KR100859531B1 - Methods for manipulating air cells within avian eggs - Google Patents

Abstract

When the eggs are rearranged, the eggs are accelerated to separate the inner and outer corneas, allowing the movement of the air cells in the eggs. By separating the inner and outer corneas of the eggs, the air cells will be repositioned themselves in the upper portion of the egg shell. Eggs may be accelerated in a variety of ways in accordance with embodiments of the present invention, including but not limited to shaking, "frying" by hand, spinning, rotating, centrifugation, and the like.

Description

Method for manipulating air cells within avian eggs}

RELATED APPLICATION This application claims benefits under U.S. Provisional Application No. 60 / 501,644, filed September 9, 2003, the entire contents of which are incorporated by reference as if fully set forth herein.

The present invention relates to eggs, and more particularly to a method of treating eggs.

In poultry hatchery and egg processing facilities, eggs are handled and processed in large quantities. The term "treatment" refers to the administration of drugs, nutrients, hormones, and / or other beneficial substances to living eggs when the embryo is in the egg (ie, in ovo). It includes.

Intracolonal injection of various substances into the eggs has been used to reduce mortality and mortality after hatching, to increase the resulting bird's ultimate size or potential growth rate, and to influence the sex determination of embryos. .

Vaccination of live eggs has been used effectively for the immunity of birds in eggs. There are many uses for injection into eggs containing early embryos of birds. For example, it may be desirable to deliver material to early embryos, such as blastocysts. For example, manipulating early embryos in eggs for introduction of extracellular cells into the developing embryo (ie, the birth of a processed bird) or introduction of foreign nucleic acid molecules (ie, the birth of a transgenic bird) is likely to be possible in poultry. It may be desirable in the industry. Unfortunately, blastoderm visibility is very poor due to the presence of the inner shell membrane of the eggs. Moreover, the placement of the blastocyst may not be the best location for receiving the material.

In addition, it is often desirable to remove a sample from eggs, including early embryos of birds. Furthermore, it is often desirable to insert a detection device inside an egg containing an embryo and collect information therefrom.

Current methods for inserting devices into avian eggs containing early embryos result in unacceptably low hatching rates, introducing air bubbles into the eggs, and extra-embryonic membranes. Or other problems, such as damage to the embryo itself, or a combination thereof. Therefore, there is a need in the art to improve the method of inserting a device into a new egg.

In light of the above discussion, a method of manipulating an air cell in a grain is provided. Applicant accidentally discovered that the air cells in the eggs could be moved. Previously, it was believed that the egg's inner and outer corneas could not be separated from each other by fusion in parts other than air cells. Applicant accidentally discovered that even if the inner and outer corneas had linkages and connections, they could be separated and allowed for movement of the air cell. Applicants have also found that hatching rates of nursery eggs are not reduced after the air cell has been moved in accordance with embodiments of the present invention and subsequently according to commercial care practices.

According to an embodiment of the present invention, the eggs are accelerated to separate the inner and outer corneas from the eggs so that the air cells in the eggs are allowed to move when the eggs are rearranged. By separating the egg's outer and inner corneas, the aircell will reposition itself at the top of the egg shell when the eggs are placed to their sides. Eggs may be accelerated in a variety of ways in accordance with embodiments of the present invention, including but not limited to shaking, "frying" by hand, spinning, rotating, centrifugation, and the like.

The movement of the air cell according to an embodiment of the present invention may be desirable because it facilitates access through the egg shell of the egg side. The egg's flank is where the blastocyst (or blastocyst, hereinafter) can be placed more predictably, and the egg white between the egg yolk and the egg shell is relatively less at the egg's flank (as opposed to the blunt end). Visibility of the blastocyst through the cornea is greatly improved.

According to an embodiment of the present invention, a method of injecting a substance into a bird including a blastocyst and an air cell accelerates the egg so that the inner cornea and the outer cornea are separated to allow movement of the air cell in the egg when the egg is rearranged. Steps. The eggs are then oriented in a predetermined arrangement (eg, entirely horizontal or laterally), which causes the air cell to be moved to a position below the portion facing the top of the egg shell. The eggs are stored in a lateral arrangement throughout for a predetermined period of time to allow the blastocyst to be placed on its own side and beneath or adjacent to the air cell. By one of many methods known to those of ordinary skill in the art, a small opening may be introduced in the portion facing the top of the egg shell. For example, the opening of the shell may be made by drilling the shell, removing the shell, and then removing a portion of the outer cornea. Alternatively, the opening of the shell may be made by drilling or punching the shell and cornea so that both the shell portion and the outer corneal portion are removed at the same time.

