KR100504376B1 - Oxytocic and Method of Preparing the Same - Google Patents

Abstract

Disclosed are healthy foods and methods for preparing the same, which are prepared from herbal ingredients and have excellent efficacy and stability. Deer antler, Angelica, and Cheongung are included in a weight ratio of 0.8 to 4: 0.5 to 4: 1, and may have a food acceptable formulation such as powder, sugar solution or pills. This is prepared by the method of preparing the antler, Angelica, and Cheongung and mixing it with a predetermined amount of the extract, heating the resulting mixture to a temperature above the boiling point of the extract, and then heating and concentrating and cooling the mixture at a boiling point of ± 10 ° C. Can be prepared. It replaces the function of oxytocin hormone and naturally induces stable and fast delivery through uterine contraction, so it is expected to be a supplementary food that can eliminate side effects such as carcinogenic potential of hormonal preparations, fetal death and excessive uterine bleeding in pregnant women. .

Description

Healthy food and method of preparing the same for pregnant women

The present invention relates to a health food for pregnant women and a method for manufacturing the same, and in particular, a health food for pregnant women, which may be used as an alternative to oxytocin hormones, which are formed from extracts of herbal compositions such as deer antler, angelica and scorpio. And it relates to a manufacturing method thereof.

Recently, the economic level and quality of life have been improved, and medical devices are being advanced. The scientific verification that fetus and mother are suffering from the side effects by the delivery process that has been carried out through the existing artificial methods even in the obstetrical delivery process Research and attention has been focused on finding a safe delivery process through methods such as natural delivery rather than conventional methods such as surgery and the use of hormonal agents.

In general, the most representative of the methods aimed at promoting labor is to use oxytocin hormone (oxytocin hormone) as a delivery accelerator. Oxytocin hormone is widely used as a labor promoting agent in childbirth in the clinic by enhancing the number of contractions and contractility.

However, this may cause problems of over-uterine contraction, excessive pain of the mother, over-uterine bleeding, and overdosage, which may pose a threat to the life of the mother and the fetus if the mother is in poor health. In addition, oxytocin hormone acts on both pregnancy and non-pregnant uterine contractions, which can be interpreted as damaging to the selective natural physiological mechanism because of physiological analysis. have.

Therefore, the present invention is a stable herbal medicine made of a combination of herbal composition with a certain composition ratio such as antler, donkey, uterus and the like directly acting on the improvement of kidney function and activation of the uterus during childbirth in order to prevent the problems and side effects of the conventional method. It is an object to provide a health food for pregnant women having a composition.

Another object of the present invention is to provide an easy method for preparing a healthy food for pregnant women.

In order to achieve the above object, the present invention comprises a deer antler, Angelica and uterus in a ratio of 0.8 to 4: 0.5 to 4: 1 by weight and having a food-acceptable formulation such as powder, lactose or pills Provide health food for women.

The object of the present invention described above is achieved by a health food containing a antler and a uterus in a weight ratio of 0.8 to 4: 1, and having a food-acceptable formulation such as powder, lactose or pill, It is contained in a weight ratio of 0.5 to 4: 1, and is also achieved by a health food having a food-acceptable formulation such as powder, sugar solution or pill, and a ratio of 0.8 to 4: 0.5 to 4 in weight ratio of antler and angui. It is also achieved by a health food having a pharmaceutically acceptable formulation, such as powders, lactose or pills. The above object of the present invention is also achieved by a health food comprising antler and having a food acceptable acceptable formulation such as powder, lactose or pill.

These pregnant women's health foods can have all of the powder, lactose, pill formulations and the like.

Another object of the present invention described above

Preparing a deer antler, a donkey and a uterus in a weight ratio of 0.8 to 4: 0.5 to 4: 1, and mixing them with a predetermined amount of extract;

Heating the mixture to a temperature above the boiling point of the extract;

Concentrating by heating at a boiling point ± 10 ° C. of the extract;

Cooling the concentrate; And

It is achieved by a method for producing a healthy food for pregnant women, comprising the step of preparing the cooling liquid in a food acceptable formulation such as powder, lactose or pills.

In particular, water, alcohol, or the like may be used as the extract. The resulting coolant may be dried to prepare in the form of water-soluble granules. It is also possible to further perform the step of freeze drying to dry the coolant.

The health food according to the present invention may be prepared in the form of powder by preparing a deer antler, Angelica and Cheongung in a ratio of 0.8 to 4: 0.5 to 4: 1 by weight. The powder obtained may be taken with water or may be taken as a beverage in water or the like.

In this case, the white powder or crude syrup may be mixed with the powder to prepare a pill. In this case, the amount of the white wheat or crude syrup is preferably in the range of 15 to 20 parts by weight of the powder.

