KR100865513B1 - Composition for preventing and treating obesity comprising the alcoholic extract of gang-hwa mugwort - Google Patents

Abstract

A composition for preventing and treating obesity is provided to show an effect for improving a liver function and an anti-obesity effect and not to have a side effect when users take the composition for preventing and treating obesity because the composition for preventing and treating obesity is natural food material. A composition for preventing and treating obesity has the alcoholic extract of 1 - 3 carbon number of the Artemisia princeps as an active ingredient. The gang-hwa mugwort extract is manufactured by adding the ethanol in the gang-hwa mugwort powder 2~10 times, and extracts at the room temperature for 12 ~24 hours. The extract is filtered into a centrifuge or the filter paper, precipitates are removed, and a supernatant is collected. The gang-hwa mugwort extract is manufactured into the powder form by drying the concentrated supernatant after the collected supernatant is concentrated.

Description

Composition for preventing and treating obesity comprising the alcoholic extract of gang-hwa mugwort}

1 is a graph showing the weight change of the obese mouse model induced by a high fat diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 2 is a graph showing the change in the weight of the epididymal fat of the obese mouse model induced by high fat diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 3 is a graph showing the change in lipid concentration of liver tissue of obese mouse model induced by high fat diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 4 is a graph showing the change in liver weight of the obese mouse model induced by a high fat diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 5 is a graph showing the change in blood leptin concentration of the obese mouse model induced by a high fat diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 6 is a graph showing the weight change of the mouse model of arteriosclerosis disease induced by high fat-high cholesterol diet according to the administration of the extract of strengthening wormwood of the present invention.

Figure 7 is a graph showing the changes in liver function indicators of the mouse model of arteriosclerosis disease induced by high fat-high cholesterol diet according to the administration of the extract of strengthening wormwood of the present invention.

The present invention relates to fortified wormwood extract for the prevention and treatment of obesity.

As the national economy develops, obesity has become a threat to national health as people consume more processed foods with high energy content and have less exercise. Obesity is awkward and unnatural appearance, not only gives a bad image to the viewer and discomfort in activities, but also obese people compared to normal people, diabetes, arteriosclerosis, hyperlipidemia, heart disease, liver disease, gallstones, gout, kidney disease It is reported that the risk of surgery and surgery is largely short-lived. Therefore, obesity is a life issue for people over the middle age, and young women are considered seriously, especially for cosmetic reasons.

Currently, appetite suppressants, heat generators, and diuretics are used as obesity treatment agents. Reductil ^TM (Abbott, USA), the most commonly used obesity treatment agent, is an appetite suppressant and forms a market of more than US $ 100 million a year. Doing. However, edible inhibitors for the treatment of obesity have side effects such as increased blood pressure, dependent diarrhea, constipation, insomnia, and anxiety. Therefore, there is a great interest in the use of various food materials whose safety and physiological functions have been proven for many years to prevent obesity. It is rising. Currently, products containing dietary fiber, herbal medicines and various food ingredients have been developed as a health food for obese people. It is known to be obese. Therefore, there is an urgent need to reduce such side effects and to develop a prophylactic and therapeutic agent with excellent safety even in long-term use.

On the other hand, Artemisia is a perennial herbaceous plant of the compositae, which is distributed in Korea, Asia and Europe. Ganghwa wormwood is a special product that grows only in Ganghwa area and is used for medicinal purposes. In other words, fortified wormwood contains eupatillin (anticancer component), postural osdine (anti-ulcer component), apigenin, aipogenin, eupafolin, amylase, choline, aldehydes, eucalyptol, essential oils, vitamins, Proteins, calcium, magnesium, iron, potassium and phosphorus are contained, the efficacy of which is known as anti-inflammatory, analgesic, cardiac, antitussive action. However, there has been no report on the effect of preventing or treating obesity.

Accordingly, the present inventors have tried to develop a food material that can effectively reduce body fat without ingesting side effects even if it is ingested as a natural food material that can effectively prevent and suppress obesity, without affecting normal social life. While repeating the physiological efficacy experiments of the material extracts confirmed that the strengthening wormwood extract has an excellent effect on the prevention and inhibition of obesity was completed the present invention.

