KR100880537B1 - Composition comprising cantaloupe melon extract and gliadin for improving ability of brain - Google Patents

Abstract

An agent for improving memory power, concentration power and learning ability is provided to enhance the mental state and the dormant state in the abnormal group. An agent for improving memory power, concentration power and learning ability comprise the combined product of an cantaloupe melon extract and gliadin as an active ingredient.

Description

Composition comprising cantaloupe melon extract and gliadin for improving ability of brain}

The present invention relates to a functional composition for improving memory, concentration, and learning ability, and more particularly, a combination of a conventional antioxidant, cantaloupe melon extract and gliadin, as an active ingredient. To improve memory, concentration, and learning ability. The combination of oral superoxide dismutase (SOD) preparation cantalop melon extract and gliadin used in the present invention improves memory, concentration, learning ability, effectively improves psychological and sleep states, It can be widely used as functional foods, food additives and pharmaceutical preparations to improve the quality.

Oxidation-reduction reactions are essential for life and resistance. The anion peroxide generated in this process causes oxidative stress such as damage to tissues, which is associated with the onset of various diseases and cell aging. Life has evolved towards strengthening antioxidant defenses to protect tissues from these oxidative stresses. Until now, studies have shown that when oxidative stress is too difficult for life, the disease occurs in the circulatory, digestive and skin tissues. It has also been reported that administration of antioxidants or anti-ischemic substances can reduce tissue damage.

Antioxidants produced in vivo include enzymes such as superoxide dismutase (SOD), catalase, glutathione peroxidase and the like. In addition, vitamin A, vitamin C, vitamin E, polyphenols, selenium, zinc, copper, and magnesium, which are consumed as supplements, help antioxidant activity. However, superoxide dismutase (SOD) and the like, as the other life, the production of the life decreases. This process leads to a weakening of the antioxidant defense mechanisms and causes various diseases and degenerative changes. Representative diseases resulting from such mechanisms include ischemic cardiovascular disease, essential hypertension, rheumatic diseases, chronic respiratory diseases, peptic ulcers and skin diseases.

However, superoxide dismutases present in nature differ in their half-life, regardless of human, vegetable, and animal enzymes, but they have little in common when consumed orally, and are rapidly destroyed by gastric acid. Have

The combination of the cantalop melon extract and gliadin is a superoxide dismutase (SOD) preparation derived from a candaloff melon extract coated with a gliadin film, wherein the gliadin is hydrophobic so that the cantalop melon It is known that the SOD activity of the extract is absorbed through the digestive system rather than disappearing during digestion. Specifically, the combination of cantaloupe melon extract and gliadin is made orally administrable by combining cantaloupe melon ( Cucumis melo LC) extract with a biopolymer of glaradine in a 40% alcohol solution. J. Ethnopharmacol. , 94: 67, 2004; J. Microencapsul ., 19: 377, 2002). In fact, wheat ( Triticum vulgare ) gliadin is hydrophobic so that the SOD activity of the melon extract is absorbed through the digestive tract without disappearing during digestion. (Internat. J. Pharmaceut. , 121: 117, 1995).

In addition, the inventors of the present invention have developed and patented a method of using a combination of oral superoxide dismutase (SOD) candaloff melon extract and gliadin as a hangover scavenger (23 May 2006) Registered, patent 10-0584891). From this, the combination of candaloff melon extract and gliadin was named as PME88 melon extract of Seafam, Korea. In addition, various studies have been conducted on the changes that occur when the combination of the cantalop melon extract and gliadin are administered in diseases caused by oxidative stress. Indeed, the combination of cantalop melon extract and gliadin in the treatment of peptic ulcer, skin disease and ischemic cardiovascular disease has been reported in terms of plasma proteins, carbohydrates, lipids and immune status, and the effects of varying dosages. .

Accordingly, the present inventors have continued to develop new uses of the combination of oral superoxide dismutase preparation cantalop melon extract and gliadin, and the combination of cantalop melon extract and gliadin is reduced to It has been found that memory, concentration, learning ability and physical condition have various diseases that are induced to improve memory, concentration, learning ability and effectively improve the physical condition such as psychological state and sleep state in the abnormal group. The present invention was successfully completed by confirming that it can be widely used as a functional food, a food additive, a pharmaceutical agent, and the like, which enhances quality.

In particular, the function of improving memory, concentration and learning ability is unpredictable from the known functions at all, and there is novelty and progress, which is a breakthrough result revealed by the continuous efforts of the present inventors.