In the present invention, in order to minimize the side effects and damages caused by the use of hormones and to maintain a safe delivery process, in particular, the side effects of childbirth, such as excessive bleeding and ovary with the oxytocin hormone preparations to eliminate the side effects of maternity after childbirth and gynecology and kidney As a result of repeated research focused on strengthening uterine function, herbal extracts were developed to induce optimal labor.

 In addition, the present invention is prepared by including the herbal composition described above and adding a food-acceptable excipient such as a conventional powder, a tear solution or a pill and formulated by a food-acceptable method such as a conventional powder, a tear solution or a pill. It is to provide a variety of foods and methods for manufacturing the same that can be used for the treatment and prevention by promoting the delivery and replacement of oxytocin hormone.

Hereinafter, the present invention will be described in more detail.

In oriental medicine, the fidelity of renal function is very important in the birthing process of the mother. The uterus and bladder are directly related to the same functional group of the kidney, so the interaction and function are very closely related.

The present inventors have found that a mixed composition of antler, Angelica, and horoscope has a more physiologically stable function than oxytocin hormone and promotes delivery without any toxicity. In particular, antler is a component that enhances the function of the kidney and helps hormones, which itself can perform the function of promoting labor alone. The donkey not only helps the liver and kidneys but also acts as a blood-reducing agent to replenish the blood deprived by childbirth. Cheongung has a blood effect that helps the circulation of blood smoothly, and facilitates the role of delivery. Due to the characteristics of each of the three drugs, the combination of any two of the three to obtain the effect of showing the body of a pregnant woman, in the case of antler was confirmed that the effect appears to be used alone. However, the best effect is obtained when all three components are mixed.

The preparation method in a drinkable form using such a combination of herbal medicines can be any food-acceptable formulation, such as powder, sugar solution or pills without particular limitation. Examples include powders, sugars, and pill formulations.

The herbal extract used in the experiment was prepared by the following method. Three kinds of antler, Angelica, and Cheongung were finely pulverized and heated with hot water at a temperature of 100 ° C. for 1.5 to 3 hours to sufficiently extract the chemical liquid, filter, and freeze-dried the filtrate.

Hereinafter, the present invention will be described in more detail with reference to specific examples and experimental examples.

<Example 1>

75 g of antler, 56.25 g of Angelica vulgaris, and 37.5 g of uterus were put in 1.5 L of hot water and extracted at a temperature of 100 ° C. for 3 hours. After filtration, the filtrates were combined and lyophilized.

Experimental Example 1

According to the method of Voipio and Nevalainen, SD rats and non-pregnant rats whose pregnancy was confirmed were divided into three groups of the present invention extract, oxytocin hormone-administered group, and control group. After equilibrating, the number of uterine contractions and uterine contractile force were measured by applying a solvent, an invention according to each concentration, and an oxytocin hormone. Six experiments were performed: non-pregnant uterine contractions, 3 groups of contractile forces, 3 groups of pregnant uterus, and 3 groups of contractile forces.

Each test group was written as follows.

1.Control group (pregnant, nonpregnant)

2. Oxytocin hormone group (pregnant, nonpregnant)

3. Deer Antler, Angelica, Cervix Extract Administration Group (Pregnant, Non-Pregnant)

* Non-pregnant group uterine extraction and contraction test

6-estradiol benzonate 40µg / 0.2ml / body / day was injected subcutaneously for 2 days prior to the test, and the estrus-induced rats were killed by cervical distal bone and then immediately opened the lower abdomen. After removing the uterus, blood vessels and connective tissue around the uterus were removed, and the uterus closest to both uterine angles was also cut to a length of 1.5 cm. It was suspended in an organ bath and then connected to a transducer (PTr 300, Grass, U.S.A.) and a polygraph (polygraph, powerlab 400, ADinstruments, U.S.A.).

After equilibrating the tissues for 60 minutes, 100 μl of the preparation solvent, the invention extract for each concentration, and oxytocin were applied to the organ bath for 10 minutes to measure the degree of uterine contractility and the number of contractions per hour. Lock-Ringer Solution was used as the nutrient solution in the organ tank, and the temperature in each organ tank was maintained at 37 ° C. The nutrient solution gas was mixed with carbogen gas (95% O ₂ , 5% CO ₂ ). It was used, the resting tension (resting tension) was 1g.

* Pregnant Uterus Extraction and Contraction Test

After 17-18 days of gestation, rats were killed by cervical vertebral bones, and then immediately opened the lower abdomen to quickly remove the uterus, and then the fetus contained in the uterus was removed, and the blood vessels and connective tissues around the uterus were removed. The nearest uterus was cut to 1.5 cm long. It was suspended in an organ bath and then connected to a transducer (PT300, Grass, U.S.A.) and a polygraph (polygraph, powerlab 400, ADinstruments, U.S.A.).

