KR100788755B1 - Apparatus for controlling variation rate of freezing temperature for cryopreserving human adipose tissue and refrigerator equipped the apparatus - Google Patents

Abstract

An apparatus for controlling a variation rate of freezing temperature for cryopreserving a human adipose tissue, and a refrigerator having the same are provided to emit latent heat gradually in a temperature section that moisture or fat in the human adipose tissue forms a freezing point to prevent damage of the human adipose tissue and minimize a change rate of volume due to a rapid temperature change. A first fan(211) supplies external cool air. A heater(220) generates heat. At least one or more first temperature sensors(231) measure a temperature of a human adipose tissue directly or indirectly. A controller(240) operates the first fan to cool the human adipose tissue. If a temperature measured by the first temperature sensors decreases to a preliminarily set temperature section, the controller controls the first fan and the heater to maintain the set temperature section for predetermined time. A casing(250) is installed to mount the first fan, the heater, the first temperature sensors, and the controller.

Description

Freezing temperature change rate control device for freezing and storing human adipose tissue and refrigerator equipped with the device

1 is a view showing a refrigerator equipped with a freezing temperature change rate control apparatus for freezing and storing human adipose tissue according to an embodiment of the present invention.

2 is a view for explaining a refrigeration temperature change rate control apparatus shown in FIG.

3 is a view for explaining the temperature change of the human adipose tissue when the human adipose tissue is frozen in the refrigerator equipped with a freezing temperature change rate control device for freezing and storing the human adipose tissue according to an embodiment of the present invention.

4 is a view for explaining an example in which a refrigerator temperature change rate adjusting device is loaded in the refrigerator in a refrigerator equipped with a freezing temperature change rate adjusting device for freezing and storing human adipose tissue according to an embodiment of the present invention.

<Explanation of symbols for the main parts of the drawings>

100: refrigerator

110: freezer

200: frozen temperature change rate control device

211: first fan 212: second fan

220: heater 221: third fan

231: first temperature sensor 232: second temperature sensor

240: control unit

250: casing

251: The body 252: The opening and shutting part

253: first fan mounting holes 254: second fan mounting holes

261: first barrier 262: second barrier

270: switch

The present invention relates to a freezing temperature change rate control device for freezing and storing human adipose tissue and a refrigerator equipped with the apparatus, and more particularly, to a technique for safely freezing and storing the human adipose tissue containing water and fat. It is related.

Substances collected from living animal bodies such as human tissues, biomaterials, meat, and cells are stored frozen for long-term storage or transportation. However, when the above-mentioned materials are put into the freezer of the existing refrigerator without any action for freezing storage, various problems are generated. This problem is described below.

The cooling objects include moisture. That is, moisture starts to form crystals near 0 ° C (freezing point of water). At this time, in the forming of crystals, only moisture separates and aggregates, thereby causing relatively high osmotic pressure around the crystals. It releases latent heat. In other words, if the temperature drop rate is large in this state (the temperature inside the freezer is very low), latent heat is easily released, and large and characteristic crystals are formed around the crystal nuclei of water. The shape change is severe and the separation rate of the crystal component is increased. In addition, the osmotic pressure around the crystals becomes high, resulting in an unstable state. In addition, due to the crystals thus formed, functional microstructures such as intracellular bodies such as mitochondrial ribosomes, water-containing tissues such as collagen fibers, or cell membranes are damaged.

On the other hand, substances such as human adipose tissue contain a large amount of fat components as well as water, and when the fat forms a crystal, the above-mentioned problems are again derived.

In order to solve these problems, researchers in the field have been cryopreserving the object using cryo supplements such as DMSO (Dimethyl Sulfoxide), glycerol (Glycerol), and is still widely used. However, because the cryopreservatives are toxic, they cannot be used for cryopreservation of human adipose tissue to be injected into the human body.

The present invention has been made to solve the above-described problems, when freezing the human adipose tissue, in the temperature range of the water or fat freezing point contained in the human adipose tissue, so that latent heat release can occur slowly By freezing to prevent the rapid formation of water or fat crystals, to prevent osmotic pressure from increasing around the crystals, and to induce temperature distribution evenly throughout the body's adipose tissue. An object of the present invention is to provide a refrigeration temperature change rate control device and a refrigerator equipped with the device for freezing and storing human adipose tissue which can prevent damage and minimize the rate of change of volume due to rapid temperature change.

