KR100655593B1 - Composition for Liver Protection and Reduction of Serum Ethanol Level - Google Patents

Abstract

The present invention relates to a composition effective for the protection of the liver and the degradation of blood alcohol, the main ingredient of the three components from the barberry extract, brown root extract, goji berry extract, alderwood extract, nectar extract or milk thistle extract and turnip concentrate, green bean-rice bran fermentation It is a functional composition containing five active ingredients containing the extract as an auxiliary ingredient.

The composition of the present invention has a synergistic effect than when each component is individually used by mixing and using the respective components in an appropriate blending ratio, and protects or damages the liver damage caused by excessive drinking or chronic alcohol intake. The effect of healing the liver is very excellent, and the function of promoting alcohol metabolism and lowering blood alcohol after drinking is very excellent. When the composition of the present invention is eaten and consumed in the form of food, It protects liver damage and can lower blood alcohol levels.

Soy Sauce, Protection, Alcohol, Decomposition, Extract, Prickly Pear, Root, Wolfberry, Alder, Lavender, Milk Thistle, Green Bean, Rice Bran, Turnip

Description

Composition for Liver Protection and Reduction of Serum Ethanol Level             

1 is a graph showing the relative GOT, GPT lowering effect of the present invention

Figure 2 is a graph showing the change in blood alcohol concentration by the composition of the present invention

Figure 3 is a graph showing the effect of protecting the liver damage by excessive alcohol intake of the composition of the present invention

Figure 4 is a graph showing the protective effect of liver damage caused by chronic alcohol intake of the composition of the present invention

The present invention relates to a composition effective for the protection of the liver and the degradation of blood alcohol, the main ingredient of the three components from the barberry extract, brown root extract, goji berry extract, alderwood extract, nectar extract or milk thistle extract and turnip concentrate, green bean-rice bran fermentation It is a functional composition containing five active ingredients containing the extract as an auxiliary ingredient.

The composition of the present invention has a synergistic effect than when each component is individually used by mixing and using the respective components in an appropriate blending ratio, and protects or damages the liver damage caused by excessive drinking or chronic alcohol intake. The effect of healing the liver is very good, and also the function of promoting alcohol metabolism after drinking and lowering the blood alcohol is very good. When the composition of the present invention is eaten or consumed in the form of food, It protects liver damage and can lower blood alcohol levels.

The liver is the largest organ in the human body, and the main function of the liver is to process various substances introduced or produced in the body and to synthesize and supply important substances.

The liver stores the absorbed monosaccharides and glucose in the form of glycogen and serves as a nutrient storehouse where it is decomposed and released again when necessary, while maintaining a constant blood glucose level at all times.

There are also a number of proteins in the blood that play an important role in our bodies, of which about 90% are made in the liver. In addition, the liver acts as a detoxification function and immune organs, and various drugs and harmful substances entering our body are converted into less harmful substances in the liver and excreted through urine or bile. In particular, the liver has an immune cell called Cooper cells to break down bacteria and toxins or foreign substances coming out of the body through phagocytosis and to be discharged out of the body.

As such, the liver performs a variety of important functions, if the liver function is severely degraded, various problems occur. However, the liver has a vigorous regeneration, and even after 75% ablation, size and function are restored after 4-6 months.

Liver diseases include liver disease caused by viruses, alcoholic liver disease caused by excessive drinking, toxic liver disease caused by drugs, fatty liver in which fat accumulates in the liver, autoimmune liver disease caused by abnormalities in the human immune system, and toxicity. It is divided into metabolic liver disease caused by excessive accumulation of substances.

The main cause of chronic liver disease in Korea is hepatitis B virus and hepatitis C is on the rise, and it is relatively small compared with these viral liver diseases. However, alcoholic liver disease caused by habitual drinking and alcohol consumption is considerably increased recently. It is increasing.