After equilibrating the tissues for 60 minutes, 100 μl of the preparation solvent, the invention extract for each concentration, and oxytocin were applied to the organ bath for 10 minutes, and the degree of uterine contractility and the number of contractions per hour were measured. Lock-Ringer Solution was used as the nutrient solution in the organ bath, the temperature in each organ bath was maintained at 37 ° C, and the mixed gas used as the mixed carbogen gas (95% O ₂ , 5% CO ₂ ) was used. The resting tension was 1 g.

* Preparation and administration of test substance

After freeze-drying the liquid phase of the herbal extract of the invention extract using a lyophilizer, the test substance and oxytocin, which became a solid powder, were 0.1, 1, 10, 30mg / ml and 0.01, 0.1, 1U / The solution was used by dissolving in Lock-Ringer Solution at the concentration of ㎖. In order to induce the gestational age of non-pregnant group, 6-estradiol benzoate was dissolved in ethanol at the dose of 40µg / 0.2ml / body / day before the test. Subcutaneous injections were made for 2 consecutive days. The composition of the Lock-Ringer Solution is as follows (ml / L, NaCl: 154, MgCl ₂ .6H ₂ O: 2.1, CaCl: 2.6, NaHCO ₃ : 3.4, Glucose: 2.8). The Lock-Ringer Solution was adjusted to pH 7.4 and then saturated with mixed carbogen gas (95% O ₂ , 5% CO ₂ ).

Experimental group

① Control group (before each test substance treatment)

② invention extract single treatment group

Test group Preparation concentration (mg / ml) Actual concentration (pg / ml) after organ intratreatment Extract of Example 1 Extract of Example 1 Extract of Example 1 Extract of Example 1 0.111030 110100300

③ oxytocin alone treatment group

Test group Formulation Concentration (U / mL) Actual concentrations after treatment in organ crude (mU / ml) Oxytocinoxytocinoxytocin 0.010.11 0.1110

Test Animals and Breeding Conditions

All Spergue-Dawley rats weighing about 200 grams were supplied from Cheil Corp. for the entire testing period in an animal breeding room where the contrast was controlled at 12 hour intervals with a temperature of 23 ± 1 ° C, a relative humidity of 55 ± 15%, and 300 to 500 Lux. During the experiment, five animals were placed in a polycarbonate breeding box (240W x 39L x 175H mm) and purified for at least one week, and then observed with the naked eye. Only normal animals were used for the test. Intake.

The results are shown in Table 3 below.

Effect of the extract treatment of Example 1 on the contraction frequency and contractility of the non-pregnant excision uterus Test group Shrinkage (%) Contraction force (%) Extract of Control Example 1 1 μg / mL Extract of Example 1 10 μg / mL Extract of Example 1 100 μg / mL Extract of Example 1 300 μg / mL Oxytocin 0.1 mU / mL Oxytocin 1 mU / mL Oxytocin 10 mU / Ml 100.0 ± 0.089.6 ± 3.282.4 ± 3.387.6 ± 3.082.4 ± 3.3158.3 ± 0.6 ^* 308.3 ± 0.7 ^* 368.8 ± 0.7 ^* 100.0 ± 0.0101.1 ± 3.9101.6 ± 3.0103.0 ± 3.596.8 ± 2.4110.2 ± 1.5 ^*** 119.6 ± 1.8 ^*** 122.4 ± 2.1 ^***

Data expressed in ± are mean values obtained from 3-5 animals.

^* (P <0.05), ^*** (P <0.001): significantly different from control group.

From the above experimental results, it was confirmed that the extract according to Example 1 of the present invention has a selective characteristic without affecting the non-pregnant uterus. However, the oxytocin hormone affects the non-pregnant uterus, so the compulsory properties of the uterus can be seen in the human body.

Effect of the extract treatment of Example 1 on the contraction frequency and contractility of the pregnant uterus Test group Shrinkage (%) Contraction force (%) Extract of Control Example 1 1 ug / ml Extract of Example 1 10 ug / ml Extract of Example 1 100 ug / ml Extract of Example 1 300 ug / ml Oxytocin 0.1 mU / mL Oxytocin 1 mU / mL Oxytocin 10 mU / Ml 100.0 ± 0.0111.0 ± 4.32109.6 ± 5.55112.4 ± 5.54111.1 ± 7.17133.9 ± 3.21 ^** 217.3 ± 4.75 ^** 249.0 ± 1.92 ^** 100.0 ± 0.0115.3 ± 3.44157.2 ± 6.11 ^* 172.8 ± 6.26 ^** 231.7 ± 4.71 ^** 155.3 ± 1.96 ^** 205.9 ± 2.48 ^** 249.6 ± 2.06 ^**

Values expressed in ± are mean values obtained from 3-5 animals.

^* (P <0.05), ^** (P <0.001): significantly different from control group.

