KR100523318B1 - Fungicidal composition containing tricyclazole and adjuvant - Google Patents

Abstract

The present invention relates to a composition of tricyclazole, a fungicide for cultivating rice, including a drug enhancing substance, which is a fungicide for cultivating rice, and according to the present invention, sodium dioctylsulfoxitrate and sodium dodecylbenzenesulfonate By using one or more surfactants selected from among anionic surfactants as drug-enhancing substances and adding them to tricyclazole products during the manufacture of agrochemical products and applying them to rice, the tricyclazole can be greatly improved in plant absorption. At the same time, by increasing the adhesion amount of tricyclazole to rice, it is possible to obtain a good control effect on the blast disease, and to extend the duration of the drug, and to significantly reduce the amount of tricyclazole used.

Description

Tricyclazole disinfectant composition containing drug-enhancing substance {FUNGICIDAL COMPOSITION CONTAINING TRICYCLAZOLE AND ADJUVANT}

The present invention is to control the rice blast of tricyclazole (5-methyl-1,2,4-triazolo [3,4-b] [1,3] benzothiazole), which is a pesticide currently used as an agricultural fungicide A tricyclazole sterilant composition comprising a surfactant that enhances the effect is provided.

In modern agriculture, pesticides are used as essential elements for the stable and mass production of high-quality crops. Hundreds of pesticides are currently manufactured and sold because their use varies according to crops and pests. Pesticides used for the protection of crops should be particularly effective in controlling weeds and pests. Recently, the problem of resistance to poor control effects due to the continuous use of pesticides has emerged.

Resistant weeds and pests need to be increased in pesticide use or replaced with new pesticides. Therefore, the industry may not only develop pesticides and mix them with other pesticides, but also improve the efficacy of existing pesticides. Efforts have been made to increase the effectiveness of the control by the addition of a substance that enhances efficacy.

Examples of additives for enhancing the efficacy of the pesticide include, for example, a humectant which allows the pesticide to adhere to the control object, and an electrodeposition agent or an attached pesticide that prevents the pesticide attached to the control object from being easily washed away by wind or rain. Water immersion enhancers that allow large amounts to penetrate at high rates. When applied to plants together with pesticides, a larger amount of pesticides are attached to the plants to express the effects, or the pesticides applied to the plants can be rapidly penetrated into the plant tissues in a large amount of time, thereby increasing the speed of expression and strength of the effects. In addition, it is possible to prevent the pesticides from being washed in the rainwater during the rainfall, thereby improving the efficacy of the drug stably. In particular, in the case of fungicides can often improve the effect as well as preventive effect on the disease, it is possible to obtain the effect of reducing the amount applied.

Meanwhile, according to the Pesticide Instruction Manual (published by the Pesticide Industry Association) and the Pesticide Manual (Crop production Publications), tricyclazole, which has long been the most used in the control of various blast diseases in rice farming, Although it is excellent in the prevention effect, it does not have a therapeutic effect, and it is described as the agent which is excellent in penetration performance. However, if only the product itself is diluted with water and sprayed, it does not adhere well to rice, it is not only slow to penetrate into the rice paddy, but also easily washed by rainwater during rainy weather due to its property of being soluble in water of active pesticides. Only a large amount of spray can be expected. This has attracted attention as a representative pesticide that pollutes water quality.

Tricyclazole is commonly used for the purpose of preventing various blast diseases through rice foliage treatment, but even when treated in paddy fresh water in the form of granules, it is also absorbed through the roots and exhibits the effect of preventing blast diseases. Therefore, when sowing seedlings on seedlings, a method of mixing with soil is also used. However, because it often causes rice damage, it is rarely used.

The fact that tricyclazole, which is treated in the form of granules in the soil, can have a protective effect against blasts, once the tricyclazole has entered the rice, it can be secreted outside the rice over time, thus preventing the blast. It is indirectly proving that it exists.

When tricyclazole products are used for spraying, we recommend the use of an electrodeposition agent to ensure that the pesticide adheres to the rice leaves, but rarely is it available in farms. In addition, even if the amount of adhesion to rice is increased by using an electrodeposition agent, most of the pesticides are very low because it is washed away by rainfall such as summer rain showers or rainy season.

