KR100464260B1 - PRIMER SET SPECIFIC TO GENUS STREPTOMYCES, rpoB GENE SEQUNCE, AND IDENTIFICATION METHOD OF GENUS STREPTOMYCES BY USING THE SAME - Google Patents

Abstract

The present invention relates to PCR primers specific for rpoB gene of Streptomyces strain, polynucleotides representing 306-bp fragment of RNA polymerase gene ( rpoB ) that can be used for the identification of Streptomyces strain, and Streptomyces genus using them A method for identifying strains. The identification method of the present invention has the advantage of providing a simple, economical and accurate identification method by solving the problem of slow growth rate and the presence of various species, and the problems of material-oriented identification and 16s rDNA identification. It can be widely used to identify Streptomyces strains.

Description

Streptomyces strain-specific primers, gene gene segments, and methods for identifying Streptomyces genus strains using the same

[Technical Field]

The present invention relates to the identification of Streptomyces specific primers and Streptomyces. More specifically, the rpoB gene segment and a method for detecting or identifying Streptomyces sp. Strain using the same.

[Background]

Since the discovery of streptomycin, many researchers have studied Streptomyces spp. As the subject of antibiotic-producing strains. As a result, many antibiotics have been found. Then Streptomyces as various physiologically active substances are still separate addition to the antibiotics from the MRS in strain has been progress in the new antibiotic search for Streptomyces MRS spp, Streptococcus MRS spp Alcohol (Actinomycetes), including Streptomyces Mrs in the antibiotic As a microorganism, it was treated as an industrially and medically important microorganism. The research of these antibiotics is progressing from searching for a substance that is effective for bacterial or fungal infections to a substance that is effective for cancer or viral diseases, and recently, research on new antibiotics or antibiotic derivatives that are effective for resistant bacteria, gram-negative bacillus, and anticancer antibiotics. Until now, the situation is active. Recently, due to the frequent appearance of resistant bacteria against antibiotics, the search for a compound having a strong antimicrobial activity selectively against a specific pathogen is preferred. In recent years, research on the production of antibiotics by recombination has also been conducted (Bachmann BO, Li R, Townsend CA. beta-Lactam synthetase: a new biosynthetic enzyme.Proc Natl Acad Sci US A. 1998; 95 (16): 9082 -6).

Streptomyces spp. Strains are the most diverse, as well as having different physiological metabolic capacity within the same species (Anderson AS, Wellington EM.The taxonomy of Streptomyces and related genera.Int J.) Syst Evol Microbiol. 2001 51 (3): 797-814.). So far, about 2/3 of the 100,000 bioactive substances detected from microorganisms are found in Streptomyces strains, so the strains of the Streptomyces strain are very large in the search for various bioactive substances. It is emerging as one of the most important microorganisms in the biological material industry. Therefore, Streptomyces strains can be used in agricultural and fishery industry (breeding, pest control, etc.), environmental industry (waste decomposition treatment), fine chemical industry (industrial chemicals), food industry (raw materials, additives, etc.), semiconductor industry (biosensor), and medicine. There is also great potential to be used in industry. In recent years, studies on using natural products for the purpose of preventing, alleviating or treating various diseases have been actively conducted (Emmert EA, Handelsman J. Biocontrol of plant disease: a (gram-) positive perspective.FEMS Microbiol Lett. 1999 1; 171 (1): 1-9; Nielsen J. Metabolic engineering: techniques for analysis of targets for genetic manipulations. Biotechnol Bioeng. 1998; 58 (2-3): 125-32; .Hutchinson C, Colombo A. Genetic engineering of doxorubicin production in Streptomyces peucetius : J Ind Microbiol Biotechnol. 1999 Jul; 23 (1): 647-652)

Therefore, the investigation of antibiotics using Streptomyces spp. And Streptomyces spp. In the situation where the pollution of the natural environment is getting serious, it is urgent to secure and conserve microbial resources in Korea. Therefore, not only the specific bioactive substances produced by microorganisms, but also the precise phylogenetic location or classification of the microorganisms is important.

Streptomyces strains have been the subject of novel substance screening as a report of natural organic compounds, and much research has been done on the biosynthetic mechanism of metabolites. Therefore, the classification of Streptomyces spp. Has gained considerable importance in the present because of the search for new substances by the identification and identification of new bacteria. Screening of new substances by current strains of Streptomyces is highly material-specific, which often overlaps with existing strain-producing strains. Therefore, this new material search method is likely to obtain a variety of new materials if the new species or strains have been determined by the identification of the strain of Streptomyces genus.

Currently, the classification of Streptomyces genus strains is based on numerical taxonomy by physiological, morphological or biochemical properties by existing phenotypic properties. Streptomyces genus has the disadvantage that it is difficult to classify the species by biochemical or physiological methods because it is the most diverse type of bacteria and relatively slow development rate compared to other microorganisms (Skerman, VBD, McGowan, V.). , Sneath, PHA (ed): Approved Lists of Bacterial Names.Int. J. Syst. Bacteriol. 30: 225-420 (1980)).

The classification method based on the characteristics of traits is a reliable method for determining the species of Streptomyces strains because of the large number of Streptomyces strains and the slow growth rate (E. coli cell division cycle: 20 minutes, Streptomyces division cycle: 2-3 hours). There is a problem with time and with accuracy. The trend is to identify chronometer molecules that represent the systematic relationships of all bacteria with sequencing methods and identify molecular taxonomy to determine species. The bacterium identification method by sequencing 16S rDNA is most widely used as a target of a kit for detecting pathogens and other important bacteria by molecular biological methods (Genprobe kit for classifying mycobacteria).

Molecular taxonomy using 16S rDNA and comparative sequencing of several different targets is difficult to accurately classify due to various disadvantages of the target gene, cost and time issues (Ueda). K, Seki T, Kudo T, Yoshida T, Kataoka M. Two distinct mechanisms cause heterogeneity of 16S rRNA.J Bacteriol. 1999 Jan; 181 (1): 78-82). First, in order to identify bacteria by comparative sequencing method by sequencing method, although there are hypervariable regions with severe sequencing, the entire 1.5 kbp sequence must be analyzed for accurate bacterial identification. There is a big problem in terms of time and cost as well. This drawback has the problem of planting many oligonucleotides in the future development of identification methods by DNA chips. Secondly, it is costly to analyze many data of more than 450 species, such as the genus Streptomyces. Thus, while a database of 16S rDNA of other species is currently clearly established on Genbank, Streptomyces strains are not yet fully established 16s rDNA database of the entire species except for some species. Third, the most deadly weakness of the classification method by 16S rDNA is that in some species, they exist as multi-copy genes on the whole chromosome and also have different allele sequences. It has been reported to exist as a nucleotide sequence (Ueda K, Seki T, Kudo T, Yoshida T, Kataoka M. Two distinct mechanisms cause heterogeneity of 16S rRNA. J Bacteriol. 1999 Jan; 181 (1): 78-82).

In addition, there are several sequences of 16S rDNA in one strain, which poses a significant technical problem in sequencing. In other words, 16s rDNA, a target gene of Streptomyces spp., Was amplified by polymerase chain reaction, and this product was not directly sequenced. Cloning into a vector was required to prepare several clones and to analyze their base sequences. There is a problem.

Therefore, in addition to 16S rDNA, it is necessary to select an alternative clock molecule to be used for the identification of Streptomyces spp., And to establish a method for identifying Streptomyces spp.

In order to solve the above problems, the present invention is to provide a primer specific for rpoB gene of Streptomyces sp.

Another object of the present invention is to provide an rpoB gene segment that can be used for the identification of Streptomyces sp.

Another object of the present invention is to provide a method for identifying Streptomyces spp. Using the rpoB segment of the Streptomyces spp.

Figure 1 shows a schematic diagram created by taking the 306-bp site used as a target of the present invention among rpoB nucleotide sequences of various bacteria conventionally analyzed.

FIG. 2 is a diagram illustrating the rpoB sequences of Streptomyces strain specific rpoB primers and S. coelicolor , M. smegmatis , M. tuberculosis , and M. leprae 4 strains.

Figure 3 is an electrophoresis picture of the 352-bp rpoB fragment of the standard strain amplified with Streptomyces specific rpoB primer according to the present invention.

Figure 4 shows the nucleotide sequence homology or homogeneity (similarity value) results of the streptomyces 24 weeks.

Figure 5 shows the 306-bp rpoB gene segment phylogeny of Streptomyces standard strain 102 strain.

Figure 6 shows the results of identification at the species level of non-standard strains of 8 weeks by comparative base sequence analysis using rpoB 306-bp segment, Figure 6a is an example of identification of S. olivichromogenes 1 week, Figure 6b is S. peucetius 2 weeks 6c shows an identification of 3 strains of S. hydroscopicus , and FIG. 6d shows an identification of 2 strains of S. albus .

The present invention relates to an effective detection or identification method of Streptomyces strain using sequence information of the rpoB gene, Streptomyces strain rpoB segment and rpoB segment of Streptomyces strain.

In view of the problems in the identification and classification of Streptomyces sp. Strains, the present inventors have provided primer pairs used for PCR amplification of all Streptomyces sp. A database was constructed by analyzing the nucleotide sequence of the gene fragment (306-bp) of the alternative target gene RNA polymerase ( rpoB ). In addition, by amplifying the rpoB fragment of the target strain, and compared with the database to develop a new Streptomyces strain detection or identification method.

The present invention relates to polynucleotides representing rpoB segments of Streptomyces strains for detecting or identifying Streptomyces strains, or fragments thereof.

