KR100561008B1 - Deodorizer having anti-bacterial activity - Google Patents

Abstract

The present invention relates to a deodorant with antimicrobial activity, comprising a mixture consisting of N-ethyl-N-soya morpholinium ethosulfate and citric acid. Deodorant of the present invention exhibits excellent deodorizing effect and antimicrobial activity, it can be effectively used for removing odor.

N-ethyl-N-soya morpholinium ethosulfate, citric acid, deodorizing effect, antibacterial activity

Description

Deodorizer Having Anti-bacterial Activity

The present invention relates to a deodorant having an antimicrobial activity. More specifically, the present invention relates to a deodorant having antimicrobial activity, comprising a mixture consisting of N-ethyl-N-soya morpholinium ethosulfate and citric acid.

As the development of civilization accelerates industrialization, urbanization and population concentration, environmental pollution, destruction of ecosystems and the generation of large amounts of garbage cause many problems. In particular, various bad smells such as food waste, livestock wastewater, and manure are causing serious problems.

In general, odor refers to a smell that irritating gaseous substances such as hydrogen sulfide, mercaptans, amines, ammonia, etc. stimulates the sense of smell of the person and causes discomfort or aversion. As a countermeasure against such odors, a ventilation dilution method that diffuses into the air by a hood or a duct or the like, a gastrointestinal method using a fragrance which disperses a component having a strong odor, and passes through a cleaner containing charcoal or cleaning liquid Absorption method that absorbs and removes materials, condensation method that condenses odorous substances using a cooler, combustion oxidation method that burns odorous substances directly into a flame at 600 ~ 800 ℃, and catalysts to oxidize odorous substances using catalysts such as platinum Oxidation methods, O ₃ , K, Mn and chemical oxidation method of chemically oxidizing odorous substances with compounds such as NaOCl, ClO ₂ and biological deodorization method using microorganisms are used. Among the above-mentioned methods, gout dilution method or gastrointestinal use method of medicine has the advantage that it is the least expensive, but it is not a fundamental solution because it does not remove the causative agent of odor. Absorption method, coagulation method, combustion oxidation method, catalytic oxidation method And chemical oxidation method decomposes the cause of odor and can be easily used even if the cause of the odor is a small amount, but in order to apply to the area where a large amount of odor causing substances such as food waste treatment plant, incinerator The disadvantage is that it takes a huge cost. On the other hand, the biological deodorization method has the advantage of excellent deodorizing effect and low installation cost and maintenance cost even when installed in the area where the odor causing substance is generated on a large scale, but it is only possible if the odor causing substance is above a certain scale. Therefore, depending on the type, scale, and location of the odor causing substance, an appropriate deodorizing method is selectively applied.

In particular, when removing odors generated in apartments or offices, it is difficult to apply biological deodorization methods or unpleasant by-products such as combustion oxidation methods that can be applied when there are odor causing substances above a certain scale, and absorption of odor removal efficiency is very low. Method, catalytic oxidation method using expensive catalyst, condensation method requiring a certain amount of cooler, chemical oxidation method using compound to be applied in large quantities is difficult to use. It is true.

However, as mentioned above, the ventilation dilution method and the use of medicine camouflage method are not a fundamental solution, and the method of applying other deodorization methods to a small place such as an apartment or an office has been steadily studied. Research into low-cost catalysts, compounds with excellent odor removal efficiency, etc. is being actively conducted, but there are no results.

Therefore, there is a constant need to develop a method that can effectively remove odors in a small place.

Accordingly, the present inventors have made diligent efforts to develop a method capable of effectively removing odor in a small place, and as a result, N-ethyl-N-soya morpholinium ethosulfate and citric acid It was confirmed that the composition comprising a not only can remove the odor with high efficiency, but also exhibit an antimicrobial activity to inhibit or sterilize the growth of microorganisms, to complete the present invention.

After all, the main object of the present invention is to provide an antibacterial deodorant comprising N-ethyl-N-soya morpholinium ethosulfate and citric acid.

