KR100882084B1 - Preparation method of a ginger extract using enzyme fermentation of honey - Google Patents
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- KR100882084B1 KR100882084B1 KR1020080004800A KR20080004800A KR100882084B1 KR 100882084 B1 KR100882084 B1 KR 100882084B1 KR 1020080004800 A KR1020080004800 A KR 1020080004800A KR 20080004800 A KR20080004800 A KR 20080004800A KR 100882084 B1 KR100882084 B1 KR 100882084B1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
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Abstract
Description
본 발명은 꿀의 효소발효를 이용한 신규한 생강추출액의 제조방법에 관한 것으로, 더욱 상세하게는 꿀의 효소발효에 의하여 생강의 매운맛, 쓴맛 등의 특유한 자극적인 맛이 제거될 뿐만 아니라 유효 성분 중 진저롤(gingerol)을 다량 함유하는 생강추출액의 제조 방법에 관한 것이다. The present invention relates to a method for producing a novel ginger extract using the enzyme fermentation of honey, and more specifically, by the enzyme fermentation of honey not only removes the pungent taste, such as the spicy, bitter taste of ginger, but also gingerbread in the active ingredient It relates to a method for producing a ginger extract containing a large amount (gingerol).
생강(生薑)은 특유의 향기와 매운맛 때문에 가정에서 식품원료인 향신료로 사용되어 왔으며, 특히 한방에서는 생강의 뿌리줄기를 말린 것을 건강(乾薑)이라 하여 위액분비촉진, 장관 연동작용 활성화, 소화촉진, 및 혈액순환 촉진을 위한 한약재로서 사용되고 있다. 특히, 생강의 약리 성분 중 진저롤이 이러한 역할을 하는 것으로 알려져 있으며, 대한약전에 의하면 진저롤의 함량이 0.4 중량% 이상인 건강(乾薑)은 한약재로 분류된다. Ginger (生薑) has been used as a food ingredient at home because of its unique aroma and spicy taste, especially in oriental medicine, dried root stem of ginger is called health (건강) to promote gastric secretion, activate the intestinal peristalsis, digestion It is used as an herbal medicine for promoting and promoting blood circulation. In particular, ginger ginger is known to play a role in the pharmacological component of ginger, according to the Korean Pharmacopoeia Gingerol content of more than 0.4% by weight is classified as a herbal medicine.
그러나, 상기와 같이 유용한 기능을 많이 가지는 생강은 매운맛, 쓴맛 등의 특유의 자극적인 맛으로 인해 식품에 직접 첨가하거나 착즙하여 첨가할 경우, 그 첨가량이 제한되는 문제가 있었다. However, ginger, which has many useful functions as described above, has a problem in that its amount is limited when it is added directly to a food or a juice is added due to a characteristic irritating taste such as spicy and bitter taste.
또한, 종래에는 생강의 약리 성분 중 진저롤을 추출하기 위하여 열탕 추출법이나 착즙법이 사용되고 있었으나, 열탕 추출법은 진저롤의 추출 수율이 극히 낮다는 문제가 있었고, 착즙법은 진저롤의 추출 수율은 높으나 착즙시에 생강 내의 전분도 함께 추출됨으로써 농축 공정시에 전분이 호화(糊化)되어 페이스트로 될 뿐만 아니라 농축액을 보관하게 되면 갈변 반응이 발생함은 물론 이취를 발생시키는 문제가 있었다.In addition, hot water extraction method or juice extraction method has been used to extract ginger roll among pharmacological components of ginger, but hot water extraction method has a problem that extraction yield of ginger roll is extremely low, and juice extraction method has high extraction yield of ginger roll, but at the time of juice Starch in ginger is also extracted, so that the starch is gelatinized during the concentration process to become a paste, and when the concentrate is stored, browning reactions occur as well as a bad smell.
본 발명은 상기와 같은 종래의 문제점을 해결하기 위한 것으로, 생강 고유의 효능은 지니고 있으나 매운맛, 쓴맛 등 특유의 자극적인 맛이 제거된 생강추출액의 제조방법을 제공하는 것을 목적을 한다. The present invention is to solve the conventional problems as described above, but has an inherent effect of ginger, but an object of the present invention is to provide a method for preparing a ginger extract liquid, such as spicy, bitter taste is removed.
