KR100798149B1 - Experiment Apparatus for nano particle - Google Patents

Abstract

The present invention relates to a nanoparticle harmfulness test apparatus, and to evaluate the harmfulness of nanoparticles through an inhalation toxicity test that effectively exposes nanoparticles to experimental animals by generating aerosols at a fine nano size. The present invention provides an air compressor (1), a regulator (2), a first HEPA filter (3), an atomizer (4), and a heating dryer ( Heating Dryer (5), Ionizer (6), Dilution Chamber (7), Diffusion Dryer (8), and Nose Only Inhalation Apparatus (9), Condensation Particle Counter (10), Electrostatic Classifier (11), Second HEPA Filter (12), Mass Flow Meter (MFC) This is achieved by providing a flow controller 13, a pump 14, and a dust collector 15.

Nanoparticles, hazard experiment

Description

Nanoparticle Hazard Experiment Device {Experiment Apparatus for nano particle}

1 is a schematic configuration diagram of a nanoparticle hazard test apparatus according to the present invention.

<Description of Symbols for Major Parts of Drawings>

1: Air compressor 2: Regulator

3: first HEPA filter 4: particle generator

5: heating dryer 6: ionizer

7: dilution chamber 8: diffusion dryer

9: non-exposure exposure apparatus 10: condensation particle counter

11: electrostatic granulator 12: second HEPA filter

13 mass flow meter 14 pump

15: dust collector

The present invention relates to a nanoparticle harmfulness test apparatus, and more particularly, to a device for evaluating the harmfulness of nanoparticles through inhalation toxicity experiments that effectively expose nanoparticles to experimental animals by generating aerosols at a fine nano size. .

If the 20th century was a micro era, the 21st century could be referred to as the nano era, and these nano technologies can be broadly classified into nanomaterials, nanodevices, and environmental and biotechnology-based technologies according to their applications.

Here, nanotechnology refers to a technology for creating and manipulating nanometer-level objects, wherein the nanometer means 1 billionth of a meter, that is, about 80,000th of the thickness of hair. This corresponds to the extent of 10 hydrogen atoms side by side.

Accordingly, the nanotechnology artificially manipulates microscopic materials on an atomic or molecular basis to produce materials or devices with new properties and functions, which are known as information technology (IT) and other biotechnology technologies (bio). technology (BT) is being hailed as a cutting-edge technology for realizing BT).

However, nanotechnology, which is regarded as a new technology or a high technology, provides many benefits and benefits that can be recognized as a new technological revolution throughout the industrial field, but it also bears potential risks. As a matter of fact, this potential risk is due to the nature of nanotechnology.

In other words, the smaller the particles, the wider the specific surface area ratio, and the smaller the larger the specific surface area ratio, the higher the toxicity when reacting with biological tissues. For example, some such as titanium dioxide, carbon powder, diesel particles, etc. Nanoparticles have been found to be more toxic, such as causing inflammation as they shrink in size, and have been found in academic studies.

In addition, ultra-fine nanoparticles can be embedded deep into the alveoli or migrate to the brain without being trapped by the airways or mucous membranes. Moreover, recent studies have reported that the accumulation of nanoparticles in the body causes diseases or central nervous system disorders. have.

In addition, since the nanoparticles are too small, it is difficult for human immune cells to detect and extinguish them. For this reason, the nanoparticles may induce cell suicide by applying various stresses, such as causing deformation in DNA. The situation is emerging.

On the other hand, the largest nucleus of the nanoparticles is that when it enters the body, 98% is released and 2% accumulates like heavy metals. The remaining 2% of the nanomaterial is accumulated in each organ in the body.

Therefore, if the nanoparticles accumulated in the body is a toxic substance has a very fatal effect on the human body.

On the other hand, in recent years, as a way to use the nanotechnology more efficiently, a situation that has been developed and used devices that can test the potential harmfulness of nanoparticles.

However, most of the conventional nanoparticle hazard testing apparatuses developed and used for the above uses are expensive imported equipment, and a series of configurations also have very complicated and difficult structural closures. Hazardous test equipment) caused problems such as excessive cost and personnel due to operation and maintenance.

