KR100729207B1 - Light radiator for cell stimulation - Google Patents

Abstract

A light radiator for cell stimulation is provided to minimize heat generation from a light source and selectively radiate a certain wavelength by using a light emitting diode, regulate a distance from a sample and radiation intensity minutely, and apply various light sources to the sample. The light radiator for cell stimulation comprises: a body(15) forming a certain volume of darkroom(10); an opening/closing door(20) for opening/closing one side of the body; a measuring portion in the body; and a control portion(30) for controlling operation of the measuring portion in the bottom of the body, wherein the measuring portion contains four sample-holding support bars(28) for forming a hexahedron shape, a sample-holding portion(22) having a center penetrating radiation portion and located on the support bars, a light source-mounting plate(47) under the support bars, a radiation distance-regulating bolt(49) for regulating the height of light source-mounting plate located along the sample-holding support bars, and a cooling fan(26) for absorbing heat from the light source-mounting plate; the sample-holding portion contains a photo/electronic device at the same height of the testing cell for analyzing light energy radiated to the sample in real-time; and the light radiator further contains a communication port for measuring the measured light energy by an outer computer through the control portion in real-time.

Description

Light irradiator for cell stimulation {LIGHT RADIATOR FOR CELL STIMULATION}

1 is a schematic configuration diagram of a light irradiator for cell stimulation according to an embodiment of the present invention,

Figure 2 is a schematic cross-sectional view of the measuring unit in the cell irritation light irradiator according to an embodiment of the present invention,

Figure 3 is a schematic plan view of the measuring unit in the cell irritation light irradiator according to an embodiment of the present invention,

Figure 4a is a plan view of the light source in the cell irradiator for irradiating light according to an embodiment of the present invention,

Figure 4b is a schematic block diagram of a sample seating part in the cell irritation light irradiator according to an embodiment of the present invention,

5 is a block diagram of a control panel in the light irradiator for cell stimulation according to an embodiment of the present invention,

Figure 6a, 6b, 6c is a graph measuring the optical wave source density at the sample seat of the cell irritation light irradiator according to an embodiment of the present invention.

<Description of Symbols for Main Parts of Drawings>

10: darkroom 15: main body

20: opening and closing door 22: sample seat

26: cooling fan 28: sample holding support rod

30: control unit 31: current voltage display unit

32: current adjusting bolt 33: voltage adjusting bolt

34: power switch 35: timer

36: time adjustment button 41: irradiation unit

43: light emitting diode (LED) 45: light source panel

47: light source mounting plate 49: irradiation distance adjusting bolt

The present invention relates to a light irradiator for cell stimulation, and more particularly, to minimize the heat generated from the light source in order to test the effect of light energy of a specific wavelength on the growth of cells, precisely the distance and the amount of light with the sample Not only can be adjusted but also relates to a light irradiator for cell stimulation that can be easily tested by replacing various light sources.

When light is irradiated to cells or tissues, the energy level of certain elements is increased, and the cells or tissue units are activated to have physiological results such as promoting cell division, promoting tissue regeneration, and promoting tissue metabolism.

Among these physiological factors, studies by Antonio L.B. Pinheiro show that low-power laser treatments have anti-inflammatory effects, pain relief, and cell growth, and especially these effects are sustained. Among them, the effect on wound healing is different depending on the irradiation time and irradiation mode (CW or PLS) as well as the total amount of light irradiation. During the study, the low power laser of 904nm, 8mW It was reported that light irradiation accelerated the healing of tissues (C. Lucas 1, The Effect of Low Level Laser Therapy (LLLT) on Stage III Decubitus Ulcers (Pressure Sores); a Prospective Randomised Single Blind, Multicentre Pilot Study, Lasers Med Sci) (14) 94-100 (2000)).

Regeneration of wounded tissues has also been studied in which low-power laser treatments have this induction in some degree of damaged cells than in normal cells (Pinheiro, ALB, Nascimento, SC, Vieira, .ALB et al. (2001). ), Effects of LLLT on malignant cells; Study in Vitro.Rechman, P., Fried, D, Haning, P. Lasers in Dentistry Ⅶ, Billingham: SPIE.56-60).

