KR100537480B1 - Manufacturing Method of Tarakjuk Enhancing Content of Enzyme Resistant Starch - Google Patents

Manufacturing Method of Tarakjuk Enhancing Content of Enzyme Resistant Starch Download PDF

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KR100537480B1
KR100537480B1 KR10-2004-0002590A KR20040002590A KR100537480B1 KR 100537480 B1 KR100537480 B1 KR 100537480B1 KR 20040002590 A KR20040002590 A KR 20040002590A KR 100537480 B1 KR100537480 B1 KR 100537480B1
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rice flour
minutes
rice
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water
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KR20050074720A (en
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이귀주
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학교법인고려중앙학원
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C1/00Concentration, evaporation or drying
    • A23C1/14Concentration, evaporation or drying combined with other treatment
    • A23C1/16Concentration, evaporation or drying combined with other treatment using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L23/00Soups; Sauces; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Cereal-Derived Products (AREA)

Abstract

본 발명은 멥쌀 1kg을 수세 후 가수하여 실온에서 2시간 침지하고 30분간 체에 받쳐 물기를 제거한 후, 방앗간에서 물과 소금을 넣지 않고 빻은 다음 두 손으로 가루를 비벼서 고르게 하고, 습식제분한 멥쌀가루 300g을 네모난 팬 (28×35×4cm)에 고르게 펴서 열풍건조기에서 40oC에서 5시간 건조한 후, 쌀가루가 덩어리지지 않고 균일하게 되도록 체(355㎛)를 내린 다음 -20oC에서 냉동보관함으로써 볶음에 사용하기 위한 쌀가루를 제조하고; 쌀가루 40g을 원형의 팬(지름 21cm×높이 4cm)에 고르게 볶아질 수 있도록 5mm 이하의 두께로 얇게 편 후, 열풍건조기에서 185oC로 25분간 볶은 다음 1시간 실온에 방치하여 냉각시키고, 냉각된 볶음 쌀가루를 체(180∼355㎛)에 내려 진공 비닐백에 넣은 후 -20oC에서 냉동보관함으로써 볶음 쌀가루를 제조하고; 볶음 쌀가루 15g을 400mL 비커에 넣고 우유 150g을 가하여 잘 저어 분산시킨 다음 5분간 실온에 방치하고, 쌀가루-우유 혼합물이 담긴 비커를 항온 수조(water bath)에 담근 후 크램프로 고정하고, 비커 상부에 랩 교반기(Lab stirrer(MS-3030®, Tops, Korea))를 장치하고 임펠러(impeller)를 고정한 후, 저어주면서 죽의 내부온도가 50-56.5℃가 되도록 항온 수조(water bath)의 온도를 조절하여 2시간-5시간 동안 가열하고, 완료 5분 전에 0.5g의 소금을 넣고 다시 가열함으로써 타락죽을 제조하는 것으로 이루어지는, 효소저항전분 함량을 증진시키는 타락죽의 제조방법에 관한 것이다.In the present invention, 1kg of non-glutinous rice is washed with water, immersed in water for 2 hours at room temperature, and then drained by a sieve for 30 minutes, and then ground in a mill without adding water and salt. Spread 300g evenly in a square pan (28 × 35 × 4cm), dry for 5 hours at 40 o C in a hot air dryer, lower the sieve (355 μm) so that rice powder does not clump and store frozen at -20 o C. Thereby preparing rice flour for use in roasting; 40 g of rice flour is thinly sliced to a thickness of 5 mm or less so that it can be roasted evenly in a circular pan (21 cm in diameter x 4 cm in height), and then roasted in a hot air dryer at 185 o C for 25 minutes and left to stand at room temperature for 1 hour to cool. To stir fried rice flour in a sieve (180 ~ 355 ㎛) put in a vacuum plastic bag and frozen at -20 ° C to prepare fried rice flour; Add 15 g of fried rice flour to a 400 mL beaker, add 150 g of milk, stir well, disperse, and leave at room temperature for 5 minutes, soak the beaker containing the rice flour-milk mixture in a water bath, fix with a cramp, and wrap on top of the beaker After installing a stirrer (Lab stirrer (MS-3030 ® , Tops, Korea)) and fixing the impeller, the temperature of the water bath is controlled so that the temperature of the porridge is 50-56.5 ℃ while stirring. The present invention relates to a method for producing perilla, which comprises enzymatic resistance starch content, which is prepared by heating for 2-5 hours and adding 0.5 g of salt 5 minutes before completion and heating again.

Description

효소저항전분의 함량을 증진시키는 타락죽의 제조방법 {Manufacturing Method of Tarakjuk Enhancing Content of Enzyme Resistant Starch}Manufacturing Method of Fallen Porridge Enhancing Enzyme-Resistant Starch Content {Manufacturing Method of Tarakjuk Enhancing Content of Enzyme Resistant Starch}

본 발명은 효소저항전분의 함량을 증가시키는 타락죽의 제조방법에 관한 것으로서, 구체적으로는 타락죽의 원료가 되는 쌀의 볶음조건, 호화조건 및 이를 이용한 타락죽의 제조 조건을 최적화하여 효소저항전분(Enzyme resistant starch: RS)의 함량을 최대화하고, 이에 따라 칼로리 기여도를 최소화한, 타락죽의 제조방법에 관한 것이다.The present invention relates to a method for producing decay porridge to increase the content of enzyme-resistant starch, and specifically, enzyme resistance starch (Enzyme resistant) by optimizing the roasting conditions, gelatinization conditions, and preparation conditions of decay porridge of the rice, which is the raw material of the decay porridge. starch: RS) to maximize the content, thereby minimizing the contribution of calorie, relates to a method of producing perilla.

쌀은 한국인의 필수 에너지원으로 사용되는 기본식품으로서, 전분 이외에도 단백질과 소량의 지방 및 불포화지방산을 함유하고 있으며 기타 무기질, 비타민도 소량씩 함유하고 있다. 단백질 함량은 많지 않으나 에너지원으로서 매끼 다량의 쌀을 섭취하기 때문에 한국인의 경우 식사로부터 상당량의 단백질을 얻게 되는데, 섭취하는 식품중에서 열량의 47%, 단백질의 30%를 쌀로부터 얻고 있다.Rice is a basic food used as an essential energy source for Koreans. It contains not only starch but also protein, small amount of fat and unsaturated fatty acid, and small amount of other minerals and vitamins. Although the protein content is not high, a large amount of rice is consumed as an energy source, so Koreans obtain a considerable amount of protein from their meals. 47% of the calories and 30% of the protein is taken from the foods they eat.

그러나, 최근 우리나라 사람들의 국민소득과 생활수준이 급격히 향상되고 외식문화가 발전하면서 쌀을 이용한 식품을 기피하는 경향이 갈수록 확산되고 있으며, 이에 따라 쌀의 1인당 연간 소비량은 2000년도에 93.6kg에서 2002년도에는 87kg으로 감소하는 추세에 있고, 이렇게 소비량이 현저하게 감소함으로써 쌀값이 하락하고 논농사를 주 수입원으로 하는 농가가 경제적인 빈곤에 허덕이고 있는 실정이다.However, as the national income and living standards of Koreans have sharply improved and the food culture has been developed recently, the tendency to avoid foods using rice is spreading. As a result, the annual consumption of rice per capita in 2002 was 93.6kg in 2002. In the year, it is decreasing to 87kg, and the consumption is remarkably reduced, causing the price of rice to fall, and farmers whose main income is rice paddy are suffering from economic poverty.

그러나, 쌀의 구체적인 소비 행태를 살펴보면, 종래 주식인 취반(炊飯)용이 대부분이고, 술, 떡, 고추장 등을 만드는데 일부분이 사용되던 것이 최근에는 쌀국수, 쌀과자, 쌀음료 등 쌀을 주재로 하는 가공식품의 개발이 가속화되고 있으며, 심지어는 쌀비누 등을 필두로 하여 식용이 아닌 공산품에까지 쌀을 이용하고자 하는 시도가 다양하게 전개되고 있는 추세인데, 쌀 소비량 감소에 의한 농가수입 증대이라는 측면에서 바람직한 일이라고 할 수 있다.However, when looking at the specific consumption behavior of rice, most of the conventional stocks for cooking (炊 飯), and part of it was used to make sake, rice cake, red pepper paste, etc. Recently, processing such as rice noodles, rice cakes, rice drinks, etc. The development of food is accelerating, and there are various attempts to use rice even for non-edible industrial products, even rice soap, etc., which is desirable in terms of increasing farm income by reducing rice consumption. It can be said.

