KR100680658B1 - Bio-chip information writing/reading method using magneto-optical type and apparatus - Google Patents

Abstract

According to the present invention, when the laser is scanned so that the temperature of the magnetic film on which the fixed probe is placed can be raised to the Curie temperature, and the magnetic field is applied simultaneously, the magnetization direction of the corresponding position of the fixed probe that is not coupled to the target probe is changed. The present invention relates to a method and apparatus for recording / reproducing biochip information by using a magneto-optical (MO) method for reading the changed magnetization direction and reproducing the biochip information from the magnetization information of the entire biochip. (1) forming a fixed probe on the magnetic film; (2) coupling the target probe coated with the light scattering material on the fixed probe formed on the magnetization film; (3) In the state where the magnetic field is applied to the magnetization film, the laser beam is scanned and the temperature of the scanned point is heated to a temperature higher than the Curie point, and then the laser is turned off to change the magnetization direction at the point where only the fixed probe is present. Causing information to be recorded in the magnetization film; And (4) scanning the laser light on the magnetization film to detect the rotation state of the polarization plane generated according to the magnetic direction of the magnetization film, and reading the recorded biochip information.

Biochip, Scanner, MO, Magnetic Optical, Magnetic field, Magnetic field, Thermomagnetic, Magneto-optical

Description

Bio chip information recording / reproducing method and apparatus using MO method {BIO-CHIP INFORMATION WRITING / READING METHOD USING MAGNETO-OPTICAL TYPE AND APPARATUS}

1 is a diagram illustrating a process for reproducing information of a biochip by scanning a biochip manufactured according to the present invention in an MO method,

2 is a view showing another embodiment of FIG.

3 is a view showing another embodiment of FIG.

4 is a diagram schematically illustrating a configuration of a biochip scanner using an MO method according to the present invention.

*** Explanation of symbols on main parts of drawing ***

100: magnetic film

102, 104: magnetization direction

110: immobilized probe

120: target probe

130: light scattering material (or light shielding material)

140: laser

150: magnetic field generating means

160: MR head (or GMR head)

The present invention relates to a biochip information recording / reproducing method and apparatus using the MO method.

More specifically, when a magnetic field is applied while the temperature of the magnetic film on which the fixed probe is placed is increased to the Curie temperature, the magnetic field is applied to the fixed probe which is not coupled to the target probe according to the direction of the magnetic field. The present invention relates to a biochip information recording / reproducing method and apparatus using an MO method for reproducing the information of the entire biochip by reading the changed magnetization direction of the magnetic film and changing the magnetization direction of the magnetic film.

In general, biochips are also called biological arrays, and the concept has recently been introduced. Biochip is a biological chip such as nucleic acid is immobilized on the substrate (substrate), a well-known biochip is a DNA chip, which can be said that the DNA is fixed on the substrate, the protein chip is a protein fixed on the substrate It can be said.

The basic principle of the biochip is based on the interaction of probe molecules and target molecules immobilized on a very small substrate.

On the other hand, the general method of reading the information of the biochip is a method of detecting the emission level of the fluorescent material contained in the probe molecule is typical laser-induced fluorescence detection (laser-induced fluorescence detection), the laser-induced fluorescence detection method is a fluorescent material This is a floating light source of the absorbing wavelength, and the laser is used to measure the intensity of the fluorescence emitted from the excited state and moved back to the ground state. The concentration can be determined from the intensity of each fluorescence. have. By attaching a fluorescent material to a DNA or protein sample in the same manner as described above can be quantitative analysis.

Among the apparatuses for detecting fluorescence using the laser-induced fluorescence detection method, a confocal laser scanning system is most commonly used. The confocal laser scanning device uses a laser as a light source, receives a fluorescence signal emitted from a sample into a photomultiplier tube, which is a special detector, and converts the digital signal into a digital image.

However, the biochip scanner using the laser-induced fluorescence detection method as described above has a problem in that a precise mechanism for detecting a fluorescence signal and a high sensitivity detection sensor resistant to ambient noise are required because the light collection efficiency and the fluorescence emission efficiency are weak.

The present invention has been made to solve the problems of the prior art as described above, and when the magnetic field is applied to the magnetic film while the fixed probe is placed so that the temperature can rise to the Curie temperature, the magnetic field is applied at the same time. The magnetization direction of the magnetic film placed at the corresponding position of the fixed probe not coupled with the target probe varies according to the direction of the, and the biochip information recording using the MO method to read the changed magnetization direction of the magnetic film and reproduce the information of the entire biochip. A reproduction method and apparatus are provided.

