KR100415305B1 - Method for brewing wine by using germinated cereals - Google Patents

Method for brewing wine by using germinated cereals Download PDF

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KR100415305B1
KR100415305B1 KR10-2003-0025285A KR20030025285A KR100415305B1 KR 100415305 B1 KR100415305 B1 KR 100415305B1 KR 20030025285 A KR20030025285 A KR 20030025285A KR 100415305 B1 KR100415305 B1 KR 100415305B1
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germinated
fermentation
rice
corn
germination
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KR20030041115A (en
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권상일
김형식
이창수
정지용
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대한민국
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/021Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn

Abstract

본 발명은 벼, 옥수수, 밀을 발아ㆍ건조(發芽ㆍ乾燥)시킨 발아곡류를 원료로 발아곡류발효주(發芽穀類醱酵酒)를 제조하는 방법으로써, 발아벼, 발아옥수수, 발아밀을 분쇄하고 급수하여 1차 액화효소제(-Amylase)로 액화(液化)시킨 후 2차 당화효소제(-Amylase)로 당화(糖化)시켜 당화액(糖化液)을 얻은 다음 효모(酵母)를 첨가하여 알코올발효를 시키는 단행복발효법(單行複發酵法)에 의한 발아곡류발효주를 제조하는 방법에 관한 것이며, 본 발명의 방법에 의해 제조된 발아곡류발효주는 발아시 생성된 곡류효소와 아미노산 등 유효성분을 함유하며, 특히 발아ㆍ건조시 생성된 곡류특유의 고소하고 담백한 향기와 향미성분이 제성주에 부가되고, 발아시 생성된 자체효소와 부족분의 효소는 시판 액화ㆍ당화효소제로 보충하여 당화와 발효를 분리한 단행복발효방식으로 제조하므로써 입국(粒麴 : 곡류원료에 종균을 접종시킨 발효제), 또는 누룩 등 발효제에서 분비되는 구연산, 젖산등 강한 산취와 곰팡이취를 배제할 수 있어 기존 탁주 및 약주와는 전혀 주질이 다른 외관이 맑고 담백하며, 깨끗한 주질의 형태를 특징으로 하는 발아곡류발효주의 제조방법에 관한 것이다.The present invention is a method of producing germinated grain fermented wine from the germinated grains obtained by germinating and drying rice, corn and wheat, and grinding the germinated rice, germinated corn and germinated wheat. Primary liquefied enzyme Liquefied with -Amylase, followed by secondary glycosylase -A method for producing germinated grain fermented liquor by single fermentation fermentation method in which saccharified with Amylase to obtain saccharified liquid and then fermented with yeast for alcohol fermentation. , The germinated grain fermented wine prepared by the method of the present invention contains active ingredients such as grain enzymes and amino acids produced during germination, and in particular, the savory and light fragrance and flavor component unique to grains produced during germination and drying are added to the sake. The self-enzyme and deficiency enzyme produced during germination are supplemented with commercially available liquefaction / glycosylating agent and manufactured by a simple and single fermentation method that separates saccharification and fermentation (粒 麴: fermentation agent inoculated with spawn raw material) Strong acid and mold odors such as citric acid and lactic acid secreted from fermentation agents such as yeast can be excluded, so the appearance is completely different from existing Takju and Yakju. A method of producing germinating cereal fermented beverages, characterized in the form.

Description

발아곡류 이용한 주류 제조방법{Method for brewing wine by using germinated cereals}Method for manufacturing liquor using germinated grains {Method for brewing wine by using germinated cereals}

백미, 옥수수, 밀 등 전분질원료를 이용하여 탁주ㆍ약주 등 발효주를 제조할 경우 포도, 사과 등과 같은 당질원료와는 달리 전분을 당으로 분해시키는 당화공정(糖化工程)을 거쳐야 하며, 당화공정에 사용되는 발효제로는 주로 누룩(날곡류를 분쇄하여 적량의 수분을 가한 후 야생곰팡이 등의 사상균을 번식시킨 발효제의 일종)을 사용하거나 입국(곡류원료를 증자한 후 곰팡이류를 인위적으로 번식시킨 개량식 발효제의 일종)을 사용하고 있다.When producing fermented liquor such as Takju or Yakju using starch raw materials such as white rice, corn, wheat, etc., unlike sugar materials such as grapes and apples, they must go through saccharification process to decompose starch into sugar and used for saccharification process. The fermentation agent used is mainly Nuruk (a type of fermentation agent that breeds filamentous fungi such as wild fungus after grinding the grain grains and adds a proper amount of moisture) or enters (advanced fermentation agent that artificially breeds molds after increasing the raw material of grains). Kind of)

이러한 당화형 발효제에 대하여 문헌상 유래를 보면, 「지붕유설」에서는 일명 "미인주"라고 하여 16세정도의 아름다운 여인들이 곡물을 직접 씹어 빚은 술이 있었으며, 이와 같은 술은 우리나라뿐만 아니라 중국, 일본의 오끼나와에서도 찾아볼 수 있는데 이는 침속의 아밀라아제를 이용한 것이라 할 수 있으며, 중국의 「제민요술」에는 우리나라에서도 예전부터 술을 빚을 때 사용하던 누룩의 제조방법이 전해져 오고있어 예전부터 자연적으로 얻을 수 있는 곰팡이를 주류제조에 이용하였다는 사실을 알 수 있다. 또한 일본은 平安時代 초기인 9세기경 재래식 누룩과 함께 백미에 곰팡이를 인위적으로 배양시킨 개량식 발효제인 입국(粒麴)을 주류에 이용함으로써 주류제조에 획기적인 기술을 접목시켰다.According to the literature on the glycosylated fermentation agent, in the roof roofing snow, it was called "Beautiful rice wine" and a beautiful woman of 16 years old chewed the grain directly, and such alcohol was not only in Korea but also in China and Japan. It can also be found in the use of sedimentary amylase, and in China's `` Jimin Magic '', the manufacturing method of yeast used to make liquor has been transmitted in Korea. It can be seen that it was used to make liquor. In addition, Japan used innovative technology in liquor manufacturing by using immigration, an improved fermentation agent that artificially cultivated mold in white rice, along with conventional yeast in the early 9th century.