Therefore, the development of nanoparticle harmfulness test apparatus to satisfy both economic efficiency and experimental efficiency through reduced operating cost and personnel remains an urgent task.

Accordingly, the present invention has been devised for evaluating the potential hazards of conventional nanotechnology; According to the present invention, the nanoparticles can be continuously generated for a certain time, and the nanoparticles can be used for inhalation toxicity experiments that can effectively expose the generated nanoparticles to experimental animals, thereby enabling reliable assessment of the harmfulness of the nanoparticles. A particle hazard test apparatus is provided.

On the other hand, another object of the present invention is to provide a nanoparticle harmfulness test apparatus to evaluate the harmfulness of nanoparticles, and to establish a systemic safety standard value through the evaluation results.

Thus, the specific means of the present invention for achieving the above object;

An air compressor which is a means for generating compressed air;

A regulator for adjusting a discharge pressure of the compressed air;

The HEPA filter for filtering the fine dust contained in the compressed air:

Particle generator for generating an aerosol by the filtered compressed air blowing force;

A heating dryer for removing the solvent contained in the aerosol;

An ionizer for ionizing the aerosol from which the solvent is removed;

A dilution chamber for diluting the aerosol by introducing clean air into the neutralized aerosol;

A diffusion dryer for drying the water particles contained in the diluted aerosol;

A non-exposure exposure apparatus for conducting animal experiments by introducing the aerosol dried by the water particles;

A condensation particle counter and an electrostatic granulator coupled to monitor the concentration of the aerosol introduced into the non-exposure apparatus;

A hepa filter for filtering the aerosol passing through the non-exposure apparatus;

A mass flow meter for adjusting the flow rate of the non-floating exposure apparatus;

This is achieved by having a dust collector for filtration and discharge of the aerosol that has passed through the non-coating device.

Hereinafter, a preferred embodiment of the nanoparticle harmfulness experiment apparatus according to the present invention will be described in detail based on the accompanying drawings.

1 is a schematic configuration diagram of a nanoparticle hazard test apparatus according to the present invention, which is an air compressor (1), a regulator (2), and a first HEPA filter (3). ), Particle generator (4), heating dryer (5), ionizer (6), dilution chamber (7), diffusion dryer ( Diffusion Dryer (8), Nose Only Inhalation Apparatus (9), Condensation Particle Counter (10), Electrostatic Classifier (11), Second Hepa A HEPA filter 12, a mass flow controller (MFC) 13, a pump 14, and a dust collector 15 are provided.

Here, the air compressor (1) collectively refers to a machine that allows air to be compressed to a pressure above atmospheric pressure, and thus the air compressor (1) applied to the present invention is a piston reciprocating motion in a cylinder (not shown) and a valve ( It is preferable to apply and use a conventional air compressor as it is, to suck air in accordance with the opening and closing of the drawing, and to compress the sucked air to be discharged to the outside.

In addition, the regulator 2 is a means for adjusting the discharged air through the above-described air compressor 1 to the pressure required for use, such a regulator (2) is composed of a conventional air pressure regulator.

In addition, the first hepa filter 3 and the second hepa filter 12 is a filter for filtering fine dust, which is made of a special fiber used in a clean room, such as a semiconductor company as a material, by a strong adsorption force It consists of a filter that removes mold and mites by filtering 99.9% of fine dust of 0.3 micrometer or more.

In addition, the particle generator (4) is a kind of device for generating an aerosol by spraying a liquid material such as water or chemicals such as mist, which is operated by mechanical external force action such as compressed air, simply by the user's manual It can be classified into two operation modes, such as operating by operation, and in the present invention, it is preferable to use a particle generator operated by the compressed air.

In addition, the heating dryer (5) is a kind of heat source generating means for providing a high temperature hot air, which is a heating wire (not shown) that generates heat by external electrical energy, and discharges the heat generated by the heating wire to the outside Blow fan (not shown) for the basic configuration is made.

In addition, the ionizer 6 is constituted by a conventional device for converting an electrically neutral atom or molecule into a charged atom or molecule (ion).

In addition, the dilution chamber 7 is a means for diluting the aerosol by introducing external clean air, which is formed in a configuration having a certain space shape.

