KR100663080B1 - The bambusae caulis in liquamen water manufacture method for pustular acnes improvement - Google Patents

Abstract

The present invention relates to a method for producing acne improving blood.

Bamboo bustle (Bambusae Caulis in Liquamen) is a product of bamboo bamboo by-products such as bamboo vinegar and bamboo, and the production of bamboo is gradually increasing, so the development of their efficacy and new use is urgently needed. However, at present, commercially available bamboo vinegar-related products have been manufactured in various forms and marketed in various efficacy, but bamboo-related products have not been released, and there is a lack of valid research data on their functionalities. In order to investigate the efficacy of physiological activity, the analysis of general and nutritional components, physical and chemical properties, antioxidant activity in vitro, and HMG-CoA (3-hydroxy-3) in specimens -methylglutaryl CoA) reductase inhibitory activity, In vitro, the effect of improving hypercholesterolemia was confirmed, and acne-causing bacteria are fat-affinity microorganisms, so oily skin is exacerbated by hyperplasia of sebum. Therefore, the inhibitory effect on acne-causing bacteria is investigated by examining the effects of hyperlipidemia and acne. The present invention relates to a method for producing acne improving death water, which has been examined and examined for correlation.

Bamboo water production method, acne improvement

Description

The bambusae caulis in liquamen water manufacture method for pustular acnes improvement}

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1 is a graph showing the weight increase rate of rats bred by administering 6 weeks of death.

Figure 2 is a graph of the hepatic / weight ratio of rats bred for 6 weeks.

Figure 3 is a graph of the dietary efficiency of the rats bred for 6 weeks.

Figure 4 is a graph of the total cholesterol content in serum of rats measured after 6 weeks of administration of varying dose levels (5%, 10%) of high cholesterol diet and death.

5 is a graph of the neutral lipid content in the serum of rats measured after 6 weeks of administration of high cholesterol diet and death dose levels (5%, 10%).

6 is a graph showing the phospholipid content in serum of rats measured after 6 weeks of administration with varying dose levels (5%, 10%) of high cholesterol diet and death.

7 is a graph showing the results of LDL-cholesterol concentration in serum when fed a high-cholesterol diet and high cholesterol diet in rats for 6 weeks.

Figure 8 is a graph of the results on the HDL-cholesterol concentration in serum when fed a high-cholesterol diet and high cholesterol diet in rats for 6 weeks.

Figure 9 is a graph of the results on the HDL-cholesterol / total cholesterol ratio in serum when fed a high-cholesterol diet and high cholesterol diet in rats for 6 weeks.

Figure 10 is a graph of the results on the arteriosclerosis index in serum when fed a high-cholesterol diet and high cholesterol diet in rats for 6 weeks.

Figure 11 is a graph of the ratio of free cholesterol in serum of rats administered with a high cholesterol diet and different concentrations of death.

12 is a graph showing the cholesterol concentration and ratio of cholesteryl esters in rats administered by varying the high cholesterol diet and death concentration.

Figure 13 is a graph of the effect on serum AST activity of rats fed a high cholesterol diet for 6 weeks.

14 is a graph showing the effect of ALT activity in serum of rats fed a high cholesterol diet for 6 weeks.

Figure 15 is a graph of the effect on serum ALP activity of rats fed a high cholesterol diet for 6 weeks.

Figure 16 is a graph of the content of total cholesterol in the liver of rats administered by varying the high cholesterol diet and death concentration.

Figure 17 is a graph of the content of triglycerides in the liver of rats administered by varying the high cholesterol diet and death concentration.

18 is a graph of growth inhibition for S.epidermidis of death.

19 is a graph of growth inhibition for M.furfur of death.

20 is a graph of growth inhibition against P.acnes of death.

Figure 21 is a reference picture showing the improvement of acne rash and purulent condition before and after treatment for 3 months, clinical picture of 5 months treatment.

The present invention relates to a method for producing acne improving blood.

Bamboo bustle (Bambusae Caulis in Liquamen) is a product of bamboo bamboo by-products such as bamboo vinegar and bamboo, and the production of bamboo is gradually increasing, so the development of their efficacy and new use is urgently needed.

Bamboo is called bamboo oil. It is a juice obtained by heating a cotton rod at a high temperature, and the odor is persimmon, cold, poisonous, lively and clear. It has been used for cardiovascular diseases such as consonant, eating, wind, and blood circulation, asthma, bronchial asthma, lowering blood pressure, fever, burns, and fungicides. It is supposed to be taken with Bokryeong, and when taken alone it is prescribed to take a small amount.

Until now, since bamboo is manufactured according to a conventional process, there are many problems such as a small amount of production, uncomfortable work process, unevenness due to quality impurities, and unpleasant odors.

After refining process, color materials such as tar are removed to make it transparent, and phenolic compounds, which are highly hazardous substances, are mostly removed, and edible is possible, but organic acids, phenol derivatives, etc. It is possible to lose a large amount, and modernizes the process of manufacturing the mass production system, which enables the mass production system of products, automation of work process, quality of production and standard uniformity, and hygienic and tar, aldehyde, methanol, carbonyl compound, and carbonate compound. There is an urgent need for the development of an extractor that can produce a large amount of excellent products that are non-toxic and have efficacy and safety obtained through evaluation of efficacy.

In the study of efficacy using death, Sangsu Kim. "The effect of Zhugryug (Zhuli) on the isolated perfused rat heart." Kyung Hee University Doctor Degree Theses, 1998.) , Myocardial relaxation and contractility, and coronary perfusion, etc. were reported. Enzyme activities such as lactic dehydrogenase and creatine phospho kinase, which are known to be elevated during ischemia induction, were significantly inhibited.

Chung Tae Ho .. "Studies on the effects of Chooseok, Jookryeok and their compounds on the blood pressure in rats." Kyung Hee University Master degree theses, 1982. "A study on the effects of Joockrhyuk, Jookrhyuk-Tang and Jookrhyuk-Kangjeup-Tang on antipyretic in pytexic rats." Won Kwong University Master degree theses, 1984. Sa-Hyun, Park, Myung-rae, Cho, Choong-Ryul, Ryu, Woo-suk, Chae .. "Effects of BCL oral adminstration and herbal acupuncture at BL18, BL19 on kiver function changes incuced by alcohol in the mice . "The Journal of Korean Acupuncture & Moxibustion Society 19 (3): 115-125, 2002.) is an oral and medicinal acupuncture agent that has been shown to improve the recovery of harmful alcohol metabolism and liver dysfunction caused by large amounts of alcohol. Reported.

Chapters, etc. (Hae Jin Kim, Seon Min Kim , Young Oh, Ki Sang Jung, Kyoeng Seon Jang .. "Study of physical and chemical characteristics for joockrhyuk (Bambusae Caulis in Liquamen) according to refinement process (Ⅰ)." Korean J. Orental Medical physiology & Pathology, 15 (3) : 473-476, 2001.), has shown that in the experiment to develop a functional beverage for diabetic therapy, the ability to use blood pressure is shown by the fact that death shows a drop in blood sugar without affecting the kidneys and liver. Provided the basis for beverage development.

However, at present, commercially available bamboo vinegar-related products have been manufactured in various forms and marketed in various efficacy, but bamboo-related products have not been released, and there is a lack of valid research data on their functionality.