쌀, 소맥 등의 곡류에 야생곰팡이를 자연적으로 번식시키거나(누룩), 인위적으로 배양하여(입국) 곰팡이가 분비해 내는 효소를 이용하여 현행 탁ㆍ약주 제조방식의 발효주를 제조하는 경우 곰팡이가 생산하는 구연산과 젖산 등의 특이한 산미와 곰팡이취가 강하여 쌀, 소맥, 옥수수 등 전분질원료 특유의 맛과 향을 반감시킴과 동시에 주질이 농후하여 신세대들의 취향과는 점차 멀어져 가는 문제점이 있다.Mold is produced when fermented liquor of current turbidity and herbal medicine manufacturing method is produced using enzymes that naturally fertilize wild yeast in grains such as rice and wheat (yield), or artificially culture (entry) and secrete fungus. The unique acidity and mold odor such as citric acid and lactic acid are strong, and the taste and aroma of starch raw materials such as rice, wheat, and corn are halved, and the quality is rich, and there is a problem that gradually goes away from the taste of the new generation.

본 발명자들은 이러한 문제점을 없애기 위하여 완성한 발아곡류를 원료로한 발효주는 발아시 생성된 곡류효소를 주체로 하고 일부 부족분의 효소는 액화효소제(-Amylase)ㆍ당화효소제(-Amylase)로 보충하여 당화와 발효를 분리한 단행복발효방식으로 제조함으로써 발효제(누룩, 입국)를 사용하여 발효시키는 기존방법의 누룩, 입국 등 발효제에서 분비되는 구연산, 젖산 등 산미와 곰팡이취를 배제할 수 있어 기존 발효제를 이용한 탁주, 약주와는 주질이 전혀 다른 외관으로 맑고 담백하며, 깨끗한 주질의 발효주를 제조할 수 있고, 발아ㆍ건조시 생성된 곡류특유의 고소한 향기와 향미성분을 그대로 제품에 부가시킬 수 있어 곡류고유의 맛과 향을 잘 나타낼 수 있는 주류제조가 가능하며, 또한 발아시 생성되는 곡류효소와 비타민, 미네랄, 아미노산 등 각종 영양소가 부수적으로 제품에 이행됨으로써 젊은이들의 기호에 맞는 새로운 형태의 주류를 제조할 수 있다는 사실을 발견하고 발아곡류를 이용한 발효주를 발명하게 된 것이다.The inventors of the present invention to eliminate such problems, the fermented wine based on the finished germinated grains mainly based on the grain enzyme produced during germination, and some of the enzyme is liquefied enzyme ( Amylase) By supplementing with Amylase), it is manufactured by the simple fermentation method that separates the saccharification and fermentation. As it can be excluded, Takju and Yakju, which are different from the existing fermentation agents, are pure and pure in appearance, and fermented liquor can be manufactured. It is possible to make liquor that can express the unique taste and aroma of cereals, and various nutrients such as cereal enzymes, vitamins, minerals, amino acids, etc. produced during germination can be added to the product to meet the taste of young people. The discovery of a new type of liquor made it possible to invent fermented liquor using germinated grains.

도1은 본 발명에 따라 곡류원료를 발아ㆍ건조시킨 후 일정크기로 분쇄한 다음 급수 및 액화효소제(-Amylase)를 첨가하여 액화(호화 : 糊化)시킨 액화액(液化液)에 당화효소제(-Amylase)를 첨가하여 당화액(糖化液)을 완성한 다음 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 단행복발효 방식에 의하여 제조한 발효주에 대한 제조공정도이다.Figure 1 is after germination and drying of the grain raw material in accordance with the present invention and then crushed to a certain size water supply and liquefied enzyme ( -Amylase was added to the liquefaction solution (liquefying) This is a manufacturing process for fermented liquor prepared by single fermentation method by adding saccharomyces koreanus yeast culture solution to the saccharified solution by adding -Amylase.

날곡류(生穀類)인 벼, 옥수수, 밀을 약 15℃내외의 물에 48∼72시간 침지하여 발아에 필요한 수분을 충분히 흡수시킨 후 16∼22℃에서 6∼9일간 싹을 내어, 싹의 길이가 곡립의 1∼1.5배 정도가 되었을 때 발아를 중지시키고 50∼65℃의 온도에서 약 20∼24시간 건조한 후 정선ㆍ분쇄하여 발아벼, 발아옥수수, 발아밀을 얻은 다음, 발아곡류(발아벼, 발아옥수수, 발아밀 단용 또는 발아벼, 발아옥수수 1 : 1혼용) 1,000g당 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후, 액화액을 65℃로 냉각시킨 다음 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시키는 단행복발효법으로 발아곡류를 이용한 발효주를 제조하였다Raw grains of rice, corn, and wheat are immersed in water at about 15 ° C for 48-72 hours to absorb enough moisture for germination, and then shoots are made at 16-22 ° C for 6-9 days. When germination is 1 to 1.5 times the length of the grain, germination is stopped, dried for about 20 to 24 hours at a temperature of 50 to 65 ° C, and then selected and crushed to obtain germinated rice, germinated corn, and germinated wheat. Rice, germinated corn, germinated wheat for single or germinated rice, germinated corn for 1: 1 mix, water supply 2500 ml and liquefied enzyme -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. A fermented liquor using germinated grains was prepared by a single fermentation method in which fermentation was carried out for 4 to 5 days.

(실시예)(Example)

비교예 1: 생벼를 분쇄한 분쇄벼 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 9.2%의 벼발효주 2,200㎖를 제조하였다. Comparative Example 1 : 1,000 g of ground rice ground to 2,500 ml of water and liquefied enzyme ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. After fermentation for 4 to 5 days at 2,200ml of rice fermented liquor with 9.2% alcohol content was prepared.