In addition, the diffusion dryer (8) is a kind of drying device to reduce the loss of aerosol by removing water particles, such a diffusion dryer (8) is configured to selectively use a drying agent such as silica gel, charcoal, alinosilicate Will be taken.

In addition, the non-exposure exposure apparatus (9) refers to a conventional toxicity test apparatus, which is a device configuration necessary for the toxicity test of environmental pollutants and the safety confirmation test of other medicines or pesticides, which are experimental animals (rats, moles, etc.). ) Is a general type of device equipped with a feeder (exhaust) (not shown) made of activated carbon and a filter, and other power supply means (not shown). It is composed.

In addition, the condensation particle counter 10 and the electrostatic granulator 11 are coupled to each other to monitor the aerosol concentration in the above-described non-explosion apparatus 9, that is, a conventional scanning mobile particle counter (SMPS: Scanning) Mobility Particle Sizer).

 In addition, the mass flow meter 13 is one of the core components of the semiconductor manufacturing equipment, and is also widely used in various other industrial fields. Thus, the mass flow meter 13 has a function of measuring the flow rate of the present invention. Having a control function at the same time, after arranging a plurality of measuring tubes combined with valves in parallel (not shown), the flow rate is calculated by summing the measured values of the individual measuring tubes when measuring the flow rate. It is configured in such a way that the flow rate through each measuring tube is adjusted by operating a valve (not shown).

In addition, the pump 14 refers to a conventional pump for transporting a liquid or gas through a tube by a pressure action, or for transporting a fluid in a low pressure vessel into a high pressure vessel through the tube. In the case of gas, that is, a pump used for supplying air is applied.

In addition, the dust collector 15 is a kind of air pollution prevention device that filters out harmful particles contained in various particulate matters or factory exhaust gases that cause air pollution. The method is a surface filtration method that attaches dust to the surface of a filter medium such as paper or fiber, and a filter cloth made of a bag-like or flat-shaped filter cloth made of a thick layer such as glass fiber or cotton fiber, that is, a bag filter. Optional configuration of any one of the internal filtration method of collecting dust is preferable.

Thus, in constructing a system by interconnecting the configuration of the present invention as described above;

As shown in FIG. 1, the air compressor 1 has a sequential linkage form, and includes a regulator 2, a first hepa filter 3, a particle generator 4, a heating dryer 5, and the like. , Ionizer 6, dilution chamber 7, diffusion dryer 8, non-explosion apparatus 9, second hepa filter 12, mass flow meter (MFC) Connecting the pump 14 and the dust collector 15 to each other;

Subsequently, the condensation particle counter 10 and the electrostatic granulator 11 are divided into branch lines on the connection line between the non-exposure device 9 and the second HEPA filter 12. Will be implemented.

Thus, look at the relationship of use and interactions of the nanoparticle harmfulness test apparatus according to the present invention constructed as a binding configuration as follows.

First, the compressed air of the air compressor 1 is controlled by the regulator 2 so that the pressure and the discharge amount are regulated, and then the adjusted compressed air is filtered through the first hepa filter 3. To the generator (4).

At this time, the particle generator (4) is provided with a pressure gauge and an orifice (not shown), the injection speed is controlled by the pressure gauge, and the expansion injection action by the orifice (0.5mm in diamter).

In addition, by way of explanation, the particle generator (4) is widely used to generate solid particles such as sodium chloride (Nacl) particles, sugar particles and polystyrene latex (PSL: Polystyrene Latex), and also silicone oil, As a conventional configuration used to generate various vegetable oil particles such as Octyl Phthalte, this particle generator 4 is used to selectively generate experimental particles required for the present invention.

Subsequently, the particle generator 4 generates an aerosol in the form of a solvent including the compressed air according to the delivery of the compressed air. Accordingly, the hot air is generated by using the heating dryer 5 to remove the solvent contained in the aerosol. Remove it.

At this time, the heating dryer (5) is effectively used without being greatly affected by the type of solvent, but when the solvent is made of water, and the flow rate is 5 L / min or less, by applying a diffusion dryer as described later In use, this too does not depart from the scope of the invention.

In addition, the aerosol issued through the particle generator (4) and the heating dryer (5) has a charge, the ion neutralization principle using the ionizer 6, the cation and anion necessary for unbalanced electrostatic pressure Efficiently supplied to minimize loss of aerosol.

