KR100721126B1 - Composition for repelling mosquitoes - Google Patents

Abstract

Mosquito repellent composition according to the present invention according to the invention is characterized in that it comprises at least one selected from the group consisting of monoolein, tributyrin, tricaproin, triolein, tripropionine and derivatives thereof do.

Mosquito repellent, monoolein, tributyrin, tricaproin, triolein, tripropionine, mosquitoes, sanitary pest

Description

Mosquito repellent composition {Composition for Repelling Mosquitoes}

Field of invention

The present invention relates to a mosquito repellent. More specifically, the present invention provides a novel moiety comprising monoolein, tributyrin, tricaproin, triolein, tripropionine, derivatives thereof, or mixtures thereof that exhibit excellent repellent activity against mosquitoes. A mosquito repellent composition.

Background of the Invention

In general, various products have been developed to protect against human skin infections or diseases caused by insect attack. In particular, the form of such a product is used to kill insects by directly acting on insects, to suppress insects' habitat or reproduction, or to neutralize insect sensory organs by modifying the direction or cognitive sense of insects.

Of these, the last mentioned method is to use a "repellent" is a method that is particularly well used for mosquitoes. These mosquito repellents do not track the smell or carbon dioxide emitted from the skin of mammals, such as humans, by any mechanism that is not yet clarified, thereby paralyzing the sensory organs of mosquitoes, thereby repelling the mosquitoes.

Mosquitoes are hygienically important because they carry serious diseases in people such as dengue fever, yellow fever, encephalitis and malaria. In one case of malaria, mosquitoes are a very serious sanitary pest, with more than 1 million deaths worldwide each year. In Korea, malaria has recurred since 1993, and the number of malaria cases is increasing every year, causing thousands of malaria patients each year. As such, the direct and indirect damages caused by mosquitoes are enormous.

Mosquito repellents are preferred because they are relatively free from harm or side effects to the human body that occur when pesticides are used. Until now, a variety of mosquito repellents are known. In particular, a substance called N, N-diethyl- m- toluamide (DEET) has been widely used because of its excellent mosquito repellent effect. However, since DEET has an unpleasant odor and high skin penetration, its use is restricted to children, pregnant women, patients with low blood pressure, and those with sensitive skin. It is regulated not to manufacture.

Efforts have been made to replace DEET. Citronella, linalool, and lemongrass as natural products have been known to have mosquito-repelling effects. Mosquito repellents have a much lower mosquito repellent effect than DEET, which raises questions about their practicality.

Accordingly, the present inventors have conducted research to develop a new mosquito repellent derived from natural products, and found that several substances derived from vegetable components have mosquito repelling effects, and have developed a mosquito repellent having excellent repelling effects. will be.

An object of the present invention is to provide a mosquito repellent composition excellent in mosquito repellent effect.

Another object of the present invention is to provide a mosquito repellent composition derived from a plant component and harmless to the human body.

The above and other objects of the present invention can be achieved by the present invention described below.

Summary of the Invention

The mosquito repellent composition according to the present invention is characterized in that it comprises at least one mosquito repellent selected from the group consisting of monoolein, tributyrin, tricaproin, triolein, tripropionine and derivatives thereof.

The mosquito repellent is used at least 1% by weight based on the total composition, preferably at least 2% by weight.

The present invention also includes a method of avoiding mosquitoes by applying the mosquito repellent composition to the skin.

Hereinafter, the content of the present invention will be described in detail.

Detailed Description of the Invention

The mosquito repellent agent in the present invention was found to be a mosquito repellent material from a variety of vegetable fats that can be obtained from nature, monooolein (monoolein), tributyrin (tributyrin), tricaproin, Oleine (triolein), tripropionin (tripropionin) and mixtures thereof were found to have a repelling ability against mosquitoes and applied them.

These monooolein, tributyrin, tricaproin, triolein, tripropionin and mixtures thereof are harmless to humans and very high against mosquitoes. Has repellent activity.

Monoolein used in the present invention is represented by the general formula C ₂₁ H ₄₀ O _{4 It} has a structure of formula (1).

[Formula 1]

Other names of monoolein used in the present invention include glycerol monooleate; 1-Oleoyl-sn-glycerol; 9-Octadecenoic acid (Z)-, monoester with 1,2,3-propanetriol; 9-octadecenoic acid (Z)-, 1,2,3-propanetriol; Glyceryl oleate; Glyceyl oleate is mentioned.

