KR100461776B1 - A preparing Method for rice containing beta-glucan - Google Patents
A preparing Method for rice containing beta-glucan Download PDFInfo
- Publication number
- KR100461776B1 KR100461776B1 KR10-2001-0071378A KR20010071378A KR100461776B1 KR 100461776 B1 KR100461776 B1 KR 100461776B1 KR 20010071378 A KR20010071378 A KR 20010071378A KR 100461776 B1 KR100461776 B1 KR 100461776B1
- Authority
- KR
- South Korea
- Prior art keywords
- rice
- glucan
- beta
- beta glucan
- solution
- Prior art date
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- 229920002498 Beta-glucan Polymers 0.000 title claims abstract description 89
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 title claims abstract description 86
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 65
- 235000009566 rice Nutrition 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims description 21
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 64
- 239000000243 solution Substances 0.000 claims abstract description 33
- 239000012670 alkaline solution Substances 0.000 claims abstract description 10
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 10
- 239000011707 mineral Substances 0.000 claims abstract description 10
- 238000005507 spraying Methods 0.000 claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 26
- 235000019798 tripotassium phosphate Nutrition 0.000 claims description 13
- 229910000404 tripotassium phosphate Inorganic materials 0.000 claims description 13
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000011248 coating agent Substances 0.000 claims description 7
- 238000000576 coating method Methods 0.000 claims description 7
- 238000007598 dipping method Methods 0.000 claims description 7
- 229960004203 carnitine Drugs 0.000 claims description 6
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 5
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 5
- 239000001488 sodium phosphate Substances 0.000 claims description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims description 5
- 235000019801 trisodium phosphate Nutrition 0.000 claims description 5
- 229910000406 trisodium phosphate Inorganic materials 0.000 claims description 5
- 230000001965 increasing effect Effects 0.000 claims description 4
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 4
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 4
- PHIQHXFUZVPYII-ZCFIWIBFSA-O (R)-carnitinium Chemical compound C[N+](C)(C)C[C@H](O)CC(O)=O PHIQHXFUZVPYII-ZCFIWIBFSA-O 0.000 claims 1
- 235000013339 cereals Nutrition 0.000 abstract description 8
- 230000000975 bioactive effect Effects 0.000 abstract description 6
- 239000000126 substance Substances 0.000 abstract description 5
- 230000002708 enhancing effect Effects 0.000 abstract description 4
- 230000036039 immunity Effects 0.000 abstract description 2
- 238000007602 hot air drying Methods 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 14
- 238000001035 drying Methods 0.000 description 9
- 229920002558 Curdlan Polymers 0.000 description 7
- 239000001879 Curdlan Substances 0.000 description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 7
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 7
- 229940078035 curdlan Drugs 0.000 description 7
- 235000019316 curdlan Nutrition 0.000 description 7
- 235000010755 mineral Nutrition 0.000 description 7
- 239000011591 potassium Substances 0.000 description 7
- 229910052700 potassium Inorganic materials 0.000 description 7
- 229920001503 Glucan Polymers 0.000 description 6
- PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 241000589158 Agrobacterium Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000523 sample Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010066477 Carnitine O-acetyltransferase Proteins 0.000 description 1
- 102100036357 Carnitine O-acetyltransferase Human genes 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical class Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 1