Cell proliferation is determined by wavelength, and low-power lasers influence cell proliferation. In particular, it has been studied that cell proliferation is influenced by wavelength length and irradiation mode (Effect of Wavelength on Low-Intensity Laser Irradiation-). Stimulated Cell Proliferation In Vitro; Lasers in Surgery and Medicine 36: 8-12 (2005)).

However, the conventional light emitting light source using electrical resistance is not only the most effective wavelength required for cell growth because of the emission of ultraviolet light, visible light, infrared radiation and a large amount of heat, but also harmful wavelengths and heat It is difficult to have the reliability of the impact, and the efficiency of their light is also very low in incandescent light bulb is 5%, fluorescent light 27%, there are many problems in applying for medical purposes.

SUMMARY OF THE INVENTION The present invention has been made to solve the above problems, and an object of the present invention is to use a light emitting diode called a cold light source as a light source and not to include other wavelengths including ultraviolet wavelengths, which are harmful to cell growth. It can be irradiated by selecting only the wavelength, and the light efficiency is about 40 ~ 60%, so that it has a relatively high efficiency and provides a high irradiance light irradiator that can obtain high reliability as well as quantitative data.

The present invention provides a cell irritation light irradiator for irradiating light of a predetermined wavelength to the cell, the main body 15 to form a certain volume dark room 10, and the opening and closing door for opening and closing one side of the main body 15 ( 20), a measuring unit accommodated inside the main body 15, and a control unit 30 for controlling the operation of the measuring unit at the bottom of the main body 15, wherein the measuring unit has a hexahedral shape. Two sample seating support rods 28 are mounted, and an upper end of the sample seating support rod 28 is equipped with a sample seating portion 22 through which the irradiating portion 41 is penetrated so that light can be irradiated to the center portion to seat the sample. The light source mounting plate 47 and the height of the light source mounting plate 47 along the sample mounting support rod 28 are irradiated to the lower end of the sample mounting support rod 28 so that light is irradiated through the irradiation part 41. Irradiation distance adjusting bolt 49 and the light source field to be changed It is characterized in that comprising: a cooling fan 26 to draw the heat of the plate 47.

In addition, the control unit 30 of the cell irradiator for irradiating light according to the present invention is a power supply for providing a stable current to the light source panel 45, a timer that can precisely control the time supplied through the power supply The light source panel 45 includes one of a blue light emitting diode (472 nm), a red light emitting diode (647 nm), and an infrared light emitting diode (850 nm), and the center of the selected light emitting diode 43 is formed. -It is formed at equal intervals so that the distance between the center (pitch) is 10mm, dense within 80mm, it is characterized by being configured to integrate light.

On the other hand, the sample seating portion 22 of the cell irradiator for irradiating light according to the present invention is further attached to the light / electric element at the same height as the test cell to analyze the real-time measurement of light energy irradiated to the sample, The light energy measured by the photoelectric device is preferably configured to further include a communication port to enable real-time measurement from an external computer through the control unit 30.

Hereinafter, with reference to the accompanying drawings will be described in detail the light irradiator for cell stimulation of the present invention.

1 is a schematic configuration diagram of a light irradiator for cell stimulation according to an embodiment of the present invention, Figure 2 is a schematic cross-sectional view of the measuring unit in the light irradiator for cell stimulation according to an embodiment of the present invention, 3 is a schematic plan view of the measuring unit in the cell irritation light irradiator according to an embodiment of the present invention, Figure 4a is a plan view of the light source in the cell irradiating light irradiator according to an embodiment of the present invention, 4b is a schematic configuration diagram of a sample seating unit in the cell irritation light irradiator according to an embodiment of the present invention, FIG. 5 is a configuration diagram of a control panel in the cell irradiator light irradiator according to an embodiment of the present invention, and FIG. 6a. , 6b and 6c are graphs of optical power density measured at a sample seat of a cell irradiator for irradiating cells according to an embodiment of the present invention.

Cell irritation light irradiator according to a preferred embodiment of the present invention, as shown in Figures 1 to 5, the main body 15 to form a certain volume dark room 10, and one side of the main body 15 It includes an opening and closing door 20 for opening and closing the control unit, a measuring unit accommodated in the main body 15, and a control unit 30 for controlling the operation of the measuring unit at the bottom of the main body 15.

