KR100655378B1 - Photosensitive sterilizer for microorganism and method of sterilizing microorganism using same - Google Patents

Abstract

Provided is a photosensitive sterilizer for microorganism comprising polyoxometalate showing strong microorganism sterilizing capacity due to excellent oxidizing property so as to be used for sterilizing the microorganism. The photosensitive sterilizer for microorganism comprises polyoxometalate which is at least one selected from the group consisting of H3PW12O40, H4SiW12O40 and H3Mo12O40P. The method for sterilizing microorganism comprises the steps of: (a) adding the photosensitive sterilizer for microorganism to the microorganism to prepare a mixture; and (b) applying light to the mixture.

Description

Microbial photosensitive sterilizer and microbial sterilization method using the same {PHOTOSENSITIVE STERILIZER FOR MICROORGANISM AND METHOD OF STERILIZING MICROORGANISM USING SAME}

1 is a schematic cross-sectional view showing a sterilization apparatus used in the present invention.

2 is a graph showing E. coli sterilization using titanium dioxide of Comparative Example 1.

Figure 3 is E. coli using the polyoxometallate microbial photosensitive fungicide of Examples 1 to 3 of the present invention It is a graph showing sterilization.

[Industrial use]

The present invention relates to a microbial photosensitive sterilizer and a microbial sterilization method using the same, and to a microbial photosensitive sterilizer having an excellent oxidizing power and a microbial sterilizing power, and a microbial sterilization method using the same.

[Prior art]

In order to purify water quality and air, a sterilizer is essential. In particular, a water purifier or an air purifier is required to remove microorganisms that cause water or air pollution.

Among various methods of removing microorganisms, a method using an oxide such as TiO ₂ , SiO _2, or ZnO is recently mentioned. Among these, titanium dioxide (TiO ₂ ) is recently used a lot, the action is as follows.

That is, titanium dioxide (TiO ₂ ) is a nano-sized fine particles, and when light is received, electrons and holes are generated on the TiO ₂ surface. The generated electrons and holes diffuse and move to the particle surface, and water or OH ⁻ adsorbed on the TiO ₂ surface reacts with the hole to generate a strong oxide material (eg, OH radical, superoxide). These oxides decompose organic substances and the like in water.

The OH radicals generated by the reaction as described above have a sterilizing effect of removing microorganisms, and are used as water quality or air purifiers. However, such titanium dioxide has a disadvantage of slow sterilization rate.

It is an object of the present invention to provide a microbial photosensitive fungicide capable of increasing the sterilization rate.

Another object of the present invention is to provide a microbial sterilization method using the sterilizing agent.

In order to achieve the above object, the present invention provides a microbial photosensitive fungicide comprising a polyoxometalate.

The present invention also provides a microbial sterilization method comprising the step of preparing a mixture by adding a photosensitive bactericide comprising polyoxometalate to the microorganism, and irradiating the mixture with light.

Hereinafter, the present invention will be described in more detail.

The terms, techniques, and the like described in this specification are used in the meanings generally used in the technical field to which the present invention belongs unless there is a specific limitation.

The present invention relates to a microbial photosensitive sterilizer for use in a sterilization apparatus used for the purification of water quality or air.

Microbial photosensitizing agents of the present invention include polyoxometalate. As the polyoxometalate, it is preferable to use one selected from the group consisting of H ₃ PW ₁₂ O ₄₀ , H ₄ SiW ₁₂ O _40, and H ₃ Mo ₁₂ O ₄₀ P, because it is most economical and easy to use. Among them, H ₃ Mo ₁₂ O ₄₀ P is the most preferable because it has the best microbial sterilization effect.

The photosensitive sterilizer including the polyoxometallate of the present invention has different physical properties such as solubility in water from the photosensitive sterilizer of the present invention, and is superior to TiO ₂ , which is known to have a microbial fungicide effect. The bactericidal effect is superior to that of silica-immobilized polyoxometalate, which exhibits a mechanism of action similar to TiO ₂ .

In the method for sterilizing microorganisms using the photosensitive sterilizer of the present invention, first, a photosensitive sterilizer including polyoxometallate is added to the microorganism. As the microorganism, anything that generally inhabits water or air may be sterilized in the present invention, and examples thereof include Escherichia coli and Bacillus.

The amount of the photosensitive bactericide including the polyoxometallate in the addition process may be adjusted according to the type of the polyoxometallate used, and the concentration of H ₃ PW ₁₂ O ₄₀ is preferably 0.05 to 2 mM per 10 ⁸ cfu / ml of the microorganism. The concentration of H ₄ SiW ₁₂ O ₄₀ is preferably 0.05 to 0.5 mM per 10 ⁸ cfu / ml of the microorganism, and the concentration of H ₃ Mo ₁₂ O ₄₀ P is preferably 0.05 to 0.5 mM per 10 ⁸ cfu / ml of the microorganism.

Subsequently, the obtained mixture is irradiated with light. At this time, the irradiation process of light is irradiated with light having a wavelength of 300 to 400nm and the irradiation time is preferably carried out for 10 to 40 minutes.