In addition, while providing the above-mentioned refrigeration temperature change rate control device, the cold air for freezing the body fat tissue to use a refrigerator provided with a conventional freezer, to produce a new expensive equipment for freezing storage of human fat tissue Another goal is to avoid wasting people, money and time.

Refrigeration temperature change rate control apparatus for freezing the storage of human adipose tissue according to the present invention for achieving the above object, the first fan for supplying external cold air; A heater that generates heat; At least one first temperature sensor for directly or indirectly measuring the temperature of human adipose tissue; Operating the first fan to cool the human body adipose tissue, and when the temperature measured by the first temperature sensor drops to a predetermined specific temperature section, maintaining the specific temperature section for a predetermined time; A control unit controlling one fan and a heater; And a casing in which the first fan, the heater, the at least one first temperature sensor, and the control unit are internally mounted or mounted and open and close to allow the human adipose tissue to enter and exit the body.

Here, the casing is provided with a first fan mounting hole on which one side of the first fan is mounted, and when the operation of the first fan is stopped, inside and outside of the casing through the first fan mounting hole. It is preferable to further include a; first blocking film to block the transmission of air therebetween.

The casing may further include: a second fan having a second fan mounting hole formed at the other side thereof, and discharging cold air supplied to the inside of the casing by the operation of the first fan, and mounted to the second fan mounting hole; And when the operation of the second fan is stopped, the second blocking film to block the transfer of air between the inside and the outside of the casing through the second fan mounting port;

Further, it is preferable that the first fan mounting opening is formed below the casing, and the second fan mounting opening is formed above the casing.

And a second temperature sensor for measuring the air temperature inside the casing, wherein the controller monitors the air temperature inside the casing through the second temperature sensor, when the heater is operated. The heater and the first fan may be controlled to prevent the air temperature inside the casing from leaving the specific temperature section.

On the other hand, the third fan is located adjacent to the heater, causing the convection of the air inside the casing; preferably further comprises a.

The at least one first temperature sensor may indirectly measure the temperature of the human adipose tissue by measuring a temperature change of the reference human adipose tissue contacted by the first temperature sensor.

The control unit may include a temperature section of 0 ° C. to −2 ° C., which is an ice freezing point of moisture contained in the human adipose tissue, and a temperature of −18 ° C. to −20, which is a freezing point section of fat included in the human adipose tissue. In the temperature section of ℃, it is preferable to control the first fan and the heater to maintain each temperature section for a predetermined time.

In addition, the casing is preferably removable inside the vertical storage refrigerator to create a refrigeration environment of -20 ℃ or less.

On the other hand, a refrigerator equipped with a freezing temperature change rate control device for freezing and storing the human adipose tissue according to the present invention for achieving the above object, a refrigerator provided with a freezer compartment for generating cold air; And a detachable inside of a freezer compartment of the refrigerator, receiving cold air from the freezer compartment to cool human adipose tissue, and monitoring the temperature of the human adipose tissue, wherein the temperature of the human adipose tissue includes the human adipose tissue. When the moisture or fat is reaching the freezing point at which crystals start to form, the temperature around the human fat tissue is adjusted so that the temperature of the human adipose tissue maintains a predetermined temperature section before and after the freezing point for a predetermined time. It includes; refrigeration temperature change rate control device.

Here, the freezing temperature change rate control apparatus, the first fan for supplying the cold air of the freezer compartment; A heater that generates heat; At least one first temperature sensor for directly or indirectly measuring the temperature of the human adipose tissue; Operating the first fan to cool the human body adipose tissue, and when the temperature measured by the first temperature sensor drops to a predetermined specific temperature section, maintaining the specific temperature section for a predetermined time; A control unit controlling one fan and a heater; And a casing in which the first fan, the heater, the at least one first temperature sensor, and the controller are internally mounted or mounted and open and close to allow the human adipose tissue to enter and exit the body.

