KR100650494B1 - Composition containing guaiacol derivatives and syringol derivatives extracted from natural plant vinegar - Google Patents

Abstract

The present invention relates to a composition containing a guai alcohol-based component and a syringol-based component extracted from wood vinegar as an active ingredient. The present invention provides a composition which is not only safe, but also has an excellent blood circulation improving function and a stool relieving function. The composition of the present invention can be usefully used as a raw material of a pharmaceutical or dietary supplement.

Guai alcohol-based ingredients, syringol-based ingredients, wood vinegar, blood circulation improvement, hangover relief

Description

Composition containing guaiacol derivatives and syringol derivatives extracted from natural plant vinegar}

1 is a diagram illustrating an example of a guai alcohol-based component extracted from wood vinegar.

2 is a diagram showing an example of a siring callo component extracted from wood vinegar solution.

Figure 3a is a diagram showing the results for the composition of the present invention containing a guai alcohol-based component and a siringol-based component in the test for platelet aggregation using thrombin.

Figure 3b is a diagram showing the results of the composition of the present invention containing a guai alcohol-based component and a siringol-based component in the platelet aggregation evaluation experiment using collagen.

Figure 4a is a diagram showing the results for the composition of the present invention containing guai alcohol-based components, siringol-based components and green tea leaf extract in the evaluation of platelet aggregation using thrombin.

Figure 4b is a diagram showing the results of the present invention composition containing guai alcohol-based components, siringol-based components and green tea leaf extract in the platelet aggregation evaluation experiment using collagen.

The present invention relates to a composition having a blood circulation improvement function and hangover relief function containing the components extracted from wood vinegar as an active ingredient.

A thrombus is a clump of blood that clumps up inside a blood vessel and is said to have a detrimental effect on the cardiovascular system by preventing blood circulation. Cardiovascular disease is the leading cause of death worldwide, and cardiovascular disease is the number one cause of death in Korea.

Blood coagulation mainly involves plasma cells (platelets) and blood cells (red blood cells), and these tissues maintain homeostasis of blood flow and maintain normal hemostasis and protection at damaged or inflammatory sites of blood vessels. Maintain normal function. However, excessive activation of coagulation factors in the plasma, acceleration of platelet aggregation, abnormal erythrocyte deformability, and the like destroy the homeostasis of blood flow and cause cardiovascular diseases such as blood circulation disorders such as arteriosclerosis and stroke. In normal blood vessels, homeostasis is maintained by balancing the inhibitory response with the activation response of the hemostatic mechanism. However, excessive hemostasis and the formation of clots interfere with the flow of blood, leading to abnormal blood circulation and causing lesions such as blood clots.

Modern people drink a lot of alcohol as part of their social activities. Alcohol is the main component of alcohol, ingested alcohol is mainly converted to acetaldehyde (oxidation) in the liver, and some (about 10%) is released into the breath, urine and sweat. The conversion of acetaldehyde in the liver is known to proceed by three reactive enzyme systems: alcohol dehydrogenase (ADH), microsomal ethanol-oxidizing system (MEOS) and catalase. Acetaldehyde, which occurs during alcohol metabolism, is known to cause a hangover that causes heavy head, general boredom, fatigue, bloating and vomiting after drinking. In addition, people with severe hangover symptoms had higher acetaldehyde levels than those with low hangover symptoms, but there was no report of differences in the concentration of ethanol in the blood, and the cause of alcohol-induced hangover is focused on acetaldehyde.

On the other hand, the wood is piled up in a place where there is little air such as a tang kiln (charcoal kiln) and the fire is fired. When the temperature inside the tangama reaches 350-450 ℃, white smoke comes out and the wood becomes charcoal. In this process, when the smoke discharged through the stack of tangama is collected in the house research and passed through the cooling stack, brown water droplets are generated by condensation. . This crude wood vinegar is placed in an acid-resistant container and allowed to stand in its natural state for six months to one year to separate into three layers. The uppermost layer is a light oil layer, the middle layer is wood vinegar, and the lower layer is a tar layer. Separation of this intermediate layer alone yields an effective conventional basal liquor solution of pH 3. Such basic wood vinegar includes organic acids such as formic acid, acetic acid and lactic acid; Phenol components such as phenol, cresol, 2,4-, and 3,5-kisylenol; Carbonyl compound components such as formaldehyde, acetaldehyde and propion aldehyde; And about 280 organic acids such as methanol and ethanol alcohol components, and 13 rare elements such as minerals and germanium.

Wood vinegar has been used since the Japanese Ming Dynasty and the Sino-Japanese War, but since its purpose was the production of acetic acid, the main ingredient of wood vinegar, acetic acid was then easily produced at low prices by synthetic chemistry. The use is almost gone. Although the use of wood vinegar was resumed before and after World War II, to date, its use is not the use of ingredients contained in wood vinegar, but uses the characteristic odor and color of wood vinegar as it is, for example, smoke odor of wood vinegar as it is. In the production of ham, bacon, sausage, etc. to replace the effects of smoking, or to produce a color such as grilled fish or meat, the method of use was very simple.