An object of the present invention is to provide a combination of candallov melon extract and gliadin as a memory, concentration, learning improver.

In order to achieve the above object, the present invention provides a memory, concentration, learning improver containing a combination of cantalop melon extract and gliadin as an active ingredient.

Hereinafter, the present invention will be described in detail.

The present invention provides a use of a combination of cantalop melon extract and gliadin as an agent for improving memory, concentration and learning ability.

In the present invention, the combination of the cantalop melon extract and gliadin is composed of a form that can be orally administered by combining the candaloff melon extract with a biopolymer, glaadin, in an aqueous 40% alcohol solution. In the present invention, the reason why the cantalop melon extract is used in the form of a composition combined with gliadin is that the superoxide dismutase contained in the melon extract is not destroyed by gastric acid during digestion by the hydrophobic gliadin. This is because the activity does not disappear and enzymes can be easily absorbed through the digestive tract.

All products can be used as a combination of the melon extract and gliadin, but PME88 melon extract of Syspharm Korea, which is commercially available as an oral superoxide dismutase (SOD) (recognized by the Korea Food and Drug Administration) Product name).

In addition, the present invention provides a memory, concentration, learning ability improver using the combination of the cantalope melon extract and gliadin as an active ingredient.

The combination of the cantalop melon extract and gliadin used in the present invention includes canalloupe melon extract [cantaloupe melon ( Cucumis melo LC)] and gliadin, which are oral superoxide dismutase (SOD) preparations. It is preferable to use PME88 melon extract of Syspharm, Korea.

Functions of the combination of the cantalop melon extract and gliadin include concentration, learning ability (calculation, etc.), memory as well as sleep quality, psychological state, attention, association and mental thinking, and also headache, Depression. This includes the elimination of fatigue and loss of motivation and the improvement of environmental resistance, and moreover, the improvement of premenstrual syndrome in women of childbearing age.

The present invention provides a functional food that improves memory, concentration and learning ability by using a combination of cantalop melon extract and gliadin as an active ingredient.

The present invention also provides a food additive that improves memory, concentration, and learning ability by using a combination of cantalop melon extract and gliadin as an active ingredient.

In addition, the present invention can provide a pharmaceutical preparation that improves memory, concentration, learning ability by using a combination of cantalop melon extract and gliadin as an active ingredient.

In the present invention, when provided as a pharmaceutical composition for improving memory, concentration and learning ability, depending on the intended use, independently or as a pharmaceutical additive with a pharmaceutically acceptable carrier or excipient, or in any other form suitable for administration to humans Can be used. Carriers that may be contained in the pharmaceutical compositions of the present invention may include extenders, high fiber additives, encapsulating agents, lipids, and the like, examples of which are well known in the art. In addition, the pharmaceutical composition of the present invention may be administered by varying the dose depending on memory, concentration, improvement in learning ability, age, sex, physical condition, administration period, administration method, weight of the patient, meal, discharge rate, etc. .

 As described above, the present invention relates to an improvement in memory, concentration, and learning ability, which contains a combination of a conventional antioxidant agent cantalop melon extract and gliadin as an active ingredient, The combination of cantalop melon extract and gliadin, a superoxide dismutase preparation for use, improves memory, concentration, learning ability, and effectively improves psychological and sleep conditions, thus improving functional foods and foods. It can be widely used as additives, pharmaceutical preparations and the like.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited by the following examples.

Example 1 Use of a Combination of Cantalov Melon Extract and Gliadin

In the present invention, in order to investigate the effect of the combination of cantaloupe melon extract and gliadin on the improvement of memory, concentration and learning ability, oral superoxide dismutase (SOD) preparation cantaloupe melon [cantaloupe melon ( Cucumis melo LC)] extract and gliadin conjugate PME88 melon extract of Sea Palm Korea was used. The cantalope melon extract and the gliadin combination PME88 melon extract were administered 2 capsules (500mg) per day for 30 days to patients with stress-induced diseases.