Alcoholic liver disease is classified into fatty liver, alcoholic hepatitis, and alcoholic cirrhosis according to clinical and pathological findings. Alcoholic fatty liver is known to occur in 90-100% of habitual drinkers, alcoholic hepatitis in 10-35%, alcoholic cirrhosis in 8-20%.

Alcohol absorbed into the body is excreted in the lungs, urine and sweat by about 10%, 90% is metabolized in the liver. Alcohol, which is transported to the liver through the blood, is oxidized to acetaldehyde by various enzymes produced by the liver (Alcohol Dehydrogenase, Microsomal ethanol oxidizing system, Catalase, etc.), and acetaldehyde is again oxidized to acetic acid, which is harmless to the body by the enzyme.

Acetaldehyde, a metabolite produced by alcohol oxidation, is a highly reactive toxic substance that binds to proteins, inhibits enzyme activity, damages mitochondria due to increased lipids and oxidation, causes glutathione deficiency, and pyridoxine, vitamin A, zinc and selenium. It has been pointed out as a major factor causing liver damage that causes deficiency, inhibits protein secretion and migration by inhibiting tubulin polymerization. In addition, the production of free radicals by acetaldehyde promotes collagen synthesis in the liver, which is reported to cause liver fibrosis (cirrhosis) in chronic alcohol intake.

To this end, many materials such as alcohol metabolism inhibitors, alcohol metabolism promoters, alcohol degrading enzymes, and the like have been developed or utilized in the composition thereof. For example, Korean Patent Registration No. 0178696 discloses a pharmaceutical composition for liver protection and liver disease treatment. No. 0050333 Hangover drink and its manufacturing method for the hangover remedy and liver function improvement, Patent registration No. 0345798 Hangover functional foods using earthworm, alder, 칡 mixture and its manufacturing method, Korean Patent No. 2002-0021980 Alcohol metabolism enhancer and liver disorder lowering agent, hangover removal and soy sauce protective composition containing alcohol absorption inhibitor, there is a health food based on the earth and alder.

In addition, patent registration No.0403720 hepatotoxicity, hangover and anti-fatigue activity of low alcohol insoluble extract fractions and polysaccharides and compositions containing the same, hepatotoxicity and hangover isolated from No. 403721 Lower alcohol insoluble extract fraction and polysaccharide material having relieving activity and composition containing same, low alcohol insoluble extract having hepatoprotective activity isolated from woody part of patent document No. 0438522, polysaccharide isolated from it and composition containing same, There is a hangover composition and hangover releasing agent containing the same as an active ingredient, hangangpi extract, brown root extract, goji berry extract, alderwood extract, nectar extract, milk thistle extract is the main ingredient 5 containing turnip concentrate, green bean-rice bran fermentation extract as an additive Article containing an active ingredient is not yet actual circumstances insignificant to.

Therefore, the present invention was made in view of this point, the main components of the thorn oak extract, brown root extract, goji berry extract, alderwood extract, nectar extract, milk thistle extract as a main raw material and turnip concentrate, green bean-rice bran fermentation extract It contains five active ingredients to protect or heal liver damage caused by alcohol and drugs, and also to lower blood alcohol after drinking. It is intended to provide a new form of hepatic protection and blood alcohol breakdown that has a synergistic effect than when used individually.

The present invention is to contain five active ingredients as the main raw material and the turnip concentrate, green soybean-rice bran fermentation extract as a main ingredient among the thorn extract, goose root extract, goji berry extract, alderwood extract, nectar extract, milk thistle extract It features.

More specifically, among the thorny opi extract, brown root extract, goji berry extract, alderwood extract, nectar extract, milk thistle extract, three components accounted for 52% by weight as the main raw material, turnip concentrate, green bean-rice bran fermentation extract as 28% by weight. The remainder are compositions that are effective in protecting liver from liver and decomposing blood alcohol.