Hyperlipidemia is an abnormal increase in cholesterol or triglycerides in plasma. Hypercholesterolemia is known to cause atherosclerosis, and hypertriglyceridemia is known to cause pancreatitis. Atherosclerosis is a clinically important problem because it reduces blood flow rate and causes ischemic heart disease, angina pectoris, and myocardial infarction.

Risk factors for cardiovascular disease include genetic factors, westernization of life patterns, increased nutrient intake, increased animal fat intake, weight gain, decreased exercise, increased stress, longer life expectancy, and older population. LDL (cholesterol) has been reported as one of the major factors of hyperlipidemia that causes ischemic heart disease such as arteriosclerosis and cerebrovascular disease.

In order to treat hypercholesterolemia, HMG (3-hydroxy-3-methylglutaryl) -CoA reductase inhibitor that directly inhibits the synthesis of cholesterol and a lot of drugs of the dermal acid derivatives that lower the concentration of triglyceride in the blood have been developed and used. In addition, long-term use of these drugs has been reported to be associated with side effects such as fat-soluble vitamin deficiency, decreased liver function and decreased kidney function.

Therefore, in recent years, lowering the concentration of LDL (Light Density Lipoprotein) cholesterol in the blood based on diets using natural products, herbal medicine, or folk remedies to lower serum lipids reduces the risk of cardiovascular disease. In addition, many studies are being conducted to enable the prevention or treatment of atherosclerosis.

Acne associated with lipid metabolism occurs mainly in puberty, most of which naturally improve in the late teens or early twenties, but in the minority, acne that usually occurs at age 25 is called adult acne. It is divided into acne (persistent acne) lasting from puberty to age 25 or older and late-onset acne after the age of 25 (late-onset acne).

There is a difference in sebum secretion according to the clinically unknown condition or acne, and it has been reported to be correlated with the lipid level (sebum secretion) of acne patients and the number of acne bacteria (Park, JK, Choi). , SW, Che, KO, Koh, JS, Kim, HO and Park, YJ. "A comparison of the sebum excretion rate and the density of Propionibacterium acnes between adult acne and adolscent acne. K. Dermatol. 38 (9) : 1199 -1204, 2000.).

The main factors involved in acne etiology include sebaceous hyperplasia, abnormal follicular keratosis, acne (Propionibacterium acnes) proliferation and inflammation, and other hormonal or immunological factors may be involved.

In particular, sebum stagnated in the hair follicles blocks the hair follicles and blocks the circulation of air, so the anaerobic bacteria resident inside the hair follicles can grow well.

Acne-causing bacteria are anaerobic microorganisms that are anaerobic and grow on the inside of hair follicles, aerobic, hair follicles or follicles, or low-pathogenic, lipophilic, dimorphic fungi, which are found around normal skin hair follicles. Antigens (Malasseziza furfur) and Ptyrosporum ovale grow in the hair follicles and cause inflammation (Leeming, J, P., K THOLLAND, and WJ Culkffe. "The Microbal ecology of pilisevaceous units from humman skin. J. Gen. Microbiol. 130: 803-807, 1984.).

This malassezia folliculitis can also be seen on the face and can also occur in combination with vulgar acne.

In the meantime, death has been used to treat skin diseases and athlete's foot, and recently, the anti-inflammatory, anti-allergic and fatigue recovery effects of death have been reported.

See also, Hoon Jeon. "Effect of Bambusae Caulis in Liquamen on the synthesis of Type IV Collagen and Laminin during proliferation and differen tiation of 3T3-L1 cells." Kor. J. Herbology, 18 (3): 187-194 , 2003.) It has been reported that killing increased the synthesis of collagen and laminin in the basement membrane protein in vitro. Basic research on the possibility of developing a new concept of acne improver is required.

Other food additives are used for preservation of natural fungicides, smoked foods, etc., but systematic research on this is still very poor.

In view of the above points, the present invention intends to propose a method for preparing acne improving death water. It has been used as a folk remedy since ancient times, and the general ingredient, nutritional component analysis, physico-chemical analysis to examine the efficacy of physiological activity with the sample of death which is known to be effective for the prevention and treatment of various diseases in oriental medicine such as Dongbobogam. Properties, antioxidant activity in vitro, HMG-CoA (3-hydroxy-3-methylglutaryl CoA) reductase inhibitory activity in vitro, In vitro (in vivo) to confirm the effect of improving hypercholesterolemia, because acne-causing bacteria are fat-friendly microorganisms, oily skin is worsened by the hyperplasia of sebum, so to determine the effect on the inhibitory action against acne-causing bacteria.

In order to achieve the above object, in order to determine the physiological activity effect, first, as a sample, general component and nutritional component analysis, physical and chemical properties, antioxidant activity in vitro, specimen test (in vitro) ), HMG-CoA (3-hydroxy-3-methyl glutaryl CoA) reductase inhibitory activity, To examine the materials and methods according to the practice for confirming the effect of improving hypercholesterolemia in vitro.

Example 1

Materials and methods

(Experimental materials and instruments)

1. Experimental Materials

end. Manufacture and Purification

In 2003, it was manufactured in Dongjeongja Village, Namsan-ri, Damyang-eup, Damyang-gun, Jeonnam, Korea.
Cutting a 3-4 year-old cotton rod ( Phyllostachys nigra var. Henonis) about 20-30 cm, standing in a spring for 12 hours, and then drying it in the shade to remove the nodes at both ends, followed by vertical division ;
25kg of ingredients are put in a charcoal kiln and the amount of air is controlled and heated to 900 ~ 1000 ℃, and the collected juice is cooled to 80 ~ 150 ℃ by passing cooling water around the communication to recover 1.5L (SG 1.07) liquid. After the second step (recovery rate: 5.6%) in the crucible to mature;
Purified by adding 140 mg of activated carbon (200-250 mesh, Yakuri pure chemical Inc. Japan) to the aged juice solution, and distilled at 108 ° C. using a self-made atmospheric distillation apparatus to obtain 1.2 L (SG 1.00). Extracting purified bamboo force (B);
The distilled water is added to 3 mL of the third stage of the distilled water to prepare 10 mL of the dead water. (Recovery rate: 4.8%).

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2. Laboratory equipment

end. Centrifuge: Centrikon T-324 Kontron, Instrument, Italy

I. Evaporator: Rotary vacuum evaporator

All. Millipore deionitaer: ma 01730 braedford

la. Spectrophotometer: Shimadzu UV-1601pc, Kyoto, Japan

hemp. Shaking water bath: JEIO-TEK SWBO3

bar. Deep freezer: OPR-DFU-250C

four. Rancimat 679: Metrohm Ltd. CH-9101 Herisau Switzerland

Ah. Amino acid autoanalyzer: LKB-4150, Alpha Co., U.S.A

ICP-AES (Inductively Coupled Plasma-Atomic Emission Spectroscopy) Jovin Yvon 138 Ultrace, France

car. Color meter: CM-3500d, Minolta Co., Ltd., Japan

Ka. pH meter: Denver Instrument model 15, USA

(Experimental method)

1. Physicochemical Characterization

end. Physical and chemical properties

Specific gravity was measured in a constant temperature bath (Je10-Tek, SWBO3) at 15 ± 1 ° C using a 25 mL standard hydrometer, pH was measured with a pH meter (Denver Instrument model 15, USA), and chromaticity was measured with a colorimeter (CM-3500d, Minolta). Co., Ltd., Japan) measured L (brightness), a (redness), and b (yellowness) value 5 times, and represented it as the average value. The transmittance was measured at 690 nm using a spectrophotometer (Shimadzu UV-1601pc, Kyoto, Japan), and the content of dissolved tar was obtained by heating and drying in a dry oven at 105 ° C in 50 mL of evaporation plate. The basis weight is expressed as weight percentage of mortality. The total organic acid content was measured by diluting 1 mL of force 100 times and adding phenolphthalein and titrating with 0.1N NaOH solution according to the following formula.The total phenolic compound content was obtained by diluting the force of 100 times and taking 0.5 mL to obtain 10% Folin Reagent 2.5. ₂ mL of 7.5% Na ₂ CO ₃ was added thereto, and then the absorbance was measured at 750 nm after standing at room temperature for 30 minutes. The measurement result calculated the content using the calibration curve.

