IPI:IPI00005719.1IPI: IPI00005719.1
RAB1A Isoform 1 of Ras-related protein Rab-1ARAB1A Isoform 1 of Ras-related protein Rab-1A
156.04156.04
1.00 1.00
--
0.70 0.70
IPI:IPI00787396.1IPI: IPI00787396.1
GNAT3 similar to Guanine nucleotide-binding protein G(t), alpha-3 subunitGNAT3 similar to Guanine nucleotide-binding protein G (t), alpha-3 subunit
154.41154.41
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00797556.1IPI: IPI00797556.1
ANXA2 24 kDa proteinANXA2 24 kDa protein
153.1153.1
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00019345.1IPI: IPI00019345.1
RAP1A Ras-related protein Rap-1A precursorRAP1A Ras-related protein Rap-1A precursor
152.92152.92
1.00 1.00
0.36 0.36
1.35 1.35
IPI:IPI00796467.1IPI: IPI00796467.1
TF 11 kDa proteinTF 11 kDa protein
152.91152.91
--
1.00 1.00
0.44 0.44
IPI:IPI00023510.1IPI: IPI00023510.1
RAB5A Ras-related protein Rab-5ARAB5A Ras-related protein Rab-5A
152.53152.53
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00657682.2IPI: IPI00657682.2
GSTA1 Glutathione S-transferase A1GSTA1 Glutathione S-transferase A1
151.68151.68
1.00 1.00
--
0.71 0.71
IPI:IPI00783287.1IPI: IPI00783287.1
Immunglobulin heavy chain variable region (Fragment)Immunglobulin heavy chain variable region (Fragment)
150.22150.22
1.00 1.00
1.06 1.06
--
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00022977.1IPI: IPI00022977.1
CKB Creatine kinase B-typeCKB Creatine kinase B-type
407.27407.27
1.00 1.00
--
0.73 0.73
IPI:IPI00219018.7IPI: IPI00219018.7
GAPDH Glyceraldehyde-3-phosphate dehydrogenaseGAPDH Glyceraldehyde-3-phosphate dehydrogenase
407.22407.22
1.00 1.00
--
0.84 0.84
IPI:IPI00472610.2IPI: IPI00472610.2
IGHM IGHM proteinIGHM IGHM protein
406.9406.9
1.00 1.00
5.26 5.26
4.85 4.85
IPI:IPI00411356.5IPI: IPI00411356.5
VPS4A Vacuolar protein sorting-associating protein 4AVPS4A Vacuolar protein sorting-associating protein 4A
406.68406.68
1.00 1.00
--
0.83 0.83
IPI:IPI00440160.2IPI: IPI00440160.2
UEVLD Isoform 3 of Ubiquitin-conjugating enzyme E2 variant 3UEVLD Isoform 3 of Ubiquitin-conjugating enzyme E2 variant 3
406.44406.44
1.00 1.00
--
1.00 1.00
IPI:IPI00154742.6IPI: IPI00154742.6
IGL@ IGL@ proteinIGL @ IGL @ protein
406.41406.41
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00021447.1IPI: IPI00021447.1
AMY2B Alpha-amylase 2B precursorAMY2B Alpha-amylase 2B precursor
405.88405.88
1.00 1.00
--
3.71 3.71
IPI:IPI00784519.1IPI: IPI00784519.1
Putative uncharacterized proteinPutative uncharacterized protein
405.02405.02
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00450309.1IPI: IPI00450309.1
IGL@ IGL@ proteinIGL @ IGL @ protein
404.64404.64
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00796167.1IPI: IPI00796167.1
IGL@ IGL@ proteinIGL @ IGL @ protein
404.12404.12
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00217269.3IPI: IPI00217269.3
GNAT2 Guanine nucleotide-binding protein G(t) subunit alpha-2GNAT2 Guanine nucleotide-binding protein G (t) subunit alpha-2
403.37403.37
1.00 1.00
--
1.13 1.13
IPI:IPI00032328.2IPI: IPI00032328.2