또한, 본 발명은 생강의 유효 성분 중 진저롤(gingerol)을 0.4 중량% 이상 함유하는 생강추출액의 제조방법을 제공하는 것을 목적으로 한다. In addition, an object of the present invention is to provide a method for producing a ginger extract containing 0.4% by weight or more of ginger in the active ingredient of ginger.
상기 본 발명의 목적은, 수세정선된 생강 원료를 수분함량 20~30%에 이르도록 일광 또는 열풍 건조하는 단계; 상기 생강을 두께 1~4㎜로 얇게 써는 단계; 얇게 썬 상기 생강과 꿀을 1:1~2의 중량 비율로 혼합하는 단계; 및 상기 혼합물을 35~45℃에서 3~4일간 효소발효시키는 단계를 포함하는 것을 특징으로 하는 생강추출액의 제조방법을 통해 달성된다. An object of the present invention, the step of drying the washed raw ginger material to a moisture content of 20 to 30% to daylight or hot air; Writing the ginger thinly to a thickness of 1 to 4 mm; Mixing the sliced ginger and honey in a weight ratio of 1: 1 to 2; And the mixture is achieved through a method for producing a ginger extract, characterized in that it comprises the step of fermenting the enzyme at 35 ~ 45 ℃ for 3-4 days.
또한, 상기 본 발명의 목적은, 수세정선된 생강 원료를 수분함량 20~30%에 이르도록 일광 또는 열풍 건조하는 단계; 상기 생강을 두께 1~4㎜로 얇게 써는 단계; 수제정선된 인삼, 대추, 오미자, 계피, 및 복분자 중 적어도 어느 하나를 두께 1~4㎜로 얇게 써는 단계; 얇게 썬 상기 생강과, 상기 인삼, 대추, 오미자, 계피, 및 복분자 중 적어도 어느 하나와, 꿀을 1:0.5:1~2의 중량 비율로 혼합하는 단계; 및 상기 혼합물을 35~45℃에서 3~4일간 효소발효시키는 단계를 포함하는 것을 특징으로 하는 생강추출액의 제조방법을 통해서도 달성된다. In addition, the object of the present invention, the step of drying daylight or hot air to wash the selected ginger raw material water content 20 to 30%; Writing the ginger thinly to a thickness of 1 to 4 mm; At least one of homemade ginseng, jujube, schisandra chinensis, cinnamon, and bokbunja thinly written in a thickness of 1 to 4 mm; Mixing the sliced ginger, at least one of the ginseng, jujube, Schisandra chinensis, cinnamon, and bokbunja, honey in a weight ratio of 1: 0.5: 1 to 2; And it is also achieved through the method of producing a ginger extract, characterized in that the mixture comprises the step of fermenting enzyme at 35 ~ 45 ℃ for 3-4 days.
이하, 도 1에 첨부된 도면에 따라 공정별로 본 발명을 더욱 상세히 설명한다. Hereinafter, the present invention will be described in more detail according to the process according to the accompanying drawings in FIG. 1.
제 1 공정 : 생강의 건조공정First step: drying of ginger
채집된 생강 원료를 수세하고 이를 즉시 일광건조 또는 열풍건조하여 수분함량이 20~30% 정도에 이르게 한다. The collected ginger ingredients are washed with water and immediately dried in sunlight or hot air to bring about 20-30% of moisture.
이때, 생강의 수분 함량이 20% 보다 적은 경우에는 약리 성분이 잘 추출되지 않으며, 30% 보다 많은 경우에는 꿀에 의한 효소발효 공정에서 시간이 지연된다. 따라서, 생강 원료의 수분함량은 20~30%가 바람직하고, 25%가 더 바람직하다.At this time, when the moisture content of ginger is less than 20%, the pharmacological component is not extracted well, and when more than 30%, the time is delayed in the enzyme fermentation process by honey. Therefore, the water content of the ginger raw material is preferably 20 to 30%, more preferably 25%.
