KR100430383B1 - Method for manufaturing micro mixing channel device and method for fixing a tube on molded panel - Google Patents

Abstract

Disclosed are a method for manufacturing a mixed channel device including a panel member in which a fine channel circuit is engraved and a method for stably and easily fixing a tube on a panel formed by molding. In order to temporarily fix the tube on the molding plate, the magnet is adjacent to the molding plate, and then the magnet is used to fix the tube in which the magnetic structure is fixed to one side. In addition, a tube is fixed to the panel member by a tube fixing method using this magnet to produce a mixing channel device. This allows the tube to be fixed to the molding plate without damaging the molding plate and other structures, and the tube can be reliably mounted in the correct position.

Description

METHOD FOR MANUFATURING MICRO MIXING CHANNEL DEVICE AND METHOD FOR FIXING A TUBE ON MOLDED PANEL}

The present invention relates to a method for producing a mixing channel device for mixing liquids using a fine mixing channel and a method for fixing a tube on a panel. More specifically, the present invention provides a method for accurately and stably fixing a tube in an intended position to a panel manufactured by using a magnet, and the method using the magnet relates to a method for fixing a tube mounted on a mixing channel device. will be.

In order to fix a tube on a panel manufactured by molding, there is a method of forming a hole in the panel and inserting the tube into the hole to fix the tube with an adhesive. However, there is a disadvantage in that the use of the adhesive is cumbersome and the fluid leaks out. Moreover, in the case of a soft material such as silicone rubber, it is easy to break other tissues while forming holes directly, so that holes are not easily formed.

The mixed channel device refers to a device having a microchannel and mixing a plurality of materials, which is a kind of biochip.

Biochip is a biological micro-organism that can analyze gene expression patterns, gene defects, short distribution, and reaction patterns by combining biomolecules such as DNA and proteins on small substrates made of glass, silicon, nylon, etc. Biological Microchip. These biochips are attracting attention for revolutionary changes in science and technology research, new drug development processes, and clinical diagnosis, and are rapidly spreading. In particular, in the field of clinical diagnosis, biochips have been developed to detect and diagnose genetic mutations related to cancer and AIDS. On the other hand, the application of biochips has been made in agriculture, food, industry, and environmental monitoring.

Biochips are divided into Microarray and Microfluidics.

(Microfluidics) can be divided into chips. A microarray is a biochip that can arrange and attach thousands or tens of thousands of DNA or proteins at regular intervals, and process a binding material to analyze its binding pattern. A microfluidics chip is a Lab-on-a- It is also called chip (LOC), and it refers to a biochip that can analyze the reaction with various biomolecules or sensors concentrated on the chip while flowing a small amount of analyte. Recently, the microfluidics chip is expanding its field of separation, synthesis and quantitative analysis of analytes.

Microfluidics chip, or Lab-on-a-chip (LOC), is a condensation of two or more processes in a lab on a tiny chip, and the analysis of micromachining and microfluidics Say the equipment. It means to build a lab on a small chip like a semiconductor chip.

Conventionally, when using a cell culture plate (Cell Culture Plate) in the course of the experiment, it was common to fill the quantitation by hand to each well in the cell culture plate using a micro pipette. This method was a simple repetitive task and was not efficient in time or in manpower. Moreover, when the reagents to be administered at various concentrations need to be prepared for a specific purpose of experiments, since the error of the result is large due to the low skill of the experimenter, there has been a problem in the manufacture of experiments and reagents related to chemistry or biology.

In order to overcome this problem, there is a need for a device in which reagents necessary to be mixed at an appropriate concentration and automatically supplied to cells to be cultured in only the required amount are highlighted.

Indeed, microfluidics chips are used in areas related to toxicology assessments for drug development, medical and biochemical experiments, which include MEMS (Micro Electro Mechanical System) technology, Micro Fluidics and plastics. As a fusion of the Soft-Lithography technology, it has been mentioned as a base technology of Lab-on-a-chip (LOC) device along with DNA chip and protein chip.

Microchip technology not only flows within microchips, but also in applications where the results can vary significantly due to differences in micro-quantities, such as mixing of mixed cultures, toxicity tests according to the mixing ratio of poisons, biochemical experiments, and drug efficacy experiments. There are many improvements in the circuit design of chips and the development of devices using microchips.

It is therefore an object of the present invention to provide a method for accurately and stably fixing a tube in an intended position to a panel manufactured by magnets.