이러한 다양한 쌀의 용도개발은 전통적인 쌀농사를 보전하여 국가경제적 기반을 공고히 하고 식량무기화에 대비하려는 정부의 제도적, 경제적 지원에 힘입은 바도 큰데, 본 발명 역시 이와 같은 쌀 소비량의 증가를 목적으로 하는 가공식품 개발 시도중 하나라고 볼 수 있다.The development of the various uses of rice is largely supported by the government's institutional and economic support for conserving traditional rice farming, solidifying the national economic foundation and preparing for food weaponization, and the present invention is also processed for the purpose of increasing rice consumption. One of the food development attempts.

쌀의 가공은 쌀이 함유하고 있는 전분 및 단백질의 영양가와 생체이용율에 영향을 주는 것으로 알려지고 있는데, 그 중에서도 쌀 가공시 가장 흔하게 이용되는 공정이라고 할 수 있는 열처리, 호화 및 부분호화는 전분의 효소에 의한 가수분해를 촉진시키는 것이 일반적이나, 지방산 및 지질의 존재 하에서는 효소에 의한 전분의 소화율을 감소시키는 것으로 보고되고 있다.The processing of rice is known to affect the nutritional value and bioavailability of starch and protein in rice. Among them, heat treatment, gelatinization and partial dehydration, which are the most commonly used processes in rice processing, are enzymes of starch. It is common to promote hydrolysis by but it has been reported to reduce the digestibility of starch by enzymes in the presence of fatty acids and lipids.

보리에서 연구된 바에 따르면, 호화온도 이하에서 전분은 지질과 현저한 상호작용을 일으키지 않으나, 호화온도 부근에서의 가열처리는 효소저항성인 아밀로즈-지질 복합체(amylose-lipid 복합체)의 형성을 촉진시키며 보리전분의 소화흡수율을 감소시키는데, 이는 아밀로즈-지질 복합체가 아밀로즈(amylose)의 용출을 방해하며, 물속에서 가열된 전분입자의 팽윤을 저해하고, 전분의 수분결합능력을 감소시키기 때문인 것으로 밝혀졌다.Studies on barley have shown that starch does not interact significantly with lipids below the gelatinization temperature, but heat treatment near the gelatinization temperature promotes the formation of the enzyme-resistant amylose-lipid complex. It was found that starch digestion absorption was reduced because amylose-lipid complexes inhibited the elution of amylose, inhibited swelling of heated starch particles in water, and reduced starch's water-binding capacity. .

그동안 전분은 소장에서 완전히 소화되어 흡수되는 탄수화물로 생각되어져 왔으나, 근래 소화효소에 분해 되지 않고 소장을 지나 대장에서 장내 미생물군에 의해 발효되는 일련의 전분분획이 존재한다는 것이 알려졌으며 이 분획들은 통칭 효소저항전분 (enzyme resistant starch, 이하 RS)으로 명명되었는데, 이러한 RS의 함량은 식품의 성분조성, 가공방법, 아밀로즈(amylose) 함량 등에 따라 다르게 나타나는 것으로 보고되고 있다.Starch has been thought to be a carbohydrate that is completely digested and absorbed in the small intestine, but recently it has been known that there is a series of starch fractions that are not degraded by digestive enzymes but pass through the small intestine and fermented by the intestinal microflora. It is named as resistance starch (RS), and it is reported that the content of RS is different depending on the composition of food, processing method, amylose content, etc.

RS의 효소에 대한 저항성은 거칠게 제분한 곡류에서처럼 물리적으로 포획된 전분입자(RS1), 부적절한 조리로 인한 호화되지 않은 전분입자(RS2), 또는 광범위한 수소결합으로 결정성이 증가된 노화된 아밀로즈(amylose) 분획(RS3) 등에 기인하는 것으로 알려져 있다.RS's resistance to enzymes can be achieved by physically trapped starch particles (RS1) as in roughly milled grains, ungelatinized starch particles due to improper cooking (RS2), or aged amylose with increased crystallinity due to extensive hydrogen bonding. amylose) fraction (RS3).

이와 같은 RS는 대장에서 발효되어 낙산염(butyrate)과 같은 단쇄지방산을 생성하고 이들 대사산물은 장의 pH를 감소시킴으로써 식이섬유와 같은 생리활성을 나타내는 것으로 알려지고 있는데, RS가 높은 식품은 대부분의 건강한 성인에게 이로운 것으로 생각되어지고 있기 때문에 RS 형성은 섬유질이 풍부한 건강 기능성식품을 만드는 새로운 도구가 되고 있다.These RSs are fermented in the large intestine to produce short-chain fatty acids, such as butyrate, and these metabolites are known to exhibit physiological activities such as dietary fiber by reducing the pH of the intestines. RS formation has become a new tool for making fiber-rich health functional foods, which are believed to be beneficial to adults.

이와 같은 RS의 특성은 영양학적으로 전분분해속도 및 소장에서의 흡수속도, 나아가 낮은 당지수와 관련되는데, RS에 대한 이러한 최근의 개념은 전분 함량에 비해 칼로리 기여가 적은 가공식품을 개발하는 데에 중요한 단서를 제공하고 있다.This characteristic of RS is nutritionally related to the rate of starch decomposition and absorption in the small intestine, and even to a low glycemic index. This recent concept of RS has been used to develop processed foods with less calorie contribution to starch content. It provides important clues.

한편, 타락죽은 물에 불린 쌀을 맷돌에 갈아서 절반쯤 끓이다가 유유를 섞어 다시 끓인 죽, 불린 쌀을 그늘에 말려 가루로 만들어 볶은 다음 다시 고운 가루로 만들어 심말(心末)을 물에 개어 끓이다가 우유를 넣고 다시 약한 불로 끓여낸 죽, 또는 멥쌀가루를 노르스름하게 볶아서 유유만으로 끓인 죽 등을 말하며, 달리는 낙죽 또는 유유죽이라 불리우는 한국의 전통 궁중음식으로서, 조리서에 따라서 그 제조방법이 조금씩 차이를 보이고 있는데, 멥쌀가루를 볶아서 우유와 함께 가열하는 제조방법의 경우, 멥쌀가루를 볶는 온도와 시간 그리고 우유와 함께 가열하는 온도와 시간에 따라서 완성되는 타락죽의 품질특성이 크게 달라지는 것으로 알려져 있다.Meanwhile, the decayed rice is milled in a millstone and boiled about half, then mixed with milk and boiled again.The dried rice is dried in a shade to make powder, then roasted, and then made into a fine powder. It is porridge boiled on low heat with milk or boiled non-glutinous rice powder and boiled with only yuyu. It is a traditional Korean court food called Nak-juk or Yu-yu-juk. In the manufacturing method of roasting non-glutinous rice flour and heating it with milk, it is known that the quality characteristics of the decayed porridge are greatly changed depending on the temperature and time of roasting non-glutinous rice flour and the temperature and time of heating it with milk.

이와 같은 타락죽은 그 맛과 영양면에서 가공식품으로서의 충분한 잠재력을 가지고 있는데, 상기한 RS의 특성을 이용하여 타락죽에서의 RS 함량을 높임으로써, 최근 각광받고 있는 건강식품으로써의 용도를 개발함으로써 쌀에 대한 새로운 용도를 하나 더 창출하고, 이에 따라 쌀의 이용을 제고할 수 있는 시도가 필요한 시점이라고 할 수 있다.Such decayed rice has the full potential as a processed food in terms of taste and nutrition. By using the properties of the above-mentioned RS to increase the RS content in the decayed rice, it has been developed for the use as a health food that has recently been in the spotlight. At this point, it is time to try to create another new use and to increase the use of rice.