실시예 1 :생벼를 약 15℃내외의 물에 48∼72시간 침지하여 발아에 필요한 수분을 충분히 흡수시킨 후 16∼22℃에서 6∼9일간 싹을 내어 싹의 길이가 곡립의 1∼1.5배 정도가 되었을 때 발아를 중지시키고 50∼65℃의 온도에서 약 20∼24시간 건조한 후 정선, 분쇄하여 얻은 발아벼 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 11.6%의 발아벼발효주 2,590㎖를 제조하였다 Example 1: The raw rice was immersed in water at about 15 ° C for 48 to 72 hours to sufficiently absorb the moisture necessary for germination, and then sprouted at 16 to 22 ° C for 6 to 9 days, the shoot length being 1 to 1.5 times the grain. After the germination was stopped, the germination was stopped and dried at a temperature of 50 to 65 ° C. for about 20 to 24 hours, followed by 1,000 g of germinated rice obtained by selection and grinding. -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented 4 to 5 days at 2,590ml of germinated rice fermented wine with alcohol content of 11.6% was prepared.

비교예 2: 생옥수수를 분쇄한 분쇄옥수수 1,000g에 급수 2,500㎖ 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 9.8%의 옥수수발효주 2,200㎖를 제조하였다 Comparative Example 2 1,000 g of crushed corn crushed raw corn to 2,500 ml liquefied enzyme -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented for 4 to 5 days at 2,200ml of corn fermented liquor with alcohol content of 9.8% was prepared.

실시예 2 :생옥수수를 약 15℃내외의 물에 48∼72시간 침지하여 발아에 필요한 수분을 충분히 흡수시킨 후 16∼22℃에서 6∼9일간 싹을 내어 싹의 길이가 곡립의 1∼1.5배 정도가 되었을 때 발아를 중지시키고 50∼65℃의 온도에서 약 20∼24시간 건조한 후 정선, 분쇄하여 얻은 발아옥수수 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 11.0%의 발아옥수수발효주 2,640㎖를 제조하였다 Example 2 Fresh corn was immersed in water at about 15 ° C. for 48 to 72 hours to sufficiently absorb the necessary water for germination, and then sprouted at 16 to 22 ° C. for 6 to 9 days, the length of shoots being 1 to 1.5 When the embryos were fertilized, germination was stopped, dried at about 50-65 ° C. for about 20 to 24 hours, and then 1,000 g of germinated corn obtained by selection and grinding were fed with 2,500 ml of water and liquefied enzyme ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented 4 to 5 days at 2,640ml of germinated corn fermented alcohol with 11.0% alcohol content was prepared.

비교예 3: 생밀을 분쇄한 분쇄밀 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 10.2%의 밀발효주 2,312㎖를 제조하였다 Comparative Example 3 : 1,000 g of crushed wheat flour, water 2,500 ml and liquefied enzyme ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. 2,312 ml of wheat fermented liquor with alcohol content of 10.2% were prepared by fermentation for 4 to 5 days.

실시예 3 :생밀을 약 15℃내외의 물에 48∼72시간 침지하여 발아에 필요한 수분을 충분히 흡수시킨 후 16∼22℃에서 6∼9일간 싹을 내어 싹의 길이가 곡립의 1∼1.5배 정도가 되었을 때 발아를 중지시키고 50∼65℃의 온도에서 약 20∼24시간 건조한 후 정선, 분쇄하여 얻은 발아밀 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 12.2%의 발아밀발효주 2,795㎖를 제조하였다 Example 3: The raw wheat was immersed in water at about 15 ° C. for 48 to 72 hours to sufficiently absorb the moisture necessary for germination, and then sprouted at 16 to 22 ° C. for 6 to 9 days, the shoot length being 1 to 1.5 times the grain. When the germination was reached, the germination was stopped, dried at a temperature of 50 to 65 ° C. for about 20 to 24 hours, and then 1,000 g of germinated wheat obtained by selection and grinding were fed with 2,500 ml of water and liquefied enzyme ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented 4 to 5 days at 2,795ml of germinated wheat wine with 12.2% alcohol content was prepared.

비교예 4: 생벼를 분쇄한 분쇄벼 500g과 생옥수수를 분쇄한 분쇄옥수수 500g을 혼용한 분쇄곡류 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 9.3%의 벼,옥수수혼용발효주 1,975㎖를 제조하였다 Comparative Example 4 1,000 g of crushed grains mixed with 500 g of crushed rice and 500 g of crushed corn, mixed with 500 g of water, and liquefied enzyme ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented for 4 to 5 days at 1,975ml of rice, corn mixed fermented liquor with alcohol content of 9.3% was prepared.

실시예 4 :벼, 옥수수를 약 15℃내외의 물에 48∼72시간 침지하여 발아에 필요한 수분을 충분히 흡수시킨 후 16∼22℃에서 6∼9일간 싹을 내어 싹의 길이가 곡립의 1∼1.5배 정도가 되었을 때 발아를 중지시키고 50∼65℃의 온도에서 약 20∼24시간 건조한 후 정선, 분쇄하여 얻은 발아벼 500g과 발아옥수수 500g을 혼용한 발아곡류 1,000g에 급수 2,500㎖ 및 액화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고, 95℃의 온도에서 약 30분간 액화(糊化)시킨 후 액화액을 65℃로 냉각시킨 다음, 당화효소제(-Amylase, 역가 : 10,000units/g) 20units/g을 첨가하고 65℃의 온도에서 약 2시간 당화시켜 당화액을 완성한 다음, 완성된 당화액에 Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 알코올함량 11.2%의 발아벼,발아옥수수혼용발효주 2,615㎖을 제조하였다 Example 4 After soaking rice and corn in water at about 15 ° C. for 48 to 72 hours to sufficiently absorb the necessary moisture for germination, shoots were produced at 16 to 22 ° C. for 6 to 9 days, and the length of the shoots was 1 to When it became 1.5 times, the germination was stopped and dried for about 20 to 24 hours at a temperature of 50 to 65 ° C., and 500 g of germinated rice and 500 g of germinated corn obtained by selection and grinding were mixed with 1,000 g of germinated grains to supply 2,500 ml of water and liquefied enzyme. ( -Amylase, Activity: 10,000units / g) 20units / g is added, and the solution is liquefied at a temperature of 95 ° C for about 30 minutes, and then the liquid is cooled to 65 ° C. Amylase, Activity: 10,000units / g) Add 20units / g and saccharify at about 65 ℃ for 2 hours to complete the saccharified solution, and then add Saccharomyces koreanus yeast culture solution to the completed saccharified solution at a temperature of 23 ~ 25 ℃. Fermented 4 to 5 days at 2,615 ml of germinated rice, germinated corn mixed fermented alcohol with 11.2% alcohol content was prepared.