Tributyrin used in the present invention is represented by the general formula C ₁₅ H ₂₆ O ₆ , and has the structure of formula (2).

[Formula 2]

Other names for the tributyrin include Tri-n-butyrin; 1,2,3-propanetriyl tributyrate; Butanoic acid, 1,2,3-propanetriyl ester; Glycerol tributyrate; Glyceryl tributyrate; Tributanoylglycerol can be mentioned.

Tricaproin used in the present invention is represented by the general formula C ₂₁ H ₃₈ O _{6 It} has the structure of formula (3).

[Formula 3]

Other names of the tricaproin include tricaprioylglycerol; 1,2,3-propanetriyl trihexanoate; Tricapriin; Tricaprioylglycerol; Tri-n-caproin is mentioned.

Triolein used in the present invention is represented by the general formula C ₅₇ H ₁₀₄ O _{6 It} has the structure of formula (4).

[Formula 4]

.

.

Other names for the triolein include 9-octadecenoic acid (Z)-, 1,2,3-propanetriyl ester (9-Octadecenoic acid (Z)-, 1,2,3-propanetriyl ester); 1,2,3-propanetriyl tri-((E) -9-octadecenoate) (1,2,3-Propanetriyl tri-((E) -9-octadecenoate)); 9-Octadecenoic acid (Z)-, 1,2,3-propanetriyl ester; 9-octadeceoic acid (Z)-, 1,2,3-propanetriyl ester; Glycerol trioleate; Glyceryl tri (oleate-1-13C); Olein; And trioleoylglycerol.

Tripropionine used in the present invention is represented by the general formula C ₁₂ H ₂₀ O _{6 It} has a structure of formula (5).

[Formula 5]

.

.

Other names of the tripropionine include 1,2,3-propanetriol and tripropanoate (1,2,3-Propanetriol, tripropanoate); 1,2,3-propanetriyl tripropionate; Glyceryl tripropanoate; Tripropanoin; Tripropanoylglycerol; Tripropionylglycerol can be mentioned.

The present invention changes the chemical structure of the above-described monoolein (monoolein), tributyrin (tributyrin), tricaproin (triaproin), triolein (triolein), tripropionin (tripropionin) to high mosquitoes Derivatives for novel repellents that exhibit repellent activity can be prepared and used.

The present invention uses the above-mentioned materials for mosquito repellent alone or by mixing with a known solvent to prepare a mosquito repellent composition. Well known solvents refer to solvents used in conventional repellent compositions or coating materials in the form of creams, liquids, sprays, gels, etc., applied to human skin. In this case, the above-mentioned monoolein, tributyrin, tricaproin, triolein and tripropionine are used individually or in mixture, respectively. In this case, the mosquito repellent composition is used in an amount of 1% by weight or more, preferably 2% by weight or more. When used in less than 1% by weight, it is difficult to obtain sufficient mosquito repelling effect.

The mosquito repellent composition according to the present invention is preferably applied to the skin in the form of a cream, lotion, spray, diffusion agent or coating solvent, and is used where the mosquitoes live. In addition, it is expected to be used as a repellent for arthropods of various species other than mosquitoes.

The present invention will be further illustrated by the following examples, which are merely illustrative of the present invention and are not intended to limit or limit the scope of the present invention.

Example

Sample Preparation

Monoolein, tributyrin, tricaproin, triolein, tripropionine used in the present invention are commercially available or can be directly synthesized, in this embodiment monoolein, tributyrin, tri Olein was purchased from TCI (Glycerol monooleate, Glycerol tri-n-butyrate, and Glycerol trioleat, respectively), and tricaproin and tripropionine were manufactured by Aldrich (Glycerol tripropionat and Glycerol tri, respectively). -n-hexanoat) was purchased and used.

The purity of these materials was confirmed by gas chromatograph (GC) and gas chromatograph-mass spectrometry (GC-MS), and only those having a purity of 99.5 or more were used. Each of these components was made into a 5% solution in 91% isopropyl alcohol (Pharmacopoeia) and used for the mosquito repellent efficacy experiment.

Each of the five samples prepared in this way was tested for the three mosquitoes, namely the forest mosquitoes (Aedes), Cuules (Culex), Anopheles mosquito repellent test.