본 발명은 상기한 바와 같은 종래기술의 문제점을 극복하고자 하는 것으로서, 그 목적은, 타락죽의 원료가 되는 쌀의 볶음 조건, 호화 조건 및 이를 이용한 죽의 제조 조건을 최적화하여 효소저항전분(Enzyme resistant starch: RS)의 함량을 최대화하고, 이에 따라 칼로리 기여도를 최소화함으로써 성인용 건강식품으로 사용될 수 있는, 타락죽의 제조방법을 제공하고자 하는 것이다.The present invention is to overcome the problems of the prior art as described above, the object of the enzymatic resistance starch (Enzyme resistant starch) by optimizing the roasting conditions, gelatinization conditions and production conditions of porridge using the same as the raw material of the decay porridge : To maximize the content of RS), thereby minimizing calorie contributions to provide a method of manufacturing perilla, which can be used as adult health food.

이를 위한 본 발명은, 멥쌀 1kg을 수세 후 가수하여 실온에서 2시간 침지하고 30분간 체에 받쳐 물기를 제거한 후, 방앗간에서 물과 소금을 넣지 않고 빻은 다음 두 손으로 가루를 비벼서 고르게 하고, 이렇게 습식제분한 멥쌀가루 300g을 네모난 팬(28×35×4cm)에 고르게 펴서 열풍건조기에서 40oC에서 5시간 건조한 후 쌀가루가 덩어리지지 않고 균일하게 되도록 체(355㎛)를 내린 다음 -20oC에서 냉동보관함으로써 볶음에 사용하기 위한 쌀가루를 제조하고; 냉동보관 쌀가루 40g을 원형의 팬(지름 21cm×높이 4cm)에 고르게 볶아질 수 있도록 5mm 이하의 두께로 얇게 편 후, 열풍건조기에서 185oC로 25분간 볶은 다음 1시간 실온에 방치하여 냉각시키고, 냉각된 볶음 쌀가루를 체(180∼355㎛)에 내려 진공 비닐백에 넣은 후 -20oC에서 냉동보관함으로써, 볶음 쌀가루를 제조하고; 냉동보관 볶음 쌀가루 15g을 400mL 비커에 넣고 우유 150g을 가하여 잘 저어 분산시킨 다음 5분간 실온에 방치하고, 쌀가루-우유 혼합물이 담긴 비커를 항온 수조(water bath)에 담근 후 크램프로 고정하고, 비커 상부에 랩 교반기(Lab stirrer(MS-3030®, Tops, Korea))를 장치하고 임펠러(impeller)를 고정한 후, 저어주면서 죽의 내부온도가 50-56.5℃가 되도록 항온 수조(water bath)의 온도를 조절하여 2시간-5시간 동안 가열하고, 완료 5분 전에 0.5g의 소금을 넣고 다시 가열함으로써 타락죽을 제조하는 것으로 이루어지는, 효소저항전분 함량을 증진시키는 타락죽의 제조방법을 제공하는 것으로 이루어진다.To this end, the present invention, 1kg of non-glutinous rice after washing with water, immersed in water for 2 hours at room temperature for 30 minutes to remove water, crushed in a mill without adding water and salt, and then rub the powder with both hands to make even, so wet Lower the body (355㎛) I square the milled rice flour 300g fan (28 × 35 × 4cm) evenly stretched out and dried for 5 hours at 40 o C in a hot air dryer to ensure uniform without being a mass of rice flour and then -20 o C Preparing frozen rice powder for use in roasting by freezing; After freezing, store 40g of rice flour in a circular pan (21cm in diameter x 4cm in height) and thinly slice it to a thickness of 5mm or less, fry in a hot air dryer at 185 o C for 25 minutes, and let it stand at room temperature for 1 hour to cool. The cooled fried rice flour was lowered in a sieve (180-355 µm), placed in a vacuum plastic bag, and stored frozen at -20 ° C. to prepare fried rice flour; Freeze-stored Stir-fried rice powder 15g into a 400mL beaker, add 150g of milk, stir well, disperse and leave at room temperature for 5 minutes, soak the beaker containing the rice flour-milk mixture in a water bath and fix it with a cramped top Install a stirrer (Lab stirrer (MS-3030 ® , Tops, Korea)) on the top, fix the impeller, and stir the temperature of the water bath so that the inside temperature of the porridge is 50-56.5 ℃ while stirring. It is made to provide a method for producing perilla, which is made of perilla, which is prepared by adjusting the heating for 2 hours to 5 hours and adding 0.5 g of salt 5 minutes before completion to heat perilla.

본 발명은 효소저항전분(Enzyme resistant starch: RS)의 함량을 증가시키는 타락죽의 제조방법에 관한 것으로서, 구체적으로는, 타락죽의 원료가 되는 쌀의 볶음 조건, 호화 조건 및 이를 이용한 죽의 제조 조건을 최적화하여 효소저항전분의 함량을 최대화하고 이에 따라 타락죽의 칼로리 기여도를 최소화함으로써, 성인용 건강식품으로 사용할 수 있는 타락죽의 제조방법에 관한 것이다.The present invention relates to a method for producing decay porridge to increase the content of enzyme resistant starch (RS), specifically, the roasting conditions, gelatinization conditions and conditions for producing porridge using the same of Optimizing the content of enzyme-resistant starch by optimizing and thereby minimizing the contribution of calorie of perilla, relates to a method for producing perilla, which can be used as an adult health food.

본 발명에서는, 효소저항전분의 함량을 최대화하는 타락죽의 제조방법을 제공하기 위하여, 여러 온도에서 볶음을 수행한 후 각 결과물의 효소저항전분의 함량을 측정함으로써 타락죽 제조시 사용되는 최적의 멥쌀가루의 볶음 온도를 시험하였고, 다시 여러 온도에서 호화를 수행한 후 각 결과물의 효소저항전분 함량을 측정함으로써 역시 적절한 호화 온도를 시험하였다.In the present invention, in order to provide a method for producing decay porridge to maximize the content of enzyme-resistant starch, after the roasting at various temperatures by measuring the content of the enzyme-resistant starch of each result of the optimum non-rice flour used in the preparation of The roasting temperature was tested, and again appropriate gelatinization temperature was tested by performing gelatinization at various temperatures and measuring the enzyme resistance starch content of each resultant.

또한, 각 가열조건에서 수득된 타락죽을 이용 in vitro 전분분해율, 시차주사열량기(DSC)에 의한 호화특성 등을 수행함으로써, 본 발명의 방법에 의해 제조된 타락죽이 단지 효소저항전분 함량이 높을 뿐만 아니라 성인용 건강식품으로서 충분한 조건을 갖추고 있다는 것을 증명하였다.In addition, by performing the in vitro starch decomposition rate, differential scanning calorimeter (DSC), and the like using the decay porridge obtained under each heating condition, the decay porridge prepared by the method of the present invention only has a high enzyme resistance starch content. Rather, it proved that they have sufficient conditions for adult health foods.

이하, 제조예 및 이를 이용한 시험예로서 본 발명을 더 구체적으로 설명하면 다음과 같다.Hereinafter, the present invention will be described in more detail as a production example and a test example using the same.

제조예 및 시험예에 사용한 멥쌀은 2001년 수확되어 농촌진흥청 작물시험장에서 도정한 일품벼(19% amylose)이며, 그밖에 우유(서울우유?)와 소금(정제염, 해표?)은 시중에서 구입하여 사용하였다.Uncooked rice used in the production and test examples was harvested in 2001 and was harvested at Rural Development Administration's Crop Experiment Station. The rice was 19% amylose. In addition, milk (Seoul Milk ? ) And salt (table salt, Haecheo ? ) Were purchased and used commercially. .

[제조예 1] 볶음 쌀가루의 제조Preparation Example 1 Preparation of Fried Rice Flour

최대 효소저항전분 함량을 가지는 멥쌀의 볶음 조건을 설정하기 위하여 하기와 같은 제조예를 수행하여 볶음 쌀가루를 제조하였다.In order to set the roasting conditions of non-glutinous rice having the maximum enzyme resistance starch content, the following preparation example was carried out to prepare fried rice flour.