Therefore, it is an object of the present invention to provide a composition for the prevention or treatment of obesity, containing the extract of Fortified Mugwort as an active ingredient.

In order to achieve the object of the present invention as described above, there is provided a composition having a preventive or therapeutic effect of obesity containing an alcohol extract of 1 to 3 carbon atoms of fortified wormwood as an active ingredient, and a method of manufacturing the same.

Hereinafter, the present invention will be described in more detail.

In Ganghwa, the air is not dried up due to oceanic climate, and the temperature is about 1 ℃ lower in summer than in other regions, and there is no great cold in winter, and the quality of mugwort is good because the sea breeze, seaweed, and sunshine are mixed with base. Fortified wormwood is preferably the lion footwort and wormwood growing wild in Ganghwa. Lion Mugwort ( Artemisia The ingredients of princeps Pampanini cultivated Sajabal) include eupatillin, zodiacin, daukosterol, sesamin, apogenin, eufapoline, artemisine, carotene, cineol, adenine, choline, tetradecatrin, etc. Actions, bronchial dilatation, capillary action, anticoagulant action, anti gastritis and anti gastric ulcer effect are known. Also, Zucchini ( Artemisia The ingredients of princeps Pampanini cultivated Sajuari) include eupatillin, posture oscillin, hispiduline, priedelin, stigmasterol, beta amiline, dowosterol, ergosperol peroxide, and phytol. Immunostimulating, anti-gastritis and anti-gastric ulcer effects are known. However, it has never been reported that fortified wormwood is associated with obesity inhibition and liver function improvement.

Fortified wormwood extract of the present invention can be prepared using a known method. For example, the extract is a crude extract or a non-polar solvent soluble extract, wherein the crude extract is extracted with a solvent selected from water, lower alcohols (for example, ethanol, methanol, propanol) or a mixed solvent thereof, non-polar solvent Soluble extract may be extracted by dipping or heating at room temperature by adding a nonpolar solvent such as hexane, ethyl acetate, ether. Preferably, fortified wormwood extract of the present invention can be prepared by immersion extraction at room temperature using an alcohol having 1 to 3 carbon atoms.

The ratio of the strengthened wormwood and the extraction solvent at the time of solvent extraction is not particularly limited. It can be used 2 to 10 times (by weight).

The temperature at the time of extraction is preferably carried out at room temperature under atmospheric pressure, the extraction time depends on the extraction temperature, but from 12 hours to 48 hours, preferably For 12 hours ~ 24 hours Can be extracted. In addition, the extraction efficiency can be further increased when stirring with a shaker (shaker) during the extraction.

Fortified wormwood used for extraction can be washed and harvested or used as is or dried. Drying can be dry, shade, hot air and natural drying. In addition, in order to increase the extraction efficiency may be used to grind the reinforcement wormwood or its dry matter with a grinder.

Preferably, fortified wormwood extract of the present invention is added to ethanol 2 to 10 times, preferably 5 to 10 times to the strengthened wormwood powder prepared by pulverizing dried medicinal herbs and extracted for 12 hours to 24 hours at room temperature. The extract is filtered by centrifugation or filter paper to remove the precipitate and recover the supernatant. The recovered supernatant can be concentrated and dried to powder.

Fortified wormwood extract of the present invention is characterized in that it has the effect of preventing or treating obesity. In addition, the obesity-related metabolic diseases include type 2 diabetes, fatty liver, hyperlipidemia, cardiovascular disease, arteriosclerosis.

The present invention confirms the effect of preventing or treating obesity extract of fortified wormwood extract through in vivo experiments using LDL receptor-targeted mouse model administered with high fat diet and ICR mouse induced high fat-high cholesterol diet. It became.

Fortified wormwood extract of the present invention effectively inhibited weight gain in obesity-induced disease mouse and LDL receptor mouse model, inhibited epididymal fat accumulation, decreased lipid content of liver tissue, abnormally increased plasma leptin content, abnormally It was found that there is normalizing activity of the increased plasma liver function index.