Therefore, it is urgently needed to develop a product that adheres well to rice when sprayed on rice and is not easily washed at the same time as a result.

Techniques for selecting and adding to the formulation or tankmixing agents that can enhance the efficacy of tricyclazole are described in US Pat. No. 6,229,301 (Enhancement of the efficacy of triazolopyrimidines). In this patent, polyalkoxylated aliphatic alcohols, solid sodium hydrocarbyl sulfonates and polyalkoxylated trisiloxanes are selected as substances that can enhance the efficacy of various pesticides, including tricyclazoles. It is claimed that substances can enhance the efficacy of various pesticides regardless of the type of plant or plant disease. However, polyalkoxylated aliphatic alcohols are particularly resistant to fish at low concentrations and thus cannot be used in rice cultivation paddies. Polyalkoxylated trisiloxanes have been found to be resistant to rice as shown in the present study. Penetration rate is not very good.

The inventors have developed a device analytical method for selecting drug-enhancing substances that can enhance plant permeability of pesticides at low cost, effort and short time. Method of measuring the absorption rate, Korean Patent No. 0314600, Australian Patent No. 743453, and Japanese Patent No. 3348153).

By using this method, many kinds of candidates for pesticides and drug enhancers could be selected, and N- (α-cyano-2-tenyl) -4-ethyl-2- (ethylamino) -5-thiazolecarboxa was selected. Korean Patent Application No. 10-2000-0025096 for a novel fungicide containing mead and a surfactant composition for enhancing the absorbency of the fungicide dimethomorph to plant leaves and a Korean patent for a fungicide composition comprising the same Application 2001-0003269 has been filed.

The inventors then conducted a number of studies on drug-promoting substances and methods of using the tricyclazole, which greatly increases the adhesion to rice and at the same time enhances the permeability to the rice paddy, resulting in greatly improved disease control effects. As a result, the present invention has been completed.

Therefore, an object of the present invention is to improve the efficacy of the tricyclazole as a pesticide, that is, the effect of preventing itself against blast disease, and to greatly enhance the adhesion and absorption of rice after spraying, so that the blast control effect can be maintained for a long time. In addition to providing a tricyclazole disinfectant composition comprising a surfactant for enhancing drug efficacy, which is stable during formulation.

In order to achieve the above object, in the present invention,

(a) tricyclazole, and

(b) providing a disinfectant composition comprising at least one drug enhancing agent selected from sodium dioctylsulfosuccinate (hereinafter also referred to as 'SDSS') and sodium dodecylbenzenesulfonate (hereinafter also referred to as 'NaDBS'). .

In addition, the present invention provides a rice blast control method using the fungicide composition.

Hereinafter, the present invention will be described in more detail.

In the fungicide composition of the present invention, the ratio of the tricyclazole active ingredient and the drug-enhancing substance is preferably in the range of 1: 0.4 to 1:20 by weight.

The tricyclazole fungicide composition comprising the drug-enhancing substance according to the present invention may be directly formulated into any formulation form known in the art, for example, a one-pack formulation, such as a hydrate, granular or liquid hydrate, or the like. May be used.

Tricyclazole disinfectant composition comprising the drug-enhancing substance according to the present invention is preferably included in an amount ranging from 1 to 60% by weight, preferably 2.5 to 50% by weight of tricyclazole as an active ingredient. When 1% or less of tricyclazole as an active ingredient is used, the amount of the composition is too high. When the content exceeds 60% by weight, the content of the drug-promoting substance in the composition is relatively small, which may lower the effect of improving the drug efficacy. have.

Tricyclazole medicament enhancing substances according to the present invention are anionic surfactants or mixtures thereof including sodium dioctylsulfoxylcinate and sodium dodecylbenzenesulfonate. The drug enhancing substance is preferably included in an amount of 1 to 80% by weight, preferably 5 to 75% by weight in the disinfectant composition.