Another object of the present invention is to provide a method of manufacturing an apparatus for accurately mixing two or more fluids through a microchannel at a predetermined mixing ratio. In order to fabricate the panel member in which the channel circuit is engraved, the channel circuit is engraved by forming an embossed structure corresponding to the channel circuit in the molding plate which is the mold of the panel member and fixing the tube to the molding plate. It is possible to manufacture a mixing channel device is formed and the tube is fixed.

Another object of the present invention is to provide a method for stably fixing a tube in the process of molding a panel member in the process of manufacturing the mixing channel device.

Fixing the relief structure for plastic molding and the tube for fluid injection is essential to make a panel member out of plastic and to form a negative channel circuit in the panel member so that the fluid can pass through.

In the conventional method of fixing a tube or forming a groove in a molding plate by fixing the tube, the molding plate is damaged and the panel member is difficult to be separated in the process of fixing the molding plate or separating the molding plate from the panel member.

1 is a schematic diagram schematically showing an example of a mixed channel apparatus.

2 is a perspective view of the mixing channel arrangement shown in FIG.

3 is a perspective view illustrating a method of fixing a tube to a panel manufactured by molding according to an embodiment of the present invention.

4 is a perspective view illustrating a process after the panel member is manufactured by molding the molding plate illustrated in FIG. 3.

5 is a cross-sectional view illustrating a cross section of a magnetic structure in which a hole is formed at a center thereof.

6 is a perspective view for explaining a process of manufacturing a mixing channel device according to an embodiment of the present invention.

FIG. 7 is a cross-sectional view illustrating a cross section of X-X of FIG. 6 after molding the first panel member.

FIG. 8 is a perspective view illustrating the first panel member to which the tube illustrated in FIGS. 6 and 7 is fixed.

9 is a cross-sectional view illustrating a cross section of Y-Y of FIG. 8.

<Explanation of symbols for the main parts of the drawings>

100: mixing channel device 112,114: inflow member

122,124,126,128: Outflow member 130: Channel circuit

140: first channel unit group 150: second channel unit group

160: first panel member 165: second panel member

200,300: Molding plate 210,310: Magnet

220,320: Magnetic structure 230,330: Tube

340: embossed structure

According to a preferred embodiment of the present invention to achieve the above object of the present invention, a method of fixing a tube on a panel comprises the steps of: a) magnet adjacent to the back of the molding plate, b) at least one magnetic structure is fixed to one side One tube is provided, the magnetic structure being located on a molding plate, c) the panel is molded over the molding plate, and d) the magnet and the molding plate are separated from the panel.

In addition, a method of manufacturing a mixed channel device includes a) providing a molding plate having a shape corresponding to the channel circuit in relief, b) adjoining a magnet on a rear surface of the molding plate, c) at least one magnetic structure is fixed to one side One tube is provided, wherein the magnetic structure is positioned on the molding plate by a magnet, d) the first panel member is molded over the molding plate, and e) the magnet and the molding plate are separated from the first panel member. Include.

The mixing channel apparatus includes a first panel member, a second panel member in contact with the first panel member, a plurality of inflow members mounted on one side of the first panel member, at least one outlet member mounted on the other side of the first panel member, and And a channel circuit positioned between the first panel member and the second panel member and connecting the inflow member and the outflow member. The channel circuits used in the mixed channel apparatus have at least one channel unit, and the channel units are interconnected to constitute the channel circuit. Various channel circuits are configured according to the channel unit configuration and have respective characteristics.

The channel unit has a plurality of inlet channels, a cross chamber to which the inlet channels are connected, at least one micromix channel connected to the cross chamber and at least one outlet channel connected to the micromix channel.

FIG. 1 is a schematic diagram schematically showing an example of the mixed channel device 100, and FIG. 2 is a perspective view of the mixed channel device 100 shown in FIG. 1.

1 and 2, the mixing channel apparatus 100 includes a first panel member 160, two inlet members 112 and 114, four outlet members 122, 124, 126 and 128, and an inlet member 112 and 114 and an outlet member 122, 124, 126 and 128. Channel circuit 130 is connected.

The inflow members 112 and 114 have an injection tube 116, a hole formed in the first panel member 160, and an inflow reservoir. Tube 116 has a constant cross-sectional area regardless of the pressure within the tube, and the fluid flowing from the feeder (not shown) has a constant volume. Therefore, this embodiment is introduced according to the flow source method.