Total organic acid content (%) = 0.1 N NaOH titration × 0.1 N NaOH F × 0.006005 × 100 / sample volume (mL)

2. Component Analysis

end. General ingredient

According to the AOAC method (「Official methods of analysis, 14th ed., Association of official analytical chemists.」 Arlington, Virginia, 1984, pp.431.), Moisture is 105 ℃, atmospheric pressure drying and crude protein is micro-Kjeldahl. Crude fat was analyzed by soxhlet extraction and ash analysis. Carbohydrates were expressed by subtracting water, crude protein, crude fat and ash from 100. All measurements were expressed as the average of three repeated measurements. .

I. Glass sugar

Take 1 g of sample and 50 mL of 80% ethanol in a round Erlenmeyer flask, heat for 5 hours at 75 ° C (using heating mentle), filter with Whatman filter paper (No. 2), and filter the filtrate with a rotary vacuum evaporato. The mixture was concentrated under reduced pressure at 10 mL, and analyzed by HPLC (High Performance Liquid Chromatography) using 10 mL. The conditions are shown in Table 1.

All. amino acid

0.5 g of the sample and 3 mL of 6 N HCl were degassed in a digestion tube, and dehydrated at 121 ° C. for 24 hours. After filtration, the solution was concentrated under reduced pressure with a rotary vacuum evaporator, and the mixture was applied to sodium phosphate buffer (pH 7.0), 10 mL, 1 mL of the solution was filtered through a membrane filter (0.2 μm). The following amino acid dynamic analyzer was analyzed, and the conditions are shown in Table 2.

la. fatty acid

For fatty acid analysis, 5 g of the sample was homogenized by a heating blender according to the AOAC method, 10 mL chloroform and 20 mL methanol were added thereto, followed by homogenization for 2 minutes, and then 10 mL chloroform was added for 30 seconds. After filtration, the mixture was left for 30 minutes, the upper layer was removed, anhydrous sodium sulfate (Na ₂ SO ₄₎ was added thereto, followed by dehydration and concentration under reduced pressure with a rotary vacuum evaporator.

100 mg of fat was taken up, dissolved in 5 mL toluene, methylated with BF _3- MeOH reagent, and analyzed by Gas Chromatography (GC).

hemp. Vitamin A and Vitamin E

Take 0.5 g of sample, 0.1 g of ascorbic acid and 5 mL of ethanol, heat at 80 ° C for 10 minutes, add 0.25 mL 50% KOH solution, heat for 20 minutes, add 24 mL of distilled water and 5 mL hexane, and add 1,150 ×. Centrifuge for 20 minutes at g. After separating the supernatant, 40 mL of hexane was added and centrifuged to separate the supernatant, and then, distilled water was added and left for 10 minutes to remove the lower layer. After repeating this process three times, the solution was combined, dehydrated by adding anhydrous Na ₂ SO ₄ , concentrated under reduced pressure to 3 mL using a rotary vacuum evaporator, and analyzed for vitamin A and vitamin E contents by HPLC (High Performance Liquid Chromatography). Table 4 is as follows.

bar. Organic acid

Take 1 g of sample and 50 mL of distilled water in a Erlenmeyer flask, heat it for 4 hours in an 80 ° C water bath, filter with Whatman filter paper (No. 2), concentrate the filtrate under reduced pressure with a rotary vacuum evaporater, and dilute to 10 mL with distilled water. HPLC analysis was performed and the conditions are shown in Table 5.

four. Mineral

0.5 g of sample, 10 mL of 20% nitric acid and 3 mL of 60% HClO ₄ (Perchloric acid) were taken and heated to 50 mL with 0.5 M nitric acid until it became clear. The standard solution for each analysis item was mixed and 8 mL of each vial was used as a standard solution. 0.5 M nitric acid was used as a control, and the analysis conditions are shown in Table 6.

Ah. Positive and negative ions

5 g of the sample was taken in an evaporating dish, incubated for 24 hours, allowed to cool for 30 minutes, and 4 mL of a solution having HCl: distilled water = 0.5: 3.5 and 10 mL of distilled water were added to dissolve the ash while warming on a water bath. The solution was analyzed by HPLC with 100 mL of distilled water. The conditions are shown in Tables 7 and 8.

3. Determination of Antioxidant Activity of Death in vitro.

end. Antioxidant Activity Measured by Oxidation Stability Tester (Rancimat)

30 μl, 50 μl and 70 μl of mortality were taken and added to oil (soybean oil) and measured the induction time with Rancimat 676 (Metrohm, Swiss) to compare the antioxidant activity of each fraction extract. Antioxidant index (AI) was obtained by dividing the induction time of the experimental group to which each fraction was added.

Rancimat measurement conditions were 3.0 grams of the sample was taken in the reaction vessel and 70 mL of distilled water in the measuring vessel and the oxidation stability at 20 ℃ / air flow rate at 110 ℃ was compared. All measurements were averaged after 3 replicates.

4. Determination of HMG-CoA (3-hydroxy-3-methyl glutaryl CoA) reductase inhibitory activity in vitro .

end. Yeast culture

HMG-CoA reductase was used as a microsomal protein of Saccharomyces cerevisiae ATCC 42949 purchased from the Korean spawn association. Under anaerobic conditions, S. cerevisiae ATCC 42949 was pre-incubated at 30 ° C. for 24 hours in 1% glucose, 0.5% polypetone and 1% yeast extract medium, followed by 3% glucose, 0.5% polypeptone, 0.5% yeast extract, 0.5% K ₂ HPO ₄ , 0.5% KH ₂ PO ₄ Medium was inoculated with 1% of the pre-culture was incubated at 30 ℃ for 15 hours.

I. Preparation of Microsomal Protein

The culture solution was centrifuged at 4,000 rpm for 15 minutes, washed twice with distilled water (4 ° C), and the cells were collected to obtain 5-15% (w / v) in 0.1 M triethanolamine buffer (pH 7.4) containing 20 mM EDTA. Diluted. After homogenization at 10,000 psi for 5 minutes with cell homogenizer, centrifugation was performed at 8,000 rpm for 15 minutes to remove mitochondria, and supernatant was ultracentrifuged at 34,000 rpm for 90 minutes to obtain microsomes. The isolated microsomes were washed with the above buffer with 2 mM DTT, quantified protein (52) and prepared at 10 mg / mL.