Among the drug enhancing substances according to the present invention, the material exhibiting the greatest drug efficacy may vary slightly depending on the form of the composition, such as a hydrating agent, a granulating hydrating agent, or a liquid hydrating agent, and if necessary, the medicinal enhancing substances may be mixed with each other.

When the tricyclazole pesticide formulation containing the drug-enhancing substance is diluted in water and sprayed on rice, the drug-enhancing substance of the present invention is increased from about 25 mg / L to the concentration of tricyclazole, but as the concentration increases, At a concentration of about 1,000 mg / l or more, it is preferable to use it at a lower concentration, since rice may be harmed by the drug-enhancing substance. Particularly, it is used within a concentration range of about 100 mg / l to about 500 mg / l. desirable. At this time, the concentration of tricyclazole may be in a concentration range of about 25 mg / L to about 375 mg / L.

Agrochemical compositions containing tricyclazole and the drug-enhancing substance for tricyclazole according to the present invention are known ingredients required in the formulation art, for example, water when formulated into different formulations such as a hydrating agent, a granulating hydrating agent or a liquid hydrating agent. Additives such as liquid and solid carriers, wetting agents, dispersants, and other stabilizers, adhesives, antifoaming agents, and the like may be included in conventional amounts, such as within 20% of wetting agents or dispersants.

Examples of what can be used as a solid carrier include powdered synthetic silica, diatomaceous earth, talc, leadstone, kaolin, anhydrous sodium sulfate, sodium benzoate, starch, sugars and the like.

In addition, although the drug-enhancing substance for tricyclazole selected in the present invention may serve as a wetting agent or a dispersing agent itself, other wetting agents or dispersing agents commonly used in the art may be used to improve the physical properties of the composition of the present invention. Can be used. Examples of these wetting agents or dispersants include polyoxyethylene nonylphenyl ethers, salts of polyoxyethylene alkylaryl ether sulfates, salts of polyoxyethylene alkylaryl ether sulfonic acids, dialkylsulfoxysinate salts, dodecylbenzenesulfonic acid salts, Ligninsulfonic acid salts, salts of sodium lauryl sulfate, and the like, but are not limited to those described above, and any material that helps the wettability and dispersibility of the formulation may be selected further from nonionic surfactants and anionic surfactants. You may use it.

The pesticide (disinfectant) composition according to the present invention is diluted in water and, as described above, in a concentration ranging from 25 mg / l to 375 mg / l of tricyclazole, and 100 mg / l to 1,000 mg / l of drug-enhancing substance. When sprayed on rice in the form of spray, it can effectively control rice blast.

The pesticide preparation of the present invention may be formulated in the form of a mixture including additional pesticides for the treatment of heat diseases other than tricyclazole, and as an additional pesticide that can be used, the therapeutic effect is excellent. Pesticides that easily degrade after application, for example, ferimzone (ferimzone, ( Z ) -2'-methylacetophenone 4,6-dimethylpyrimidin-2-ylhydrazone) and iprobenfos, S -benzyl O, O -diisopropylphosphothioate) and the like. Such agrochemicals are preferably contained in the formulation in an amount ranging from 1 to 5 times by weight of tricyclazole.

Hereinafter, the present invention will be described in more detail based on the following Examples and Test Examples. However, the following Examples and Test Examples are only for illustrating the present invention, but the scope of the present invention is not limited thereto.

Example 1 Preparation of Drug-Promoting Agent Composition

Sodium dioctylsulfosuccinate (from Aldrich, purity 98%) and sodium dodecylbenzenesulfonate (from Aldrich), respectively, as shown in Table 1, isopropyl alcohol (hereinafter also referred to as 'IPA') It was dissolved in and distilled water was added to prepare a drug enhancer.

This was used in subsequent blast control effect measurement, rice absorption of tricyclazole hydration and adhesion test.

Ingredient Name Composition (%) and Physical Properties of Drug Enhancers for Tricyclazole Liquid 1 Liquid 2  SDSS 50 -  NaDBS - 50  Solvent or carrier 25 (IPA) 25 (water) 25 (IPA) 25 (water)  Solubility Dissolution Dissolution  Formulation stability stability stability

Example 2: Preparation of Drug-Promoting Substance-containing Tricyclazole Compositions

Tricycle containing a tricyclazole raw agent, sodium dioctylsulfoxysinate or sodium dodecylbenzenesulfonate and powder carrier as shown in Table 2 to Table 3, followed by grinding Sol hydrates were formulated.