Here, Eleutherococcus senticosus ( Eleutherococcus senticosus) is a medicinal plant with excellent medicinal effects that is known as siberian ginseng as an elmaceae , in oriental medicine by drying roots and bark between summer and autumn. Prescribed, each, bruises.

The root is a perennial vine belonging to the legumes medicinal and edible root, which is used in herbal medicine for hypertension, coronary arteriosclerosis, angina pectoris, senile diabetes. Flavonoids of the roots are also known as inhibitors of alcohol dehydrogenase and mitochondrial dehydrogenase, and have lipid peroxidation inhibitory, antioxidant and interpolation effects.

The goji berry is a deciduous vine shrub belonging to the branch family, fruit is called Lycii fructose , the root is called Lycii cortex , and the leaves are called Lycii folium . It has been used for the prevention and treatment of various diseases such as weakness, chronic wasting disease, kidney and liver protection as herbal medicine. It also lowers blood sugar and cholesterol, lowers blood pressure, and protects liver tissue (Jin, Jin-Sum, Shin, Min-Kyo.

Here, the alder ( Alnus japonica Steud.) Is a deciduous algae tree in the birch family, also known as glass tree (楡 里木) or red sheep (赤 楊), and is called tea in China. It is known that the native Korean duck tree in the deep mountains must be used to loosen the poison and protect the liver. The main ingredients are tannins, the triterpenoids taraxerol, betulinic acid and essential oils. This tree is known as a herb that has excellent therapeutic effect against various liver diseases such as hepatitis, cirrhosis and fatty liver.

The nectar ( Prunella vulgaris var. Lilacina Nakai) is a toxic herbal medicine that has been harvested when fruit trees wither in half in summer and dried in the sun and used as medicinal herbs. In oriental medicine, it has been used for chronic boils, acute mastitis and lymph node tuberculosis. Major ingredients include saponins such as oleanolic acid and ursolic acid, carotene, vitamin C, vitamin K, tannin, and caffeic acid. , Chlorogenic acid and the like are known [Korea Plant Research Resources Review, Korea Research Institute of Chemical Technology, pp.480-482 (1988)].

The milk thistle ( Silybum marianum (L.) Gaertner) is an asteraceae plant that is native to the Mediterranean region of 1 year or 2 years, and has been widely used as a medicinal plant in Europe. Today, the plant's purified extract, silymarin, or its main ingredient, silybin, is being used as an adjuvant treatment for toxic liver damage, chronic inflammatory liver disease, viral hepatitis, and cirrhosis. In addition, hepatic regeneration by increasing protein synthesis, inhibits the formation of leukotriene and inflammatory prostaglandin produced during liver damage, strong antioxidant effects, glutathione, an essential ingredient for detoxification after drinking It shows various biological activities such as prevention of deficiency.

The green bean-rice bran fermentation extract is a natural product obtained by fermentation of unroasted coffee beans and grains by a fermentation method using microorganisms. Inositol phosphate, polyphenols, and amino acids (inositol phosphate) having an antioxidant action and harmful oxygen removal effect It is a functional plant fermentation extract containing acid) and peptide, which removes harmful oxygen that is harmful to the human body to prevent DNA damage (cause of cancer), to prevent peroxidation of unsaturated fatty acids, and to prevent hypertension due to ACE inhibition. There is.

In addition, as an active ingredient of the turnip ( Brassica campestris L. var. Rapifera ), there are sulfur-containing compounds isothiocyanate, disulfide, and sulfoaphane. The spicy flavor of turnips has long been known as an anticancer ingredient, and animal studies have proven its effectiveness in preventing esophageal, liver, lung and colon cancers. Recently, animal studies in Korea have been reported to reduce the effects of liver cancer and cirrhosis.

Hereinafter, the configuration of the present invention will be described in more detail by examples.

Example

First, the alder extract was extracted with 100 g of boiled water at 100 ° C. in 100 g of dried alder wood and bark for 3 hours, and then the extract was filtered through a 5 μm filter paper to finally obtain 500 g of the alder extract.