As a replacement molecule capable of substituting 16S rDNA for classifying Streptomyces strains, in the present invention, a 306-bp rpoB gene segment encoding RNA polymerase subunit B was used. In order for a gene to be a clock molecule that reflects the systematic relationship of a bacterium, certain requirements must be met.

First, target genes must be functionally necessary and conserved in all bacteria. Since 16S rDNA, a representative clock molecule, plays an essential role in protein synthesis, every bacterium has this gene, and the genetic variation between each bacterium shows an evolutionary time relationship. The rpoB gene used as a target in the present invention also satisfies these conditions because it is an essential enzyme involved in the transcription of bacteria. Second, the genetic variation of the target gene should only be caused by temporal factors that reflect evolution. In other words, the nucleotide sequence does not occur by lateral transfer due to selection pressure between species. The rpoB gene, which is used as a target of this study, does not cause sequence variation between species. Third, the target gene should show the conservation of the species within the genus, such as interspecies variation, which is appropriate for showing the phylogenetic relationship. The rpoB gene has been described in several articles (Kim BJ, Lee SH, Lyu MA, Kim SJ, Bai GH, Chae GT, Kim EC, Cha CY, Kook YH. Identification of mycobacterial species by comparative sequenceanalysis of the RNA polymerase gene ( rpoB ) .J Clin Microbiol 1999 Jun; 37 (6): 1714-20; Lee SH, Kim BJ, Kim JH, Park KH, Kim SJ, Kook Y H. Differentiation of Borrelia burgdorferi sensu lato on the basis of RNA polymerase gene (rpoB) sequences. J Clin Microbiol. 2000 Jul; 38 (7): 2557-62.

The 306-bp rpoB segment is the most conserved amino acid and sequence among the various eubacteria among the amino acids constituting the RNA polymerase subunit, and is known as the most important site in the enzyme function of the total RNA polymerase. We also aim to target 306-bp sites associated with rifampin resistance in Mycobacterium tuberculosis and E. coli (Telenti, A., P. Imboden, F. Marchesi, D. Lowrie, S. Cole, MJ Colston, L. Matter, K. Schopfer, and T. Bodmer. 1993. Detection of rifampin-resistance mutations in Mycobacterium tuberculosis.Lancet 341: 647-650). This site is also surrounded by highly conserved regions 5 and 6 (HCR5, HCR6), where amino acids are preserved among all eubacteria. For this reason, the inferred sequences of this region enable the preparation of Streptomyces strain specific primer pairs (Boor, KJ, ML Dunkan, and CW Price. 1995. Genetic and transcriptional organization of the region encoding the subunit of Bacillus subtilis RNA polymerase. J. Biol. Chem. 270: 20329-20336).

In order for a site to be a suitable target for identification and diagnosis, it must be examined whether it is a clock molecule that shows a systematic relationship between bacteria. Therefore, in order to find out whether the rpoB 306-bp region of the present invention is appropriate as a clock molecule, a 306-bp region used as a target of the present invention is generated from the rpoB sequences of various bacteria whose base sequences have been analyzed for other purposes. (FIG. 1). As shown in Figure 1, it can be seen that the existing bacteria are clearly divided into gram (-), gram (+), and primitive groups. This fact shows that this rpoB 306-bp region is a clock molecule that can be used to identify bacteria.

Compared to 16s rDNA, which is widely used as a conventional clock molecule, the rpo B 306-bp segment of the present invention has the following advantages.

(i) In order to accurately identify the target by 16S rDNA by comparative sequencing method, the whole gene of about 1.5 kbp should be analyzed. But,rpoBGenes can be accurately identified by only 306-bp sequencing. In classifying Streptomyces strainsrpoBThe utility of gene segments can be inferred indirectly from successful experiments in Streptomyces spp. And Mycobacterium spp. This difference has the advantage that the cost of identification can be saved several times.

(ii) The biggest disadvantage of the Streptomyces strain identification method using 16S rDNA is the direct sequencing of 16s rDNA, because 16s rDNA exists in several species and the nucleotide sequence of each copy is different in some species. It can't be done in a way. In other words, after cloning, multiple clones must be sequenced, which requires several times more labor, time, and cost than direct sequencing. However, since only one operon was found in the rpoB gene of the Streptomyces genus strain, the rpoB gene sequence of all species can be directly analyzed by sequencing method.

(iii) 16S rDNA has a gap in nucleotide sequence due to different nucleotide sequence lengths of hypermutated regions. However, the rpoB segments to be used as targets in this study do not have gaps and all have the same size (306-bp) sequences. Therefore, this property is a significant advantage in determining the sequence sequence or sequence.

(iv) 16S rDNA has been performed by the base sequence database since the mid-1980s, and the sequencing of the entire Streptomyces strain has not been analyzed yet, sporadicly by different researchers. These sequences are often made when the method is not technically complete. Therefore, the obtained 16s rDNA database has many problems. However, since the rpoB sequences of the Streptomyces sp. Strain of the present invention are entirely new on Genbank, a unique database for classifying Streptomyces sp. Strains can be constructed.

The numbers shown in FIG. 2 represent the amino acid sequence of the subunits of RNA polymerase of Streptomyces ceolicolo. The amplification products of the polymerase chain reaction were 352-bp in total, ranging from the second base of the 266 amino acid codon to the second base of the 383 amino acid codon. Sequencing was performed on a total of 306-bp from the second base of the 274th amino acid codon to the first base of the amino acid 376 codon. A total of 69-bp of amino acids 332 to 354 represents the RIFR site that causes rifampin resistance in Mycobacterium tuberculosis. This sequencing site shows that the site contains rifampin resistance in Mycobacterium tuberculosis. An asterisk (*) indicates a hot spot site where sequence variations occur frequently in Mycobacterium tuberculosis.

According to the present invention, the target strain can be identified using the 306-bp fragment sequence difference of the rpoB gene of the Streptomyces genus strain. For example, all of the molecular biological methods using the difference of these sequences can be applied to the present invention to detect or identify Streptomyces strains by comparing the r6-B gene sequence of the 306-bp segment of the standard strain with the target strain. For example, rpoB sequencing, hybridization method using a 306-bp segment or fragment thereof of a Streptomyces strain as a probe, or a method comprising the rpoB gene or fragment thereof of the Streptomyces strain It can also be attached to the microarray and analyzed by reacting the rpoB gene amplification product of the sample strain.

In one example of the present invention, one of the Streptomyces genus strains because of the most diverse species of the Streptomyces genus standard strain of 161 and the rare strain Streptomyces genus micro strains of the highest strain among the standard strain of one species After amplifying a total of 162 types of rpoB fragments, the nucleotide sequences of each species were analyzed by direct sequencing. A database is constructed by performing a multi-alignment of the nucleotide sequences of the completed 162 Streptomyces genus strains, and completes the schematic by using them. The numerical counts analyzed by biochemical and physiological methods are compared with the rpoB schematics completed in this experiment.

Streptomyces strain standard strain using Streptomyces strain specific primers For the amplification of 352-bp rpoB DNA segment of strain 162 strains, the strains shown in Tables 1a to 1b were selected as standard strains. Nonstandard strains are shown in Table 2. These strains were obtained from the Genetic Bank of Biotechnology Research Institute, located at 52, Eun-dong, Yuseong-gu, Daejeon, Korea.

No. Strain name source No. Strain name source One K. azatica KCTC 9699 41 S. cinereoruber KCTC 9707 2 K. cystarginea KCTC 9746 42 S.cinereus KCTC 9066 3 K. griseola KCTC 9671 43 S. cinnamonensis KCTC 9708 4 K. mediocidica KCTC 9733 44 S. cirratus KCTC 9709 5 K. phosalacinea KCTC 9792 45 S. clavuligerus KCTC 9095 6 K. setae KCTC 9793 46 S. coelicolor KCTC 9005 7 M. echinospora KCTC 9549 47 S. coeruleorubidus KCTC 1743 8 S. abikoensis KCTC 9662 48 S. collinus KCTC 9713 9 S. achromogenes KCTC 1740 49 S. corchorusii KCTC 9715 10 S. acrimycini KCTC 9679 50 S. crystallinus KCTC 9717 11 S. actuosus KCTC 9112 51 S. cuspidosporus KCTC 9718 12 S. aculeolatus KCTC 9680 52 S. cyaneus KCTC 9719 13 S. alanosinicus KCTC 9683 53 S. diasticus KCTC 9142 14 S. albireticuli KCTC 9685 54 S. djakartensis KCTC 9722 15 S. albofaciens KCTC 9747 55 S. durhamensis KCTC 9723 16 S. alboflavus KCTC 9674 56 S. echinoruber KCTC 9725 17 S. albogriseolus KCTC 9773 57 S. ederensis KCTC 9726 18 S. albolongus KCTC 9676 58 S. ehimensis KCTC 9728 19 S. alboniger KCTC 9014 59 S. flaveolus KCTC 9737 20 S. albosporeus KCTC 9666 60 S. flavofuscus KCTC 9760 21 S. alboviridis KCTC 9750 61 S. fradiae KCTC 1919 22 S. albulus KCTC 9668 62 S. galilaeus KCTC 9026 23 S. albus KCTC 1082 63 S. globisporus KCTC 9027 24 S. almquistii KCTC 9751 64 S. griseochromogenes KCTC 9028 25 S. aminophilus KCTC 9673 65 S. griseolus KCTC 9780 26 S. antimycoticus KCTC 9694 66 S. griseoviridis KCTC 9080 27 S. argenteolus KCTC 9695 67 S. griseus KCTC 9781 28 S. armeniacus KCTC 9120 68 S. hiroshimensis KCTC 9782 29 S. avidinii KCTC 9757 69 S. hygroscopicus KCTC 9113 30 S. bacillaris KCTC 9018 70 S. libani libani KCTC 9033 31 S. bambergiensis KCTC 9019 71 S. limosus KCTC 1868 32 S. bikiniensis KCTC 9172 72 S. lincolnensis KCTC 9022 33 S. cacoi asoensis KCTC 9700 73 S. longwoodensis KCTC 9783 34 S. capillispiralis KCTC 1719 74 S. melanogenes KCTC 9205 35 S.carpinensis KCTC 9128 75 S. minutiscleroticus KCTC 9123 36 S.catenulae KCTC 9223 76 S. nitrosporeus KCTC 9761 37 S.celluloflavus KCTC 9702 77 S. noboritoensis KCTC 9060 38 S.chartreusis KCTC 9704 78 S. nodosus KCTC 9035 39 S.chattanoogensis KCTC 1087 79 S. nojiriensis KCTC 9784 40 S.chrysomallus KCTC 9705 80 S. olivaceoviridis KCTC 9132