The device is then introduced into the egg through a small opening in the egg shell. The device is inserted through the air cell and penetrates the inner cornea. One or more substances are placed through the delivery device and placed in or near the ventral lobe. One or more substances may be put in other positions in the egg. Thus, injection through the preferred air cell is facilitated regardless of the placement of the eggs.

According to embodiments of the invention, it may be desirable to endanger cells in the recipient embryo prior to injecting the substance (eg donor cells) into the egg. Risks to cells can be carried out by a variety of methods, including heating, cooling, coring, radiation exposure (eg, X-rays, ultraviolet radiation, infrared rays, gamma rays, radio frequency), ammonia and / or other solvents. Exposure to, etc., but is not limited thereto.

According to an embodiment of the invention, material may be extracted into a sample from one or more locations in the egg. For example, one or more samples (eg, blastocyst cells) can be removed from or near the blastocyst. One or more samples may be extracted from the outer part of the embryo (eg egg yolk or egg white). For example, samples may be extracted from egg whites to determine whether they are contaminated with microorganisms (eg Salmonella) inside egg whites. A sample can be removed from the egg to obtain information from the egg. The sample can be removed, for example, in connection with the method of discriminating the viability and sex of the embryo. The sample can be removed, for example, to perform genetic experiments with traits, pathogens and the like.

According to an embodiment of the present invention, a detector may be inserted into the egg to obtain various types of information from the inside of the egg. The detector may be inserted into a position outside the embryo (eg, egg yolk or air cell).

  Or the detector can be placed near the embryo as described above. The detector may be used to collect information such as embryo size, embryo location, embryonic development stage, and / or any characteristics of the embryo, embryo sex, and / or embryo viability, but the information is limited thereto. It doesn't happen.

The detection device can obtain information about the descent and the position of the embryo. Various detectors may be used to detect physical or chemical parameters including, but not limited to, electrical sensors, optical sensors, chemical sensors, temperature sensors, acoustic sensors, pressure sensors, or other sensors.

According to an embodiment of the present invention, after material injection and / or sample removal and / or information detection have been performed on the egg, the device is taken out of the egg. The small openings of the egg shells can be sealed by a sealing material and the eggs can be incubated until undergoing hatching or other treatment.

According to another embodiment of the present invention, the inner egg shell and outer cornea of the egg can be physically separated without accelerating the egg. For example, a hole is formed in the egg shell, and a blunt probe or other object can be inserted therein and used to separate the inner and outer cornea from the edge of the egg shell. .

1 is a side cross-sectional view of a conventional egg.

2A and 2B show that there is no movement of the air cell therein even after the conventional eggs are rearranged.

3 is a side cross-sectional view of a bird showing a state in which the egg is accelerated to separate the outer and inner cornea according to an embodiment of the present invention.

4 is a flow chart illustrating an operation of inserting into an egg according to an embodiment of the invention.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings, in which preferred embodiments of the present invention are shown. However, this invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; Rather, these embodiments are provided so that this disclosure will be thorough, and will fully convey the scope of the invention to those skilled in the art.

In the drawings, the thickness and components of the lines may be enlarged for clarity. When a component is described as being on another component “on,” it should be understood that this may mean that there may be other components directly on or between other components. In contrast, where a component is described as being "on" another component "directly," there is no other component intervening in between. When a component is described as being "connected" or "attached" to another component, it should be understood that this may be directly connected or attached to another component, or there may be other components interposed therebetween. something to do. In contrast, when a component is described as being "directly connected" or "directly attached" to another component, there is no component intervening therebetween. The terms "upward", "downward", "vertical", "horizontal", and the like are used for illustrative purposes only.

Unless defined otherwise, all scientific and technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are used for the purpose of describing particular embodiments and are not intended to limit the scope of the present invention. As used in the description of the invention and the appended claims, the singular forms “a”, “an” and “the” include the same meaning as used in the plural unless the context clearly dictates otherwise. All publications, patent applications, registered patents, and other references mentioned herein are incorporated by reference in their entirety.

The term "and / or" as used herein is meant to include any and all combinations of one or more of the matters listed therein.

