KR100419710B1 - Novel trichosporon species and treatment of waste water or waste gas using same - Google Patents

Abstract

The present invention relates to a novel yeast strain and a method for treating wastewater or waste gas using the same, and immobilizes a new yeast strain Trichosporon loubieri Y1-A (KCTC-18079P) that decomposes hardly degradable and toxic chemicals. According to the wastewater or waste gas treatment method characterized by passing the waste water or waste gas through the carrier or filter, unlike the physical and chemical treatment methods requiring a large amount of energy and chemicals, Since the new yeast strain having excellent degradation ability is fixed to a carrier or a filter, the wastewater or waste gas can be easily and economically adjusted according to the on-site situation without changing complicated processing devices depending on the medium (water and air) passed through the filter or carrier. This can be done in an environmentally friendly way.

Description

Novel Trichosporon Yeast Strains and Wastewater or Waste Gas Treatment Methods Using the Same Yeast Strains {NOVEL TRICHOSPORON SPECIES AND TREATMENT OF WASTE WATER OR WASTE GAS USING SAME}

The present invention relates to a novel yeast strain and a method for treating wastewater or waste gas using the same, and more particularly, trichosporon loubieri Y1-A, which is a new yeast strain, is isolated from soil. The present invention relates to a technology and a process for stably decomposing toxic and hardly decomposable substances in waste gas or wastewater by using the ability to decompose difficultly decomposable chemicals.

Chemicals in waste gases and wastewater rely mainly on physical and chemical treatment methods because of their toxicity and hard degradability, which makes them difficult to treat in the prior art. Physical and chemical treatment methods require large amounts of energy and chemicals. As a result, it is very uneconomical and not environmentally friendly. In addition, even when biologically treating these wastewaters or waste gases, various types of substances that naturally adhere to the filter or media of the processing device while supplying nutrients for a certain period of time, rather than selecting and using microorganisms that are highly toxic and degradable. Due to the use of microorganisms, the treatment efficiency of high concentrations of toxic chemicals was lowered.

Therefore, there is a demand for a method that can economically and efficiently treat high concentrations of toxic and hardly decomposable chemicals contained in waste gas or waste water without adversely affecting the environment.

The present invention has been made in consideration of various disadvantages and problems associated with conventional treatment of toxic and hardly degradable chemicals, and an object thereof is to provide microorganisms having high viability and degradability for toxic chemicals directly from soil. will be.

Another object of the present invention is to provide a method capable of economically and efficiently treating toxic and non-degradable chemicals present in waste water or waste gas using the new microorganisms separated as described above.

1 is a schematic cross-sectional view showing the configuration of a wastewater treatment apparatus using a novel yeast strain Trichosporon loubieri Y1-A of the present invention,

Figure 2 is a schematic cross-sectional view showing the configuration of a waste gas treatment apparatus using a new yeast strain Trichosporon loubieri Y1-A of the present invention.

In order to achieve the above object, the present invention provides Trichosporon loubieri Y1-A (KCTC-18079P) that decomposes toxic and hardly decomposable chemicals.

Waste water or waste gas treatment method of the present invention for achieving the above another object, characterized in that passing the waste water or waste gas through a carrier or filter immobilized Trichosporon loubieri Y1-A (KCTC-18079P) strain It is done.

In the wastewater or waste gas treatment method of the present invention, the carrier may be made of at least one of polystyrene, polyurethane, polyether, polyethylene, polypropylene, and plastic, and the shape is preferably a granular shape having a diameter of 0.5 to 5 mm. . In addition, the filter may be made of at least one of polyester, polyurethane, polyethylene, plastic, sand and styrofoam, preferably having a porosity.

In the wastewater or waste gas treatment method of the present invention, the wastewater or waste gas is preferably mixed with air or oxygen and passed through a carrier or a filter.

In addition, in the wastewater or waste gas treatment method of the present invention, the washing liquid may be passed through the carrier or the filter in a direction opposite to the direction in which the wastewater or the waste gas passes through the carrier or filter, wherein the washing liquid is attached to the carrier or filter. It is preferable that the strain contains a component that can be used as a nutrient.