The channel circuit 130 includes a first channel unit group 140 and a second channel unit group 150 sequentially connected, and the first channel unit group 140 includes the fine channels 142 and 146 and the channel unit A. Are arranged in parallel, and the second channel unit group 150 arranges the fine channels 152 and 158 and the channel units B and C in parallel.

The microchannels 142, 146, 152, 158 and the channel units A, B, and C of both channel unit groups 140 and 150 are connected to other adjacent fine channels 142, 146, 152, 158 or the channel units A, B, and C, and the outlet members 122, 124, 126, 128 are connected. ), The fine channels 152 and 158 and the channel units B and C of the channel unit group 150 are connected to the outlet members 122, 124, 126 and 128, respectively.

The fine mixing channel 144 of the channel unit A has a cross-sectional area that is twice as large as that of the fine channels 142 and 146 positioned at both ends of the first channel unit group 140, and that of the channel units B and C. The fine mixing channels 154 and 156 have a cross-sectional area that is twice as large as that of the fine channels 152 and 158 positioned at both ends of the second channel unit group 150. In addition, the cross-sectional area of the microchannels 152 and 158 of the second channel unit group 150 has an area corresponding to 2/3 of the cross-sectional area of the microchannels 142 and 146 of the first channel unit group 140. Assuming that the length of the passage through which the introduced fluid passes through the outlet members 122, 124, 126, and 128 is the same, the resistance and the passage time of the channel circuit 130 may be kept the same by adjusting the cross-sectional area.

Thus, the two fluids that have passed through the mixing channel arrangement 100 shown in FIGS. 1 and 2 arrive at the same time at the outlet members 122, 124, 126, 128.

In addition, if a reagent such as a toxic substance is introduced into one inflow member 112 and a diluent material is introduced into the other inflow member 114, the mixing ratio of the mixed fluid passing through the microchannel and the micromixing channels 152, 154, 156, and 158 under the same conditions. Becomes 3: 2: 1: 0

The mixing channel apparatus of three or more stages may be configured by a method similar to the mixing channel apparatus 100 illustrated in FIGS. 1 and 2. Although proportional mixing ratio can be obtained as in the above embodiment, various mixing ratios can be obtained by adjusting the width, depth, or length of the channel.

The present invention also has the effect that it is possible to obtain a mixed fluid of various mixing ratios at once, but there is also an effect that a precise result can be obtained in one operation through the present invention the work made through two or more manual operations.

The first panel member 160 is made of a polymer material. For example, it refers to plastics such as polymethyl methacrylate (PMMA), polycarbonate, polytetrafluoroethylene (TEFLON ™), polyvinylchloride (PVC), polydimethylsiloxane (PDMS). Such a material is generally used for molding by molding as an inert material, and is preferable as a material for panel members due to its ease of manufacture, low cost, and one-off.

The channel circuit 130 is intaglio formed in the first panel member 160, and is formed intaglio by molding using a structure formed in relief on the molding plate corresponding to the channel circuit 130.

Outflow members 122, 121, 126, and 128 form reservoirs, and mixed material flows in and is stored in the reservoir. You can store the mixed material, but you can place the test or test object in the reservoir and then administer the mixed material to perform a direct test without any additional work.

A second surface on which channel circuit 130 is formed to seal two inlet members 112, 114, four outlet members 122, 124, 126, 128, and a channel circuit 130 connecting the inlet members 112, 114 and outlet members 122, 124, 126, 128. The panel member 165 is in contact. It is preferable to use glass as the second panel member 165.

According to an example of the mixing channel apparatus, a method of manufacturing the mixing channel apparatus 100 may include: a) providing a molding plate having an embossed shape corresponding to the channel circuit, and b) molding a first panel member on the molding plate. And c) mounting a plurality of inlet members and at least one outlet member to the first panel member.

The channel circuit in step a) includes at least one channel unit, the channel unit comprising a plurality of inlet channels, a cross chamber to which the inlet channels are connected, at least a micromix channel connected to the cross chamber and at least a micromix channel. It will have one outlet channel.

In order to form an intaglio channel circuit on one surface of the first panel member, an embossed structure formed corresponding to the channel circuit is formed on the molding plate and the first panel member is molded.

In order to mount the inlet member including the hole and the injection tube in step c), the hole is formed in the position where the tube is fixed and sealed, and then the tube is inserted into the hole and the adhesive member is fixed and sealed. Can be.

The outlet member may form a reservoir to store the mixed fluid, but the outlet member may be transferred to a desired place using a transfer member such as a tube.