All. HMG-CoA reductase Inhibitory Activity

Hulcher et al. (Hulcher, FH., Oleson, WH., "Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutary CoA recuctase by measurement of coenzyme A. J. Lipid Res 14 : 625-631, 1973.) The reaction solution was prepared as follows: reaction solution was prepared with yeast microsomal protein 1 mg, HMG-CoA 150 nmol, NADP ⁺ 2μmol, glucose-6-phosphate 3μmol, glucose-6-phosphate dehydrogenase 2 units, and killed 30 μL, 50 μL and 70 μL were added to a final volume of 1 mL, after 30 min reaction at 37 ° C. 20 μL of 10 mM sodium arsenite solution was added and after 1 min 2M citrate buffer containing pH 3% sodium tungstate (pH 3. 5) After adding 0.1 mL, the reaction was stopped for 10 minutes at 37 ° C. After centrifugation at 15,000 rpm for 5 minutes, the precipitated protein was removed, 1 ml of the supernatant was taken, and 0.2 mL of 2 M Tris buffer (pH 10.6) was added. The pH of the reaction was adjusted to 8.0 by adding 0.1 mL of 2 M Tris buffer (pH 8.0) 0.4 M sodi 50 μL of um arsenite was added and reacted for 5 minutes to form a dithiolarsenite complex, 1 mL of the reaction solution was added, 20 μL of 3 mM DTNB was added, and the absorbance was measured at 412 nm. After the determination, the amount of CoA-SH produced was determined by the following equation.

CoA-SH production amount {(rm nmol = 1.43 × A (reaction) -A (control) / 0.136 × time)}

HMG-CoA reductase inhibition rate = CoA-SH production amount (test-control) / control × 100

Where 1.43 is the dilution factor of the reaction solution,

         0.136: extinction coefficient of CoA-SH,

         control: control (without death),

         reaction: Experiment zone (additional force)

5. In vivo Hyperlipidemia Inhibitory Effect Experiment

end. Breeding and Diet of Laboratory Animals

Sprague-Dawley male rats were supplied from Chosun University Experimental Animal Center for 1 week with solid compound feed (Samyang feed) and water, and then weighed 100 ± 10 g by randomized complete block design. Each group was selected from the group of 10 (NOR), high cholesterol diet (CON), low dose (5BL), low dose and high cholesterol diet (5BCB), high dose and high cholesterol diet. The animals were divided into five groups (10BCB) for six weeks.

Hypercholesterolemia-induced diet was prepared by adding 1% cholesterol and 0.25% sodium cholate based on AIN-93. Water and diet were supplied without restriction, the room temperature was maintained at 18 ± 2 ^o C, and the lighting was controlled at 12 hour intervals (08:00 to 20:00). While observing the condition of the animals during the experiment period, the body weight was measured at weekly intervals, and the dietary intake was measured at two-day intervals. The weight gain of the breeding period was divided by the dietary intake of the same period. ) Was obtained.

I. Experiment animal kill

Rats fasted for 18 hours before treatment were anesthetized with ether, collected from the abdominal aorta, left at room temperature for 20 minutes, and centrifuged at 1,150 × g for 10 minutes to separate serum. This serum is described in Reitman and Frankel (Reitman, S. and Framkel, S., "A colorimetric method for the determination of serum glutamic oxaloacetic determination and glutamic pyruvic transaminase." Am. J. Clin. Pathol., 28: 56, 1957. The kit prepared in) was used to measure alanine aminotransferase (ALT) and asparate aminotransferase (AST) activity. The liver was extracted, washed with ice cold saline (0.9%), and then drained. The weight was measured and stored at −70 ° C. and used for enzyme measurement.

All. Serum Lipid Level Measurement

Total cholesterol content in serum was determined by Richmond's enzyme method (Kim JI., Choi JH .. "Effect of brown algae component on obese rats induced by a high fat diet I. Body weight, feed and gross efficiences, body fat content and obesity index. Kit (Am202-k, Asan) prepared in accordance with Kor. J. Gerontol. 3: 33-38, 1992.), high-density lipoprotein (HDL) cholesterol content was measured by enzyme method (Lee KS., Seo JS., Choi TS., "Effect of sea tangle and hypoglycemic agent on lipid metabolism in diabetic rats." J. Korean Soc. Food Sci. Nut., 27: 960-967, 1998). -k, Asan) and triglyceride content were prepared according to McGowan et al. (Yagi K. "Lipid peroxides and human disease." Chemistry and physics of lipid. 45 : 337-342, 1987.). Asan). Low density lipoprotein (LDL) cholesterol content in total cholesterol-HDL cholesterol- (triglyceride / 5) (Friedwald, WT, RL Levy and DS Fredrickson. "Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. " Clin. Chem. 18 : 499-502, 1972.) and cholesteryl ester contents were calculated by dividing total cholesterol by free cholesterol. The atherosclerotic index (AI) used to determine the risk of cardiovascular disease was calculated by dividing the total cholesterol of total cholesterol-HDL cholesterol / HDL cholesterol and HDL cholesterol by the total cholesterol.

la. Soy lipid concentration measurement

Lipids in the liver are described in Folch (Folch, J., M. Lees and GH Sloane-Stanly. "A Sample method for the isolation and purification of total lipid from animal tissue." J. Biol. Chem. 226: 497-509, According to 1957.), 0.9% NaCl was added to the liver tissue, homogenized with a homogenizer, and a predetermined amount was taken, followed by filtering with CHCl ₃ -MeOH (2: 1, v / v). CaCl ₂ was added to the filtrate, mixed and centrifuged at 2000 rpm for 10 minutes to remove the supernatant, followed by drying over N ₂ gas. The dried sample was dissolved in CHCl ₃ , treated with triton X-100, and the total cholesterol and triglyceride contents were measured in the same manner as the serum.

As described above, the killing force produced according to the present invention confirmed the effect of improving hypercholesterolemia. Since the acne-causing bacteria are fat-affinity microorganisms, the oily skin is further exacerbated by the hyperplasia of sebum, thereby inhibiting the action of the killing bacteria against acne-causing bacteria. The purpose of this study is to examine the correlation between hyperlipidemia and acne with reference to find out whether acne can be improved by death.

Example 2

1. Acne improvement effect experiment

end. In vitro Growth Inhibition Against Acne Strains

Antimicrobial activity and viable cell counts of the strains of Propionibacterium acnes , Staphylococcus epidermidis and Malasseizia furfur were measured.

① Use strain and medium

Propionibacterium acnes KCTC2358 was distributed from the Korea Institute of Biotechnology and Biotechnology Research Institute Gene Bank, Staphylococcus epidermidis ATCC1228 from National Institutes of Health, and Malasseizia furfur KCCM 12679. Media and culture conditions for each strain are shown in Table 10. After P. acnes was activated at -20 ° C for 3 days before the experiment, M. furfur and S. epidermidis were inoculated in slant made of TM agar and Tryptic soy agar, incubated at 37 ° C for 24 hours, and then stored at 4 ° C. Used.

② Antibacterial activity search

The search for antimicrobial activity against acne-causing strains of mortality was performed by the paper disc method (Kuk, JH, SJ Ma, and KH Park. "Isolation and characterization of cinnamic acid with antimicobial activity from needle of Pinus densiflora." Korean J. Food Sci. Technol 29: 823-826, 1997.). After absorbing the killing capacity (30 ℃ L, 50 ℃ L and 70 ℃ L (v / v)) by paper disc (℃ 8mm, Whatman), each strain (approximately 3.43 × 10 ⁶ cfu / mL) was placed on the surface of the medium. P. acnes was anaerobicly cultured at 37 ° C for 5 days, and M. furfur and S. epidermidis were cultured at 37 ° C for 48 hours.