Deodorant having an antimicrobial activity of the present invention includes a mixture of N-ethyl-N-soya morpholinium ethosulfate and citric acid 1: 1 to 1: 4 (w / w). The deodorizing agent having an antimicrobial activity of the present invention is used after dilution in a solvent such as water and lower alcohol, and the mixing ratio of the electrical composition and the solvent is not particularly limited and is appropriately diluted according to the purpose of use.

The present inventors searched for a safe compound that can be applied to the chemical oxidation method in order to develop a method that can effectively remove the odor in a small place, the search for a compound that meets the following criteria: Solubility in water High but low level of solubility in low alcohol or acetone, no color in water dissolved state, stable to pH change in dissolved state, most odor in home or office Phosphorus Shows activity to remove ammonia gas.

As a result of searching for a compound that satisfies the above criteria, the present inventors paid attention to N-ethyl-N-soya morpholinium etosulphate. N-ethyl-N-soya morpholinium ethosulfate is a compound in which the R group of alkyl-N-ethyl morpholinium ethosulfate is substituted with a soya alkyl group extracted from soya, an Indian soybean, It can be dissolved in water, lower alcohol and acetone. When dissolved in water, it does not show any color. It is stable against changes in pH in the dissolved state, and has an effect of removing ammonia gas.

However, since the removal efficiency of ammonia gas is low, a study was conducted to search for an auxiliary component that can improve the removal efficiency of ammonia gas. As a result, a mixture of N-ethyl-N-soya morpholinium ethosulfate and citric acid is used. In this case, it was confirmed that the removal efficiency of the ammonia gas can be significantly improved.

In addition, it was confirmed that the mixture of N-ethyl-N-soya morpholinium etosulphate and citric acid showed the activity of inhibiting the growth of microorganisms such as bacteria. At this time, the mixing ratio of N-ethyl-N-soya morpholinium ethosulfate and citric acid is not particularly limited, but N-ethyl-N-soya morpholinium ethosulfate and citric acid are 1: 1 to 1: 4 (w / When using the mixture consisting of w), it was confirmed that the excellent deodorizing effect and antibacterial effect.

Deodorant of the present invention exhibits excellent deodorizing effect and antimicrobial activity, it can be effectively used for removing odor.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Example 1 Measurement of Ammonia Gas Removal Efficiency of N-ethyl-N-Soya Morphonium Ethosulfate

300 g of pig manure is placed in a 1 m x 1 m x 1 m square sealed glass container, and left at 30 ° C. for 2 hours to induce the generation of ammonia gas, and then the concentration of ammonia gas contained in the air (C ₀ ) is 0.67. Boric acid trap device containing M boric acid solution (boric acid trap) and was measured through hydrochloric acid titration using 0.7N HCl. Then spray spray 50 ml of N, ethyl, N, soya morpholinium ethosulfate (Uniqema Asia Pacific Sdn. Bhd., Malaysia) at 0, 50, 100, 150, 200, 250, 300 and 350 µg / ml And, after leaving for 30 minutes at 30 ℃, the air in the closed glass container was discharged, the concentration of ammonia gas contained in the discharged air was measured in the same manner as described above (C _t ). Then, according to the following equation, the removal rate of ammonia gas was calculated and compared (see Table 1).

% Clearance = [(C ₀ -C _t ) / C ₀ ] × 100

Where

C ₀ is the concentration of ammonia gas measured prior to treatment with N-ethyl-N-soya morpholinium etosulphate; And,

C _t is the concentration of ammonia gas measured after treatment with N-ethyl-N-soya morpholinium ethosulfate.

Changes in Ammonia Gas Removal Rate of N-ethyl-N-Soya Morphonium Ethosulfate According to Treatment Concentration Treatment concentration of N-ethyl-N-soya morpholinium etosulphate (µg / ml) % Removal rate of ammonia gas 0 0 50 0 100 15 150 20 200 22 250 23 300 25 350 26

As shown in Table 1, the removal rate of ammonia gas was only 26% even when sprayed with N-ethyl-N-soya morpholinium ethosulfate at a concentration of 350 µg / ml in a space of 1 m 3 generated with ammonia gas. , N-ethyl-N-soya morpholinium etosulphate was found to have a very low level of ammonia gas removal efficiency.