One embodiment of the present invention proposed to solve the above technical problem, in the method for recording / reproducing biochip information, the method comprising the steps of: (1) forming a fixed probe on the magnetization film; (2) coupling the target probe coated with the light scattering material on the fixed probe formed on the magnetization film; (3) In the state where the magnetic field is applied to the magnetization film, the laser beam is scanned and the temperature of the scanned point is heated to a temperature higher than the Curie point, and then the laser is turned off to change the magnetization direction at the point where only the fixed probe is present. Causing information to be recorded in the magnetization film; And (4) scanning the laser light on the magnetization film to detect the rotation state of the polarization plane generated according to the magnetic direction of the magnetization film, and reading the recorded biochip information.

Another embodiment of the present invention provides a method of recording / reproducing biochip information, comprising: (1) forming a fixed probe on an upper portion of a magnetization film; (2) coupling the target probe coated with the light scattering material on the fixed probe formed on the magnetization film; (3) In the state where the magnetic field is applied to the magnetization film, the laser beam is scanned and the temperature of the scanned point is heated to a temperature higher than the Curie point, and then the laser is turned off to change the magnetization direction at the point where only the fixed probe is present. Causing information to be recorded in the magnetization film; And (4) detecting the leakage magnetic field generated from the magnetization film, converting the magnetic field into an electrical signal, and reading the information of the biochip.

In still another aspect of the present invention, there is provided a biochip information recording / reproducing method, comprising: (1) forming a fixed probe on a predetermined substrate; (2) forming a biochip by combining the target probe coated with the light scattering material on the fixed probe; (3) recording the bioinformation of the biochip on an upper portion of the magnetic film whose magnetization direction is initialized using a biochip loading apparatus; (4) The magnetization direction of the magnetic film at the point where only the fixed probe is present is changed by heating the laser light while the magnetic field is applied to the magnetic film, and heating the temperature at the point where the scanned point is above the Curie point. To record information on the bonding state of the target probe of the biochip on the magnetic film; And (5) unloading the biochip and discharging it to the outside, and then scanning laser light onto the magnetic film to detect the rotation state of the polarization plane generated according to the magnetic direction of the magnetization film, and to record the bonding state of the target probe of the recorded biochip. And read the information to reproduce the image on the monitor.

Another embodiment of the present invention provides a device for recording / reproducing biochip information, comprising: a magnetic film, a fixed probe formed on an upper portion of the magnetic film, and a light scattering material coated on the fixed probe; A biochip consisting of a target probe; Magnetic field generating means for applying an external magnetic field matching the magnetization characteristic of the magnetic film; The laser film is scanned while an external magnetic field is applied to the magnetic film to heat the magnetic film at the scanned point to a temperature higher than the Curie point, and then the laser is turned off to change the magnetization direction of the magnetic film at the point where only the fixed probe is present. Laser scanning means for causing the information of the biochip to be recorded in the magnetization film so as to be changed; And reproducing means for scanning laser light to the magnetization film to detect the rotation state of the polarization plane generated according to the magnetic direction of the magnetization film through an analysis means, reading information of the recorded biochip, and outputting the information through the output means. Characterized in that it is provided.

Hereinafter, a biochip using the MO method will be described in detail with reference to the accompanying drawings.

3 is a diagram schematically showing the configuration of a biochip information recording / reproducing apparatus using the MO method according to the present invention.

An apparatus for recording / reproducing biochip information to which the present invention is applied, as shown in FIG. 4, generates an external magnetic field (magnetic field) suitable for the biochip 1 and the magnetic film (100 in FIG. 1) of the biochip 1. When the magnetic field generating means 150 and the magnetic film is applied to the magnetization film 100, the laser beam is scanned and the temperature of the scanned point is heated to a temperature higher than or equal to the Curie point. The laser light reflecting means 145 for changing the magnetization direction of the point so that the information of the biochip is recorded on the magnetic film, and the magnetizing direction of the magnetic film 100 by scanning the laser light having polarization to the magnetic film 100. According to the rotation angle of the polarization plane reflected by the analysis means 175 is configured to read the information of the recorded biochip and the output means 170 for output through the output means 180.