KNG1 Isoform HMW of Kininogen-1 precursorKNG1 Isoform HMW of Kininogen-1 precursor
401.91401.91
1.00 1.00
--
0.78 0.78
IPI:IPI00003348.3IPI: IPI00003348.3
GNB2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2GNB2 Guanine nucleotide-binding protein G (I) / G (S) / G (T) subunit beta-2
398.88398.88
1.00 1.00
--
1.17 1.17
IPI:IPI00000885.1IPI: IPI00000885.1
FRK Tyrosine-protein kinase FRKFRK Tyrosine-protein kinase FRK
398.32398.32
1.00 1.00
--
0.73 0.73
IPI:IPI00785196.1IPI: IPI00785196.1
Putative uncharacterized proteinPutative uncharacterized protein
398.06398.06
IgANIgAN
--
IgAN_highIgAN_high
--
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00657796.1IPI: IPI00657796.1
CD9 Uncharacterized protein CD9CD9 Uncharacterized protein CD9
204.5204.5
1.00 1.00
--
1.90 1.90
IPI:IPI00063827.1IPI: IPI00063827.1
ABHD14B Isoform 1 of Abhydrolase domain-containing protein 14BABHD14B Isoform 1 of Abhydrolase domain-containing protein 14B
204.45204.45
1.00 1.00
--
0.73 0.73
IPI:IPI00644936.1IPI: IPI00644936.1
GNAS Isoform 3 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortGNAS Isoform 3 of Guanine nucleotide-binding protein G (s) subunit alpha isoforms short
204.01204.01
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00024282.1IPI: IPI00024282.1
RAB8B Ras-related protein Rab-8BRAB8B Ras-related protein Rab-8B
203.65203.65
1.00 1.00
--
0.69 0.69
IPI:IPI00514530.4IPI: IPI00514530.4
ACTA1 Uncharacterized protein ACTA1ACTA1 Uncharacterized protein ACTA1
202.61202.61
1.00 1.00
0.09 0.09
1.25 1.25
IPI:IPI00480056.7IPI: IPI00480056.7
RAB4A Uncharacterized protein RAB4ARAB4A Uncharacterized protein RAB4A
202.54202.54
1.00 1.00
--
1.07 1.07
IPI:IPI00103065.4IPI: IPI00103065.4
MITD1 MIT domain-containing protein 1MITD1 MIT domain-containing protein 1
201.63201.63
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00061114.1IPI: IPI00061114.1
RAB3C Ras-related protein Rab-3CRAB3C Ras-related protein Rab-3C
200.34200.34
1.00 1.00
--
0.91 0.91
IPI:IPI00871276.1IPI: IPI00871276.1
RAB3C 26 kDa proteinRAB3C 26 kDa protein
200.34200.34
1.00 1.00
--
0.90 0.90
IPI:IPI00219835.1IPI: IPI00219835.1
GNAS Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms shortGNAS Isoform Gnas-2 of Guanine nucleotide-binding protein G (s) subunit alpha isoforms short
200.12200.12
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00641459.3IPI: IPI00641459.3
EEF1A1 Similar to Elongation factor 1 alphaEEF1A1 Similar to Elongation factor 1 alpha
199.31199.31
1.00 1.00
--
0.43 0.43
IPI:IPI00377081.1IPI: IPI00377081.1
STOM stomatin isoform bSTOM stomatin isoform b
199.14199.14
1.00 1.00
--
0.68 0.68
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00745660.2IPI: IPI00745660.2
IGL@ IGL@ proteinIGL @ IGL @ protein
302.18302.18
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00294713.4IPI: IPI00294713.4
MASP2 Isoform 1 of Mannan-binding lectin serine protease 2 precursorMASP2 Isoform 1 of Mannan-binding lectin serine protease 2 precursor
300.93300.93
1.00 1.00
--
1.49 1.49
IPI:IPI00022255.1IPI: IPI00022255.1
OLFM4 Olfactomedin-4 precursorOLFM4 Olfactomedin-4 precursor