At this time, the casing, the first fan mounting opening to which the first fan is mounted is formed on one side, the refrigeration temperature change rate adjusting device, when the operation of the first fan is stopped, the first fan mounting opening It is preferable to further include; a first blocking film to block the air passing through the inside of the casing and the freezing chamber through each other through.

And, the casing, the second fan mounting opening is formed on the other side, the refrigeration temperature change rate adjusting device, the second fan is mounted while discharging the cold air supplied to the inside of the casing by the operation of the first fan. A second fan mounted to the sphere; And when the operation of the second fan is stopped, the second blocking membrane for blocking the air transfer between the inside of the casing and the freezing chamber through the second fan mounting opening;

In addition, the first fan mounting port is preferably formed on the lower side of the casing, the second fan mounting port is preferably formed on the upper side of the casing.

The refrigerating temperature change rate adjusting device may further include a second temperature sensor configured to measure an air temperature inside the casing, and the controller may monitor the air temperature inside the casing through the second temperature sensor. When the heater is operated, the heater and the first fan may be controlled to prevent the air temperature inside the casing from moving out of the specific temperature section.

On the other hand, the third fan is located adjacent to the heater, causing the convection of the air inside the casing; preferably further comprises a.

The at least one first temperature sensor may indirectly measure the temperature of the human adipose tissue by measuring a temperature change of the reference human adipose tissue that the first temperature sensor contacts.

The control unit may include a temperature section of 0 ° C. to −2 ° C., which is an ice freezing point of moisture contained in the human adipose tissue, and a temperature of −18 ° C. to −20 that is a freezing point of fat included in the human adipose tissue. In the temperature section of ℃, it is preferable to control the first fan and the heater to maintain each temperature section for a predetermined time.

In addition, the refrigeration temperature change rate control device may be operated by receiving power from the refrigerator.

Hereinafter, preferred embodiments will be described so as to understand the present invention described above in more detail.

1 is a view showing a refrigerator equipped with a freezing temperature change rate control apparatus for freezing and storing human adipose tissue according to an embodiment of the present invention. As shown in FIG. 1, a refrigerator equipped with a freezing temperature change rate adjusting device for freezing and storing human adipose tissue according to the present invention includes a refrigerator 100 and a freezing temperature change rate adjusting device 200.

The refrigerator 100 has a freezing compartment 110 that generates cold air.

The refrigeration temperature change rate adjusting device 200 is detachable to the inside of the freezer compartment 110 of the refrigerator 100 and cools the human adipose tissue 1 by receiving cold air from the inside of the freezer compartment 110. ), When the temperature of the human adipose tissue (1) reaches the freezing point at which water or fat contained in the human adipose tissue (1) begins to form crystals, The temperature around the human adipose tissue 1 is adjusted so that the temperature maintains a predetermined temperature section before and after the freezing point for a predetermined time. The refrigeration temperature change rate adjusting device 200, as shown in Figure 2, the first fan 211, the second fan 212, the third fan 221, the heater 220, the first temperature sensor ( 231, a second temperature sensor 232, a controller 240, a first blocking film 261, a second blocking film 262, a switch 270, and a casing 250.

The first fan 211 is provided to supply the cold air of the freezing chamber 110 to the casing 250. That is, although the cold air of the freezer compartment 110 may be supplied into the casing 250 by natural convection, the heat exchange rate may be increased due to the operation of the first fan 211.

The second fan 212 is provided to discharge cold air supplied into the casing 250 by the operation of the first fan 211. The description of the second fan 212 will be described later.

The 3rd fan 221 is provided adjacent to the heater 220 mentioned later, and is the structure which is necessary in order that air in the casing 250 may not be stopped but to generate convection. That is, the configuration is configured to more effectively circulate the cold air supplied through the first fan 211 or to spread the heat generated from the heater 220 evenly throughout the casing 250.

The heater 220 is provided in order to generate heat.

The first temperature sensor 231 directly or indirectly measures the temperature of the human adipose tissue 1, and in this embodiment, the first temperature sensor 231 measures the temperature of the reference human adipose tissue 2, Indirectly measuring the temperature of the human adipose tissue 1 is shown. Of course, depending on the implementation, the body fat tissue (1) to be stored frozen may be directly in contact with the first temperature sensor 231 to measure the temperature.