제 2 공정 : 생강의 절편화 공정Second Process: Ginger Sliced Process
상기 제 1 공정에 의해, 수분함량이 20~30%로 건조된 상기 생강을 두께가 1~4㎜가 되도록 얇게 썰어준다. 얇게 절편화된 생강은 꿀과의 접촉 면적이 넓어지기 때문에, 꿀에 의한 효소발효 공정에서 약리 성분의 추출이 용이해지고, 또한 그 발효 공정의 시간을 단축시킬 수 있다. By the first step, the ginger dried in a water content of 20 to 30% is thinly sliced to have a thickness of 1 to 4 mm. Since the thinly sliced ginger has a wide contact area with honey, the extraction of pharmacological components in the enzyme fermentation process with honey becomes easy, and the time for the fermentation process can be shortened.
제 3 공정 : 생강과 꿀의 혼합공정3rd process: mixing process of ginger and honey
상기 제 2 공정에 의해 절편화된 생강과 꿀을 1:1~2의 중량 비율로 혼합한다. 즉, 생강 1㎏에 꿀 1~2㎏을 첨가하는 것이 적합하다. 이때 회전날개가 구비된 혼화기나 균질기를 사용하여 1~2시간 충분히 혼화시킨다. Ginger and honey sliced by the second process are mixed at a weight ratio of 1: 1 to 2. That is, it is suitable to add 1-2 kg of honey to 1 kg of ginger. At this time, using a rotating blade equipped with a mixer or a homogenizer is sufficiently mixed for 1 to 2 hours.
만약, 생강 1㎏에 꿀을 1㎏ 보다 적게 혼합하는 경우에는, 매운맛, 쓴맛 등 생강 특유의 자극적인 맛을 충분히 제거할 수 없을 뿐만 아니라 진저롤을 원하는 양(0.4 중량%)만큼 충분히 추출할 수 없다. 또한, 생강 1㎏에 꿀을 2㎏ 보다 많게 혼합하는 경우에는, 본 발명의 효과가 포화되므로 경제적으로 바람직하지 않다. If less than 1 kg of honey is mixed with 1 kg of ginger, not only the pungent and bitter tastes such as ginger can be removed but also the ginger roll cannot be extracted as much as the desired amount (0.4% by weight). . In addition, when honey is mixed with 1 kg of ginger more than 2 kg, since the effect of this invention is saturated, it is not economically preferable.
제 4 공정 : 효소발효 공정4th process: enzyme fermentation process
제 3 공정에서 제조된 생강과 꿀의 혼합물은, 35~45℃에서 3~4일간 유지하여 효소발효시킨다. The mixture of ginger and honey prepared in the third step is maintained at 35 to 45 ° C. for 3 to 4 days to ferment the enzyme.
아울러, 본 발명은 생강과, 소정의 두께로 절편화된 인삼, 대추, 오미자, 계피, 및 복분자 중 적어도 어느 하나의 혼합물을, 상기 공정에 따라 꿀과 함께 효소발효시킬 수 있다. In addition, the present invention can enzymatically ferment a mixture of ginger and at least one of ginseng, jujube, Schisandra chinensis, cinnamon, and bokbunja sliced to a predetermined thickness with honey according to the above process.
이상, 상기에서 설명한 바와 같이, 본 발명은 매운맛, 쓴맛 등의 특유한 자극적인 맛이 상당히 감소될 뿐만 아니라 유효 성분 중 진저롤(gingerol)을 다량 함유하는 생강추출액의 제조 방법을 제공하므로, 식품산업상 매우 유용한 발명인 것이다.As described above, the present invention provides a method of preparing a ginger extract containing a large amount of gingerol in the active ingredient, as well as significantly reducing the irritating taste peculiar to spicy and bitter tastes, and thus, greatly affects the food industry. It is a useful invention.
이하, 본 발명의 구성을 하기 실시예를 들어 더욱 상세히 설명하지만 본 발명의 권리범위가 하기 실시예에만 한정되는 것은 아니다. Hereinafter, the configuration of the present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited only to the following examples.