No. Strain name source No. Strain name source 81 S. olivochromogenes KCTC 9064 122 S.amakusaensis KCTC 9753 82 S. pactum KCTC 9165 123 S.aburaviensis KCTC 9663 83 S. paradoxus KCTC 9118 124 S.albospinus KCTC 9762 84 S. peucetius KCTC 9199 125 S.albovinaceous KCCM 40177 85 S. phaeochromogenes KCTC 9763 126 S.anabdii KCTC 9687 86 S. plicatus KCTC 9040 127 S.antibioticus KCTC 1137 87 S. pulveraceus KCTC 9766 128 S.atroolvaceous KCTC 9017 88 S. rameus KCTC 9767 129 S.aureufaciens KCCM 40127 89 S. rimosus KCTC 1077 130 S.azureus KCCM 40485 90 S. roseosporus KCTC 9568 131 S.baldacii KCCM 41326 91 S. sclerotialus KCTC 9065 132 S. candidus KCTC 9020 92 S. setonii KCTC 9144 133 S. caseius KCCM 40740 93 S. siovaensis KCTC 9043 134 S. californicus KCCM 40605 94 S. somaliensis KCTC 9044 135 S. carpinensis KCTC 9128 95 S. spectabilis KCTC 9218 136 S. chromogenes KCCM 40727 96 S. subrutilus KCTC 9045 137 S. cinnamoneus KCCM 40572 97 S. tubercidicus KCTC 9109 138 S. citreofluorescens KCTC 9710 98 S. vinaceus KCTC 9771 139 S. coerulescens KCCM 40508 99 S. violarus KCTC 9788 140 S. coeruleofuscus KCCM 40506 100 S. violascens KCTC 9785 141 S. coralus KCCM 40642 101 S. virginiae KCTC 1747 142 S. cremeus KCCM 40509 102 S. xantophaeus KCTC 9220 143 S. cyaneofuscatus KCCM 40517 103 S. albaduncus KCTC 1741 144 S. disatochromogenes KCCM 40449 104 S. althioticus KCTC 9752 145 S. erumpens KCTC 9729 105 S. ambofaciens KCCM 40182 146 S. erythraeus KCCM 40477 106 S. anulatus KCCM 40190 147 S. eurythermus KCTC 9731 107 S. anthocyanicus KCTC 9755 148 S. fimbriatus KCCM 11888 108 S. cellulose KCTC 9703 149 S. flavotricini KCCM 40520 109 S. chivaensis KCTC 9786 150 S. flavovirens KCCM 40165 110 S. coelescens KCCM 40742 151 S. fulvissimus KCTC 9773 111 S. griseoflavus KCCM 12624 152 S. fumanus KCCM 40522 112 S. humiferus KCTC 9116 153 S. gougeroti KCCM 40681 113 S. lividans KCTC 1154 154 S. griseoruber KCCM 40658 114 S. murinus KCTC 9492 155 S. griseolosporeus KCTC 9791 115 S. pilosus KCCM 40480 156 S. griseostramineus KCCM 40526 116 S. rubiginosus KCTC 9042 157 S. hachijoense KCCM 32306 117 S. tendae KCCM 40105 158 S. halstedii KCCM 40613 118 S. umbrinus KCCM 40316 159 S. humidus KCCM 40647 119 S. violaceoruber KCTC 9787 160 S. indigoferus KCCM 40495 120 S. xanthocidicus KCCM 40286 161 S. kifunensis KCTC 9734 121 S. yokosukanens KCCM 40633 162 S. kurssanovi KCCM 40527

No. Strain name source No. Strain name source One S. olivichromogenes KCTC 9090 5 S. hydroscopicus KCTC 9031 2 S. peucetius KCTC 9038 6 S. hydroscopicus KCTC 9069 3 S. peucetius KCTC 9242 7 S. albus KCTC 1136 4 S. hydroscopicus KCTC 9030 8 S. albus KCTC 1533

In the present invention, all 162 strains used when constructing the database use only the strains designated as standard strains by the Korea Biotechnology Research Institute Gene Bank (KCTC), and all the strains shown in the examples are strains that are different subtypes of the standard strains (not the standard strains). ) Shows that this database is suitable for identification of species.

Polymerase chain reaction using primers capable of amplifying the selected Streptomyces genus resulted in 1% agarose gel electrophoresis to produce 352-bp rpoB gene segments in all 162 standard strains. (FIG. 2). The base sequence of the obtained 306-bp rpoB fragment is represented by SEQ ID NOs: 3 to 104 in the sequence listing. The nucleotide sequence database of 162 strains of Streptomyces spp. Analyzed in the present invention was found to be all new nucleotide sequences on Genbank search .

As a result of comparing the nucleotide sequences of each other by multialigning the analyzed nucleotide sequences, the first 162 strains of the standard strains all have different nucleotide sequences. That is, it shows the interspecies variation between each species. Genes targeted for identification should be preceded by the diversity of sequences between each species in order to be used for the identification of species. Second, all of the 162 nucleotide sequences encode 306-bp bases without polynucleotide insertion or deletion on polysequence. That is, there is no gap on the polyalignment 16S rDNA has a high frequency gap on the alignment. When sorting, gaps are generally known to be prone to error in constructing the entire phylogenetic tree because they tend to analyze all sorted genes corresponding to the site. Therefore, the superiority of the rpoB gene used in the present invention can be confirmed.

Neighbor-Joining phylogenies were constructed using Mega software as described above. When analyzing the phylogenetic tree, it was found that all 102 strains showed different sequences and 162 unique segments. In addition, 161 strains of Streptomyces spp.M. echinosporaIt can be seen that forming one group compared to (FIG. 5). In addition, a comparison of the Streptomyces group of 161 states showed that Kitasatospora, which is currently controversial in classification (Kitasatospora)And streptofertisilium (Streptoverticillium)Group Streptomyces It was confirmed that they formed a unique small group within the group. These two groups are classified into different genera because they have significantly different physiological and biochemical properties from streptomyces, such as containing large amounts of meso-DPA (diaminopimelic acid) in cells, but they are classified into different genera, such as 16S rDNA. In terms of molecular taxonomy, it is not just an independent genusStreptomyces(Zhang Z, Wang Y, Ruan: A proposal to revive the genus Kitasatospora (Omura, Takahashi, Iwai, and Tanaka 1982, Int J Syst Bacteriol. 1997; 47 (4): 1048) -54).

Comparing the phylogenetic tree using the 306-bp rpoB target gene of the present invention shows a result similar to the fact by conventional molecular biological classification. That is, the six Kitasatospora strains ( K. azatica, K. crystarginea, K. griseola, K. mediocidica, K. phosalcinea, K. setae ) and three Streptoferticesium strains ( S. abikoensis ) used in the present invention. , S. albirecticuli, S. ehimensis ) belong to the small group of the entire Streptomyces group, but do not form an independent segment like M. echinospora (FIG. 5).

Thus, the 306-bp rpoB gene segment used in the present invention can be said to be a good chronometer molecule that well reflects the phylogenetic relationship in Streptomyces spp. In view of the favorable identification of clock molecules reflecting systematic relationships, the 306-bp rpoB gene segment could be successfully applied to the classification of Streptomyces genus.

After sequence alignment, the sequence homogeneity of the 162 strain standard strain was examined as described above. As a result, all of the 162 strains showed different sequencing. The sequence homogeneity ranged from 99.7% (sequence between S. acrimycini and S. albogriseolus ) to 84.6% (sequence between M. echinospora and S. lincolensis ) (Figure 4). Comparing the nucleotide sequence and homogeneity of M. echinospora strains of the genus Streptomyces genus of 101 strains, all showed a homogeneity of less than 90% (showing up to 84.6-88.9%) (Fig. 4). In FIG. 4, 24 species of Streptomyces spp. Were selected and selected, but the strains were selected and displayed so that all values from the smallest to the highest were entered.

Thus , Streptomyces spp. Are the rarest Streptomyces spp. That are closest in phylogenetic relationship. It can be seen that the sequence of heterogeneity of 10% or more with the micromonospora. Comparing the nucleotide sequence homogeneity of 16S rDNA, both micromonospora show more than 95% sequence homology with Streptomyces, so the 306-bp rpoB gene segment used in this patent was identified at the genus level of the genus Streptomyces strain. It can be seen that there is an advantage.