Therefore, it provides a composition for the prevention or treatment of obesity, containing the enhanced wormwood extract of the present invention as an active ingredient.

In addition, fortified wormwood extract according to the present invention can be prepared in the form of food for the purpose of preventing or improving obesity. Therefore, the present invention provides a food composition containing the strengthened wormwood extract as an active ingredient. The food composition of the present invention includes all forms such as functional foods, nutritional supplements, health foods and food additives. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, the fortified wormwood extract itself of the present invention may be prepared in the form of tea, juice and drink for drinking, or granulated, encapsulated and powdered. Functional foods also include beverages (including alcoholic beverages), fruits and processed foods (e.g. canned fruit, canned foods, jams, marmalade, etc.), fish, meat and processed foods (e.g. ham, sausage corned beef, etc.), Breads and noodles (e.g. udon, soba, ramen, spaghetti, macaroni, etc.), fruit juices, various drinks, cookies, malts, dairy products (e.g. butter, cheese, etc.), edible vegetable oils, margarine, vegetable protein, retort food, Frozen food, various seasonings (eg, miso, soy sauce, sauce, etc.) can be prepared by adding the fortified wormwood extract of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples.

However, the scope of the present invention is not limited by these Examples and Experimental Examples.

< Example  1>

Fortified Wormwood  Preparation of Extract

<1-1> Lion  Ethanol extract manufacturer

One kilogram of lion cucurbita (2 year old, obtained from Ganghwa-gun Agricultural Technology Center Specialty Crop Research Institute) was ground using a dry mill. 1 L of 95% ethanol was added to 100 g of the ground product, followed by extraction for 12 hours with stirring. The extraction process was repeated twice. The extract obtained above was filtered through a filter paper (Advantec N0.2, filter paper) to remove the precipitate to obtain a supernatant. The supernatant was concentrated with a vacuum concentrator. The yield of the extract (percentage of extract obtained relative to the weight of lion cubs used) was 5.0%.

<1-2> Wormwood  Ethanol extract manufacturer

One kilogram of sauerkraut (2 year old, obtained from Ganghwa-gun Agricultural Technology Center Specialty Crop Research Institute) was ground using a dry mill. 1 L of 95% ethanol was added to 100 g of the ground product, and extracted, filtered, and concentrated in the same manner as in Example <1-2>. The yield of sauerkraut extract was 3.8%.

< Experimental Example  1>

Orally administered Fortified Wormwood  Extract of Obesity-induced Mice In obesity  Investigate impact

<1-1> Experimental Animal Design and Experimental Composition

Experimental animals were used to purchase male ICR mice (6 weeks old) through the central laboratory animals. The pretreated animals were adapted to the laboratory environment with free feeding of solid feed and water for 2 weeks, and then 8 weeks old (32.3 ± 0.3 g) mice in good health were used for the experiment. Normal control group (n = 6), high fat diet containing 25% beef tallow, Dyets.co. Ltd, divided into 5 groups, and eating a normal diet (AIN-76A rodent purified diet, Dyets. Co. Ltd.) Obese control group (n = 8) and control diets were supplemented with two groups (2 mg / kg / day) supplemented with lion wormwood ethanol extract (50 mg / kg / day) and sauerkraut ethanol extract (50 mg / kg / day). N = 8) and the anti-obesity drug Xenical (10 mg / kg / day), which is a fat absorption inhibitor, were divided into drug control groups (n = 8 each), and they were given an 8-week experimental diet. The ethanol extract of lion cucurbita and sauerkraut ethanol extract was dissolved orally in 0.2 ml of sterile distilled water containing 0.3% tween 80 such that 50 mg / kg / day and Xenical supplement were 10 mg / kg / day. The environment of animal breeding room was maintained at constant temperature (25 ± 2 ℃), humidity (50 ± 5%), and photoperiod of 12 hours (light on 07: 00 ~ 19: 00), and animals were 3 ~ 4 The animals were kept separately and fed freely with diet and drinking water (ad libitum). Dietary intake and body weight were measured and recorded at a fixed time every week, with blood collected from the posterior orbital vein using a capillary tube, and then coagulated with EDTA to prevent coagulation. Plasma was separated by centrifugation at 4 ° C. for 15 minutes and stored at −70 ° C. until analysis. After the animal was bred for 3 hours before sacrifice, blood was collected and treated in the same way.The organ tissues (liver and adipose tissue) of each animal were extracted immediately after blood collection and quenched with liquid nitrogen immediately after weighing. And stored at -70 ° C.