In Japan, attempts have been made to improve the symptoms of athlete's foot, atopic dermatitis, diabetes, hepatitis, etc., but the presence of harmful ingredients (tar, methanol, benzopyrene, methylcholene, etc.) contained in the wood vinegar is used in the human body. The study was very limited because no safety was established.

Wood grass liquor is a forest resource collected from the smoke of charcoal kilns. Wood vinegar is made by thermally decomposing cellulose, lignin, and the like contained in trees, and wood vinegar contains more than 200 kinds of organic components having various functions. However, wood vinegar contains not only active ingredients, but also harmful components such as tar, methanol, benzopyrene, and methyl cholensene, which have been suspected of safety for the human body and could not be recognized as a beneficial ingredient for health. In addition, the effective ingredients contained in the wood vinegar is not clearly identified and classified, it was not possible to predict the functional properties of the wood vinegar.

Therefore, the technical problem to be achieved by the present invention is to provide a composition for improving blood circulation function and / or hangover relief containing guai alcohol-based components and syringol-based components extracted from wood vinegar ensured safety for the human body.

In order to achieve the above technical problem, the present invention is a blood circulation improvement function and / or hangover relief containing a guai alcohol-based component represented by the formula (1) and a siringol-based component represented by the formula (2) below extracted from wood vinegar solution It provides a functional composition.

In Formulas 1 and 2 above, R is hydrogen, alkyl, oxoalkyl or alkenyl.

In another aspect, the present invention provides a composition for improving blood circulation function and / or hangover relief containing guai alcohol-based component represented by the above formula (1), siringol-based component represented by the formula (2) and green tea leaf extract extracted from wood vinegar solution .

As described above, the wood vinegar contains more than 200 kinds of organic components which are expected to have various functions, but at the same time, it contains a lot of harmful components such as tar, methanol, benzopyrene and the like. In addition, the active ingredients contained in wood vinegar have not been classified and identified, so the pharmacological effects of wood vinegar have not been revealed.

Therefore, the present inventors have developed a method for removing harmful components from wood vinegar using several methods (see Korean Patent Registration Nos. 0290986 and 0212472), and classified and identified active ingredients of wood vinegar purified in this way. . In addition, as a result of many studies to clarify the pharmacological and physiological effects of the classified and identified wood vinegar active ingredients, the composition of the present invention containing guai alcohol-based ingredients and syringol-based extracts extracted from wood vinegar is used for improving blood circulation and hangover. Invented that it can be used as.

In addition, the present inventors conducted various safety experiments on the composition of the present invention in consideration of the fact that the existing wood vinegar could not be used due to various side effects, through which it contains the guial alcohol-based component and the syringol-based component extracted from the wood vinegar It was confirmed that the composition of the present invention is safe for the human body.

Hereinafter, the present invention will be described in detail.

The present invention provides a composition for improving blood circulation and / or hangover function comprising a guai alcohol-based component represented by the following Chemical Formula 1 and a siringol-based component represented by the following Chemical Formula 2 below.

<Formula 1>

<Formula 2>

In Formulas 1 and 2 above, R is hydrogen, alkyl, oxoalkyl or alkenyl.

Examples of the guai alcohol-based components include guai alcohol, 4-methyl guai alcohol, 4-ethyl guai alcohol, 4-propyl guai alcohol, vanillin, 4- (2-propio) -vanillone ( 4- (2-propio) -vanillone), 4- (1-propio) -vanillone, eugenol, E-isoeugenol, Z-isoeugenol, acetovanilone and the like. The general formula is shown in FIG. 1.

Examples of the syringol-based component include syringol, 4-methylsilingol, 4-ethylsilingol, 4-propylsilingol, syringaldehyde, 4- (2-propio) -syringcon (4- (2 -propio) -syringone), 4- (1-propio) -syringone, 4- (2-propenyl) -syringol), E-4- (1-prop) Phenyl) -syringol, Z-4- (1-propenyl) -syringol, acetosyringone and the like. The chemical formula is summarized in FIG. 2.

In another aspect, the present invention provides a composition for improving blood circulation function and hangover relief containing guai alcohol-based component represented by the above formula (1), siringol-based component represented by the above formula (2) and green tea leaf extract extracted from wood vinegar solution.

Preferably, the composition of the present invention containing a guai alcohol-based component, a syringol-based component and green tea leaf extract extracted from wood vinegar is a combination of the guai alcohol-based component and a siringol-based component based on the total weight of the composition 10 ^-6 to 95 It contains by weight, and contains 0.01 to 30% by weight green tea leaf extract. More preferably, the composition of the present invention contains nine children alcohol component ^10-6 to 90% by weight, siring kolgye components ^10-6 to 90% by weight, and green tea leaf extract, of 0.01 to 30% by weight. The composition of the present invention has the most preferable blood circulation improvement and hangover relief in the case of having the above content.

Green tea leaf extract contains polyphenolic compounds such as epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC) and epigallocatechin gallate (EGCG). These polyphenol compounds are known to have various effects such as inhibiting the action of free radicals involved in various diseases, inhibiting mutagenicity of carcinogens, inhibiting cholesterol reuptake, and antibacterial and antiviral effects.