Comparing the sequence homogeneity of the standard strains of Streptomyces 101 strains excluding M. echinospora strains, 99.7% to 88.9% (sequence of S. armeniacus and S. lincolensis ). It can be confirmed that homogeneity is shown. Therefore, it can be seen that the sequence heterogeneity of 0.3-11.1%. It was confirmed that interspecies variation, which is one of the most important characteristics of the target gene for identification, was much higher than 16S rDNA having no sequence heterogeneity between streptomyces.

The present invention relates to a primer specific for a strain of the genus Streptomyces strain for amplification of the rpoB gene segment of the Streptomyces genus strain. The primer is preferably a nucleotide comprising the nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 2.

Preparation of primers capable of amplifying rpoB gene segments specific for Streptomyces strain, compared to the rpoB base sequence of Mycobacterium spp. It is selected as a forward and reverse primer. Then, a primer is prepared such that the site amplified by the primer pair includes a site associated with rifampin resistance of E. coli and Mycobacterium tuberculosis. Subsequently, the polymerase chain reaction using primers prepared from 162 DNA of Streptomyces spp strains to be analyzed in this experiment can amplify 352-bp rpoB fragments of all Streptomyces spp strains. Analyze whether or not.

To prepare primers capable of amplifying all Streptomyces strains, Streptomyces coelicolor ( S. coelicolor (Genbank No. AL160431)), Mycobacterium smegmatis ( M ), which analyzed the entire rpoB sequence in Genbank. smegmatis (Genbank No. U24494)), Mycobacterium tuberculosis ( M. tuberculosis (Genbank No. L27989)), Mycobacterium leprae ( M. leprae (Genbank No. Z14314)) 4 weeks rpoB Compare sequences to each other. The resulting forward primer sites, although belonging to different genera, show a 100% homology with 4 weeks of sequence, Streptomyces. 20 base sequences from the 2nd base of amino acid 266 codon to the 3rd base of amino acid 272 codon based on the Copolymeric RNA polymerase subunit (5'-TC GAC CAC TTC GGC AAC CGC-3 ') The site corresponding to can be selected and named SRPOF1 (FIG. 2). The reverse primer site is mycobacteria belonging to slow growing mycobacteria though. Tuberculosis and mycobacteria, a mitobacterium belonging to the rapidly growing mycobacteria, although they differ by one base from leprae. 20 nucleotide sequences showing 100% homology with smegmatis can be selected (FIG. 2). The nucleotide sequences from the second base of amino acid 383 codon to the first base of codon of amino acid 377 in reverse direction. SRPOR1 (5'- TC GAT CGG GCA CAT GCG GCC-3 '). Streptomyces strains are more closely related to rapid growth than slow growth compared to Mycobacterium spp. In systematic or physiological characteristics. Therefore, according to the present invention, the SRPOF1 and SRPOR1 primer pairs capable of amplifying the entire Streptomyces genus strain may be referred to as primers that are systematically highly calculated.

The present invention relates to a method for detecting or identifying a Streptomyces strain using the rpoB gene segment consisting of the following steps:

(1) amplifying the rpoB gene segment of the desired strain using primers that specifically amplify the rpoB segment of the Streptomyces strain of the Streptomyces strain

(2) analyzing the nucleotide sequence of the rpoB gene segment,

(3) Compare the nucleotide sequence of step (2) with the nucleotide sequence of the rpoB gene segment of the standard strain.

In the step (1), the primer pair used for the detection or identification of Streptomyces strain includes all the primers capable of amplifying the rpoB gene of the Streptomyces strain, in particular the nucleotide sequence shown in SEQ ID NO: 1 or 2 Polynucleotides are preferred.

Using the primers specific for the Streptomyces genus strain, the rpoB gene segment of the target strain is amplified by PCR technique to analyze the nucleotide sequence. PCR amplification and sequencing can use any method known to those skilled in the art (Kim BJ, Lee SH, Lyu MA, Kim SJ, Bai GH, Chae GT, Kim EC, Cha CY, Kook YH. Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol. 1999 Jun; 37 (6): 1714-20.)

The database of the rpoB segment of the standard strain is a nucleotide sequence represented by SEQ ID NO: 3 to SEQ ID NO: 164 in the sequence listing.

After analyzing the rpoB sequences of the standard strain and the target strain, and comparing them with each other, to determine that the strain is the strain, the sequence of the bacteria to be identified in the sorted database is analyzed, added to the database, and the sequencing is performed again. 162 nucleotide sequence database + nucleotide sequence of the bacteria to be analyzed) After completing the phylogeny, branches can be formed close to the corresponding species, and the species can be determined by the phylogeny. In addition, if it is determined by sequence homology, it is also possible to identify species which show homology of 99.7% or more. This is because the nucleotide sequence variation between all species is more than 0.3% and the sequence homology between each species is more than 99.7%.

Streptomyces strain constructed in this studyrpoBIn order to verify that the database can be applied to the classification, a total of eight strains of three non-standard strainsrpoBThe base sequence is analyzed and identified by comparative base sequence analysis. In order to confirm that the completed 162 strain Streptomyces strain standard strain database can be actually applied to the identification of bacteria, the non-standard strain Streptomyces.S. olivichromogenes) Week 1 (KCTC 9090), Streptomyces. 2 weeks (KCTC 9038, KCTC 9242), Streptomyces hydroscopicus 3 weeks (KCTC 9030, KCTC 9031, KCTC 9069),And Streptomyces albus 2 weeks (KCTC 1136, KCTC 1533) A total of four strains of 8 strains were identified by comparative sequencing method.

As a result, non-standard strain Streptomyces. Olivier Cromogenes (KCTC9090) One strain of Streptomyces is 100% sequence homology and exactly the standard strain on the phylogenetic tree. Olivier Cromogenes It could be confirmed that (KCTC9064) is located (Fig. 6A). Non-standard strain Streptomyces fusethius Two weeks (KCTC 9038, KCTC 9242) showed 100% and 99.7% sequence homogeneity, respectively,S. peucetius(KCTC 9199) was found to be located (Fig. 6B). Nonstandard strainS. hydroscopicus3 weeks (K CTC 9030, KCTC 9031 and KCTC 9069) show 100%, 99.7%, and 997% sequence homogeneity, respectively.S. hydroscopicus(KCTC 9782) was confirmed (Fig. 6c). Finally, non-standard strainsS. albus2 weeks (KCTC 1136, KCTC 1533) are all 100% nucleotide sequence homogeneity, and the standard strain in the phylogenetic treeS. albus(KCTC 1082) was confirmed (Fig. 6d).

In conclusion, in addition to the interspecies variation of each species, the sequence conservation in the same species should be confirmed. Sequence conservation in the same species is demonstrated by nucleotide sequences of 8 non-standard strains. In comparison with each of the 8 strains, the non-standard strains showed 99.7-100% nucleotide sequence homology, and the non-standard strains were identified by 100%.

The present invention will be described in more detail with reference to the following examples, but the following examples are merely illustrative of the present invention, and the scope of the present invention is not limited to the examples.

EXAMPLE

Strain

Micro monospora, Streptomyces 161 strain, in storage at the Korea Biotechnology Research Institute Gene Bank (KCTC), an internationally recognized domestic strain storage center 1 strain,Targeting 162 standard strainsrpoBAnalyze the sequence. In addition, as a non-standard strain to be used for strain identification using the established database, a comparative base sequence analysis method is performed on a total of three strains of eight strains (Table 1).

Example 1: Streptomyces strain specific rpoB Primer manufacturer

Four strains were prepared by aligning the nucleotide sequence in Genbank, determining the nucleotide sequence for creative selection of the characteristic primer site, and requesting it from Genotech. To prepare a primer capable of amplifying the rpoB gene of all Streptomyces strains, S. coelicolor (Genbank No. AL160431), Mycobacterium smeg, which analyzed the entire rpoB sequence in Genbank Matisse ( M. smegmatis (Genbank No. U24494)), Mycobacterium tuberculosis ( M. tuberculosis (Genbank No. L27989)), Mycobacterium leprae ( M. leprae (Genbank No. Z14314)) Compare the 4 week rpoB sequences with each other. The resulting forward primer sites, although belonging to different genera, show a 100% homology with 4 weeks of sequence, Streptomyces. A total of 20 nucleotide sequences from the second base of the amino acid 266 codon to the third base of the codon amino acid 273 based on the copolymeric RNA polymerase subunit B of Coelicol (5'-TC GAC CAC TTC GGC AAC CGC-3 ' ) Can be selected and named SRPOF1 (FIG. 2). The reverse primer site is mycobacteria belonging to slow growing mycobacteria though. Tuberculosis and mycobacteria, a mitobacterium belonging to the rapidly growing mycobacteria, although they differ by one base from leprae. 20 nucleotide sequences showing 100% homology with Segmatis can be selected (FIG. 2) from 2nd base of amino acid 383 codon to 1st base codon of amino acid 377 in reverse direction. SRPOR1 (5'- TC GAT CGG GCA CAT GCG GCC-3 ').

Example 2: Preparation of Streptomyces strain rpoB 306-bp segment

1) DNA extraction

DNA is extracted using the bead beater phenol (BB / P) method. Colonies were harvested, suspended in TEN buffer (Tris-HCl 10 mM, EDTA 1 mM, NaCl 100 mM: pH 8.0), and then 0.1 mm diameter microspheres (0.1 mm diameter; Biospec Products, Bartlesville, Okla., USA) 100 µl (packing volume) and 100 µl of a phenol: chloroform: isopropyl alcohol (50: 49: 1) solution were suspended together and shaken with a mini beater for 1 minute to disrupt the cells. The bacterial lysate was centrifuged at 12000 rpm for 5 minutes and the supernatant (100 μl) was transferred to a new tube. Thereafter, 60 µl of isopropyl alcohol was mixed and again centrifuged at 15000 rpm for 15 minutes. The precipitate was washed with 70% ethanol and then the DNA was recovered in 60 μl of TE buffer (pH 8.0, 10 mM Tris-HCl, 1 mM EDTA).