In order to test the physical properties of the tricyclazole hydrating agent containing the drug-enhancing substance, 200 ml of hardened water was added to a beaker (500 ml), and 0.2 g of the pesticide formulation was dropped, and water was observed to be wet. After 5 minutes, the mixture was gently stirred with a glass rod, and the dilution state was observed. Pesticide formulations were stored at room temperature for 3 months and observed for state changes. The composition and physical property test results of the respective hydrating agents are shown in Tables 2 and 3.

Ingredient Name Composition of Tricyclazole Hydrating Agent Including Drug-Enhancing Substance (% by Weight) Hydration 1 Hydration 2 Hydration 3 Hydration 4 Hydration 5 Hydration 6 Hydration 7 Tricyclazole Original (Purity 97%) 51.5 41.2 25.8 20.6 20.6 10.3 2.6 SDSS 25.0 40.0 50.0 44.4 54.0 50.0 50.0 Sodium benzoate 7.5 18.8 15.0 13.3 16.2 15.0 15.0 kaoline 16.0 - 9.2 21.7 9.2 24.7 32.4 Diluent State suspension suspension suspension suspension suspension suspension suspension Formulation stability stability stability stability stability stability stability stability

Ingredient Name Composition of Tricyclazole Hydrating Agent Including Drug-Enhancing Substance (% by Weight) Hydration 8 Hydration 9 Hydration 10 Hydration 11 Hydration 12 Hydration 13 Hydration 14 Tricyclazole source (purity 97%) 51.5 41.2 25.8 20.6 20.6 10.3 2.6 NaDBS 25.0 40.0 50.0 44.4 54.0 50.0 50.0 Synthetic Silica (Ti _x -O-sil) 5.0 10.0 10.0 10.0 10.0 10.0 10.0 kaoline 18.5 8.8 14.2 25.0 15.4 29.7 37.4 Diluent State suspension suspension suspension suspension suspension suspension suspension Formulation stability stability stability stability stability stability stability stability

Example 3 Preparation of Drug-Promoting Substance-containing Tricyclazole-Pesticide Mixture Compositions

As shown in Table 4, the tricyclazole raw agent, the ferrim zone raw material, the iprobenfoss raw material, sodium dioctylsulfoxylcinate or sodium dodecylbenzenesulfonate and powder carrier are mixed and ground, the drug-enhancing substance according to the present invention A tricyclazole-pesticide combination wetting agent was prepared.

In order to test the physical properties of the combined hydration agent containing the drug-enhancing substance, 200 ml of hard water of 3 ° C. was placed in a beaker (500 ml), and 0.2 g of the pesticide formulation was dropped and then wet with water. After 5 minutes, the mixture was gently stirred with a glass rod, and the dilution state was observed. Pesticide formulations were stored at room temperature for 3 months and observed for state changes. Table 4 shows the composition and the physical property test results of each hydrating agent.

Ingredient Name Composition of Tricyclazole-Pesticide Mixture Hydrating Agent Containing Drug-Enhancing Substance (% by Weight) Hydration 15 Hydration 16 Hydration 17 Hydration 18 Comparator 1 Tricyclazole Original (Purity 97%) 10.3 10.3 10.3 5.2 10.3 Ferrim Zone Original (Purity 98%) 30.6 30.6 - - 30.6 Ifprobenfoss raw material (purity 95%) - - 21.1 21.1 - SDSS 27.0 - 27.0 - - NaDBS - 27.0 - 27.0 - Polyoxyethylene nonylphenyl ether - - - - 2.0 Polyoxyethylene Nonylphenyl Ether Sulfate - - - - 3.0 Sodium benzoate 8.1 - 8.1 - - Synthetic Silica (Tix-O-sil) - 5.0 - 5.0 5.0 kaoline 24.0 27.1 33.5 41.7 49.1 Diluent State suspension suspension suspension suspension suspension Formulation stability stability stability stability stability stability