The present invention also provides a surprising fact that when the green tea leaf extracts having such a therapeutic effect are used in combination with guai alcohol-based ingredients and syringol-based components extracted from wood vinegar, blood circulation improvement and hangover relief are greatly increased.

While most of the commonly used pharmaceutically active ingredients have the problem of being unstable to heat and thus cannot be heated in the manufacturing process, the guial alcohol compound and the syringol compound of the present invention are compounds extracted from wood vinegar, and extraction Wood vinegar used for the purpose is that the material obtained through the thermal decomposition of the tree has the advantage of being very stable to heat.

Classification and identification of the active ingredient in purified wood vinegar can be carried out by methods commonly used in the pharmaceutical field. For example, a classification method using a column, an extraction method using an organic solvent, or the like may be used. More specifically, the classification of the active ingredient in the purified wood vinegar can be classified using an organic solvent such as ether. In one preferred embodiment of the present invention, the active ingredient of purified wood vinegar can be obtained by acid and alkali treatment of the extract using an organic solvent such as ether. Identification of the active ingredient in purified wood vinegar can be performed using GC-MSD.

Phenolic fractions among acidic fractions, phenolic fractions, neutral fractions and basic fractions in purified wood vinegar have improved blood circulation and hangover. Useful active ingredients of the phenolic fractions include guiacol and syringol components, and these active ingredients are expected to have improved blood circulation and hangover. Guai alcohol and syringol-based components are volatile polyphenolic compounds that cause the characteristic odor of purified wood vinegar.

The blood circulation improvement effect of the composition of the present invention can be evaluated using experimental methods commonly used in the field to which the present invention belongs. For example, it may be evaluated by the effect on platelet aggregation induced by thrombin, collagen, or the like, or by the effect on serotonin secretion. In the endogenous coagulation pathway, when foreign substances such as collagen combine with blood coagulation factors in the blood to destroy platelets, thrombokinase contained in platelets is released, and thrombokinase and calcium ions cooperate to thrombin prothrombin in plasma. Switch to This thrombin converts fibrinogen into fibrin, which binds to blood cells in the blood to produce blood clots. That is, by adding thrombin and collagen to the blood and evaluating the degree of platelet aggregation due to this, the effect of the composition of the present invention on blood circulation improvement can be seen. Serotonin is also stored in the vesicles of platelets. When platelets are activated by thrombin stimulation, serotonin is secreted out of platelets by fusion of vesicles and cell membranes to induce platelet activation and vasoconstriction. Therefore, the degree of serotonin secretion can be evaluated to evaluate the effect of improving blood circulation of the composition of the present invention. Excellent blood circulation improvement effect of the composition of the present invention containing a guai alcohol-based component and a syringol-based component extracted from wood vinegar by these two methods and the composition of the present invention containing a guai alcohol-based component, a siringol-based component and green tea leaf extract You can see that it has.

Hangover effect of the composition of the present invention can be evaluated using a method commonly used in the field of the present invention. For example, it can be assessed by measuring the concentration of acetaldehyde expected to be the cause of hangovers. In this way, the composition of the present invention containing guai alcohol-based components and syringol-based components extracted from wood vinegar and the composition of the present invention containing guai alcohol-based components, siringol-based components and green tea leaf extracts have excellent hangover removal effects. It can be confirmed.

Meanwhile, wood vinegar has not been used until now because its safety has been a problem. Therefore, the safety of the composition of the present invention comprising a guai alcohol-based component and a syringol-based component extracted from wood vinegar is a very important evaluation factor. Such safety may be used a safety evaluation method commonly used in the pharmaceutical field. For example, the human safety evaluation of the composition of the present invention may be used to evaluate the toxicity, such as acute toxicity test, genotoxicity test, subacute toxicity test.

The acute toxicity test can be carried out according to the General Toxicity Test Method of National Institute of Health and Safety Notice No. 94-3 'Toxicity Testing Standards for Drugs' and 'Toxicity Testing Standard Work Guideline' of the National Institute of Health Safety. , Weight change, anatomical pathology findings are evaluated. Through this acute toxicity test, the composition of the present invention is evaluated to be very safe. According to the results of the acute toxicity test, the composition of the present invention containing guai alcohol-based ingredients and syringol-based components extracted from wood vinegar shows no acute toxicity upon oral administration to mice, and the LD ₅₀ value is 5,000 mg / kg kg or more. It is believed that this dose is safe when administered orally at a dose up to 50 times the expected daily intake of the composition of the present invention.

The genotoxicity test can be evaluated according to the Genotoxicity Test Method of National Institute of Health and Safety Notice No. 94-3, Standards for Toxicity of Drugs, etc. For example, the genotoxicity test Salmonella Reverse mutation testing with typhimurium , chromosomal aberration testing with cultured mammalian cells, and micronucleus testing with bone marrow cells from rodents can be used. The composition of the present invention does not induce a reverse mutation in the test coverage concentration 62-5000ug / plate range in the reverse mutation test using S. typhimurium TA1535, TA1537, TA98, TA100, the test application concentration in the chromosomal aberration test using mammalian culture cells It does not cause chromosomal aberrations in the range of 1.25-5 mg / ml. In addition, micronucleus tests using rodents do not induce micronucleus in the test dose range of 1250-5000mg / kg. This result means that the composition of the present invention is a very safe material that does not exhibit genotoxicity.