- 230000005965 immune activity Effects 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000009616 inductively coupled plasma Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229930185723 monacolin Natural products 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 229940032330 sulfuric acid Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/196—Products in which the original granular shape is maintained, e.g. parboiled rice
- A23L7/1963—Products in which the original granular shape is maintained, e.g. parboiled rice coated with a layer
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/101—Addition of antibiotics, vitamins, amino-acids, or minerals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P20/00—Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
- A23P20/10—Coating with edible coatings, e.g. with oils or fats
- A23P20/15—Apparatus or processes for coating with liquid or semi-liquid products
- A23P20/17—Apparatus or processes for coating with liquid or semi-liquid products by dipping in a bath
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P20/00—Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
- A23P20/10—Coating with edible coatings, e.g. with oils or fats
- A23P20/15—Apparatus or processes for coating with liquid or semi-liquid products
- A23P20/18—Apparatus or processes for coating with liquid or semi-liquid products by spray-coating, fluidised-bed coating or coating by casting
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/02—Acid
- A23V2250/06—Amino acid
- A23V2250/0612—Carnitine
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/502—Gums
- A23V2250/5034—Beta-Glucan
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Botany (AREA)
- Mycology (AREA)
- Cereal-Derived Products (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
본 발명은 면역증강능이 우수하고 필름 형성능이 있는 베타글루칸으로 코팅된 기능성 쌀 및 그 제조방법에 관한 것이다. 베타글루칸을 미네랄 및 생리활성 물질을 포함한 알칼리 용액에 용해시킨 후, 일정 양의 쌀을 베타글루칸 용액에 침지하거나 용액을 쌀에 분무하여 자연 상태 또는 열풍 건조하여 쌀을 코팅한다. 코팅된 곡물은 베타글루칸 그리고 미네랄 등을 포함하여 인체의 면역력을 증가시키며 건강에 효과적인 기능을 갖게 된다.The present invention relates to a functional rice coated with beta glucan having excellent immune enhancing ability and film forming ability, and a method of manufacturing the same. After dissolving beta glucan in an alkaline solution containing minerals and bioactive substances, the rice is coated by immersing a certain amount of rice in the beta glucan solution or spraying the solution into the rice by natural or hot air drying. Coated grains, including beta glucan and minerals, increase the body's immunity and have an effective function for health.
Description
최근 쌀을 비롯한 곡물의 기능성을 향상시키기 위한 많은 연구가 진행되고 있다. 모나콜린을 함유한 홍국미 또는 상황버섯을 쌀에 배양한 상황미(대한민국 특허 공개번호2000-0036716) 등이 많은 관심을 끌고 있다. 키토산으로 코팅한 쌀은 수분건조를 막고 영양손실을 줄일 수 있는 것으로 보고되었다(대한민국특허 공개번호 2001-0044203).Recently, many studies have been conducted to improve the functionality of grains including rice. Honggumi or Monacolin containing Monacholine or Shangmi (cultivated in Korean Patent Publication No. 2000-0036716) in rice has attracted much attention. Rice coated with chitosan has been reported to prevent moisture drying and reduce nutrient loss (Korean Patent Publication No. 2001-0044203).
베타글루칸은 여러 식용버섯, 효모, 보리, 귀리 등에 함유되어 있으며 애그로박테리움 속 미생물에 의해서도 생산된다. 분자 구조로 볼 때 분자량, 가지의 유무 등에 따라 상이한 종류의 베타글루칸들이 다수 있으나 공통적으로는 주 골격으로 포도당이 베타 1,3 결합을 한 것이 특징이다. 이러한 베타글루칸의 일반적인 특징은 동물 및 인체의 면역기능을 향상시키는 것으로 널리 알려져 있다. 일반적으로 보리나 귀리 등의 잡곡류가 영양학적을 쌀보다 우수하다고 알려져 있는 것도 쌀에는 베타글루칸이 없으나 잡곡류는 소량의 베타글루칸을 함유하고 있기 때문이다.Beta-glucans are found in many edible mushrooms, yeast, barley and oats, and are also produced by microorganisms in the genus Agrobacterium. In terms of molecular structure, there are many different types of beta glucans depending on molecular weight and the presence or absence of eggplants. A general feature of these betaglucans is widely known to enhance immune function in animals and the human body. In general, grains such as barley and oats are known to be nutritionally superior to rice because rice has no beta glucan, but cereals contain a small amount of beta glucan.
이에 본 발명자들은 쌀의 기능성을 향상시키기 위하여 쌀 표면에 베타글루칸을 코팅함으로써 베타글루칸을 함유한 쌀을 제조하게 되었다.Accordingly, the present inventors have prepared a beta-glucan-containing rice by coating the beta-glucan on the rice surface in order to improve the functionality of the rice.
