KR100449910B1 - Antimicrobial composition containing starfish extract - Google Patents

Abstract

The present invention relates to an antimicrobial composition containing an ethyl ether extract of starfish, and since the extract according to the present invention exhibits strong antibacterial activity against a wide range of bacteria, the antimicrobial composition containing the same has antibacterial activity. And as food compositions.

Description

Antibacterial composition containing starfish extract {ANTIMICROBIAL COMPOSITION CONTAINING STARFISH EXTRACT}

The present invention relates to an antibacterial composition containing an ethyl ether extract of starfish.

Starfish eat large value-added shellfish such as abalone and bottom-scattered scallops, causing great damage to the farms, as well as echinoderms such as mollusks such as oysters, small crustaceans, polypods, sea urchins, and starfish. It is a marine environment-destructing organism that adversely affects the ecosystem by dominating the ocean floor while eating.

Recently, efforts have been made to exterminate or remove starfish, which are marine environmental destructive organisms, such as using starfish as fertilizer or feed, or boiling or burning them off (Korean Patent Publication Nos. 84-722, 94-21476, 94 -21483 and Korean Patent No. 27638), these methods are very unproductive and cumbersome, the results have not been achieved.

Accordingly, the present inventors have found that the natural substance having strong antibacterial activity can be separated from the starfish through extraction with a specific solvent, and thus, the present invention has been completed.

It is an object of the present invention to provide an antibacterial composition containing a starfish extract.

1 shows an extraction schematic diagram of a solvent fraction using various solvents in Example 1. FIG.

In accordance with the above object, the present invention provides an antibacterial composition containing the extract obtained by treating the freeze dried starfish with ethyl ether at 10 to 40 ℃ for 30 to 120 minutes as an active ingredient.

Hereinafter, the present invention will be described in more detail.

Starfish to be used in the present invention include asbestos Terry or peck Tini Blow (Asterina pectinifera) as per starfish, asbestos Terry or batteries (A. batheri) and Aspergillus Terry or karini Blow (A. carinifera), Asterias as Amur starfish's no alkylene Four kinds of Asterias amurensis , and Henricia sp. , And extracts thereof are conventionally prepared by vacuum freezing dried starfish at 10 to 40 ° C, preferably at 20 to 25 ° C for 30 to 120 minutes. It can be prepared by treating with ethyl ether according to the phosphorus extraction method. At this time, if necessary, the extraction of ethyl ether can be repeated, and the obtained extract can be further purified separately or a pure ethyl ether fraction can be prepared through sequential solvent extraction of hexane and ethyl ether.

The extraction yield expressed as a percentage of the dry weight of the extracted material (ethyl ether soluble component) to the dry weight of the extract raw material (star starfish) obtained in the present invention is 4.0 to 8.0%, and the obtained extract is chromatographed to constitute As a result of identifying the structure of the separated components after pure separation of the components, the extract according to the present invention contains neoxanthin, astaxanthin, and the like as carotenoid pigments (fat components) as main components. .

Ethyl ether extract of starfish according to the present invention has a strong antimicrobial activity against a wide range of bacteria, including bacilli, such as E. coli and Bacillus subtilis , and Streptococcus aureus . Since the extract can be added to foods or the like as a food preservative. For example, the extract according to the present invention may be added to foods in an amount of 0.01 to 0.2% by weight, resulting in a food preservation effect.

In addition, the starfish extract according to the present invention does not show any toxicity, such as acute toxicity in the test using mice and does not show side effects on liver function.

Starfish extract of the present invention may be mixed with a suitable pharmaceutically acceptable carrier or excipient or diluted with a diluent according to a conventional method to prepare a pharmaceutical composition having an antimicrobial action. Examples of suitable carriers, excipients and diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, Microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. The pharmaceutical composition may further include fillers, anti-coagulants, lubricants, wetting agents, fragrances, emulsifiers, preservatives and the like. Pharmaceutical compositions of the invention can be formulated using methods well known in the art to provide rapid, sustained or delayed release of the active ingredient after administration to a mammal. The formulations may be in the form of tablets, pills, powders, sachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols, soft or hard gelatin capsules, sterile injectable solutions, sterile powders and the like.