먼저, 볶음에 사용하기 위한 쌀가루를 제조하기 위하여, 시험용 및 대조구용 멥쌀 1kg을 수세 후 가수하여 실온에서 2시간 침지하고 30분간 체에 받쳐 물기를 제거한 후, 방앗간에서 물과 소금을 넣지 않고 빻은 다음 두 손으로 가루를 비벼서 고르게 하였다. 다음에, 이렇게 습식제분한 쌀가루 300g을 네모난 팬(28×35×4cm)에 고르게 펴서 열풍건조기(조선과학기계제작소, 한국)를 이용 40℃에서 5시간 동안 건조시킨 쌀가루를 사용하였다.First, to prepare rice flour for use in stir-fries, 1 kg of non-glutinous rice for test and control was washed with water, immersed in water for 2 hours, immersed in a sieve for 30 minutes, dried, and then ground in a mill without adding water and salt. The powder was rubbed evenly with both hands. Next, 300 g of the wet milled rice flour was evenly spread on a square pan (28 × 35 × 4 cm), and rice flour was dried at 40 ° C. for 5 hours using a hot air dryer (Chosun Science Machinery Co., Ltd., Korea).

이렇게 건조된 시험용 및 대조구용 쌀가루는 덩어리지지 않고 균일하게 되도록 체(355㎛)를 내린 후 볶음시험 전 까지 -20oC에서 냉동 보관하였다.The dried rice powder for test and control was thus sieved (355 μm) so as not to be lumped and then stored frozen at −20 ° C. until the stir test.

다음에, 효소저항전분 함량을 증진시키는 최적의 볶음조건을 시험하기 위하여, 상기한 바와 같이 건조한 쌀가루중 시험용 쌀가루 각 40g을 원형의 팬(지름 21cm×높이 4cm)에 고르게 볶아질 수 있도록 5mm 이하의 두께로 얇게 편 후, 열풍건조기에서 145oC, 165oC 및 185oC로 각각 25분과 40분간 볶은 다음 1시간 실온에 방치하여 식히고, 이렇게 제조된 시험용 볶음 쌀가루는 체(180∼355㎛)에 내려 진공 비닐백에 넣은 후 시험에 이용되기 전까지 냉동 (-20oC) 보관하였다.Next, in order to test the optimum roasting conditions for enhancing the enzyme resistance starch content, as described above, each 40 g of the test rice powder in dry rice powder can be roasted evenly in a circular pan (21 cm diameter x 4 cm in height) of 5 mm or less. After thinly sliced, roasted for 25 minutes and 40 minutes at 145 o C, 165 o C, and 185 o C, respectively, in a hot air dryer, and allowed to cool at room temperature for 1 hour. The test fried rice flour thus prepared was sieved (180-355 μm). It was placed in a vacuum plastic bag and stored frozen (-20 o C) until used for testing.

[시험예 1][Test Example 1]

이렇게 제조된 볶음 쌀가루들을 이용하여 각각의 효소저항전분의 함량을 측정하였다.Using the prepared fried rice flour was measured the content of each enzyme resistance starch.

각 볶음 멥쌀가루의 효소저항전분 함량은 Goni 등의 방법(Goni, I., Garcia-Diz, E., Manas, E. and Saura-Calixto, F. Analysis of resistant starch: a method for foods and food products. Food Chem. 56: 445-449 (1996))을 이용하여 측정하였다.Enzyme-resistant starch content of each fried non-glutinous rice flour was determined by Goni et al. (Goni, I., Garcia-Diz, E., Manas, E. and Saura-Calixto, F. Analysis of resistant starch: a method for foods and food products Food Chem. 56: 445-449 (1996)).

먼저, 시료 100mg을 50mL 원심관(centrifuge tube)에 넣고 10mL의 0.02M HCl용액과 0.2mL 펩신용액(Sigma Chemical Company, P-7000, 871units/mg protein, 1g pepsin/10mL 0.02M HCl)을 넣은 다음 볼텍싱(vortexing)하고, 40℃의 진탕 항온 수조(shaking water bath)에서 60분간 진탕배양 하였다.First, 100 mg of sample is placed in a 50 mL centrifuge tube, followed by 10 mL of 0.02 M HCl solution and 0.2 mL pepsin solution (Sigma Chemical Company, P-7000, 871 units / mg protein, 1 g pepsin / 10 mL 0.02 M HCl). It was vortexed and shaken for 60 minutes in a shaking water bath at 40 ° C.

효소분해한 시료를 꺼내어 상온으로 식힌 후, 9mL의 0.1M 삼중 말레인산염 완충제(Tris-maleate buffer: pH 6.9, 4mM CaCl2 함유)와 1mL의 α-아밀라제(α-amylase) 용액(Sigma Chemical Co., A-3176, 40mg α-amylase/mL of Tris-maleate buffer)을 넣고 볼텍싱(vortexing)한 다음, 37℃의 진탕 항온 수조(shaking water bath)에서 16시간 동안 진탕배양 하였다.After enzymatic digestion, the sample was cooled to room temperature, and then 9 mL of 0.1M trimaleate buffer (pH 6.9, containing 4 mM CaCl 2 ) and 1 mL of α-amylase solution (Sigma Chemical Co., Ltd.). , A-3176, 40mg α-amylase / mL of Tris-maleate buffer) was added and vortexed, followed by incubation for 16 hours in a shaking water bath at 37 ° C.

16시간 후에 시료를 원심분리(15min, 3000g)한 후 상등액을 버리고, 잔류물(residue)에 10mL의 증류수를 넣어 씻은 다음에 다시 원심분리하고 상등액을 버린다.After 16 hours, the sample was centrifuged (15 min, 3000 g), and then the supernatant was discarded. 10 mL of distilled water was added to the residue, washed, and then centrifuged again and the supernatant was discarded.

잔류물(residue)에 3mL의 증류수를 넣어 시료를 적신 다음 3mL의 4M KOH 용액을 넣어 볼텍싱(vortexing)하고, 상온에서 30분간 진탕배양 하였다.3 mL of distilled water was added to the residue, and the sample was wetted. Then, 3 mL of 4M KOH solution was added to the solution and vortexed, followed by shaking culture at room temperature for 30 minutes.

5.5mL의 2M HCl과 3mL의 0.4M 소듐 아세테이트 완충제(sodium acetate buffer)를 넣은 다음 0.1mL의 아밀로글루코시다스(amyloglucosidase(Novozyme, AMG E))를 넣고 볼텍싱(vortexing)한 후, 60℃에서 45분간 진탕배양 하였다.Add 5.5 mL of 2M HCl and 3 mL of 0.4M sodium acetate buffer, add 0.1 mL of amyloglucosidase (Novozyme, AMG E), and vortex to 60 ° C. The culture was shaken for 45 minutes at.

분해가 끝난 후 원심분리하고 상등액을 50mL 용량플라스크에 모은다. 잔류물(residue)에 한번 이상 10mL의 증류수를 넣고(no vortexing) 씻은 다음 같은 방법으로 원심분리한 후, 상등액을 취해서 이전의 상등액과 합하여 50mL 용량플라스크에 합치고 증류수로 50mL로 정용(定容)하여 글루코스 농도측정에 사용하였다.After digestion, centrifuge and collect the supernatant in a 50 mL volumetric flask. Put 10 mL of distilled water at least once in the residue (no vortexing), wash, centrifuge in the same way, take the supernatant, combine with the previous supernatant, add 50 mL volumetric flask, and settle to 50 mL with distilled water. Used to measure glucose concentration.

상등액 0.1mL에 글루코즈 옥시다스-페록시다스 키트(glucose oxidase-peroxidase kit(GOD-POD kit, Novozyme))을 넣고 전체 용량이 3.2mL가 되게 하여 37℃의 항온 수조(water bath)에서 30분간 배양한 후, 분광 광도계(spectrophotometer)를 사용하여 500nm에서 흡광도를 측정함으로써(실제로는 글루코스 표준곡선을 만들고, 측정한 흡광도로부터 글루코스 함량을 계산) 글루코스 함량을 측정하였고, 글루코스 함량에 0.9를 곱하여 시료 내 효소저항전분의 함량을 구하였으며, 그 결과를 하기 표 1에 나타내었다.Glucose oxidase-peroxidase kit (GOD-POD kit, Novozyme) was added to 0.1 mL of the supernatant, and the total volume was 3.2 mL, followed by incubation for 30 minutes in a 37 ° C water bath. The glucose content was then measured by measuring the absorbance at 500 nm using a spectrophotometer (actually creating a glucose standard curve and calculating the glucose content from the measured absorbance), and multiplying the glucose content by 0.9 to the enzyme in the sample. The content of the resistance starch was obtained, and the results are shown in Table 1 below.