In the present invention, a new yeast strain was isolated from the contaminated soil by directly searching for microorganisms having high viability and degradation ability against toxic chemicals, and this was Trichosporon loubieri Y1-A (KCTC-18079P). Named. The novel yeast strains of the present invention can economically and efficiently treat toxic and non-degradable chemicals present in them when immobilized on a carrier or filter and passed through wastewater or waste gas.

Hereinafter, a novel yeast strain of the present invention and a wastewater or waste gas treatment method using the same will be described in detail.

1.Tricosporon rubies Trichosporon loubieri Separation and Properties of Y1-A)

In the present invention, a yeast strain having excellent degradability against hardly decomposable and toxic chemicals in waste water or waste gas was separated from soil of Yeosu, Jeonnam, and named as Trichosporon loubieri Y1-A. This strain was deposited with KCTC 18079P, Accession No. 26, 2001, to the Biotechnology Research Institute of Korea Institute of Science and Technology.

The strain was isolated according to the following procedure:

1 g of topsoil collected from Yeosu city was mixed well with 1 L of sterilized water, and then thoroughly mixed at a rotational speed of 1000 rpm using a magnetic stirrer. The sterilized water mixed with the soil was left to stand for about 10 to 30 minutes, and then 1 ml of the supernatant was mixed with 9 ml of the sterilized water prepared in advance, mixed well and diluted. 1 ml of the diluent was taken and mixed in a 50 ml liquid medium containing 0.2 to 1.0 g / l of phenol, prepared beforehand, as the sole carbon source. At this time, the liquid medium was maintained at a pH of about 6.5 using a buffer. The liquid medium was incubated in an incubator maintained at 30 ° C to find and isolate the bacteria formed in the liquid medium.

The novel yeast strain Trichosporon loubieri Y1-A of the present invention isolated by such a method formed a mycelia of the yarn-like microscopic observation (× 100) and formed a plurality of spherical asexual spores. It has a wide growth temperature from 8 to 40 ℃, it was confirmed that it can grow in the pH range of 4-9. In addition, it was possible to grow even in a high concentration range of 0.2 to 1.0 g / l phenol in a liquid medium in which phenol, a toxic chemical, was added as the only carbon source.

As a morphological characteristic of the cultures observed after culturing the novel yeast strain Trichosporon loubieri Y1-A in potato grapes sugar (PDA) medium for about 10 to 20 days, a thin thread-like mycelium It grows in concentric circles and grows closely covered with yeast spores between these hyphae, which is similar to the growth of ordinary bacteria. The overall color of the fungi growing on the PDA medium is pale ivory, and the light source can be seen that the fluffy mycelium is formed at the edge of the flora.

The present yeast strain Trichosporon loubieri Y1-A is a strain isolated for the first time in Korea according to the present invention, it is meaningful as a new strain completely different from the strain reported to be isolated in foreign countries. Compared to Trichosporon sp . , Which has been reported in foreign countries, this strain has not only about 5 to 20 times higher degradability than phenol, but also hardly degradable and toxic such as dyes and trichloroethylene in addition to phenol. It is a new strain that has excellent decomposition ability against chemicals.

Hereinafter, an apparatus and method for treating hardly decomposable and toxic chemicals in air and wastewater using the yeast strain Trichosporon loubieri Y1-A of the present invention will be described in detail.

2. Tricosporon rubies Trichosporon loubieri Wastewater Treatment with Y1-A)

1 is a schematic cross-sectional view showing the configuration of a wastewater treatment apparatus using a novel yeast strain Trichosporon loubieri Y1-A of the present invention.

