KR100779499B1 - Micro pipette - Google Patents

Abstract

In a precision pipette for sucking and discharging a certain amount of liquid, an opening portion having a central portion is formed and a receiving portion for receiving a magnetic member in a portion opposite to the opening portion, and a tip mounting portion having a pipette tip detachable at a lower portion thereof, and a piston; A housing accommodating a shaft connected to the piston; A guide member connected to a shaft inside the housing and configured to slide the shaft to adjust the amount of liquid to be sucked, thereby enabling a position movement of a piston connected to the shaft, wherein the guide member protrudes out of the opening. Pressurized handle; A hinge through which the shaft is fastened; A locking piece having a metal member attached thereto to which magnetic force is applied from the magnetic member of the shaft; And a guide piece which is moved along the inner curved surface of the housing when the hinge rotates according to the pressing of the pressing handle.

Pipette, Measuring Vessel, Liquid Suction, Precision, Fine, Eyedropper, Straw

Description

Precision pipettes

1 is a side view showing a conventional pipette;

Figure 2 is an exploded perspective view showing a shaft and the rotation control member in the pipette of Figure 1;

3 is a side view showing a precision pipette according to a first preferred embodiment of the present invention;

4 is an exploded perspective view showing a shaft and a guide part in the precision pipette of FIG. 3;

5 is a cross-sectional view showing the coupling of the housing and the guide portion in the precision pipette of FIG.

Figure 6 is a side cross-sectional view of the coupling of the housing and the guide in the precision pipette of Figure 3;

Fig. 7 is an excerpt view showing a graduation portion provided in the housing and the piston in the precision pipette according to the first modification of the first preferred embodiment of the present invention;

8 is a side cross-sectional view showing the coupling of the housing and the guide portion in the precision pipette according to the second modification of the first preferred embodiment of the present invention; And

9A to 9C are exploded perspective views, recessed front sectional views and recessed side cross-sectional views showing portions A of FIG. 8 in the precision pipette according to the second preferred embodiment of the present invention.

Explanation of symbols on the main parts of the drawings

100: precision pipette 110: gripper

120 housing 121 opening

122: magnetic member 123: receiving portion

124: tip mounting 160: pipette tip

200: guide portion 210: shaft

220: guide block 230: piston

240: division portion 250: separation portion

The present invention relates to a precision pipette, and more particularly, to a precision pipette that can quickly, easily and accurately control the amount of liquid aspirated when a liquid sample is inhaled.

In general, pipettes are side containers used to accurately measure and transport small amounts of liquids, such as chemical experiments, biology, and medicine.

1 is a side view showing a conventional pipette, Figure 2 is an exploded perspective view showing a shaft and a rotation control member in the pipette of FIG.

As shown in FIG. 1 and FIG. 2, the conventional pipette 100 is divided into a grip part 110, a housing 120, and a rotation control member 130. A portion of the central portion of the grip portion 110 is cut and the feed screw 114 is vertically positioned at the cut portion. The feed screw 114 is formed of a male screw thread 114a and screwed to the rotation control member 130. Here, the rotation control member 130 and the feed screw 114 is cut in part so as to have an equal interval, respectively. That is, the cutout portion of the male thread 114a formed on the transfer screw 114 and the portion not cut are equally spaced, and the cutout portion 114b formed on the transfer screw 114 is an arm formed on the rotation control member 130. It has the same area as the uncut portion of the screw thread (130a), the cutout portion 130b formed on the rotation control member 130 has the same area as the male thread (114a) portion of the feed screw (114). Therefore, the rotation control member 130 is also possible to rotate and reciprocate at the same time with respect to the feed screw (114).

In addition, the lower side of the rotation control member 130 is coupled to the housing 120, a guide hole (not shown) is formed in the housing 120 so that a portion of the feed screw 114 can be inserted, the housing 120 At the lower end, a tip mounting part 124 is provided so that the pipette tip 160 can be detached. Here, the inside of the feed screw 114 is penetrated, and the piston 142 connected to the push button 101 is located in the through hole 112.

Therefore, the conventional pipette 100 as described above, by distributing or adjusting the liquid in a predetermined amount using the rotation control member 130, when rotating the rotation control member 130 several times when adjusting the amount of a small range. The housing 120 can adjust the amount to be sucked while going up in the upper direction of the grip portion 110, the female screw thread 130a formed in the feed screw 114 and the rotation control member 130 when adjusting the amount of a large range Each part is cut out so as to have an equal interval, so that the rotation control member 130 is moved in the vertical direction without being rotated.