③ Measurement of viable cell count

Was added to each dose tabasheer to the growth medium of each strain after each strain (approximately 3.43 × 10 ⁶ cfu / mL) was inoculated P. acnes is an anaerobic culture at 37 ℃ 4 ilgan, M. furfur for 24 hours with shaking at 37 ℃ Cultures, S. epidermidis was incubated at 37 ℃ for 24 hours, sampled by time period and diluted with 0.85% saline, and the number of viable cells was measured.

I. In vivo Acne improvement

① Management target

The patients were diagnosed with acne from May-December 2003 by acne-prone rash and purulent skin.

② Management method

Visitor's gender, age, distribution of skin rashes, Dr. Don Donald Phillsbury's taxonomy (Hyun-Joo Kim, Na-Young Lee, Sun-Joo Ko, Hye-Jung Lee, Hyun-Hwa Lee, Kyung-Im Choi. 『Eesthetic Salon Treatment.』 2000, pp.122-125 .) Investigate acne grade and skin condition (Table 11), add 10 mL of distilled water to 3 mL of mortality, moisten it on sterilized cotton wool, attach it to the face for 20 minutes, and then remove it continuously. Records were maintained and observed for ˜5 months.

2. Statistical Processing

The results obtained in this experiment were calculated using the SPSS statistical package to calculate the mean and standard error per experimental group. After one-way analysis of variance, Tukey (T) -test was performed at p <0.05. Significance between the mean of each experimental group was tested.

(Experiment Results and Discussion)

1. Physical and chemical properties

The physical and chemical properties of crude A and refined B are shown in Tables 12 and 13. Specific gravity was not different between death A and death B. Transparency showed 0.163 of crude death A, but decreased to 0.049 during the refining process.The tar component contained 0.814% of crude death A, but 0.177% during refining. This was reduced by more than 78%.

In colorimeter analysis, the contrast became clear, with a crude color of 53.78 representing 92.41 during purification and the redness lowered from 29.12 to 3.02, eliminating most of the initial reddish light. In this result, the greater the tar content, the lower the L * (brightness). From this, it was found that the tar content and brightness were inversely correlated, and it was assumed that tar was the main substance affecting the color of mortality.

In chemical properties, pH was 3.54 in B.B., changed to 2.46 after purification to increase acidity, total organic acid decreased by about 63% from 3.68% to 1.35%, and total phenolic content was 57.79 mg / mL at 295.43 mg / mL. About 80%. Bamboo contains macromolecules such as cellulose, hemicellulose and lignin. Hemicellulose is first thermally decomposed around 180 ° C, followed by cellulose at 240 ° C and lignin at 280 ° C. , Lee Yun-hwan, "Composition of Commercial Wood Vinegars." J. Korean Soc. Agric. Chem. Biotechol. 44 (4) : 262-268, 2001.).

Organic acids and neutral components are mainly produced from hemicellulose and cellulose, and phenolic compounds are mainly produced by lignin decomposition (Park Sang-bum, Kwon Su-deok, Kim England, Gu Jaun "Bamboo Credit Rating." , 66 : 97-105, 2002.).

Therefore, in this experiment, the total content of phenolic compounds is different (Eung Jong-bang. "Establishment of modern manufacturing system of bamboo power and purification and characterization of bamboo vinegar." 『Intermediate report of Damyang-gun bamboo bio-industry research support project』, 2003 The reason for this difference is that the temperature at which lignin decomposes during the process was not sufficiently maintained.

In this study, it is considered that color and other substances with tar are removed during the refining process, and transparency and whiteness can be secured, and acidity is increased by decreasing pH. Is considered to be increased due to the removal of a large amount of high boiling point substances such as organic acids and phenol derivatives.

2. Component Analysis

end. General ingredient

The crude constituents of crude (A) and refined (B) are shown in Table 14. Crude protein content of A is 0.35%, B is 0.21%, crude fat content is A1 0.61% and B is 0.34%. And no other components were detected.

I. Glass sugar

The free sugar content of the kill force is shown in Table 15. Total free sugars decreased by 120.3 mg% for A and 32.4 mg% for B, reducing about 73% of the free sugar during the refining process. In free sugars, lactose content was the highest in two species, followed by fructose and glucose, followed by ribose and maltose in A and arabinose, galactose, ribose and maltose in B.

All. Amino acid composition

The amino acids contained in the killings were analyzed and could not be detected.

 la. Fatty acid composition

The results of analyzing the fatty acids contained in the killing force are shown in Table 16.

The total fatty acid content was 139.1 mg% in A and 99.9 mg% in B, which decreased about 25% of fatty acids during the refining process. Palmitoleic acid contents in A and B were 80.8% and 66.8%, respectively, followed by linoleic acid. .

hemp. Vitamin A and Vitamin E

The vitamin A and vitamin E contained in the killings were not detected.

F. Organic Acid

The organic acid content in the bamboo power is shown in Table 17. The total organic acid content was 2.65 mg% in A and 0.33 mg% in B, which decreased about 88% of organic acids during the refining process. This result is described by Hwang Bh, Koo JO, Kim YS, Nun SP, Moon CK, Park KH, Ahn WY, Lee JY, Lee HJ, Cho NS.Wood Biomass, Sinjinmunhuasa, Seoul, 1998, 31-87 Among the three major components of this tree, cellulose, hemicellulose, and lignin, cellulose pyrolyzes above 300 ° C, forming charcoal, acetic acid, formic acid, and small amounts of furfural derivative gas products, and hemicellouse is most sensitive to heat. It was consistent with the report that it was decomposed in the temperature range of 260 ℃ to produce furfural derivatives with acetic acid, formic acid.

four. Mineral

Table 18 shows the results of analyzing the mineral content in the bamboo.

The total mineral content was 81.5 mg% for A and 35.0 mg% for B, which decreased about 57% of the minerals during purification. By mineral, A had the highest Ca content of 37.7 mg%, followed by K, Na, and Fe, and B had the highest Ca content of 17.7 mg%, followed by Fe, K, and Na. The highest reduction rate of K was found during the purification process. This result is described by Fan-Zhu Lee and Jong-Bang Eun .. "Physicochemical characteristics of Bamboo Smoke distillates processed by mechanical steel kiln and traditional earth kilm." J. Korean Soc.Food Sci.Nutr ., 31 (2): 251-256, 2002.) were consistent with the reports that Ca, Fe, and K were found in the minerals contained in bamboo vinegar.

Ah. Positive and negative ions

The results of the analysis of the cations and anions in the killing force are shown in Tables 19 and 20. In the case of the killing agent, K ⁺ was 3.07 mg% of the cations, followed by Ca and Na, and the killing force B In the case of, many cations were reduced during the refining process, with the highest Na content, followed by K and Ca. For the anion Cl ^-, SO ₄ ^-2, an Many.