In addition, the tricyclazole disinfectant composition comprising the surfactant for enhancing drug efficacy of the present invention not only has excellent heat-control control effect of the surfactant itself, but also the amount of tricyclazole adhered to rice by the surfactant for enhancing drug efficacy. Increasing the penetration rate and increasing the amount of active ingredients remaining in rice during the rainy season during which the blast occurs mainly, thereby greatly improving the blast control effect of tricyclazole and prolonging the duration of the medicinal effect, resulting in stable drug expression and simultaneous use. Can be greatly reduced. Hereinafter, the effects of the present invention will be described in detail through test examples.

Experimental Example 1 Measurement of Anti-Therapeutic Effect of Drug Enhancer (Surfactant) Composition

Young Nak-dong rice was planted for 3 weeks in pots (66mm in diameter and 66mm in height), and it was planted up to 4 leaves in the greenhouse, and only samples with uniform growth conditions were used for measuring the anti-heating effect of surfactant for enhancing drug efficacy. Rice bran was inoculated into rice bran medium (20 g of rice bran, 10 g of dextrose, 16 g of agar, 1 L of distilled water) and incubated with shaking at 25 ^° C. for 7 days. This was changed by a mixer and put on rice bran medium again and incubated for 14 days in an incubator at 25 ^o C. Two days prior to inoculation of rice spores with rice, the bacteria were scraped off the grown media and wounded for 48 hours under fluorescent lamps. Sterile distilled water was added and the spores were scraped and harvested. Then, the spore concentration was adjusted to a proper concentration (5 × 10 ⁵ spores / ml) using a hemocytometer to prepare a spore suspension for use as a heating bottle inoculator.

The liquid solution of Example 1 was diluted in water to prepare a spray solution containing 250 mg / l, 500 mg / l and 1,000 mg / l of the surfactant as an active ingredient, respectively. Five pots of rice were placed in a track sprayer (Spray Booth Model SB-6, R & D Sprayers Inc.), sprayed at a level of 1,000 L / ㏊, and dried at room temperature. 100 ml of spore suspension was spray inoculated per 24 pots of rice. It was placed in a dark room at 25 ^o C for 24 hours and then transferred to a constant temperature and humidity room with a relative humidity of 80% or more and left for 4 days. After investigating the area ratio of the lesions formed on the rice leaves, Equation 1 was used to calculate the effect of controlling the blast disease, and the results are shown in Table 5.

Control price (%)

= (Length area ratio of treatment-free treatment area-Lesion area ratio of treatment treatment) / Lesion area ratio of treatment-free treatment * 100

Main ingredient (formulation number) Effect of prevention of blast disease by concentration (%) 250 mg / l 500 mg / l 1,000mg / ℓ  SDSS (Liquid 1) 29 84 93  NaDBS (Liquid 2) 93 100 100

As shown in Table 5, SDSS and NaDBS selected as a surfactant for improving efficacy of the fungicide pesticide tricyclazole in the present invention have a high preventive effect of over 80% at a concentration of 500 mg / l or more without containing tricyclazole. Indicated. It is clear that such blast control will be effective in enhancing the efficacy of tricyclazole.

Test Example 2: Measurement of the Blast Prevention Effect of Surfactant for Enhancing Drug Effects by Inoculation Concentration

Using the same method as Test Example 1 and varying only the inoculation concentration of the blast spores, the effect of blast prevention was measured, and the results are shown in Table 6.

Main ingredient (formulation number) Surfactant Concentration (mg / ℓ) Effects of Red Pepper Plague Prevention According to Inoculation Concentration (%) 5 × 10 ^5a 1 × 10 ⁶ 2 × 10 ⁶  SDSS (Liquid 1) 250 56 33 10 500 84 50 34  NaDBS (Liquid 2) 250 95 96 90 500 100 99 98 a Spore inoculation concentration (unit: migrant / ml)

As shown in Table 6, as the concentration of blast spores was increased, the blast control effect of SDSS was somewhat lowered, but NaDBS showed a high control effect of 90% or more even at the concentration of 250 mg / L.