<1-2> Changes in Weight and Organ Weight of Experimental Animals

Weight gain before and after 8 weeks of high-fat diet increased 6.8 g from 32.4 ± 1.4 g to 39.2 ± 1.5 g in normal control (RD), while 43.5 ± 3.7 g in obese control (HFD) from 32.3 ± 1.3 g Increased by 11.2 g. At this time, lion wormwood ethanol extract administration group (SJ) increased 7.5 g from 32.3 ± 0.8 g to 39.8 ± 2.1 g, Sasang wormwood ethanol extract administration group (SS) increased 8.8 g from 32.3 ± 1.1 g to 41.2 ± 3.9 g, Xenical dose control group (XEN) increased by 7.2 g from 31.7 ± 1.2 g to 38.9 ± 3.0 g (FIG. 1). Inhibition of weight gain on HFD was 33% in SJ, 21% in SS, and 36% in XEN.

1 is a graph showing the weight change of the obese mouse model induced by a high fat diet according to the administration of the extract of strengthening wormwood of the present invention. The figures represent mean ± standard error (n = 8). * The letter means that there is a significant difference between each group at the p <0.05 compared to the obese control group.

<1-3> Inhibitory effect of epididymal fat accumulation

Epididymal fat accumulation in normal diet and high-fat diets during the eight-week trial period was approximately RD 2.63 g / body weight 100 g and HFD 4.03 g / body weight 100 g. It was found to increase by a factor of two. Under the same conditions, SJ was 3.60 g / body weight 100 g, SS was 3.56 g / body weight 100 g, and XEN was 2.91 g / body weight 100 g.

Figure 2 is a graph showing the change in the weight of the epididymal fat of the obese mouse model induced by high fat diet according to the administration of the extract of strengthening wormwood of the present invention. The figures represent mean ± standard error (n = 8). The # character means that there is a significant difference in the obesity control group compared to the normal group at p <0.05.

<1-4> Lipid content and liver function improvement effect of liver tissue

Lipid composition of liver tissue was extracted according to the method of Folch et al. (1957), and then a certain amount of tissue was grind, 5 ml of chloroform: methanol (2: 1) solution was added and shaken at 37 ° C overnight. The extract was filtered through Whatman No. 1 filter paper, dried with nitrogen gas at 60 ° C, dissolved in 1 ml of 1% Triton X-100 based chloroform, and dried again with nitrogen gas. After 2 ml of distilled water was added and incubated at 37 ° C. for 15 minutes, total cholesterol and triglycerides were quantified by enzyme reaction method using Asan Pharmaceutical kit.

At the induction of obesity by an 8-week high fat diet, the total fat weight of the liver was significantly increased by 75% in HFD (103.9 ± 13.4 mg / g liver) compared to RD (59.3 ± 14.3 mg / g liver) (p <0.05 ), SJ, SS and XEN decreased by 12%, 7% and 21% compared to HFD. Hepatic triglyceride concentration (4.1 ± 1.1 mg / g liver) of HFD increased 41% compared to RD (2.9 ± 0.2 mg / g liver) and SS inhibited accumulation of 4.9% hepatic triglyceride concentration compared to HFD.

Figure 3 is a graph showing the change in lipid concentration of liver tissue of obese mouse model induced by high fat diet according to the administration of the extract of strengthening wormwood of the present invention. The figures represent mean ± standard error (n = 8). The # character means that there is a significant difference in the obesity control group compared to the normal group at p <0.05. * The letter means that there is a significant difference between each group at the p <0.05 compared to the obese control group.