The term "bird" as used herein includes females and males of any bird, but is intended to include poultry commercially raised for eggs or meat or as a pet. The term "bird" thus encompasses a variety of birds, including chickens, turkeys, ducks, geese, quails, pheasants, macaws, parrots, cockatoo, cockatiel, ostrich, emu, etc. It is intended to, but is not limited to such.

As used herein, the term "early embryo" refers to the embryo of a bird from the time of spawning (germ leaf stage) to the developmental stage at which primordial germ cells (PGCs) migrate. . Especially with regard to embryos of chickens, "early embryos" are generally before embryos of approximately embryonic 20 (H & H). The developmental stages of chicken embryos are well known in the art, for example, 'B.'s developmental diagram of R. Bellairs & M. Osmond, published by the Academic Press in 1998. See The Atlas of Chick Development.

The term "germ leaf" as used herein has a meaning understood in the art. In general, blastocysts include embryos from the time of laying to the end of intestinal embryogenesis. Such blastocysts are often referred to differently in the art, such as "germinal discs" or "embryonic discs." Blastocysts can be described as flat discs of cells that form in the eggs of early embryos and persist to the end of gastroulation. At the time of spawning, two main areas of the blastocyst are visible, the central part of which is pellucida and the surrounding part of opaca (the development of chicks by Al Velares and M. Osmond, published by Academic Press, 1998) Illustration "). Particularly with regard to embryos of chickens, the blastocysts can typically be characterized as embryos from the time of laying (ie, stage IX or stage EG & K) to approximately XIII stages (EG & K) or more.

As used herein, the term "injection" encompasses a method of inserting a device into an egg or embryo, which is a method of delivering or releasing a substance into an egg or embryo, removing a substance (ie, a sample) from the egg or embryo. Methods, and / or inserting a detection device into an egg or embryo.

The term "processed bird" or "processed embryo" refers to a recipient bird or embryo having cells from other birds or embryos, respectively called "donors". The terms “transgenic birds” and transgenic embryos are used herein with the meanings commonly understood in the art: Transgenic birds or transgenic embryos comprise nucleic acid sequences external to one or more cells.

The term "membrane" as used herein refers to any tissue layer inside the egg. Examples of the membrane inside the egg include an outer shell membrane, an inner shell membrane, a chorio-allantoic membrane, a VM membrane, and an amniotic membrane, but are not limited thereto. It is not.

Referring now to FIG. 1, a new egg 10 is shown. The illustrated egg 10 is at the shell 12, the outer egg shell 14, the inner egg shell 16, and the blunt end of the egg 10 and is located between the outer egg shell 14 and the inner egg shell 16. An air cell 18. In addition, the egg 10 shown includes egg yolk 20 and blastocyst 22, which are inner thin albumen 24a, outer thick albumen 24b, and outer yolk bag (24). surrounded by outer thin albumen (24c). As shown in FIGS. 2A-2B, the air cell 18 remains substantially stationary at the blunt end of the egg 10 regardless of the placement of the egg 10. This is because the outer egg shell 14 and inner egg shell 16 surround the air cell 18 and keep the air cell 18 in its fixed position.

According to embodiments of the present invention, an egg is accelerated to separate the outer and inner cornea of the egg from the inside of the egg so that the air cells in the egg can move when the egg is rearranged. As shown in FIG. 3, the outer egg shell membrane 14 and inner egg shell membrane 16 are separated, and the air cell 18 is adjacent to an upper portion of the egg shell 12 of the eggs 10 arranged horizontally. Its position is free. By separating the outer cornea 14 and inner cornea 16, the air cell 18 will reposition itself at the top of the egg shell 12. The air cell 18 moves along the egg in an acceleration direction (ie the outer and inner egg shells are separated from each other in an area along the sides of the egg), but the inner egg shell 16 is attached to all other parts. Maintain state. Therefore, after the acceleration of the egg, the air cell 18 will only allow the egg to be oriented (orienting) so that the area where the outer egg shell membrane 14 and the inner egg shell membrane 16 are separated is at the top of the egg. It will rearrange itself on the upper side of the interior.

Eggs may be accelerated in a variety of ways in accordance with embodiments of the present invention, which may include agitation, "flicking" by hand, spinning, rotating, centrifuging, and the like. Including but not limited to. According to an embodiment of the present invention, in order to separate the outer cornea 14 and inner cornea 16 of the eggs without causing damage to any membrane, approximately 50 m / s ² And an acceleration between approximately 500 m / s ² is used. When centrifuged, an acceleration of at least approximately 5 times (5 g) of gravity acceleration is used.

