KR100911741B1 - Multi-wavelength optical parametric oscillation laser source apparatus and cancer detection system using thereof - Google Patents

Abstract

The present invention discloses a multi-wavelength OPO light source device and a cancer diagnosis system using the same. Cancer diagnosis system according to an embodiment of the present invention is an OPO laser that outputs a preset multi-wavelength excitation light source based on an optical parametric oscillation (OPO) method, tissue to receive the multi-wavelength excitation light source to diagnose cancer And a light probe for receiving a fluorescence signal for the excitation light source from the tissue, a spectroscope for spectroscopy of the fluorescence signal received with the optical probe, and a signal spectroscopically detected by the spectroscope. And an optical detector for outputting the detected signal for each wavelength to a display unit displaying the spectral spectrum of the fluorescent signal.

Optical parametric oscillation (OPO), oral, cancer, diagnostic, multi-wavelength

Description

MULTI-WAVELENGTH OPTICAL PARAMETRIC OSCILLATION LASER SOURCE APPARATUS AND CANCER DETECTION SYSTEM USING THEREOF}

The present invention relates to cancer diagnosis, and in particular, multi-wavelength OPO that can spectroscopically fluoresce the fluorescence signal of a tissue for a multi-wavelength excitation light source output from an OPO laser and diagnose cancer through the spectral spectrum of the fluorescence signal. A light source device and a cancer diagnosis system using the same.

When phosphors in a human body change due to cancerous tissue in a normal state, there is an optical cancer diagnosis method that can measure the biochemical and optical changes of the phosphors and diagnose the change.

The optical cancer diagnosis method uses a technique for diagnosing only a single region using a single wavelength and a difference in the wavelength of the laser light source irradiated according to the components of the tissue and the components of the phosphors present inside the tissue. There is a cancer diagnosis technique using spectroscopy.

The fluorescence spectroscopy system using a single wavelength ignores individual characteristics of the phosphors and diagnoses the occurrence of chemical change at the tissue level as a whole by the difference in the fluorescence spectrum. Problems that are difficult to interpret arise.

The technology to solve the problem of the fluorescence spectroscopy system using a single wavelength is a cancer diagnosis technology using multi-wavelength fluorescence spectroscopy.

Conventional fluorescence spectroscopy system using multi-wavelength uses a dye laser made by randomly selecting a wavelength and putting up to 10 dyes generating different wavelengths in each qubit.

However, since the fluorescence spectroscopy system using the multi-wavelength uses a dye laser whose wavelength is variable for the purpose of analyzing and diagnosing the intrinsic wavelength characteristics of the phosphors, the diagnosis is performed to satisfy the characteristics of the phosphors depending on the part of the human body to be measured. When the target is determined, there is a problem in that the individual fluorescence spectroscopy characteristics of the phosphors present in the biological tissues for the diagnosis site are determined and the light source corresponding to the excitation wavelength is frequently made by using the pigment.

In addition, due to the short life of the pigment, it is necessary to make the pigment three to four times a year, and the materials such as 'rhodamine pigment', which is the raw material of the dye laser, are carcinogenic, so be careful in the manufacturing stage. There is also a need.

In addition, the wavelength should be changed in real time according to what kind of phosphors are present inside the tissue. The pigment laser is qubited after making a pigment that cannot be changed in real time and the expected wavelength can be generated for cancer diagnosis. Change the wavelength while changing the. Therefore, when the diagnosis site is changed or the target phosphors are changed, the produced pigment becomes useless and there is a problem about the limit of wavelength variability in which a new pigment should be made.

That is, the conventional fluorescence spectroscopy diagnostic system using a multi-wavelength dye laser has the problems of equipment manufacturing, maintenance risk, equipment life and limit of wavelength variability.

An object of the present invention is to solve the above problems, by performing a cancer diagnosis using an OPO laser outputting an excitation light source of multiple wavelengths, it is possible to selectively use a desired wavelength in a wide range of wavelength range To provide a multi-wavelength OPO light source device and cancer diagnosis system using the same.

Another object of the present invention is to use an OPO laser that outputs a multi-wavelength excitation light source, a multi-wavelength OPO light source device that can solve the manufacturing difficulties caused by the raw material of the dye laser when producing a dye laser and a cancer diagnosis system using the same To provide.