Experimental Example 3: Measurement of Duration of Anti-Therapeutic Effect of Surfactant for Pharmacokinetic Enhancement

Using the same method as Test Example 1 and varying the inoculation time of the blast spores after spraying the drug was measured for the prevention of blast disease, the results are shown in Table 7.

Main ingredient (formulation number) Effect of spore inoculation on spraying spores after drug application 1 day 3 days 7 days 10 days  SDSS (Liquid 1) 1,000 mg / l 100 100 100 100  NaDBS (Liquid 2) 500 mg / l 100 100 100 100

As shown in Table 7, the tricyclazole drug enhancers SDSS and NaDBS of the present invention exhibited a 100% anti-foam prevention effect even after 10 days of spray treatment, and the drug duration was very long.

Test Example 4: Determination of Rice Leaf Permeability and Rice Adhesion of Tricyclazole Hydrate Containing Drug-Enhancing Substances

Young Nak-dong rice was planted for 3 weeks in pots (66 mm in diameter and 66 mm in height), and it was grown to 7 leaf stages in the greenhouse, and only samples with uniform growth conditions were used for the measurement of rice leaf penetration rate and rice adhesion rate of tricyclazole hydrate.

Diluting the hydration agent 5 and the hydration agent 12 of Example 2 in water and mixed with an aqueous solution of Congo Red (products of Cond. Spray dilutions of 1,000 mg / l of active agent and 100 mg / l of Congo Red were prepared. Tricyclazole hydrating agent (trade name: Trizol, agro-products) was used as a control agent, and the control electrodeposition agent polyalkoxylated trisiloxane, which was used as a drug-enhancing substance in the United States patent, is an electrodeposition agent in the market Products purchased (silhouette, Silwet L-77, Dongbu Hannong Chemical Products) were used.

10 pots of rice was added to a track sprayer (Spray Booth Model SB-6, R & D Sprayers Inc.) and the suspension was sprayed to 250 l / dl. Immediately after spraying, 5 pots of samples were taken, cut into rice fields, placed in a test tube (inner diameter 32 mm, length 200 mm), 10 ml of 30% acetonitrile solution was added, capped and inverted and shaken at a speed of 60 times per minute for 2 minutes. The rice was taken out, transferred to a stoppered test tube, and sealed. The remaining rice was stored in a dark growing box maintained at a temperature of 25 ± 0.5 ^o C and a relative humidity of 80 ± 5%. After 24 hours of storage, the resultant was washed in the same manner as above to obtain a washing solution, and then analyzed by HPLC to calculate tricyclazole adhesion amount and penetration rate. The results are shown in Table 8.

Test medication Rice leaf penetration rate (%) Rice attachment index (%) ^a Pharmacokinetic effect ^b Hydration 5 52.5 ± 0.5 4.4 ± 0.5 18.0 Hydration 12 36.8 ± 2.5 5.5 ± 0.4 15.8 Antidrug + Silhouette 13.0 ± 1.5 1.5 ± 0.3 1.5 Reference drug 12.8 ± 1.9 1.0 ± 0.2 1.0 a Relative comparison of adhesion amount with no surfactant added to 1b Multiplying rice leaf index by rice adhesion index and dividing by reference agent

As can be seen from the above table, the tricyclazole hydrate (control agent) containing no surfactant for enhancing efficacy of rice leaf penetration was only 12.8% after 24 hours of drug treatment, while the hydrating agent containing drug enhancing substance was 52.5. Significantly increased to 3% and 36.8%.

In addition, when the amount of adhesion of the hydration agent containing the drug-enhancing substance was increased to 5.5, the amount of adhesion of the tricyclazole hydration agent containing no drug-enhancing substance was increased to 5.5 times.

In addition, the effective amount that can penetrate inside the rice and remain in the rice for a long time without being lost by rainfall, and have a control effect against the blast bottle, is calculated by comparing with the hydration treatment that does not contain the drug-enhancing substance, up to 18.0 times Has been shown to be enhanced.

On the other hand, the commercially available silhouette (polyalkoxylated trisiloxane) hardly enhanced the penetration rate of tricyclazole, and also had a small amount of adhesion, indicating little effect on the disease control effect.