일본 특개평 제03-83546호는 불용성 커들란을 물에 현탁시킨 것을 이용하여 조리된 밥의 표면에 커들란을 부착시킨 후 85℃ 이상으로 열을 가하여 커들란이 코팅 필름을 형성하도록 한 코팅된 음식의 제조방법에 대해 개시하고 있다.Japanese Patent Application Laid-Open No. 03-83546 uses a suspension of insoluble curdlan in water to attach the curdlan to the surface of cooked rice, and then heats it to 85 ° C. or higher to coat the curdlan to form a coating film. Disclosed is a method for preparing food.
또한, 일본 특개평 제03-123454호는 곡물의 외관을 향상시키고 저장성을 높이기 위하여 커들란 현탁액을 50∼70℃로 가열하여 건조 곡물의 표면에 커들란 코팅필름을 형성시키는 방법에 대해 개시하고 있다.In addition, Japanese Patent Laid-Open No. 03-123454 discloses a method for forming a curdlan coating film on the surface of dried grains by heating the curdula suspension at 50 to 70 ° C. in order to improve the appearance of the grain and increase the shelf life. .
그러나, 상기 일본공개특허에 개시된 기술들은 글루칸의 일종인 커들란이 불용성임에도 불구하고 조리된 식품이나 건조곡물에 물에 현탁시킨 형태의 커들란을 적용시키고 있어 실제로 커들란이 식품에 균일한 농도로 코팅되기가 어려우며, 또한 가열공정이 필요하여 가열로 인한 타 영양소가 파괴되거나 맛이 저감될 우려가 있다.However, the techniques disclosed in the Japanese Laid-open Patent disclose that even though the curdlan, which is a kind of glucan, is applied to the cooked food or dried grains in a form suspended in water, the curdlan is uniformly dispersed in the food. It is difficult to coat, and there is a fear that the heating process is required and other nutrients due to heating are destroyed or the taste is reduced.
따라서, 본 발명의 목적은 면역증강능이 우수하고 필름 형성능이 있는 베타글루칸으로 쌀을 코팅하여 쌀의 파손을 억제하고, 쌀에는 없던 베타글루칸 그리고 미네랄 등을 부여함으로써, 인체의 면역력을 증가시키며 건강 증진에 효과적인 기능성 곡물을 제조하는 방법을 제공하려는 것이다.Accordingly, an object of the present invention is to coat rice with beta glucan with excellent immune enhancing ability and film forming ability to inhibit breakage of rice, and to impart beta glucan and minerals, which are not present in rice, to increase the immunity of the human body and promote health. It is intended to provide a method for producing functional grains that are effective.
또한, 본 발명의 또다른 목적은 가열공정 없이 간단한 방법으로 균일한 농도의 베타글루칸을 쌀에 코팅하려는 것이다.Still another object of the present invention is to coat betaglucan of uniform concentration on rice in a simple manner without heating.
본 발명에서 사용한 베타글루칸은 알칼리제네스 속 또는 애그로박테리움 속 균주의 발효로 생산하는 다당(polysaccharide)으로서 열을 가하면 응고되는 성질이 있기 때문에 일명 커들란(curdlan)으로도 불리운다. 이 베타글루칸은 중성의 pH에서 물에 녹지 않으며 알칼리 용액에 잘 녹는다. 또한 이 베타글루칸은 필름 형성능이 매우 우수하여 본 발명의 재료로 사용하였다.Beta glucan used in the present invention is a polysaccharide produced by the fermentation of the genus Algene genesis or Agrobacterium, so it is also called a curdlan because it has a property of coagulation when heat is applied. This betaglucan is insoluble in water at neutral pH and soluble in alkaline solution. In addition, this beta glucan was used as the material of the present invention because of its excellent film forming ability.