The second temperature sensor 232 measures the air temperature inside the casing 250.

The controller 240 analyzes the temperature of the human adipose tissue 1 measured by the first temperature sensor 231 and the second temperature sensor 232 or the air temperature inside the casing 250, and accordingly the first fan. 211, the second fan 212 and the heater 220 is controlled. That is, while operating the first fan 211 and the second fan 212 to cool the human body fat tissue 1, the temperature of the body fat tissue 1 measured by the first temperature sensor 231 is set in advance When cooling to a specific temperature section, the operation of the first fan 211 and the second fan 212 is stopped and the heater 220 is operated to maintain the specific temperature section for a predetermined time. Of course, the controller 240 monitors the air temperature inside the casing 250 through the second temperature sensor 232, thereby preventing the temperature of the air inside the casing 250 from being excessively increased due to the operation of the heater 220. The heater 220 is controlled to give, and if necessary, the first fan 211 and the second fan 212 may be operated again to lower the temperature to maintain the specific temperature section. In addition, the third fan 221 may be operated only when the heater 220 is operated by the controller 240 (that is, for convection of heat generated from the heater 220 to the inside of the casing 250. Use), any one of the first fan 211, the second fan 212, the heater 220 may be operated (that is, circulating the cold air supplied through the first fan 211) And circulating the heat of the heater 220).

On the other hand, it is preferable that the first fan 211 and the second fan 212 can be started and stopped at the same time. Because, when only one fan is operated, the pressure inside the casing 250 is biased by positive pressure (when only the first fan 211 is operated) or negative pressure (when only the second fan 212 is operated), Because it can not make a smooth flow of air.

The first blocking film 261 and the second blocking film 262 are opened when the first fan 211 and the second fan 212 are in operation, and the first fan 211 and the second fan 212 are movable. If this is stopped, by closing the first fan mounting holes 253 and the second fan mounting holes 254 formed in the casing 250 to be described later, the air in the casing 250 to the refrigerator 100 The freezing chamber 110 of the interlocking with the air is blocked. Of course, each of the power supply units (not shown) for operating the first blocking film 261 and the second blocking film 262 is further included and controlled by the controller 240 or the first fan 211 or the first fan. It may be installed so that the opening and closing automatically according to the operation of the two fans (212).

In addition, the switch 270 is installed outside the casing 250 to allow the control unit 240 to start or stop the operation. Since the operation of the control unit 240 may be fully automated by programming, It is not.

The casing 250 is designed to be opened and closed so that each of the above components are internally mounted or mounted, to allow the human adipose tissue 1 to enter and exit. The casing 250 of the refrigerating temperature change rate adjusting device 200 shown in FIG. 2 is composed of a main body 251 and a drawer-type opening / closing part 252 so as to be slidable to the side, and a casing 250 of such a structure. 4, there is an advantage in that the space required when the opening and closing portion 252 of the casing 250 facing each other when the loading in the freezer compartment 110, as shown in FIG. Therefore, if you want to take out the human body tissue stored frozen in the freezing temperature change rate control device located at the bottom, there is no inconvenience to lift all the freezing temperature change rate control device located at the top.

In general, the capacity of the refrigerator is determined according to the volume of the internal space. The larger the internal space, the greater the difference in temperature between the points of the internal space. Accordingly, the casing 250 is manufactured in a suitable size so that the temperature difference between the points in the casing 250 does not become large, and is located in the freezer compartment 110 of the refrigerator 100.

In addition, the refrigerator is designed in various ways according to the purpose of use, and the refrigerator used in a general home or business is designed to open and close a door from one side. However, such a refrigerator has a disadvantage in that, when the door is opened, cold air is rapidly released.

Therefore, it is preferable to use the vertical storage refrigerator 100 that can open and close the door from the upper side.

However, even in such a vertical storage refrigerator, there is no loss of cold air when the door is opened. As shown in FIGS. 1 and 4, in the vertical storage type refrigerator, when the door is opened, some of the cold air in the upper side of the freezer compartment 110 is lost, and even if the door is closed again, the side or the bottom of the freezer compartment 110 is closed. Unlike the door, since the evaporator is not built in, the upper temperature inside the freezer compartment 110 becomes higher than the lower side.