However, when adjusting the amount of the small range by using the rotation control member 130, the rotation control member 130 is a pipette which is provided in various units, usually 1-10, 20-200, 200-1000 microliters (μl) unit Since one rotation is set to about one-hundredth of the maximum volume to give precision to the, it is difficult to adjust a wide range. For example, in the case of a 200-1000 microliter unit pipette, there is a problem in that the rotation control member 130 needs to be rotated several dozen times in order to adjust the capacity from 200 microliters to 800 microliters.

In addition, when adjusting the amount of a large range using the rotation control member 130, so that the rotation control member 130 is simply moved in the vertical direction so as to be accurately positioned in the range (for example, the desired scale) to be moved. There is no stopping means or fastening means. For example, when a desired amount of liquid is sucked by applying force to the rotation control member 130 in a vertical direction, the housing (there is no gripping force to maintain the force when the pressurized force of the rotation control member 130 is released). 120, the rotation control member 130 is returned to its original state by the internal pressure, there is a problem that it is difficult to suck the exact amount of liquid.

Therefore, in order to be fixed in the position after moving the rotation control member 130 in the vertical direction, there is a hassle to rotate the rotation control member 130 in that position.

In addition, there is a possibility that the fixing method by the rotation control member 130 also loses the gripping force by the force pressed on the rotation control member 130, a new alternative is emerging.

Therefore, an object of the present invention is to combine the guide member with a metal member attached to the piston in the housing in the operation of sucking the liquid sample and to apply the force to the guide member to reciprocate the piston up and down and the magnetic member provided in the housing and The metal member is in close contact with the piston to fix the piston in a desired position, thereby providing a precision pipette that can quickly adjust the amount of liquid sample to be sucked through.

In addition, another object of the present invention is to provide a precision pipette that can accurately determine the amount of the liquid sample sucked during the reciprocating motion of the piston by forming a scale on the housing and the piston.

According to the present invention, in a precision pipette for sucking and discharging a certain amount of liquid, an opening portion having a central portion thereof opened and a receiving portion for receiving a magnetic member in a portion opposite to the opening portion, and a tip at which the pipette tip is detached at a lower end thereof. A housing having a mounting portion for receiving a piston and a shaft connected to the piston; A guide member connected to a shaft inside the housing and configured to slide the shaft to adjust the amount of liquid to be sucked, thereby enabling a position movement of a piston connected to the shaft, wherein the guide member protrudes out of the opening. Pressurized handle; A hinge through which the shaft is fastened; A locking piece having a metal member attached thereto to which magnetic force is applied from the magnetic member of the shaft; And a guide piece which is moved along the inner curved surface of the housing when the hinge rotates according to the pressing of the pressing handle.

In addition, the magnetic member and the metal member is preferably further formed so that the friction means for improving the holding force by the magnetic force interacting with each other in close contact with each other.

In addition, the shaft is preferably formed with a male thread at the center and a fastening hole having a female thread at the other end.

In addition, the hinge of the guide member is preferably formed in the center of the through-hole having a female thread that is screwed with the male thread of the shaft.

In addition, the piston is preferably formed with a fastener having a male thread that is fastened to the female thread formed in the fastening hole of the shaft.

The shaft may further include a guide block moving along a curved surface of the inside of the housing when the guide member is hinged.

In addition, the hinge of the guide member and the shaft is preferably fastened by the insertion through the fixing pin.

In addition, it is preferable that a fixed scale having a scale of a predetermined unit and a moving scale for dividing 19 scales of the fixed scales 20 equally are formed in the housing and the piston, respectively.

In addition, the guide member may include a rotary cam inserted into a semi-cylindrical insertion groove formed in the metal member of the locking piece; A connecting shaft connected to one end of the rotary cam and extending outwardly through an insertion hole formed in a receiving portion of the housing; And a rotation knob which is connected to the connection shaft and rotates so that the rotary cam is discharged from the insertion groove.

Hereinafter, the configuration, operation and effects of the present invention will be described in detail with reference to the accompanying drawings.

In the following description of the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, a detailed description thereof will be omitted.