Hepatic triglyceride concentrations of HFD fed 8-week high-fat diets were 41% higher than those of normal diets, resulting in a slight increase and hepatic liver fat. SJ (4.51 ± 0.43 g / 100 g body weight), SS (4.79 ± 0.71 g / 100 g body weight), XEN (4.55 ± 0.74 g / 100 g body weight) Suppressed liver enlargement due to high fat diet.

Figure 4 is a graph showing the change in liver weight of high fat diet-induced obese mouse model according to the administration of the strengthening wormwood extract of the present invention. The figures represent mean ± standard error (n = 8).

<1-5> Effect of Plasma Leptin Concentration

Blood levels of leptin secreted from adipocytes were measured by kit (R & D systems, Minneapolis, USA) using the Enzymed Linked Immunosolvent Assay (ELISA) method. Mouse leptin-specific antibodies were placed in each well of the Maxicorp immunoplate at a concentration of 2.5 μl / ml and fixed at 37 ° C. for one hour. In order to prevent nonspecific binding, phosphate rounds containing 3% (w / w) bovine serum albumin and 0.05% Tween-20 were reacted at 37 ° C. for 1 hour, followed by 100 μl of mouse plasma. React again. 100 μl of leptin-specific antibody labeled with biotin was added at a concentration of 250 ng / ml and reacted under the same conditions, followed by dilution with 200: 1 of streptavidin binding to biotin. After each reaction, the plate was washed three times with phosphate buffer containing 0.05% Tween-20, and after the streptavidin reaction, three more washes were performed with phosphate buffer to prevent nonspecific reactions. 100 μl of 3,3 ', 5,5'-tetramethylbenzidine, a color developing reagent was added, and 50 μl of 2 MH ₂ SO ₄ was stopped. The absorbance of 450 nm was measured using an ELISA reder.

Leptin is known as an edible regulator of leptin, which is produced by the obese gene of fat cells in proportion to body fat mass and stimulates the full stomach in the hypothalamus of the brain. Compared with RD (14.0 ± 13.0 ng / ml plasma), leptin concentration of HFD (26.6 ± 20.0 ng / ml plasma) was significantly higher due to high fat diet, SJ (13.2 ± 12.6 ng / ml plasma), XEN (17.8 ± 15.9) ng / ml plasma) showed significantly lower leptin concentrations than HFD. In contrast, SS (27.1 ± 13.0 ng / ml plasma) showed little difference from HFD. These leptin concentrations showed a tendency to be consistent with the inhibitory effect on weight gain.

Figure 5 is a graph showing the change in blood leptin concentration of the obese mouse model induced by a high fat diet according to the administration of the extract of strengthening wormwood of the present invention. The figures represent mean ± standard error (n = 8).

< Experimental Example  2>

Investigation of the effects of fortified wormwood extracts on dietary and anti-obesity in arteriosclerosis mice

<2-1> Experimental Animal Design and Experimental Composition

Male C57BL / 6J mice (8 weeks old), which were experimental animals, were purchased from Central Experimental Animal Co., Ltd., and male LDLr k / o mice (8 weeks old) were imported from Jackson Lab. The pretreated animals were adapted to the laboratory environment with free feeding of solid feed and water for 2 weeks, and then 10 weeks old mice in good health were used for the experiment. Normal control group (n = 10), high-fat-high cholesterol diet (21% Fat, 0.15% cholesterol,) to eat normal diet (AIN-76A rodent purified diet, Dyets. Co. Ltd.) Dyets.co. Ltd.) and the arterial sclerosis control group (n = 10) and high fat-high cholesterol diets of lion cucurbita ethanol extract (1% wt / wt diet) or saponica ethanol extract (1% wt / wt The diet was divided into two test groups (n = 10 each) and they were given an 8-week diet. The environment of the animal feeding room was maintained at constant temperature (25 ± 2 ℃), humidity (50 ± 5%), and photoperiod of 12 hours (light on 07: 00 ~: 00). Breeding separately, diet and drinking water was freely intake (ad libitum). Dietary intake and body weight were measured and recorded at a fixed time each week. Blood samples were collected from the posterior orbital vein using capillary tube at 0, 1, 2, 4, 6, and 8 weeks, and coagulation was prevented using EDTA. Plasma was isolated by centrifugation at 3,000 rpm (4 ° C.) for 15 minutes within 30 minutes after blood collection and stored at −70 ° C. until analysis. After the animal was bred for 3 hours before sacrifice, blood was collected and treated in the same way.The organ tissues (liver and adipose tissue) of each animal were extracted immediately after blood collection and quenched with liquid nitrogen immediately after weighing. And stored at -70 ° C.