[표1]Table 1

담금배합표(발아, 액화, 당화, 발효공정)Dipping table (germination, liquefaction, saccharification, fermentation process)

* sp(Saccharogenic Power) : 발아곡류 또는 누룩 1g이 가용성전분 1g에 작용하여 생성된 포도당을 가용성전분 1g에 대하여 백분율로 나타낸 수치* sp (Saccharogenic Power): The amount of glucose produced by 1g germinated grain or yeast acting on 1g of soluble starch as a percentage of 1g of soluble starch

각 원료의 전분가는 벼, 옥수수, 밀이 각각 60%, 66%, 70% 발아벼, 발아옥수수, 발아밀이 각각 55%, 60%, 64%로 분석되었으며, 이는 발아시 일부전분이효소의 영양원으로 공급된데 기인한 것으로 판단되었다.Starch value of each raw material was 60%, 66%, 70% germinated rice, germinated corn and germinated wheat, respectively 55%, 60% and 64%, respectively. It was believed to be due to the supply of nutrients.

각 원료의 엑기스는 전분을 포함한 모든 가용성(可溶性)성분의 총합계를 나타내는 것으로서 벼, 옥수수, 밀은 각각 77%, 82%, 85%를, 발아벼, 발아옥수수, 발아밀이 각각 71%, 73%, 75%로 분석되어 발아시 전분소실비율은 약6%∼9%내외로 분석되었다.The extract of each raw material represents the total of all soluble components including starch. Rice, corn and wheat were 77%, 82% and 85%, respectively. Germinated rice, germinated corn and germinated wheat were 71% and 73, respectively. % And 75%, the starch loss rate during germination was about 6% to 9%.

각 원료의 당화력(Diastatic Power)은 발아벼, 발아옥수수가 각각 43sp, 52sp정도로 분석되어 발아벼, 발아옥수수는 기존 맥아(약 250sp)의 당화력에 비해낮게 나타났으나, 발아밀은 228sp로 기존 맥아와 비슷한 수준으로 나타나 발아시 효소가 충분히 생성되었다는 것과 별도의 효소제 첨가없이도 액화 및 당화가 가능하다는 것을 알 수 있었다.Germinated rice and germinated corn were analyzed as 43sp and 52sp, respectively. The germinated rice and germinated corn were lower than those of conventional malt (about 250sp), but germinated wheat was 228sp. At the level of, the enzyme was sufficiently produced during germination and liquefaction and saccharification were possible without additional enzyme.

액화효소제(-Amylase) 및 당화효소제(-Amylase)의 사용량은 역가 10,000units/g 기준 각각 20units/g을 사용하였다.Liquefied enzyme Amylase) and glycosylase ( -Amylase) was used 20units / g each based on the titer 10,000 units / g.

[표2][Table 2]

발아, 호화 및 당화, 발효공정 종료후 각종비율 산정Calculation of various ratios after germination, gelatinization and saccharification, and fermentation process

* 발아비율(%) = [발아후 곡류갯수/발아전 곡류갯수]×100 (무작위 추출법)* Germination rate (%) = [number of grains after germination / number of grains before germination] × 100 (random sampling)

* 급수비율(%) = [급수량(㎖)/원료곡류사용량(g)]×100* Water supply rate (%) = [water supply (ml) / raw grain consumption (g)] × 100

* 당화비율(%) = [{당화액량(㎖)x당화액Brix度/{원료사용량(g)x원료의엑기스함량(%)}]×100* Saccharification ratio (%) = [{Saccharification liquid (ml) x saccharification liquid Brix 度 / {raw material usage (g) x extract content (%)}]] x 100

* 술덧숙성비율(%) = [숙성술덧량(㎖)/당화즙사용량(㎖)]×100* Maturation ratio (%) = [Maturation liquor amount (ml) / Saccharification juice usage (ml)] × 100

* 대원료주박비율(%) = [주박량(㎖)/원료사용량(g)]×100* Raw Material Slurry Ratio (%) = [Pleated Shrinkage (ml) / Raw Material Usage (g)] × 100

* 발효비율(%) = [{숙성술덧량(㎖)x숙성술덧알코올분(%)}/{원료사용량(g)×원료전분가(%)x0.715}]×100* Fermentation Ratio (%) = [{Maturation Sulfonate (ml) x Aged Sulfur Alcohol (%)} / {Raw Material Consumption (g) x Raw Material Starch (%) x0.715}] × 100

* 대원료제성비율(%) = 시험구별 상대비교를 하기 위해 알코올분 10%규격에 맞추어 산정* Raw material formulation ratio (%) = Calculated according to the alcohol content 10% for comparative comparison by test type

= [알코올분 10%로 환산한 제성수량(㎖)/원료사용량(g)]×100= [Formulation quantity (ml) / raw material use (g) converted into alcohol content 10%] x 100

발아비율은 발아전 원료곡류 갯수대비 발아후 발아완료된(미발아곡류 제외)곡류의 백분율로서 일정갯수를 무작위 추출하여 산출하였으며, 품질이 비교적 우수한 종자용 곡류를 사용하여 거의 100%에 가까운 발아율을 나타내었다.The germination ratio was calculated by randomly extracting a certain number as the percentage of grains after germination (excluding ungerminated grains) to the number of raw grains before germination, and showed nearly 100% germination rate by using seed grains of relatively high quality. It was.

급수비율은 각 시험구 공히 250%로서 원료곡류 1,000g당 2,500㎖의 급수를 첨가하여 호화 및 당화를 진행시켰다. 이는 약 92%의 당화율을 적용시 24Brix 정도의 엑기스를 함유할 수 있는 계산에 의한 이론적 급수비율을 참고하였으며, 실제시험결과에서 나타난 각 시험구의 Brix度인 20∼23과 비교시 큰 차이는 없었다.The feed rate was 250% in each test zone, and 2,500 ml of feed water was added per 1,000 g of raw grains to proceed with gelatinization and saccharification. This is based on the theoretical water supply ratio calculated by the calculation that can contain about 24 Brix extract when applying about 92% glycosylation rate, and there is no big difference when compared with 20 ~ 23 Brix degree of each test in the actual test results. .