Test mosquitoes used in the experiment

In this example, three types of black mosquitoes, Aedes aegypti, Culex pipiens pallens, and Anopheles sinensis were used as black mosquitoes. Egyptian forest mosquitoes were sold by the Korea Research Institute of Bioscience and Biotechnology and red house mosquitoes were sold by the Korea Research Institute of Chemical Technology and used after indoor cultivation. The mottled Chinese wing mosquito was collected and used in front of the town hall of Gyehwa-ri, Gyehwa-ri, Buan-gun, Jeollanam-do.

The larvae of these mosquitoes were fed by mixing sifted chicken feed in a 22 × 14 × 7 cm plastic container containing distilled water, and then feeding only fine powder with yeast one to one. After being placed, it was placed in a breeding vessel in which a wire structure of 35 × 35 × 35 cm was meshed, and allegorized as an adult. The adult adult male and female were mixed in a ratio of about 1 to 1 and placed in the same breeding box for 2-3 days to allow mating. This mated 2-3 day old female was used for the repellency assay. For the adult food, 8% sugar water was used soaked in gauze. Breeding conditions were the conditions of light conditions 16: 8 (note: night), temperature 27 ± 2 ℃, relative humidity 80 ± 10%.

Repellent activity assay using skin test

To test the mosquito repelling effect of the prepared samples, 1 ml of sample was evenly applied between the wrists and elbows of healthy adults who voluntarily participated in the test using iron chopsticks. The number of mosquitoes gathered in the arm for 3 minutes was recorded at 30-minute intervals in a container with a net of 50 × 50 × 50 cm acrylic structure. Calculated as time. On both sides and top of the acrylic structure, air and free air was installed by installing a mesh size of 40 cm in length and width. In the front, a hole of size 15 cm in length and length was attached and a long mesh was attached to it to measure repelling activity. It is designed to put the arm into the cage.

Evacuation effect The test was performed only when the bare arms were placed in a container containing mosquitoes to investigate the activity of the mosquitoes used in the test and the bite was confirmed at least twice in the first 3 minutes.

Results of repellent activity of each component

Each sample containing monoolein, tributyrin, tricaproin, triolein and tripropionine was used to assay repellent activity against three mosquitoes according to the method described above. The results are shown in Table 1 and Table 2. In Table 1 and Table 2, Ae is an Egyptian forest mosquito, Cx is a red colony, and An is a speckled Chinese wing mosquito.

In the table above, NA means untested

Table 1 shows the duration of the repellent effect for each material, and records the time until two consecutive bites. Here, the treatment dose of 5% of concentration corresponds to 0.125 mg / cm 2.

In Table 2, the avoidance rate for each time was calculated using Equation 1 below.

[Equation 1]

From the results of Table 1 and Table 2, monoolein, tributyrin, tricaproin, triolein, tripropionine showed a strong repellent activity of 90% or more up to 30 minutes at 0.125 mg / ㎠ treatment level Most of them showed high repelling effect even after 2 hours.

The present invention has the effect of providing a mosquito repellent composition excellent in mosquito repellent effect and harmless to the human body using monoolein, tributyrin, tricaproin, triolein, tripropionine.

Simple modifications and variations of the present invention can be readily used by those skilled in the art, and all such variations or modifications can be considered to be included within the scope of the present invention.

Claims (11)

A mosquito repellent composition comprising at least one mosquito repellent selected from the group consisting of monoolein, tributyrin, tricaproin, triolein, tripropionine and derivatives thereof. The mosquito repellent composition of claim 1, wherein the monoolein has a structure represented by Formula 1 below: [Formula 1]

. The mosquito repellent composition of claim 1, wherein the tributyrin has a structure of Formula 2: [Formula 2]

. The mosquito repellent composition of claim 1, wherein the tricaproin has a structure of Formula 3 below: [Formula 3]

. The mosquito repellent composition of claim 1, wherein the triolein has a structure represented by Formula 4 below: [Formula 4]

. The mosquito repellent composition of claim 1, wherein the tripropionine has a structure represented by Formula 5: [Formula 5]

. The mosquito repellent composition according to any one of claims 1 to 6, wherein the mosquito repellent is used in an amount of 1% by weight or more based on the total composition. 8. The mosquito repellent composition according to claim 7, wherein the mosquito repellent is used in an amount of 2% by weight or more based on the total composition. A method of avoiding mosquitoes comprising applying the mosquito repellent composition of claim 1 to the skin. A method of avoiding mosquitoes comprising applying the mosquito repellent composition of claim 7 to the skin. 10. The method of claim 9, wherein the mosquito repellent composition is in the form of a cream, lotion, spray, diffusing agent or coating agent.