Prickly pear and root extract were put into 100 grams of dried thorny vine and root and extracted in 1000 g of boiling water at 100 ° C. for 3 hours, and then the extract was filtered through 5 μm filter paper to finally obtain 500 g of thorny stem and root extract.

After extracting 50 g of dried honey grass into 1000 g of boiling water at 100 ° C. for 2 hours, the extract was filtered through a 400 mesh to finally obtain 600 g of honey leaf extract.

The wolfberry was put 100 g of dried wolfberry in 1000 g of boiling water at 100 ℃ extracted for 2 hours, and then the extract was filtered through a 400 mesh (600 mesh) to finally obtain 600 g of wolfberry extract.

In addition, milk thistle extract (TGS, Japan) was purchased in the market that the total flavonoid content of more than 80% in powder form, and green soybean-rice bran fermentation extract (Toyo Hakko, Japan) was purchased from the market used liquid , Turnip concentrate [Ganghwa Co., Ltd.] was used as a liquid (35 ^o Brix) commercially available.

The components obtained in this way are mixed and stirred, but the three components are 52% by weight as the main raw material among the barberry extract, brown root extract, goji berry extract, alderwood extract, nectar extract or milk thistle extract with respect to the total weight. A composition (named Y-MIX) effective for protecting the liver and blood alcohol degradation of the present invention containing 28% by weight of rice bran fermented extract as an auxiliary material and 20.0% of water contained the rest.

Hereinafter, the present invention will be described in more detail with reference to test examples.

The experimental animals used in the test examples of the present invention were 5 weeks old Sprague-Dawley (SD) male rats (Korea Biolink Co., Ltd.) at room temperature 25 ± 1 ° C. and humidity 50 ± 5% solid conditions. It was preliminarily bred for one week with feed (manufactured by CJ Corporation) and then used for testing.

Test Example 1 (Protection of Rat Hepatocytes by Carbon Tetrachloride)

In order to determine the effect of protecting the hepatocytes of the experimental animals against carbon tetrachloride when the composition components of the present invention were administered alone or in combination, the following experiments were carried out. Isolation and culture of rat liver cells were performed by Berry and Friend [High-yield preparation of isolated rat liver parenchymal cell. J. Cell. Biol. 43: 506-520 (1969)].

(Isolation of Liver Cells from Rats)

Rats were anesthetized with urethane (urethane, 1 g / kg) and then the stomach was disinfected with 70% (v / v) alcohol. After the abdomen's central line was cut and both sides were cut, a 20 gauge catheter was inserted into the hepatic portal vein to perfuse Hanks' Balance Salt Solution (HBSS, GIBCO) at a flow rate of 15-20 ml / min. While the inferior vena cava was cut under the renal vein to remove blood. After about 10-20 minutes, the inferior vena cava is added to the relative vein while perfused with a Hank balance salt solution containing 0.05-0.06% (w / v) collagenase (Sigma). Intubation caused excess fluid to pass through the liver tissue. The Hank balance salt solution was allowed to continue flowing through the Hank balance salt solution for 20 minutes to cause the liver to swell, and then the capsules were broken with sterile scissors. Hepatocytes were freed and filtered with sterile gauze. The filtrate was centrifuged twice at 1,500 rpm for 3 min to wash the hepatocytes, followed by 10% fetal bovine serum (Gibco), 1% penicillin-streptomycin (Gibco), and fungi zone (Fungizone). Gibco)] 2.5 ml / 500 ml, and glutamine [L-Glutamin (Sigma)] 200 mM added William medium E [Williams' Media E (1X, GIBCO)]. Cell viability was diluted with trypan blue (GIBCO) and counted with a haematometer.