당화비율은 비교예1, 2, 3, 4시험구가 각각 84%, 74%, 87%, 79%로 나타났고, 실시예1, 2, 3, 4시험구는 96% 97%, 98%, 96%로 나타나 각 시험구별 동일한 조건에서 호화 및 당화 시켰음에도 불구하고(오히려 발아벼, 발아옥수수 시험구는 액화 및 당화효소제를 비교예시험구보다 약 2/3으로 감하여 첨가) 벼, 옥수수를 원료로 한 비교예시험구보다 발아벼, 발아옥수수를 원료로 한 실시예시험구가 10%이상 높게 나타났다. 이는 곡류발아시 자체효소생성과정에서의 원료자체의 물성변화로 호화 및 당화조작이 훨씬 용이하였을 것으로 판단되었다.The saccharification ratio was 84%, 74%, 87%, and 79% in Comparative Examples 1, 2, 3, and 4, respectively, and 96% 97%, 98%, in Examples 1, 2, 3, and 4, respectively. Although 96% showed gelatinization and saccharification under the same conditions for each test group (rather than germinated rice and germinated maize test plots, liquefaction and glycation enzymes were reduced by about 2/3 of the comparative test plots), rice and corn were used as raw materials. It was found that the Example test plots made from germinated corn and germinated corn were more than 10% higher than the comparative sample test plots. It was judged that gelatinization and saccharification were much easier due to the change of physical properties of the raw material itself during grain germination.

발효비율은 비교예1, 2, 3, 4시험구가 각각 66.9%, 64.6%, 64.0%, 63.1%로 나타났고, 실시예1, 2, 3, 4시험구는 89.1% 76.9%, 83.0%, 81.5%로 나타나 각 시험구별 동일한 조건에서 발효를 시켰음에도 불구하고 벼, 옥수수, 밀을 원료로 한 비교예시험구보다 발아벼, 발아옥수수, 발아밀을 원료로 한 실시예시험구가 20%이상 높게 나타났다. 이는 호화 및 당화시 당화율의 차이만큼 발효과정에서도 차이가 나타난 것으로 확인되었다.The fermentation ratio was 66.9%, 64.6%, 64.0%, 63.1% in Comparative Examples 1, 2, 3, and 4, respectively, and 89.1%, 76.9%, 83.0% in Examples 1, 2, 3, and 4, respectively. Although 81.5% showed fermentation under the same conditions for each test group, 20% or more of the test groups made from germinated rice, germinated corn, and germinated wheat were higher than the comparative test made of rice, corn, and wheat. appear. It was confirmed that the difference in the fermentation process as much as the difference in the saccharification rate during gelatinization and saccharification.

대원료제성비율은 비교예1, 2, 3, 4시험구가 각각 202%, 190%, 235%, 184%로 나타났고, 실시예1, 2, 3, 4시험구는 300% 290%, 340%, 293%로 나타나 벼, 옥수수, 밀을 원료로 한 비교예시험구보다 발아벼, 발아옥수수, 발아밀을 원료로 한 실시예시험구가 90%이상 높게 나타났다. 이는 호화 및 당화시 당화율의 차이만큼 발효비율에서도 차이가 있었고, 결과적으로 생산량면에서 동일한 호화ㆍ당화ㆍ발효조건에서는 미발아곡류보다 발아곡류를 원료로한 시험구가 유리하다는 것을 알 수 있었다.The raw material formulation ratios were 202%, 190%, 235%, and 184% in the test samples of Comparative Examples 1, 2, 3, and 4, respectively, and 300%, 290%, and 340 of Test Examples 1, 2, 3, and 4, respectively. %, 293%, showed more than 90% higher than the experimental example of the rice, corn, wheat wheat germinated rice, germinated corn, germinated wheat. The difference in fermentation ratio was as much as the difference in the degree of saccharification at the time of gelatinization and saccharification, and as a result, it was found that the test plots made from germinated grains were more advantageous than ungerminated grains under the same gelatinization, saccharification and fermentation conditions.

[표3]Table 3

제성주의 일반분석Sacralism General Analysis

주정분은 비교예1, 2, 3, 4시험구가 각각 9.2%, 9.8%, 10.2%, 9.3%를 나타냈고, 실시예1, 2, 3, 4시험구가 각각 11.6%, 11.1%, 12.2%, 11.2%를 나타내 동일한 조건에서 호화ㆍ당화ㆍ발효시켰음에도 불구하고 약 2%정도의 차이를 나타냈다. 이는 각종비율산정에서도 설명된 바와 같이 당화비율, 발효비율의 차이에 기인하는 것으로서 결과적으로 수율(알코올수득량)면에서 동일한 호화ㆍ당화ㆍ발효조건에서는 미발아곡류보다 발아곡류를 원료로한 시험구가 월등히 앞선다는 것을 확인할 수 있었다.As for the main portion, Comparative Examples 1, 2, 3 and 4 showed 9.2%, 9.8%, 10.2% and 9.3%, respectively. Examples 1, 2, 3 and 4 showed 11.6%, 11.1% and 12.2, respectively. % And 11.2% were shown, the difference was about 2% even though gelatinization, saccharification and fermentation were performed under the same conditions. This is due to the difference in the saccharification ratio and the fermentation ratio, as explained in the various ratio calculations, and as a result, a test piece made from germinated grains rather than ungerminated grains under the same gelatinization, saccharification and fermentation conditions in terms of yield (alcohol yield). We can see that it is far ahead.

산도는 각 시험구별 차이가 없었으나 아미노산도는 비교예1, 2, 3, 4시험구가 각각 0.3, 0.4, 0.6, 0.2를 나타냈고, 실시예1, 2, 3, 4시험구가 각각 0.7, 1.1, 1.4, 0.9를 나타내 동일한 조건에서 호화ㆍ당화ㆍ발효시켰음에도 불구하고 약 2배에서 4배정도의 차이를 나타냈다. 이는 곡류원료의 발아과정에서 생성되는 단백질 분해효소에 의해서 아미노산이 더 많이 생성되는 것에 기인하는 것으로 판단되었다.The acidity was not different for each test group, but the amino acid level was 0.3, 0.4, 0.6, 0.2 for Comparative Examples 1, 2, 3, and 4, respectively, and 0.7 for Test Example 1, 2, 3, and 4, respectively. , 1.1, 1.4, and 0.9, which showed the difference of about 2 to 4 times despite the gelatinization, saccharification, and fermentation under the same conditions. This was due to the production of more amino acids by the proteolytic enzymes produced during the germination of cereals.