The main body of the wastewater treatment apparatus consists of arranging a trichosporon loubieri Y1-A strain between the upper and lower strainers 2 in the cylindrical tank 1 in which the strain is fixed to the immobilization carrier 3. The upper part of the tank 1 is provided with a backwash water jet 4, which serves to spray backwash water transported by the backwash pump 5 from the upper part of the tank 1 to the lower part. In the lower part of the tank 1, the backwash water outflow pipe 10 through which the backwash water introduced from the upper part is discharged is installed. In addition, a wastewater inlet 6 is provided below the tank 1 so that the wastewater transported by the influent pump 7 and the air introduced through the air inlet tube 8 are mixed in the gas-liquid mixer 9 and then the tank ( 1) It is to be supplied from the bottom. In the upper part of the tank 1, the treated water outflow pipe 11 which discharges the wastewater processed while being supplied from the lower part and passing through the immobilization support | carrier 3 is provided.

The wastewater treatment apparatus having such a configuration operates as follows. That is, the wastewater that needs to be treated is mixed with air and then introduced from the bottom of the tank 1 through the wastewater inlet 6 to which the new yeast strain Trichosporon loubieri Y1-A of the present invention is attached. While passing between the carriers 3, the hardly decomposable and toxic chemicals in the wastewater are adsorbed to the strain or decomposed under aerobic conditions, and the treated wastewater is discharged through the treated water outlet pipe 10 installed above the tank 1. . On the other hand, the solids and sludge components formed in the tank 1 during the operation period of the apparatus is washed by the backwash water sprayed downward from the backwash water jet 4 installed above the tank 1 and together the tank 1 It is discharged through the backwash water outlet pipe 11 installed in the lower part.

The carrier 3 used to immobilize the strain of the present invention may be made of polystyrene, polyurethane, polyether, polyethylene, polypropylene, or plastics, and the shape of the carrier 3 may be granular. Although this is preferable, it is not specifically limited. In addition, when the size of the carrier is spherical, the specific surface area is preferably about 0.5 to 5 mm in diameter, but this is not particularly limited.

The shape and size of the tank 1 in the wastewater treatment apparatus are not particularly limited, and may be determined according to the load amount of the incoming wastewater and the pollutant properties. The material of the tank (1) is preferably a stainless steel system in order to prevent corrosion due to long-term use, considering the price and economics can be produced using a plastic or iron material.

The air supplied through the air inlet pipe (8) in the device can be obtained from the ambient atmosphere by using an air compressor (pure oxygen) if a high treatment efficiency is desired due to the characteristics of the strain attached to the carrier (3) May be used. The air and the pure oxygen introduced through the air inlet pipe 8 are mixed with the incoming wastewater and the gas-liquid mixer 9, and this gas-liquid mixer 9 may be the most efficient commercialized one.

In addition, when the backwash water is supplied to wash the solid components and sludge formed in the tank 1 in the wastewater treatment apparatus, the trichosporon loubieri Y1-A strain of the present invention can be used as a nutrient. It can be fed together by containing the ingredients, which can be made through the nutrient input tube (12). Nutrients are preferably included in the carbon component, nitrogen component, and inorganic salts used for the growth of Trichosporon loubieri Y1-A strain, but is not limited thereto. In this way, by supplying nutrients with backwashing water, it is possible to increase the activity of the strain attached to the carrier 3 inside the tank 1 and to enhance the treatment efficiency. It is good to use the backwash water jet 4 which is the most efficient among commercially available products.

3. Tricosporon rubies Trichosporon loubieri Waste gas treatment using Y1-A)

Figure 2 is a schematic cross-sectional view showing the configuration of a waste gas treatment apparatus using a novel yeast strain Trichosporon loubieri Y1-A of the present invention.

The main body of the waste gas treatment apparatus consists of arranging between the strainer 102 in the cylindrical tank 101 that the Trichosporon loubieri Y1-A strain is fixed to the immobilization filter 103. The upper portion of the tank 101 is provided with a nutrient solution injector 104 for injecting nutrient solution together with the strain, and serves to inject the nutrient solution transferred from the storage tank 105 together with the strain from the top of the tank 101. The lower portion of the tank 101 is provided with a filtrate outlet 108 so that the nutrient solution introduced from the upper portion is discharged or recovered to the storage tank 105 through the pump 107. In addition, a waste gas inlet 106 is provided in the lower portion of the tank 101 so that the waste gas mixed with air is supplied from the lower portion of the tank 101 to the upper portion. The treated gas is discharged through the purge gas discharge pipe 109 located above the tank 101.