(Cultivation of rat liver cells and induction of carbon tetrachloride toxicity)

Hepatocytes were diluted to 1.0 × 10 ⁶ cells / ml in William Medium E and 0.5 ml aliquoted into 24 well plates precoated with 20 μg rat tail collagen (Sigma). After 4 hours of incubation at 37 ° C. in a 5% CO ₂ /95% incubator, the cells were replaced with fresh medium to remove adherent, damaged or dead cells. After exchange with fresh medium, carbon tetrachloride was added to the hepatocyte suspension to a final concentration of 5 mM to induce toxicity. mg / ml was added. After incubation for 12 hours at 37 ° C, 5% CO ₂ /95% incubator, hepatocyte culture medium was taken, centrifuged at 10,000 rpm for 3 minutes, and the GOT and GPT contents in the supernatant were measured using UV- It was measured by the rate method. Relative Protection of GOT and GPT (%) (Kang Hyun-Jung, Eun-Joo Lee, Sang-Hyun Sung, Young-Joong Kim, Eun-Sook Song, Mi-Jung Park, Heum-Sook Lee. 2003. Protection of Liver Toxicity of Mugwort. Was measured as follows.

Relative Protection of GOT (%)

   = 100 × [(GOT reference-GOT sample] / [(GOT reference-GOT control)

GOT reference: GOT level of rat hepatocyte culture medium containing carbon tetrachloride only

GOT sample: GOT values of carbon tetrachloride and bramble extract, root root extract, goji berry extract, alder extract, nectar extract, milk thistle extract, turnip concentrate, green soybean-rice bran fermentation extract or rat hepatocyte culture sample to which the composition of the present invention was added

GOT control: GOT level of rat hepatocyte culture medium without carbon tetrachloride

Relative Protection of GPT (%) was calculated as GPT instead of GOT.

The results are shown in Figure 1, as shown in the drawing, the composition of the present invention, prickly pear extract, brown root extract, goji berry extract, Alderwood extract, Lamiaceae extract, milk thistle extract, turnip concentrate, green bean-rice bran fermentation extract individually It can be seen that there is a synergistic effect than when used.

Test Example 2 (lower blood alcohol in the composition)

Animal administration of the blood alcohol lowering effect when the composition of the present invention was administered as follows. Negative control group and positive control group were administered 5 ml / kg BW forced oral PBS (Phosphate buffered saline) for 2 weeks, the composition group was administered the composition of the present invention 5 ml / kg BW for 2 weeks, each group was 5. On the 15th day of the test, PBS was administered to the negative control group and the positive control group, and 5 ml / kg BW was administered to the composition group, respectively. Within 30 minutes, 4 g / kg of alcohol was added to the positive control group and the composition group with 22% alcohol solution. BW forced oral administration. After alcohol administration and then a predetermined time has elapsed added HEPA children taking the 100 ㎕ blood by the treated capillary here dichloromethane (dichloromethane) (Sigma) in 1 ml on from US vein after vigorous stirring a small amount of anhydrous Na ₂ SO ₄ was treated to remove water, filtered and used for blood alcohol content analysis. After alcohol administration, blood was collected by time to measure the amount of alcohol in the blood. Blood alcohol content was used by gas chromatography (Hewlett Packard HP6890, GC), and the sample was injected into GC using an autosampler (HP G1512A autosampler). The detector used a flame ionization detector (FID) and nitrogen as the mobile phase gas. The column used for analysis was DB-Wax (30 m × 0.25 mm × 0.15 μm; Agilent), and the analysis conditions were maintained at 55 ° C. for 2 minutes at 200 ° C., temperature programming, and 10 minutes. Increasing by ℃ / min was maintained for 4 minutes at 70 ℃. Ethyl acetate was used as an internal standard.

The results are shown in FIG. 2, and as shown in the drawing, the composition of the present invention showed excellent blood alcohol lowering ability as compared to the control group.