Subacute toxicity assessment can be assessed according to National Institute of Health and Safety Notice No. 94-3 'Toxicity Test Standards for Drugs'. For example, the test substance was orally administered to ICR male and female mice at a dose of 0.5, 1.0, 2.5, 5.0 g / kg / day, six times a week for a total of 28 days, and observed death animals, general symptoms, and weight change during the administration period. Is evaluated. After final administration, gross necropsy findings, long-term weight measurements, hematologic and hematological biochemical tests, and histopathological examinations are performed. The composition of the present invention is determined to be safe in all the above evaluation items, the non-toxic amount of the composition of the present invention is confirmed to be 5.0g / kg / day or more.

The composition of the present invention comprising a guai alcohol-based component and a syringol-based component extracted from wood vinegar solution may further include conventionally used excipients, disintegrants, binders, lubricants, sweeteners, coloring agents, flavoring agents, etc. It may be formulated in unit dosage form or in several dosage forms such as tablets, capsules, powders, granules, suspensions, emulsions, syrups, solutions or parenteral preparations by conventional methods.

The composition of the present invention comprising a guai alcohol-based component and a syringol-based component extracted from wood vinegar solution can be administered orally according to the desired method, the composition of the present invention as an active ingredient per day 0.001 to 0.5g per 1kg body weight, Preferably, the amount of 0.01 to 0.2 g may be administered in one to several times. Dosage levels for a particular subject may vary depending on the subject's weight, age, sex, health condition, diet, time of administration, method of administration, rate of excretion, and extent of disease.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these examples are only intended to illustrate the present invention, the present invention is not limited to these embodiments.

Example 1 Classification and Identification of Functional Ingredients of Purified Wood Vinegar

Functional ingredient identification of purified wood vinegar includes HP-INNOWAX column (crosslinked polyethylene glycol, 30mm × 0.25mm (ID) × 0.25um (FT)) and HP-5MS (crosslinked 5% phenylmethylsilicone, 30mm × 0.25mm (ID) × 0.25um (FT)) column was used, and HP 5890 Series II Plus GC and 5972 MSD were used. The oven temperature was maintained at 50 ° C. for 2 minutes, then heated up to 220 ° C. per 3 ° C. per minute, and then at 220 ° C. for 5 minutes. The inlet temperature was 200 ° C., the detector temperature was 250 ° C., the flow rate of helium was 0.72 ml / min, and the split ratio was 10.

In the GC-MSD analysis, the oven temperature was maintained at 40 ° C. for 5 minutes, and then heated up to 220 ° C. at 3 ° C. per minute, and then maintained at 220 ° C. for 5 minutes. The flow rate of helium was 1 ml / min, and the split ratio was 50. The acceleration voltage was 70 eV, and most of the compounds were compared with commercial products or mass library data were used for estimation and identification.

Purified wood vinegar was extracted with an organic solvent and then the composition of the extract was investigated. 20 ml of wood vinegar was added to a 100 ml aliquot and extracted with ether. 5% NaHCO _{3 was} then added to the ether layer to separate the carbonyl portion from the aqueous layer. The separated aqueous layer was neutralized with 30% H ₂ SO ₄ and extracted with ether to obtain a carbonyl moiety. After extracting the carbonyl part, 2N NaOH was added to the remaining ether layer to separate the phenol part from the aqueous layer. The separated aqueous layer was neutralized in the same manner as the carbonyl portion extraction, and then extracted with ether to obtain a phenol portion. Thus, the carbonyl part and the phenol part were extracted, and the neutral part and the basic part were obtained from the remaining ether layer. All ether layers were concentrated under reduced pressure and weighed to calculate the content for each fraction therefrom. Thus, each extract was divided into acidic fractions, phenolic fractions, heavy component fractions and basic fractions by acid and alkali treatment, and then the components of each fraction were analyzed using GC and GC-MS. The results are shown in Table 1 below.