According to the light irradiation process, the photosensitive sterilant removes microorganisms due to the photooxidation reaction.

Hereinafter, preferred examples and comparative examples of the present invention are described. However, the following examples are only preferred embodiments of the present invention and the present invention is not limited to the following examples.

Device used :

1 shows a microorganism sterilization apparatus. As shown in FIG. 1, the microorganism sterilizing apparatus is provided with a UV lamp (1), a photosensitive sterilization reactor (2), a photosensitive sterilizer and a microorganism, which are located inside the photosensitive sterilizer to supply energy for activating the photosensitive sterilizer. A stirrer 3 for mixing well.

Preparation Example 1 Microbial Culture for Sterilization Experiment

For strain culture used in digestion reaction TSB (17 g / l casein, 3 g / l soybean meal, 2.5 g / l dextrose, 5 g / l sodium chloride, 2.5 g / l dipotassium phosphate) medium was used.

E. coli (KCTC 2571) was inoculated in 20 ml TSB medium in a 50 ml sterile tube and incubated at 37 ° C. for 15 hours, followed by centrifugation at 4,000 rpm for 10 minutes to remove the medium.

(Reference Example 1)

The cells produced in Preparation Example 1 were washed twice using 20 ml of sterile distilled water.

The washed cells and H ₄ SiW ₁₂ O ₄₀ polyoxometalate were mixed and stirred with a stirrer. At this time, the initial concentration of the bacterium Escherichia coli was used 3 × 10 ⁸ cfu / ml, the polyoxo metalate concentration was 0.1mM. The reaction time was 20 minutes.

(Reference Example 2)

The same procedure as in Reference Example 1 was repeated except that H ₃ Mo ₁₂ O ₄₀ P was used at a concentration of 0.05 mM as the polyoxometalate.

(Reference Example 3)

The same procedure as in Reference Example 1 was carried out except that H ₄ SiW ₁₂ O ₄₀ was used at a concentration of 0.05 mM as the polyoxometalate.

(Reference Example 4)

The same procedure as in Reference Example 1 was repeated except that H ₄ SiW ₁₂ O ₄₀ was used at a concentration of 0.2 mM as the polyoxometalate.

(Reference Example 5)

The same procedure as in Reference Example 1 was repeated except that H ₄ SiW ₁₂ O ₄₀ was used at a concentration of 0.35 mM as the polyoxometalate.

(Reference Example 6)

The same procedure as in Reference Example 1 was repeated except that H ₄ SiW ₁₂ O ₄₀ was used at a concentration of 1 mM as polyoxometalate.

(Reference Example 7)

The same procedure as in Reference Example 1 was repeated except that H ₃ Mo ₁₂ O ₄₀ P was used as a polyoxometalate at a concentration of 0.1 mM.

(Reference Example 8)

The same procedure as in Reference Example 1 was repeated except that H ₃ Mo ₁₂ O ₄₀ P was used as a polyoxometalate at a concentration of 0.5 mM.

(Reference Example 9)

The same procedure as in Reference Example 1 was repeated except that H ₃ Mo ₁₂ O ₄₀ P was used at a concentration of 1 M as the polyoxometalate.

(Reference Example 10)

The cells obtained in Preparation Example 1 were buffered with PBS (8 g / L sodium chloride, 0.2 g / L potassium chloride, 1.44 g / L sodium phosphate monobasic, 0.24 g / L potassium phosphate monobasic) Washed twice.

Then, the washed cells and titanium dioxide were mixed and stirred with a stirrer. At this time, the initial concentration of the bacterium Escherichia coli was used 5 X 10 ⁷ cfu / ml, the concentration of titanium dioxide was 3g / ℓ.

As an experiment to determine whether it acts as a photosensitizing fungicide, in order to determine the concentration at which microorganisms do not die in the absence of light, the bactericidal power according to the concentration of the polyoxometallate photosensitive fungicides according to Reference Examples 1 to 10 was measured. The results are shown in Table 1 below. The bactericidal power is a value measured after 20 minutes of sterilization reaction. In Table 1, "-" in Reference Examples 6 and 9 means that all of the microorganisms are sterilized.

Concentration (mM) E. coli (log (N / No) Control H ₄ SiW ₁₂ O ₄₀ 0 0 Reference Example 3 0.05 0 Reference Example 1 0.1 -0.55 Reference Example 4 0.2 -3.7 Reference Example 5 0.35 -4.7 Reference Example 6 One - Control H ₃ Mo ₁₂ O ₄₀ P 0 0 Reference Example 2 0.05 -0.0036 Reference Example 7 0.1 -2.7 Reference Example 8 0.5 -4.3 Reference Example 9 One - Reference Example 10 TiO ₂ 3g / ℓ 0

As shown in Table 1, it can be seen that the polyoxometallate can obtain E. coli bactericidal effect when the concentration is increased even in the absence of light. Accordingly, a microbial sterilization experiment was used at low concentration. On the contrary, it can be seen that TiO ₂ has no bactericidal effect even when an excess of 3 g / L is used.

