KR100549617B1 - The method of mixture of adsorbent and antibiotic material for foodstuffs packing film - Google Patents

The method of mixture of adsorbent and antibiotic material for foodstuffs packing film Download PDF

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KR100549617B1
KR100549617B1 KR1020020033158A KR20020033158A KR100549617B1 KR 100549617 B1 KR100549617 B1 KR 100549617B1 KR 1020020033158 A KR1020020033158 A KR 1020020033158A KR 20020033158 A KR20020033158 A KR 20020033158A KR 100549617 B1 KR100549617 B1 KR 100549617B1
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antimicrobial
film
silica
mixing
food
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KR20020054301A (en
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김현수
구본윤
이인선
유대식
성림식
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학교법인 계명기독학원
구본윤
김현수
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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K17/00—Safety valves; Equalising valves, e.g. pressure relief valves
    • F16K17/02—Safety valves; Equalising valves, e.g. pressure relief valves opening on surplus pressure on one side; closing on insufficient pressure on one side
    • F16K17/04—Safety valves; Equalising valves, e.g. pressure relief valves opening on surplus pressure on one side; closing on insufficient pressure on one side spring-loaded
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K27/00—Construction of housing; Use of materials therefor
    • F16K27/02—Construction of housing; Use of materials therefor of lift valves
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
    • F16K43/00—Auxiliary closure means in valves, which in case of repair, e.g. rewashering, of the valve, can take over the function of the normal closure means; Devices for temporary replacement of parts of valves for the same purpose

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  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)

Abstract

본 발명은 식품포장용 필름소재에 항균 원료의 혼합조성방법에 관한 것이며 투명색상을 유지하면서 식품의 보존에 유효하고 내열성이 우수한 천연항균물질을 흡착시킨 실리카를 필름소재에 혼합하여 필름의 제조시 항균물질이 열에 안정성을 부여하고 항균효과로 식품의 신선도 유지에 효과적인 식품포장용 필름 소재에 항균원료의 혼합조성방법을 제공함에 목적이 있다.The present invention relates to a method of mixing and mixing antimicrobial raw materials in food packaging film materials, which is effective for the preservation of foods while maintaining a transparent color, and is mixed with a film material containing silica adsorbed natural antimicrobial material having excellent heat resistance. It is an object of the present invention to provide a method of mixing and mixing antimicrobial raw materials in food packaging film material which gives stability to the heat and is effective for maintaining the freshness of food by antimicrobial effect.

본 발명은 토양의 미생물이 생산하는 항균성물질을 추출하여 실리카에 흡착시키고 동 실리카를 필름소재에 혼합하여 조성하는 방법이다.The present invention is a method of extracting the antimicrobial material produced by the microorganisms in the soil and adsorbed onto the silica and mixing the silica into a film material.

이와 같은 본 발명은 항균성 물질은 실리카에서 흡착이 이루어지고 항균성 물질이 흡착된 실리카는 무색으로 필름소재와의 혼합시에 필름의 투명도 유지에 장애 발생이 없고 필름이 제조되는 열에서도 안정되고 식품 포장시에는 항균성과 신선도 유지에 탁월한 효과를 가져올 수 있는 매우 유용한 발명이다.In the present invention, the antimicrobial material is adsorbed on silica, and the silica adsorbed on the antimicrobial material is colorless, so that the film is not disturbed in maintaining transparency of the film when mixed with the film material, and is stable even in the heat in which the film is produced and in food packaging. It is a very useful invention that can bring excellent effects in maintaining antibacterial and freshness.

식품포장, 미생물, 항균물질, 변패, 신선도 유지Food packaging, microorganisms, antibacterial substances, deterioration, freshness

Description

식품포장용 필름소재에 항균원료의 혼합조성방법{ The method of mixture of adsorbent and antibiotic material for foodstuffs packing film }The method of mixture of adsorbent and antibiotic material for foodstuffs packing film}

도1은 본 발명의 제조 공정의 블럭도1 is a block diagram of a manufacturing process of the present invention.

본 발명은 식품포장용 필름소재에 항균 원료의 혼합 조성방법에 관한 것이며 미생물이 생산하는 항균 원료를 실리카에 흡착시키고 이를 식품포장 필름소재에 혼합하여 변질과 부패를 억제하고 저장성을 높일 수 있는 식품포장용 필름을 제조할 수 있도록 한 것이다.The present invention relates to a mixed composition method of antimicrobial raw materials in a food packaging film material, and to adsorb the antimicrobial raw materials produced by microorganisms to silica and mixed with the food packaging film material to suppress alteration and decay, and to increase storage properties It is to be able to manufacture.