Fraction Fraction ratio (%) Component (Compound) ratio(%) Neutral fraction 24.8%  Piperonal 1.61  Coumarin 0.4  1-indanone 2.02  Not confirmed (N.D.) 95.97  Sum 100 Acidic fraction 25.3%  Acetic acid 13.44  Maltol 7.91  Acetophenone 1.57  Not confirmed (N.D.) 77.08  Sum 100 Phenolic Fraction 47.0  Guiacol 30.60  4-methyl guaiacol 0.60  4-ethyl guaiacol 1.91  4-propyl guaiacol 2.34  Vanillin 3.17  4- (2-propio) vanillone 0.11  4- (1-propio) vanillone 3.40  Eugenol 3.62  E-isoeugenol 0.21  Aceto-vanillone 1.45  Syringol 28.40  4-methyl syringol 4.47  4-ethyl syringol 2.66  4-propyl syringol 1.30  Syringaldehyde 1.49  4- (2-propio) -syringone 0.60  4- (1-propio) -syringone 0.23  4- (2-propenyl) -syringol (4- (2-propenyl) -syringol) 0.32  E-4- (1-propenyl) -syringol (E-4- (1-propenyl) -syringol) 0.83  Z-4- (1-propenyl) -syringol (Z-4- (1-propenyl) -syringol) 1.40  Acetosyringone 0.05  Not confirmed (N.D.) 10.84  Sum 100 Basic fraction 2.9  3-ethylphentane 37.93  4-methylene cyclohexanone 17.24  2,6-di-tert-butylquinone 27.59  Not confirmed (N.D.) 17.24  Sum 100 Sum 100

By fraction, the ratio of phenolic fraction (47%) was the highest, and that of basic fraction (2.9%) was the lowest. As a result of evaluation of blood circulation improvement and hangover ability using each fraction, it was confirmed that the effect of phenolic fraction was much superior to other fractions.

In the phenolic fraction, the ratio of the guai alcohol component and the siring alcohol component was 89.16%, which was the main constituent of the phenolic fraction. Guai alcohol-based components and syringol-based components are compounds produced by pyrolysis of guaninc and guanincyl units and syringyl units of lignin, which is a component of wood, Smoky aroma is also derived from these compounds. Based on these results, it was confirmed that the guai alcohol-based component and the siring-chol-based component were the functional ingredients of the purified wood vinegar solution.

Example 2 evaluation of blood circulation improvement effect

<Evaluation of Platelet Aggregation Induced by Thrombin and Collagen>

Composition of the present invention (test group 1, the same as below) and guai alcohol-based component 15.5 containing 15.5% by weight of guai alcohol-based component, 25% by weight of syringol-based component and residual water based on the total weight of the composition Evaluation of platelet aggregation induced by thrombin and collagen using the composition of the present invention (test group 2, the same as below) containing the weight percent, 25 wt% of siringol-based components, 0.5 wt% of green tea leaf extract and the remaining amount of water It was. Platelets play an important role in many vascular diseases by inducing excessive thrombus formation through activation and aggregation at damaged vascular sites (SiMinno and silver, 1983).

To determine the effects of Test Group 1 and Test Group 2 on platelets, platelets were prepared, and Test Group 1 and Test Group 2 were cultured with platelets in a concentration-dependent manner. Test group 1 and test group 2 were reacted with platelets at 37 ° C. for 10 minutes, and the control group using water did not change when the minimum unit or amount of thrombin or collagen that caused maximum aggregation was added. However, in the experimental group in which Test Group 1 and Test Group 2 reacted with platelets, aggregation by thrombin and collagen was inhibited in a concentration-dependent manner. The results are shown in FIGS. 3A, 3B, 4A and 4B, respectively. The degree of aggregation of platelets was measured as a change in turbidity using a lumi-aggregometer. The light transmittance when all the platelets aggregated is 100%, and the light transmittance when the platelets do not aggregate is 0%.

Based on these results, the IC50 for thrombin was 0.386% for test group 2 (N = 3) and 0.748% for test group 1 (N = 3). For collagen, IC50 was 0.207% for test group 2 (N = 3) and 0.547% for test group 1 (N = 3). As can be seen from these results, it was confirmed that the composition of the present invention inhibits platelet aggregation in a concentration-dependent manner in the evaluation of blood circulation improvement function using thrombin and collagen. In addition, test group 2 containing a guai alcohol-based component, a syringol-based component and green tea leaf extract showed a better effect than test group 1.

<Serotonin Secretion Assessment>

The composition of the present invention (test group 1) containing 15.5% by weight of guai alcohol-based component and 25% by weight of siringol-based component, 15.5% by weight of guai-alcohol-based component, 25% by weight of syringol-based component, 0.5% by weight of green tea leaf extract The effect of the composition of the present invention (test group 2) and the composition containing the green tea leaf extract 0.5% by weight (comparative group 1) on the secretion of serotonin was evaluated.

Test group 1, test group 2 and control group 1 were incubated with platelets for 10 minutes, and then 0.1 U / mL of thrombin was added. Thereafter, the amount of serotonin released for 3 minutes was quantified. Distilled water was used as a control. The results are shown in Tables 2, 3, and 4 below, respectively.