Another object of the present invention is to use a multi-wavelength OPO light source device that can change the wavelength change in real time compared to the dye laser by using an OPO laser that can selectively use a desired wavelength in a wide range of wavelength range and cancer diagnosis system using the same To provide.

Still another object of the present invention is to provide a multi-wavelength OPO light source device and a cancer diagnosis system using the same, which can increase the life of the equipment by performing the multi-wavelength variable using a semi-permanent crystal solid pigment contained in the OPO laser. .

Still another object of the present invention is to provide a multi-wavelength OPO light source device and a cancer diagnosis system using the same, by using an OPO laser having a stable output energy intensity, thereby increasing the reliability of a cancer diagnosis result.

In order to achieve the above object, a cancer diagnosis system according to an aspect of the present invention receives an OPO laser that outputs a preset multi-wavelength excitation light source based on an optical parametric oscillation (OPO) scheme, and receives the multi-wavelength excitation light source. An optical probe for transmitting cancer to a tissue to be diagnosed and receiving a fluorescent signal for the excitation light source from the tissue, a spectroscope for spectroscopically spectroscopy the fluorescent signal received with the optical probe, and a wavelength by the spectroscope And a photo detector for detecting a signal spectroscopically and outputting the signal spectroscopically detected to each wavelength to a display unit displaying a spectroscopic spectrum of the fluorescent signal.

In this case, the OPO laser may output the excitation light source having wavelengths of 287, 290, 325, 340, 350, 360, 390, 440, 450 and 470 [nm].

In this case, the cancer diagnosis system further comprises a light generating unit for generating light for measuring the reflection spectroscopy of the tissue, the light probe is the excitation light source output from the OPO laser and the light generated from the light generating unit. The tissue may be delivered to the tissue, and the fluorescence signal and the reflection signal of the tissue may be received and output to the spectrometer.

In this case, the cancer diagnosis system is a lens for the first light transmission unit for selectively transmitting the wavelength of the excitation light source output from the OPO laser and the light generated from the light generation unit and the light output from the first light transmission unit It may further include a second light transmission unit for transmitting to the optical probe through.

In order to achieve the above object, a multi-wavelength OPO light source device according to an aspect of the present invention is an OPO laser for outputting a preset multi-wavelength excitation light source based on the OPO method, collecting the excitation light source output from the OPO laser And a light transmission unit for selectively outputting a cylindrical lens and a wavelength of the excitation light source collected through the cylindrical lens.

As described above in detail, the present invention has an effect of selectively using a desired wavelength in a wide range of wavelengths by performing cancer diagnosis using an OPO laser that outputs a multi-wavelength excitation light source.

The present invention has an effect that can solve the difficulty of the production caused by the raw material of the dye laser when producing a dye laser by using an OPO laser that outputs a multi-wavelength excitation light source.

The present invention has the effect of changing the wavelength variability in real time compared to the dye laser by using an OPO laser that can selectively use a desired wavelength in a wide range of wavelength range.

The present invention has the effect of extending the life of the equipment by performing a multi-wavelength variable using a semi-permanent crystal solid pigment contained in the OPO laser.

Other objects and features of the present invention in addition to the above object will be apparent from the description of the embodiments with reference to the accompanying drawings.

Hereinafter, a multi-wavelength OPO light source device and a cancer diagnosis system using the same according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 3.

OPO (optical parametric oscillation) is a light source that changes the wavelength by using a nonlinear optical crystal (non-linear optical effect) instead of a pigment, and rotates the angle of the non-linear single crystal to change the wavelength of the output light.

The present invention outputs a multi-wavelength excitation light source for cancer diagnosis using an OPO laser having the characteristics as described above, and diagnoses whether cancer is detected through a fluorescent signal for phosphors using the output multi-wavelength excitation light source. Suggest a system that can.

1 is a block diagram of a cancer diagnosis system using a multi-wavelength OPO light source device according to an embodiment of the present invention.