These problems include the first fan mounting holes 253 and the second fan mounting holes 254 on which the first fan 211 and the second fan 212 are mounted, respectively, on the lower side and the upper side of the casing 250. By forming, it can complement. That is, when the first fan 211 is operated, relatively low temperature cold air flows into the casing 250 through the first fan mounting hole 253 formed under the casing 250. Cool air is discharged to the upper side of the refrigerator 100 in which the cool air having a relatively high temperature is formed through the second fan mounting hole 254 formed above the casing 250 by the operation of the second fan 212. It can be broken. Therefore, due to the first fan 211 and the second fan 212 for causing convection in the casing 250, an effect of allowing cold air to be evenly distributed throughout the freezer compartment 110 of the refrigerator 100. You can get up to.

On the other hand, the casing 250, the first blocking film 261 and the second blocking film 262 is preferably made of a heat insulating material in order to minimize the mutual conduction of the temperature between the inside and the outside of the casing.

Here, the refrigerator 100 and the freezing temperature change rate adjusting device 200 may be remodeled so that the casing 250 of the freezing temperature change rate adjusting device 200 and the inside of the freezer compartment 110 of the refrigerator 100 may be detached from each other. Although it may be, it should be appreciated that the object of the present invention can be sufficiently achieved by merely placing the refrigerating temperature change rate adjusting device 200 in the freezing compartment 110. At this time, when the refrigeration temperature change rate control device 200 is detachable in the freezer compartment 110, the power connection jack (not shown) so that the power input to the refrigerator 100 can be used by the refrigeration temperature change rate control device 200. It is desirable to be able to be mutually coupled. Of course, the battery 240 may be mounted inside the casing to supply power for operating the control unit 240, the first to third fans 211, 212, 221 and the heater 220 of the refrigeration temperature change rate adjusting device 200. It can also be powered by a cable from the outside.

Hereinafter, the process of freezing the human body fat tissue by using the refrigerator equipped with the above-described freezing temperature change rate control device will be described.

First, a plurality of containers 11 containing the human body adipose tissue 1 are placed on the inside of the freezing temperature change rate adjusting device 200, that is, the opening and closing portion 252 of the casing 250 using the rack 12. After closing and closing the opening and closing portion 252, the refrigeration temperature change rate control device 200 is placed in the freezer compartment 110 of the refrigerator 100 to create a freezing environment of minus 20 ℃.

In addition, the control unit 240 may start the control operation by using the switch 270 installed outside the casing 250. The control unit 240 first operates the first fan 211 and the second fan 212 so that cold air from the lower side of the casing 250 is supplied into the casing 250 to cause convection, and then the second fan 212. ) To escape to the upper side of the casing (250). At this time, the first blocking film 261 and the second blocking film 262 are in an open state. Of course, during the freezing storage method or experiment for freezing, it is possible to adjust the freezing speed according to the research results. That is, by adjusting the rotational speed of the first fan 211 and the second fan 212, the temperature inside the refrigeration temperature change rate adjusting device 200 can be controlled to fall by 1 ℃ per minute or 2 ℃ per minute, such The rotational speeds of the first fan 211 and the second fan 212 may be programmed in advance and input to the controller 240. On the other hand, the air inside the casing 250 may cause more smooth convection by the third fan 221, and thus the inside of the casing 250 may have a uniform temperature distribution. Of course, the third fan 221 is similarly operated when the heater 220 is operated later.

At this time, the first temperature sensor 231 measures the temperature of the reference human adipose tissue 2, thereby indirectly measuring the temperature of the human adipose tissue 1 to be cooled, and controls the measured temperature information. To pass). Here, the reason for the indirect measurement, since the human body adipose tissue (1) to be injected into the human body is usually stored in a closed state, because it is too difficult to measure the temperature by directly contacting the first temperature sensor 231. to be. Therefore, by using the method of using the reference body fat tissue (2) that is the same as the temperature change rate of the human adipose tissue (1) but is not directly injected into the human body, a safer freezing process can proceed.