[표4]Table 4

제성주의 향기성분 함량(GC분석)Flavor Compound Contents of Jeju Wine (GC Analysis)

주1) 노르말프로필알코올, 이소부틸알코올, 이소아밀알코올 등을 총칭하며, 아미노산발효에 의해 생성되는 주류 향기성분의 일종으로 자극취가 강함.Note 1) Generic propyl alcohol, isobutyl alcohol, isoamyl alcohol, etc. are generically named. It is a kind of mainstream scent component produced by amino acid fermentation and has a strong irritant smell.

* 분석조건 :* Condition of Analysis:

- G.C : M600D-G.C: M600D

- Detector FID-Detector FID

- Injection temp : 170℃-Injection temp: 170 ℃

- Detector temp : 220℃-Detector temp: 220 ℃

- Carrier gas : N2 Carrier gas: N 2

상기 [표4]에서 보는 바와 같이 주류에 자극적인 향을 부여하는 퓨젤유는 미발아곡류를 원료로 당화ㆍ발효시킨 비교예1, 2, 3, 4시험구에서 각각 826ppm, 965ppm, 578ppm, 932ppm을, 발아곡류를 원료로 당화ㆍ발효시킨 실시예1, 2, 3, 4시험구에서는 각각 1,102ppm, 1,258ppm, 1,002ppm, 1,018ppm을 나타내 차이는 미미하나 일반곡류원료보다는 발아곡류를 원료로 당화ㆍ발효시킬 경우 향기면에서 다소 독특한 특징을 부여할 수 있는 것으로 파악되었다.As shown in [Table 4], the fusel oil which gives a stimulating aroma to the liquor was 826ppm, 965ppm, 578ppm, and 932ppm in Comparative Examples 1, 2, 3, and 4, respectively, in which unfermented grains were saccharified and fermented. , 1, 2, 3, and 4 fermented saccharified and fermented raw materials showed 1,102ppm, 1,258ppm, 1,002ppm and 1,018ppm, respectively. Fermentation was found to be able to impart some unique characteristics in terms of fragrance.

[표5]Table 5

제성주의 향미성분 함량(LC분석)Flavor Compound Content of Jeju Wine (LC Analysis)

* 분석조건 :* Condition of Analysis:

- Detector : UV(210nm)-Detector: UV (210nm)

- Column : Supelcogel C-610H, 30cm×7.8mmID-Column: Supelcogel C-610H, 30cm × 7.8mmID

- Mobile phase: 0.1% Phosphoric acidMobile phase: 0.1% Phosphoric acid

- Injection volume : 5㎕-Injection volume: 5µl

유기산 분석결과 총 유기산 합계치가 비교예1의 벼시험구에서는 1,275ppm, 실시예1의 발아벼시험구에서는 689ppm이 검출되어 발아벼시험구에서 낮게 검출된 반면, 비교예2의 옥수수시험구에서는 633ppm, 실시예2의 발아옥수수시험구에서는 3,648ppm, 비교예3의 밀시험구에서는 754ppm, 실시예3의 발아밀시험구에서는 785ppm이 검출되어 발아벼보다는 발아옥수수, 발아밀에서 비교적 많은 유기산이 검출됨을 알 수 있었으며, 벼,옥수수혼용시험구인 비교예4 시험구에서는 1,851ppm, 발아벼,발아옥수수혼용시험구인 실시예4 시험구에서는 1,637ppm이 검출되어 역시 벼, 발아벼단용시험구보다는 많은 유기산이 검출되어 유기산에 의한 향미면에서는 발아옥수수, 발아밀이 앞선 것으로 파악되었다.As a result of the analysis of the organic acid, the total organic acid was 1,275ppm in the rice test plot of Comparative Example 1 and 689ppm in the germination rice test sample of Example 1, which was low in the germination rice test plot, whereas 633ppm in the corn test plot of Comparative Example 2 , 3,648ppm in the germinated corn test zone of Example 2, 754ppm in the wheat test zone of Comparative Example 3, 785ppm in the germinated wheat test zone of Example 3 was detected, and relatively more organic acids were detected in germinated corn and germinated wheat than germinated rice. 1,851ppm was detected in the Comparative Example 4 test plot, which is a mixed test of rice and corn, and 1,637ppm was detected in the Test Example 4 which was the mixed test of germinated rice and germinated corn, and more organic acids than the rice and germinated rice test were also found. It was detected that germinated corn and germinated wheat were superior in flavoring with organic acids.

[표6]Table 6

제성주의 아미노산성분 함량(아미노산분석기)Amino Acid Content of Detergent Wine (Amino Acid Analyzer)

* 분석조건 :* Condition of Analysis:

- 분석기기 : L-8500 아미노산분석기Analyzer: L-8500 Amino Acid Analyzer

- Column : Ion-Exchange ColumnColumn: Ion-Exchange Column

- Mobile phase: 완충용액 pH1∼pH6, 닌히드린용액-Mobile phase: buffer solution pH1∼pH6, ninhydrin solution

- Injection volume : 20㎕Injection volume: 20µl

- standard : Amino Acid 混合標準液(18종 아미노산)standard: Amino Acid (18 amino acids)

아미노산 분석결과 총 아미노산 합계치가 비교예1 벼시험구, 비교예2 옥수수시험구, 비교예3 밀시험구, 비교예4 벼,옥수수혼용시험구에서는 각각 15ppm, 16ppm, 25ppm, 13ppm이 검출된 반면, 실시예1 발아벼시험구, 실시예2 발아옥수수시험구, 실시예3 발아밀시험구, 실시예4 발아벼,발아옥수수혼용시험구에서는 각각 36ppm, 87ppm, 98ppm, 56ppm이 검출되어 동일한 조건에서 호화ㆍ당화ㆍ발효시켰음에도 불구하고 약 2배에서 4배정도의 차이를 나타냈다.As a result of the amino acid analysis, the total amino acid value of 15 ppm, 16 ppm, 25 ppm, and 13 ppm were detected in Comparative Example 1 rice test, Comparative Example 2 corn test, Comparative Example 3 wheat test, Comparative Example 4 rice and corn mixed test, respectively. , Example 1 germinated rice test, Example 2 germinated corn test, Example 3 germinated wheat test, Example 4 germinated rice, germinated corn mixed test was detected 36ppm, 87ppm, 98ppm, 56ppm respectively In spite of the gelatinization, saccharification and fermentation, the difference was about 2 to 4 times.