Referring to FIG. 1, a cancer diagnosis system using a multi-wavelength OPO light source device includes an OPO laser 101, a first lens 105, a first light transmission unit 106, a filter unit 107, and a light generation unit 104. ), A second light transmission unit 108, a second lens 109, an optical probe 111, a spectrometer 112, a light detector 113, a controller 110, and a display unit 114.

The controller 110 controls the pulse length of the OPO laser 101 and the shutter speed of the photo detector 113, and controls the pulses of the OPO laser 101 and the shutter speed of the photo detector 113 to be synchronized.

At this time, the pulse length of the OPO laser 101 may be about 5 [ns].

The OPO laser 101 outputs an excitation light source of multiple wavelengths under the control of the controller 110.

In this case, the OPO laser 101 can vary the wavelength of 250 ~ 2400 [nm], and can output an excitation light source having 10 different wavelengths for cancer diagnosis.

In this case, the OPO laser 101 may output an excitation light source having wavelengths of 287, 290, 325, 340, 350, 360, 390, 440, 450, and 470 [nm] to diagnose oral cancer.

The OPO laser 101 includes a pulse laser 102 and a crystalline solid pigment 103.

The pulse laser 102 is an excitation light source of the crystalline solid dye 103 and may be an Nd-Yag pulse laser having a wavelength of 355 [nm].

The crystal solid dye 103 outputs an excitation light source having various wavelengths according to the incident angle of the excitation light source output from the pulse laser 102. For example, in the diagnosis of oral cancer, the crystal solid pigment 103 has 10 wavelengths in the region of 250 to 600 nm, that is, tryptophan, collagen, elastin, and nicotinamide adenine dinucleotide hydrogen (NADH). It outputs an excitation light source having excitation wavelengths for phosphors of FAD (flavin adenine dinucleotide), protoporphyrin IX, riboflavin, lipo-pigments and pyridoxine.

In this case, the crystal solid pigment 103 may be a BBO (Beta-Barium Borate) crystal or LBO (Lithium Borta) crystal.

The first lens 105 is a cylindrical lens, and the excitation light source output from the OPO laser 101 is incident to the first light transmission unit 106.

The first light transmitting unit 106 serves as a beam splitter for selectively irradiating the optical probe 111 with the multi-wavelength OPO light source incident from the first lens 105.

The filter unit 107 is a band pass filter and selects and outputs only the light source output from the first light transmission unit 106. That is, the filter unit 107 selects and outputs only the OPO light source from which the ambient light source is removed.

The light generator 104 generates light for measuring the reflection spectroscopy of the tissue to be diagnosed with cancer.

In this case, the light generating unit 104 may be a xenon lamp having a wavelength range of 200 to 1300 [nm], and the reflection spectral value measured using the light generated from the light generating unit 104 may be a tissue for cancer diagnosis. It can be used when analyzing the fluorescence spectral value of.

The second light transmission unit 108 transmits the light source output through the filter unit 107 and the light source generated from the light generation unit 104 to the light probe 111 through the second lens 109.

That is, the second light transmission unit 108 transmits the OPO light source having the excitation wavelength of the phosphors included in the tissue and the light for measuring the reflection spectral value to the light probe 111.

The optical probe 111 transmits the light source of the OPO and the light generator 104 that are transmitted through the second lens 109 to the tissue to diagnose cancer, and transmits the fluorescence signal generated from the tissue to the spectroscope 112. To pass.

In this case, the optical probe 111 is composed of one optical probe at the center and six optical probes at the periphery thereof, and the optical probe at the center is a light source and a light generator 104 of the OPO transmitted through the second lens 109. The light source of) is transmitted to the tissue, and the six light probes located in the vicinity transmit the fluorescence signal generated from the tissue to the spectrometer 112.

The spectroscope 112 spectroscopy the fluorescent signal received by the optical probe 111 for each wavelength.

That is, the spectroscope 112 spectroscopy the fluorescent signal received by the optical probe 111 for each wavelength so as to diagnose cancer of the tissue.

In this case, the spectroscope 112 may include two gratings of 150 [groove / mm].

The photo detector 113 detects the intensity of the signal spectroscopically by the spectrometer 112 for each wavelength.

