KR100454913B1 - Methods for identification of Korean wild mountain ginseng - Google Patents

Abstract

The present invention relates to a method for discriminating Korean wild ginseng, using a small amount of Korean wild ginseng and Chinese wild ginseng samples to perform morphological characterization, genetic characterization, proteomic characterization, and bioactive substance analysis. By comparing, it has an excellent effect of accurately discriminating Korean wild ginseng and Chinese wild ginseng.

Description

Method for identification of Korean wild mountain ginseng}

The present invention relates to a method for discriminating Korean wild ginseng. More specifically, the present invention relates to a method for determining Korean wild ginseng by analyzing the morphological characteristics, genetic characteristics, proteomic properties, and contained physiologically active substances of Korean wild ginseng.

Korean wild ginseng is a native of Panax ginseng C.A. It can be defined as ginseng belonging to a species called Meyer. Also, Korean wild ginseng can be divided into two types, wild ginseng and mountain ginseng. On the other hand, goats are classified as wild ginseng because they were cultivated wild in the mountains, and is also distinguished from wild ginseng because the first sowing is made by human hands. Goats ginseng in this sense can be classified into several types according to the initial seeding method. First, sowing seeds of wild ginseng in the mountains, second, harvesting the roots of wild ginseng from deep mountains, and transplanting them in the forest at the appropriate location; Most goat ginseng in circulation is originated in the third case. And goats are known to the general public as long brain ginseng (長 腦 蔘), morphologically in view of the general name given to the long brain part. However, in the present invention, the wild ginseng is defined by a general name referring to both natural wild ginseng and wild ginseng.

Since ancient times, wild ginseng has been developed by the experience of civilians and used for medicinal purposes and gradually depleted by excessive mining, and the growing method of ginseng began to develop. Cultivation of wild ginseng has begun to reach full scale. At the current government level, cultivated ginseng seeds are sown annually in mountainous areas across the country in the name of protecting plant resources. Especially, the private level is developing and activating cultivated wild ginseng.

The primary purpose of wild ginseng cultivation is to overcome the rare problem of wild ginseng, which is very important as a herbaceous plant, and its pharmacological efficacy and people's psychological effects are far less than natural wild ginseng, but they are recognized as a substitute. However, some wild ginseng growers are declining their value as wild ginseng by applying the general ginseng cultivation management method by fertilizer and pesticide treatment just like common ginseng. The wild ginseng cultivated as described above needs to be distinguished from the wild ginseng, which has grown for at least 10 years under natural conditions.

In recent years, as Chinese wild ginseng is smuggled into Korea in large quantities, it is confused with wild ginseng in Korea and is illegally distributed at a high price, which is emerging as a new social problem. Recently, there is a view that the origin of Chinese ginseng is likely to be cultivated ginseng seeds originating from Korea, and it is known that wider cultivation conditions are established without falling behind in cultivation technology or geographical condition. Under these conditions, Chinese wild ginseng grown in large quantities, such as cultivated ginseng, can be easily purchased by smugglers at a price much lower than domestic wild ginseng. It is occurring. The bigger problem is that until now, there has been little way to determine scientifically and reliably if it is illegally distributed in Korea. In particular, when genetically having the same source, a method of investigating by gene cloning, which has been recently developed, has a limit in determining a mountain.

As mentioned above, wild ginseng can be classified into many kinds according to the growing environment and its place of origin. However, since these are very mixed in the natural state or in the distribution process, wild ginseng having the highest quality grade of Korea's native is not accurately distributed to consumers. Is the reality. Therefore, in the face of these problems, the process of setting the grade between wild ginseng and scientifically and reliably, and determining the place of birth before being distributed to consumers is necessary.

The determination method of wild ginseng, which has been performed so far, has been mainly morphological analysis that does not damage the sample at all. This is because wild ginseng is very rare and is an expensive herb. However, morphological analysis has many difficulties in expecting scientific and reliable analysis results. Therefore, in recent years, there has been an example of extracting DNA from a relatively small amount of sample and attempting to determine the origin of cultivated ginseng by more scientific genetic analysis. However, if the seeds are of the same origin and differ only in their growing environment, genetic discrimination is also limited. Therefore, grading of wild ginseng by grading protein or other biologically active substance and production of mountain ginseng is essential as a more accurate and reliable determination method. However, current analytical techniques have a problem that many samples should be used when these various analytical methods are performed simultaneously.