Example 2 Investigation of the Effect of Cantalop Melon Extract and Gliadine on Memory, Concentration, and Learning Ability

In the present invention, in order to investigate the effect of oral superoxide dismutase (SOD) preparation cantaloupe melon ( Cucumis melo LC) extract and gliadin combination on memory, concentration, learning ability change, PME88 melon extract of Example 1 was administered to patients admitted to the hospital due to abnormal stress caused by memory, concentration, learning ability and body condition. The subjects consisted of a total of 40 patients aged 18 to 65 years, including ischemic heart disease and 20 hypertension, 10 ulcers and 10 skin diseases. At this time, the selection criteria are chronic disease patients (ischemic heart disease, essential hypertension, peptic ulcer), skin disease (psoriasis, gallbladder, other allergic diseases), mixed with the disease, lipid concentration abnormality, diabetes, biliary disorders, functional Gastrointestinal disorders were included, and active ulcers with bleeding, pyloric stenosis, digestive enzyme disorders, gluten-sensitive bowel disease, and other inadequate cases were excluded.

In addition, in order to measure changes in memory, concentration, and learning ability in the subjects, first, a questionnaire was performed before and after treatment about the symptoms, memory, concentration, learning ability, and quality of life of the patients. In addition, the physical findings and vital signs of the subjects were checked daily, blood tests and urine tests were performed on a predetermined day, and electrocardiogram and endoscopy were performed before and after treatment. In addition, divided into a randomized experimental group that is distinguished from the basic treatment group, which is the only basic medical treatment for each disease, the randomized experimental group received 2 capsules (500mg) per day for 30 days in addition to the basic treatment. On the 14th and 30th day of administration, thorough questionnaire was conducted, and on day 31, the patient was asked to answer the quality of life, and psychological tests were conducted simultaneously. Patients were also asked to keep a daily journal of their physical condition and symptoms, and vital signs were checked daily by hospital staff. Blood tests, urine tests, electrocardiograms, and endoscopy were performed 25 to 31 days after treatment. In patients with gastric ulcer, endoscopic biopsy is performed for Helicobacter and pathological examination (neutrophil, lymphocyte and plasma cell infiltration, mucosal atrophy, severity and presence of intestinal epithelial metabolism, scar tissue formation) The results were scored. Immunological parameters were measured by immunofluorescence using monoclonal antibodies against CD antigens to measure the function of lymphocytes, the concentration of immune complexes, and the concentration of antibodies. And so on. The treatment effect was determined by combining the rate at which symptoms disappeared, ECG data, endoscopic findings, and blood test findings.

As a result, 91% of patients complained of headache, depression, fatigue, loss of motivation before PME88 melon extract and 83% of patients complained of sleep disorder. At 100% after day, improvement or disappearance of these symptoms was confirmed (FIG. 1). In particular, 60% of women of childbearing age were suffering from premenstrual syndrome, which experienced improvement or loss of premenstrual syndrome after PME88 melon extract supplementation. In addition, the severity of lethargy of the patients was assessed on a multi-faceted personality test (MMPI) scale. As a result, all patients had moderate or lethargy but significantly improved lethargy after PME88 melon extract administration (see Table 1 and Table 2).

Investigate changes in psychological function variable Primary treatment When supplementing PME88 melon extract MMPI scale 115.3 ± 5.1 105.2 ± 2.2 *

* Significance level p <0.005

Investigation of Changes in Lethargy Levels with PME88 Melon Extract Indicators Basic treatment PME88 Melon SOD Supplement Memory (short-term memory, word count) word memorization (main memory, word count) 7.0 ± 0.2 5.6 ± 0.1 8.0 ± 0.3 6.8 ± 0.1 Computing power (hours, sec) 66.0 ± 0.1 44.2 ± 1.3 Attention (% of failures) 2.3 ± 0.2 0.5 ± 0.1 Social life mistake frequency (%) 4.1 ± 0.2 1.3 ± 0.3 Frequency of mistakes during exercise (%) 7.3 ± 0.3 2.3 ± 0.2

* Significance level p <0.01

In addition, the psychological test results showed that psychological state, attention, association, and intellectual ability (calculation, memorization, etc.) were improved after administration of PME88 melon extract, so that the PME88 melon extract, which is a combination of the cantalop melon extract of the present invention and gliadin, is remembered , Concentration and learning ability were found to be effective. In addition, PME88 melon extract supplementation therapy was found to significantly improve helplessness, attention, memory, computing ability, association, rapid thinking and sleep quality.