볶음 쌀의 효소저항전분 함량Enzyme Resistance Starch Content in Fried Rice 볶음온도(℃)Roasting temperature (℃) 볶음시간(분)Roasting time (minutes) RS함량(건물당 %)RS content (% per building) 대조구Control 16.20(0.83)16.20 (0.83) 145145 2525 17.47(1.44)17.47 (1.44) 4040 14.52(1.71)14.52 (1.71) 165165 2525 18.34(0.36)18.34 (0.36) 4040 16.31(1.71)16.31 (1.71) 185185 2525 18.93(1.15)18.93 (1.15) 4040 15.91(1.42)15.91 (1.42)

a. RS함량은 3반복 평균임a. RS content is 3 repetition average

b. 괄호안의 숫자는 p<0.05에서의 표준편차임.b. The numbers in parentheses are the standard deviation at p <0.05.

시험 결과, 대조구의 RS 함량은 건물당 16.2%이었으며, 볶음시간이 25분일 때에는 볶음온도가 증가함에 따라서 RS 함량이 증가하여 185℃에서 25분간 볶은 멥쌀가루가 18.9%로서 대조구보다 RS 함량이 16% 증가함으로써 가장 높았다.As a result, the RS content of the control was 16.2% per building, and when the roasting time was 25 minutes, the RS content increased with the increase of the roasting temperature, and 18.9% of the non-glutinous rice flour roasted at 185 ° C for 25 minutes was 16% higher than the control. Highest by increasing.

볶음시간이 40분으로 증가하면 각 볶음온도에서의 RS 함량은 대조구보다 감소하였으나 유의적인 차이를 나타내지 않았다.When the roasting time increased to 40 minutes, the RS content at each roasting temperature was lower than that of the control, but there was no significant difference.

일반적으로 쌀의 RS 함량은 낮은 것으로 보고되었는데, 본 시험에서 볶지 않은 대조구의 RS 함량이 16.2%로 높게 나타난 것은 대조구가 습식제분한 멥쌀가루를 40℃에서 5시간 열풍건조기로 건조시킨 쌀가루로서 수분의 존재 하에서 열처리를 받았기 때문인 것으로 추측되었으며, 또한, 본 시험에서 멥쌀가루를 40분 볶음시 각 볶음 온도에서 RS 함량이 대조구보다 감소한 것은 볶음과정 중 호정화(dextrinization) 과정이 시작되는 것에 기인하는 것으로 추측되었다.In general, the RS content of the rice was reported to be low. In this test, the RS content of the unroasted control was high as 16.2%. It was estimated that the heat treatment was performed in the presence of the present invention. Also, in the present test, the decrease of RS content at each roasting temperature when roasting non-glutinous rice powder for 40 minutes was attributed to the dextrinization process during the roasting process. It became.

[제조예 2] 타락죽의 제조Preparation Example 2 Preparation of Fallen Porridge

최대 효소저항전분 함량을 시험하기 위한 샘플로서의 타락죽을 제조하기 위하여, 하기와 같이 제조예를 수행하였다.In order to prepare the perilla as a sample for testing the maximum enzyme resistance starch content, the preparation example was carried out as follows.

먼저, 타락죽 제조가 가능한 가열온도 범위를 알아내기 위하여, 상기 시험예 1에서 효소저항전분 함량을 가장 크게 증진시키는 것으로 선정된 볶음 쌀가루, 즉 185℃에서 25분간 볶은 멥쌀가루를 이용하여 시차주사열량기(Differential Scanning Calorimetry: DSC, Seiko Instrument, DSC 6100, Chiba, Japan)로 하기와 같이 호화특성을 측정하였다.First, in order to find out the heating temperature range in which the perishables can be produced, a differential scanning calorimeter is made using stir-fried rice flour selected as the largest enhancement of the enzyme resistance starch content in Test Example 1, that is, non-glutinous rice flour roasted for 25 minutes at 185 ° C. Gelatinization properties were measured as follows (Differential Scanning Calorimetry: DSC, Seiko Instrument, DSC 6100, Chiba, Japan).

알루미늄팬(Seiko Instrument, Chiba, Japan) 위에 볶음 쌀가루 시료 2mg을 넣고 현탁액의 수분 함량이 80%가 되도록 증류수를 넣은 후 즉시 밀봉하여 실온에서 하룻밤 방치한 후, 시료를 10-170℃까지 5℃/분의 속도로 가열하였으며, 이로부터 얻은 시차주사열량기 서머그램(DSC thermogram) 상으로부터 호화개시온도(To), 최대호화온도(Tp), 호화종결온도(Tm) 및 엔탈피(ΔH)를 측정하였으며, 이때 인듐과 주석을 사용하여 온도와 엔탈피 값을 보정하여 그 결과를 하기 표 2에 나타내었다.Put 2mg of fried rice flour sample on an aluminum pan (Seiko Instrument, Chiba, Japan), add distilled water so that the water content of the suspension is 80%, seal it immediately, leave it at room temperature overnight, and then put the sample at 5 ℃ / to 10-170 ℃. It was heated at the rate of minutes, and from the obtained differential scanning calorimeter (DSC thermogram) on the gelatinization start temperature (To), maximum luxurious temperature (Tp), gelatinization end temperature (Tm) and enthalpy (ΔH) were measured. In this case, indium and tin were used to correct the temperature and enthalpy values, and the results are shown in Table 2 below.

185℃, 25분 볶음 쌀가루의 호화특성Luxury Characteristics of Stir-Fried Rice Flour at 185 ℃ for 25 Minutes ToTo TpTp TmTm ΔHΔH 49.57(0.21)49.57 (0.21) 56.50(0.30)56.50 (0.30) 65.73(0.57)65.73 (0.57) 11.70(0.12)11.70 (0.12)

a. 각 측정치는 3반복 평균임a. Each measurement is a three iteration average

b. 괄호안의 숫자는 p<0.05에서의 표준편차임.b. The numbers in parentheses are the standard deviation at p <0.05.

다음에, 상기와 같이 측정된 호화특성을 이용하여, 가열온도를 상기 호화최대온도(Tp)인 56.5℃를 중심으로, 하기 표 3에 나타낸 바와 같이, 50℃, 56.5℃, 64℃ 및 69℃로, 각각 가열시간을 달리하여 타락죽을 제조하였다.Next, using the gelatinization characteristics measured as described above, the heating temperature was centered at 56.5 ° C, which is the gelatinization maximum temperature (Tp), as shown in Table 3 below, at 50 ° C, 56.5 ° C, 64 ° C, and 69 ° C. Furnace was prepared by varying the heating time, respectively.

타락죽의 제조 온도 및 시간Manufacturing Temperature and Time of Fallen Rice 가열온도(℃)Heating temperature (℃) 가열시간(분)Heating time (minutes) 5050 240240 300300 56.556.5 120120 240240 6464 6060 120120 6969 3030 6060

각 가열온도에서의 가열시간은 예비시험을 통하여 타락죽이 형성되는 시간을 기반으로 설정하였으며, 그 구체적인 타락죽의 제조방법은 하기와 같다.The heating time at each heating temperature was set based on the time that the perilla was formed through a preliminary test, and the method for producing the perilla was as follows.

먼저, 볶음 쌀가루 15g을 400mL 비커에 넣고 우유 150g을 가하여 잘 저어서 분산시킨 다음 5분간 실온에 방치하고, 쌀가루-우유 혼합물이 담긴 비커를 항온 수조(water bath)에 담근 후 크램프로 고정하고, 비커 상부에 랩 교반기(Lab stirrer(MS-3030®, Tops, Korea))를 장치하고 임펠러(impeller)를 고정하여 저어주면서 죽의 내부온도가 각각 상기 표 3과 같이 되도록 항온 수조(water bath)의 온도를 조절하여 지정된 시간 동안 가열하였다. 완료 5분 전에 0.5g의 소금을 넣어 가열함으로써 타락죽을 완성하고, 실온에서 냉각 즉시 동결건조하여 체(180-355㎛)에 친 후 시험에 사용하였다.First, add 15 g of fried rice powder to a 400 mL beaker, add 150 g of milk, stir well, disperse, and leave at room temperature for 5 minutes, soak the beaker containing the rice flour-milk mixture in a water bath and fix it with a cramp, Install a stirrer (Lab stirrer (MS-3030 ® , Tops, Korea)) at the top and stir by fixing the impeller (impeller) while stirring the temperature of the water bath (water bath) so that the internal temperature of the pores as shown in Table 3, respectively Was adjusted to heat for the designated time. Five minutes prior to completion, 0.5 g of salt was added to complete the perilla, and immediately cooled at room temperature, lyophilized, sieved (180-355 μm), and used for testing.