sample Serotonin Secretion (%) Average(%) 1 time Episode 2 3rd time Control 65.65 61.80 52.62 60.02 Test group 1-0.1% solution 63.65 60.04 46.20 56.63 Test group 1-0.5% solution 59.11 56.61 37.84 51.19 Test group 1-1% solution 39.77 46.07 17.09 34.31 Test group 1-2% solution 2.85 17.53 10.19 10.19

sample Serotonin Secretion (%) Average(%) 1 time Episode 2 3rd time Control 56.23 53.18 63.94 57.78 Test group 2-0.1% solution 56.66 47.88 61.41 55.32 Test group 2-0.5% solution 58.24 36.61 47.79 47.55 Test group 2-1% solution 34.86 14.54 32.14 27.18 Test group 2-2% solution 10.93 3.67 11.00 8.53

sample Serotonin Secretion (%) Average(%) 1 time Episode 2 3rd time Control 57.29 62.09 68.06 62.48 Comparative group 1-0.1% solution 61.75 59.98 62.23 61.32 Comparative group 1-0.5% solution 54.89 56.48 53.72 55.03 Comparative group 1-1% solution 45.78 47.21 46.45 46.48 Comparative group 1-1.25% solution 34.21 32.48 34.44 33.71 Comparative group 1-1.5% solution 21.54 23.41 22.79 22.58 Comparative group 1-2% solution 10.56 12.01 10.85 11.14

As can be seen from the results of Table 2, Table 3 and Table 4, the experimental results of the test group 1 and the test group 2 of the present invention inhibited the secretion of serotonin in a concentration-dependent manner by blocking the stimulation of platelets by thrombin . This means that the compositions of the present invention are useful for inhibiting thrombogenesis. In addition, the test group 1 showed an IC50 at a concentration of about 1%, but the test group 2 showed an IC50 at about 0.5%, and the comparative group 1 had an IC50 at a concentration of about 1.25%. This means that the composition of the present invention containing guai alcohol-based component, syringol-based component and green tea leaf extract is more preferable in blood circulation improvement effect, and that the composition of the present invention containing guai alcohol-based component and siring-chol based component If the extract is further contained, it means that a synergy effect occurs.

<Evaluation of Vasoconstriction Inhibitory Effect by Phenylephrine>

Composition of the present invention (test group 1) containing 15.5% by weight of guai alcohol-based component and 25% by weight of siringol-based component, 15.5% by weight of guai-alcohol-based component, 25% by weight of syringol-based component and 0.5% by weight of green tea leaf extract In order to evaluate the effect of the composition of the present invention (Test Group 2) and the composition containing 0.5% by weight green tea leaf extract (Comparative Group 1) on vascular contraction, the effect of inhibiting blood and contraction by phenylephrine was evaluated. It was.

The rat thoracic aorta was pretreated with 0.5 to 2% of Test Group 1 solution, 0.1 to 0.4% of Test Group 2 solution and control for 30 minutes, and then phenylephrine was added from low concentration to nodule. The results are shown in Tables 5, 6 and 7, respectively.

sample Dose-log [PE (M)] Shrinkage (%, 90mM K +) Average 1 time Episode 2 3rd time Control 9 1.34 -0.78 1.05 0.54 8.5 0.89 -1.75 -0.70 -0.52 8.0 1.12 -0.39 4.56 1.76 7.5 2.68 6.04 18.25 8.99 7.0 26.30 31.38 48.42 35.37 6.5 51.60 51.85 65.26 56.24 6.0 65.40 64.32 77.19 68.67 5.5 76.10 77.19 82.80 78.70 5.0 80.40 84.02 88.77 84.40 Test group 1-0.5% solution 9 -0.47 -1.53 1.69 -0.10 8.5 -3.04 2.68 1.69 0.44 8.0 0.23 4.02 4.22 1.49 7.5 1.41 9.39 11.81 7.54 7.0 21.50 40.23 47.68 36.47 6.5 46.40 56.70 66.67 56.59 6.0 62.50 71.84 79.75 71.36 5.5 70.70 83.33 90.30 81.44 5.0 74.50 89.46 98.31 87.42 Test group 1-2% solution 9 1.61 -0.50 -1.16 -0.02 8.5 0.00 -0.74 0.87 0.04 8.0 1.34 0.00 2.31 1.22 7.5 2.15 3.71 9.82 5.23 7.0 16.40 21.78 34.68 24.29 6.5 44.40 48.02 60.98 51.13 6.0 59.40 64.85 73.70 65.98 5.5 72.60 80.20 84.39 79.06 5.0 77.70 88.86 91.90 86.15

sample Dose-log [PE (M)] Shrinkage (%, 90mM K +) Average 1 time Episode 2 3rd time Control 9 0.90 -0.76 0.30 0.15 8.5 3.90 0.50 0.00 1.47 8.0 6.90 0.75 1.20 2.95 7.5 23.72 19.40 15.87 19.66 7.0 51.95 52.90 49.40 51.42 6.5 67.27 67.00 61.68 65.32 6.0 77.78 86.81 80.79 81.79 55. 84.38 90.43 85.82 86.88 5.0 89.19 92.95 90.90 91.01 Test group 2-0.1% solution 9 -0.45 -0.59 1.57 0.18 8.5 -0.45 0.29 2.83 0.89 8.0 0.00 0.30 2.89 1.06 7.5 4.23 2.37 5.03 3.88 7.0 18.26 18.34 16.35 17.65 6.5 35.63 23.96 33.33 30.97 6.0 44.54 39.65 45.91 43.37 5.5 54.12 44.38 54.72 51.07 5.0 58.13 48.22 55.97 54.11 Test group 2-0.2% solution 9 0.91 -0.31 0.00 0.20 8.5 0.23 0.61 0.55 0.46 8.0 0.68 0.61 0.82 0.70 7.5 0.23 2.14 1.10 1.16 7.0 0.23 3.98 4.93 3.05 6.5 8.9 22.94 18.63 16.82 6.0 29.00 34.86 29.31 31.06 5.5 34.58 40.57 39.45 38.20 5.0 38.81 44.95 45.21 42.99 Test group 2-0.4% solution 9 -1.39 -1.02 1.26 -0.38 8.5 -1.66 0.00 2.09 0.14 8.0 -1.19 2.03 2.93 1.26 7.5 0.28 2.37 3.77 2.14 7.0 3.40 7.46 3.77 4.88 6.5 20.78 17.29 16.66 18.24 6.0 27.42 29.83 22.55 26.60 5.5 29.36 34.57 27.99 30.64 5.0 30.75 41.02 28.41 33.39