Comparative Example 1 Microbial Sterilization Using Titanium Dioxide (TiO ₂ )

The cells obtained in Preparation Example 1 were prepared with PBS (8 g / L sodium chloride, 0.2 g / L potassium chloride, 1.44 g / L sodium phosphate monobasic) and 0.24 g / L potassium phosphate monobasic buffer. Wash twice.

Then, the washed cells and titanium dioxide were mixed and stirred with a stirrer. At this time, the initial concentration of the bacterium Escherichia coli was used 5 X 10 ⁷ cfu / ml, the concentration of titanium dioxide was 1g / ℓ. Then the UV lamp was turned on and sterilized. At this time, the wavelength of the UV light source was used 365nm. The reaction time was reacted up to 40 minutes at 10 minute intervals.

Example 1 Microbial Sterilization Using Polyoxometallates (POMs)

The cells produced in Preparation Example 1 were washed twice using 20 ml of sterile distilled water.

The washed cells and H ₃ PW ₁₂ O ₄₀ polyoxometalate were mixed and stirred with a stirrer. At this time, the initial concentration of the bacterium Escherichia coli was used 3 X 10 ⁸ cfu / ml, polyoxo metalate was 0.35mM. Then the UV lamp was turned on and sterilized. At this time, the wavelength of the UV light source was used 365nm. The reaction time was reacted up to 20 minutes at 5 minute intervals.

* Microbial count

After the completion of the sterilization reaction according to Comparative Example 1 and Examples 1 to 3, the plate of the reaction solution 100ml each of NA (3.0g / L beef extract, 5.0g / L peptone, 15g / L agar) plate 37 Incubation was carried out at 15 ° C. for 15 hours to determine the number of microorganisms. In addition, the microbial viable count was measured as a control group without the addition of a photosensitive bactericide for comparison.

The measured results of Comparative Example 1 are shown in FIG. 2 and the results of Examples 1 to 3 are shown in FIG. 3.

 The results were calculated by calculating the E. coli concentration (N) according to the sterilization reaction time against the initial E. coli concentration (N0) as a log value (log (N / N0)).

As shown in Figure 2, after 10 minutes through the measurement of the sterilization ability according to the reaction time of Comparative Example 1 -log1.3, 20 minutes, 30 minutes and 40 minutes -log2.0, -log2.8,-respectively It can be seen that the bactericidal power of about log3.5.

In contrast, Examples 1, 2, and 3 showed -log1, -log3.8, and -log3.3, respectively, after 5 minutes. After stopping the reaction for 20 minutes, the sterilization ability was much better than that of Comparative Example 1 using titanium dioxide as -log3, -log4.8, and -log5.2, respectively, among which H ₃ Mo ₁₂ O ₄₀ P was the most excellent sterilization. It was found to have an effect.

The present invention can utilize polyoxometallate as a photosensitizing bactericide for sterilizing action.

Claims (11)

Microbial photosensitive fungicide comprising polyoxometalate. According to claim 1, The polyoxometallate is at least one microbial photosensitive fungicide selected from the group consisting of H ₃ PW ₁₂ O ₄₀ , H ₄ SiW ₁₂ O ₄₀ and H ₃ Mo ₁₂ O ₄₀ P. The method of claim 2, The polyoxometalate is H ₃ Mo ₁₂ O ₄₀ P microbial photosensitive fungicide. Adding a photosensitive bactericide comprising polyoxometalate to the microorganism to prepare a mixture; To irradiate the mixture with light Microbial sterilization method comprising the process. The method of claim 4, wherein The polyoxometalate is at least one selected from the group consisting of H ₃ PW ₁₂ O ₄₀ , H ₄ SiW ₁₂ O ₄₀ and H ₃ Mo ₁₂ O ₄₀ P microbial sterilization method. The method of claim 5, The polyoxometalate is H ₃ Mo ₁₂ O ₄₀ P microbial sterilization method. The method of claim 4, wherein The photosensitizing sterilizing agent is H ₃ PW ₁₂ O ₄₀ , the amount of the photosensitizing sterilizing agent is a microbial sterilization method of 0.05 to 2mM concentration per 10 ⁸ cfu / ml. The method of claim 4, wherein The photosensitizing sterilizing agent is H ₄ SiW ₁₂ O ₄₀ , the amount of the photosensitizing sterilizing agent is a microbial sterilization method of 0.05 to 0.5mM concentration per 10 ⁸ cfu / ml microorganisms. The method of claim 4, wherein The photosensitizing sterilizing agent is H ₃ Mo ₁₂ O ₄₀ P, the addition amount of the photosensitizing sterilizing agent is a microbial sterilization method of 0.05 to 0.5mM concentration per 10 ⁸ cfu / ml. The method of claim 4, wherein The light microbial sterilization method having a wavelength of 300 to 400nm. The method of claim 4, wherein The irradiation step is a microbial sterilization method is carried out for 10 to 40 minutes.

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