The waste gas treatment device having such a configuration operates as follows. That is, contaminated waste gas requiring treatment is introduced from the bottom of the tank through the inlet port 106, and the novel yeast strain Trichosporon loubieri Y1-A of the present invention moves to the top of the apparatus due to the gas characteristics. Attached and fixed through the filter 103, wherein the decomposing action of the strain is difficult decomposition or toxic chemicals in the waste gas is adsorbed or decomposed to the strain and the discharge pipe 109 is installed on the tank 101, the purified gas Is discharged through). On the other hand, by spraying the nutrient solution downward with the strain through the nutrient solution injector 104 installed on the tank 101 during the operation period of the device, to increase the contact rate of the waste gas passing through the strain stuck to the filter 103 and the filter By increasing the activity and population of the strain formed in (103) it will increase the overall treatment efficiency. The nutrient solution is discharged through the filtrate outlet 108 under the tank 101 or recovered to the storage tank 105 through the pump 107.

The filter 103 used to immobilize the strain of the present invention may be made of a polyester, polyurethane, polyethylene, plastic, sand, styrofoam-based material, and is not particularly limited, and the growth and activity and adhesion efficiency of the strain Any material that can be used can be used. The shape of the filter 103 can be a rectangular parallelepiped or a cylinder according to the shape of the tank 101, and is produced so as to have a porosity. The filter 103 is located between the steel strainer 102 installed inside the device to minimize the movement of the nutrient solution and waste gas movement. In order to close the filter 103 to the inner wall of the tank 101 to minimize the gap between the inner wall and the filter 103, the size of the filter 103 is equal to the width of the inner wall of the tank 101.

The shape and size of the tank 101 in the waste gas treatment apparatus is not particularly limited, and may be determined according to the load amount of the incoming waste gas and the pollutant characteristics. The material of the tank 101 is preferably a stainless steel system in order to prevent corrosion due to long-term use, considering the price and economics, it can be manufactured using a plastic or iron material.

The nutrient solution sprayed from the nutrient solution injector 104 installed on the tank 101 of the waste gas treatment device is carbon and nitrogen components used for the growth of Trichosporon loubieri Y1-A strain, and Inorganic salts and the like are preferably included, but are not limited thereto. The nutrient solution injector 104 selects and uses the most effective product among those commercialized. A filtrate outlet 108 is installed below the tank 101 to periodically discharge the liquid and solid components generated or remaining during the operation period.

On the other hand, the waste gas flowing through the waste gas inlet 106 may be treated to include a volatile system hardly decomposable, toxic chemicals, wherein the trichosporon loubieri Y1-A strain of the present invention In order to increase the activity of the air is preferably supplied with pure oxygen.

Hereinafter, the wastewater or waste gas treatment method of the present invention will be described in more detail with reference to Examples. However, the following examples are only examples of the present invention and the present invention is not limited thereto.

Example 1

Using a wastewater treatment apparatus having the configuration as shown in Fig. 1, a tank having a capacity of 3L was filled with 2L of a polyethylene-shaped cube (1 cm × 1 cm × 1 cm) carrier and the tricosphorone ruby of the present invention. After attaching and growing the Echo ( Trichosporon loubieri Y1-A) strain, wastewater contaminated with carcinogenic 2,4-DCP (dichlorophenol, concentration: 400 mg / l) and dyeing plant wastewater (COD _Mn : 800 mg / l) ). The reaction time in the bioreactor was 16 hours, the concentration of the treated water was measured after reaching the steady state, and the average value and standard deviation of the treatment efficiency measured at 10-day intervals while operating the reactor were determined. . As a control, a microbial community naturally formed in the same wastewater treatment apparatus was used.