As illustrated in FIG. 1, the biochip 1 is coupled to the magnetic film 100, the fixed probe 110 formed on the magnetic film 100, and the fixed probe 110. It consists of a target probe 120 coated with a light scattering material (or light shielding material). In this case, the light scattering material or the light shielding material applied on the target probe 120 includes a metal component having high reflectance such as gold and silver.

The regeneration means 170 is a laser light scanning means.

On the other hand, the regeneration means 170 may be replaced by a means for detecting the change in the magnetic flux of the leakage magnetic field generated from the magnetic film 100 and converts it into an electrical signal to read the information of the biochip, wherein the regeneration means ( 170 may be an MR head or a GMR head.

A method of recording and reproducing information of a biochip using a biochip scanner using the MO method configured as described above will be described below with reference to the accompanying drawings.

Example 1

1 is a diagram illustrating a process for reproducing information of a biochip by scanning a biochip manufactured according to the present invention in an MO method.

As shown in (a) and (b) of FIG. 1, after the fixed probe 110 is formed on the magnetic film 100, the target probe 120 having light scattering material (or light shielding material) coated thereon. ) Is coupled to the fixed probe 110 formed on the magnetization film 100. In this case, the light scattering material or the light shielding material includes a metal component having high reflectance such as gold and silver.

Then, as shown in (b) of FIG. 1, the laser light reflecting means 145 is driven by applying a suitable magnetic field to the magnetic film 100 by using the magnetic field generating means 150. Is scanned for each of the probes, and the laser is turned off after heating so that the temperature of the magnetic film at the point of injection can rise to a temperature higher than the Curie point.

Then, as shown in (b) of FIG. 1, the magnetization state of the fixed probe 110 coupled with the target probe 120 does not reach the Curie temperature, and thus the magnetization state (spin direction) is not changed. The reason is that the light scattering material or the light shielding material applied on the upper portion of the target probe 120 reflects a part of the laser light, so that the temperature of the magnetic film at the lower side does not reach the Curie point so that the direction of magnetization does not change in the weak magnetic field. Because.

Therefore, the magnetization state at the point where only the fixed probe 110 is not coupled with the target probe 120 is sufficiently transmitted to the magnetic film without the interference of the target probe to reach the Curie temperature, so that the spin direction is changed by the external magnetic field. The state has changed.

When the laser is turned off in the above state, as the magnetic film 100 is cooled, the magnetization is maintained in the direction of the currently changed magnetic field so that the information of the biochip about the coupling state of the target probe and the fixed probe is below the magnetic film 100. ) As it is.

Subsequently, as shown in (c) of FIG. 1, the regeneration unit 170 including the optical scanning device is driven to scan laser light under the magnetic film 100, according to the magnetization direction of the magnetic film 100. By receiving the generated polarization incident and outputting to the analysis means 175, the analysis means 175 detects the rotation state of the polarization plane to read the recorded information of the biochip, and then output through the output means 180 is reproduced by the user The biochip information can be checked on the screen.

At this time, the target probe is coupled to the fixed probe when the rotation state of the polarization plane is rotated in the clockwise direction, and the target probe is fixed to the fixed probe when the rotation state of the polarization plane is rotated in the counterclockwise direction. Unbound

Example 2

FIG. 2 is a diagram illustrating another embodiment of FIG. 1.

As shown in (a) and (b) of FIG. 2, the target probe 120 having the light emitting material (or light shielding material) coated thereon after the fixed probe 110 is formed on the magnetization film 100. ) Is coupled to the fixed probe 110 formed on the magnetization film 100. In this case, the light scattering material or the light shielding material includes a metal component having high reflectance such as gold and silver.

As shown in FIG. 2B, the laser light reflecting means 145 is driven in a state in which a weak magnetic field is applied to the magnetization film 100 using the magnetic field generating means 150. Is scanned for each of the probes and heated so that the temperature of the scanned spot can rise to a temperature higher than the Curie point, and then the laser is turned off.

Then, as shown in (b) of FIG. 2, the magnetization state of the fixed probe 110 coupled to the target probe 120 does not reach the Curie temperature and thus the spin direction is not changed. This is because the light scattering material or the light shielding material applied on the upper portion of the target probe 120 reflects a part of the laser light.

Therefore, the magnetization state at the point where only the fixed probe 110 is not coupled with the target probe 120 reaches the Curie temperature, and the spin direction is changed.