In view of the above, the present inventors cut only a small portion of wild ginseng samples to be discriminated and performed various analytical methods simultaneously to develop a method for distinguishing Korean wild ginseng and Chinese wild ginseng, up to 50 mg or the like. Determination method to distinguish Korean wild ginseng and Chinese wild ginseng by examining the morphological, genetic and proteomic properties of Korean wild ginseng and Chinese wild ginseng using the following dry weights and analyzing and comparing the bioactive substances in each It was developed to complete the present invention.

Accordingly, an object of the present invention is to provide a method for determining Korean wild ginseng by examining and comparing molecular biological and physicochemical properties using a small amount of wild ginseng samples.

The object of the present invention is to observe Korean wild ginseng and Chinese wild ginseng with a digital camera and the naked eye and to weigh and compare the morphological characteristics, and to extract DNA from Korean wild ginseng and Chinese wild ginseng and genetically analyze it by RAPD method. Comparing by comparing the protein from Korean wild ginseng and Chinese wild ginseng, SDS-PAGE and 2D electrophoresis to examine the proteomic characteristics, and by comparing the bioactive substances contained in Korean wild ginseng and Chinese wild ginseng.

Hereinafter, the configuration of the present invention will be described.

Figure 1a is a photograph showing the appearance of the whole, fuselage, brain head, semi-section of Korean wild ginseng used as a sample in the present invention.

Figure 1b is a photograph showing the appearance of the whole, fuselage, brain head, semi-section of Chinese wild ginseng used as a sample in the present invention.

Figure 2 shows the results of RAPD analysis by PCR amplification using a primer after extracting and purifying the DNA of Korean wild ginseng (KM) and Chinese wild ginseng (CM) used as a sample in the present invention.

Figure 3 shows the results of comparing the content ratio of the panaxinol and panaxidol pattern and gas chromatography analysis of the methanol extract of Korean wild ginseng and Chinese wild ginseng used as a sample in the present invention.

Figure 4 shows the results of gas chromatography analysis of fatty acids of Korean wild ginseng and Chinese wild ginseng used as samples in the present invention.

Figure 5 shows the results of HPLC analysis of amino acids of Korean wild ginseng and Chinese wild ginseng used as samples in the present invention.

The present invention comprises the steps of analyzing the characteristics of the Chinese wild ginseng and Korean wild ginseng by measuring the weight and length after photographing the whole and the details of the Korean wild ginseng and Chinese wild ginseng by digital camera and observing the naked eye; Extracting DNA from Korean wild ginseng and Chinese wild ginseng, PCR amplifying and electrophoresis to analyze the characteristics of the Korean wild ginseng distinguished from Chinese wild ginseng by RAPD method to analyze DNA band differences; Extracting and quantifying proteins from Korean wild ginseng and Chinese wild ginseng, and analyzing the characteristics of Chinese wild ginseng and Korean wild ginseng by comparing protein patterns by SDS-PAGE and 2D gel electrophoresis; Methanol extracts of Korean wild ginseng and Chinese wild ginseng were analyzed by gas chromatography to compare peak patterns, and gas chromatography analysis of panaxydol and panaxynol showed anticancer activity. Analyze and compare the contents, gas-liquid chromatography analysis, qualitative and quantitative comparison of fatty acids contained in each wild ginseng methanol extract, HPLC and amino acid analysis by comparison, acidity by measurement of absorbance after carbazole color development Comparing the content of the polysaccharide, and analyzing and comparing the phenol component by gas-liquid chromatography.

Among the wild ginseng samples used in the present invention, Korean wild ginseng is exactly Korean Korean ginseng No. 1 to KM14, which is taken from Inje, Gangwon-do, and Chinese wild ginseng is also known as Chinese mountain ginseng No. 1 CM7) were collected in Yanbian, both of which have a age of about 15 years.

The present invention has ambiguity and limitations in applying the morphological discrimination method that has been performed in the past for the scientific and reliable discrimination between Korean wild ginseng and foreign wild ginseng, or the discrimination method by genetic analysis as a more scientific method. The problem is solved by further analyzing the protein pattern and analyzing various kinds of bioactive substances, and the precision of wild ginseng is determined through the four steps analysis of morphological, genetic, proteomic and bioactive substances. Maximizing the quality of the mountain, as well as the determination of the mountain has the advantage that can be accurately determined.

Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.

Example 1 Discrimination of Korean Wild Ginseng by Morphological Analysis

In the present embodiment, first, the whole photograph, fuselage photo, fuselage photo, semi photo and brain photo of Korean ginseng and Chinese ginseng are photographed from the front, rear, left and right directions using a digital camera. / Number, overall appearance, the degree of wrinkles of the fuselage, the color of the root, the overall and nodal form of the brain head, the number of the number of head. The length and diameter measurement tools were used to measure the overall length, the length and diameter of the fuselage, the length of the semi (the longest), the length and the diameter of the brain head, and the biomass.

As a result, Korean wild ginseng is shown in FIG. 1A, and Chinese wild ginseng is shown in FIG. 1B. Among the various analysis items, a comparative analysis result of the length ratio of the body to the maximum diameter of the body was 6.8, At least 5.0 and above, while the Chinese wild ginseng sample population was lower than the average value of 2.6 and the maximum of 3.7. Therefore, the criterion for domestic wild ginseng according to the ratio of the length of the fuselage to the maximum diameter of the fuselage could be determined at least 5.0. On the other hand, there was also a difference between the two sample groups in the number of semis. On the other hand, the domestic wild ginseng sample population averaged 7 and a maximum of 10, while the Chinese wild ginseng sample population averaged 18 and at least 12. Therefore, if the number of semi is less than 10, it can be said that the domestic wild ginseng.

Example 2: Korean wild ginseng determination by RAPD analysis

In this example, in order to perform RAPD analysis by extracting and purifying DNA from wild ginseng sample, a DNA extraction method modified from Draper and Scott's method was used. 10 mg of the lyophilized sample was ground with sand, and total DNA was extracted with 2 × CTAB buffer. After centrifugation, the supernatant containing DNA was purified 3-4 times with the same amount of phenol / chloroform solution. Only pure DNA particles were obtained by ethanol precipitation and centrifugation from the purified DNA extract. It was dissolved in 0.1 × TE buffer and RNase treated. Final DNA concentration and purity were measured by spectrophotometer. DNA amplification conditions and electrophoresis by PCR first, random primers used in PCR using a primer set consisting of 12 kinds of 20mers developed by SRILS (SRILS) It was. It is characterized by high reproducibility by annealing at 55 ° C. or higher and having a wide range applicable to various species. In this experiment, using 100 ng of primer and 50 to 200 ng of genomic DNA, the reaction was performed at 94 ° C for 4 minutes, 1 minute at 94 ° C, 1 minute at 57 ° C, and 2 at 72 ° C. The minute cycle was repeated 35 times, and then 7 minutes were further reacted at 72 ° C. After completion of the reaction, the product was electrophoresed on 1.5% agarose gel and stained with EtBr, and the DNA band difference was analyzed by an image analyzer (Biorad).

As a result, as shown in FIG. 2, domestic wild ginseng when RAPD (Random Amplified Polymorphic DNA) was performed using a primer having a nucleotide sequence of 5'-ATgTgTgCgATCAgTTgCTg-3 'among 12 kinds of primers DNA polymorphism has been identified to distinguish Chinese wild ginseng. 570 bp and 960 bp were DNA bands found only in Korean wild ginseng, and 1050 bp were DNA bands only found in Chinese wild ginseng.

According to the above results, the conventional RAPD method was recognized scientific value only by obtaining a DNA polymorphism result that can be statistically processed using a large number of random primers, but this method is generally a primer consisting of 8 to 10 nucleotide sequences. And the annealing temperature in the PCR reaction near 35 ℃ has a big problem in the reproducibility, and in the application of the above method to the distinction of wild ginseng, PCR reactions for many kinds of primers and the result There was the hassle of analyzing and statistics. Therefore, the primer (primer) used in the present invention is composed of 20 base sequences, the annealing (annealing) temperature is performed in the vicinity of 55 to 60 ℃ was very high reproducibility. Although RAPD analysis of one type of primer, considering the above facts, DNA bands that can distinguish domestic wild ginseng and Chinese wild ginseng can be used as RAPD markers.