베타글루칸을 용해하기 위하여 사용한 알칼리제는 인체에 해를 미치지 않는 것이면 무엇이든 가능하며 특별히 제한되지는 않으나, 좀더 바람직하게는 수산화나트륨, 수산화칼륨, 암모니아수, 인산3칼륨, 인산3나트륨 및 이러한 알칼리제의 혼합물을 사용하였다.Alkaline used to dissolve beta glucan can be any one that does not harm the human body, and is not particularly limited, but more preferably sodium hydroxide, potassium hydroxide, aqueous ammonia, tripotassium phosphate, trisodium phosphate, and mixtures of these alkaline agents Was used.
상기 알칼리 성분을 0.01 ~ 5중량%가 되도록 용액을 만든 후 0.1 ~ 5.0중량 %의 베타글루칸을 용해시킨다. 알칼리 용액 중의 알칼리 성분 농도는 0.01중량% 미만이면 베타 글루칸이 거의 용해되지 않으며, 5중량%를 초과하는 경우에는 pH 및 미각에서 바람직하지 않다. 베타글루칸 함량은 0.1∼5.0중량%가 바람직한데, 0.1중량% 미만인 경우에는 면역활성증가 등의 효과가 미미하고, 5.0중량%를 넘는 경우에는 균질한 코팅이 형성되기 어려우며, 용해가 어려워진다.After the solution is prepared so that the alkali component is 0.01 to 5% by weight, 0.1 to 5.0% by weight of beta glucan is dissolved. If the alkali component concentration in the alkaline solution is less than 0.01% by weight, the beta glucan hardly dissolves, and if it exceeds 5% by weight, it is not preferable at pH and taste. The content of beta glucan is preferably 0.1 to 5.0% by weight, but less than 0.1% by weight, the effect of increasing immune activity is insignificant, and when it is more than 5.0% by weight, it is difficult to form a homogeneous coating and it becomes difficult to dissolve.
베타글루칸 용액을 제조할 때 필요에 따라 기능성을 갖는 미네랄이나 생리활성 물질을 첨가할 수 있다. 베타글루칸 자체에 점성이 있어 미네랄 또는 생리활성물질을 용이하게 쌀에 부착시킬 수 있는 것이다. 미네랄 또는 생리활성물질로는 pH의 변화에 따라 구조 및 기능상 크게 영향받지 않는 물질이면 특별한 제한은 없다.When preparing a beta glucan solution, minerals or bioactive substances having functionalities may be added as necessary. The beta glucan itself is viscous so that minerals or bioactive substances can be easily attached to rice. The mineral or bioactive material is not particularly limited as long as the material is not significantly affected in structure and function according to the change in pH.
청결한 쌀을 베타글루칸 용액에 담그거나 쌀에 베타글루칸 용액을 분무함으로써 쌀 표면에 베타글루칸을 도포한다. 도포 후 상온 또는 열풍 건조기에서 쌀을 건조함으로써 베타글루칸을 함유한 쌀을 생산하게 된다.Betaglucan is applied to the rice surface by dipping clean rice into betaglucan solution or spraying betaglucan solution on the rice. After application, the rice is dried in a room temperature or hot air dryer to produce rice containing beta glucan.
본 발명은 알칼리 용액에 베타글루칸을 용해시키고, 이 용액에 쌀을 침지시키거나 용액을 쌀에 분무시킴으로써 베타글루칸을 함유한 쌀을 생산하는 방법을 제공한다.The present invention provides a method of producing beta glucan-containing rice by dissolving beta glucan in an alkaline solution and immersing the rice in this solution or spraying the solution into the rice.
본 발명은 알칼리 용액으로서 수산화나트륨, 수산화칼륨, 암모니아수, 인산3칼륨, 인산3나트륨으로 이루어진 그룹 중 1종 이상을 선택하는 것을 특징으로 하는 베타글루칸을 함유한 쌀을 생산하는 방법을 제공한다.The present invention provides a method of producing a beta glucan-containing rice, characterized in that at least one selected from the group consisting of sodium hydroxide, potassium hydroxide, aqueous ammonia, tripotassium phosphate, trisodium phosphate as the alkaline solution.