300.3300.3
1.00 1.00
0.07 0.07
1.86 1.86
IPI:IPI00784627.1IPI: IPI00784627.1
Putative Uncharacterized proteinPutative Uncharacterized protein
300.12300.12
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00784713.1IPI: IPI00784713.1
Putative Uncharacterized proteinPutative Uncharacterized protein
300.12300.12
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00871793.1IPI: IPI00871793.1
MASP2 77 kDa proteinMASP2 77 kDa protein
299.96299.96
1.00 1.00
--
2.69 2.69
IPI:IPI00790490.1IPI: IPI00790490.1
GPRC5C 48 kDa proteinGPRC5C 48 kDa protein
295.74295.74
1.00 1.00
0.12 0.12
1.39 1.39
IPI:IPI00099883.4IPI: IPI00099883.4
GPRC5C G-protein coupled receptor family C group 5 member C precursorGPRC5C G-protein coupled receptor family C group 5 member C precursor
295.74295.74
1.00 1.00
0.12 0.12
1.36 1.36
IPI:IPI00291262.3IPI: IPI00291262.3
CLU Clusterin precursorCLU Clusterin precursor
295.66295.66
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00795633.1IPI: IPI00795633.1
CLU CLUCLU CLU
295.66295.66
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00789107.1IPI: IPI00789107.1
RPS27A;UBC;UBB 30 kDa proteinRPS27A; UBC; UBB 30 kDa protein
294.28294.28
1.00 1.00
0.19 0.19
0.83 0.83
IPI:IPI00784983.1IPI: IPI00784983.1
Putative Uncharacterized proteinPutative Uncharacterized protein
293.91293.91
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00026268.3IPI: IPI00026268.3
GNB1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1GNB1 Guanine nucleotide-binding protein G (I) / G (S) / G (T) subunit beta-1
292.14292.14
1.00 1.00
--
1.14 1.14
IPI:IPI00791613.1IPI: IPI00791613.1
TUBA8 52 kDa proteinTUBA8 52 kDa protein
291.34291.34
NormalNormal
Normal_highNormal_high
--
--
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00465380.6IPI: IPI00465380.6
GNAS Uncharacterized protein GNAS (Fragment)GNAS Uncharacterized protein GNAS (Fragment)
168.01168.01
1.00 1.00
--
0.69 0.69
IPI:IPI00797951.1IPI: IPI00797951.1
HSPA8 16 kDa proteinHSPA8 16 kDa protein
166.78166.78
1.00 1.00
--
1.43 1.43
IPI:IPI00788210.1IPI: IPI00788210.1
LOC728590 similar to ribosomal protein S27aLOC728590 similar to ribosomal protein S27a
166.62166.62
1.00 1.00
--
0.59 0.59
IPI:IPI00792992.1IPI: IPI00792992.1
RAB37 22 kDa proteinRAB37 22 kDa protein
166.16166.16
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00550181.2IPI: IPI00550181.2
CHMP2B Charged multivesicular body protein 2bCHMP2B Charged multivesicular body protein 2b
166.07166.07
1.00 1.00
--
1.28 1.28
IPI:IPI00023504.1IPI: IPI00023504.1
RAB3A Ras-related protein Rab-3ARAB3A Ras-related protein Rab-3A
165.51165.51
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00556414.1IPI: IPI00556414.1
Mel transforming oncogene variant (Fragment)Mel transforming oncogene variant (Fragment)
165.16165.16
1.00 1.00
--
0.75 0.75
IPI:IPI00412525.1IPI: IPI00412525.1
hCG_1757335;RAP1B Uncharacterized protein ENSP00000345280hCG_1757335; RAP1B Uncharacterized protein ENSP00000345280
164.4164.4
1.00 1.00
0.41 0.41
1.28 1.28
IPI:IPI00220301.5IPI: IPI00220301.5