4 is a method of injecting a bird according to an embodiment of the present invention. It should be noted that the functions described in the blocks may not be performed in the order shown in FIG. 4. Two (or more) blocks described in succession may in fact be executed substantially concurrently or at other times in reverse order depending on the functionality involved. For example, the step of compromising the cells in the egg may be performed before or after the step of introducing an opening into the egg.

The eggs, including the blastocyst and the air cells, are accelerated to separate the outer and inner corneas of the eggs so that the air cells inside the eggs move when the eggs are rearranged (block 100). As described above, the air cell has conventionally been in a substantially fixed position between the outer and inner corneas of the egg. Various methods of accelerating eggs are described below.

In some embodiments, it may be desirable to “compromise” cells in recipient embryos prior to introduction of donor cells (block 110). As used herein, the term "risk" is used on embryonic cells so that exogenous cells can proliferate and gain dominance due to the slowing or termination of endogenous cells. This means that some abuse is done. The risk to the cells can be carried out by various methods. For example, a method of heating eggs to a predetermined temperature (e.g., through a liquid bath, oven, incubator, etc.), exposing the eggs to ammonia and / or other solvents, the eggs to a predetermined temperature Furnace cooling method, the egg contains radio frequency, microwave, infrared radiation, ultra violet radiation, gamma ray radiation, X-ray, etc. However, there are endless ways of applying risk, including but not limited to receiving various electromagnetic waves. Other methods of putting egg cells at risk include laser treatment, cryogenic cooling, and coring. In certain embodiments, radiation treatment, heat treatment, chemical treatment, and weak X-rays may be applied to the entire egg. In other embodiments, ultraviolet light, microwave, heating, laser treatment, cryogenic treatment, mechanical damage, and coring may be used through the window of the egg shells to endanger the embryo. Furthermore, the length and temperature of the storage can be used to endanger the germ leaf cells.

Referring again to FIG. 4, the eggs are then directed to a predetermined (eg, horizontal) arrangement, which causes the air cell to move to a position below the portion facing the top of the egg shell (block 120). ). Then, in accordance with an embodiment of the present invention, the eggs are stored laterally so that the blastocyst can set itself on the side below or near the air cell.

After storage for a period of time, a small opening is formed in the portion facing the top of the egg shell by a punch or other mechanism known to those skilled in the art (block 130). According to an embodiment of the invention, the surface of the eggs, at least around the injection area, can be disinfected (eg with alcohol or other antiseptic solution) to reduce contamination by microorganisms (or others). However, with respect to the embodiment of the present invention, it is not necessary to disinfect the eggs, including the injection area. In addition, a sealant may be applied to the egg shell before forming a small opening in the egg shell, but is not essential. When a seal is applied to the egg shell, the small opening is formed in the egg shell in the area where the seal is located. An opening may also be formed in the outer egg cornea in a step of forming an opening in the egg shell (block 130). In certain embodiments, however, the inner cornea is not affected by the opening of the egg shell and remains essentially intact until the device is inserted through it.

The egg shell opening may be made at any suitable location, for example at the side of the egg adjacent the equator axis or at the end of the egg. In a particular preferred embodiment of the invention, the egg shell opening is introduced in the portion facing the upper side of the egg shell, which is arranged entirely horizontally, and above the air cell which is free to move in the egg. One of ordinary skill in the art will understand that early embryos (eg, blastocysts) will typically set themselves in areas close to or near the top of the egg. Thus the opening of the egg shell is generally in the topmost portion of the egg near where the early embryo (ie, the blastocyst) is expected to settle, unless a means for placing the embryo at another location in the egg is used. Will be made.

The device is then extended into the egg through a small opening in the egg shell (block 140). The device is inserted through the outer cornea and the air cell and penetrates the inner cornea. The device may inject a substance, extract a sample, and / or detect information (block 150). If the device inserted inside the egg is a delivery device, one or more substances may be placed through the delivery device and placed in or near the blastocyst. One or more substances may be put in other positions in the egg. Embodiments of the invention are not limited to the one or more substances put into or near the blastocyst.