종래에는 청국장, 정육, 가공육, 선어, 염장식품등 각종의 식품은 선도유지와 장기보존을 위한 포장수단과 포장자재를 옛부터 수없이 연구되어 왔고 흡수, 가스조절, 습도조절, 항균물질등의 다양한 기능성 재료를 포장자재의 원료에 혼합하여 포장자재가 제조되고 이용되어 왔다.Conventionally, various foods such as Cheonggukjang, meat, processed meat, fresh fish, salted food have been studied for a long time and packing materials and packing materials for preservation of freshness and long-term preservation. Packaging materials have been manufactured and used by mixing functional materials with the raw materials of packaging materials.

또 신선도 유지를 위한 미생물 생육의 억제에 사용되는 방법으로는 저온 유지와 항균성 포장자재의 사용이 보편화되고 있다.In addition, as a method used for suppressing microbial growth for maintaining freshness, the use of low temperature maintenance and antimicrobial packaging materials is becoming common.

또한 식품에 있어 미생물의 생육은 1차적으로 표면에서 일어나고 생육 억제 를 위하여 항미생물제를 직접 포장필름에 첨가하여 식품의 표면접촉 또는 발산에 의해 생육을 억제하는 방법이 연구개발되고 있고, In addition, the growth of microorganisms in food occurs primarily on the surface, and in order to suppress growth, a method of inhibiting growth by surface contact or divergence of food by adding an antimicrobial agent directly to a packaging film has been researched and developed.

동 항균제는 식품으로 분류되어 식품첨가물로 허가되어 있는 합성품 또는 천연물로 한정하고 있고 현재까지 알려지고 있는 항균제는 은(Ag)의 항균효과를 이용하여 나트륨(Na) 이온을 은으로 치환 합성시킨 제오라이트(Zeolite)를 필름에 첨가하여 은이온이 식품속으로 서서히 방출하는 효과를 얻는 필름과, 항진균성 항생물질인 이마잘릴(Imazalil)을 폴리에틸렌필름(Low densilty polyethylene)의 소재에 첨가하여 과일, 채소, 치즈의 포장에서 곰팡이균의 생육억제 효과를 얻는 필름, 항균제인 벤노밀(Benomyl), 니신(Nisin), 유기산인 프로피오네이트(Propionate), 벤조에이트(Benzoate), 솔비트(Sorbate), 와사비 추출물, 키토산 등을 포장재료에 혼합 사용되고 있으나, 항균제는 경구독성의 정도에 따라 사용을 제한하는 국가도 있다. The antimicrobial agent is classified as a food and is limited to a synthetic or natural product that is approved as a food additive. The antimicrobial agent known to date is a zeolite that is synthesized by replacing sodium (Na) ions with silver using the antibacterial effect of silver (Ag). Zeolite) is added to the film to slowly release silver ions into food, and the antifungal antibiotic Imazalil is added to the material of low densilty polyethylene to make fruits, vegetables, and cheese. Film to obtain the growth inhibitory effect of the fungus in the packaging of, antimicrobial bennomyl (Benomyl), nisin (Nisin), organic acid propionate (propionate), benzoate (Sorbate), wasabi extract, Although chitosan is used in packaging materials, antimicrobial agents are restricted in some countries depending on the degree of oral toxicity.

또한 김등에 은의 항균력을 이용한 은을 입힌 세라믹 분말의 항균효과가 입증되어 항균세라믹을 첨가한 폴리에틸렌필름, 대황, 황련등 식물의 항균물질을 첨가한 폴리에틸렌필름, 김등의 합성 세라믹 분말에 항균성 물질을 흡착하여 조성한 항균제가 첨가된 폴리에틸렌필름이 알려지고 있다.In addition, the antimicrobial effect of silver coated ceramic powder using silver antibacterial activity has been proved. Polyethylene film to which the antimicrobial agent is added is known.

이들의 각 항균제가 함유된 식품포장용 소재는 안전성, 필름제조시 내열성, 식품 접촉시 용해성, 항균의 효율, 항균범위, 색상의 제한여부에 대한 검증이 이루어지지 못하고 있는 폐단이 있다.Food packaging materials containing these antimicrobial agents have been tested for safety, heat resistance during film production, solubility in contact with food, efficiency of antibacterial, antimicrobial range, and color limitations.