The pharmaceutical compositions of the invention can be administered via several routes including oral, transdermal, subcutaneous, intravenous or intramuscular. Typical daily dosages of the starfish extract of the present invention range from 1 to 1,000 mg / kg body weight and may be administered once or in divided doses. However, it is to be understood that the actual dosage of the active ingredient should be determined in light of several relevant factors such as the route of administration, the age, sex and weight of the patient, and the severity of the patient, and therefore the dosage may be determined in any aspect of the invention. It does not limit the scope of.

The present invention also provides a food or beverage composition exhibiting an antimicrobial effect, comprising an effective amount of starfish extract. Examples of the food to which the starfish extract of the present invention may be added to exhibit an antimicrobial effect include various foods, beverages, gums, teas, vitamin complexes, and health supplements.

The starfish extract of the present invention may be added to the raw material during food preparation or properly mixed with the cooked food to prepare a food having an antimicrobial effect, in which case the content of the starfish extract in the finally prepared food or beverage is 0.001 to 10 Weight percent range.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only for illustrating the present invention, the present invention is not limited to these.

In addition, in the examples below, the following percentages for solids and solid mixtures, liquids and liquids, and liquids and solids are based on weight / weight, volume / volume and weight / volume, respectively, and all reactions are carried out at room temperature unless otherwise noted. It was.

Example 1: Determination of the antimicrobial activity of the extract according to the solvent

Starfish from the east and south coasts were collected as raw materials, and the ingredients were analyzed according to the AOAC method (Refer to Official methods of analysis, 15th ed., Association of Official Analytical Chemists, Arlington, 1990). Shown in

division Component Analysis Content (%) moisture Crude fat Crude protein View minutes Sugar East Coast Starfish 1.8 4.6 17.4 63.0 13.2 South Coast Starfish 1.8 2.6 20.3 64.8 10.5 * Ingredient analysis: moisture-atmospheric drying method, crude fat-Soxhlet method, crude protein-Kjeldahl method, ash-dry painting method, sugar- {100- (water + crude fat + crude protein + ash)} division Extraction yield by solvent (%) Hexane Ethyl ether Ethyl acetate Acetone Methanol water Hydrothermal East Coast Starfish Extract 0.45 5.25 0.08 0.23 13.7 2.08 1.01 South Coast Starfish Extract 0.4 5.06 0.86 0.15 5.03 0.43 1.36

division Escherichia coli Subtilis Staphylococcus 10 ^{* 1} 20 30 40 10 20 30 40 10 20 30 40 East Coast Starfish Extract Hexane - ^{* 2} - - - - - - - - - - - Ethyl ether +- + + ++ - - - - +- ++ ++ +++ Ethyl acetate - - - - - - +- + - - - - Acetone - - - - - - - - - - - - Methanol - - - - - - - - - - - - water - - - - - - - - - - - - Hydrothermal - - - - - - - - - - - - South Coast Starfish Extract Hexane - - - - - - - - - - - - Ethyl ether +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ +++ Ethyl acetate - +- + ++ - +- + ++ +- + ++ ++ Acetone - - - - - - - - - - - - Methanol - - - - - - - - - - - - water - - - - - - - - - - - - Hydrothermal - - - - - - - - - - - - * 1: Sample amount of extract (μl) * 2: Antimicrobial activity according to the size of the transparent ring (degree of growth inhibition):-8 mm or less, +-8 to 10 mm, + 11 to 15 mm, ++ 16 to 20 mm, + + 20 mm or more

From Table 3, it can be seen that there is a slight difference between the East and South coast starfish extract, but the antimicrobial activity is common in the ethyl ether extract fraction, and the weak antibacterial activity is also shown in the ethyl acetate extract fraction. In particular, in the starfish ethyl ether extract fraction of the south coast, a transparent ring of 20 mm or more was observed regardless of the concentration, and the antibacterial activity was confirmed to be the strongest.

division Growth inhibition minimum concentration (MIC, μg) Escherichia coli Subtilis Staphylococcus East Coast Starfish Extract Ethyl ether 3,935 - 2,625 Ethyl acetate - 1,600 - South Coast Starfish Extract Ethyl ether 443 255 271 Ethyl acetate 1,552 997 1,250

From Table 4, it can be seen that the ethyl ether extract fraction of the starfish shows a broad and strong antibacterial activity against bacteria. Example 2: Determination of the antimicrobial activity of the extract according to the starfish drying method