If the device to be inserted into the egg is a sampling device, one or more samples (eg, cells of the blastocyst stage) may be removed from or near the blastocyst. One or more samples may be extracted from the outer part of the embryo (eg egg yolk or egg white). For example, samples may be extracted from egg whites to determine whether they are contaminated with microorganisms (eg Salmonella) inside egg whites. The sampling device may be a needle configured to derive a substance (eg, allantoic fluid) from an egg, as would be understood by one of ordinary skill in the art. For example, the needle may have a blunt tip and an axially extending lumen ending in a hole formed adjacent to the tip and in a portion of the needle. Applying a vacuum to the lumen allows material to be drawn into the lumen through the aperture. The blunt tip prevents the lumen from being blocked by the material.

Typically, a sample is removed (extracted) from eggs to obtain information from the eggs. The sample can be removed, for example, in connection with the method of discriminating the viability and sex of the embryo. To illustrate, a sample comprising cells is removed from the embryo, and the cells are removed (typically from eggs) to detect sex specific sequences or sex chromosomes on chromosomes, as known to those of ordinary skill in the art. Can be analyzed). The sample may also be used for other DNA based assays, eg, to determine if a particular gene or allele of interest exists in the embryo.

In some embodiments, the multi-site injection or sampling device described, for example, in US Pat. No. 6,032,612 may be used. Other exemplary delivery and / or sampling devices include US Pat. No. 5,136,979; U.S. Patents 4,681,063 and 4,903,635; And US Pat. Nos. 4,040,388, 4,469,047, and 4,593,646.

If the device inserted into the egg is a detector, various types of information can be detected from the inside of the egg. The detector may be inserted into a position outside the embryo of the egg (eg egg yolk or air cell). Or the detector can be placed near the embryo as described above. In other embodiments, the detector may be placed in the opacity region or the opacca region of the embryo, or in the subgerminal cavity. The detector may be used to collect information such as the size of the embryo, the location of the embryo, the stage of development of the embryo, and / or any characteristics of the embryo, the sex of the embryo, and / or the viability of the embryo, but the information may be It is not limited. The detection device may obtain information about the descent and the position of the embryo.

The information may be captured by equipment (e.g., a computer or other information processor) connected to the detector. Various detectors may be used, including, but not limited to, electrical sensors, optical sensors, chemical sensors, temperature sensors, acoustic sensors, pressure sensors, or other sensors to detect physical or chemical parameters. Exemplary detectors are described in US Pat. No. 6,244,214 to Hebrank.

After material injection and / or sample removal and / or information detection have been performed on the egg, the device is removed from the egg (block 160). The small openings of the egg shells may be sealed by a sealant (block 170) and the eggs may be incubated until hatched (block 180).

One of ordinary skill in the art will understand that the method of the present invention can be performed on multiple eggs, for example in a commercial poultry workshop. In addition, the method described herein may be fully manual, fully automatic, or semi-automatic.

In the following examples, the accelerated egg is referred to as an "SMAC" egg as described above so that the inner egg shell and the outer egg cornea are separated to allow the air cell to move within the egg. SMAC stands for "side manipulated air cell."

Example 1:

As a group of eggs with aircells manipulated as described above, ie SMAC eggs, eggs laid upright (as is common in the nursery industry) showed a hatching rate of 79.7% as described below. The first treatment group, an SMAC with air bubbles, includes eggs with side manipulated air cells as having an inner egg shell ruptured during acceleration to move the air cell. This creates an air bubble in the egg white that is clearly visible when the egg is lit by a candle. The second treatment group, a group of SMAC eggs with a 5/8 inch window, had a 5/8 inch diameter hole drilled in the egg side above the side manipulation aircell. The window was then sealed before setting for hatching. The third group, SMAC, was only manipulated by moving the aircell, the last group being the control group.

This result shows that the SMAC egg group with the inner cornea remaining intact hatches similarly to that of the control group.

The group of incubated SMAC eggs, with the optimal seal, upright (as is common in the nursery industry), exhibited a hatching rate of 88.6% as illustrated below. The first two treatment groups, SMACs with optimal seals and SMACs with poor seals, had windows to make a 5/8 inch diameter hole in the egg side on the side operation aircell. The first group was sealed with a nonporous seal that prevented water loss through the barrier. The second group was sealed with a porous seal that allows excessive moisture loss.

This result indicates that when nonporous seals are used, SMAC treatment with 5/8 inch diameter holes does not result in significantly reduced hatching. However, porous seals cause very markedly reduced hatching.