From the results of the above experiment, the extract according to Example 1 of the present invention is stable in the number of shrinkages compared to the control group, so that it does not give the fetus or the mother a stable and strengthens the contraction force of the oxytocin hormone by strengthening the natural labor It showed contractile force and approximate strength, whereas oxytocin hormone was found to have a very high resin that amplified the risk of excessive bleeding and fetal death due to too many contractions.

* Toxicity test: Three doses of 1 g / kg and 2 g / kg alc 5 g / kg, respectively, in male and female rats of the Sprague-Dawley strain to investigate the presence or absence of toxicity by oral administration to the rats of the inventive extract of the test substance. Five oral single doses were administered and 14 deaths, general transpiration, weight change and autopsy findings were observed. The result of this test is as follows.

(1) Death during the test period Animals did not die in all the administration groups of the extract extract extract during the test period, and there was no change in general symptoms observed compared to the control group.

(2) There was no significant difference in body weight change in all the administration groups after the invention extract administration compared to the control group.

(3) In the gross autopsy findings, no abnormalities were observed in all the animals to which the invention extract was administered.

As a result, no clinical symptoms were observed in all test substance-treated groups compared to the death and control groups. In addition, no specific symptoms were observed in the anatomical findings at weight change and autopsy. That is, the herbal extract according to the present invention did not show toxicity at doses of 5 g / kg or less. Therefore, the extract of the present invention by oral administration is determined that the LD ₁₀ (lethal dose) in the rat is more than 5g / kg.

Hereinafter, examples of various formulations of the extracted herbal composition according to the present invention will be described.

Preparation Example 1

56.25 g of Angelica and 37.5 g of Cheongung were finely pulverized and mixed, and extracted with an extract such as hot water and alcohol. The resulting extract was cooled and filtered and then prepared for tablet and liquid oral administration.

<Formulation Example 2>

75 g of antler and 37.5 g of uterus were finely ground and mixed, and extracted with an extract such as hot water and alcohol. The resulting extract was cooled, filtered and prepared for tablet and liquid oral administration.

Preparation Example 3

75 g of antler and 56.25 g of Angelica pulverized were finely ground and mixed, and extracted with an extract such as hot water and alcohol. The resulting extract was cooled, filtered and prepared for tablet liquid oral administration.

Preparation Example 4

75 g of antler, 56.25 g of Angelica, and 37.5 g of Cheongung were finely pulverized and mixed, followed by extraction with an extract such as hot water and alcohol. The resulting extract was cooled, filtered and prepared for tablet and liquid oral administration.

Preparation Example 5

75 g of antler was finely ground and extracted with an extract such as hot water and alcohol. The resulting extract was cooled, filtered and prepared for tablet and liquid oral administration.

The present invention as described above can help promote the delivery of herbal medicines obtained from deer antler, Angelica, horoscopes, etc. during delivery, stable and fast through the uterine contraction while replacing the function of the previously used oxytocin hormone It can be used as a food for inducing labor. This is expected to be a new food that eliminates side effects such as carcinogenicity of the conventional hormone preparations, death of the fetus, and excessive uterine bleeding in pregnant women.

In addition, oxytocin hormone is compulsory and has many side effects by contracting action on both the uterus of pregnant and non-pregnant women, whereas the composition of the present invention does not work on non-pregnant women and selectively works only on the uterus and childbirth of pregnant women. Able to know.

Although the present invention has been described above according to an embodiment of the present invention, it will be apparent to those skilled in the art that various modifications may be made without departing from the spirit of the present invention.

Claims (17)

A health food for pregnant women, comprising antler, angelica, and uterus in a weight ratio of 0.8 to 4: 0.5 to 4: 1, and having a formulation of powder, lactose or pill. delete delete delete delete delete delete delete delete delete Mixing 168.75 g of a herbal mixture comprising antler, Angelica and scorpio in a weight ratio of 0.8-4: 0.5-4: 1 and 1.5 L of water; Heating the herbal mixture and the mixture of water to a temperature above the boiling point of the water; Concentrating by heating in a temperature range of the boiling point of water ± 10 ℃; Cooling the obtained concentrate; And The method for producing a health food for pregnant women comprising the step of preparing the obtained cooling liquid as a powder, a medicated liquid, or a pill. delete 12. The method of claim 11, wherein the obtained cooling liquid is dried to prepare a water-soluble granule. The method of claim 11, further comprising the step of freeze-drying the coolant. Preparing a medicinal herb mixture by mixing antler, angelica and scorpio in a weight ratio of 0.8 to 4: 0.5 to 4: 1; And Method of producing a health food for pregnant women comprising the step of preparing a powder by grinding the prepared herbal mixture. The method of claim 15, further comprising the step of mixing the powder and 15 to 20 parts by weight of white powder or syrup of the powder to prepare a pill. delete