The second panel member is superposedly coupled to one surface of the first panel member to seal the channels and / or chambers formed in the first panel member. For example, when the second panel member is made of glass, the second panel member may be bonded by heating to a temperature below the melting point of the polymer. However, the present invention is not limited thereto and may be coupled by various methods depending on the material of the second panel member and the first panel member.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited or restricted by the embodiments.

Example 1

The method of fixing a tube to a panel manufactured by molding includes a) a step in which a magnet is adjacent to a rear side of a molding plate, b) a tube having a magnetic structure fixed to one side, and a magnetic structure fixed to the tube by a magnet. Positioned on, c) molding the panel on the molding plate and the fixed tube, and d) separating the magnet and the molding plate from the molded panel.

3 is a perspective view illustrating a method of fixing a tube to a panel manufactured by molding according to an embodiment of the present invention.

Referring to FIG. 3, a tube having a molding plate 200, a magnet 210 adjacent to the molding plate 200, a magnetic structure 220 and a magnetic structure 220 positioned on the molding plate 200 fixed to one side thereof. 230 is shown.

The magnet 210 is adjacent to the rear side of the molding plate 200, and the magnet 230 generated by the magnet 210 and the tube 230 having the magnetic structure 220 fixed to one side are formed on the molding plate 200. Will be fixed on the

The magnetic structure 220 includes a base 222 and a tube receiving portion 224 protruding from one side of the base 222. The shape of the base 222 is preferably a shape and size sufficient to support the tube 230 and the magnetic structure 220 on the molding plate 200, the tube receiving portion protruded on one side of the base 222 224 may be inscribed inside the tube 230 to support the tube 230. In general, when fixing the circular tube 230, the tube receiving portion 224 is forcibly fitted inside the tube 230 in a cylindrical shape.

The magnetic body structure 220 is preferably using a structure made of iron, but if it is possible to form a magnetic material may be formed of a structure from another material.

4 is a perspective view illustrating a process after the panel member 240 is manufactured by molding the molding plate 200 illustrated in FIG. 3.

Referring to FIG. 4, the panel member 240 is molded and separated from the molding plate 200 while the magnetic structure 220 and the tube 230 are fixed.

After the magnet 210 and the molding plate 200 are separated from the panel member 240, the magnetic structure 220 is separated from the panel member 240 to remove the magnetic structure 220. The tube molded using the magnet 210 is excellent in fixing and sealing so that contents flowing into or out of the tube do not leak.

The magnet 210 may adjust the magnetic strength by physical distance using a permanent magnet, but may also adjust the magnetic force through the interruption of electricity using an electromagnet. In addition, the magnet 210 may be directly formed on one surface of the molding plate to directly fix the magnetic structure 220 or an embossed structure.

5 is a cross-sectional view illustrating a cross section of the magnetic structure 225 having a hole formed at the center thereof.

Referring to FIG. 5, a hole 228 may be formed in an axial direction connecting the base 226 of the magnetic structure 225 and the center of the tube receiving portion 227. When the hole 228 is formed, even if the tube is fixed on the panel, the magnetic structure is not limited even if the magnetic structure is not separated from the panel.

In addition, it is generally desirable to coat the outer and hole surfaces of the magnetic structure 228 with an inert material, such as plastic, to prevent chemical reaction of the magnetic structure 228 by the fluid passing through the tube.

Example 2

6 is a perspective view for explaining a process of manufacturing a mixing channel device according to an embodiment of the present invention, Figure 7 is a cross-sectional view showing a cross-sectional view of XX of Figure 6 after molding the first panel member 350. .

6 and 7, a molding plate 300 having an embossed structure 340, a magnet 310 adjacent to the rear surface of the molding plate 300, and a magnetic structure 320 positioned on the molding plate 300. And the magnetic structure 320 is shown on one side of the tube 330 is fixed.

A method for manufacturing a mixed channel device includes a) providing a molding plate 300 having an embossed structure 340 corresponding to a channel circuit, b) adjoining a magnet 310 to a rear surface of the molding plate 300. C) the magnetic structure 320 is provided on one side of the tube 330, the magnetic structure 320 is positioned on the molding plate 300 by the magnet 310, d) the molding plate Molding the first panel member 350 over the 300 and e) separating the magnet 310 and the molding plate 300 from the first panel member 350.

The relief structure 340 includes a location 342 in which an inflow reservoir is formed and the magnetic structure is fixed, an embossed shape 344 corresponding to the channel circuit, and an outflow reservoir mold 346 forming the outflow reservoir.