The main body 15 and the opening and closing door 20 forms a dark room 10 so that external light does not penetrate, and the measurement unit is equipped with four sample seating support rods 28 so as to form a hexahedral shape, and the sample seating support rods ( 28 is mounted on the upper end of the sample receiving portion 22 through which the irradiation portion 41 penetrated so that light can be irradiated to the center portion to seat the sample, and the irradiation portion 41 at the lower end of the sample seating support rod 28. The light source mounting plate 47 and the cooling fan 26 to suck the heat of the light source mounting plate 47 are configured to be irradiated with light through). In addition, the light source mounting plate 47 is connected to the irradiation distance adjusting bolt 49 to change the height of the upper and lower sides along the sample mounting support rod 28. In the present invention, the distance between the light source and the sample can be adjusted to 0 to 150 mm.

The sample seating portion 22 is preferably formed so that the beaker or glass plate containing the sample is stably seated at a predetermined depth along the arc of the irradiating portion 41 on the top surface.

In addition, the light source mounting plate 47 is formed so that the light source panel 45 is seated, and a connector (not shown) is formed to connect the power applied through the control unit 30 to the light source panel 45.

As shown in FIG. 4A, the light source panel 45 uses a light source in which a plurality of light emitting diodes 43 are integrated, and in the present invention, cells are grown to examine the effect of light energy on cell growth. Three kinds of light emitting diodes (472nm), red light emitting diodes (647nm), and infrared light emitting diodes (850nm), which are considered to be important wavelengths, are selected to test the effects of wavelengths from visible to infrared on cell growth. 112 5φ lamp time light emitting diodes 43 were used to effectively radiate a large amount of instantaneous light energy, and the distances between the center and the center of the light emitting diodes 43 were exactly 10 mm. All of these were manufactured in a structure in which light is concentrated by condensing within a diameter of 80 mm.

The control unit 30 preferably has a power supply for providing a stable current of 20 mA, 30 mA to the light source panel 45, and a timer 35 for precisely controlling the time supplied through the power supply. It is configured to include.

As shown in FIG. 5, the power supply includes a current voltage display unit 31, a current adjusting bolt 32 and a voltage adjusting bolt 33 for controlling the current and voltage so as to intuitively recognize the supplied power. And a power switch 34 for controlling the power supply to the power supply, wherein the timer 35 includes a time control button 36 of a starter and a reset, respectively. It is composed.

In addition, the present invention is attached to the optical / electronic device at the same height as the test cell to measure and analyze the energy irradiated to the sample in real time, the control unit is a digital or analog indicator that displays the energy value measured in the optical / electronic device In addition, there is a communication port or data storage and recording device that can analyze it on a computer (not shown).

In the present invention configured as described above, first, the various cells are seated on the sample seat 22 to supply a constant current to irradiate light energy.

At this time, the amount of light reaching the sample receiving unit 22 was measured using an optical meter (Model-1835-C, Newport, USA), and the light source was moved every 5 mm intervals to ensure the reliability of the measurement. In the case of, the optical power intensity (mW / cm ² ) was obtained by measuring more than 5 points on the irradiated surface. For example, when the distance between the light source and the sample is fixed at 50 mm and a current of 30 mA is applied, the optical power intensity is 4.27 mW / cm ² when the blue light emitting diode is used as the light source panel, and the red light is emitted. when using a diode as a light source panel 13.27mW / cm ^2, when using an infrared light emitting diode as a light source panel has been measured in 17.13mW / cm ^2, the experimental data according to each distance, and electric current are provided in Table 1 below And as shown in FIGS. 6A-6C.