이는 앞서 설명된 바와 같이 곡류원료의 발아과정에서 단백질 분해효소에 의해 단백질이 아미노산으로 분해된 것에 기인하는 것으로 판단되었다.As described above, it was determined that the protein was decomposed into amino acids by proteolytic enzymes during the germination of grain raw materials.

제성주의 관능평가는 물리·화학적인 분석데이터(GC, LC, Amino산분석기를 이용한 기기분석결과)를 참고하여 실온 20℃전후, 상대습도 60%로 공기조절되는 관능검사실에서 주류분석업무 등을 10년이상 해온 전문패널 5명이 제조된 시험구별 시료에 대하여 향미 등의 특징을 상세히 평가하는 "기술법" (패널요원의 의견을 종합하여 서술식으로 평가하는 방법)을 채택하여 3회이상 실시하였다.첫째, 색택에 있어 비교예1 벼시험구는 미색(쌀뜨물), 비교예2 옥수수시험구는 베지색, 비교예3 밀시험구는 진한미색을 나타냈고, 비교예4 벼, 옥수수혼용시험구는 미색과 베지색의 중간을 나타냈으며, 실시예1 발아벼시험구는 베지색, 실시예2 발아옥수수시험구는 옅은진흙색, 실시예3 발아밀시험구는 옅은갈색을 나타냈고, 실시예4 발아벼, 발아옥수수혼용시험구는 베지색과 옅은진흙색의 중간을 나타내 발아곡류를 원료로 한 실시예가 비교예에 비해 비교적 짙은 색상을 나타냈다.For the sensory evaluation of the sexualism, refer to the physical and chemical analysis data (GC, LC, and Amino acid analyzer results), and perform liquor analysis in the sensory laboratory, which is air-conditioned at 20% and 60% relative humidity. Five professional panelists who have been in the field for more than 10 years have adopted the "Technology Act" (the method of evaluating the opinions of panelists in a descriptive manner) that evaluates the characteristics of flavors in detail. In color selection, Comparative Example 1 rice test plot was off-white (rice water), Comparative Example 2 corn test strip was beige, and Comparative Example 3 wheat test strip was dark white, and Comparative Example 4 rice and corn mixed test were off-white and beige. In Example 1, the germination of the germination rice field was beige, the germination of the germination corn of Example 2 was light mud, and the germination of the germination mill of the Example 3 was light brown. Rolling Bede One embodiment the germinated grains are shown in the middle of a color and a pale color to the clay raw material examples exhibited relatively dark color compared with the comparative example.

둘째, 향기에 있어 비교예1 벼시험구는 쌀을 갈아 물에 용해하였을 경우 미미하게 느껴지는 고소한 향을, 비교예2 옥수수시험구는 기존 옥수수를 증자하였을 때의 향을, 비교예3 밀시험구는 볏짚을 물에 삶을 때의 은은한 향을 나타냈고 비교예4 벼, 옥수수혼용시험구는 비교예1, 2의 중간을 나타낸 반면, 실시예1 발아벼시험구는 발아 후 65℃의 오븐에서 1일정도 건조시 풍기는 발아벼 특유의 고소한 향을 그대로 반영하였고, 실시예2 발아옥수수 및 실시예3의 발아밀시험구도 발아ㆍ건조시 풍기는 발아옥수수 또는 발아밀 특유의 고소한 향을 그대로 반영(향은 각각 다르나 발아벼보다 세기에 있어서 더욱 진하게 느껴짐)하였으며, 실시예4 발아벼, 옥수수혼용시험구는 실시예1, 2의 중간을 나타내어 적절히 혼화된 양쪽의 맛을 느낄수 있어 미발아곡류보다는 발아곡류를 원료로 한 시험구에서 발아, 건조시 풍기는 고소한 향기를 진하게 느낄수 있었다.Secondly, in the fragrance, the comparative example 1 rice test sphere was a savory scent that was felt insignificantly when the rice was ground and dissolved in water, the comparative example 2 corn test zone was the fragrance when the corn was added, and the comparative example 3 wheat test sphere was rice straw. When boiled in water, it showed a mild fragrance, and Comparative Example 4 rice and corn mixed test plots showed the middle of Comparative Examples 1 and 2, while Example 1 germinated rice plots were dried in an oven at 65 ° C. for 1 day after germination. The air swell reflects the savory aroma unique to germinated rice, and Example 2 germinated corn and the germinated wheat test spheres of Example 3 reflect the scents unique to germinated corn or germinated wheat during germination and drying. More intense in the intensity), Example 4 germinated rice, corn mixed test spheres are shown in the middle of Examples 1, 2 can feel the taste of both properly mixed Could feel the thick aroma drifting during germination and drying in a germination test nine grains as a raw material.