3. Antioxidant Activity Measured by Rancimat in vitro

30 μL, 50 μL, and 70 μL of purified mortality were added to the soybean oil, and the antioxidant power was measured using Rancimat. Antioxidant index (AI) of 30μL was the same as the control, 50μL 1.02 and 70μL was 1.09 showed higher antioxidant activity than the control. Lee et al. (11) reported that the crude mortality contained about 300 kinds of organic materials, and among them, alcohol, aldehydes, and lignin were found to exhibit antioxidant activity due to the large number of phenolic compounds formed by pyrolysis. It is believed that the result is different from the results of this and the like due to the reduction of a large amount of total organic acids and phenolic compounds during the purification process.

4. HMG-Co A reductase Inhibitory Activity in vitro

Table 22 shows the results of measuring the inhibitory activity against HMG-CoA reductase by varying the capacity of tablets. In this experiment, 70μL of the tablets showed 57.9% of inhibitory activity against HMG-CoA reductase, followed by dose-dependent inhibitory activity of 50μL of 36.0% and 30μL of 7.6%. The results of this experiment showed that Yi-Haung Lee, "Study on the screening and application of 3-hydroxy-3-methylglutaryl CoA reductase inhibitor from pinus strobus extracts" PhD Thesis Kang-won University, 1994. The results were similar to those reported that buckwheat husks showed high inhibitory activity of 65.5% against HMG-CoA reductase. Development is expected to reduce the material. HMG-CoA reductase is a regulatory enzyme of cholesterol synthesis in the body, and is present in the transgenic networks of the liver, small intestine, adrenal glands, and gonads. The activity is also regulated by the amount of cholesterol in the body, 26-hydroxycholesterol and phosphorylation. In particular, the phosphorylation reaction deactivates the enzyme and activates it when dephosphorylation occurs (Peter A. Edwards and R, Gordom Gould ..? Turnover rate of hepatoc 3-hydroxt-3-methylglutaryl coenzyme A reductase ad determined by use of cycloheximide . " J. of Biological Chm ,. 947 (5) : 1520-1524, 1972.). Increasing intracellular free cholesterol inhibits the transcription of the HMG-CoA reductase gene, which reduces LDL-receptor counts and cholesterol acyl transferase ( ACAT) activity is promoted, so competitive inhibitors of HMG-CoA reductase not only inhibit cholesterol synthesis, but also increase the number of LDL-receptors, thus lowering the amount of LDL cholesterol in serum. Drug development is largely focused on the search for HMG-CoA reductase inhibitors (Brown, MS and Goldstein, JL "A receptor-mediated pathyway for cholesterol homeostasis." Science , 232 : 34-47, 1986.).

5. Inhibition of hyperlipidemia in vivo

A. Weight gain and dietary efficiency

The weight gain rate of rats bred after 6 weeks of death was shown in Figure 2, the liver / weight ratio and dietary efficiency is the same as Figures 3 and 4. From 4 weeks, the control group (CON fed only high-cholesterol diet: CON) had a weight gain rate of 3.52 ± 0.07, which was higher than that of the normal group (NOR fed only base diet: NOR), but combined with low mortality and high dose. From 6 weeks, it was 3.77 ± 0.04 and 3.75 ± 0.09, respectively, which was significantly slower than CON 4.11 ± 0.04. The dietary efficiency of CON increased 0.172 ± 0.01 at 6 weeks compared with NOR 0.141 ± 0.01. However, the dietary efficiency was decreased by low dose and high dose combined dose but no significant difference. The hepatic / weight ratio did not show any significant change among the experimental groups, but CON increased to 1.72 ± 2.06, NOR 1.41 ± 1.09, and the low-dose low-dose group (5BL) 1.38 ± 1.07. Phosphorus of some vegetable seed oil feeds on body lipid composition in rats. Ph. D. dissertatio, Kyunsang University , pp. 81, 1990. It is thought to have accumulated and increased to triglycerides.

I. Total Cholesterol, Neutral and Phospholipid Concentrations in Serum

The total cholesterol, triglyceride, and phospholipid content in the serum of rats measured after 6 weeks of treatment with different levels of high cholesterol diet and mortality (5%, 10%) are shown in Figs.

The total cholesterol concentration of CON fed the high cholesterol diet was 178.21 ± 29.38 mg / dL, which was significantly increased compared to NOR 123.22 ± 11.59 mg / dL, and the low-dose low dose group (5BCB) was 143.19 ± 21.15 mg / dL. There was no significant reduction compared to that of the high dose group (10BCB), but the total cholesterol concentration was reduced by about 30% to 125.18 ± 22.22 mg / dL. Hypercholesterolemia is an indicator of arteriosclerosis, which is due to the synthesis of triglycerides and secretion of chylomicron in the small intestine, the synthesis of triglycerides in the liver, the synthesis and secretion of VLDL and LDL-cholesterol, the decrease of HDL-cholesterol synthesis and the decrease of lipase activity. It has been known to be reduced due to the removal of triglycerides from peripheral tissues (Miller, n. E. "The evidence for the antoatherogenicity of high density lipoprotein in man." Lipid , 13: 914-919, 1987., Kim, SY, Kim, HS, Kim, SH, Kim, HS, Su, IS and Chung, SY "Effects of the feeding Platycodon grandiflorum and Codonopsis lanceolata on the fatty acid composition of serum and liver in rats." J. Korean Soc. Food Nutr., 22 : 524-530, 2003.).

In the present invention, the total cholesterol level of the high cholesterol diet fed group was significantly higher than that of the normal group (Jang, JY, M, K, Ldd, MJ Kim and SY Cho. "Effect of fiber on serum lipid metabolism in rats with diet-induced cholesterolemia. " J, Korean Soc. Food Sci. Nutr . 27 : 1211-1216. 1998.), presumably due to the accumulation of free cholesterol and cholesteryl esters in the liver by dietary cholesterol It is also considered that the serum total cholesterol concentration is about 30% lower than that of the high cholesterol diet group in the 10% killing group.

The concentration of triglyceride was 83.37 ± 8.17 mg / dL of CON, which was much higher than NOR 54.76 ± 2.55 mg / dL. However, the combined dose of 5BCB was 62.03 ± 5.93 mg / dL and 10BCB was 50.84 ± 3.07 mg / dL. Compared with CON, it decreased by 26% and 39%, respectively. In particular, 10BCB was lower than NOR. Yang, J, L., J. Suh and YS Song. "Effects of dietary fibers on cholesterol metabolism in cholesterol-fed rats." J. Korean. Soc. Food Nutr. 25 : 392-398, 1996. As reported by), the effect of pulmonary administration was to activate the lipoprotein lipase on the capillary wall, thereby promoting the degradation of the major microcarriers of neutral lipids, Kilomicron and VLDL-cholesterol.

Fatty liver due to high fat diet was mainly due to the decrease in phospholipid synthesis (Eun-Jung Chung, Soo-Yeon Kim, Ji-Young Kim, Ji-Young Ahn, Jung-Wha park, Myung-Wha cha, Tang-Cha) Lee., "Effects of soy protein concentrate and age on plasma lipids and phospholipid fatty acid patterns in female rats." J. Korean Soc. Food Sci. Nutr . 32 (2) : 269-277, 2003.) The concentration of phospholipids was 347.58 ± 17.70 mg / dL, which was significantly lower than 424.47 ± 45.45 mg / dL of NOR in the high cholesterol diet and 380.00 ± 22.31 mg / dL and 391.54 ± 23.17 mg / dL, although not significant, increased compared to CON. Increased phospholipid concentrations in this study suggest that death can prevent and prevent the progression of alcoholic fatty liver (Oda, T., Shikate, T., Natio, C., Suzuki, H. and Kametaka, T). . "Phospholipid fatty liver: a report of three cases with a nw type of fatty liver." Jpn. J. Exp. Med , 40 : 127-140, 1970./ Sinclair, AJ an Collins, F, D. "Fatty livers in rats deficient in essential fatty acids. " Biochem. Biophys. Acta . 152 : 498-510, 1968).