Table 1 shows the wastewater treatment efficiency of the Trichosporon loubieri Y1-A treatment group and the control group of the present invention. Wastewater from the dyeing plant was measured for COD _Mn concentration, and wastewater containing 2,4-chloride phenol was directly measured for concentration of 2,4-DCP using gas chromatography.

Treatment Efficiency of Dyeing Plant Wastewater Treatment efficiency of 2,4-chloride phenol-containing wastewater T. loubieri Y1-A group Control T. loubieri Y1-A group Control 88 ± 5% 24 ± 3% 94 ± 5% 12 ± 2%

As shown in the above results, the wastewater treatment method using the trichosporon loubieri Y1-A strain of the present invention shows a very high treatment efficiency compared to the control. In other words, due to toxicity and hardly degradable, it shows the possibility to treat 2,4-chloride phenol and dyeing plant wastewater which are difficult to treat biologically by the prior art very effectively.

Example 2

Using a waste gas treatment apparatus having the configuration as shown in FIG. 2 L of the tricosporon rubies of the present invention (Trichosporon loubieriY1-A) strains were attached and grown, and then trichloroethylene (TCE; trichloroethylene), known as a carcinogenic substance, was artificially adjusted to a concentration of 400 ppmv, and treatment efficiency was measured while passing through the apparatus. The gas flow rate was 30 ml / min, and as a control, a microbial community naturally formed in the same waste gas treatment apparatus was used.

Table 2 shows the waste gas treatment efficiency according to the operating time of the trichosporon loubieri Y1-A treatment group and the control group of the present invention. TCE concentration was measured using gas chromatography.

division Processing Efficiency According to Operating Time 5 days 10 days 15th 20 days 25 days 30 days T. loubieri Y1-A group 75% 69% 71% 72% 77% 73% Control 7% 6% 5% 7% 4 % 5%

As shown in the above results, the waste gas treatment method using the trichosporon loubieri Y1-A strain of the present invention showed a very high treatment efficiency compared to the control. In other words, due to toxicity and hardly degrading, it shows the possibility of treating trichloroethylene which is difficult to treat biologically with the prior art very effectively.

As described above, if the biological characteristics of the novel yeast strain Trichosporon loubieri (Y1-A) of the present invention is used in engineering, in the prior art to the toxicity and hardly degradable properties of the chemicals in the waste gas or wastewater By overcoming the limitations of biological treatments that result, it is possible to process these hardly degradable and toxic chemicals quickly and economically.

That is, the wastewater or waste gas treatment method of the present invention, unlike the physicochemical treatment method that requires a large amount of energy and chemicals, is a novel yeast strain tricosphoron with excellent degradation ability against hardly decomposable and toxic chemicals. Since the rubyeri ( Trichosporon loubieri Y1-A) is used to be fixed to a carrier or a filter, the wastewater or waste gas can be easily adapted to the site situation without changing complicated treatment devices depending on the medium (water and air) passed through the filter or carrier. It can be handled in an economic and environmentally friendly way.

Claims (7)

Trichosporon loubieri Y1-A (KCTC-18079P), which degrades toxic and hardly decomposable chemicals. Wastewater or waste gas treatment characterized by passing wastewater or waste gas through a filter having a granular carrier or porosity of 0.5 to 5 mm in diameter to which a trichosporon loubieri Y1-A (KCTC-18079P) strain is immobilized. Way. The method of claim 2, wherein the carrier is selected from the group consisting of polystyrene, polyurethane, polyether, polyethylene, polypropylene, and plastic, and has a granular shape with a diameter of 0.5 to 5 mm. The method of claim 2 wherein the filter is selected from the group consisting of polyester, polyurethane, polyethylene, plastic, sand and styrofoam, characterized in that it has a porosity. 3. The method of claim 2 wherein the wastewater or waste gas is mixed with air or oxygen to pass through a carrier or filter. The method of claim 2, wherein the washing liquid is passed through the carrier or filter in a direction opposite to the direction in which the waste water or waste gas passes through the carrier or filter. 7. The method according to claim 6, wherein the washing liquid contains a component which can be used as a nutrient by the strain attached to the carrier or the filter.