Example 3: Korean Ginseng Discrimination by Proteomic Analysis of Two-Dimensional Electrophoresis

In this example, TCA solution A as a reagent is 10% trichloroacetic acid in acetone containing 0.07% 2-mercaptoethanol, and solution B is 0.07% 2-mecaptoethanol. 0.07% 2-mercaptoethanol in acetone containing 1 mM PMSF and 2 mM EDTA (0.07% 2-mercaptoethanol in acetone containing 1 mM PMSF and 2 mM EDTA) was prepared and extraction of total protein from wild ginseng sample was first lyophilized and then milled To 100 mg of the prepared sample, 100 µl of TCA solution A was added and suspended. The mixture was left at 45 DEG C for 45 minutes, centrifuged for 15 minutes, and the precipitate was suspended with 100 µl of solution B and left at 20 DEG C for 1 hour. The precipitate obtained by centrifugation was lyophilized, suspended in an electrofocusing solvent, stirred at 37 ° C. for 1 hour, centrifuged at 19000 g for 10 minutes, and the supernatant was subjected to protein quantification and 2D gel electrolysis. It was used for copper (2D gel electrophoresis).

Protein quantification was performed using a modified Bradford proteinassay method. After adding Coomassie brilliant blue G-250 and measuring the absorbance at 595 nm using a spectrophotometer, the measured value was used as a standard protein (ovalbumin). ) And the amount of protein in the sample was measured compared to the concentration standard line for globulin (globulin). SDS-PAGE is a method used for separation by molecular weight difference of proteins. The sample extract extracted by the protein extraction method is suspended in a buffer solution (10 mM Tris / HCl, 1 mM EDTA, pH 8.0), SDS 2.5%, After treatment with 5.0% β-mercaptoethanol and heating at 100 ° C. for 5 minutes, bromophenol blue was added 0.01%, insolubles were removed by centrifugation, and high salt concentration and low pH were good for the result. Dilution or desalting was performed and pH correction (below NaCl 0.75 M, pH 7-10, NaAc 0.35 M or more) was performed because of no adverse effect. Subsequently, electrophoresis was performed using 8-25% polyacrylamide gel (bisacrylamide: acrylamide = 1:29) (protein resolution, 6-150 kDa), and protein patterns of Korean ginseng and Chinese ginseng were compared.

2D gel electrophoresis is used for two-dimensional separation by protein molecular weight difference and isoelectric point difference. After protein extraction method, dilution or desalting is performed so that the salt concentration is 0.25 M and the concentration is adjusted by protein quantification (0.3- 0.5 ng / ul), followed by isoelectrofocusing using 5% polyacrylamide gel for IEF (protein resolution, 3-9 pI). After cutting one line with isoelectric focusing gel with scissors, place SDS-PAGE on the gel for SDS-PAGE horizontally and analyze the result by silver staining method. The protein pattern of was compared.

As a result, SDS-PAGE showed a clear band of 23 kDa in Korean wild ginseng samples, but not in Chinese wild ginseng samples. In case of 2D-PAGE, domestic wild ginseng showed a clear spot at 43 kDa and 5.6 pI, but not in Chinese wild ginseng. Therefore, Korean ginseng and Chinese ginseng could be distinguished more accurately. Therefore, 43 kDa, 5.6 pI protein was used as the determination scale protein of wild ginseng.

Example 4: Korean wild ginseng determination by physiologically active substance analysis

Process 1: Comparing GC Chromatogram Patterns

After freeze-drying the wild ginseng sample, 30 mg of crushed 30 mg was sonicated for 60 minutes with 0.6 mL MeOH, and after centrifugation, about 0.5 mL of the supernatant (methanol extract) was recovered, 100 μl of methanol extract was taken, and dried completely under nitrogen stream. 20 μl BSA (N, O-bis (trimethylsilyl) -acetamide) was added to perform TMS derivatization for GC analysis at 85 ° C. for 60 minutes. In the GC analysis conditions, the column is SE-54 capillary (30 m length × 0.25 mm ID, Alltech), detection temperature is 300 ℃, injection temperature is 260 ℃, column temperature and conditions from 100 ℃ → 4 ℃ / min → 290 ℃ (20 min), flow rate was 0.7 mL / min, split ratio was 1:50, and injection volume was 2 μl.

As a result, as shown in FIG. 3, the GC analysis of the methanol extract showed a total of 10 active ingredient peaks on a gas chromatogram, and a peak of 10 indicates a difference between domestic wild ginseng and Chinese wild ginseng. The average area percentage of peak 10 was 0.027%, which was at least 0.014%. On the other hand, in the Chinese wild ginseng sample population, the average area percentage was 0.015% and the maximum was 0.022%. Therefore, the criteria for the determination of domestic wild ginseng by GC pattern analysis was determined when the area percentage of peak 10 was at least 0.022%.