The embossed shape corresponding to the channel circuit is formed to have a width of about 0.1 mm and a height of 0.02 mm, but may be changed by the design of the channel, and the shape of the embossed structure 320 is not limited to the rectangle. 322 and the tube receiving portion 324 formed to protrude on one side of the base is preferably provided. The other side of the base 322 is fixed on the molding plate 300 by the magnet of the magnet 310, the first panel member 350 is molded, including the tube 330 fixed by the magnet 310. .

Holes may be formed in the axial direction between the base of the magnetic structure and the center of the tube receiving portion. If a hole is formed, the function of the tube is not limited even if the magnetic structure is not separated from the panel even after the tube is fixed on the panel.

After the magnet 310 and the molding plate 300 are separated from the first panel member 350, the magnetic structure 320 is also separated from the first panel member 350.

FIG. 8 is a perspective view illustrating the first panel member 350 to which the tube 330 illustrated in FIGS. 6 and 7 is fixed, and FIG. 9 is a cross-sectional view illustrating a cross section of Y-Y illustrated in FIG. 8.

8 and 9, two tubes 330 are fixed to the first panel member 350, and an inflow reservoir 352, a channel circuit, and an outflow reservoir are disposed on a bottom surface of the first panel member 350. Formed. The space formed by separating the magnetic body structure 320 becomes the inflow reservoir 352.

In addition, a second panel member (not shown) is overlapped and coupled to one surface of the first panel member 350 to seal the channel and / or the chamber formed in the first panel member 350. It is preferable to use a polymer material or glass as the second panel member.

According to the present invention, it is possible to facilitate the molding operation by stably fixing the tube to the panel to be molded by using a magnet, while also easy to disassemble the panel and the molding plate after production.

In addition, the stable molding can prevent the fluid or gas from leaking into or out of the tube.

In addition, in the case of fixing and molding a fine tube in relation to other biochip applications including a mixed channel device, it can be easily fixed and sealed using a magnet.

In addition, in the case of using a magnetic structure having a hole formed in the center, the work can be simply completed by fixing and molding the tube without a separate separation work.

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the killing and scope of the present invention described in the claims below. It will be understood that this can be changed.

Claims (13)

In the tube fixing method that one side of the tube is fixed to the panel to be molded, (a) adjoining the magnet on the rear of the molding plate; (b) providing at least one tube having a magnetic structure fixed to one side, the magnetic structure being located on the molding plate; (c) molding the panel on the molding plate; And (d) separating the magnet and the molding plate from the panel. The method of claim 1, In the step (b) the magnetic structure is provided with a base and a tube receiving portion formed to protrude on one side of the base and And the other side of the base is fixed on the molding plate by the magnetism of the magnet. The method of claim 1, And a hole is formed in the center of the magnetic structure in the step (b). The method of claim 3, wherein And the front surface of the magnetic body structure in which the hole is formed is coated with plastic and provided. The method of claim 1, The step (d) further comprises the step of separating the magnetic structure from the panel. In the method of manufacturing a mixed channel device, (a) providing a molding plate in which a shape corresponding to the channel circuit is embossed; (b) adjoining the magnet on the back of the molding plate (c) providing at least one tube with a magnetic structure fixed to one side, the magnetic structure being positioned on the molding plate by the magnet; (d) molding a first panel member on the molding plate; (e) separating the magnet and the molding plate from the first panel member. The method of claim 6, In the step (a), the channel circuit includes at least one channel unit, wherein the channel unit includes a plurality of inflow channels, a cross chamber to which the inflow channel is connected, at least one micromix channel connected to the cross chamber, and And providing at least one outlet channel connected to the fine mixing channel. The method of claim 6, In the step (c), the magnetic structure is provided with a base and a tube receiving portion formed to protrude on one side of the base and And the other side of the base is fixed on the first panel member by the magnetism of the magnet. The method of claim 6, The manufacturing method of the mixed channel device, characterized in that the (c) step further comprises a hole formed in the center of the magnetic structure. The method of claim 9, And the front surface of the magnetic body structure in which the hole is formed is coated with plastic and provided. The method of claim 6, The step (d) further comprises providing the outlet means comprising a reservoir connected to the channel circuit. The method of claim 6, The step (e) further comprises the step of removing the magnetic structure from the first panel member. The method of claim 6, And a second panel member contacting one surface of the first panel member having the channel circuit formed thereon.