KR100581356B1 - 싸이토메트리, 빌로시메트리 및 세포 분류에 사용하기위한 폴리전해질 염다리를 사용하는 마이크로칩 - Google Patents
싸이토메트리, 빌로시메트리 및 세포 분류에 사용하기위한 폴리전해질 염다리를 사용하는 마이크로칩 Download PDFInfo
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
Description
Claims (14)
- a) 시료 용액을 도입하는 입구,b) 상기 입구로부터 도입된 시료 용액의 이동통로를 제공하는 마이크로 채널,c) 상기 마이크로 채널을 통과한 시료의 적어도 하나 이상의 출구,d) 상기 마이크로 채널에 서로 대향하게 연결된 한 쌍의 제1 및 제2 폴리전해질 염다리와, 상기 제1 및 제2 폴리전해질 염다리에 각각 연결된, 전극과 표준 전해 용액을 수용하는 제1 및 제2 레저버를 포함하는 적어도 하나의 전극 시스템을 포함하는 마이크로칩.
- 제1항에 있어서, 상기 제1 레저버와 수용된 전극과 상기 제2 레저버에 수용된 전극이 임피던스 분석기에 연결된 마이크로칩.
- 제2항에 있어서, 상기 제1 레저버에 수용된 표준 전해 용액에 존재하는 음이온이, 상기 마이크로채널을 가로질러 제1 폴리전해질 염다리로부터 제2 폴리전해질 염다리로 이동하며, 상기 음이온의 이동은 상기 마이크로 채널을 따라 유동하는 시료에 의해 간섭을 받고, 이것은 임피던스 변화를 야기하며, 상기 임피던스 분석기는 상기 임피던스의 변화를 검출하는 마이크로칩.
- 제3항에 있어서, 상기 임피던스 변화의 피크 진폭이 마이크로채널을 통해 이동하는 시료의 종류에 의존하는 마이크로칩.
- 제1항에 있어서, 마이크로칩 내에 상기 전극시스템이 일정한 거리를 두고 2개 존재하며, 두 전극 시스템 사이의 이격 거리와 첫 번째 전극시스템에서 발생한 임피던스의 변화와 두 번째 전극 시스템에서 발생한 임피던스의 변화의 시간차에 의해 시료의 이동 속도를 측정하는 마이크로칩.
- 제1항에 있어서, 상기 전극이 금속/금속염 전극인 마이크로칩.
- 제1항에 있어서, 상기 전극이 Ag/AgCl 전극인 마이크로칩.
- 제1항에 있어서, 상기 폴리전해질 염다리가 광중합에 의해 형성된 마이크로칩.
- 제1항에 있어서, 상기 폴리전해질 염다리가 폴리-디알릴디메틸 암모늄 클로라이드 염다리인 마이크로칩.
- 제1항에 있어서,a) 2개 이상의 세포를 함유하는 시료 용액을 도입하는 입구,b) 상기 입구로부터 도입된 시료 용액의 이동통로를 제공하는 마이크로 채널,c) 상기 마이크로 채널을 통과한 시료의 두개 이상의 출구,d) 상기 마이크로 채널에 서로 대향하게 연결된 한쌍의 제1 및 제2 폴리전해질 염다리와, 상기 제1 및 제2 폴리전해질 염다리에 각각 연결된, 전극과 표준 전해 용액을 수용하는 제1 및 제2 레저버를 포함하는 첫 번째 전극 시스템,e) 상기 마이크로 채널에 서로 대향하게 연결된 한쌍의 제3 및 제4 폴리전해질 염다리와, 상기 제3 및 제4 폴리전해질 염다리에 각각 연결된, 전극과 표준 전해 용액을 수용하는 제3 및 제4 레저버를 포함하는 두 번째 전극 시스템,f) 시료 용액의 이동방향을 전환시키는 수단을 구비하는 마이크로칩.