2nd process: content ratio analysis of panaxinol and panaxidol which are polyacetylene-type components

Panaxynol and panaxydol were simultaneously analyzed while comparing the GC patterns of the methanol extracts carried out in the first step.

As a result, as shown in FIG. 3, the content ratio of panaxynol to panaxydol was shown as a standard for distinguishing Korean wild ginseng and Chinese wild ginseng. The average content ratio of Korean wild ginseng sample population was 1.9 and at least 1.4, while the average content ratio of Chinese wild ginseng sample population was 1.0 and 1.3, respectively. Therefore, domestic wild ginseng according to the content ratio of panaxynol to panaxydol was determined to be at least 1.4.

Third Process: Fatty Acid Analysis

30 µl of methanol extract obtained from wild ginseng sample was taken, dried completely under nitrogen stream, mixed with 10 µl of BF3-MeOH, and subjected to methylation derivatization for 30 seconds in boiling water. EC-WAX capillary (30 m length × 0.32 mm ID, Alltech) was used for the GLC analysis.The detection temperature was 300 ° C, injection temperature 250 ° C, and column temperature was 100 ° C → 7 ° C / min → 220 ° C. (15 min), the flow rate was 1.8 mL / min and the partition ratio was 1:20.

As a result, as shown in Figure 4, a total of four types of fatty acid analysis of the methanol extract was identified. The ratio of saturated fatty acid palmitic acid and stearic acid to unsaturated fatty acid oleic acid and linoleic acid was 4.32 and standard deviation 2.89 for Korean wild ginseng sample population. In the case of Chinese wild ginseng sample population, the mean value was 5.1 and the standard deviation was 1.6. There were some differences, but they were not distinguishable. The lower the content ratio value, the higher the degree of unsaturation of fatty acids, which may be advantageous conditions in terms of pharmacological activity. Therefore, when the content ratio was 4.32 or less, it became a criterion to indirectly recognize the superiority as wild ginseng.

Fourth Process: Amino Acid Content Analysis

After extracting methanol from wild ginseng sample, 1 mL of H ₂ O was added to the remaining residue, and then ultrasonically crushed for 1 hour. After centrifugation, about 0.5 mL of the supernatant was filtered and used for analysis. 50 μl of MeOH extract and water extract (1: 1, v / v) were mixed and 100 μl of OPA emulsification solution was added, followed by reaction at room temperature for 1 minute and 100 μl of 0.1 M sodium acetate buffer. It was used for later analysis. The column used CUROSIL PFP (4.6 (250 mm, 5 micron, phenomenex) and the solvent condition was A solvent MeOH: THF: 0.02M sodium acetate (pH5.9) = 20: 2.5: 77.5, v / v and B solvent MeOH: THF: 0.02M sodium acetate (pH5.9) = 80: 2.5: 17.5, v / v The detection time, solvent A, solvent B and flow rate are summarized in Table 1. Injection amount was 10 μl, The fluorescence detector had Exitation λ = 360, Emission λ = 450 and the column temperature was 40 ° C.

Relationship between detection time, solvent A, solvent B and flow rate Minutes A solvent B solvent Flow rate 0 100 0 1 mL / min 2 100 0 1 mL / min 15 73 27 1 mL / min 35 0 100 1 mL / min 40 100 0 1 mL / min

As a result, aspartate, glutamate, serine, histidine, threonine, glycine, glycine, arginine, alanine, as shown in FIG. ), 13 kinds of free amino acids such as Methionine, Valine, Isoleusine, Leucine, and Lysine were analyzed. Methionine had a mean content of 0.27 ug / mg-dry weight and a minimum of 0.07 ug / mg-dry weight in Korean wild ginseng sample population, and an average content of 0.08 ug / mg-dry weight and a maximum of 0.14 ug in Chinese wild ginseng sample population. Isoleucine was 0.84 ug / mg-dry weight and at least 0.24 ug / mg-dry weight in Korean wild ginseng sample population and 0.2 in the wild ginseng sample population. The ug / mg-dry weight and the maximum 0.25 ug / mg-dry weight were investigated to distinguish the two sample groups. Therefore, the criteria for determination of Korean wild ginseng according to the content of free amino acid should be determined based on the case of more than 0.14 ug / mg-dry weight for methionine and more than 0.25 ug / mg-dry weight for isoleucine. Could.