At this time, the photo detector 113 is an ICCD (intensified charged coupled detector), the shutter is adjusted to a gated time (gated time) of 5 [ns] through the control by the controller 110 and the pulse of the OPO that is the excitation light source Are motivated.

The display unit 114 displays a fluorescence spectral spectrum for the tissue based on the intensity of the signal spectroscopically detected by the photodetector 113 and controls the driving of the system. That is, the display unit 114 displays the spectroscopic signals of the fluorescent substances of the tissue in a spectrum, thereby diagnosing cancer of the tissue.

The operation of the cancer diagnosis system using the multi-wavelength OPO light source device according to the present invention having the configuration as described above will be further described with reference to FIG. 2.

2 is an exemplary view showing wavelength values of phosphors in living tissue for diagnosing oral cancer.

As can be seen in Figure 2, the cancer diagnosis system using a multi-wavelength OPO light source device according to the present invention is a fluorescent substance inside tryptophan (tryptophan), collagen (collagen), elastin for oral cancer diagnosis using OPO laser excitation that can excite (elastin), nicotinamide adenine dinucleotide hydrogen (NADH), flavor adenine dinucleotide (FAD), protoporphyrin IX, riboflavin, lipo-pigments, and pyridoxine Output the wavelength.

That is, the OPO laser 101 is 287 [nm] which is an excitation wavelength of tryptophan, phosphors present in oral tissue, 290 [nm] which is an excitation wavelength of elastin, and 325 [excitation wavelength of NADH]. nm] and 350 [nm], 340 [nm] which is the excitation wavelength of pyridoxine, 360 [nm] which is the excitation wavelength of collagen, 390 [nm] which is the excitation wavelength of lipo-pigments, A multi-wavelength excitation light source with an excitation wavelength of 440 [nm] of protoporphyrin IX, 450 [nm] of an excitation wavelength of FAD and 470 [nm] of an excitation wavelength of riboflavin is output.

When the excitation light source output from the OPO laser 101 is transmitted to the oral living tissue through the light probe 111, each of the phosphors outputs an emission wavelength with respect to the excitation wavelength.

The optical probe 111 receives a fluorescent signal from a living tissue including a wavelength of emission of each phosphor, that is, wavelengths of 340, 348, 400, 405, 440, 462, 515, 520-535, 560 and 635 [nm]. do.

When the fluorescence signal including the divergence wavelength shown in FIG. 2 is spectrophotometrically by the spectroscope 112, the intensity of the signal spectroscopically detected by the photodetector 113 is detected and based on the detected divergence wavelength-specific intensity The display unit 114 displays the fluorescence spectral spectrum.

The fluorescence spectral spectrum displayed on the display unit 114 may indicate the intensity of the phosphors with respect to the emission wavelength and diagnose the oral cancer based on the intensity with respect to the emission wavelength.

3 is a block diagram of a multi-wavelength OPO light source device according to an embodiment of the present invention.

Referring to FIG. 3, the multi-wavelength OPO light source device includes an OPO laser 301, a lens 304, and a light transmission unit 305.

The OPO laser 301 outputs a multi-wavelength excitation light source for cancer diagnosis.

At this time, the OPO laser 301 is possible to vary the wavelength of 250 ~ 2400 [nm], and if you want to diagnose oral cancer 287, 290, 325, 340, 350, 360, The excitation light sources having multiple wavelengths of 390, 440, 450 and 470 [nm] can be output.

Here, the OPO laser 301 includes a pulse laser 302 and a crystalline solid dye 303, which is an excitation light source of the crystalline solid dye 303, having a wavelength of 355 [nm]. Yag pulse laser is used.

The crystal solid dye 303 outputs an excitation light source having various wavelengths according to the incident angle of the excitation light source output from the pulse laser 302. For example, when the oral cancer is to be diagnosed, the crystal solid pigment 303 outputs an excitation light source having ten excitation wavelengths shown in FIG. 2.

In this case, the crystalline solid pigment 303 may be a BBO crystal or an LBO crystal.

The lens 304 is a cylindrical lens and enters the excitation light source output from the OPO laser 301 into the light transmission unit 305.

The light transmitting unit 305 selectively outputs the multi-wavelength OPO light source incident from the lens 304.