PRDX6 Peroxiredoxin-6PRDX6 Peroxiredoxin-6
163.55163.55
1.00 1.00
--
0.58 0.58
IPI:IPI00477090.6IPI: IPI00477090.6
IGHM IGHM proteinIGHM IGHM protein
163.16163.16
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00873902.1IPI: IPI00873902.1
RAB1A 23 kDa proteinRAB1A 23 kDa protein
162.44162.44
1.00 1.00
--
0.67 0.67
IPI:IPI00187143.1IPI: IPI00187143.1
RAB4B Isoform 2 of Ras-related protein Rab-4BRAB4B Isoform 2 of Ras-related protein Rab-4B
162.37162.37
TGBMTGBM
--
--
TGBM_highTGBM_high
IPI:IPI00854833.1IPI: IPI00854833.1
RAP1A Uncharacterized protein RAP1ARAP1A Uncharacterized protein RAP1A
161.39161.39
1.00 1.00
--
1.63 1.63
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00797482.1IPI: IPI00797482.1
RPS27A;UBC;UBB CDNA FLJ32377 fis, clone SKMUS1000014, highly similar to Polyubiquitin 9RPS27A; UBC; UBB CDNA FLJ32377 fis, clone SKMUS1000014, highly similar to Polyubiquitin 9
386.38386.38
1.00 1.00
0.19 0.19
0.83 0.83
IPI:IPI00788737.1IPI: IPI00788737.1
GAPDH 39 kDa proteinGAPDH 39 kDa protein
384.98384.98
1.00 1.00
--
0.89 0.89
IPI:IPI00643007.1IPI: IPI00643007.1
KIAA0174 Isoform 3 of Uncharacterized protein KIAA0174KIAA0174 Isoform 3 of Uncharacterized protein KIAA0174
383.66383.66
1.00 1.00
0.17 0.17
1.67 1.67
IPI:IPI00797523.1IPI: IPI00797523.1
HSPA8 20 kDa proteinHSPA8 20 kDa protein
383.66383.66
1.00 1.00
--
0.71 0.71
IPI:IPI00397808.3IPI: IPI00397808.3
LOC388720 similar to ubiquitin and ribosomal protein S27a precursorLOC388720 similar to ubiquitin and ribosomal protein S27a precursor
382.66382.66
1.00 1.00
--
0.81 0.81
IPI:IPI00215894.1IPI: IPI00215894.1
KNG1 Isoform LMW of Kininogen-1 precursorKNG1 Isoform LMW of Kininogen-1 precursor
382.13382.13
1.00 1.00
--
0.81 0.81
IPI:IPI00797833.3IPI: IPI00797833.3
KNG1 Kininogen 1KNG1 Kininogen 1
382.13382.13
1.00 1.00
--
0.78 0.78
IPI:IPI00796633.1IPI: IPI00796633.1
TPI1 22 kDa proteinTPI1 22 kDa protein
381.1381.1
1.00 1.00
--
0.94 0.94
IPI:IPI00016339.4IPI: IPI00016339.4
RAB5C Ras-related protein Rab-5CRAB5C Ras-related protein Rab-5C
380.62380.62
1.00 1.00
--
1.12 1.12
IPI:IPI00017160.3IPI: IPI00017160.3
VTA1 Vacuolar protein sorting-associated protein VTA1 homologVTA1 Vacuolar protein sorting-associated protein VTA1 homolog
378.92378.92
1.00 1.00
--
0.79 0.79
IPI:IPI00642632.1IPI: IPI00642632.1
C7 proteinC7 protein
376.2376.2
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00300786.1IPI: IPI00300786.1
AMY1A;AMY1C;AMY2A;AMY1B Alpha-amylase 1 precursorAMY1A; AMY1C; AMY2A; AMY1B Alpha-amylase 1 precursor
375.66375.66
1.00 1.00
--
2.80 2.80
IPI:IPI00793930.1IPI: IPI00793930.1
TUBA1B TUBA1B proteinTUBA1B TUBA1B protein
375.65375.65
1.00 1.00
--
1.01 1.01
IPI:IPI00816314.1IPI: IPI00816314.1
IGHM Putative uncharacterized protein DKFZp686I15196IGHM Putative uncharacterized protein DKFZp686I15196
375.23375.23
1.00 1.00
5.00 5.00
4.66 4.66
IPI:IPI00018901.2IPI: IPI00018901.2
GGT1 Isoform 1 of Gamma-glutamyltranspeptidase 1 precursorGGT1 Isoform 1 of Gamma-glutamyltranspeptidase 1 precursor
369.2369.2
1.00 1.00
--
0.62 0.62
Accession No.Accession No.