이에 본 발명은 투명색상을 유지하면서 식품 보존에 유효하고, 내열성이 우수한 천연 항균물질을 실리카에서 흡착하고 이를 필름소재에 혼합하여 필름 제조시 항균물질이 열에 안정성을 부여하고, 항균효과와 식품의 신선도 유지에 효과적인 식품포장용 필름의 소재에 항균원료의 혼합조성방법을 제공함에 목적이 있다.  Accordingly, the present invention is effective for food preservation while maintaining a transparent color, and absorbs natural antimicrobial material having excellent heat resistance in silica and mixes it with a film material to give stability to the antimicrobial material during film production, antibacterial effect and freshness of food An object of the present invention is to provide an antimicrobial raw material mixed composition method for food packaging films effective for fat and oil.

본 발명은 미생물이 생산하는 항균성 물질을 추출하여 실리카에 중량비로 1:99로 투여하여 실리카에서 흡착하고, 항균성물질이 흡착된 실리카와 필름소재는 0.5:99.5의 중량비율로 혼합 조성하는 방법이다.The present invention is a method of extracting the antimicrobial material produced by microorganisms and adsorbing the silica in a weight ratio of 1:99 and adsorbing the silica, and mixing the silica and the film material with the antimicrobial material adsorbed at a weight ratio of 0.5: 99.5.

이와 같은 본 발명에서 항균성 물질은 토양에서 분리하고 메탄올 자화방선균 M0-16 전배양균을 3% 되게 하여 방선균 생육배지인 ISP No.2 배지에서 접종하여 36~48시간을 배양한 다음 이를 3ℓ의 항균성 물질 생산배지인 M(Methanol)배지(K2HPO4 0.3%, NH4NO3 0.3% MgSO47H2O 0.02%, NaCl 0.1%, MeOH 1%, PH 7.0)에 6% 되게 접종하여 28℃, 40rpm에서 0.5ℓ/min 의 에어레이션(aeration)의 조건하에서 2일간 배양하고 배양액을 2배량의 에칠아세테이트(ethylacetate)를 이용하여 미생물에서 생산한 항균성 물질을 추출하고 에칠아세테이트 추출액에 무수황산나트륨(Na2S04)을 덩어리가 그 이상 형성되지 않도록 첨가하여 에칠아세테이트 추출액중에 함유하는 수분을 흡착 제거 후 진공농축한다.
이때 무수황산나트륨(Na2S04)의 첨가량은 수분함유량이 많으면 많아지게 된다.
또 본 발명에서 진공농축한 추출물은 정량하여 10g당 실리카(SiO2) 500g의 비율로 흡착되었고, 메탄올로 추출물을 용해하고 실리카를 첨가후 메탄올 실리카 용액이 600㎖가 되도록 조제하였다.In the present invention, the antimicrobial material was separated from the soil and inoculated in the ISP No. 2 medium, which is an actinomycete growth medium, to make 3% methanol magnetizing actinomycetes M0-16 precultured cultures, followed by incubation for 36 to 48 hours, followed by 3 l of antimicrobial activity. M (Methanol) medium (M 2 ) of material production medium (K 2 HPO 4 0.3%, NH 4 NO 3 0.3% MgSO 4 7H 2 O 0.02%, NaCl 0.1%, MeOH 1%, PH 7.0) was inoculated 6% at 28 ℃ , Incubated for 2 days under aeration of 0.5 L / min at 40 rpm, and extracted the antimicrobial substance produced by microorganisms using twice the amount of ethylacetate, and the anhydrous sodium sulfate (Na 2 S0 4 ) is added to prevent the formation of lumps, and the water contained in the ethyl acetate extract is removed by adsorption and concentrated in vacuo.
At this time, the amount of anhydrous sodium sulfate (Na 2 SO 4 ) is increased when the water content is large.
In addition, the vacuum concentrated extract in the present invention was quantified and adsorbed at a rate of 500 g of silica (SiO 2) per 10 g, and the extract was dissolved in methanol and prepared so that the methanol silica solution became 600 ml after addition of silica.