Here, the wavelength region of the OPO laser 301 generated through the light transmission unit 205 can be a wavelength variable to 400 ~ 2400 [nm] region when the crystal solid pigment 303 is a BBO crystal, the second harmonic ( second harmonic) wavelength can be generated in the range of 200 ~ 420 [nm].

The multi-wavelength OPO light source device and the cancer diagnosis system using the same according to the present invention can be modified and applied in various forms within the scope of the technical idea of the present invention and are not limited to the above embodiments. In addition, the embodiments and drawings are merely for the purpose of describing the contents of the invention in detail, not intended to limit the scope of the technical idea of the invention, the present invention described above is common knowledge in the technical field to which the present invention belongs As those skilled in the art can have various substitutions, modifications, and changes without departing from the spirit of the present invention, it is not limited to the embodiments and the accompanying drawings. And should be judged to include equality.

1 is a block diagram of a cancer diagnosis system using a multi-wavelength OPO light source device according to an embodiment of the present invention.

2 is an exemplary view showing wavelength values of phosphors in living tissue for diagnosing oral cancer.

3 is a block diagram of a multi-wavelength OPO light source device according to an embodiment of the present invention.

<Description of Symbols for Main Parts of Drawings>

101,301: OPO laser

102,302: pulsed laser

103,303: Crystalline Solid Pigment

104: light generating unit

105: first lens

106: first light transmission unit

107: filter section

108: second light transmission unit

109: second lens

110: controller

111: light probe

112: spectrometer

113: light detector

114: display unit

304: lens

305: light transmission unit

Claims (10)

An OPO laser for outputting an excitation light source of a preset multi-wavelength based on an optical parametric oscillation (OPO) scheme; An optical probe receiving the multi-wavelength excitation light source and delivering the cancer to a tissue to be diagnosed, and receiving a fluorescent signal for the excitation light source from the tissue; A spectroscope for spectroscopy the fluorescence signal received by the optical probe for each wavelength; And An optical detector for detecting a signal spectroscopically detected by the spectrometer and outputting the signal spectroscopically detected for each wavelength to a display unit displaying a spectroscopic spectrum of the fluorescent signal Cancer diagnostic system comprising a. The method of claim 1, The OPO laser And an excitation light source having ten different wavelengths for oral cancer diagnosis. The method of claim 2, The OPO laser And outputting the excitation light sources having wavelengths of 287, 290, 325, 340, 350, 360, 390, 440, 450 and 470 [nm]. The method of claim 1, The OPO laser A pulse laser outputting a light source having a preset wavelength; And Crystal solid dye which outputs the excitation light source based on the light source output from the pulse laser Cancer diagnostic system comprising a. The method of claim 4, wherein The pulse laser is It is a Nd-Yag pulse laser which outputs the light source of wavelength 355 [nm], The cancer diagnostic system characterized by the above-mentioned. The method of claim 4, wherein The crystalline solid pigment is Cancer diagnosis system, characterized in that the BBO (Beta-Barium Borate) crystal or LBO (Lithium Borta) crystal. The method of claim 1, The cancer diagnosis system A light generating unit for generating light for measuring the reflection spectroscopy of the tissue More, The optical probe is The excitation light source output from the OPO laser and the light generated from the light generating unit delivers to the tissue, and receives the fluorescent signal and the reflection signal of the tissue and outputs to the spectroscope. The method of claim 7, wherein The cancer diagnosis system A first light transfer unit selectively transferring a wavelength of the excitation light source output from the OPO laser; And A second light transfer unit configured to transfer the light generated from the light generation unit and the light output from the first light transfer unit to the light probe through a lens; Cancer diagnosis system, characterized in that it further comprises. The method of claim 1, The cancer diagnosis system A controller for controlling the pulse length of the OPO laser and the shutter speed of the photo detector Cancer diagnosis system, characterized in that it further comprises. An OPO laser for outputting an excitation light source of a preset multi-wavelength based on the OPO method; A cylindrical lens collecting the excitation light source output from the OPO laser; And A light transmission unit selectively outputting a wavelength of the excitation light source collected through the cylindrical lens Multi-wavelength OPO light source device comprising a.

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