The second temperature sensor 232 measures the temperature inside the refrigeration temperature change rate adjusting device 200 and transmits the measured temperature information to the controller 240.

The controller 240 analyzes the temperature information input from the first temperature sensor 231 and the second temperature sensor 232. Here, referring to FIG. 3 showing a temperature change process of the human adipose tissue, the human body fat The temperature of the tissue decreases over time, that is, it cools down. When the freezing point of moisture contained in the human adipose tissue reaches 0 ° C (depending on the condition of the human adipose tissue, the freezing point may be different and is approximately 0 ° C to -2 ° C). In addition, moisture forms latent crystals, releasing latent heat (a section in which the latent heat is released while all of the moisture is crystallized is indicated by 'A' in FIG. 3).

At this time, if the temperature inside the casing 250 is too low (for example, −20 ° C.), the latent heat release rate of moisture is rapidly increased. That is, only water is separated and aggregates with each other, causing relatively high osmotic pressure to create an unstable state, and large and characteristic crystals are formed around crystal nuclei of water, and the mechanical shape increases as the crystal size increases. The change is severe and the separation rate of the crystal component is increased. These crystals may damage intracellular bodies such as mitochondrial ribosomes, functional microstructures such as water-containing tissues such as collagen fibers, or cell membranes. Therefore, it is necessary to slow down the latent heat release rate (ie, increase the length of the latent heat release section A), to disperse the osmotic pressure, and to coagulate moisture to prevent large crystals from forming.

In order to slow down the latent heat release rate, the temperature inside the casing 250 should be maintained so that latent heat release occurs slowly, that is, the temperature of the human adipose tissue is maintained at 0 ° C. to −2 ° C. for a long time. Therefore, the operation of the first fan 211 and the second fan 212 is stopped, the first blocking film 261 and the second blocking film 262 are closed, and the heater 220 is operated to form an excessively low temperature. The temperature inside the casing 250 was increased. Of course, only by stopping the operation of the first fan 211 and the second fan 212, the temperature inside the casing 250 is not further lowered or convection occurs so that the length of the latent heat dissipation section A is slightly reduced. Can increase, but this does not provide the perfect warmth. Therefore, by operating the heater 220, it is possible to maintain the temperature inside the casing 250 to 0 ℃ ~ -2 ℃. In this case, the second temperature sensor 232 continuously transmits the temperature information to the control unit 240, and the control unit 240 prevents the temperature inside the casing 250 from becoming excessively high and, if necessary, the first fan again. The temperature of the appropriate section (0 ° C. to −2 ° C.) may be maintained while dropping the temperature again by operating the 211 and the second fan 212.

When the latent heat dissipation period (time) set in the controller 240 has passed, the controller 240 opens the first barrier layer 261 and the second barrier layer 262 again, and the first fan 211 and the second fan. By operating the fan 212 to cause convection, the temperature inside the casing 250 is lowered. Accordingly, as shown in FIG. 3, the temperature of the human adipose tissue 1 is lowered again. Of course, at this time, as set in advance in the control unit 240, by adjusting the speed of the first fan 211 and the second fan 212, it is possible to change the cooling rate.

When the temperature of human adipose tissue reaches -19 ℃, which is the freezing point of fat contained in human adipose tissue (depending on the state of human adipose tissue, the freezing point of fat components may also be different, and it is between -18 ℃ ~ -20 ℃) As the fat forms crystals, it releases latent heat (the area where all fat is crystallized and releases latent heat is denoted as 'B' in FIG. 3).

At this time, as in the case where the water is crystallized, if the temperature inside the casing 250 is too low (when the temperature of the freezing chamber is set to about -40 ° C instead of -20 ° C as in this example), the latent heat release of fat is released. The speed rises sharply, causing problems again that have arisen as moisture rapidly formed crystals.

Therefore, it is necessary to slow down the latent heat release rate of fat contained in human adipose tissue. That is, the operation of the first fan 211 and the second fan 212 is stopped, the first blocking film 261 and the second blocking film 262 are closed, and the heater 220 is operated to form an excessively low temperature. The temperature inside the casing 250 was increased. By operating the heater 220, it is possible to maintain the temperature inside the casing 250 at -18 ° C ~ -20 ° C, by increasing the length of the latent heat release section (B) of fat, the fat could be generated while crystallization The above problems can be solved.