Example 2 Screening of Auxiliary Components of N-Ethyl-N-Soya Morphonium Ethosulfate

As shown in Example 1, since N-ethyl-N-soya morpholinium etosulphate exhibited very low ammonia gas removal efficiency, an auxiliary component for improving ammonia gas removal efficiency for various organic and inorganic acids Searched.

First, 300 g of pig manure is placed in a 1 m x 1 m x 1 m square hermetically sealed glass container, and then left at 30 ° C. for 2 hours to induce the generation of ammonia gas. It was measured by the method.

Next, 50 ml of an aqueous solution containing 6.5 mg of N-ethyl-N-soya morpholinium ethosulfate and 0.1 mmol of lactic acid, fumaric acid, malic acid, citric acid, tartaric acid, picric acid, acetic acid, hydrochloric acid, and sulfuric acid were prepared, These were spray-sprayed in the airtight container, and left for 30 minutes at 30 ° C., then the air in the airtight glass container was discharged, and the concentration of ammonia contained in the discharged air was measured by the method of Example 1, followed by electric The removal rate of ammonia gas was calculated by the method of Example 1, and it was compared. In this case, only 50 ml of an aqueous solution in which 6.5 mg of N-ethyl-N-soya morpholinium ethosulfate was dissolved was sprayed and sprayed (see Table 2).

Changes in Ammonia Gas Removal Rate of N-ethyl-N-Soya Morphonium Ethosulfate and Various Acids with Various Acids mountain % Removal rate of ammonia gas Control 26 Lactic acid 30 Fumal 29 Malic acid 31 Citric acid 57 Tartaric acid 32 Piclinic acid 28 Acetic acid 29 Hydrochloric acid 27 Sulfuric acid 26

As shown in Table 2 above, even when most organic and inorganic acids were mixed with N-ethyl-N-soya morpholinium ethosulfate, the removal rate of ammonia gas could not be improved, but the removal rate of ammonia gas when citric acid was mixed It can be seen that this is significantly improved.

Example 3 Determination of the Mixing Ratio of N-ethyl-N-Soya Morphonium Ethosulfate and Citric Acid

As shown in Example 2, it was found that the mixture consisting of N-ethyl-N-soya morpholinium ethosulfate and citric acid effectively removed ammonia gas, and thus, the mixing ratio of them to effectively remove ammonia gas was determined. Was intended.

First, 300 g of pig manure is placed in a 1 m x 1 m x 1 m square hermetically sealed glass container, and then left at 30 ° C. for 2 hours to induce the generation of ammonia gas. It was measured by the method.

Then, respectively, 10mg: 1mg, 10mg: 2mg, 10mg: 5mg, 10mg: 10mg, 10mg: 20mg, 10mg: 30mg, 10mg: 40mg, 10mg: 50mg, 10mg: 60mg, 10mg: 70mg, 10mg: 80mg, 10mg: 90mg And 10 mg: 100 mg of N-ethyl-N-soya morpholinium ethosulfate and 50 ml of an aqueous solution of citric acid were prepared, which were spray-sprayed in an electric sealed container, and left at 30 ° C. for 30 minutes, and then sealed. The air in the glass container was discharged, and the concentration of ammonia contained in the discharged air was measured by the method of Example 1, and then the removal rate of ammonia gas was calculated using the method of Example 1, and compared. At this time, only 50 ml of 100 mg of citric acid aqueous solution was spray-sprayed as a negative control group, and only 50 ml of 100 mg of N-ethyl-N-soya morpholinium ethosulfate aqueous solution was sprayed as a positive control group (see Table 3).