또 이와 같이 추출된 항균성 물질은 실리카에 흡착시키게 되는데 실리카에 항균성 물질을 투여시켜 실리카에 항균성 물질이 흡착되도록 하고, 항균성 물질이 흡착된 실리카를 필름소재에 혼합하므로서 항균물질이 혼합된 필름소재를 조성할 수 있게 된다.
본 발명에 사용하는 흡착제인 실리카는 내열성 천연 항균성 물질을 흡착하여 고온에서 내열성을 유지시킬수 있고, 항균성 물질의 용해에 사용하는 용매(메탄올)와의 균질한 혼합성, 필름제조시에는 용매의 휘발성이 요구되므로 실리카(SiO2)의 비율이 높고 수용성인 경우에는 친수성 용매인 메탄올에 혼합이 잘 되어 항균성 물질의 흡착이 용이하므로 수용성실리카담체를 사용하였으며 이는 친수성 물질이다.
상기 본 발명에서 사용하는 실리카 담체는 Degass사 제조, 상품명: Aerosil 이고 실리카(SiO2) 순도는 99.8%이상인 Hydrophilic Aerosil ＃300를 선택사용하였다.In addition, the extracted antimicrobial material is adsorbed on the silica, and the antimicrobial material is adsorbed on the silica to adsorb the antimicrobial material on the silica, and the antimicrobial adsorbed silica is mixed on the film material to form a film material mixed with the antimicrobial material. You can do it.
Silica, an adsorbent used in the present invention, can adsorb heat resistant natural antimicrobial substances to maintain heat resistance at high temperatures, homogeneous mixing with a solvent (methanol) used for dissolving the antimicrobial substances, and volatility of the solvent is required during film production. Therefore, when the ratio of silica (SiO2) is high and water-soluble, water-soluble silica carriers are used because they are easily mixed with methanol, which is a hydrophilic solvent, to facilitate the adsorption of antimicrobial substances.
The silica carrier used in the present invention was manufactured by Degass, trade name: Aerosil, and silica (SiO 2) purity of 99.8% or more was selected Hydrophilic Aerosil ＃ 300.

이와 같은 본 발명을 구체적인 실시예에 따라 그 혼합조성방법을 설명하면 다음과 같다.Referring to the mixing composition method according to the present invention as described above as follows.

[제1공정](항균성물질의 배양 및 추출공정)[Step 1] (Cultivation and Extraction Process of Antibacterial Substances)

토양에서 분리한 메탄올 자화방선균주(MO-16)를 ISP No.2 배지에 3% 되도록 접종하여 36~48시간을 배양하고 3ℓ의 M배지에 6%가 되도록 접종하여 28℃ 40rpm에서 0.5ℓ/min의 에어레이션(aeration)의 조건하에서 2일간 배양하고 그 배양액을 2배량의 에칠아세테이트(ethylacetate)를 이용하여 미생물이 생산한 항균성 물질을 추출하고 이를 황산나트륨(Na2SO4)으로 건조하여 진공농축한다.Methanol magnetizing radiation strain (MO-16) isolated from soil was inoculated to 3% in ISP No. 2 medium, incubated for 36 ~ 48 hours, and inoculated to 6% in 3L M medium, 0.5l / at 28 ℃ 40rpm. Incubate for 2 days under the condition of aeration of min, extract the antimicrobial substance produced by microorganisms using 2 times the amount of ethylacetate, dry it with sodium sulfate (Na 2 SO 4 ), and concentrate in vacuo. .

[제2공정](실리카에 항균물질의 흡착공정)[Step 2] (Adsorption of Antibacterial Substances on Silica)

제1공정의 항균성 물질 10g을 메탄올 500ml에 용해하여 실리카 0.5kg에 섞어 항균성 물질을 흡착시킨다(전부 600ml정도).10 g of the antimicrobial substance of the first step is dissolved in 500 ml of methanol and mixed with 0.5 kg of silica to adsorb the antimicrobial substance (around 600 ml).

[제3공정](필름소재에 항균물질의 혼합공정)[3rd process] (mixing process of antibacterial substance on film material)

필름소재에 제2공정의 실리카를 혼합하게 되는데 중량비율로 필름소재 99.5kg에 제2공정의 실리카 0.5kg을 혼합하여 필름소재를 조성한다.Silica of the second process is mixed to the film material, and 99.5 kg of the film material is mixed with 0.5 kg of the silica of the second process in a weight ratio to form a film material.

[제4공정](필름제조공정)[Step 4] (Film Production Process)

제3공정의 필름소재를 이용하여 필름제조기에서 필름을 제조한다.The film is manufactured in a film maker using the film material of the third step.

[제5공정](제품화 공정)[Step 5] (Production Process)

제4공정으로 제조되는 필름을 규격으로 권취하거나 재단하여 식품포장용 필름으로 제품화 한다.The film produced by the 4th process is wound up or cut to a standard and commercialized into a film for food packaging.