또한, 본 발명은 침지 또는 분무 횟수를 반복함으로써 베타글루칸 함량을 증가시키는 것을 특징으로 하는 베타글루칸을 함유한 쌀을 생산하는 방법을 제공한다.The present invention also provides a method of producing beta-glucan-containing rice, characterized in that by increasing the number of beta glucan by repeating the number of dipping or spraying.
본 발명은 알칼리 용액의 농도를 0.01∼5중량%로 하는 것을 특징으로 하는 베타글루칸을 함유한 쌀을 생산하는 방법을 제공한다.The present invention provides a method for producing rice containing beta glucan, wherein the concentration of the alkaline solution is 0.01 to 5% by weight.
뿐만 아니라, 본 발명은 베타글루칸이 0.1 ~ 5.0중량% 농도로 함유되는 것을 특징으로 한다.In addition, the present invention is characterized in that the beta glucan is contained in a concentration of 0.1 to 5.0% by weight.
나아가, 본 발명은 베타글루칸의 코팅 능력을 이용하여 미네랄 또는 생리활성 물질을 첨가하는 것을 특징으로 하는 베타글루칸을 함유한 쌀을 생산하는 방법을 제공한다.Furthermore, the present invention provides a method for producing beta-glucan-containing rice, characterized in that the addition of minerals or bioactive substances using the coating ability of the beta-glucan.
이하, 실시예에 의하여 본 발명을 상세히 설명한다. 그러나, 하기 실시예는 본 발명을 구체적으로 예시하는 것일 뿐, 본 발명의 내용이 실시예의 기재에 의해 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by way of examples. However, the following examples merely illustrate the present invention in detail, and the content of the present invention is not limited by the description of the examples.
실험예: 성분분석 방법Experimental Example: Component Analysis Method
베타글루칸으로 코팅한 쌀의 성분분석을 위하여, 코팅된 쌀 100g을 분쇄기로 0.2 ㎛ 이하로 분쇄하고, 60℃ 이상의 열수로 용출하여 원심분리한 후 상등액을 하기의 방법으로 각각 정량하였다.In order to analyze the composition of the rice coated with beta glucan, 100 g of the coated rice was pulverized to 0.2 μm or less with a grinder, eluted with hot water of 60 ° C. or higher, and centrifuged, and the supernatant was quantified in the following manner.
1) 베타글루칸: 시료에 0.5%의 NaOH를 첨가하여 용해시킨 후 적절히 희석하였다. 희석된 시료를 베타글루칸 정량용 BGstar kit(마루하 상사, 일본)의 반응시약과 30분간 반응시킨 후 발색시약을 넣고 첨가하여 분광흡도계를 사용하여 측정하였다.1) Beta-glucan: The sample was dissolved by adding 0.5% NaOH and then diluted appropriately. The diluted sample was reacted with the reaction reagent of the beta glucan BGstar kit (Maruha Corp., Japan) for 30 minutes, and then the coloring reagent was added and measured using a spectrophotometer.
2) 양이온: 유도결합플라즈마 원자방출기 (ICPS-1000III, Shimadzu Corp.)를 사용하여 이온 분석을 하였다.2) Cation: Ion analysis was performed using an inductively coupled plasma atom emitter (ICPS-1000III, Shimadzu Corp.).
3) 인산: 인산(phosphate) 농도가 20 mg/ℓ이하가 되도록 시료 0.5 ㎖와 반응시약 1.5㎖를 섞어서 37℃에서 120분동안 반응시킨 후 분광흡도기를 사용하여 820nm에서 흡광도를 측정하였다. 이때 반응 시약은 아스코빅산과 암모니움 몰리브데이트, 황산으로 만든다.3) Phosphoric acid: 0.5 ml of the sample and 1.5 ml of the reaction reagent were mixed so that the concentration of phosphate was 20 mg / l or less and reacted at 37 ° C. for 120 minutes, and the absorbance was measured at 820 nm using a spectroscopic absorber. The reaction reagent is made of ascorbic acid, ammonium molybdate, sulfuric acid.