All. LDL-cholesterol concentration, HDL-cholesterol concentration, HDL-cholesterol / total cholesterol ratio and arteriosclerosis index in serum

The results of the effects of LDL- and HDL-cholesterol concentration, HDL-cholesterol / total cholesterol ratio and arteriosclerosis index on the death and high cholesterol diet in rats for 6 weeks were shown in FIGS. 8 to 11.

LDL-cholesterol concentrations were elevated to 123.64 ± 11.94 mg / dL, 41% higher than NOR's 87.93 ± 16.37 mg / dL on a high-cholesterol diet. / dL decreased 12% and 20%, respectively, compared to CON, and 10BCB was close to the normal group.

In general, LDL-cholesterol is a major carrier of cholesterol in the blood. Accumulation of cholesterol in arterial vessel walls promotes arterial stiffness and promotes thrombogenesis by complex actions such as vascular endothelial cell injury and blood viscosity. It is reported that there is a close correlation between cholesterol concentrations and the occurrence of coronary heart disease (Kannel, WB, WP Castelli and T. Gordon. "Cholesterol in the prediction of atherosclerotic disease." Am. Intern. Med. 90 : 85- 91, 1979.).

The HDL-cholesterol concentration was reduced by 19.89 ± 5.88 mg / dL by 30% compared to NOR 28.63 ± 4.80 mg / dL by feeding high cholesterol diet, and the HDL-cholesterol / total cholesterol ratio was reduced by about 48%. The hardening index increased by about 142%. The mortality was elevated to 24.34 ± 3.11 mg / dL and 26.38 ± 3.66 mg / dL, respectively, and the HDL-cholesterol / total cholesterol ratio was higher than CON, and the atherosclerosis index was 45% and 53%, respectively.

Since HDL cholesterol carries cholesterol of the peripheral tissues to the liver and synthesizes bile acids from cholesterol and excretes it into the intestine, HDL cholesterol has a protective effect against atherosclerosis. Therefore, the ratio of HDL-cholesterol to total cholesterol concentration or LDL-cholesterol to HDL-cholesterol concentration rather than serum total cholesterol concentration is recognized as a good indicator for predicting the development of cardiovascular disease.

As a result of the above experiments, death was administered to reduce LDL-cholesterol concentration and camellia cure index, and to increase HDL-cholesterol concentration and HDL-cholesterol concentration to total cholesterol, which may help prevent and treat arteriosclerosis. I think.

la. Free Cholesterol and Cholesteryl Ester Concentrations in Serum

The ratios of free cholesterol, cholesteryl ester concentration and cholesteryl ester in the serum of rats administered with high cholesterol and death concentrations are shown in FIGS. 12 and 13.

The concentration of free cholesterol in CON fed high-cholesterol diet increased to 50.89 ± 2.79 mg / dL compared to NOR's 31.83 ± 3.70 mg / dL, and 5BCB was reduced to 40.68 ± 3.48 mg / dL, but not a significant effect. 10BCB decreased by 26% to 37.47 ± 5.05 mg / dL, indicating a significant change. On the other hand, the cholesteryl ester concentration increased CON by 136.71 ± 25.60 mg / dL, more than about 96% of NOR compared with 69.78 ± 16.58 mg / dL, and the low dose group showed no significant decrease, but the high dose group showed 92.97 ±. Reduction of approximately 32% to 39.93 mg / dL, and associated with increased phospholipid concentrations in the table, it is expected that death administration will be effective in improving and preventing hypercholesterolemia.

Most of the cholesterol in the blood is absorbed by the small intestine, and 80% of it is combined with fatty acids of lipoproteins. In humans, the ratio of cholesteryl esters to total cholesterol is about 70% normal, and the decrease in cholesteryl esters is an important indicator in diagnosing liver disease and has been reported to be elevated when hypercholesterolemia is present (Normura). M, Nakajima Y, Abe H. "Efects of long administration indigestable dextrin as soluble dietary fiber on lipid and glucose metabolism." J. JAN Food Nutr . 45 (1) : 21-25, 1992.).

hemp. AST, ALT and ALP activity in serum

The effects on the AST and ALT activity in the serum of rats fed the high cholesterol diet for 6 weeks are shown in Figure 14-16. In the case of AST, CON fed only the cholesterol-containing diet was 175.67 ± 13.63 units, which was significantly different from 150.17 ± 9.59 of the NOR fed only the normal diet, and 5BCB was 173.21 ± 9.45 units and 10BCB was 163.46. It dropped to ± 7.07 units. In the case of ALT, CON was 95.99 ± 5.45 units, which was significantly increased compared with 74.11 ± 2.76 units of NOR. The ALP activity CON was 38.41 ± 2.02 units, which was increased compared to 32.37 ± 2.49 units of NOR, but it was not a significant change.

Aminotranferase (ALT, AST) is a representative inflammatory enzyme that indicates the degree of tissue damage. It is nonspecific and its activity is relatively correlated with the degree of cytotoxicity and is more sensitive than other blood efflux enzymes. Therefore, when toxic cells are caused by hepatitis, fatty liver, cirrhosis and liver cancer, hepatocytes are destroyed, and enzymes in the cells are leaked into the blood to increase serum enzyme activity. Intracellular activity is greater than serum activity. ALP is also an enzyme present in the tissues of the body and is used to observe the diagnosis and prognosis of hepatitis, cirrhosis, etc. It is mainly produced by the liver due to bile duct excretion and bile duct hypertension. In this experiment, AST, ALT, and ALP activity increased by high cholesterol diet was reduced by the administration of death. It is expected to be used for the prevention and treatment of death of hepatocellular damage.

bar. Soy total cholesterol and triglyceride concentration

The contents of total cholesterol and triglycerides in the liver of rats administered by varying the high cholesterol diet and death concentration are shown in FIGS. 17 and 18.

CON was 16.31 ± 0.24 mg / dL, which was significantly increased compared to NOR's 12.08 ± 0.32 in the liver, and the administration of force did not reduce the increased total cholesterol level at low doses, but in the high dose group, 10BCB was 13.87. Significantly lowered to ± 0.03 mg / dL. The triglyceride level was 22.45 ± 2.34 mg / dL, which was higher than that of NOR 16.89 ± 1.05. Cholesterol becomes bile acid in the liver and is secreted into the small intestine.The bile acid used in this way is combined with cholesterol and lipids taken from the diet, reabsorbed, and then returned to the liver for use in the entero-hepatic circulation process. It regulates the amount of cholesterol in the body. In the present experiment, the total cholesterol level in the liver was significantly lower than that of the control group by the treatment of death (Sang-Mo Kang, Ji-young Shin, Sung-Joo Hwang, Seonggil Hong, Hye-Eun Jang and Mi-Hyun Park. , "Efects of Saengshik supplementation on health improvement in diet-induced hyper cholesterolemic rats." As reported by J. Korean. Soc. Food Sci. Nutr ., 32 (6) : 906-912, 2003.) Inhibition of resorption leads to a decrease in the amount of endogenous cholesterol, which may help to improve hypercholesterolemia.