The east and south coast starfish were dried by different methods such as vacuum freeze drying, 25 ° C. cold air drying, and 40 ° C. hot air drying, and then extraction was performed in the same manner as in Example 1 using an ethyl ether extraction solvent. Subsequently, an antimicrobial test was performed according to the paper disk method shown in Example 1 using 40 μl of the extract sample having a concentration of 300 μg, and the results are shown in Table 5 below.

division Vacuum freeze drying 25 ℃ cold air drying 40 ℃ hot air drying Escherichia coli Subtilis Staphylococcus Escherichia coli Subtilis Staphylococcus Escherichia coli Subtilis Staphylococcus East Coast Starfish ++ ^* - +++ + - - - - - South Coast Starfish +++ +++ +++ +- - ++ + - - *: Antimicrobial activity according to the size of the transparent ring (degree of growth inhibition):-8mm or less, +-8-10mm, + 11-15mm, ++ 16-20mm, +++ 20mm or more

From Table 5, it can be seen that the antibacterial activity of the starfish raw material is lost not only by 40 ° C hot air drying but also by 25 ° C cold air drying. Example 3: effect of the extract according to the invention on food

division Viable cell count (cell / g) Save 1 day Save 3 days Save 5 days Control 1.1 × 10 ² 1.5 × 10 ³ > 10 ⁷ ether 1.3 × 10 ² 1.7 × 10 ³ > 10 ⁷ Starfish extract of the present invention 1.1 × 10 ² 3.0 × 10 ² 6.0 × 10 ³

division Viable cell count (cell / g) Save 1 day Save 3 days Save 5 days Control 2.0 × 10 ³ 3.2 × 10 ⁷ 2.8 × 10 ⁸ ether 5.0 × 10 ³ 1.4 × 10 ⁷ 2.2 × 10 ⁸ Starfish extract of the present invention 1.0 × 10 ¹ 2.2 × 10 ¹ 2.5 × 10 ²

As can be seen from Tables 6 and 7, the starfish extract of the present invention is added to food to bring excellent food preservation effect. Test Example: Oral toxicity test of the starfish extract of the present invention

20 females of 4 week old ISI (ICR) mice were bred for 1 week in an animal room with a temperature of 22 ± 3 ° C, a relative humidity of 50 ± 10%, and a roughness of 150-300 Lux. It was divided into four groups.

The ethyl ether extract fractions of the southern coast star star obtained from Example 1 were concentrated with a vacuum concentrator to remove the solvent, washed with water and dried. The obtained extract was dissolved in physiological saline and then orally administered to four groups of mice at doses of 300, 1,000 and 3,000 mg / kg, respectively. Changes in general symptoms and the presence of dead animals were observed for 7 days after administration. In addition, the mice were dissected and dissected at 7 days after the administration, and the internal organs were visually examined. The change in body weight was measured every day from the day of administration to observe the weight loss phenomenon of the animal by the starfish extract.

As a result, no toxicity was confirmed in all starfish extract-treated groups of 3,000 mg / kg or less. There was no gross findings in all the treated groups as a result of necropsy on living animals. In addition, no weight loss occurred in all dose groups. Preparation Example: Preparation of a pharmaceutical composition containing the starfish extract of the present invention

A pharmaceutical composition was prepared comprising the following ingredients.

200 mg of ethyl ether extract from starfish

Vitamin C 50 mg

Lactose (carrier) 150 mg

400 mg total

The ingredients were mixed well and filled into hard gelatin capsules to a total of 400 mg per capsule. By ingesting the prepared capsules 1 to 10 per day can bring about an antibacterial effect. In addition to vitamin C, the compositions may further include other healthful ingredients, and various oral preparations, such as tablets, granules, powders, microcapsules, drinks, suspensions, emulsions, syrups, and other liquids, according to conventional general preparations. It can be prepared as.

The ethyl ether extract of the starfish according to the present invention exhibits strong antibacterial activity against a wide range of bacteria, so that the antibacterial composition containing the same can be usefully used as a pharmaceutical and food composition having an antibacterial action. In addition, this application has the effect of producing high value-added natural materials from starfish, which are marine environmental destructive organisms.

Claims (3)

An antibacterial composition comprising an extract obtained by treating a vacuum starch dried starfish with ethyl ether at 10 to 40 ° C. for 30 to 120 minutes as an active ingredient. delete delete