Figure 3 is a side view showing a precision pipette according to a first embodiment of the present invention, Figure 4 is an exploded perspective view showing a shaft and a guide in the precision pipette of FIG.

As shown in Figure 3 and 4, the precision pipette 100 according to the first embodiment of the present invention can be largely divided into a grip portion 110, a housing 120, a guide portion 200. Here, for a description having the same configuration and operation as the above-described prior art, reference is made to the prior art and the following detailed description of the present invention will be given.

The gripping portion 110 has a volume of about or less that the user can grab by hand.

The housing 120 is coupled to the gripping portion 110, and includes an opening 121 having a central portion of a cylindrical shape opened therein and a receiving portion 123 for receiving the magnetic member 122 at a portion opposite to the opening 121. Is formed and has a tip mounting portion 124 so that the pipette tip 160 can be detached from the lower end.

FIG. 5 is a cross-sectional view illustrating the coupling of the housing and the guide part in the precision pipette of FIG. 3, and FIG. 6 is a side cross-sectional view illustrating the coupling of the housing and the guide part in the precision pipette of FIG. 3.

The guide part 200 includes a shaft 210, a locking block 220, and a piston 230 as shown in FIGS. 4 to 6.

The shaft 210 is inserted into the hollow of the housing 120, and a male thread 212 is formed at a central portion thereof, and a fastening hole 214 having a female thread 213 is formed at the other end thereof.

The locking block 220 is inserted into the hollow through the opening 121 of the housing 120, and the pressing handle 221 protruding out of the opening 121 and the male thread 212 of the shaft 210 penetrate through. Hinge 224 is provided with a through-hole 223 having a female thread 222, the metal member 225 is attached to one end of the magnetic force from the magnetic member 122 accommodated in the receiving portion 123 of the housing 120 The shaft 210 is fastened to the hinge 224 which is closed when the opening 121 is closed and the pressing knob 221 is pressed to prevent contamination from the outside through the locking piece 226 and the opening 121 which are provided with the lock. The guide piece 227 to be rotated stably without shaking with respect to the curved surface inside the housing 120.

The piston 230 has a fastener 232 having a male thread 231 formed at one end thereof to be fastened to the fastening hole 214 of the shaft 210 penetrated by the through hole 223 of the locking block 220. .

Here, reference numeral 211 denotes that the guide block 211 of the shaft 210 has a shaft 210 inside the housing 120 when the locking block 220 rotates together with the guide piece 227 of the locking block 220. It is a guide block to rotate stably without shaking about the curved surface of the.

In addition, the female thread 222 formed in the male thread 212 of the shaft 210 and the through hole 223 of the hinge 224 is mutually screwed when the hinge 224 rotates according to the pressing of the pressure knob 221. It is preferable to be formed to be fastened in a direction opposite to the rotational direction of the hinge 224 to prevent the fastening, the opening (not shown) in the center of the guide piece 227 to facilitate the installation of the locking block 220 C) and penetrate the pressing knob 221 screwed to the hinge 224 in the opening.

Therefore, the guide portion 200, the hinge 224 is inserted so that the locking piece 226 of the locking block 220 is received in the receiving portion 123 of the housing 120, the through hole 223 of the hinge 224 After the male thread 212 of the shaft 210 is fastened to the fastener 232 of the piston 230 is fastened to the fastening hole 214 of the shaft 210.

On the other hand, in the precision pipette of the present invention, the mechanism for extracting the pipette tip 160 will be omitted because it has the same configuration as in the prior art.

Hereinafter, the operation and effects on the precision pipette according to the first preferred embodiment of the present invention will be described.

First, the piston 230 moves downward when the push button 101 is pressed, and the amount of liquid to be sampled when the push button 101 is released is released at the lower end of the pipette tip mounting shaft 124 of the precision pipette 100. It is sucked into a possibly disposable disposable pipette tip 160. In addition, when the push button 101 is pressed, the sampling liquid may be transferred into another container. After such use, the process of extracting the pipette tip 160 from the mounting shaft 124 for the operation of the repetitive precision pipette 100 in aspirating and dispensing new sample fluid and replacing it with a new pipette tip is conventional. Is the same as

Meanwhile, a method for dispensing a liquid in a constant amount or for sampling a specific amount is as follows.