6. Acne improvement effect

end. In vitro acne Inhibition of growth of causative bacteria

① antimicrobial activity

Growth inhibition of M. furfur KCCM 12679 , S. epidermidis ATCC 1228 , P. acnes KCTC 2358 , which are the acne-causing bacteria, are measured in Table 23 and FIG. 19.

70 μL of mortality showed the strongest effect on P. acnes , and similar inhibitory activities were observed for M. furfur and S. epidermidis at concentrations of 30 μL, 50 μL and 70 μL. In addition, as shown in the growth inhibition ring, the clear borderline of clearing ring was clearly shown, confirming the inhibitory effect of the skin-inflammatory bacteria.

② growth inhibition

As shown in FIG. 20, growth inhibition of S. epidermidis of S. epidermidis was slightly suppressed at the initial stage of growth of 30 μL, but increased as time passed. In the case of 50 μL and 70 μL, viable cell count began to decrease after 6 hours. After the time was reduced to 50 μL (2.53 × 10 ³ CFU / mL) and 70 μL (1.62 × 10 ² CFU / mL), the growth inhibition of the bacteria was obvious, and the growth inhibitory effect was obvious as the added dose increased.

Inhibition of growth of M. furfur of death was not observed in the 30 μL, 50 μL, 70 μL addition group as shown in FIG.

As shown in FIG. 22, the growth inhibition of P. acnes was inhibited after 30 days in 30 μL, but little change thereafter. 50 μL and 70 μL were all killed after 1 day, indicating high antimicrobial activity. It was. In this experiment, P. acnes showed the strongest inhibitory effect of P. acnes , followed by S. epidermidis and M. furfur .

I. In vivo Acne improvement effect

① Acne Ratings and Aspects

This management was designed for customers with acne rashes and purulent acne on the face and trunk. Acne grades based on Dr. Donald Phillsbury's classification method were classified as 1, 2, 3 and 4 It was one.

The appearance of the rash was not determined in any particular form, but the pustules, papules, and cotton cloth were mixed.

② management effect

The management results showed that the skin acne rash and purulent condition were improved as shown in skin care cases 1 (Table 24), 2 (Table 25), 3 (Table 26), 4 (Table 27) and 5 (Table 28) and FIG. Could.

The present invention as described above is first chemical composition in order to identify the bioactive efficacy of the tabasheer manufactured in conventional manner and nutrient analysis, physical and antioxidant activity in chemical properties, in vitro, HMG-CoA reductase inhibitory activity in in vitro, After confirming the effect of improving hypercholesterolemia in vivo , acne-causing bacteria are fat-affinity microorganisms, and in case of oily skin, acne worsens due to sebum hyperplasia. The following results were obtained as a correlation to examine that death improves acne rash and purulent condition.

1 . Purification process resulted in the removal of color-related substances such as tar, resulting in a transparent tablet power. The pH was increased from 3.54 of crude blood pressure to 2.46 after purification, total organic acid was about 63%, and total phenolic content was about. 80% was reduced.

2 . Free sugar is lactose, fatty acids palmotoleic acid and an organic acid is formic acid were the most common minerals Ca, K ⁺ cation is the most common anions are Cl ^-, SO ₄ ^-2, was a large amount detection.

3 . In vitro , antioxidant activity was measured by Rancimat, and 50 μL and 70 μL of mortality were higher than those of the control.

4 . In vitro , as a result of measuring HMG-Co A reductase inhibitory activity, 70 μL of the killing activity showed 57.9% and 36.0% of the inhibitory activity against HMG-CoA reductase.

5 . In vivo , the hypercholesterolemia-improving effect showed that body weight gain was significantly lower than that of the control group at 6 weeks, but the dietary efficiency was not decreased, but was decreased. The liver / weight ratio did not show a significant change between the experimental groups.

The total cholesterol level did not show a significant decrease in the low-dose group compared to the control group, but the high-dose group decreased the total cholesterol concentration by about 30% compared to the control group.

Neutral fat mass was significantly reduced by 26% and 39%, respectively, compared to the control group.

The cognitive mass was lower than that of the normal group by the high-cholesterol diet, but was not significantly increased compared to the control group by the administration of thoracic diet.

The LDL-cholesterol concentration was 41% higher than that of the normal group by the high-cholesterol diet, but significantly decreased by 12% and 20%, respectively, compared to the control group.

The HDL-cholesterol concentration was reduced by 30% compared to the normal group by the high-cholesterol diet, and increased compared to the control group by the combination of death, the HDL-cholesterol / total cholesterol ratio was reduced by about 48%, and the arteriosclerosis index was about 142. % Increased.

The concentration of free cholesterol increased by dietary high cholesterol diet was decreased by low dose mortality, but it was not significant, but 26% was decreased by high dose.

On the other hand, cholesteryl ester concentration increased more than about 96% compared to the normal group by the high cholesterol diet, and the high-dose group decreased by 32% compared to the control group.

The total cholesterol in the liver was significantly increased in the high cholesterol diet compared to the normal group, and the administration of death did not reduce the increased total cholesterol level at low doses, but the high dose group significantly decreased the amount of triglycerides. There was no change.

ALT activity was significantly increased in the high cholesterol diet compared to the normal group, but was significantly decreased by the administration of the mortality. However, ALT and ALP activity did not show a significant difference by the force administration.

6 . The antimicrobial activity of P. acne was strong against P. acnes among the acne bacteria, followed by S. epidermidis and M. furfur , and the treatment of purulent acne with death diluent improved rash and purulent condition.

As described above, the killing produced in accordance with the present invention inhibited the activity of HMG-Co A reductase in i n vitro and compared to the group fed high cholesterol diet, total cholesterol, LDL-cholesterol concentration and neutral lipid concentration, etc. In addition, HDL-cholesterol, phospholipid concentrations, etc. are believed to be effective in the prevention and treatment of fatty liver and arteriosclerosis. It is expected to be used for the management of purulent acne, which is exacerbated by sebaceous hyperplasia of oily skin by worsening and inhibiting growth.

Claims (3)

In order to extract acne killing water, Cutting a 3-4 year-old cotton rod ( Phyllostachys nigra var. Henonis) about 20-30 cm, standing in a spring for 12 hours, and then drying it in the shade to remove the nodes at both ends, followed by vertical division ; 25kg of ingredients are put in a charcoal kiln and the amount of air is controlled and heated to 900 ~ 1000 ℃, and the collected juice is cooled to 80 ~ 150 ℃ by passing cooling water around the communication to recover 1.5L (SG 1.07) liquid. After the second step (recovery rate: 5.6%) in the crucible to mature; Purified by adding 140 mg of activated carbon (200-250 mesh, Yakuri pure chemical Inc. Japan) to the aged juice solution, and distilled at 108 ° C. using a self-made atmospheric distillation apparatus to obtain 1.2 L (SG 1.00). Extracting purified bamboo force (B); Distilled water is added to 3mL of the third stage of the process for producing acne-improved death water, characterized in that to prepare 10 mL of the dead water. delete delete

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