2) by polymerase chain reaction rpoB Gene amplification

The PCR reaction was performed using AccuPower PCR PreMix (Bionia, Korea) containing 2 U Taq polymerase, 10 mM dNTP, 10 mM Tris-HCl (pH 8.3), 1.5 mM MgCl 2. The primer (Gennotek) prepared in Preparation Example 1 was used. 50 ng of the template DNA of each Streptomyces spp. And 20 pmol of the primers SRPOF1 and SRPOR1 prepared in Preparation Example 1 were added, and distilled water was added so that the final volume was 20 μl. The PCR reaction was carried out with 5 minutes and 30 cycles of the first denaturation at 95 ° C, denaturation at 95 ° C for 1 minute, annealing at 62 ° C for 45 seconds, extended to 72 ° C for 1 minute and 30 seconds, and finally extended to 72 ° C for 5 minutes (Model 9600 thermocycler, Perkin-Elmer cetus). After the polymerase chain reaction, electrophoresis on 1% agarose gel confirmed the reaction product of 352-bp.

As a result of performing polymerase chain reaction using the primer prepared in Preparation Example 1, it was confirmed that 1% agarose gel electrophoresis produced 352-bp rpoB gene segments in all standard strains of 162 strains. 2) As well as Streptomyces genus, it was confirmed that the amplification of the genus Micro monospora belonging to the rare Streptomyces genus strain (lane 3 of Figure 3).

3) Purification of polymerase chain reaction product

After 1% gel electrophoresis, the gel of the reaction product site of Streptomyces strain standard strain 352-bp was cut and then transferred to a new tube to extract DNA. DNA extraction and purification was performed using the Qiaex (Qiagen, Germany) system. 500 μl of the gel dissolution solution QX1 was added to the tube containing the gel, followed by standing at 50 ° C. for 15 minutes to completely dissolve the gel. Thereafter, 10 μl of the gel beads were added to mix, and then left at 50 ° C. for 15 minutes. In the meantime, the tubes are vortexed for 10 seconds at intervals of 1 minute to distribute the beads evenly. Then, washed once with QX1 and twice with QF, dried at 45 ° C. for 10 minutes, and DNA was recovered with 20 μl of TE buffer.

Example 3: rpoB Sequencing of Segments

Automated sequencing uses the gel elution product as template DNA. 60 ng of template DNA, 1.2 pmol of primer, 2 μl of BigDye Terminator Cycle Sequencing kit (PE Appied Biosystems) are mixed, and distilled water is added to prepare a total volume of 10 μl. The reaction was carried out using Perkin Elmer Cetus 9600 at 25 cycles of 95 ° C. 10 seconds, 60 ° C. 10 seconds, and 60 ° C. 4 minutes. DNA was purified from the sample after the reaction by ethanol precipitation. That is, 180 μl of distilled water and 10 μl of 3 M sodium acetate were added to make a total of 200 μl. Then, the mixture was mixed well by adding two volumes of 100% ethanol, followed by centrifugation at 15000 rpm for 20 minutes to precipitate DNA. . Thereafter, 500 µl of 70% ethanol was added thereto, and the DNA was washed by centrifugation at 15000 rpm for 20 minutes. Thereafter, the DNA was recovered by removing form ion (PE Appied Biosystems).

The purified DNA was reacted at 95 ° C. for 5 minutes to form single-stranded DNA, followed by electrophoresis for 2 hours and 30 minutes using an ABI 3100 system (ABI3100, PE Appied Biosystems). In the sequencing method, the nucleotide sequence was analyzed using both the forward primer SRPOF1 and the reverse primer SRPOR1, and the nucleotide sequence of 306-bp excluding the primer site was determined and a database was prepared.

Example 4: rpoB Sequence and Sequence Homology Analysis and Genealogy of Segment (306bp) Sequences

Streptomyces strain rpoB database was constructed by performing alignment of the rpoB sequences (306 bp) of 102 Streptomyces genus standard strains analyzed by Example 3 using the Megalign program of Dnastar software. Multi-alignment was performed by translating the 306-bp base sequence into 101 amino acids in the Megalign program and then aligning the amino acids using the Clustal Method method in the Megalign program. Thus sorted 101 amino acids are converted back to 306 sequences to build a Streptomyces strain identification database.

Sequence homology for each of the 102 standard strain sequences is analyzed using a sequence distance in the Megalign program.

Phylogenetic relationships between each species are analyzed by completing a phylogenetic tree (Kumar, S., K. Tamura, and N. Masatoshi. 1993. MEGA: molecular evolutionary genetics analysis, version 1.01. Pennsylvania State University, University Park. The tree was built using MEGA software. Neighbor-Joining phylogeny based on the Juke-Cantor distance estimation method and the pair wise deletion method was constructed from the 306-bp sequences of 102 sorted strains. Bootstrap analysis was performed with 100 replicates. As a result of the analysis, the nucleotide sequence homogeneity of the rpoB 306-bp fragment and the gene line number using the same are shown in FIGS.

Example 5 Identification of Non-Standard Strains by Comparative Sequence Analysis Method Using rpoB Segment Database of Standard Strains

In order to confirm whether the Streptomyces strain standard strain database can be applied to the identification of bacteria, the non-standard strain Streptomyces olibirobogens Week 1 (KCTC9090), Streptomyces fuseotius 2 weeks (KCTC 9038, KCTC 9242), Streptomyces hydrocopicus 3 weeks (KCTC 9030, KCTC 9031, KCTC 9069),And Streptomyces albus 2 weeks (KCTC 1136, KCTC 1533) A total of four strains were identified by comparative sequencing method.

First, DNA of each strain was extracted, rpoB gene amplified, and purified in the same manner as in Example 2. Then, according to the method of Example 3, each of the 306-bp sequence was analyzed using the purified product as a template.

Each analyzed 306-bp sequence was inserted into the Megalign program of Dnastar software, where 162 sequences were accumulated, and the polymorphism was performed as described above.The phylogenetic tree was then determined by Neighbor-Joining method of Mega software. Completion was performed to identify bacteria. As a result, non-standard strain Streptomyces olibichromogens (KCTC9090) One strain has 100% sequence homology and is a standard strain of Streptomyces olibichromogens on line tree. It could be confirmed that (KCTC9064) is located (Fig. 6a). Nonstandard Strains Streptomyces Fusethius Two weeks (KCTC 9038, KCTC 9242) was shown to be located in the standard strain Streptomyces Fusethius (KCTC 9199) on the phylogenetic tree showing 100%, 99.7% sequence homogeneity, respectively (Fig. 6b). Non-standard strain Streptomyces hydroscopicus 3 weeks (KCTC 9030, KCTC 9031, KCTC 9069) was shown to be located in the standard strain Streptomyces hydroscopicus (KCTC 9782) on the phylogeny while showing nucleotide sequence homogeneity of 100%, 99.7%, and 99.7%, respectively (Fig. 6c). Finally, non-standard strain Streptomyces albus 2 weeks (KCTC 1136, KCTC 1533) was confirmed to be located in the standard strain Streptomyces albus (KCTC 1082) on the phylogenetic tree while all showing 100% nucleotide sequence homogeneity (FIG. 6D).

The present invention relates to a polynucleotide representing a 306-bp segment of an RNA polymerase gene ( rpoB ) that can be used for the identification of Streptomyces strain specific PCR primers and Streptomyces strains, and Streptomyces strains using the same. By providing a method, there is an advantage of providing a simple, economical and accurate identification method by solving the problem of slow growth rate and the presence of various species, and the problems of substance-based identification and 16s rDNA identification.