실시예Example 1. 본 발명의 생강추출액의 제조 1. Preparation of ginger extract of the present invention
수세 정제된 생강 5㎏을 일광건조하여 수분함량 25%로 맞추었다. 상기 생강을 두께 1~4㎜가 되도록 얇게 썬 후, 그 생강 1㎏을 칭량하고, 여기에 꿀 2㎏을 칭량하여 균질기에 함께 넣고 1시간 동안 혼합하였다. 5 kg of purified ginger was dried in daylight to 25% moisture content. After the ginger was thinly sliced to a thickness of 1 to 4 mm, 1 kg of the ginger was weighed, and 2 kg of honey was weighed and put together in a homogenizer and mixed for 1 hour.
그런 다음, 상기 혼합물을 균질기로부터 인출하여 병에 담고, 마개를 막은 후에 40℃에서 3일간 유지하여 본 발명의 생강추출액을 제조하였다. Then, the mixture was taken out from the homogenizer, put in a bottle, and the stopper was kept at 40 ° C. for 3 days to prepare a ginger extract of the present invention.
상기 실시예 1에 따라 제조된 생강추출액을 도 2 및 3에서 확인할 수 있다. Ginger extract prepared according to Example 1 can be found in FIGS. 2 and 3.
또한, 생강 1㎏에, 인삼 0.5㎏ 및 꿀 2㎏을 첨가하여 40℃에서 3일간 유지하여 제조한 생강추출액을 도 4 및 5에서 확인할 수 있다. In addition, the ginger extract prepared by adding 1 kg of ginger, 0.5 kg of ginseng and 2 kg of honey and maintained at 40 ° C. for 3 days can be seen in FIGS. 4 and 5.
또한, 생강 1㎏에, 대추 0.5㎏ 및 꿀 2㎏을 첨가하여 40℃에서 3일간 유지하여 제조한 생강추출액을 도 6 및 7에서 확인할 수 있다. In addition, the ginger extract prepared by adding 1 kg of ginger, 0.5 kg of jujube and 2 kg of honey and maintained at 40 ° C. for 3 days can be seen in FIGS. 6 and 7.
실시예Example 2. 2. 대조구Control 제품의 제조 Manufacture of products
본 발명의 생강추출액의 자극적인 맛의 유무 및 진저롤 함량을 조사하기 위하여, 본 발명의 제조방법 중 단지 제 3 공정에서 꿀을 첨가하지 않을 뿐 다른 모든 제조공정은 동일하게 하여 생강추출액을 제조하였다. In order to investigate the presence or absence of the irritating taste and ginger roll content of the ginger extract of the present invention, only the third step of the manufacturing method of the present invention, the honey is not added but all other manufacturing processes were prepared in the same manner.
실시예Example 3. 관능검사 3. Sensory test
성인 10명을 무작위로 추출하여 상기 실시예 1의 생강추출액과 실시예 2의 생강추출액의 매운맛, 쓴맛 및 냄새에 대한 관능검사를 실시하였다. 맛의 경우, 매운맛 또는 쓴맛이 있는 경우 5점, 없는 경우 0점으로 평가하여 합산하였으며, 향의 경우 향이 우수한 경우 5점, 보통인 경우 3점, 나쁜 경우 0점으로 평가하여 합산하였다. Ten adults were randomly extracted and subjected to sensory tests for the pungent, bitter and odor of the ginger extract of Example 1 and the ginger extract of Example 2. In the case of taste, hot and bitter taste was evaluated by adding 5 points, and when there was no 0 points, the fragrance was added by evaluating 5 points for excellent fragrance, 3 points for normal and 0 points for bad.
그 결과를 이하의 표 1에서 확인할 수 있다. The results can be confirmed in Table 1 below.
상기 표 1에서 확인할 수 있는 바와 같이, 본 발명의 제조방법으로 제조된 생강추출액의 경우 매운맛, 쓴맛 등 생강 특유의 자극적인 맛과 냄새가 대조구 제품에 비하여 현저히 완화되었음을 확인할 수 있었다. As can be seen in Table 1, the ginger extract prepared by the production method of the present invention was found to be significantly relieved compared to the control product irritant taste and smell, such as spicy, bitter taste.