Test Example 5: Determination of the heat-controlling disease control effect of tricyclazole-ferrimzone combination hydrating agent containing drug-enhancing substance (indoor experiment)

Nakdong rice was planted in pots of 9 cm in diameter and 11 cm in height for 3 weeks and grown in a greenhouse. From the 5 ~ 6 leaf stage, dilute the hydration agent 17 and the control agent 1 of Example 3 to prepare a spray diluent of the composition shown in Table 6, and then use the track spray at a level of 1,000 l / ㏊ at intervals of 7 days. Sprayed.

The treated rice was stored for 24 hours in a wet room at 25 ^o C and a relative humidity of 80%, and then artificially rainfall to 20 mm using an artificial rainfall system, and cultivated by irrigating the bottom in the greenhouse. Four weeks after the initial drug treatment, the blast spore suspension was sprayed over the rice and cultivated in a wethouse provided in a greenhouse.

After 7 days of cultivation on the wetland, the lesion area ratio was examined by a crown tube irradiation, and the control value of the drugs was calculated by Equation 1 and then shown in Table 9.

Sample Number Composition of Drug (mg / L) Effect of Controlling Blast Disease on Cultivation Period after Treatment (%) 1 day 1 week 2 weeks 4 Weeks Comparator 1 Tricyclazole (50) + Ferrim Zone (150) 100 89 13 0 Hydration 17 Tricyclazole (50) + Ferrim Zone (150) + SDSS (135) 100 99 71 46

As shown in Table 9, the tricyclazole-ferrimzone combination significantly reduced the control effect on the blast after two weeks of treatment, but when sodium dioctylsulfosuccinate was added as a drug-enhancing substance for a longer period of time The disease control effect was maintained. This is because sodium dioctylsulfosuccinate has significantly increased the amount of adhesion to rice and its leaf permeation rate.

Test Example 6 Measurement of Rain Resistance of Tricyclazole Hydrating Agent Added with Drug-Enhancing Substance

Nakdong rice was sown and cultivated as used in Test Example 1 and used as a test rice sample when there were about two powdered liquors. The tricyclazole hydrate was diluted with distilled water to prepare a control drug suspension having an active ingredient of 450 mg / L. In addition, the water hydrate 4 of Example 2 was diluted in water to prepare a test suspension having an active ingredient of 450 mg / L and sodium dioctylsulfoxysinate 1,000 mg / L.

This suspension was sprayed to rice at a level of 1,000 l / dl using a track sprayer. Five pots were taken immediately after the treatment, and the rice surface was washed with a 30% acetonitrile aqueous solution in the same manner as in Test Example 4. The remaining rice was stored in a room having a temperature of 24 ^° C. to 25 ^° C. and a relative humidity of 70% to 80%. After 24 hours of storage, five of the rice samples were again taken and washed in the same manner. The remaining rice samples were cultivated with 20 mm artificial rainfall, the surface of the leaves was washed, and then irrigated in a greenhouse. After 3, 6, 9, 12, and 15 days of artificial rainfall, 5 pots of rice were taken to wash the top of the rice. The solution obtained by washing rice was analyzed by HPLC to calculate the ratio of the active ingredient penetrating into the rice paddy field after 24 hours and the amount of the active ingredient remaining on the surface of rice leaves, and the results are shown in Table 10 below.

Content of drug (mg / L) Penetration rate (% / 24 hours later) Tricyclazole Residue on Rice Leaf Surface after Artificial Rainfall (㎍ / pot) Right after spraying Right after rainfall 3 days later 6 days later 9 days later 12 days later 15 days later Tricyclazole Hydrating Agent (450mg / l) 12.4 9.6 ± 2.4 0.96 ± 0.1 0.68 ± 0.3 0.52 ± 0.2 0.35 ± 0.1 0.21 ± 0.1 0.31 ± 0.1 Tricyclazole (450 mg / l) + SDSS (1,000 mg / l) 34.3 55.8 ± 9.9 5.1 ± 1.2 3.1 ± 0.7 3.4 ± 0.6 3.5 ± 0.5 3.1 ± 0.6 2.1 ± 0.5

As can be seen in Table 10, the amount of the active ingredient attached to the rice paddy ground immediately after spraying the tricyclazole hydration agent added with sodium dioctylsulfosuccinate as the drug-enhancing substance does not contain the drug-enhancing substance. It was five times higher than when treated with medication.