As illustrated in FIG. 6, when the pressing knob 221 of the locking block 220 of the guide part 200 provided in the housing 120 is pressed, the locking piece 226 of the locking block 220 is attached. The metal member 225 loses the magnetic force from the magnetic member 122 accommodated in the receiving portion 123 of the housing 120, so that the metal member 225 corresponds to the pressing direction of the pressing handle 221. Spaced upwards.

Subsequently, as shown in FIG. 5, when the pressure knob 221 of the locking block 220 is slid vertically (horizontally in the drawing) so that a desired amount of liquid is sucked, the piston connected to the shaft 210 is correspondingly corresponding thereto. 230 is moved.

Thereafter, when the pressure knob 221 is released, a magnetic force is transmitted from the magnetic member 122 accommodated in the receiving portion 123 of the housing 120 to the metal member 225 attached to the locking piece 226. The magnetic member 122 and the metal member 225 are in close contact with each other to lock or fix the guide unit 200 at a desired position (or scale) of the user.

Here, the magnetic force of the magnetic member 122 accommodated in the receiving portion 123 of the housing 120 is preferably greater than the pressure inside the housing 120.

In addition, when the pressure inside the housing 120 is greater than the magnetic force of the magnetic member 122, the magnetic member 122 in order to prevent the guide portion 200 which is slid vertically (horizontal in the drawing) is moved from the position. It is preferable that the projections or the threads are further formed to correspond to each other so that the gripping force or the frictional force interacting with each other in a portion in which the metal member 225 is in close contact with each other.

FIG. 7 is an essential part excerpt diagram showing a scale portion provided in the housing and the piston in the precision pipette according to the first modification of the first preferred embodiment of the present invention.

According to a first modified example of the first preferred embodiment of the present invention, as shown in FIG. 7, the fixed scale 241 and the moving scale 242 are formed on the outer side of the housing 120 and the outer side of the piston 230, respectively. The scale unit 240 further includes.

Here, one scale of the fixed scale 241 is formed in units of about 1/10 of the volume (μl) of the precision pipette, and the moving scale 242 is formed to divide the 19 scale of the fixed scale 241 by 20 equal parts. It is desirable to be.

In addition, a portion of the housing 120 in which the scale unit 240 is located may have a transparent color so as to easily check the suction amount measurement of the liquid sample using the fixed scale 241 and the moving scale 242.

That is, when the precision pipette is formed in a unit of 200-1000 microliters (μl), the scale of the fixed scale 241 forms about 80 total scales, and the unit of one scale is 10 microliters (μl). By having a fixed scale 241 and the moving scale 242 can be adjusted to 0.5 microliter (μl) units.

Therefore, through the fixed scale 241 of the housing 120 and the moving scale 242 of the piston 230, it is possible to accurately measure the amount of the liquid sample sucked in the vertical movement of the piston 230.

8 is a side cross-sectional view showing the coupling of the housing and the guide in the precision pipette according to the second modification of the first preferred embodiment of the present invention.

According to a second modification of the first preferred embodiment of the present invention, as shown in FIG. 8, a fixing member such as a riveting or fixing pin for coupling the shaft 210 and the hinge 224 of the locking block 220 ( 228) is further provided.

Therefore, it is possible to prevent the screw coupling from being released when the hinge 210 of the shaft 210 and the locking block 220 which are mutually screwed together rotate the hinge 224 according to the pressing of the pressure knob 221. Here, the male thread 212 and the female thread 222 may not be formed when the shaft 210 and the locking block 220 are coupled by the fixing member 228.

9A to 9C are exploded perspective views, recessed front sectional views and recessed side cross-sectional views showing portions A of FIG. 8 in the precision pipette according to the second preferred embodiment of the present invention.

According to a second preferred embodiment of the present invention, as shown in Figure 9a and 9b, the guide portion 200 is a semi-cylindrical insertion groove formed in the bottom of the metal member 225 of the locking block 220 ( Rotating cam 251 inserted into the 225-1, the connection is connected to one end of the rotary cam 251 and extends to the outside through the insertion through holes (123-1) formed in the receiving portion 123 of the housing 120 It is further connected to the shaft 253 and the connecting shaft 253 further comprises a separation portion 250 consisting of a rotary knob 255 to rotate so that the rotary cam 251 from the insertion groove 225-1.

Hereinafter, the operation and effects on the precision pipette according to the second preferred embodiment of the present invention will be described.