Process 5: Acidic Polysaccharide Content Analysis

After extracting methanol from wild ginseng sample, 1 mL of H ₂ O was added to the remaining residue, followed by sonication for 1 hour, centrifugation, and about 0.5 mL of the supernatant was filtered and used for amino acid analysis. 1 mL of H ₂ O was added to the remaining residues and the supernatant, followed by extraction at 95 ° C. for 6 hours, and centrifugation. 1 mL of the supernatant obtained was mixed with 4 mL of EtOH to obtain a polysaccharide precipitate. After centrifugation, the supernatant was removed and re-dissolved in 1 mL of H ₂ O. Quantitative method after the sample 0.2 mL after the addition of 3 mL reaction in boiling water for 20 minutes and cooled to room temperature, 0.2 mL carbazole sol'n (0.15% in ethanol) - - cH 2 SO 4 (2-fold dilution of the stock solution 10) After the addition was completely mixed and reacted for 2 hours at room temperature and dark conditions, the absorbance was measured at 530 nm.

The results showed that the Korean wild ginseng sample population had an average content of 87 mg / g-dry weight and a minimum of 70 mg / g-dry weight, whereas the Chinese wild ginseng sample population had an average content of 62 mg / g-dry weight and a maximum of 79 The mg / g-dry weight was investigated. Therefore, the criteria for juvenile wild ginseng according to the content of acidic polysaccharides could be determined when the content is at least 70 mg / g-dry weight.

6th step: phenolic acid content analysis

Phenolic components bound to the free phenolic components and polysaccharides in the form of esters were individually extracted according to Krygier et al. In the analysis method, the extracted phenolic fraction was completely dried under a stream of nitrogen, and then 100 μl of BSA was added and reacted for 15 minutes in an 80 ° C. reactor to prepare a TMS derivative. TMS derivatized phenolic components were measured by gas-liquid chromatography using a FID detector, and the analytical column was SE-54 capillary (30 m length × 0.25 mm ID, Alltech). Was used. As the analysis conditions, the temperature of the injector and the detector was 260 ° C and 290 ° C, respectively, and the oven temperature was used to maintain a temperature of 4 ° C / min from 100 ° C to 260 ° C. Nitrogen was used as the moving gas and the split ratio was fixed at 1: 10 for analysis.

Experimental results showed that salicylic acid, cinnamic acid, p-hydroxybenzoic acid, vanillic acid, and gentis among the phenolic compounds identified in cultivated ginseng. Acid (gentisic), protocatechuic acid, syringic acid, p-coumaric acid, esculetin, ferulic acid, caffeic acid (caffeic acid) and the like were analyzed. As a result of analyzing the content of esculetin among the components, it was investigated as a reference component that can distinguish Korean wild ginseng and Chinese wild ginseng. Korean wild ginseng showed 47.2 ug / g-dry weight, while Chinese wild ginseng showed 30 ug / g-dry weight, which was higher in Korean wild ginseng.

As described above, the present invention compares the results of morphological characterization, genetic characterization, proteomic characterization and bioactive substance analysis of small amounts of Korean wild ginseng and Chinese wild ginseng samples. It is a very useful invention for the wild ginseng distribution industry because it has an excellent effect of accurately discriminating Korean ginseng and Chinese ginseng.

Claims (4)

The method of discriminating Korean wild ginseng, wherein the ratio of the length of the body to the maximum diameter of the body is 5.0 or more and the number of semis is 10 or less. delete In the electrophoretic method, SDS-PAGE to identify the band of 23kDa and 2D-PAGE to identify the spot of 43kDa, 5.6pl characterized in that the Korean wild ginseng. The area percentage of peak 10 by GC pattern analysis in the bioactive substance of the sample is 0.022% or more, the content ratio of panaxynol to panaxydol is 1.4 or more, and for unsaturated fatty acids (oleic acid and linoleic acid). The content ratio of saturated fatty acids (palmitic acid and stearic acid) is 4.32 or less, methionine is 0.14 µg / mg-dry weight or more, isoleucine 0.25 µg / mg-dry weight or more, and acidic polysaccharide 70 µg / g-dry weight Method for discriminating Korean wild ginseng, characterized in that the above or escurein contains more than 47.2㎛ / g-dry weight.