<2-2> Changes in Body Weight and Organ Weights of Experimental Animals

Body weight gain before and after 8 weeks of high fat-high cholesterol diet increased 5.1 g from 23.0 ± 1.2 g to 28.1 ± 2.6 g in normal control group, whereas atherosclerosis control group increased from 22.2 ± 1.5 g to 31.7 ± 3.2 g. 9.5 g increased. At this time, the lion wormwood ethanol extract administration group increased 5.7 g from 22.3 ± 1.8 g to 28.0 ± 2.2 g, and the sarcasmium ethanol extract administration group increased 6.2 g from 22.6 ± 0.7 g to 28.8 ± 3.4 g. Inhibition of weight gain in the atherosclerosis control group inhibited weight gain by 40% in S. ethanol extract treatment group and 35% in S. ethanol extract administration group, similar to normal control group.

Figure 6 is a graph showing the weight change of the mouse model of arteriosclerosis disease induced by high fat-high cholesterol diet according to the administration of the extract of strengthening wormwood of the present invention. Numbers represent mean ± standard error (n = 10). The # character means that there is a significant difference in the atherosclerotic disease control group compared to the normal group at p <0.05. * The letter means that there is a significant difference in each group at p <0.05 compared to the control group of atherosclerosis.

<2-3> Improvement of Plasma and Liver Function Indicators

After obtaining the separated plasma of the normal control group and the control group of atherosclerosis disease, which were fed normal diet and high-fat high-cholesterol diet for 8 weeks, GOT and GPT were used as biomarkers of Hitachi-720 (Hitachi edical). , Japan).

Plasma GOT levels were increased by 25% in the atherosclerotic disease control group (146.0 ± 68.0 mg / dl) compared to the normal control group (116.8 ± 38.8 mg / dl). ± 20.4 mg / dl) decreased by 29% and 23%, respectively, compared to the atherosclerotic disease control group, which was lower than the normal control group. Plasma GPT levels were lower than those of the normal control group (24.0 ± 7.3 mg / dl). 54.9 ± 31.4 mg / dl), 2.3-fold increase, while lion cubs (28.2 ± 6.8 mg / dl) and sacrum mugwort (39.4 ± 12.7 mg / dl) decreased by 49% and 28%, respectively, compared with the control group of atherosclerosis. Almost the same level as normal control. In other words, it was found that the administration of the lion cucurbita extract and the extract of sauerkraut extract has an anti-obesity effect without burdening the liver at all.

Figure 7 is a graph showing the changes in liver function indicators of the mouse model of arteriosclerosis disease induced by high fat-high cholesterol diet according to the administration of the extract of strengthening wormwood of the present invention. Numbers represent mean ± standard error (n = 10). The # character means that there is a significant difference in the atherosclerotic disease control group compared to the normal group at p <0.05. * The letter means that there is a significant difference in each group at p <0.05 compared to the control group of atherosclerosis.

Fortified wormwood extract of the present invention can be useful in the prevention and treatment of obesity because it shows the anti-obesity effect and liver function improvement effect as shown in the results of the experimental examples.

Claims (2)

A composition having the effect of preventing or treating obesity, containing an alcohol extract of 1 to 3 carbon atoms of Artemisia princeps Pampanini cultivated Sajabal as an active ingredient. delete

Images