Street 0 cm 1 cm 2 cm 3 cm 4 cm 5 cm 6 cm 7 cm 8 cm 9 cm 10 cm 11 cm 12 cm 13 cm 14 cm 15 cm Applied current wavelength Measuring conditions Blue (472nm) Set current: 1.12 A Set voltage: 25.3 V 5.50 5.35 5.18 5.09 4.96 4.81 4.66 4.54 4.43 4.30 4.19 4.09 3.99 3.87 3.77 3.65 40 mA Set current: 1.40 A Set voltage: 28.6 V 5.78 5.64 5.49 5.43 5.32 5.16 5.02 4.89 4.77 4.65 4.54 4.43 4.32 4.21 4.09 3.97 50 mA Red (647nm) Set current: 1.12 A Set voltage: 18.2 V 19.85 18.20 18.00 17.76 17.25 16.60 15.95 15.32 14.65 13.93 13.17 12.35 11.50 10.66 9.92 9.18 40 mA Set current: 1.40 A Set voltage: 20.8 V 22.65 20.90 20.74 20.48 19.92 19.17 18.45 17.66 16.90 16.08 15.18 14.21 13.20 12.30 11.38 10.51 50 mA Infrared (850nm) Set Current: 1.12A Set Voltage: 15.0V 28.73 27.46 25.52 24.32 23.10 21.99 21.07 20.33 19.71 19.18 18.71 18.28 17.84 17.39 16.87 16.29 40 mA Set current: 1.40A Set voltage: 17.3V 35.01 33.45 31.31 29.84 28.34 27.01 25.92 25.01 24.28 23.64 23.08 22.58 22.07 21.49 20.84 20.12 50 mA

On the other hand, the internal circuit of the light source panel 45 is composed of four separate light emitting diodes in a separate circuit in order to supply a stable power supply to each light emitting diode 43, attached a resistor, and the wavelength shift (Wavelength shift) according to temperature The cooling fan 26 operates at the lower end of the light source panel 45 to minimize the time, and the time adjustment is performed up to 99 hours with an accuracy of 1 ms to accurately measure the energy (J / cm ² ) supplied to the sample. By operating the possible timer 35, it was possible to easily experiment with the effect on the cells by providing a quantitative optical power density of a specific wavelength band according to various light emitting sources.

As described above, according to the present invention, by using a light emitting diode called a cold light source, other wavelengths including ultraviolet wavelengths, which are harmful to cell growth, are not accompanied at all, and only specific wavelengths can be selected and irradiated. In addition, the efficiency of light is about 40 ~ 60%, which is relatively higher than other light sources. Especially, it is a system that can measure the energy of light irradiated per unit area and unit time in real time. When conducting research, it is possible to obtain quantitative data with very high reliability.

Claims (3)

In the light irradiator for cell stimulation for irradiating light of a certain wavelength to the cell, The main body 15 forming a dark volume 10 of a constant volume, the opening and closing door 20 for opening and closing one side of the main body 15, the measuring unit accommodated inside the main body 15, and the main body ( A control unit 30 for controlling the operation of the measuring unit at the bottom of the step 15), The measurement unit is equipped with four specimen holding rods 28 to form a hexahedral shape, the irradiation portion 41 is penetrated so that light can be irradiated to the center portion to seat the sample on the upper end of the specimen seating rod 28 A sample seating part 22 is mounted, and a light source mounting plate 47 and a light source along the sample seating support rod 28 are irradiated to the lower end of the sample seating support rod 28 so that light is irradiated through the irradiation part 41. Irradiation distance adjusting bolt 49 and the cooling fan 26 to suck the heat of the light source mounting plate 47 so as to change the height of the mounting plate 47, The sample seat 22 is further attached to the optical / electric element at the same height as the test cell to analyze the light energy irradiated to the sample by measuring in real time, The light irradiator for cell stimulation, characterized in that it further comprises a communication port to be able to measure in real time from the external computer through the control unit 30, the light energy measured in the optical / all devices. The method of claim 1, The controller 30 is configured to include a power supply for providing a stable current to the light source panel 45, and a timer 35 for precisely controlling the time supplied through the power supply, The light source panel 45 is selected from a blue light emitting diode (472 nm), a red light emitting diode (647 nm), and an infrared light emitting diode (850 nm), so that the distance between the center and the center of the selected light emitting diode 43 is determined. A light irradiator for cell stimulation, characterized in that it is formed at equal intervals so as to be 10mm dense within 80mm diameter to accumulate light. delete

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