[시험예 2] 타락죽 가열조건 설정[Test Example 2] Falling porridge heating conditions

효소저항전분의 함량을 가장 크게 증진시킬 수 있는 타락죽의 가열조건을 측정하기 위하여 하기와 같은 시험을 수행하였다.In order to determine the heating conditions of the perishable bamboo which can greatly enhance the content of enzyme resistance starch, the following test was carried out.

상기 표 3에 따른 각각의 가열조건에 의해 제조된 타락죽의 효소저항전분 함량을 하기와 같이 측정하였다.The enzyme resistance starch content of the perilla prepared by each heating condition according to Table 3 was measured as follows.

각 볶음 멥쌀가루의 효소저항전분 함량은 Goni 등(Goni, I., Garcia-Diz, E., Manas, E. and Saura-Calixto, F. Analysis of resistant starch: a method for foods and food products. Food Chem. 56: 445-449 (1996))의 방법을 이용하여 측정하였다.Enzyme-resistant starch content of each fried non-glutinous rice flour was determined by Goni et al. (Goni, I., Garcia-Diz, E., Manas, E. and Saura-Calixto, F. Analysis of resistant starch: a method for foods and food products.Food Chem. 56: 445-449 (1996)).

먼저, 시료 100mg을 50mL 원심관(centrifuge tube)에 넣고 10mL의 0.02M HCl용액과 0.2mL 펩신용액(Sigma Chemical Company, P-7000, 871units/mg protein, 1g pepsin/10mL 0.02M HCl)을 넣은 다음 볼텍싱(vortexing)하고, 40℃의 진탕 항온 수조(shaking water bath)에서 60분간 진탕배양 하였다.First, 100 mg of sample is placed in a 50 mL centrifuge tube, followed by 10 mL of 0.02 M HCl solution and 0.2 mL pepsin solution (Sigma Chemical Company, P-7000, 871 units / mg protein, 1 g pepsin / 10 mL 0.02 M HCl). It was vortexed and shaken for 60 minutes in a shaking water bath at 40 ° C.

효소분해한 시료를 꺼내어 상온으로 식힌 후, 9mL의 0.1M 삼중 말레인산염 완충제(Tris-maleate buffer(pH 6.9, 4mM CaCl2 함유))와 1mL의 α-아밀라제(α-amylase) 용액(Sigma Chemical Co., A-3176, 40mg α-amylase/mL of Tris-maleate buffer)을 넣고 볼텍싱(vortexing)한 다음, 37℃의 진탕 항온 수조(shaking water bath)에서 16시간 동안 진탕배양 하였다.The enzyme digested sample was taken out and cooled to room temperature, followed by 9 mL of 0.1M trimaleate buffer (containing Tris-maleate buffer (pH 6.9, 4 mM CaCl 2 )) and 1 mL of α-amylase solution (Sigma Chemical Co.). , A-3176, 40mg α-amylase / mL of Tris-maleate buffer) was added and vortexed, and then shaken for 16 hours in a shaking water bath at 37 ° C.

16시간 후에 시료를 원심분리(15min, 3000g)한 후 상등액을 버리고, 잔류물(residue)에 10mL의 증류수를 넣어 씻은 다음에 다시 원심분리하고 상등액을 버린다.After 16 hours, the sample was centrifuged (15 min, 3000 g), and then the supernatant was discarded. 10 mL of distilled water was added to the residue, washed, and then centrifuged again and the supernatant was discarded.

잔류물(residue)에 3mL의 증류수를 넣어 시료를 적신 다음 3mL의 4M KOH 용액을 넣어 볼텍싱(vortexing)하고, 상온에서 30분간 진탕배양 하였다.3 mL of distilled water was added to the residue, and the sample was wetted. Then, 3 mL of 4M KOH solution was added to the solution and vortexed, followed by shaking culture at room temperature for 30 minutes.

5.5mL의 2M HCl과 3mL의 0.4M 소듐 아세테이트 완충제(sodium acetate buffer)를 넣은 다음 0.1mL의 아밀로글루코시다스(amyloglucosidase(Novozyme, AMG E))를 넣고 볼텍싱(vortexing)한 후, 60℃에서 45분간 진탕배양 하였다.Add 5.5 mL of 2M HCl and 3 mL of 0.4M sodium acetate buffer, add 0.1 mL of amyloglucosidase (Novozyme, AMG E), and vortex to 60 ° C. The culture was shaken for 45 minutes at.

분해가 끝난 후 원심분리하고 상등액을 50mL 용량플라스크에 모은다.After digestion, centrifuge and collect the supernatant in a 50 mL volumetric flask.

잔류물(residue)에 한번 이상 10mL의 증류수를 넣고(no vortexing) 씻은 다음 같은 방법으로 원심분리한 후 상등액을 취해서 이전의 상등액과 합하여 50mL 용량플라스크에 합치고 증류수로 정용하여 글루코스 농도측정에 사용하였다.10 mL of distilled water (no vortexing) was added to the residue at least once, washed, and then centrifuged in the same manner. The supernatant was taken, combined with the previous supernatant, combined with a 50 mL volumetric flask, and used as a distilled water to measure glucose concentration.

상등액 0.1mL에 글루코스 옥시다스-페록시다스 키트(glucose oxidase-peroxidase kit(GOD-POD kit, Novozyme))를 넣고 전체 용량이 3.2mL가 되게 하여 37℃의 항온 수조(water bath)에서 30분간 배양한 후, 분광 광도계(spectrophotometer)를 사용하여 500nm에서 흡광도를 측정함으로써 글루코스함량을 측정하였고, 글루코스 함량에 0.9를 곱하여 시료 내 효소저항전분의 함량을 구하였으며, 그 결과를 하기 표 4에 나타내었다.Glucose oxidase-peroxidase kit (GOD-POD kit, Novozyme) was added to 0.1 mL of the supernatant, and the total volume was 3.2 mL, followed by incubation for 30 minutes in a 37 ° C water bath. After that, the glucose content was measured by measuring the absorbance at 500 nm using a spectrophotometer, and the content of the enzyme resistance starch in the sample was obtained by multiplying the glucose content by 0.9 and the results are shown in Table 4 below.

타락죽의 효소저항전분 함량Enzyme-resistant starch content of degenerated rice 가열온도(℃)Heating temperature (℃) 가열시간(분)Heating time (minutes) RS함량(건물당 %)RS content (% per building) 대조구Control 1010 5.42(0.49)5.42 (0.49) 5050 240300240300 10.16(2.14)9.42(2.14)10.16 (2.14) 9.42 (2.14) 56.556.5 120240120240 6.75(0.43)5.95(0.19)6.75 (0.43) 5.95 (0.19) 6464 6012060120 4.68(0.71)5.15(0.43)4.68 (0.71) 5.15 (0.43) 6969 30603060 3.43(0.92)4.22(1.03)3.43 (0.92) 4.22 (1.03)

a. RS함량은 3반복 평균임.a. RS content is 3 repeat average.

b. 괄호안의 숫자는 p<0.05에서의 표준편차임.b. The numbers in parentheses are the standard deviation at p <0.05.

시험 결과, 가열온도 50℃와 56.5℃에서 각각 4시간과 5시간 가열시 RS 함량은 10.16%와 9.42%로 나타났는데, 이는 타락죽을 재래식으로 10분간 끓여서 제조한 대조구의 RS 함량인 5.42%보다 2배 가량 높은 것이었다. 반면, 가열온도 64℃와 69.5℃에서 각각 2시간과 1시간 가열시 RS 함량은 5.15%와 4.22%로서, 대조구의 RS 함량보다도 더 낮은 것이었다.As a result, the RS content was 10.16% and 9.42% at 4 ℃ and 56.5 ℃ at 50 ℃ and 56.5 ℃, respectively, which is 2 times higher than that of 5.42% of the control prepared by boiling perilla for 10 minutes. It was about twice as high. On the other hand, the RS content was 5.15% and 4.22% when heated for 2 hours and 1 hour at 64 ° C and 69.5 ° C, respectively, which was lower than that of the control.