Changes in Ammonia Gas Removal Rate with Mixing Ratio of N-ethyl-N-Soya Morphonium Ethosulfate and Citric Acid Mixing ratio (w / w) of N-ethyl-N-soya morpholinium etosulphate and citric acid % Removal rate of ammonia gas Positive control group 26 Negative Control 2 1: 0.1 38 1: 0.2 40 1: 0.5 45 1: 1 68 1: 2 72 1: 3 76 1: 4 81 1: 5 81 1: 6 81 1: 7 81 1: 8 81 1: 9 81 1:10 81

As shown in Table 3 above, when N-ethyl-N-soya morpholinium ethosulfate and citric acid are mixed at a ratio of 1: 1 (w / w) or more, the removal efficiency of ammonia gas is rapidly increased, and 1: 4 ( In the case of mixing more than w / w) it was confirmed that the removal efficiency of the ammonia gas is no longer increased.

Therefore, in order to effectively remove ammonia gas, it was found that it is preferable to use a mixture of N-ethyl-N-soya morpholinium ethosulfate and citric acid in a range of 1: 1 to 1: 4 (w / w). .

Example 4 Effect of a Composition Comprising N-Ethyl-N-Soya Morphonium Ethosulfate and Citric Acid on the Growth of Bacteria

It was confirmed how the mixture of N-ethyl-N-soya morpholinium etosulphate and citric acid, which were determined in Example 3, in the range of 1: 1 to 1: 4 (w / w), affected the growth of bacteria.

That is, 1 g of manure was diluted in 10 ml of LB liquid medium, and 500 µl was aliquoted, and 1 ml of LB liquid medium (control) and LB liquid medium 1 in which 10 mg of N-ethyl-N-soya morpholinium etosulphate was dissolved. ㎖ (Experimental group 1), 1 ml of LB liquid medium (experimental group 2) in which 10 mg of N-ethyl-N-soya morpholinium ethosulfate and 10 mg mol of citric acid were dissolved (experimental group 2), 10 mg of N-ethyl-N-soya morpholinium ethosulfate and citric acid 1 ml LB liquid medium in which 20 mg mol was dissolved (experimental group 3), 1 ml LB liquid medium in which 10 mg of N-ethyl-N-soya morpholinium ethosulfate and 40 mg of citric acid were dissolved (experimental group 4), and LB liquid in which 70 mg of citric acid was dissolved After mixing with 1 ml of medium (Experimental Group 5), the absorbance of each experimental group was measured at 600 nm. Subsequently, each experimental group was incubated at 30 ° C. for 24 hours, and then the absorbance was measured at 600 nm at 6 hour intervals, divided by the absorbance measured before the culture, and the change in bacterial cell number was compared (see Table 4).

Changes in bacterial cell count (%) following N-ethyl-N-soya morpholinium ethosulfate alone or in combination with citric acid Experimental group Elapsed time 6 hours 12 hours 18 hours 24 hours Control 135 241 417 833 Experimental group 1 123 209 361 644 Experiment group 2 100 110 121 133 Experiment group 3 92 85 80 78 Experimental Group 4 100 105 113 126 Experimental group 5 101 134 240 467

As shown in Table 4 above, when N-ethyl-N-soya morpholinium ethosulfate and citric acid were treated alone (Experimental Groups 1 and 5), the number of bacteria cells increased in a similar manner to the control group, but N-ethyl- When mixed with N-soya morpholinium ethosulfate and citric acid (experimental groups 2 to 4), it was found that the proliferation of bacteria was significantly suppressed (experimental groups 2 and 4) or decreased by death (experimental group 3).

Therefore, it was confirmed that the mixture of N-ethyl-N-soya morpholinium etosulphate and citric acid of 1: 1 to 1: 4 (w / w) of the present invention also exhibits antimicrobial activity that inhibits or sterilizes the growth of bacteria. Could.

As described and demonstrated in detail above, it provides a deodorant having an antimicrobial activity, including N-ethyl-N-soya morpholinium etosulphate and citric acid. Deodorant of the present invention exhibits excellent deodorizing effect and antimicrobial activity, it can be effectively used for removing odor.

Claims (2)

N-ethyl-N-soya morpholinium ethosulfate and an antibacterial deodorant comprising a mixture consisting of citric acid 1: 1 to 1: 4 (w / w) . The method of claim 1, Characterized in that it is used diluted with a solvent which is water or lower alcohol Deodorant with antimicrobial activity.