4) 암모니움: 시료 400㎕와 반응액 A 400㎕, 반응액 B 200㎕, 반응액 C 400 ㎕를 순서대로 섞어 37℃에서 120분 반응시킨 후 흡광분도기를 이용하여 630nm에서 흡광도를 측정하였다. 반응액 A는 니트로푸루사이드 염으로 이루어지며, 반응액 B는 KOH(50g/L), 그리고 반응액 C는 K2CO3와 하이포클로라이드염으로 구성된다.4) Ammonium: 400 μl of the sample, 400 μl of the reaction solution A, 200 μl of the reaction solution B, and 400 μl of the reaction solution C were reacted for 120 minutes at 37 ° C., and then the absorbance was measured at 630 nm using an absorbance spectrometer. A reaction solution is made up of side-nitro puru salt reaction solution B, KOH (50g / L), and the reaction solution C is of a K 2 CO 3 and the hypo-chloride salt.
5) 카르니틴: 카르니틴 아세틸트랜스퍼레이즈(시그마, 미국)를 이용한 효소측정법을 이용하여 정량하였다.5) Carnitine: Quantitatively determined by enzymatic assay using carnitine acetyltransferase (Sigma, USA).
실시예 1. 인산3칼륨 베타글루칸 용액에 쌀을 침지시켜 글루칸 함유 쌀을 생산하는 방법Example 1 Method of Producing Glucan-Containing Rice by Soaking Rice in Tripotassium Phosphate Beta-Glucan Solution
먼저, 본 발명에 사용된 베타글루칸은 애그로박테리움에 설탕을 탄소원으로 사용하고, 염화암모늄을 질소원으로, 그리고 기타 무기물을 포함하는 발효배지에서 배양하여 생산하였다. 발효가 완료된 후 베타글루칸은 불용성 상태로 존재한다. 베타글루칸은 중성의 용액상태에서는 녹지 않기 때문에 발효액 중의 베타글루칸을 세포와 분리하기 위해 배양액을 염기화시켜 베타글루칸을 용해시킨 후 원심분리 또는 필터에 의해 세포를 제거한다. 이 용액을 수용성 유기용매와 접촉시키거나 용액을 중성화시킴으로써 베타글루칸을 침전시킨 후 원심분리하고 이것을 건조시켜 정제된 분말을 얻는다. 베타글루칸은 위와 같이 정제되거나 정제되지 않은 형태로도 사용될 수 있다. 본 발명의 실시예에서는 상기 방법으로 정제된 분말형을 사용하였다.First, beta glucan used in the present invention was produced by culturing in a fermentation broth containing sugar as a carbon source, ammonium chloride as a nitrogen source, and other minerals in Agrobacterium. After fermentation is complete, beta glucan is in an insoluble state. Since beta glucan is not dissolved in the neutral solution state, in order to separate the beta glucan from the fermentation broth with the cells, the medium is lysed to dissolve the beta glucan, and then the cells are removed by centrifugation or a filter. Beta glucan is precipitated by contacting this solution with a water-soluble organic solvent or neutralizing the solution, followed by centrifugation and drying to obtain a purified powder. Beta glucan can also be used in purified or unpurified form as above. In the embodiment of the present invention was used a powder form purified by the above method.
본 발명은 베타글루칸을 함유한 쌀을 제조하기 위하여, 상기 분말상 베타글루칸을 인산3칼륨 용액에 용해시켰다. 인산3칼륨 0.4중량% 1리터에 베타글루칸을 1.5중량%가 되도록 용해시킨 후, 청량미 500g을 거즈에 싼 다음 인간3칼륨 베타글루칸 용액에 침지하였다. 베타글루칸이 잘 스며들도록 천천히 흔들어 주면서 2 내지 3시간 경과 후, 꺼내어 무게가 변하지 않을 때까지 자연풍으로 건조하였다. 건조 후 베타글루칸 함량과 인산 그리고 칼륨을 정량하였다 (표1).In the present invention, in order to prepare a rice containing beta glucan, the powdered beta glucan was dissolved in a tripotassium phosphate solution. After dissolving beta glucan to 1.5% by weight in 0.4% by weight of tripotassium phosphate, 500 g of fresh rice was wrapped in gauze and then immersed in a solution of human tripotassium betaglucan. After slowly shaking the beta glucan soaking well, after 2-3 hours, it was taken out and dried in a natural wind until the weight did not change. Beta-glucan content, phosphoric acid and potassium were determined after drying (Table 1).