<110> BIOMEDLAB (represetative: KIM, JONG-WON) KIM, BUM-JUN KUK, YUN-HO <120> primer set specific to genus streptomyces, rpoB gene sequences, and identification method of genus streptomyces by using the same <130> DPP20021682 <160> 164 <170> KopatentIn 1.71 <210> 1 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> forward primer <400> 1 tcgaccactt cggcaaccgc 20 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> reverse primer <400> 2 tcgatcgggc acatgcggcc 20 <210> 3 <211> 306 <212> DNA <213> K.azatica <400> 3 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac cggcctggcc cggatggagc 60 gcgtcgtccg cgagcggatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgtcgt ctgtccgcgc 240 tgggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 4 <211> 306 <212> DNA <213> K. cystarginea <400> 4 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgccc 240 tcggtcccgg tggtctctcc cgtgagcgcg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 5 <211> 306 <212> DNA (213) K. griseola <400> 5 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac cggcctggcc cgcatggagc 60 gcgtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgcgc 240 tgggccccgg cggtctctcc cgtgagcggg cgggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 6 <211> 306 <212> DNA <213> K. mediocidica <400> 6 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggtaccagc cagctgtccc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgtcgt ctgtccgcgc 240 tgggcccggg tggtctgtcc cgtgagcgcg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 7 <211> 306 <212> DNA K. phosalacinea <400> 7 tgcgcaacgt cggcgagctg atccagaacc aggtccgcac cggcctcgcc cgcatggagc 60 gagtggtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgtcgt ctgtccgcgc 240 tcggccccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccct 300 cgcact 306 <210> 8 <211> 306 <212> DNA <213> K. setae <400> 8 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac cggcctggcc cggatggagc 60 gcgtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgcgc 240 tcggccccgg tggtctgtcc cgcgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 9 <211> 306 <212> DNA <213> M. echinospora <400> 9 tgcgtaccgt cggcgagctg atccagaacc aggtccgggt cggcctctcc cgcatggagc 60 gggtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg cccggtggtg gcggcgatca aggagttctt cggcacgtcg cagctgtccc 180 agttcatgga ccagaccaac ccgctggcgg gcctgaccca ccggcgccgg ctgagcgcgc 240 tcggcccggg tggtctgtcc cgggagcggg ccggcttcga ggtccgggac gtgcacccgt 300 cgcact 306 <210> 10 <211> 306 <212> DNA <213> S. abikoensis <400> 10 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtcggcgc 240 tgggtccggg cggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cgcact 306 <210> 11 <211> 306 <212> DNA <213> S. achromogenes <400> 11 tgcgcaacgt cggcgagctg atccagaacc aggtccctac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggtccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 12 <211> 306 <212> DNA S. acrimycini <400> 12 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 13 <211> 306 <212> DNA <213> S. actuosus <400> 13 tccgcaacgt cggcgagctg atccagaacc aggtccgcac gggtctcgcc cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg ggctgacgca caagcgtcgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccgt 300 cgcact 306 <210> 14 <211> 306 <212> DNA <213> S. aculeolatus <400> 14 tccgcaacgt cggcgagctg atccagaacc aggtgcgtac gggcctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtccg gtctgaccca caagcgccgg ctcaacgcgc 240 tcggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 15 <211> 306 <212> DNA S. alanosinicus <400> 15 tccgcaacgt cggcgagctg atccagaacc aggtgcgtac gggtctcgcc cgtatggagc 60 gcgtcgtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 16 <211> 306 <212> DNA <213> S. albireticuli <400> 16 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagacgaac ccgctctcgg gtctgacgca caagcgtcgt ctgtccgcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgagac gtccacccgt 300 cgcact 306 <210> 17 <211> 306 <212> DNA <213> S. albofaciens <400> 17 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gcctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 18 <211> 306 <212> DNA <213> S. alboflavus <400> 18 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccga ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggttcgtgac gtgcacccgt 300 cgcact 306 <210> 19 <211> 306 <212> DNA <213> S. albogriseolus <400> 19 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 20 <211> 306 <212> DNA <213> S. albolongus <400> 20 tgcgcagcgt gggcgagctc atccagaacc aggtccgcac cggcctggcc cgtatggagc 60 gcgtcgtccg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg ggctgacgca caagcggcgt ttgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 21 <211> 306 <212> DNA <213> S. alboniger <400> 21 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggcgc 240 tcggaccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 22 <211> 306 <212> DNA <213> S. albosporeus <400> 22 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg cccggtcgtg gcgtcgatca aggagttctt cggcacctcg cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctctcggcgc 240 tgggtcccgg cggtctgtcc cgtgagcggg ccggctttga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 23 <211> 306 <212> DNA <213> S. alboviridis <400> 23 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgaccca caagcgccgc ctgtccgctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 24 <211> 306 <212> DNA <213> S. albulus <400> 24 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggtctgga cgtccgtgac gtgcacccgt 300 cgcact 306 <210> 25 <211> 306 <212> DNA <213> S. albus <400> 25 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gggtcgtccg cgagcggatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgatctcgg gtctgaccca caagcgccgt ctcaacgcgc 240 tgggcccggg tggtctgagc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 26 <211> 306 <212> DNA S. almquistii <400> 26 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gggtcgtccg cgagcggatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgatctcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgagc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 27 <211> 306 <212> DNA <213> S. aminophilus <400> 27 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgatctcgg gtctcaccca caagcggcgt ctgaactcgc 240 tgggcccggg tggtctgagc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 28 <211> 306 <212> DNA <213> S. antimycoticus <400> 28 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 29 <211> 306 <212> DNA <213> S. argenteolus <400> 29 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg tgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgc ctgtcggcgc 240 tgggcccggg tggcctctcc cgtgagcggg ccggcctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 30 <211> 306 <212> DNA <213> S. armeniacus <400> 30 tgcggaacgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cggatggagc 60 gcgtcgtccg cgagcggatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg cccggtcgtc gcctccatca aggaattctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg ggctcaccca caagcgccgc ctcaacgcgc 240 tgggcccggg cggtctgagc cgtgagcggg cgggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 31 <211> 306 <212> DNA S. avidinii <400> 31 tccgcagcgt cgccgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 32 <211> 306 <212> DNA <213> S. bacillaris <400> 32 tccgcaacgt cggcgagctc atccagaccc aggtccgtac gggtctggcc cggatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gtctcaccca caagcgccgt ctctcggcgc 240 tgggcccggg tggtctctcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 33 <211> 306 <212> DNA <213> S. bambergiensis <400> 33 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttgga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 34 <211> 306 <212> DNA <213> S. bikiniensis <400> 34 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgc ctctcggcgc 240 tcggtcccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cccact 306 <210> 35 <211> 306 <212> DNA S. cacaoi asoensis <400> 35 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgactcagg acgtcgaggc catcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gcctgaccca caagcgccgc ctgtcggcgc 240 tgggccccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 36 <211> 306 <212> DNA <213> S. capillispiralis <400> 36 tccgcaccgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgacgca caagcgtcgt ctcaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 37 <211> 306 <212> DNA <213> S. carpinensis <400> 37 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtccg cgagcggatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctctccg ggctgacgca caagcggcgt ctgtccgcgc 240 tggggccggg tggtccctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 38 <211> 306 <212> DNA S. catenulae <400> 38 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg tgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gtctgaccca caagcgccgt ctgtccgcgc 240 tgggccccgg cggtctctcc cgtgagcggg ccggtctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 39 <211> 306 <212> DNA <213> S. celluloflavus <400> 39 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gtctcaccca caagcgccgt ctctcggcgc 240 tcggcccggg tggtctctcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 40 <211> 306 <212> DNA <213> S. chartreusis <400> 40 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg tgagcgcatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 41 <211> 307 <212> DNA <213> S. chattanoogensis <400> 41 tgcgcacccg tcggcgagct catccagaac caggtccgta cgggtctggc ccgtatggag 60 cgcgtcgtgc gtgagcgcat gaccacccag gacgtcgagg cgatcacgcc gcagaccctg 120 atcaacatcc ggccggtcgt cgcctccatc aaggagttct tcggcaccag ccagctgtcg 180 cagttcatgg accagaccaa cccgctgtcg ggtctgaccc acaagcgccg tctgtcggcg 240 ctgggcccgg gtggtctctc ccgtgagcgg gccggcctgg acgtccgtga cgtgcacccc 300 tcgcact 307 <210> 42 <211> 306 <212> DNA <213> S. chrysomallus <400> 42 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctcgcc cgcatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgccc 240 tcggtcccgg tggtctctcc cgtgagcgcg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 43 <211> 306 <212> DNA <213> S. cinereoruber <400> 43 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgactcagg acgtcgaggc catcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctctcggcgc 240 tcggtccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 44 <211> 306 <212> DNA S. cinereus <400> 44 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtccacccgt 300 cgcact 306 <210> 45 <211> 306 <212> DNA <213> S. cinnamonensis <400> 45 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gcctgaccca caagcgccgc ctgtcggcgc 240 tgggccccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 46 <211> 306 <212> DNA <213> S. cirratus <400> 46 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccgt 300 cgcact 306 <210> 47 <211> 306 <212> DNA <213> S. clavuligerus <400> 47 tgcgcaacgt cagcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gtgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccc cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 tcggcccggg tggtctctcc cgtgagcggg cgggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 48 <211> 306 <212> DNA <213> S. coelicolor <400> 48 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 49 <211> 306 <212> DNA <213> S. coeruleorubidus <400> 49 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtgcg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg gtctgacgca caagcggcgt ctgtccgcgc 240 tcggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 50 <211> 306 <212> DNA S. collinus <400> 50 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 51 <211> 306 <212> DNA <213> S. corchorusii <400> 51 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagt cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 52 <211> 306 <212> DNA <213> S. crystallinus <400> 52 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gggtcgtccg cgagcggatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 53 <211> 306 <212> DNA <213> S. cuspidosporus <400> 53 tccgcaacgt cggcgagctg atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtccg cgagcgcatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gtctgaccca caagcgccgt ctgaacgcgc 240 tcggccccgg tggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cgcact 306 <210> 54 <211> 306 <212> DNA <213> S. cyaneus <400> 54 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac cggtctggct cgtatggagc 60 gcgtcgtccg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 55 <211> 306 <212> DNA <213> S. diasticus <400> 55 tgcgtaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggctc 240 ttggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 56 <211> 306 <212> DNA <213> S. djakartensis <400> 56 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcggcgt ctgtccgcgc 240 tcggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 57 <211> 306 <212> DNA <213> S. durhamensis <400> 57 tccgcagcgt cggtgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gtgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg cccggtcgtg gcgtcgatca aggagttctt cggcacctcg cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 58 <211> 306 <212> DNA <213> S. echinoruber <400> 58 tgcgcagcgt cggcgagctg atccagaacc aggtgcgcac cggtctcgcc cgtatggagc 60 gggtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctcaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 59 <211> 306 <212> DNA <213> S. ederensis <400> 59 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg tgagcggatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctcaacgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga agtccgtgac gtgcacccct 300 cgcact 306 <210> 60 <211> 306 <212> DNA <213> S. ehimensis <400> 60 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtcggcgc 240 tgggtccggg cggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtccacccgt 300 cgcact 306 <210> 61 <211> 306 <212> DNA <213> S. flaveolus <400> 61 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gggtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctcaacgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 62 <211> 306 <212> DNA <213> S. flavofuscus <400> 62 tgcgcagcgt cggcgagctg atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gtctgaccca caagcgccgt ctgaacgcgc 240 tcggtcccgg tggtctgtcc cgcgagcggg cgggtttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 63 <211> 306 <212> DNA <213> S. fradiae <400> 63 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cggatggagc 60 gcgtcgtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtccg ggctgacgca caagcgtcgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cccact 306 <210> 64 <211> 306 <212> DNA <213> S. galilaeus <400> 64 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gggtcgtgcg tgagcgcatg accactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctcaacgccc 240 tcggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 65 <211> 306 <212> DNA <213> S. globisporus <400> 65 tgcgcaacgt gggcgagctc atccagaacc aggtccgtac gggcctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cccact 306 <210> 66 <211> 306 <212> DNA <213> S. griseochromogenes <400> 66 tgcgcagcgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gtgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 67 <211> 306 <212> DNA <213> S. griseolus <400> 67 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gggtcgtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtccg gtctcaccca caagcgccgt ctctcggcgc 240 tcggcccggg tggtctctcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 68 <211> 306 <212> DNA <213> S. griseoviridis <400> 68 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacattcg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggcgc 240 tcggtccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 69 <211> 306 <212> DNA <213> S. griseus griseus <400> 69 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgctc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cgcact 306 <210> 70 <211> 306 <212> DNA <213> S. hiroshimensis <400> 70 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgt ctctcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccgt 300 cgcact 306 <210> 71 <211> 306 <212> DNA <213> S. hygroscopicus <400> 71 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 72 <211> 306 <212> DNA <213> S. libani libani <400> 72 tccgcaacgt cggcgagctg atccagaccc aggtccgtac gggtctggct cggatggagc 60 gcgtcgtgcg tgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc aagttgtcca 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtccgcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 73 <211> 306 <212> DNA <213> S. limosus <400> 73 tgcgcaacgt cggcgagctg atccagaacc aggtccgcac gggtctcgcc cgtatggagc 60 gtgtcgtgcg tgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gtctcaccca caagcgccgt ctgtcggcgc 240 tcggcccggg tggtctctcg cgtgagcggg ccggtttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 74 <211> 306 <212> DNA S. lincolnensis <400> 74 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gagtcgtccg tgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctctcgc 180 agttcatgga ccagaacaac ccgctgtccg gtctgacgca caagcgtcgt ctctcggcgc 240 tgggccccgg tggtctgtcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgc 300 cccact 306 <210> 75 <211> 306 <212> DNA <213> S. longwoodensis <400> 75 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cgtatggagc 60 gcgtcgtccg caagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gcccgtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 76 <211> 306 <212> DNA S. melanogenes <400> 76 