Test Example 3 (protective effect of liver damage caused by excessive alcohol intake of the composition)

In order to determine the hepatoprotective effect on hepatotoxicity caused by excessive alcohol intake when administering the composition of the present invention, animal experiments were carried out as follows. Negative control and positive control group were treated with PBS (Phosphate buffered saline) 5 ml / kg B.W. Forced oral administration was performed for 2 weeks, and the composition group contained 5 ml / kg B.W. Two weeks, each group was five. On day 15 of the test, 5 ml / kg B.W. Within 30 minutes after administration, 3 g / kg B.W. alcohol was added to the positive control and composition groups with 22% alcohol solution. Forced oral administration. After 6 hours of alcohol administration, heart blood was collected and blood GOT, GPT, and Bilirubin contents (IU / L) were measured using a blood analyzer (automatic analyzer 767, Hitachi, Tokyo, Japan). Modified Hepatic Activity Index (Nakaji, M., Hayashi, Y., Ninomiya, T., Yano, Y., Yoon, S., Seo, Y., Nagano) , H., Komori, H., Hashimoto, K., Orino, A., Shirane, H., Yokozaki, H., Kasuga, M. 2002.Histological grading and staging in chronic hepatitis: its practical correlation.Pathol.Int 52: 683-690).

The results are shown in Figure 3, it can be seen the excellent liver damage protection effect of the composition of the present invention.

Test Example 4 (protective effect of liver damage caused by chronic alcohol intake of the composition)

In order to investigate the protective effect of liver damage caused by chronic alcohol intake when the composition of the present invention was administered, animal experiments were carried out as follows. Negative control group and positive control group were treated with PBS (Phosphate buffered saline) for 5 ml / kg B.W. Forced oral administration was performed, and the composition group contained 5 ml / kg B.W. Administration was for 1 week. 2 g / kg B.W. alcohol with 22% alcohol solution in the positive control and composition groups within 30 minutes after PBS or Y-MIX administration on the 1st, 4th and 7th day of the test. Forced oral administration. After administration of alcohol on the 7th day of the test, heart blood was collected the next day to measure the contents of GOT, GPT, and Bilirubin in the blood. After necropsy, hepatic tissue was observed to measure the hepatic activity index expressed numerically of pathological symptoms. .

The results are shown in Figure 4, it can be seen the excellent liver damage protection effect of the composition of the present invention.

On the other hand, the composition of the present invention can be composed of various functional beverages, fermented milk foods, health supplements and the like by adding this as an active ingredient and a conventional carrier or excipient.

As described above, according to the present invention, by the three components as the main raw material from the extract of prickly pear extract, brown root extract, goji berry extract, alderwood extract, nectar extract, milk thistle extract and turnip concentrate, green bean-rice bran fermentation extract as a sub raw material, It has the function of protecting or healing liver damage caused by alcohol when ingested and at the same time lowering blood alcohol after drinking alcohol, in particular, protecting the liver damage caused by excessive drinking or chronic alcohol intake or healing the liver damaged by them. The effect is very excellent, and the function of promoting alcohol metabolism after drinking to lower the blood alcohol is very excellent, the use and application in related fields is expected.

Claims (5)

Alder tree extract, Lamiaceae extract and milk thistle extract accounted for 52% by weight as the main raw material, turnip concentrate and green soybean-rice bran fermentation extract as 28% by weight as a raw material, the rest of the composition consisting of water effective to protect the liver and blood alcohol. The method of claim 1, The alder extract is obtained by filtering the dried wood and bark of dried alder in boiling water for 3 hours and extracting it, The nectar extract is obtained by filtering the dried nectar into boiling water and extracting for 2 hours, The milk thistle extract is effective composition for liver protection and blood alcohol degradation, characterized in that the total flavonoid content is 80%. Fermented milk food containing the composition of claim 1 or 2 as an active ingredient Functional beverage containing the composition of claim 1 or 2 as an active ingredient Health supplement food containing the composition of claim 1 or 2 as an active ingredient

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