KR100721179B1 - Composition for weeding comprising extract of plant as an effective ingredient - Google Patents

Abstract

A composition for weed control comprising the extracts of natural plants as an effective ingredient is provided to remove weeds, and minimize environmental pollution by reducing the amount of herbicides used during plant culture. The composition for weed control comprises the extract of at least one natural plant selected from Zephyranthes candida HERB and Lycoris flavescens M. Kim et S. Lee. as an effective ingredient, wherein the natural plant extract is prepared by pulverizing the plant, dipping the pulverized plant in methanol for 5 days, filtering the methanol extract, and concentrating the filtered solution under reduced pressure.

Description

Composition for weeding comprising natural plant extract as an active ingredient {Composition for weeding comprising extract of plant as an effective ingredient}

1 is a photograph showing the results of investigating the weed germination and growth inhibitory effect of Nurinannab extract on the foreskin. At this time, A is the experimental group treated with 1000ppm Nurinupul extract, B is untreated.

Figure 2 is a photograph showing the results of investigating the weed germination and growth inhibitory effect of Nurinannab extract for barley. At this time, A is the experimental group treated with 1000ppm Nurinupul extract, B is untreated.

Figure 3 is a graph showing the growth inhibition effect for each weed of 1,000ppm nurinnae grass extract.

Figure 4 is a graph showing the effect of inhibiting germination and growth of barium by concentration of white flower nano saffron extract.

The present invention relates to a herbicidal composition comprising a natural plant extract as an active ingredient, more specifically, to examine the herbicidal activity against weeds using more than 300 natural plant extracts as a sample, white flowers Nado saffron, Nurinul grass and red It relates to a herbicidal composition comprising a natural plant extract that can be provided by confirming that the yellow herbaceous extract has excellent herbicidal activity.

Recently, the use of pesticides has become a problem globally due to the concern about the soil pollution caused by the excessive use of organic synthetic pesticides, but it is reported that the species of weeds produced in farmland or grassland are becoming more diverse and resistant to some pesticides. Therefore, unlike the desire to reduce the amount of organic synthetic pesticides is becoming more difficult.

In this regard, the Korean government has established separate policies to promote eco-friendly agriculture (1997, 2001), emphasizing the need for natural or biological pesticides, as well as reducing the use of some organic synthetic pesticides that threaten environmental pollution and human health. In addition, the Codex Guidelines on the Production, Processing, Labeling and Distribution of Organic Foods by the International Code Standards Committee (CODEX) also set forth the requirements for acceptable substances used for plant pest control and weed control. The use of herbicides in plant resources is expected to be of increasing importance in future herbicide development strategies, as it must be from minerals.

In particular, plants produce a variety of species of allelopathy, which have chemical defense mechanisms against other plants and organisms in the ecosystem. There are about 20,000 kinds of substances that can be biosynthesized by about 300,000 kinds of plants in this material world, and about 1,500 kinds of substances are extracted or separated from plants every year.

In Korea, various data on the herbicidal activity of plant resources such as native plants, medicinal plants and weeds have been reported, but there have been no cases of developing herbicides using plant-derived natural substances as leading substances in Korea.

In recent years, the use of biotechnology as an eco-friendly pesticide that replaces chemical pesticides has increased the use of 'microbial products' on lawns, and the government is increasingly restricting the use of pesticides in golf courses. Is expected to be even more useful. However, research on the development of natural herbicides in relation to herbicides is still very rare in Korea, and there are no commercialized products for agriculture.

In view of the above, the present inventors have searched for various eco-friendly materials that can be easily obtained around us to select natural herbicides that can replace pesticides and ultimately reduce the amount of herbicide used in plant cultivation. While researching to find an effective control method of the weeds in question, it was confirmed that the outpost extracts of white flower nasapran, nurinnae grass and red yellow creeper have excellent herbicidal activity and came to complete the present invention.

Accordingly, an object of the present invention is to provide a herbicidal composition comprising a natural plant extract as an active ingredient.

The above object of the present invention is made of 300 kinds of natural plant extract as a sample, white germ na saffron, nurinernae grass by investigating herbicidal activity to the representative black plants with excellent germination and early growth in weed and early growth in weeds And by confirming that the outpost extract of red yellow fever has excellent herbicidal activity.

The present invention provides a herbicidal composition comprising a natural plant extract as an active ingredient.

In the present invention, 300 kinds of natural plant extracts are used as samples, and white flowers nado saffron, nurinerum and red yellow are examined by inspecting herbicidal activity with the representative black plants which have excellent germination and early growth among weeds and excellent growth. It is characterized by confirming that the extract of the similarity has excellent herbicidal activity.

Therefore, the natural plant extract in the present invention is characterized in that any one or more selected from the group consisting of white flower nado saffron extract, Nurinupul extract, and red-yellow synergistic extract.

Hereinafter, one specific example of the present invention will be described in detail, but the scope of the present invention is not limited thereto.