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 77 <211> 306 <212> DNA <213> S. minutiscleroticus <400> 77 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctctcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgc ctctcggcgc 240 tgggtcccgg cggtctgtcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 78 <211> 306 <212> DNA <213> S. nitrosporeus <400> 78 tgcgtaacgt cggcgagctc atccagaacc aggtccgcac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gtctcaccca caagcgccgt ctctcggcgc 240 tcggcccggg tggtctctcg cgtgagcggg ccggcttcga ggtccgcgac gttcacccgt 300 cccact 306 <210> 79 <211> 306 <212> DNA <213> S. noboritoensis <400> 79 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtgcg gtctcaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 80 <211> 306 <212> DNA <213> S. nodosus <400> 80 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg gcctgacgca caagcggcgt ctgtccgcgc 240 tgggcccggg cggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 81 <211> 306 <212> DNA <213> S. nojiriensis <400> 81 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgacccagg acgtcgaggc catcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gcctgaccca caagcgccgc ctgtcggcgc 240 tgggccccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccgt 300 cgcact 306 <210> 82 <211> 306 <212> DNA <213> S. olivaceoviridis <400> 82 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gagtcgtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagactctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 83 <211> 306 <212> DNA <213> S. olivochromogenes <400> 83 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gcccgtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 84 <211> 306 <212> DNA <213> S. pactum <400> 84 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac cggtctggcc cgtatggagc 60 gcgtcgtccg cgagcggatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgctgtcgg gtctggccca caagcgccgc ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 85 <211> 306 <212> DNA <213> S. paradoxus <400> 85 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 86 <211> 306 <212> DNA <213> S. peucetius <400> 86 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggtccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 87 <211> 306 <212> DNA <213> S. phaeochromogenes <400> 87 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg tgagcggatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgacgca caagcgtcgt ctcaacgcgc 240 tcggcccggg tggtctgtcc cgtgagcggg ccggcttcga agtccgtgac gtgcacccct 300 cgcact 306 <210> 88 <211> 306 <212> DNA <213> S. plicatus <400> 88 tccgcagcgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctcaacgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccct 300 cgcact 306 <210> 89 <211> 306 <212> DNA <213> S. pulveraceus <400> 89 tccgtaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gtgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg gcctgacgca caagcgccgt ctcaacgcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcttcga agtccgtgac gtgcacccgt 300 cgcact 306 <210> 90 <211> 306 <212> DNA <213> S. rameus <400> 90 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 91 <211> 306 <212> DNA <213> S. rimosus rimosus <400> 91 tccgcaacgt cggcgagctg atccagaacc aggtccgtac ggctctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gcctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 92 <211> 306 <212> DNA <213> S. roseosporus <400> 92 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcggcgt ctgtccgcgc 240 tcggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 93 <211> 306 <212> DNA <213> S. sclerotialus <400> 93 tccgtaacgt cggtgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcccccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgacgca caagcgtcgt ctcaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gttcacccct 300 cgcact 306 <210> 94 <211> 306 <212> DNA <213> S. setonii <400> 94 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg gtctgacgca caagcgtcgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 95 <211> 306 <212> DNA <213> S. sioyaensis <400> 95 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggtctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 96 <211> 306 <212> DNA <213> S. somaliensis <400> 96 tgcgcaccgt cggcgagctc atccagaccc aggtccgcac gggcctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtccg gtctgacgca caagcgccgt ctgtccgcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 97 <211> 306 <212> DNA <213> S. spectabilis <400> 97 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcatcaccca caagcggcgt ctgaactcgc 240 tcggcccggg tggtctctcc cgtgagcggg cgggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 98 <211> 306 <212> DNA <213> S. subrutilus <400> 98 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcatccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctctcggctc 240 tgggacccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 99 <211> 306 <212> DNA <213> S. tubercidicus <400> 99 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 100 <211> 306 <212> DNA <213> S. vinaceus <400> 100 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtccg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctttcggcgc 240 tgggtcccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 101 <211> 306 <212> DNA <213> S. violarus <400> 101 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcggatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 102 <211> 306 <212> DNA <213> S. violascens <400> 102 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggcgc 240 tcggcccggg tggtctctcg cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 103 <211> 306 <212> DNA <213> S. virginiae <400> 103 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctctcgg gcctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg cggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccgt 300 cgcact 306 <210> 104 <211> 306 <212> DNA <213> S. xantophaeus <400> 104 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtccg cgagcgcatg acgactcagg acgtcgaggc catcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctcaccca caagcgccgc ctgtcggctc 240 ttggtcccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 105 <211> 306 <212> DNA <213> S.albaduncus <400> 105 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtccacccgt 300 cgcact 306 <210> 106 <211> 306 <212> DNA <213> S.althioticus <400> 106 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgcca 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 107 <211> 306 <212> DNA <213> S.ambofaciens <400> 107 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gggtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtccacccgt 300 cgcact 306 <210> 108 <211> 306 <212> DNA <213> S.anulatus <400> 108 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgaccca caagcgccgt ctgaacgccc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 109 <211> 306 <212> DNA <213> S.anthocyanicus <400> 109 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 110 <211> 306 <212> DNA <213> S. cellulose <400> 110 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 111 <211> 306 <212> DNA <213> S.armeniacus <400> 111 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 112 <211> 306 <212> DNA <213> S. coelescens <400> 112 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 113 <211> 306 <212> DNA <213> S.griseoflavus <400> 113 tgcgcaacgt cggcgagctg atccagaacc aggtccgcac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgacgca caagcgtcgt ctgaacgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 114 <211> 306 <212> DNA <213> S.humiferus <400> 114 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 115 <211> 306 <212> DNA <213> S.lividans <400> 115 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtccacccgt 300 cgcact 306 <210> 116 <211> 306 <212> DNA <213> S.murinus <400> 116 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg gccacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agtccatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 117 <211> 306 <212> DNA <213> S.pilosus <400> 117 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctgaacgccc 240 ttggcccggg tggtctctcc cgtgagcggg ccggctttga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 118 <211> 306 <212> DNA <213> S.rubiginosus <400> 118 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggcctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 119 <211> 306 <212> DNA <213> S.tendae <400> 119 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 120 <211> 306 <212> DNA <213> S.umbrinus <400> 120 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg tgagcggatg acgactcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gcctgacgca caagcgtcgt ctcaacgcgc 240 tcggcccggg gggtctgtcc cgtgagcggg ccggcttcga agtccgtgac gtgcacccct 300 cgcact 306 <210> 121 <211> 306 <212> DNA <213> S.violaceoruber <400> 121 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cgcact 306 <210> 122 <211> 306 <212> DNA <213> S.xanthocidicus <400> 122 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg tgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 123 <211> 306 <212> DNA <213> S. yokosukanens <400> 123 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg ggctgacgca caagcgtcgt ctgaacgccc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtccacccct 300 cgcact 306 <210> 124 <211> 306 <212> DNA <213> S. amakusaensis <400> 124 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 125 <211> 306 <212> DNA <213> S.aburaviensis <400> 125 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccaagaccac ccgctgtcgg gtctgaccca caagcgccgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcgcg ccggctttga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 126 <211> 306 <212> DNA <213> S. albospinus <400> 126 tgcgtaacgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cggatggagc 60 gcgtcgtgcg tgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgcgac gtgcacccgt 300 cccact 306 <210> 127 <211> 306 <212> DNA <213> S. albovinaceous <400> 127 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cccact 306 <210> 128 <211> 306 <212> DNA <213> S.anabdii <400> 128 tgcgcagcgt gggcgagctc atccagaacc aggtccgcac cggcctggcc cgtatggagc 60 gcgtcgtccg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg ggctgacgca caagcggcgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 129 <211> 306 <212> DNA <213> S.antibioticus <400> 129 tccgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtctg gtctgacgca caagcgccgt ctgtcggcgc 240 tcggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 130 <211> 306 <212> DNA <213> S.atroolvaceous <400> 130 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtccgcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 131 <211> 306 <212> DNA <213> S.aureufaciens <400> 131 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgcatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaccaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgccc 240 tcggtccggg tggtctctcc cgtgagcgcg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 132 <211> 306 <212> DNA <213> S.azureus <400> 132 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg aggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 133 <211> 306 <212> DNA <213> S.baldacii <400> 133 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 134 <211> 306 <212> DNA <213> S. candidus <400> 134 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggtcccgg tggtctgtcc cgtgagcggg ccggctttga ggtccgagac gtgcacccct 300 cgcact 306 <210> 135 <211> 306 <212> DNA <213> S.caseius <400> 135 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcta ggtccgtgac atgcacccgt 300 cccact 306 <210> 136 <211> 306 <212> DNA <213> S.californicus <400> 136 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 137 <211> 306 <212> DNA <213> S. carpinensis <400> 137 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcc cgtatggagc 60 gcgtcgtccg cgagcggatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctctccg ggctgacgca caagcggcgt ctgtccgcgc 240 tggggccggg tggtccctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 138 <211> 306 <212> DNA <213> S.chromogenes <400> 138 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <139> <211> 306 <212> DNA <213> S.cinnamoneus <400> 139 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctgtccgcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cgcact 306 <210> 140 <211> 306 <212> DNA <213> S.citreofluorescens <400> 140 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctcgcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gcctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcctgga cgtccgtgac gtgcacccct 300 cgcact 306 <210> 141 <211> 306 <212> DNA <213> S. coerulescens <400> 141 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgccgt ctgtccgcgc 240 tcggcccggg tggtctgtcc cgtgagcggc ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 142 <211> 306 <212> DNA <213> S. coeruleofuscus <400> 142 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 143 <211> 306 <212> DNA <213> S.coralus <400> 143 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgtcgt ctgtcggcgc 240 tgggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 144 <211> 306 <212> DNA <213> S.cremeus <400> 144 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 145 <211> 306 <212> DNA <213> S.cyaneofuscatus <400> 145 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 146 <211> 306 <212> DNA <213> S. disatochromogenes <400> 146 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gtgtcgtccg cgagcgcatg acgacccagg acgtcgaggc gatcacgccc cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgacgca caagcgtcgt ctctcggcac 240 tgggtcccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 147 <211> 306 <212> DNA <213> S.erumpens <400> 147 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggcctggcg cggatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggctc 240 tcggcccggg tggcctctcc cgtgagcggg ccggcttcga ggtccgagac gtgcacccgt 300 cccact 306 <210> 148 <211> 306 <212> DNA <213> S.erythraeus <400> 148 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac cggtctggcc cgtatggagc 60 gcgtcgtccg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaccaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgagc cgtgagcggg cgggcttcga ggtccgtgac gtgcacccgt 300 cccact 306 <210> 149 <211> 306 <212> DNA <213> S.eurythermus <400> 149 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 150 <211> 306 <212> DNA <213> S.fimbriatus <400> 150 tgcgcagcgt cggcgagctc atccagaacc aggtccgcac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacgcagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtccg ggctgacgca caagcgtcgt ctgtccgcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 151 <211> 306 <212> DNA <213> S.flavotricini <400> 151 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 152 <211> 306 <212> DNA <213> S.flavovirens <400> 152 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 153 <211> 306 <212> DNA <213> S.fulvissimus <400> 153 tgcgcaacgt cggcgagctc atccagaacc aggtccgcac gggtctggct cgtatggagc 60 gcgtcgtgcg tgagcgcatg acgacgcagg acgtcgaggc gatcacgccg cagacgctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctcaccca caagcgccgc ctgtcggctc 240 ttggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 ctcact 306 <210> 154 <211> 306 <212> DNA <213> S.fumanus <400> 154 tgcgcaacgt cggcgagctg atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 155 <211> 306 <212> DNA <213> S.gougeroti <400> 155 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggccccgg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 156 <211> 306 <212> DNA <213> S.griseoruber <400> 156 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 157 <211> 306 <212> DNA <213> S.griseolosporeus <400> 157 tgcgcaacgt cggcgagctg atccagaacc aggtccgcac cggcctcgcc cgcatggagc 60 gggtggtccg cgagcgcatg accacccagg acgtcgaggc gatcacgccg caaaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagacgaac ccgctgtcgg gcctgaccca caagcgccgt ctgtccgcgc 240 tcggcccggg tggtctgtcc cgcgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 158 <211> 306 <212> DNA <213> S.griseostramineus <400> 158 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 159 <211> 306 <212> DNA <213> S. hachijoense <400> 159 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 160 <211> 306 <212> DNA <213> S.halstedii <400> 160 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 161 <211> 306 <212> DNA <213> S.humidus <400> 161 tgcgcaacgt cggcgagctg attcagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtcgc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tcggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 162 <211> 306 <212> DNA <213> S.indigoferus <400> 162 tgcgcaacgt cggcgagctc atccagaacc aggtccgtac gggtctggcc cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctgtcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306 <210> 163 <211> 306 <212> DNA <213> S.kifunensis <400> 163 tgcgcagcgt cggcgagctc atccagaacc aggtccgtac gggtctggcg cgtatggagc 60 gcgtcgtgcg cgagcgcatg accacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggctttga ggtccgtgac gtgcacccct 300 cgcact 306 <210> 164 <211> 306 <212> DNA <213> S.kurssanovi <400> 164 tgcgtaacgt cggcgagctc atccagaacc aggtccgtac gggtctggct cgtatggagc 60 gcgtcgtgcg cgagcgcatg acgacccagg acgtcgaggc gatcacgccg cagaccctga 120 tcaacatccg gccggtcgtc gcctccatca aggagttctt cggcaccagc cagctgtccc 180 agttcatgga ccagaacaac ccgctgtcgg gtctgaccca caagcgccgt ctgtcggcgc 240 tgggcccggg tggtctctcc cgtgagcggg ccggcttcga ggtccgtgac gtgcacccgt 300 cgcact 306