As described above, the detailed description of the present invention has been made by the embodiments with reference to the accompanying drawings, but the above-described embodiments have been described by way of example only, the present invention is limited to the above embodiments. It is not to be understood that the scope of the invention should be understood by the claims and their equivalent concepts.

As described in detail above, according to the present invention, when cooling the human adipose tissue to freeze storage, latent heat release may occur slowly in a temperature section in which water or fat contained in the human adipose tissue forms an ice freezing point. This prevents the formation of crystals rapidly, prevents the osmotic pressure from becoming unstable due to high osmotic pressure around the crystals, and induces even temperature distribution throughout the body's adipose tissue. It can also prevent and minimize the rate of change of volume due to rapid temperature change.

In addition, the freezing temperature change rate adjusting device for freezing and storing the human fat tissue according to the present invention that can control the change rate of the freezing, because the use of cold air generated in the freezer of a common refrigerator, expensive for freezing storage of human fat tissue It is possible to prevent the waste of manpower, cost, and time required to manufacture new equipment.

Claims (19)

A first fan supplying external cold air; A heater that generates heat; At least one first temperature sensor for directly or indirectly measuring the temperature of human adipose tissue; Operating the first fan to cool the human body adipose tissue, and when the temperature measured by the first temperature sensor drops to a predetermined specific temperature section, maintaining the specific temperature section for a predetermined time; A control unit controlling one fan and a heater; And The first fan, the heater, at least one or more first temperature sensor and the control unit is internally or mounted, casing capable of opening and closing so that the human adipose tissue can enter and exit; freezing and storing the human adipose tissue comprising a Refrigeration temperature change rate control device. The method of claim 1, The casing is provided with a first fan mounting opening on which one side of the first fan is mounted, Human adipose tissue, characterized in that it further comprises; when the operation of the first fan is stopped, the first blocking membrane to block the transmission of air between the inside and the outside of the casing through the first fan mounting opening Refrigeration temperature change rate control device for freezing storage. The method of claim 2, The casing, the second fan mounting opening is formed on the other side, A second fan mounted to the second fan mounting hole while discharging cold air supplied into the casing by the operation of the first fan; And When the operation of the second fan is stopped, the second barrier membrane for blocking the transfer of air between the inside and the outside of the casing through the second fan mounting hole; further comprising a human adipose tissue Refrigeration temperature change rate control device for freezing storage. The method of claim 3, The first fan mounting opening is formed on the lower side of the casing, the second fan mounting opening is formed on the upper side of the casing, the freezing temperature change rate control apparatus for freezing storage of human fat tissue. The method of claim 1, Further comprising: a second temperature sensor for measuring the air temperature inside the casing, The control unit monitors the air temperature inside the casing through the second temperature sensor so that when the heater is operated, the air temperature inside the casing is prevented from moving out of the specific temperature section. Refrigeration temperature change rate control device for cryopreservation of human adipose tissue, characterized in that for controlling. The method according to any one of claims 1 to 5, And a third fan positioned adjacent to the heater and causing convection of the air inside the casing. The freezing temperature change rate adjusting apparatus for freezing and storing the human adipose tissue. The method according to any one of claims 1 to 5, The at least one first temperature sensor freezes the human adipose tissue by measuring a temperature change of the reference human adipose tissue contacted by the first temperature sensor, thereby indirectly measuring the temperature of the human adipose tissue. Refrigeration temperature change rate control device for. The method according to any one of claims 1 to 5, The control unit may have a temperature range of 0 ° C. to −2 ° C., which is an ice freezing point of water contained in the human adipose tissue, and a temperature of −18 ° C. to −20 ° C., which is a freezing point of fat included in the human adipose tissue. In the temperature section, refrigeration temperature change rate control apparatus for cryopreserving human fat tissue, characterized in that for controlling the first fan and the heater to maintain each temperature section for a predetermined time. The method according to any one of claims 1 to 5, The casing is a refrigeration temperature change rate control device for freezing storage of human adipose tissue, characterized in that the removable inside the vertical storage refrigerator to create a