- 제10항에 있어서, 상기 출구가 2개 존재하며, 제1 출구는 상기 마이크로채널의 일직선상에 존재하며, 제2 출구는 마이크로채널의 일직선상으로부터 이탈된 곳에 위치하고, 시료 용액에 포함된 세포들 중에서 관심있는 세포의 경우, 상기 시료 용액의 이동방향을 전환시키는 수단에 의해 시료 용액의 이동방향이 변경되어 상기 세포가 제2 출구로 수집되고, 관심없는 세포의 경우 시료 용액의 이동방향을 전환시키는 수단이 미작동되어 상기 세포가 제1 출구로 이동하는 마이크로칩.
- 마이크로칩을 사용하여 세포 계수를 수행하는 방법에 있어서, 상기 마이크로칩이 제1항에 따른 마이크로칩인 것을 특징으로 하는 세포 계수 방법.
- 마이크로칩을 사용하여 세포의 빌로시메트리를 수행하는 방법에 있어서, 상기 마이크로칩이 제1항에 따른 마이크로칩인 것을 특징으로 하는 빌로시메트리 방법.
- 마이크로칩을 사용하여 세포의 분리 수집을 수행하는 방법에 있어서, 상기 마이크로칩이 제1항에 따른 마이크로칩인 것을 특징으로 하는 세포의 분리수집 방법.
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KR1020040097332A KR100581356B1 (ko) | 2004-11-25 | 2004-11-25 | 싸이토메트리, 빌로시메트리 및 세포 분류에 사용하기위한 폴리전해질 염다리를 사용하는 마이크로칩 |
PCT/KR2005/003988 WO2006057522A1 (en) | 2004-11-25 | 2005-11-25 | Microchip for use in cytometry, velocimetry and cell sorting using polyelectrolytic salt bridges |
US11/791,648 US20090104689A1 (en) | 2004-11-25 | 2005-11-25 | Microchip For Use In Cytometry, Velocimetry And Cell Sorting Using Polyelectrolytic Salt Bridges |
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KR1020040097332A KR100581356B1 (ko) | 2004-11-25 | 2004-11-25 | 싸이토메트리, 빌로시메트리 및 세포 분류에 사용하기위한 폴리전해질 염다리를 사용하는 마이크로칩 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100700713B1 (ko) | 2006-02-27 | 2007-03-28 | 한국표준과학연구원 | 신규한 폴리전해질 참조전극을 포함하는 소형화된 전기화학시스템 및 이것의 박층 전기분석으로의 응용 |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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KR100700713B1 (ko) | 2006-02-27 | 2007-03-28 | 한국표준과학연구원 | 신규한 폴리전해질 참조전극을 포함하는 소형화된 전기화학시스템 및 이것의 박층 전기분석으로의 응용 |
KR100807852B1 (ko) | 2007-02-08 | 2008-02-26 | 한국표준과학연구원 | 폴리전해질 염다리를 갖는 전기천공용 마이크로유동칩 |
KR100814477B1 (ko) | 2007-02-08 | 2008-03-17 | 주식회사 노마디엔 | 폴리전해질 염다리를 갖는 마이크로유동칩 상에서의 저전압전기천공법 |
WO2008150835A1 (en) * | 2007-05-31 | 2008-12-11 | Hemosense, Inc. | Particle fraction determination of a sample |
WO2010088506A2 (en) * | 2009-01-31 | 2010-08-05 | Purdue Research Foundation | A nanofluidic channel with embedded transverse nanoelectrodes and method of fabrication for same |
WO2010088506A3 (en) * | 2009-01-31 | 2011-02-03 | Purdue Research Foundation | A nanofluidic channel with embedded transverse nanoelectrodes and method of fabrication for same |
CN114272964B (zh) * | 2013-06-25 | 2024-04-23 | 华盛顿大学商业中心 | 样品体积的自数字化 |
KR101568903B1 (ko) | 2013-09-16 | 2015-11-12 | 서울대학교산학협력단 | 미세입자 계측 장치 및 그 방법 |
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