Example 2 :

SMAC eggs have better visibility of blastocysts through the inner cornea than non-SMAC eggs (egg formed with blunt ends of eggs). In the SMAC egg group, almost all eggs within that group can be injected because of the visibility of the blastocyst. For eggs that are not SMAC eggs, the visibility is poor and cannot be injected or the blastocyst is too far from the window and a significant number of eggs are rejected. SMAC eggs generally have a better location of the blastocyst compared to the eggs whose blunt ends are stored upwards with windows formed at the blunt ends of the eggs. Four different treatment groups were graded based on the visibility of the blastocyst under the endometrial cornea. For all four groups, a window was formed to make a 5/8 inch diameter hole over the egg's air cell. Note the blunt groups in which the hole is made at the blunt end of the egg and the SMAC group in which the air cell is relocated to the side of the egg. A 1 or 'bad' rating indicates that the blastocyst is not visible at all. A grade of 2 or 'normal' indicates that the blastocyst can be seen only by slowly shaking the egg. A rating of 3 or 'good' indicates that the exact location of the blastocyst is visible but its underlying structure cannot be determined in detail. A rating of 4 or 'excellent' indicates that the blastocysts and distant parts, such as the zona pellucida and opaka areas, are clearly visible.

This result indicates that two SMAC groups have much better blastocyst visibility than the clump group. This shows that this method makes it much easier to manipulate the blastocyst compared to the method of forming a window at the blunt end of the egg.

Example 3 :

SMAC eggs were used in the manual injection, the hatching rate of the SMAC injected eggs was 92%. All egg groups, except Group 0 for non-operational control and Group 3 for SMAC non-operational control, had a 5/8 inch diameter window on the egg on the aircell. Groups 1 and 2 had windows made over the aircells at the blunt ends of the eggs. Groups 4 and 5 had windows made on the sides of the eggs above the side engineered air cells. Groups 2 and 5 were injected with phosphate buffered saline and all other groups were not injected.

This result indicates that the SMAC treated groups perform at least as well as or better than the end group blunt with the control group. This indicates that manipulating the air cell as described above has no detrimental effect on developing embryos.

The above description is an illustration of the invention and should not be inferred as limiting the invention. While some embodiments of the invention have been described, those skilled in the art will readily appreciate that many modifications are possible in the exemplary embodiments without departing substantially from the novel teachings and advantages of the invention. Accordingly, all such modifications should be included within the scope of this invention as defined in the claims. The invention is defined by the following claims, including equivalents to those set forth in the claims.

The present invention can be used in the field of egg treatment.

Claims (44)