sample Dose-log [PE (M)] Shrinkage (%, 90mM K +) Average 1 time Episode 2 3rd time Control 9 0.35 0.24 0.60 0.40 8.5 1.22 1.76 2.01 1.66 8.0 1.98 2.24 3.98 2.73 7.5 2.58 6.87 17.55 9.00 7.0 24.38 30.78 40.47 31.88 6.5 49.78 53.68 67.89 57.12 6.0 60.88 67.42 79.05 69.12 5.5 70.20 79.55 85.21 78.32 5.0 80.96 85.04 88.99 85.00 Comparative group 1-0.5% solution 9 0.11 0.44 0.55 0.37 8.5 0.89 2.45 3.65 2.33 8.0 2.99 3.56 7.89 4.81 7.5 8.57 10.02 15.55 11.38 7.0 27.88 39.89 45.02 37.60 6.5 69.45 70.02 65.78 68.42 6.0 75.45 75.00 78.81 76.42 5.5 78.98 80.21 79.95 79.71 5.0 90.01 85.49 84.02 86.51 Comparative group 1-2% solution 9 0.39 0.76 0.33 0.49 8.5 3.98 0.99 5.61 3.53 8.0 8.02 5.98 14.44 9.48 7.5 11.11 9.99 20.56 13.89 7.0 32.48 29.54 35.23 32.42 6.5 71.04 69.78 63.33 68.05 6.0 70.44 72.41 75.82 72.89 5.5 79.85 75.59 78.88 78.11 5.0 84.69 86.53 88.87 86.70

PE in Table 5, Table 6 and Table 7 represents phenylephrine (phenylephrine). Test Group 1 and Comparative Group 1 did not affect the contraction of blood vessels by phenylephrine (see Tables 5 and 7), whereas Test Group 2 containing guai alcohol-based components, syringol-based components and green tea leaf extracts Reduced the degree of contraction induced by phenylephrine in a concentration dependent manner (see Table 6). This means that the composition of the present invention containing a guai alcohol-based component, a syringol-based component, and green tea leaf extract has a more preferable effect on blood circulation improvement than Test Group 1 and Comparative Group 1.

Based on the above results, the composition of the present invention containing a guai alcohol-based component and a syringol-based component extracted from wood vinegar, and the composition of the present invention containing a guai alcohol-based component, a siringol-based component and a green tea leaf extract have a platelet aggregation inhibitory activity. And it was confirmed that it has an effect of inhibiting vasoconstriction. This means that the composition of the present invention can be used for improving blood circulation to improve blood circulation.

Example 3 Evaluation of Hangover Relief Effect of the Invention Composition

The concentrations of two representative toxic substances, ethanol and acetaldehyde, which occurred during alcohol consumption were measured by comparison with the control group over time. Distilled water was used as a control. In test group 1, 40 mg / kg of body weight of the composition of the present invention containing 15.5% by weight of guai alcohol-based component and 25% by weight of syringol-based component, and in test group 2, 15.5% by weight of guai-alcohol-based component and syringol-based 40 mg / kg body weight of the composition of the present invention containing 25% by weight of the component and 0.5% by weight of the green tea leaf extract, and Comparative Group 1 was administered a composition containing 0.5% by weight of green tea leaf extract.

Measurement of blood ethanol concentration change

Table 8 shows the concentration change of blood ethanol. The concentration of ethanol in blood was measured by the following method. After fasting the test animals for 18 hours, the test substance was prepared by oral administration in a solution of a suitable concentration. After 30 minutes, the alcohol is orally administered, and blood is collected from the heart for 1 hour, 3 hours, 5 hours orally, and 7 hours after administration. Serum was separated by centrifuging blood at 3000 rpm for 15 minutes, and then the amount of ethanol in serum was measured using an ethanol measurement kit (Ethanol, Roche, Swizerland).