실시예 2. 인산3칼륨에 용해시킨 베타글루칸 용액을 쌀에 분무하여 글루칸 함유 쌀을 생산하는 방법Example 2 Method of Producing Glucan-Containing Rice by Spraying Beta Glucan Solution Dissolved in Tripotassium Phosphate to Rice
실시예 1과 동일한 방법으로 베타글루칸 용액을 제조하여 평판에 쌀을 고르게 분산시키고, 그 위에 상기 실시예 1과 동일한 글루칸 용액을 분무하여 베타글루칸을 코팅하였다. 건조 후 글루칸 함량과 인산 그리고 칼륨을 정량하였다 (표1).A beta glucan solution was prepared in the same manner as in Example 1, and rice was evenly dispersed on the plate, and the same glucan solution was sprayed on the plate to coat the beta glucan. After drying, the glucan content, phosphoric acid and potassium were quantified (Table 1).
실시예 3. 인산3칼륨에 베타글루칸을 용해시킨 후 다시 중화시킨 용액에 쌀을 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 3 Method of Producing Beta-glucan-Containing Rice by Dissolving Beta-Glucan in Tripotassium Phosphate and Dipping Rice in Neutralized Solution
실시예 1과 동일한 방법으로 인산3칼륨에 베타글루칸을 용해시키고, 다시 0.4%의 인산1칼륨을 첨가하여 중화시켜 젤 상의 용액을 만든다. 실시예 1과 동일한 방법으로 쌀을 침지하고, 건조 후 베타글루칸 함량과 인산 그리고 칼륨을 정량하였다 (표1).Betaglucan was dissolved in tripotassium phosphate in the same manner as in Example 1, and neutralized by addition of 0.4% potassium monophosphate to form a solution on a gel. The rice was immersed in the same manner as in Example 1, and the beta glucan content, phosphoric acid and potassium were quantified after drying (Table 1).
실시예 4. 베타글루칸 용액에 쌀을 반복해서 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 4 Method of Producing Beta-glucan-Containing Rice by Repeated Dipping of Rice in Beta-Glucan Solution
실시예 1과 동일한 방법으로 베타글루칸을 용액을 제조한 후, 쌀을 침지하고 적당히 말린 후 다시 침지함으로써 쌀의 베타글루칸 함량을 높였다. 총 3회 침지 후 건조하여 베타글루칸, 인산, 칼륨을 정량하였다 (표1).After preparing a solution of beta glucan in the same manner as in Example 1, the beta glucan content of the rice was increased by immersing the rice, drying it properly, and immersing it again. After total immersion three times, dried to quantify beta glucan, phosphoric acid, potassium (Table 1).
실시예 5. 암모니아수에 베타글루칸을 용해시킨 후 쌀을 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 5 Method of Producing Beta-Glucan-Containing Rice by Dissolving Beta-Glucan in Ammonia Water
암모니아수 5중량%에 1.5중량%의 베타글루칸을 용해시킨 후, 실시예 1과 동일한 방법으로 베타글루칸을 용액에 쌀을 침지하고 건조 후 베타글루칸 함량과 암모니움을 정량하였다(표1).After dissolving 1.5% by weight of beta glucan in 5% by weight of ammonia water, beta-glucan was immersed in a solution in the same manner as in Example 1, and the beta-glucan content and ammonium were quantified after drying (Table 1).