전체적으로 볼 때, 타락죽의 RS 함량은 가열온도가 50℃, 56.5℃, 대조구, 64℃, 69℃의 순서로 감소하는 경향을 나타냄으로써, 호화개시온도(To)를 넘는 범위에서 가능하면 온도가 낮게 가열하는 것이 효소저항전분 함량을 더 높이는 결과를 가져오는 것을 알 수 있었다.As a whole, the RS content of the decayed rice tends to decrease in the order of 50 ° C, 56.5 ° C, control, 64 ° C, and 69 ° C, so that the temperature is as low as possible in the range exceeding the initiation temperature (To). Heating was found to result in higher enzyme resistance starch content.

한편, 50℃와 56.5℃에서는 가열시간이 증가할수록 RS 함량은 감소하였으나 64℃와 69.5℃에서는 가열시간이 증가할수록 RS 함량은 증가하였는데, 이로써, 낮은 온도에서 가능하면 짧은 시간 동안 가열하는 것이 RS함량을 증진시킴을 알 수 있었다.On the other hand, at 50 ℃ and 56.5 ℃, the RS content decreased with increasing heating time, but at 64 ℃ and 69.5 ℃, the RS content increased with increasing heating time. It can be seen that it promotes.

결과적으로, 상기 시험예 1에서 선정된 185℃에서 25분간 볶은 멥쌀가루를 이용하여 최대의 효소저항전분 함량 증진효과를 가져올 수 있는 타락죽 제조 조건은 50℃-56.5℃에서 2시간-5시간 가열하는 것으로 정리할 수 있게 되었다.As a result, using the rice flour roasted for 25 minutes at 185 ℃ selected in Test Example 1 decay porridge production conditions that can increase the maximum enzyme resistance starch content is heated for 2 hours to 5 hours at 50 ℃ -56.5 ℃ I was able to arrange it.

[시험예 3] 전분분해율 시험Test Example 3 Starch Degradation Rate Test

상기 시험예에서 측정한 RS의 함량의 실제 전분분해율과의 연관성을 증명하기 위하여, 각 가열조건에 대해 하기와 같이 Singh 등의 방법(Singh, U., Kherdeka M.S. and Jambunathan, R. Studies on Desi and Kabuli chickpea (Cicer arietinum L) cultivars. The levels of amylase inhibitors, levels of oligosaccharides and in vitro starch digestibility. J. Food Sci. 47: 510-513 (1982))에 따라 α-아밀라제(α-amylase)를 이용하여 in vitro 전분분해율(in vitro starch digestibility; 이하 IVSD)을 측정하였다.In order to prove the correlation with the actual starch decomposition rate of the RS content measured in the above test example, the method of Singh et al. (Singh, U., Kherdeka MS and Jambunathan, R. Studies on Desi and Kabuli chickpea (Cicer arietinum L) cultivars.The levels of amylase inhibitors, levels of oligosaccharides and in vitro starch digestibility.Use of α-amylase according to J. Food Sci. 47: 510-513 (1982)). In vitro starch digestibility (hereinafter referred to as IVSD) was measured.

먼저, 포르신 α-아밀라제(porcine α-amylase)를 이용하여 전분을 분해하여 말토오스(maltose)를 유리시킨 후, 유리된 말토오스(maltose)를 3,5-디니트로살리싸이클산(3,5-dinitrosalicylic acid (DNS)) 시약(reagent)을 사용하여 540nm에서 흡광도를 측정하였으며, 전분분해율은 mg maltose/g(건물중량 기준)으로 하기 표 5에 나타내었다.First, maltose is liberated by decomposing starch using porcine α-amylase, and then liberated maltose is 3,5-dinitrosalicylic acid (3,5- Absorbance was measured at 540 nm using dinitrosalicylic acid (DNS)) reagent, and the starch decomposition rate was shown in Table 5 as mg maltose / g (based on the weight of the building).

타락죽의 전분분해율Starch decomposition rate of perilla 가열온도(℃)Heating temperature (℃) 가열시간(분)Heating time (minutes) 전분분해율(mg maltose/g동결건조분말)Starch decomposition rate (mg maltose / g freeze dried powder) 대조구Control 1010 142.70(10.80) 142.70 (10.80) 5050 240300240300 104.36(7.91) 103.99(13.10)104.36 (7.91) 103.99 (13.10) 56.556.5 120240120240 124.16(11.03) 120.16(15.73) 124.16 (11.03) 120.16 (15.73) 6464 6012060120 168.20(8.19) 151.75(9.35) 168.20 (8.19) 151.75 (9.35) 6969 30603060 188.11(5.11) 151.79(17.66) 188.11 (5.11) 151.79 (17.66)

a. 각 측정치는 3반복 평균임.a. Each measure is an average of three iterations.

b. 괄호안의 숫자는 p<0.05에서의 표준편차임.b. The numbers in parentheses are the standard deviation at p <0.05.

시험 결과, 가열온도 50℃에서 4시간 및 5시간 가열시 α-아밀라제(α-amylase)에 대한 IVSD는 건조분말 타락죽에 대하여 각각 104.36 mg maltose/g과 103.99 mg maltose/g을 나타내었으며, 56.5℃에서 2시간 및 4시간 가열시에는 각각 124.16 mg maltose/g와 120.16 mg maltose/g를 나타냄으로써, 142.7 mg maltose/g을 나타낸 대조구의 IVSD 보다 낮았다. 반면, 가열온도 69℃에서 30분과 60분 가열시 타락죽의 IVSD은 각각 188.11 mg/g과 151.79 mg/g, 그리고 64℃에서 1시간 및 2시간 가열시에는 각각 168 mg maltose/g과 151.79 mg maltose/g을 나타냄으로써 대조구보다 높았다.As a result of the test, IVSD for α-amylase after 4 hours and 5 hours heating at 50 ℃ showed 104.36 mg maltose / g and 103.99 mg maltose / g, respectively, for dry powder decay. At 2 and 4 hours heating at, it showed 124.16 mg maltose / g and 120.16 mg maltose / g, respectively, which was lower than the IVSD of the control group with 142.7 mg maltose / g. On the other hand, the IVSD of decayed porridge after heating for 30 and 60 minutes at 69 ℃ and 168.79 mg / g for 1 and 2 hours at 64 ℃, respectively, and 168 mg maltose / g and 151.79 mg maltose, respectively, at 64 ℃ / g was higher than the control.

이와 같은 결과를 전체적으로 볼 때, 가열조건에 따른 타락죽의 IVSD은 가열온도가 50℃, 56.5℃, 대조구, 64℃, 69℃인 순서로 증가하였으며, 각 가열온도에서는 가열시간이 증가할수록 감소함을 알 수 있었다.In conclusion, the IVSD of the decayed porridge according to the heating conditions increased in the order of 50 ℃, 56.5 ℃, control, 64 ℃, and 69 ℃, and decreased with increasing heating time at each heating temperature. Could know.

결과적으로, 상기 시험예 1에서 선정된 185℃에서 25분간 볶은 멥쌀가루를 이용하여 50℃-56.5℃에서 2시간-5시간 가열하는 본 발명의 타락죽 제조방법이 RS 함량 증진효과가 가장 클 뿐만 아니라 in vitro 전분분해율 역시 더 낮게 나타남으로써, 최대의 효소저항전분 함량 증진효과를 가져올 수 방법임이 증명된 것이라고 할 수 있다.As a result, the decay porridge production method of the present invention, which is heated for 2 hours to 5 hours at 50 ° C.-56.5 ° C. using non-glutinous rice flour roasted for 25 minutes at 185 ° C. selected in Test Example 1, has the greatest RS content enhancement effect. In vitro starch degradation rate was also lower, which proved to be the method that could increase the maximum enzyme resistance starch content.

이와 같이 완성되는 본 발명의 타락죽은 효소저항전분(Enzyme resistant starch: RS)의 함량이 최대화되고, 이에 따라 칼로리 기여도가 최소화됨으로써 최근 각광받고 있는 식이섬유 제품 및 다이어트 식품 등과 같은 기능을 가지는 성인용 건강식품으로 사용될 수 있게 되었다.The finished decayed porridge of the present invention maximizes the content of Enzyme resistant starch (RS) and accordingly minimizes the contribution of calories, thereby making adult health foods having such functions as dietary fiber products and diet foods. It can be used as.