실시예Example 4. 4. 진저롤Gingerbread 함량 검사 Content inspection
상기 실시예 1의 생강추출액과 실시예 2의 생강추출액의 진저롤 함량을 평가하기 위하여 다음의 실험을 하였다. In order to evaluate the ginger roll content of the ginger extract of Example 1 and the ginger extract of Example 2 was the following experiment.
우선, 각 생강추출액을 2.0g씩 정밀하게 칭량하여 메탄올을 60㎖씩 넣고 2시간 환류 추출한 후 여과하였다. 잔류물에 메탄올을 30㎖씩 넣은 후에 다시 2시간 환류 추출한 후 여과하였다. 실시예 1의 생강추출액의 잔류물에 메탄올을 첨가하여 용량이 정확하게 100㎖가 되도록 한 시료를 SA1이라 하였고, 실시예 2의 생강추출액의 잔류물에 메탄올을 첨가하여 용량이 정확하게 100㎖가 되도록 한 시료를 SA2라고 하였다.First, each ginger extract was precisely weighed by 2.0 g, and 60 ml of methanol were added thereto, followed by extraction under reflux for 2 hours, followed by filtration. 30 ml of methanol was added to the residue, followed by further extraction under reflux for 2 hours, followed by filtration. The sample was added to the residue of the ginger extract of Example 1 so that the capacity was precisely 100ml SA 1 , and the methanol was added to the residue of the ginger extract of Example 2 so that the capacity was exactly 100ml One sample was named SA 2 .
진저롤(C17H26O4) 표준품 10㎎에 메탄올을 첨가하여 용량이 정확하게 100㎖가 되도록 한 시료를 ST라 하였다. SA1, SA2 및 ST 10μL씩을 가지고 HPLC를 측정하였다. 각 시료에 포함된 진저롤의 함량은 이하의 식에 따라 계산된다:ST was used as a sample in which 10 mg of ginger roll (C 17 H 26 O 4 ) standard was added to make the volume exactly 100 ml. SA 1 , SA 2 And HPLC were measured with each 10 μL of ST. The content of ginger roll in each sample is calculated according to the following formula:
시료의 진저롤의 함량(㎎) = 진저롤 표준품의 양(㎎)×시료의 피크면적/표준품의 피크 면적Ginger roll content (mg) = amount of ginger roll standard product (mg) x peak area of sample / peak area of standard product
(검출기 : 자외부흡광광도계(측정파장 280㎚)(Detector: ultraviolet absorption photometer (wavelength 280nm)
컬럼 : 내경 5㎛, 길이 15 ㎝ Column: inner diameter 5㎛, length 15cm
컬럼 온도 : 실온 Column temperature: room temperature
이동상 : 아세토니트릴물혼합액(45:55) Mobile phase: Acetonitrile water mixture (45:55)
유량 : 0.8 ㎖/분) Flow rate: 0.8 ml / min)
상기 표 2에서 확인할 수 있는 바와 같이, 본 발명에 따른 생강추출액(실시예 1)은 대한약전에 기재되어 있는 진저롤의 함량 기준(0.4중량%) 보다 많은 양(0.47중량%)의 진저롤을 함유하고 있었다. 반면, 꿀을 발효에 사용하지 않은 대조구(실시예 2)에 따른 생강추출액은 진저롤을 본 발명보다 훨씬 더 적게 포함하고 있음을 확인할 수 있었다. As can be seen in Table 2, the ginger extract according to the present invention (Example 1) contains the amount of ginger roll (0.47% by weight) than the content basis (0.4% by weight) of the ginger roll described in the Korean Pharmacopoeia there was. On the other hand, the ginger extract according to the control (Example 2) that does not use honey for fermentation was confirmed that the ginger roll contains much less than the present invention.
도 1은 본 발명의 제조방법을 도시한 공정도이다.1 is a process chart showing the manufacturing method of the present invention.
도 2 내지 7은 본 발명의 제조방법으로 제조된 생강추출액을 도시한 도면이다.2 to 7 is a view showing a ginger extract prepared by the production method of the present invention.
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