이와 같은 본 발명에서 항균성 물질은 필름의 제조조건인 150℃ 에서 30초 동안 가열된 상태에서 항균의 기능에서는 별다른 변화없이 안정된 상태를 유지하였고, 또 실리카는 흰색이고 항균성 물질이 흡착되어 필름소재와 혼합하였으나 필름의 색상이 투명상태로 유지되므로서 식품의 포장상태를 육안으로 확인이 가능하고 실제 항균효과로서는 그램(Gram) 양성 세균에는 배양액 및 에칠아세테이트(ethylacetate) 추출물 모두 강한 항균활성을 나타내었고, 그램 음성 세균에는 살모넬라 티피(Salmonaella Typhi) KCTC 2424를 제외한 모든균에 항균활성을 나타내었고, 에칠아세테이트 추출물의 경우에는 살모넬라 티피, KCRC2424 및 사카로마이세스세레비지아(Saccharomyces cerevisiae)에도 항균활성을 나타내었고 또 간암세포(0.004ug/mℓ), 대장암세포(0.002ug/mℓ), 쥐백혈병세포(0.006ug/mℓ)에서 미량의 항암효과를 보이기는 하나 필름에는 극미량이 용출되므로서 안정성에는 별다른 문제가 없는 것으로 확인되었다.In the present invention, the antimicrobial material was maintained in a stable state without any change in the antibacterial function while heated at 150 ° C. for 30 seconds, which is a manufacturing condition of the film, and the silica was white and the antimicrobial material was adsorbed and mixed with the film material. However, since the color of the film is maintained in a transparent state, it is possible to visually check the packaging state of the food. As a practical antimicrobial effect, both the culture solution and the ethylacetate extract showed strong antimicrobial activity to Gram-positive bacteria. Negative bacteria showed antimicrobial activity against all bacteria except Salmonaella Typhi KCTC 2424, and acetylacetate extract also showed antimicrobial activity against Salmonella typhi, KCRC2424 and Saccharomyces cerevisiae. In addition, liver cancer cells (0.004ug / mℓ), colon cancer cells (0.002ug / ml), rat leukemia cells (0.0 06ug / mℓ) showed a small amount of anti-cancer effect, but it was confirmed that there is no problem in stability as the trace amount is eluted from the film.

또 본 발명은 미생물이 생산하는 천연항균성 물질을 추출하고 이를 실리카(silica)에 흡착시켜 필름소재에서 혼합하여 식품포장용 필름을 제조하게 되므로서 포장되는 식품을 미생물에 의한 변패를 억제하게 되어 저장성을 높일 수 있게 되고, 또 동항균성 물질이 흡착된 실리카를 121℃에서 5분간을 열처리 한 바 항균력과 내열성에 변화가 없는 식품포장용 필름 항균원료를 얻을수 있게 되고, 또 필름에 투명상태를 유지하며 식품의 포장상태를 육안으로 확인이 용이하여 식품포장용 필름으로 사용이 용이하게 되는 것이다.In addition, the present invention extracts the natural antimicrobial material produced by the microorganism and adsorbs it to silica (silica) to mix in a film material to produce a film for food packaging to suppress the deterioration caused by the microorganism in the packaged food to improve shelf life After heat-treating silica adsorbed by the antimicrobial substance at 121 ° C for 5 minutes, it is possible to obtain a film antimicrobial material for food packaging which does not change antimicrobial activity and heat resistance, and maintains a transparent state on the film to package food. It is easy to check the state with the naked eye is easy to use as a food packaging film.

아래의 도표들은 본 발명에 의한 실험예를 표시한 것이다. The following chart shows an experimental example according to the present invention.

[표1]열처리에 의한 본 발명의 잔존활성Table 1 Residual Activity of the Invention by Heat Treatment

종 류             Kinds 열처리(분)  Heat treatment (minutes)                                          none 0.5 1 2 3 5 none 0.5 1 2 3 5 Gram(+) 박테리아 바실러스 테모글루코시더스(Bacillus themoglucosidus) 스타필료코쿠스 아우레우스(Staphylococus aureus) 스트렙토코쿠스 에퀴(Streptococcus equii) 스트렙토코쿠스 주에피더미쿠스 (Streptococcus zooepidermicus) Gram(-) 박테리아 에쉐리치아 코리(Esherichia coli) 슈도모나스 에루지노사(Pseudomonas aeruginosa) 세라티아 마스센스(Serratia marcescens) 펀지(Fungi) 캔디다 알비캔스(Candida albicans) 아스페길러스 푸미가투스(Aspergillus fumigatus) Gram (+) bacteria Bacillus themoglucosidus Staphylococus aureus Streptococcus equii Streptococcus zooepidermicus Gram (-) bacteria Esherichia coli Pseudomonas aeruginosa Serratia marcescens Punjab Candida albicans Aspergillus fumigatus 저해구역(φ, mm) 15 15 15 14 15 14 12 13 13 12 12 13 10 10 10 9 9 9 13 13 13 13 13 13 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 11 11 - - - - - - + - - - - -          Inhibition zone (φ, mm)                                               15 15 15 14 15 14 12 13 13 12 12 13 10 10 10 9 9 9 13 13 13 13 13 13                                                                                                                                           12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 11 11                                                                                              ------+-----