Example  1: selection of natural herbicides

The native plants used in this example were 301 points, including 98 kinds of leather trees, and all plant materials were collected directly from the field of Korea in 2000-2001 in cooperation with the Department of Herbal Resources and Resources, Andong National University. Divided by portion and dried at room temperature. The ground mill was immersed in 70% methanol and extracted three times with stirring at intervals of 12 hours for 5 days. The extraction was filtered with Whatman No. 2 filter, the plant residue was removed, and the extract filtrate was concentrated under reduced pressure at 40 占 폚 or lower. The completely dried crude extract was filled with nitrogen gas and stored at -20 ° C and used for herbicidal activity assay.

In order to investigate herbicidal activity, barley and larvae were selected as the representative test plants with excellent germination and early growth, and extracts showing excellent activity against these two weeds were selected. Both barley and larvae were the most common weeds in the field or golf course, and their germination (95% germination within 5 days) and early growth rate were good, making it easy to test herbicidal activity. The treatment concentrations of the native plant extracts were all the same at 1,000 ppm, and after treatment, seed and seaweed seeds were sown in different Petri dishes. The untreated section was treated with distilled water.

As a result of the preliminary investigation of the germination and growth inhibition effect of weeds on the extracts of about 300 native plants as described above, red-yellowing, sintered, succinic acid, and rhinosperm as substances which inhibit the early growth of barley or broadleaf weed Four species were selected, and one species of white flower nado saffron was selected as a material that showed germination inhibition. The herbicidal activity of the five selected extracts was found to be slightly different in the varieties of the seaweed and the larvae. The substances with high inhibitory effects on the growth of the varieties were nurinnaegrass (outpost), 88% of the above-ground growth and 65% of the underground growth Was suppressed and showed the highest value, and growth inhibition rate for the gastric emptying was also 55% (ground part) and 85% (basement part) (Figs. 1 and 2). In the case of gaeboreum, the herbicidal activity of red yellow fever (outpost), fever (outpost) and quarries (outpost) was high. Especially, the outpost extract of red yellow fever was more than 88% (ground) and 95% (underground) of early growth It was investigated to suppress. In addition, the germination of white flower nado saffron (outpost) was very high, 80% and 90%, respectively (Table 1).

Weed Germination and Inhibition of Growth of Natural Plant Extracts (1,000ppm) Whole plant % Inhibition Gastrointestinal wire grass germination Shoot length Root length germination Shoot length Root length No treatment 0 0 0 0 0 0 Lining 30 55 85 20 88 65 Red yellow commercial painting 0 88 95 0 10 87 Similarity 0 80 95 0 5 80 Seoksan 0 76 92 0 11 95 White flower 80 100 75 90 100 98

Example  2: herbicidal effect test

In order to confirm the herbicidal effect, first, Whatman No. 2 was added to Petri dishes, and the extract was adjusted to a concentration of 1,000 ppm. All tests were carried out in the same conditions of 25 ℃ growth chamber (16hr light), the germination rate was examined after 10 days of treatment, shoot length and root length after 20 days. All herbicides were screened in two stages. In the first screening, we selected materials with over 50% of germination of burrows and foreclosures, and above ground and underground growth inhibition. Assay.

We tested herbicide activities of barley, king buckwheat, and ragweed as grasses and weeds, and germination and growth of broadleaf weeds, genus grasses, and moths were selected for broadleaf weeds.

In order to test herbicidal activity against various weeds, the selected five extracts were examined for germination, ground, and subterranean growth of barbaraceae, king breeze, ragweed and broadleaf weeds such as anthill, genus grass, and moth. As a result, it was found that nurinnae grass (outpost), which had a high effect of inhibiting growth, was found to have high inhibitory effect on both the upper and lower parts of the wild barley. (FIG. 3).

The extracts of red-yellowing (outpost), sangsang (outpost) and succinic acid (outpost) were also found to have high herbicidal activity against broadleaf weeds. Was thought to be.

Finally, the white flower na saffron (outpost) showed a difference in the effect of weed seeds. The effect of germination was very high on barley, king breeze, larvae and shamrock, but the effect on the germination of dog grass was rather small. Based on the barley, white flower Nado saffron starch extract was treated by concentration (0ppm, 10ppm, 100ppm, 1000ppm, 3000ppm), and the higher the concentration was, the higher the growth inhibition effect was. Was investigated (FIG. 4). Particularly noteworthy is that the white flower or saffron starch extract exhibits a high growth inhibition of 50% or more against the barley at low concentrations of 3000 ppm. These results suggest that the white flower or saffron starch extract is the most important plant material for the separation of active bodies for the development of pesticide active substances. Was expected to be the material.

As described above, the present invention relates to a herbicidal composition comprising a natural plant extract as an active ingredient, wherein the herbicidal activity against weeds is examined by using about 300 natural plant extracts as a sample. It was confirmed that the outpost extract of red yellow fever had excellent herbicidal activity. Since the extracts have the advantage of being a natural material with excellent herbicidal activity, there is an excellent effect that can provide a herbicidal composition comprising natural plant extracts as an active ingredient without concern about soil contamination due to excessive use of organic synthetic pesticides in the future. It is therefore a very useful invention in the herbicide industry.

Claims (1)

In the herbicidal composition comprising a natural plant extract as an active ingredient, the natural plant extract comprises a natural plant extract, characterized in that any one or more selected from the group consisting of white flower nado saffron extract and red yellow ikebana extract as an active ingredient. Herbicidal composition.

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