A method of operating an air cell in a egg at a fixed position between the inner and outer corneas, Accelerating the eggs such that the inner egg shell and the outer egg cornea are separated to allow movement of the air cells in the egg when the eggs are rearranged. The method of claim 1, And said egg is accelerated at an acceleration of at least five times the acceleration of gravity. The method of claim 1, The egg is operated by shaking the egg, characterized in that the operation of the air cell in the egg. The method of claim 1, The egg is a method of operating an air cell in the egg, characterized in that accelerated by frying the egg by hand. The method of claim 1, The egg is accelerated by spinning the egg, characterized in that the operation of the air cell in the egg. The method of claim 1, The egg is a method of operating an air cell in the egg, characterized in that by accelerating the rotation of the egg. The method of claim 1, The egg is a method of operating an air cell in a new egg, characterized in that accelerated by centrifuging the egg. The method of claim 7, wherein And said egg is centrifuged at least five times the acceleration of gravity. A method of treating a new egg comprising an air cell in a fixed position between the inner and outer corneas of the egg, An acceleration step of accelerating the eggs so that the inner egg shell and the outer egg cornea are separated to allow movement of the air cells in the eggs when the eggs are rearranged; Orienting the eggs in a predetermined arrangement such that the air cell is moved to a position below a portion facing the top of the egg shell; An introduction step of introducing a small opening into a portion facing the upper side of the egg shell; Putting a delivery device, a detection device, or a sample extraction device through the small opening of the egg shell and the air cell; A puncturing step of puncturing the inner cornea with the device; And And taking out the device from the egg. The method of claim 9, And the egg is accelerated at an acceleration of at least 5 times the acceleration of gravity. The method of claim 9, Said egg is accelerated by shaking the egg. The method of claim 9, The method of processing a new egg, characterized in that the egg is accelerated by frying the egg by hand. The method of claim 9, Said egg is accelerated by spinning the egg, characterized in that the treatment of birds. The method of claim 9, The egg is a method of treating a bird, characterized in that accelerated by rotating the egg. The method of claim 9, The egg is a method of treating a bird, characterized in that accelerated by centrifuging the egg. The method of claim 15, The method of claim 1, wherein the eggs are centrifuged at least five times the acceleration of gravity. The method of claim 9, The device is a delivery device, further comprising the step of placing the material through the delivery device and placing the material into an egg following the piercing step, wherein the material comprises a group consisting of drugs, nutrients, hormones, nucleic acid molecules, and cells ( The method of processing a bird, characterized in that selected from the group). The method of claim 9, The device is a sample extraction device, the method of processing a bird, characterized in that further comprising the step of removing the sample from the egg following the drilling step. The method of claim 9, And said device is a detection device, further comprising the step of detecting information from the inside of said egg with said detection device following said drilling step. The method of claim 9, And the device is inserted into a predetermined position through the small opening of the egg shell. The method of claim 9, The egg comprises a blastocyst and the device A method of treating a egg, characterized in that it is put through the small opening of the shell of the egg into a position near or adjacent to the blastocyst. The method of claim 9, Before or after the introduction step of introducing a small opening into the shell of the egg, the method of treating a new egg, characterized in that it further comprises risking the cells in the egg. The method of claim 22, The risking of the cells in the egg may include heating the egg, cooling the egg, coring the egg, chemically treating the egg, applying microwave energy to the egg, applying the radio frequency energy to the egg, The addition of ultraviolet energy to the egg, the infrared energy to the egg, the gamma ray to the egg, the X-ray to the egg, the storage of the egg for a certain time at a certain temperature, the laser treatment on the egg, the cryogenic temperature to the egg A method of treating a new egg, comprising: a method selected from the group consisting of cooling and sonication of eggs. The method of claim 22, The risking of the cells in the egg is carried out after the introduction of a small opening to the part facing the upper side of the egg shell, characterized in that the method of processing a new egg. The method of claim 22, Risking cells in the egg is performed by the device inserted through the small opening. The method of claim 22, Endangering the cells in the egg comprises the step of endangering the blastocyst in the egg. The method of claim 9, Wherein said egg is oriented in a generally horizontal position and a small opening of said egg shell is introduced into the side facing the upper side of the egg. The method of claim 9, Prior to introducing the small opening into the egg shell, further comprising applying a sealing material to the egg shell, wherein the small opening is introduced into the egg shell at the position where the sealing material is applied. The method of claim 9, The egg is a chicken, turkey, duck, goose, quail, pheasant, parakeets, parrots, cockertoo, cockatil, ostrich, and a method of processing a bird egg, characterized in that selected from the group consisting of eggs. The method of claim 9, After the take-out step, further comprising sealing the small opening of the egg shell. The method of claim 9, After the take-out step, further comprising the step of nursery until the hatching of the eggs. The method of claim 17, And said device is a multiple injection delivery device. The method of claim 18, The method of treating a new egg, characterized in that the sample removed from the egg comprises a liquid of the descent. The method of claim 18, The method of treating a new egg, characterized in that the sample removed from the egg comprises blastocyst cells. The method of claim 19, And said detection device is selected from the group consisting of an electrical sensor, an optical sensor, a chemical sensor, a temperature sensor, an acoustic sensor, and a pressure sensor. A method of operating an air cell at a fixed position between an inner egg shell and an outer egg cornea in the egg containing the blastocyst, An acceleration step of accelerating the eggs so that the inner egg shell and the outer egg cornea are separated to allow movement of the air cells in the eggs when the eggs are rearranged; Orienting the eggs in a horizontal arrangement such that the air cell is moved to a position below a portion facing the top of the egg shell; And And storing the eggs in a horizontal arrangement for a sufficient time so that the blastocyst is placed close to the air cell itself. The method of claim 36, And said egg is accelerated at an acceleration of at least five times the acceleration of gravity. The method of claim 36, The egg is operated by shaking the egg, characterized in that the operation of the air cell in the egg. The method of claim 36, The egg is a method of operating an air cell in the egg, characterized in that accelerated by frying the egg by hand. The method of claim 36, The egg is accelerated by spinning the egg, characterized in that the operation of the air cell in the egg. The method of claim 36, The egg is a method of operating an air cell in the egg, characterized in that by accelerating the rotation of the egg. The method of claim 36, The egg is a method of operating an air cell in a new egg, characterized in that accelerated by centrifuging the egg. The method of claim 42, And said egg is centrifuged at least five times the acceleration of gravity. delete

Images