이상과 같이 본 발명이 완성됨으로써, 멥쌀 1kg을 수세 후 가수하여 실온에서 2시간 침지하고 30분간 체에 받쳐 물기를 제거한 후, 방앗간에서 물과 소금을 넣지 않고 빻은 다음 두 손으로 가루를 비벼서 고르게 하고, 쌀가루가 덩어리지지 않고 균일하게 되도록 체(355㎛)를 내린 후 이렇게 습식제분한 멥쌀가루 300g을 네모난 팬 (28×35×4 cm)에 고르게 펴서 열풍건조기로 40oC에서 5시간 건조한 후, -20oC에서 냉동보관함으로써 볶음에 사용하기 위한 쌀가루를 제조하고; 건조한 쌀가루 40g을 원형의 팬(지름 21cm×높이 4cm)에 고르게 볶아질 수 있도록 5mm 이하의 두께로 얇게 편 후, 열풍건조기에서 185oC로 25분간 볶은 다음 1시간 실온에 방치하여 냉각시키고, 냉각된 볶음 쌀가루를 체(180∼355㎛)에 내려 진공 비닐백에 넣은 후 -20oC에서 냉동보관함으로써 볶음 쌀가루를 제조하고; 냉동보관 볶음 쌀가루 15g을 400mL 비커에 넣고 우유 150g을 가하여 잘 저어 분산시킨 다음 5분간 실온에 방치하고, 쌀가루-우유 혼합물이 담긴 비커를 항온 수조(water bath)에 담근 후 크램프로 고정하고, 비커 상부에 랩 교반기(Lab stirrer(MS-3030®, Tops, Korea))를 장치하고 임펠러(impeller)를 고정한 후, 저어주면서 죽의 내부온도가 50℃-56.5℃가 되도록 항온 수조(water bath)의 온도를 조절하여 2시간-5시간 동안 가열하고, 완료 5분 전에 0.5g의 소금을 넣고 다시 가열함으로써 타락죽을 제조하는 것으로 이루어지는, 효소저항전분 함량을 증진시키는 타락죽의 제조방법이 제공될 수 있게 되었다.As the present invention is completed as described above, 1kg of non-glutinous rice is washed with water, immersed in water for 2 hours, immersed in a sieve at room temperature for 30 hours, and then drained with a sieve for 30 minutes. After sieve (355㎛) to make rice powder uniform without mass, spread the wet powder 300g evenly on a square pan (28 × 35 × 4 cm) and dry it at 40 o C with a hot air dryer for 5 hours. Prepares rice flour for use in roasting by freezing at -20 o C; 40 g of dry rice flour is thinly sliced to a thickness of 5 mm or less so that it can be roasted evenly in a round pan (21 cm in diameter x 4 cm in height), and then roasted in a hot air dryer at 185 o C for 25 minutes, and allowed to stand at room temperature for 1 hour to cool. Prepared fried rice flour by lowering the fried rice powder into a sieve (180-355 μm) and placing it in a vacuum plastic bag and freezing at −20 ° C .; Freeze-stored Stir-fried rice powder 15g into a 400mL beaker, add 150g of milk, stir well, disperse and leave at room temperature for 5 minutes, soak the beaker containing the rice flour-milk mixture in a water bath and fix it with a cramped top Install a stirrer (Lab stirrer (MS-3030 ® , Tops, Korea)) on the top and fix the impeller, and then stir the temperature of the water bath so that the inside temperature of the porridge is 50 ℃ -56.5 ℃ It was possible to provide a method for producing perilla which improves the enzyme resistance starch content, which comprises adjusting perilla and heating for 2 hours to 5 hours, and adding perilla of 0.5 g of salt and heating again.

본 발명이 완성됨으로써, 타락죽의 원료가 되는 쌀의 호화 조건 및 이를 이용한 죽의 제조 조건을 최적화하여 효소저항전분(Enzyme resistant starch: RS)의 함량을 최대화하고, 이에 따라 칼로리 기여도를 최소화함으로써 식이섬유 식품 또는 다이어트 식품 등의 성인용 건강식품으로 사용될 수 있는, 타락죽의 제조방법이 제공될 수 있게 된 것이다. By the completion of the present invention, by optimizing the geological conditions of rice, which is the raw material of the decay porridge and the production conditions of porridge using the same, the content of Enzyme resistant starch (RS) is maximized, thereby minimizing the contribution of calories to dietary fiber It can be used as a health food for adults, such as food or diet foods, it can be provided a method of producing perilla.

Claims (1)

효소저항전분 함량을 증진시키는 타락죽의 제조방법에 있어서,In the production method of perilla to improve the enzyme resistance starch content, 멥쌀 1kg을 수세 후 가수하여 실온에서 2시간 침지하고 30분간 체에 받쳐 물기를 제거한 후, 방앗간에서 물과 소금을 넣지 않고 빻은 다음 두 손으로 가루를 비벼서 고르게 하고, 이렇게 습식한 멥쌀가루 300g을 네모난 팬(28×35×4 cm)에 고르게 펴서 열풍건조기에서 40oC에서 5시간 건조한 후 쌀가루가 덩어리지지 않고 균일하게 되도록 체(355㎛)를 내린 다음 -20oC에서 냉동보관함으로써, 볶음에 사용하기 위한 쌀가루를 제조하고;After washing 1kg of non-glutinous rice with water, it is immersed in water for 2 hours at room temperature, and then drained with a sieve for 30 minutes.Then, grind without adding water and salt in the mill, and then rub the powder with both hands to make a bowl. Spread evenly on egg pan (28 × 35 × 4 cm), dry at 40 o C for 5 hours in a hot air dryer, lower the sieve (355 μm) to make rice powder uniform and freeze, then stir-fried at -20 o C Preparing rice flour for use in the market; 쌀가루 40g을 원형의 팬(지름 21cm×높이 4cm)에 고르게 볶아질 수 있도록 5mm 이하의 두께로 얇게 편 후, 열풍건조기에서 185oC로 25분간 볶은 다음 1시간 실온에 방치하여 냉각시키고, 냉각된 볶음 쌀가루를 체(180∼355㎛)에 내려 진공 비닐백에 넣은 후 -20oC에서 냉동보관함으로써, 볶음 쌀가루를 제조하고;40 g of rice flour is thinly sliced to a thickness of 5 mm or less so that it can be roasted evenly in a circular pan (21 cm in diameter x 4 cm in height), and then roasted in a hot air dryer at 185 o C for 25 minutes and left to stand at room temperature for 1 hour to cool. To stir fried rice flour in a sieve (180 ~ 355㎛) and put in a vacuum plastic bag and frozen at -20 ° C, to prepare fried rice flour; 냉동보관 볶음 쌀가루 15g을 400mL 비커에 넣고 우유 150g을 가하여 잘 저어 분산시킨 다음 5분간 실온에 방치하고, 쌀가루-우유 혼합물이 담긴 비커를 항온 수조(water bath)에 담근 후 크램프로 고정하고, 비커 상부에 랩 교반기(Lab stirrer(MS-3030®, Tops, Korea))를 장치하고 임펠러(impeller)를 고정한 후, 저어주면서 죽의 내부온도가 50℃-56.5℃가 되도록 항온 수조(water bath)의 온도를 조절하여 2시간-5시간 동안 가열하고, 완료 5분 전에 0.5g의 소금을 넣고 다시 가열함으로써 타락죽을 제조하는 것으로 이루어짐을 특징으로 하는,Freeze-stored Stir-fried rice powder 15g into a 400mL beaker, add 150g of milk, stir well, disperse and leave at room temperature for 5 minutes, soak the beaker containing the rice flour-milk mixture in a water bath and fix it with a cramped top Install a stirrer (Lab stirrer (MS-3030 ® , Tops, Korea)) on the top and fix the impeller, and then stir the temperature of the water bath so that the inside temperature of the porridge is 50 ℃ -56.5 ℃ After adjusting for 2 hours to 5 hours, and 5 minutes before the completion of 0.5 g of salt, and by heating again, characterized in that the prepared 효소저항전분 함량을 증진시키는 타락죽의 제조방법.A method of preparing perilla to enhance the enzyme resistance starch content.
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