9A and 9B, when the rotary knob 255 of the separation part 250 is rotated, the rotary cam 251 is discharged from the insertion groove 225-1 of the metal member 225, thereby causing the magnetic force. The metal member 225 is spaced apart from the member 122.

Subsequently, as shown in FIG. 5, when the pressure knob 221 of the locking block 220 is slid vertically (horizontally in the drawing) so that a desired amount of liquid is sucked, the piston connected to the shaft 210 is correspondingly corresponding thereto. 230 is moved.

Subsequently, when the rotation knob 255 is rotated in the opposite direction, the rotation cam 251 is inserted into the insertion groove 225-1 of the metal member 225, whereby the magnetic member 122 and the metal member 225 are rotated. In close contact with each other by magnetic force, the user may lock or fix the guide part 200 at a desired position (or scale).

Therefore, according to the present invention, a locking block having a metal member attached to a piston and a shaft inside the housing is coupled, and the piston and the shaft are reciprocated up and down by using the locking block, and the metal using the magnetic force of the magnetic member inside the housing. By bringing the member into close contact with the locking block at a desired position, the amount of liquid sample to be sucked can be adjusted quickly and simply.

In addition, a fixed scale and a moving scale are formed in the housing and the piston, respectively, so that the amount of the liquid sample sucked in accordance with the vertical reciprocating motion of the piston can be accurately measured.

In the above-described invention, specific embodiments have been described, but various modifications may be made without departing from the scope of the invention. Therefore, the scope of the present invention should not be defined by the described embodiments, but by the equivalents of the claims and claims.

As described above, according to the present invention, when adjusting the amount of liquid in the micro unit during the operation of sucking the liquid, the guide member connected to the piston is slid to move in the vertical direction so that the desired amount of liquid is sucked and the magnetic member and the metal member are positioned at the desired position. By locking or fixing the guide member, it is possible to more quickly and simply control the amount of liquid in micro units through a simpler configuration.

In addition, since the fixed scale and the moving scale are formed in the housing and the piston, respectively, the amount of the liquid sample sucked in accordance with the vertical reciprocating motion of the piston can be accurately measured and confirmed.

Claims (10)

A housing having an opening in which a central portion is opened and a receiving portion for receiving a magnetic member in a portion opposite to the opening, and having a tip mounting portion at which a pipette tip is detachable at a lower end thereof, and a piston and a shaft connected to the piston; In a pipette connected to the shaft inside the housing and comprising a guide member for sliding the shaft to adjust the amount of liquid to be sucked to enable the position movement of the piston connected to the shaft, The guide member is A pressing handle protruding out of the opening; A hinge through which the shaft is fastened; A locking piece having a metal member attached thereto to which magnetic force is applied from the magnetic member of the shaft; And Precision pipette, characterized in that it comprises a guide piece which is moved along the inner curved surface of the housing when the hinge is rotated according to the pressing of the pressing handle. The precision pipette according to claim 1, wherein the magnetic member and the metal member are further formed such that friction means for improving the gripping force by magnetic forces interacting with each other in close contact with each other. The precision pipette according to claim 1, wherein the shaft has a male screw thread formed at a central portion thereof and a fastening hole having a female screw thread formed at the other end thereof. The precision pipette according to claim 3, wherein the hinge of the guide member has a through hole having a female thread that is screwed with the male thread of the shaft at a central portion thereof. The precision pipette according to claim 3, wherein the piston has a fastener having a male thread that is fastened to a female thread formed in a fastening hole of the shaft. The precision pipette of claim 3, wherein the shaft further comprises a guide block moving along a curved surface of the inside of the housing when the guide member is hinged. The precision pipette according to claim 4, wherein the hinge and the shaft of the guide member are fastened by a through insertion to the fixing pin. The precision pipette according to claim 1, further comprising a fixed scale having a scale of a predetermined unit in the housing and the piston, and a moving ruler formed with the fixed scale and the moving scale. The precision pipette according to claim 8, wherein the moving scale of the moving scale is formed with a moving scale dividing 19 scales of the fixed scale by 20 equal parts. The method of claim 7, wherein the guide member A rotary cam inserted into a semi-cylindrical insertion groove formed in the metal member of the locking piece; A connecting shaft connected to one end of the rotary cam and extending outwardly through an insertion hole formed in a receiving portion of the housing; And The precision pipette is connected to the connection shaft further comprises a rotary knob for rotating so that the rotary cam is discharged from the insertion groove.

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