When the laser is turned off in the above state, as the magnetic film 100 is cooled, magnetization is maintained in the direction of the currently changed magnetic field, and information of the biochip is recorded in the magnetic film 100.

Subsequently, as shown in (c) of FIG. 2, the regeneration means 170 including the magneto-resistive (MR) head or the magnetite magneto-resistive (GMR) head is driven to prevent leakage magnetic fields generated from the magnetization film 100. By detecting and converting the signal into an electrical signal and outputting it to the analyzing means 175, the biochip information recorded on the magnetization film 100 is read and then output through the output means 180. Make sure to check.

Example 3

3 is a view showing another embodiment of FIG.

As shown in (a) and (b) of FIG. 3, after the fixed probe 110 is formed on a predetermined substrate, a target probe 120 having a light scattering material (or light shielding material) is applied thereon. It is coupled to the fixed probe 110 to form a biochip. In this case, the light scattering material or the light shielding material includes a metal component having high reflectance such as gold and silver.

Then, as shown in (c) of FIG. 3, bio information of the biochip is loaded by using a biochip loading device, and the magnetic film 100 having the magnetization direction prepared in advance by using the biochip loading device is initialized. The bioinformation is recorded on the medium 200 having the same.

Then, as shown in (d) of FIG. 3, the laser scanning means 145 is driven in a state in which a weak magnetic field is applied to the magnetic film 100 to scan laser light for each probe. Is heated to a temperature higher than the Curie point, the laser is turned off, and the magnetization direction of the magnetic film 100 at the point where only the fixed probe 110 is present is changed to provide information on the bonding state of the target probe 120 of the biochip. To be recorded on the magnetic film 100.

Then, as shown in (d) of FIG. 3, the magnetization state of the fixed probe 110 coupled with the target probe 120 does not reach the Curie temperature, and thus the spin direction is not changed. This is because the light scattering material or the light shielding material applied on the upper portion of the target probe 120 reflects a part of the laser light.

Therefore, the magnetization state at the point where only the fixed pro part 110 is not coupled with the target pro part 120 reaches the Curie temperature, and thus the spin direction is changed.

When the laser is turned off in the above state, as the magnetic film 100 is cooled, magnetization is maintained in the direction of the currently changed magnetic field, and information of the biochip is recorded in the magnetic film 100.

After the biochip information is recorded in the magnetic film 100 as described above, the biochip loading apparatus unloads the biochip and discharges it to the outside, and then scans the laser light onto the magnetic film 100 according to the magnetic direction of the magnetic film. The rotation state of the generated polarization plane is sensed to read information about the coupling state of the recorded target probe 120 of the biochip and reproduced as an image on a monitor.

The present invention is not limited to the above embodiments, and various modifications and changes can be made by those skilled in the art, which are included in the spirit and scope of the present invention included in the appended claims.

The present invention having the above-described configuration and operation and preferred embodiment of the fixed probe is not coupled to the target probe when the external magnetic field is applied while scanning the laser to raise the Curie temperature to the magnetic film located below the fixed probe of the magnetic film. The magnetization direction of the corresponding position is changed, and by reading the changed magnetization direction to reproduce the information of the biochip, there is an effect of realizing low cost and miniaturization of the biochip scanner since no precise mechanism is required.

In addition, the present invention has the effect of providing a structure capable of precise detection while having a simple structure.

In addition, since the present invention can detect and record the information of the biochip at the same time, the recording information itself is the biochip information, and the distribution of the information is excellent and the effect can be stored for a long time.

In addition, the present invention has the effect that the signal detection is clear as compared to the fluorescence signal and the on / off signal is easy to detect when developed as a diagnostic scanner has a great performance.

In addition, the present invention has the effect of enabling the production of high precision / high density biochips.

In addition, the present invention has the effect that can be recycled by removing the probe on the upper portion of the magnetization film to be reused by converting the magnetization direction of the magnetization film in a single direction.