실시예 6. 인산3칼륨과 인산3나트륨 혼합액에 베타글루칸을 용해시킨 후 쌀을 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 6 Method of Producing Beta-glucan-Containing Rice by Dissolving Beta Glucan in Tripotassium Phosphate and Trisodium Phosphate Mixture
각 0.2중량%의 인산3칼륨과 인산3나트륨 1리터에 베타글루칸을 1.5중량%가 되도록 용해시킨 후, 실시예 1과 동일한 방법으로 베타글루칸 용액에 쌀을 침지하고 건조시킨 후 베타글루칸, 인산, 칼륨, 나트륨을 정량하였다 (표1).After dissolving beta glucan to 1.5% by weight in each 0.2% by weight of tripotassium phosphate and 1 liter of trisodium phosphate, immerse and dry rice in betaglucan solution in the same manner as in Example 1, and then betaglucan, phosphoric acid, Potassium and sodium were quantified (Table 1).
실시예 7. 베타글루칸 용액에 칼슘카보네이트를 첨가한 후 쌀을 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 7 Method of Producing Beta-Glucan-Containing Rice by Adding Calcium Carbonate to Beta-Glucan Solution
실시예 1과 동일한 방법으로 인산3칼륨에 베타글루칸을 용해시키고, 0.1%의칼슘카보네이트를 첨가하여 현탁액을 제조하였다. 실시예 1과 동일한 방법으로 쌀을 침지하고, 건조 후 베타글루칸 함량과 인산, 칼륨, 칼슘을 정량하였다 (표1).Betaglucan was dissolved in tripotassium phosphate in the same manner as in Example 1, and a suspension was prepared by adding 0.1% calcium carbonate. Rice was immersed in the same manner as in Example 1, and after drying, beta glucan content and phosphoric acid, potassium, and calcium were quantified (Table 1).
실시예 8. 베타글루칸 용액에 카르니틴을 첨가한 후 쌀을 침지하여 베타글루칸 함유 쌀을 생산하는 방법Example 8 Method of Producing Beta-glucan-Containing Rice by Adding Carnitine to Beta-Glucan Solution
실시예 1과 동일한 방법으로 인산3칼륨에 베타글루칸을 용해시키고, 지방대사에 중요한 역할을 하는 카르니틴을 1중량%가 되도록 첨가하여 현탁액을 제조하였다. 실시예 1과 동일한 방법으로 쌀을 침지하고, 건조 후 베타글루칸 함량과 인산 , 칼륨을 정량하였다(표 1).A suspension was prepared by dissolving beta glucan in tripotassium phosphate in the same manner as in Example 1 and adding carnitine to 1% by weight, which plays an important role in fat metabolism. Rice was immersed in the same manner as in Example 1, and after drying, the content of beta glucan, phosphate, and potassium was quantified (Table 1).
(단위: 중량%)(Unit: weight%)
본 발명은 물에 불용성인 베타글루칸을 알칼리 용액에 녹인 후 침지 또는 분무 방법에 의하여 쌀에 베타글루칸 및 미네랄을 도포함으로써, 글루칸 함량이 0.1% 이상 되는 기능성 쌀을 제조할 수 있었다. 이는 베타글루칸이 가지는 면역증강능을 부가된 쌀로서, 이러한 기능성 쌀을 섭취함으로써 현대인의 영양불균형 및 외부환경에의 적응력을 강화할 수 있는 효과가 있다. 또한, 베타글루칸의 코팅력을 이용하여 다양한 생리활성 물질을 쌀에 부가할 수 있다.The present invention was able to prepare functional rice having a glucan content of 0.1% or more by dissolving beta glucan insoluble in water in an alkaline solution and then applying beta glucan and minerals to rice by dipping or spraying. This is a rice added to the immune enhancing ability of beta glucan, by ingesting such functional rice has the effect of enhancing the nutritional imbalance and adaptability to the external environment of modern people. In addition, various bioactive substances may be added to rice using the coating power of beta glucan.
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| KR20010094102A (en) * | 2000-04-04 | 2001-10-31 | 이대순 | Processing of barley β-glucan enriched grain |
| KR20020037902A (en) * | 2000-11-16 | 2002-05-23 | 장철호 | A manufacturing method of barley β-glucan enriched grain including natural substance |
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