sample 1 hour after administration 3 hours after administration 5 hours after administration 7 hours after dosing Area under time-concentration curve (AUC) Control 196.7 ± 25.2 180.7 ± 5.1 142.6 ± 26.1 99.3 ± 21.0 949.33 ± 108.74 Test group 1 47.2 ± 21.0 (p <0.05) 67.4 ± 4.2 (p <0.001) 39.1 ± 11.3 (p <0.05) 57.1 ± 31.9 (p <0.05) 398.65 ± 5.02 (p <0.05) Test group 2 144.7 ± 26.3 (p <0.05) 136.7 ± 20.8 (p <0.05) 100.0 ± 26.5 27.3 ± 13.0 (p <0.05) 655.00 ± 126.19 (p <0.05) Comparative group 1 187.7 ± 28.3 (p <0.05) 170.23 ± 18.8 (p <0.05) 130.40 ± 10.5 78.3 ± 23.0 (p <0.05) 876.68 ± 98.89 (p <0.05)

As can be seen from the results of Table 8, the test group 1 administered group reached the highest concentration of blood alcohol at 3 hours after alcohol administration, and was lower at all times than the control group. When the concentration of blood alcohol in the control group was 100%, test group 1 showed the greatest decrease by about 76% based on the concentration of blood alcohol in the control group after 1 hour of alcohol administration. The time decreased by 63%, 73% and 43%, respectively. There was a significant difference in all time zones.

In addition, when comparing the area under the curve (AUC) of blood ethanol of the control group, Test Group 1, Test Group 2 and Comparative Group 1, the time of blood ethanol of Test Group 1 compared to the control group The area under the concentration curve decreased by 58%, which was significantly lower (p <0.05). From these results, it is thought that the composition of the present invention containing the guai alcohol-based component and the syringol-based component reduces the concentration of elevated blood alcohol after alcohol administration.

<Measurement of change in acetaldehyde concentration in blood>

The measurement results are summarized in Table 9. Acetaldehyde concentration in blood was measured by the following method. After fasting the test animals for 18 hours, the test substance was prepared by oral administration in a solution of a suitable concentration. After 30 minutes, alcohol was orally administered, and blood was collected from the heart at 1 hour, 3 hours, 5 hours, and 7 hours after administration. Serum was separated by centrifuging blood at 3000 rpm for 20 minutes, and then the concentration of acetaldehyde in serum was measured using an acetaldehyde measurement kit (Acetaldehyde, Roche, Swizerland).

sample 1 hour after administration 3 hours after administration 5 hours after administration 7 hours after dosing Area under time-concentration curve (AUC) Control 0.30 ± 0.13 0.30 ± 0.09 0.39 ± 0.10 0.37 ± 0.08 2.05 ± 0.58 Test group 1 0.39 ± 0.09 0.27 ± 0.01 0.24 ± 0.06 0.15 ± 0.01 (p <0.05) 1.57 ± 0.24 Test group 2 0.17 ± 0.03 0.18 ± 0.07 0.11 ± 0.04 0.10 ± 0.05 (p <0.05) 0.86 ± 0.29 (p <0.05) Comparative group 1 0.26 ± 0.01 0.23 ± 0.05 0.24 ± 0.03 0.17 ± 0.02 (p <0.05) 1.85 ± 0.29

As can be seen from Table 9, the concentration of acetaldehyde in the control group was 0.37 ± 0.08 mg% after 7 hours of alcohol administration, and in the test group 1, the concentration of acetaldehyde in the blood group was 0.15 ± 0.01 mg 7 hours after alcohol administration. The percentage was significantly lower (p <0.05) than the control group and the comparative group 1. In addition, in test group 2, the acetaldehyde concentration in blood was 0.10 ± 0.05 mg% after 7 hours of alcohol administration, which was significantly lower than the control group, test group 1 and comparison group 1 (p <0.05). The AUC of acetaldehyde in blood was 2.05 ± 0.58 in the control group, 1.57 ± 0.24 in the test group 1, 0.86 ± 0.29 in the test group 2 (p <0.05) and 1.85 ± 0.29 in the comparative group 1, similar to the blood acetaldehyde concentration in the test group 2 At the lowest. This means that the composition of the present invention containing the guai alcohol-based component and the syringol-based component is effective in relieving hangover, and the composition of the present invention further containing green tea leaf extract has a synergistic effect in removing the hangover. Means that.

The present invention provides a composition for improving blood circulation and hangover function containing guai alcohol-based components and syringol-based components extracted from wood vinegar solution. More preferably, the present invention provides a composition for improving blood circulation and hangover function comprising guai alcohol-based component, syringol-based component and green tea leaf extract extracted from wood vinegar solution. The composition of the present invention is not only a safe composition without acute toxicity, genotoxicity, subacute toxicity, etc., but also has an excellent effect on blood circulation and hangover. The composition of the present invention can be usefully used as a raw material of the drug or health functional food for blood circulation improvement and hangover.

Claims (2)

Hangover relief function composition containing a guai alcohol-based component represented by the following formula (1) and a siringol-based component represented by the formula (2) extracted from wood vinegar. <Formula 1>

<Formula 2>

In Formulas 1 and 2, R is hydrogen, alkyl of 1 to 3 carbon atoms, oxoalkyl of 1 to 3 carbon atoms or alkenyl of 1 to 3 carbon atoms. According to claim 1, wherein the composition is a hangover relief composition, characterized in that it further comprises green tea leaf extract.

Images