refrigeration environment of less than -20 ℃. A refrigerator provided with a freezer which generates cold air; And Detachable inside the freezer compartment of the refrigerator, receiving the cold air inside the freezer compartment to cool the human adipose tissue, by monitoring the temperature of the human adipose tissue, the temperature of the human adipose tissue, the human adipose tissue includes When the moisture or fat present reaches the freezing point at which crystals start to form, the temperature around the human adipose tissue is controlled so that the temperature of the human adipose tissue maintains a predetermined temperature section before and after the freezing point for a predetermined time. Refrigeration temperature change rate control device for freezing storage of human adipose tissue, characterized in that it comprises a refrigeration temperature change rate control device. The method of claim 10, The refrigeration temperature change rate adjusting device, A first fan supplying cold air of the freezing compartment; A heater that generates heat; At least one first temperature sensor for directly or indirectly measuring the temperature of the human adipose tissue; Operating the first fan to cool the human body adipose tissue, and when the temperature measured by the first temperature sensor drops to a predetermined specific temperature section, maintaining the specific temperature section for a predetermined time; A control unit controlling one fan and a heater; And The first fan, the heater, at least one or more first temperature sensor and the control unit is internally or mounted, casing capable of opening and closing so that the human adipose tissue can enter and exit; freezing and storing the human adipose tissue comprising a Refrigerator with temperature control for temperature change. The method of claim 11, The casing is provided with a first fan mounting opening on which one side of the first fan is mounted, The refrigeration temperature change rate adjusting device, When the operation of the first fan is stopped, the first blocking membrane for blocking the air passing through the inside of the casing and the freezer compartment through the first fan mounting hole; further comprising a human body fat Refrigerator with freezing temperature change rate control for cryopreserving tissue. The method of claim 12, The casing, the second fan mounting opening is formed on the other side, The refrigeration temperature change rate adjusting device, A second fan mounted to the second fan mounting hole while discharging cold air supplied into the casing by the operation of the first fan; And When the operation of the second fan is stopped, the second blocking membrane to block the air transfer between the inside of the casing and the freezer compartment through the second fan mounting hole; further comprising a human fat Refrigerator with freezing temperature change rate control for cryopreserving tissue. The method of claim 13, The first fan mounting opening is formed on the lower side of the casing, the second fan mounting opening is formed in the upper side of the casing Refrigerator equipped with a freezing temperature change rate adjusting device for freezing storage of human adipose tissue. The method of claim 14, The refrigeration temperature change rate adjusting device, Further comprising: a second temperature sensor for measuring the air temperature inside the casing, The control unit monitors the air temperature inside the casing through the second temperature sensor so that when the heater is operated, the air temperature inside the casing is prevented from moving out of the specific temperature section. Refrigerator equipped with a freezing temperature change rate control device for freezing and storing the human fat tissue, characterized in that for controlling. The method according to any one of claims 11 to 15, And a third fan positioned adjacent to the heater and causing convection of the air inside the casing. 2. The refrigerator equipped with a freezing temperature change rate adjusting device for freezing and storing the human adipose tissue. The method according to any one of claims 11 to 15, The at least one first temperature sensor freezes the human adipose tissue by measuring a temperature change of the reference human adipose tissue contacted by the first temperature sensor, thereby indirectly measuring the temperature of the human adipose tissue. Refrigerator equipped with a temperature control rate of freezing temperature change. The method according to any one of claims 11 to 15, The control unit may have a temperature range of 0 ° C. to −2 ° C., which is an ice freezing point of moisture contained in the human adipose tissue, and a temperature of −18 ° C. to −20 ° C., which is a freezing point of fat included in the human adipose tissue In the temperature section, the refrigerator equipped with a refrigeration temperature change rate control device for freezing storage of human adipose tissue, characterized in that for controlling the first fan and the heater to maintain each temperature section for a predetermined time. The method according to any one of claims 11 to 15, The refrigerator temperature change rate control device is a refrigerator equipped with a temperature change rate control device for freezing storage of human adipose tissue, characterized in that the operation by receiving power from the refrigerator.

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