In addition, the neutralized aerosol is delivered to the dilution chamber 7 for dilution, and the aerosol delivered as described above is mixed and diluted with external clean air in the dilution chamber 7 and passed through the diffusion dryer 8. Done.

At this time, the diffusion dryer (8) is a desiccant for removing water particles and reducing aerosol loss, and selectively using silica gel, charcoal, alinosilicate, etc., but the flow rate of the desiccant may be 0-4 L /. min, the efficiency is lowered at higher flow rates.

In addition, the aerosol in which the water particles are dried by the diffusion dryer 8 is transferred to the non-exposure exposure apparatus 9 and is introduced to the animal experiment;

In addition, the concentration of the aerosol introduced into the non-explosion device (9) is monitored by a scanning mobile particle measuring device, that is, a condensation particle counter (10) and the electrostatic granulator (11).

In this case, the electrostatic granulator 11 is also referred to as a differential mobility analyzer (DMA), which uses particles neutralized electrically by exposing the polydisperse particles to radiation such as Kr-85 and Po-210. The device sorts by particle size.

In addition, the particles exposed to radiation are electrically charged at a positive rate, negatively charged and neutral depending on the particle size;

Accordingly, the condensation particle counter 10 allows air containing particles to be introduced into a saturator (not shown), which is a component of the condensation particle counter 10, according to a function used for particle number measurement in a range of 0.01 μm to 10 μm. do.

At this time, the saturator is filled with alcohol, the temperature of which is maintained at about 35 ℃, the air containing the particles passing through this saturator is saturated with alcohol, another condensation of maintaining a temperature of 10 ℃ again It flows into the condenser (not shown) which is a structure of the particle counter 10.

Therefore, as the temperature is lowered by the condenser, alcohol vapor is condensed by supersaturation, and condensation of the alcohol is caused by the particles as the core, so that condensation occurs on the wall inside the condenser.

In this way, when condensation occurs on the particles, particles of several tens of nm (nanometer) size grow from several micrometers (micrometers) to several tens of micrometers (micrometers), so that optically grown particles can be easily measured. .

On the other hand, the flow rate of the non-floating exposure device (9) is kept constant by using the mass flow meter (13) and at the same time to supply air using the pump (14).

Finally, the aerosol passing through the non-explosion device 9 is passed through the second hepa filter 12 to the inlet of the dust collector 19, and then filtered by the filter and finally discharged, thereby performing a series of experiments. This is the end.

Therefore, the nanoparticles evaluated for harmfulness through a series of experiments as described above are used as therapeutic means to selectively reach specific parts of the body by applying drugs or antibodies.

delete

As described above, the nanoparticle harmfulness test apparatus according to the present invention generates an aerosol with a fine nano size to evaluate the safety of the nanoparticles through an inhalation toxicity test that effectively exposes the nanoparticles to a test animal. The result is an accurate experimental result that can be set up.

delete

Claims (3)

An air compressor 1 for generating compressed air; A regulator (2) for adjusting a discharge pressure of the compressed air; A first hepa filter 3 for filtering fine dust contained in the compressed air; A particle generator (4) for generating an aerosol by the filtered compressed air blowing force; Heating dryer (5) for removing the solvent contained in the aerosol; An ionizer (6) for ionizing the aerosol from which the solvent is removed; A dilution chamber 7 for introducing clean air into the neutralized aerosol and diluting the aerosol; A diffusion dryer (8) for drying the water particles contained in the diluted aerosol; A non-exposure exposure apparatus (9) for introducing the aerosol dried by the water particles to conduct animal experiments; A condensation particle counter (10) and an electrostatic granulator (11) coupled to monitor the concentration of the aerosol introduced into the non-explosion device (9); A second hepa filter (12) for filtering the aerosol that has passed through the non-coating device (9); A mass flow meter (13) for adjusting the flow rate of the non-floating exposure device (9); And And a dust collector (15) for discharging and discharging the aerosol that has passed through the non-exposure device (9). delete The apparatus of claim 1, wherein the diffusion dryer (8) uses any one of silica gel, charcoal, and aliosilicate as a desiccant.

Images