Claims (9)

Amplify specifically the RNA polymerase B subunit gene (rpoB) segment of Streptomyces species, a primer comprising the nucleotide sequence represented by SEQ ID NO: 1, and a primer comprising the nucleotide sequence represented by SEQ ID NO: 2 At least one primer selected from the group consisting of. Selected from the group consisting of the nucleotide sequences shown in SEQ ID NO: 3 to SEQ ID NO: 164, a total of 306- ranging from the second base of the 274th amino acid codon of the rpoB gene of the Streptomyces genus to the first base of the 376 amino acid codon Polynucleotide comprising a bp sequence. (1) amplifying the rpoB gene segment of the target strain using primers that specifically amplify the rpoB gene segment of the Streptomyces strain, (2) analyzing the nucleotide sequence of the amplified rpoB gene segment, (3) A method of detecting or identifying a Streptomyces sp. Strain using the rpoB gene segment, comprising comparing the nucleotide sequence obtained in step (2) with the nucleotide sequence of the rpoB gene segment of the standard strain. The method of claim 3, wherein the primer has a nucleotide sequence shown in SEQ ID NO: 1 to SEQ ID NO: 2. The method according to claim 3 or 4, wherein the rpoB gene segment of the standard strain is selected from the group consisting of the nucleotide sequences shown in SEQ ID NO: 3 to SEQ ID NO: 164. The method according to claim 3, wherein in step (3), the homology between the nucleotide sequence of the rpoB gene segment of the target strain and the rpoB gene segment of the standard strain is determined based on 99.7% or more. 4. The method according to claim 3, wherein in step (3), the nucleotide sequence of the rpoB gene segment of the target strain is substituted into the nucleotide sequence of the rpoB gene segment of the standard strain to complete and determine the phylogenetic tree after multialignment. The method according to claim 5, wherein in step (3), a homology between the nucleotide sequence of the rpoB gene segment of the target strain and the rpoB gene segment of the standard strain is selected to be 99.7% or more. The method according to claim 5, wherein in step (3), in step (3), the nucleotide sequence of the rpoB gene segment of the target strain is multi-arranged to the nucleotide sequence of the rpoB gene segment of the standard strain, and then the complete flow chart is determined. How to feature.