Claims (9)

In the biochip information recording / reproducing method, Using a laser, the magnetic film on which the fixed probe is placed can be scanned to raise the Curie temperature and a magnetic field is applied to the magnetic film in the corresponding position of the fixed probe that is not coupled to the target probe according to the direction of the magnetic field. Making it different; And Reproducing information of the entire biochip by reading the changed magnetization direction of the magnetic film; Biochip information recording / reproducing method using the MO method, characterized in that consisting of. In the method of recording / reproducing biochip information, (1) forming a fixed probe on the magnetic film; (2) coupling the target probe coated with the light scattering material on the fixed probe formed on the magnetic film; (3) The magnetization direction of the magnetic film at the point where only the fixed probe is present is changed by heating the laser light in the state where the magnetic film is applied to the magnetic film and heating the temperature at the point where the scanned point is above the Curie point. To record information on the bonding state of the target probe of the biochip on the magnetic film; And (4) scanning the magnetic film with laser light to detect rotational state of the polarization plane generated according to the magnetic direction of the magnetic film, and reading information on the bonding state of the recorded target probe of the biochip; Biochip information recording / playback method using the MO method, characterized in that consisting of. The method of claim 2, When the rotation state of the polarization plane is rotated clockwise (or counterclockwise), the target probe is coupled to the fixed probe, and the rotation state of the polarization plane is rotated counterclockwise (or clockwise). The biochip biochip information recording / reproducing method using the MO method, characterized in that it can detect that the target probe is not coupled to the fixed probe in the state. In the biochip information recording / reproducing method, (1) forming a fixed probe on the magnetic film; (2) coupling the target probe coated with the light scattering material on the fixed probe formed on the magnetic film; (3) When the magnetic film is applied to the magnetic film, the laser light is scanned to heat the temperature of the magnetic film at the point where it is scanned to a temperature above the Curie point, and then the laser is turned off so that the magnetization direction at the point where only the fixed probe is present is external. Altering the magnetic field so that information on the bonding state of the target probe of the biochip is recorded in the magnetic film; And (4) detecting the leakage magnetic field or the magnetic flux change of the magnetic field generated from the magnetic film and converting it into an electrical signal to read information of the biochip; Biochip information recording / reproducing method using the MO method, characterized in that consisting of. In the method of recording / reproducing biochip information, (1) forming a fixed probe on a predetermined substrate; (2) forming a biochip by combining the target probe coated with the light scattering material on the fixed probe; (3) recording the bioinformation of the biochip on an upper portion of the magnetic film whose magnetization direction is initialized using a biochip loading apparatus; (4) The magnetization direction of the magnetic film at the point where only the fixed probe is present is changed by heating the laser light while the magnetic field is applied to the magnetic film, and heating the temperature at the point where the scanned point is above the Curie point. To record information on the bonding state of the target probe of the biochip on the magnetic film; And (4) Information about the bonding state of the target probe of the biochip recorded by detecting the rotation state of the polarization plane generated according to the magnetic direction of the magnetic film by scanning the laser light on the magnetic film after unloading the biochip and discharging it to the outside Reading and playing the image on a monitor; Biochip information recording / reproducing method using the MO method, characterized in that consisting of. In the biochip information recording / reproducing apparatus, A biochip comprising a magnetic film placed in a thin film form on a substrate, a fixed probe formed on an upper portion of the magnetic film, and a target probe assembly coupled to the fixed probe and coated with a light scattering material thereon; Magnetic field generating means for applying a magnetic field to the magnetic film so that the magnetization of the magnetic film can be changed only above the Curie point temperature; In the state where the magnetic field is applied to the magnetization film, the laser beam is scanned and the temperature of the scanned point is heated to a temperature above the Curie point, and then the laser is turned off to change the magnetization direction of the point where only the fixed probe is present, thereby coupling the target probe of the biochip. Laser light scanning means for recording information on the state in the magnetic film; And Reproducing means for scanning laser light onto the magnetic film to detect a rotation state of the polarization plane generated according to the magnetization direction of the magnetic film through an analysis means, reading information of the recorded biochip and outputting the information through the output means; Biochip information recording / reproducing apparatus using the MO method, characterized in that it comprises a. The method of claim 6, wherein the regeneration means, Biochip information recording / reproducing apparatus using the MO method, characterized in that the laser light scanning means. 6th

The method according to claim 1, wherein the regeneration means, A biochip information recording / reproducing apparatus using the MO method, wherein the magnetic field detects leakage magnetic field or magnetic flux change of the magnetic field and converts the magnetic signal into an electrical signal to read information of the biochip. 8th

The method according to claim 1, wherein the regeneration means, Biochip information recording / reproducing apparatus using the MO method, characterized in that the MR head or GMR head.

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