[표2] 필름에 의한 분쇄된 돼지고기의 보존효과[Table 2] Preservation effect of ground pork by film

배양시간(일)  Incubation time (days) 분쇄된 돼지고기 Ground pork                                          일반필름    General film 이건발명   Invention 1 실내온도 3 6 8                    1 Room temperature 3 6 8 5 ×102 6 ×104 9 ×102 1 ×102      5 × 102   6 × 104   9 × 102   1 × 102 1 ×102 3 ×104 2 ×107 8 ×108 1 × 102   3 × 104  2 × 107   8 × 108  1 3 4℃ 6 8                     1 3 4 ℃ 6 8 2 ×102 3 ×102 4 ×102 3 ×103      2 × 102   3 × 102   4 × 102   3 × 103 3 ×102 3 ×102 2 ×102 1 ×103     3 × 102   3 × 102  2 × 102  1 × 103

[표3] 세균배양기 접시 수단에 의한 필름의 항균 테스트[Table 3] Antimicrobial test of film by bacterial culture plate

샘플Sample 일반필름 이건발명      General Film This invention 0.2g 0.4g 0.2g 0.4g       0.2g 0.4g 0.2g 0.4g 이 콜리(E.coli)E. coli 59 42 43 33        59 42 43 33

[표4] 여러음식물의 저장에서 본 발명의 저장상태[Table 4] Storage state of the present invention in the storage of various foods

FoodFood 저장상태Storage status 포장안함 일반필름 이건발명  Unpacked General Film This invention 만다린 오렌지(Mandarine orage) 아가리쿠스 비스포루스 (Agaricus bisporus) 작은 토마토(Small tomato) 풋고추(unripe Red pepper)  Mandarin orage Agaricus bisporus Small tomato Unripe red pepper 양호 부패(100%) 부패(30%) 건조 부패(60%) 양호 부패(20%) 건조 부패(100%) 부패(100%) 양호 건조 부패(50%) 부패(20%)   Good Corruption (100%) Corruption (30%) Dry Corruption (60%) Good Corruption (20%) Dry Corruption (100%) Corruption (100%) Good Dry Corruption (50%) Corruption (20%)

(포장된 음식은 실내온도에서 30일동안 저장하였다.(Packed foods were stored at room temperature for 30 days.

저장상태는 시각적인 관찰에 의해 '양호', 부패율'로 표현되었다.The storage state was expressed as 'good', corruption rate by visual observation.

양호: 부패없는 탁월한 보존Good: excellent preservation without corruption

% : 부패율 )%: Corruption Rate

따라서 이상과 같은 본 발명은 토양의 미생물이 생산하는 천연 항균성 물질을 실리카에 흡착시키고 이를 식품제조용 필름소재에 혼합 조성하여 식품포장용 필름을 제조하여 동 필름으로 식품을 포장하게 되면 식품은 미생물에 의한 변패를 예방하여 저장성을 높일 수 있게 되는 산업상 매우 유용한 발명이다. Therefore, the present invention as described above is adsorbed on the natural antimicrobial substances produced by microorganisms in the soil and mixed with them to the food manufacturing film material to produce a food packaging film to package the food with the same film, the food is changed by microorganisms It is a very useful invention in the industry to prevent storage to increase storage.

Claims (1)

미생물이 생산하는 항균성 물질을 실리카에서 흡착하고 항균성 물질이 흡착된 실리카를 필름 소재에 혼합조성함을 특징으로 한 식품포장용 필름소재에 항균 원료의 혼합조성 방법Method of mixing and mixing antimicrobial raw materials in food packaging film material characterized by adsorbing antimicrobial material produced by microorganisms on silica and mixing and adsorbing silica adsorbed on antimicrobial material to film material
KR1020020033158A 2002-06-14 2002-06-14 The method of mixture of adsorbent and antibiotic material for foodstuffs packing film KR100549617B1 (en)

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