Example 3. Investigation of the improvement of memory, concentration and learning ability of the combination of cantalop melon extract and gliadin using experimental animals

In the present invention, in order to investigate the effect of the combination of the cantalop melon extract and gliadin on the experimental animals to improve memory, concentration, and learning ability, four experimental SD rats were fed with only gliadin and the remaining 12 animals. There were three different concentrations of the PME88 melon extract of Example 1 was fed. Rats were also irradiated with radiation (30 Gy / 10 times) for 12 days to induce progressive learning and memory impairment and to reduce memory, concentration and learning ability due to degenerative changes in brain cells after radiation. In addition, in order to investigate memory, concentration and learning ability change due to aging, 75 Wistar rats at 23 months of age and 15 rats at 4 months of age were divided into 6 groups of 15 rats (n = 90). In old rats, group 3 received the PME88 melon extract at doses of 0.25, 0.5, and 1.0 mg / kg, and the other two groups received gliadin alone or gave nothing as a control. To compare the response of young rats, it was used as a control without any administration. Then, the water maze test was conducted using the test animals as follows. Test medications were administered for 14 days, followed by training on the test with water-in-water mathematics (continue taking medications). The test was done by measuring the time it takes for a mouse to escape from a watery maze.

As a result, it was found that in the experimental group receiving the PME88 melon extract, the avoidance response to the bad stimulus was better, and thus the PME88 melon extract improved the behavioral disorder occurring after the radiation treatment. The time required for escape from the labyrinth of the old mice receiving the PME88 melon extract was significantly faster than that of the control group, and the result was judged to improve memory, concentration, and learning ability compared to the young mice. Therefore, the PME88 melon extract, which is a combination of the cantalop melon extract and gliadin of the present invention, improves memory, concentration and learning ability due to changes in brain cell degeneration after radiation treatment in experimental animals and also occurs during physiological aging. In addition, it was found to be helpful in suppressing concentration, concentration, and learning disabilities.

Example 4. Investigation of the effects of the combination of cantalop melon extract and gliadin on the memory, concentration and learning ability

62 healthy men and women from 15 to 34 years of age, including students who are scheduled for major exams (admission, employment, promotion, qualifications, etc.) within 3 months, company employees, and job preparation students, were divided into two groups. Rey Complex Figure Test (RCFT) was performed.

  Placebo group: Two capsules a day for 30 days taken in the same form

  Intake group: Take two capsules (500mg) for 30 days a day

* Rey Complex Figure Test (RCFT)

The Rey Complex Figure Test (RCFT) is a recognized neuropsychological test (Hamby et al ., 1993) widely used nationally and internationally for the function of the frontal lobe of the brain responsible for memory, analysis, calculation, thinking, and judgment. This test is useful for measuring memory and organizational ability, especially among tests conducted for evaluation. It consists of 36 points, and it is the most commonly used method for evaluating memory and learning ability because it has the advantage of analyzing the short-term and long-term memory and composition ability of the subject in detail by using the conversion score according to age. RCFT is a test method that is suitable for measuring memory and learning ability, and it is a test method that exactly fits the purpose of this test.

Exclusion Criteria.

1) If you have a specific disease that requires medicine or treatment

2) Anyone who has taken foods or medicines that may affect cognitive function within 30 days of participating in human testing

3) If you have eaten a dietary supplement that can affect cognitive function within 4 weeks of participating in the human body test

4) When it is impossible to evaluate test items due to difficulty in daily communication or disability

5) Inappropriate participation in other human tests

Sixty subjects participated in the study and completed the human test. The ages of the participants were 15 to 34 years old and the average age was 23.2 years old. There were 34 males and 28 females. The study group was randomized to 30 placebo and 32 intake groups. Subjects were asked to eat and smoke as usual during the test period, and alcohol was prohibited.

The RCFT test is a measure of the correlation between the constructive power and the memory power of the intention figure to see how effectively the subject can memorize and draw complex structures. Higher percentiles indicate increased memory. The placebo intake group showed no significant change (P> 0.05), while the intake group increased significantly (P <0.05) from 38.6% to 60.1% (Figure 3). Therefore, from the results of this experiment, it was found that the intake effectively improved the memory and the compositional ability of the figure, and this improvement was statistically significant.

Figure 1 shows the effect on the psychological state including fatigue, discomfort and sleep disorders when supplemented with PME88 melon extract of the present invention.

Figure 2 shows the results of the underwater labyrinth test by treating the experimental animals PME88 melon extract of the present invention.

Figure 3 is a graph showing the results of the RCFT test when the examinee ingested PME88 melon extract of the present invention.

Claims (2)

Improving memory, concentration and learning ability, which contains a combination of cantaloupe melon extract and gliadin as active ingredients delete

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