DescriptionDescription
ScoreScore
UniqueUnique
NormalNormal
IgANIgAN
TGBMTGBM
IPI:IPI00396108.2IPI: IPI00396108.2
GPRC5C G protein-coupled receptor family C, group 5, member C isoform aGPRC5C G protein-coupled receptor family C, group 5, member C isoform a
321.89321.89
1.00 1.00
0.11 0.11
1.39 1.39
IPI:IPI00785164.1IPI: IPI00785164.1
Putative Uncharacterized proteinPutative Uncharacterized protein
321.63321.63
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00788054.1IPI: IPI00788054.1
GGT2 similar to Gamma-glutamyltranspeptidase 1 precursor (Gamma-glutamyltransferase 1) (CD224 antigen) isoform 2GGT2 similar to Gamma-glutamyltranspeptidase 1 precursor (Gamma-glutamyltransferase 1) (CD224 antigen) isoform 2
321.37321.37
1.00 1.00
--
0.58 0.58
IPI:IPI00607626.1IPI: IPI00607626.1
FLOT1 Flotillin 1FLOT1 Flotillin 1
321.32321.32
NormalNormal
Normal_highNormal_high
--
--
IPI:IPI00785084.1IPI: IPI00785084.1
Immunoglobulin heavy variable 4-31Immunoglobulin heavy variable 4-31
321.29321.29
--
1.00 1.00
0.91 0.91
IPI:IPI00301277.1IPI: IPI00301277.1
HSPA1L Heat shock 70 kDa protein 1LHSPA1L Heat shock 70 kDa protein 1L
319.05319.05
1.00 1.00
--
1.36 1.36
IPI:IPI00643152.1IPI: IPI00643152.1
HSPA1L Heat shock 70kDa protein 1-like variantHSPA1L Heat shock 70kDa protein 1-like variant
319.05319.05
1.00 1.00
--
1.20 1.20
IPI:IPI00218488.3IPI: IPI00218488.3
GNAT1 Guanine nucleotide-binding protein G(t) subunit alpha-1GNAT1 Guanine nucleotide-binding protein G (t) subunit alpha-1
318.67318.67
1.00 1.00
--
1.13 1.13
IPI:IPI00815938.1IPI: IPI00815938.1
IGLV3-21 IGLV3-21 proteinIGLV3-21 IGLV3-21 protein
316.8316.8
IgANIgAN
--
IgAN_highIgAN_high
--
IPI:IPI00745360.1IPI: IPI00745360.1
GGT2 Uncharacterized protein ENSP00000255845GGT2 Uncharacterized protein ENSP00000255845
316.54316.54
1.00 1.00
--
0.64 0.64
IPI:IPI00829711.1IPI: IPI00829711.1
IGHA2 Uncharacterized protein IGHA2 (Fragment)IGHA2 Uncharacterized protein IGHA2 (Fragment)
314.86314.86
1.00 1.00
0.84 0.84
1.13 1.13
IPI:IPI00793729.1IPI: IPI00793729.1
RPS27A;UBC;UBB UBC proteinRPS27A; UBC; UBB UBC protein
314.72314.72
1.00 1.00
0.19 0.19
0.82 0.82
IPI:IPI00248911.7IPI: IPI00248911.7
GNAT3 guanine nucleotide binding protein, alpha transducing 3GNAT3 guanine nucleotide binding protein, alpha transducing 3
313.56313.56
1.00 1.00
--
1.12 1.12
IPI:IPI00004901.5IPI: IPI00004901.5
GPRC5C CDNA FLJ20242 fis, clone COLF6369GPRC5C CDNA FLJ20242 fis, clone COLF6369
313.55313.55
1.00 1.00
0.14 0.14
1.32 1.32
본 발명에서 IgA 신증과 TGBM 신증 환자에서 증감을 보인 단백질들 중에서 Aminopeptidase N, Vasorin precursor, Ceruloplasmin, Alpha-1-antitrypsin을 선택하여 웨스턴 블랏 실시한 결과는 도 3(Normal: 정상인, IgAN: IgA 신증 환자, TGBM: TGBM 신증 환자)에 나타내었다.In the present invention, the results of Western blot selection of Aminopeptidase N, Vasorin precursor, Ceruloplasmin, and Alpha-1-antitrypsin among the proteins sensitized in IgA nephropathy and TGBM nephropathy are shown in FIG. 3 (Normal: normal, IgAN: TGBM: patients with TGBM nephropathy).