Even after artificial rainfall, the rice treated with the hydration agent to which the drug-improving substance was added remained 5 times more active than the rice treated with the control agent. In addition, as the time passed after artificial rainfall, the residual amount of active ingredient in rice treated with the control agent decreased continuously, whereas in rice treated with tricyclazole hydrous added with sodium dioctylsulfosuccinate, after 6 days, it was a small amount. The residual amount of active ingredient in the ground showed a tendency to increase, which was confirmed by retesting.

It is known that tricyclazole's protective effect against blasting is well expressed even at extremely low active ingredient concentrations. Tricyclazole formulations containing drug-improving substances not only significantly increase the amount of adhesion to rice when treated, but also significantly increase the penetration rate, resulting in a large amount of active ingredient remaining in rice even after rainfall and a certain period of time. Afterwards, the amount of the active ingredient on the leaf surface is increased, so that the control effect on the blast can last longer than the control agent containing no potentiating substance.

Test Example 7: Test for control of the blast disease of tricyclazole-ferrimzone combination hydrate containing a drug enhancing substance (non-packaging test)

Nakdong rice was transplanted to paddy fields and cultivated for 1.5 months, and then used as a test package to measure the effectiveness of leaf blast control. Wetting agent 17 of Example 1 and Control Agent 1 were diluted in water and sprayed to the test package at a level of 1,000 l / dl using two batch sprayers at 7 day intervals.

After the first 40 days of treatment, the disease area ratio was investigated and the effect of controlling the blast disease was calculated by Equation 1. In addition, at the same time to investigate whether the weakness and the results are shown in Table 11.

Sample Number Composition of Drug (mg / L) Control effect of febrile disease Weakness (%) Comparator 1 Tricyclazole (100) + Ferrim Zone (300) 81.0 none Comparator 1 Tricyclazole (50) + Ferrim Zone (150) 72.2 none Hydration 17 Tricyclazole (50) + Ferrim Zone (150) + SDSS (135) 86.7 none

As can be seen in Table 11, the control effect of leaf blight of the tricyclazole hydrating agent added with sodium dioctylsulfosuccinate as the drug-enhancing substance was much higher than the control batch treatment without sodium dioctylsulfosuccinate. It is also shown that there is a statistically significant difference, and it is proved that the drug efficacy of tricyclazole is greatly enhanced by the drug-enhancing substance.

According to the present invention, an anionic surfactant containing at least one of sodium dioctylsulfoxysinate and sodium dodecylbenzene sulfonate as a drug enhancer of tricyclazole is employed to commercialize conventional fungicide tricyclazole. When the product is added to the product in the process and the product is diluted in water and sprayed on rice, it is possible to enhance the prevention effect of the tricyclazole by the thermal control of the surfactant itself, as well as the adhesion and permeability of the tricyclazole on the ground. In addition, the antimicrobial effect of the antimicrobial tricyclazole against rice blasts can be greatly improved, and the duration of the drug can be extended, resulting in a significant reduction in the amount of the drug used.

Claims (3)

Drug enhancing agents comprising anionic surfactants selected from sodium dioctylsulfoxyluccinate and sodium dodecylbenzenesulfonate, and tricyclazole (5-methyl-1,2,4-triazolo [ 3,4-b] [1,3] benzothiazole) in a weight ratio ranging from 0.4: 1 to 20: 1. The method of claim 1, In addition to tricyclazole, a sterilant composition, further comprising a medicament for controlling the blast. The fungicide composition according to claim 1 or 2 is prepared in the form of a spray solution in the range of 25 mg / L to 375 mg / L of tricyclazole concentration and 100 mg / L to 1,000 mg / L of a drug-enhancing substance concentration. A rice paddy control method, characterized in that sprayed on.