셋째, 맛에 있어서도 원료각각의 특징있는 맛이 그대로 주질에 전가되었다. 비교예1 벼시험구는 쌀을 갈아 물에 용해하였을 경우 미미하게 느껴지는 고소한 맛에 약간의 알코올취를 느낄 수 있었고, 비교예2 옥수수시험구 및 비교예3의 밀시험구는 기존 옥수수 또는 밀을 증자하였을 때의 맛에 약간의 알코올취를 느낄수 있었으며, 비교예4 벼, 옥수수혼용시험구는 비교예1, 2의 중간을 나타낸 반면, 실시예1 발아벼시험구는 발아 후 65℃의 오븐에서 1일정도 건조시 풍기는 발아벼 특유의 고소한 향이 그대로 맛에 반영되어 고소한 맛이 비교예에 비해 배가되었고, 실시예2 발아옥수수 및 실시예3 발아밀시험구도 발아ㆍ건조시 풍기는 발아옥수수 또는 발아밀 특유의 고소한 맛(미숫가루의 맛과 비슷)을 그대로 반영(맛은 각각 다르나 발아벼보다 세기에 있어서 더욱 진하게 느껴짐)하였다. 실시예4 벼, 옥수수혼용시험구는 실시예1, 2의 중간을 나타내어 적절히 혼화된 양쪽의 맛을 느낄 수 있었다.Third, in terms of taste, the characteristic taste of each raw material was transferred to the main as it is. Comparative Example 1 The rice test was able to feel a slight alcohol odor with a slight taste when the rice was dissolved in water, and Comparative Example 2 corn test and wheat mill of Comparative Example 3 would increase the existing corn or wheat. A little alcohol odor was felt in the taste, while Comparative Example 4 rice and corn mixed test plots showed the middle of Comparative Examples 1 and 2, while Example 1 germinated rice test pads were dried in an oven at 65 ° C. for 1 day after germination. The savory flavor peculiar to germinated rice was reflected in the taste as it was, and the savory taste doubled compared to the comparative example. Example 2 Germinated corn and Example 3 Germinated wheat germ, the germinated corn during the germination and drying (Similar to the taste of brown rice flour), it is reflected as it is (taste is different but feels darker in strength than germinated rice). Example 4 The rice and corn mixed test spheres showed the middle of Examples 1 and 2, and were able to feel the taste of both properly mixed.

색택, 향, 맛을 종합하면, 발아시키지 않은 미발아 곡류를 원료로 한 비교예시험구보다 발아곡류를 원료로한 실시예시험구에서 발아ㆍ건조시 생성된 비타민, 미네랄, 아미노산 등 각종 영양원과 풍부하게 느껴지는 곡류특유의 고소한 향미와 향기성분이 그대로 전가되었고 또한 액화, 당화, 자비, 발효공정으로 제조된 단행복발효식 발효주로서 액화 및 당화효소제만 사용하므로서 입국 또는 누룩에서 유래되는 구연산, 젖산, 곰팡이취가 전혀 느껴지지 않아 기존탁주와는 전혀 다른 외관이 맑으며, 담백ㆍ고소하고 깔끔한 주질을 나타내었음을 유기산 및 아미노산 기기분석결과를 통해 확인할 수 있었다.By combining color, aroma and taste, various nutritional sources such as vitamins, minerals and amino acids produced during germination and drying in Example Test Zones using germinated grains than Comparative Test Tests using non-germinated grains as raw materials It is a simple fermented fermented liquor produced by liquefaction, saccharification, mercy, fermentation process, and only citric acid, lactic acid, mold derived from yeast or yeast by using only liquefaction and glycosylating enzyme. The organic acid and amino acid device analysis results showed that the odor was not felt at all and the appearance was completely different from the existing Takju, and it showed a light, pure and clean quality.

본 발명의 방법에 의해 제조된 발아곡류발효주는 벼, 옥수수, 밀을 발아, 건조시킨 발아곡류를 원료로 하여 입국, 누룩 등 발효제를 사용하지 않고 액화 및 당화효소제만으로 당화, 발효시키는 단행복발효방식에 의해 만들어진 발효주로써 기존의 입국, 누룩 등 곡류를 원료로 한 발효주에서 유래되는 곰팡이취와 산취를 배제하고 아울러 발아시 생성되는 곡류효소와 비타민, 미네랄, 각종 아미노산류 등 풍부한 영양소와 발아후 건조시 생성되는 곡류특유의 향미, 향기성분을 제성주에 그대로 부가시키는 새로운 형태의 주류제조방법에 관한 것으로써 발효주 시장의 활성화와 더불어 남아도는 쌀등 곡류소비촉진에도 크게 기여할 수 있는 효과가 있다.The germinated grain fermented wine prepared by the method of the present invention is a simple fermentation method in which the germinated grains are germinated with rice, corn, and wheat as raw materials, and are glycosylated and fermented with only liquefied and glycated enzymes without using fermentation agents such as entry and leaven. It is a fermented liquor made by fermented liquor, which excludes mold odor and acid odor derived from fermented liquor made from grains such as conventional yeast and yeast, and is rich in nutrients such as grain enzymes, vitamins, minerals and various amino acids produced during germination and drying after germination. It relates to a new type of liquor manufacturing method that adds the flavor and aroma component of grains to sake liquor as it is, which can greatly contribute to the promotion of fermented liquor market and the consumption of grains such as rice.

Claims (4)

생벼를 발아(發芽)시키고 건조시킨 다음 분쇄한 발아벼에 발아곡류원료의 250%에 해당하는 급수 및-Amylase(역가 : approx. 10,000units/g) 20units/g을 첨가한 후 95℃의 온도에서 30분간 액화(糊化)시키고, 그 액화액을 65℃로 냉각시킨 다음-Amylase(역가 : approx. 10,000units/g) 20units/g을 첨가하여 65℃의 온도에서 2시간 당화(糖化)시키고, 그 당화액을 23℃로 냉각시킨 다음, Saccharomyces koreanus효모배양액을 첨가하여 23∼25℃의 온도에서 4∼5일간 발효시켜 단행복발효법에 의한 알코올분 약 10%내외의 발효주를 얻는 것을 특징으로 하는 발아벼를 이용한 발효주의 제조방법Raw water is germinated, dried, and then crushed germinated rice to supply 250% of the germinated cereal raw material and After adding 20 units / g of Amylase (titer: approx. 10,000 units / g), the solution is liquefied at a temperature of 95 ° C. for 30 minutes, and the liquid is cooled to 65 ° C. -Amylase (titer: approx. 10,000 units / g) 20 units / g was added to saccharify at 65 ° C. for 2 hours, the saccharified solution was cooled to 23 ° C., and then Saccharomyces koreanus yeast culture solution was added. Fermented liquor using germinated rice, characterized in that fermented liquor of about 10% of alcohol content by single fermentation method by fermentation at temperature of -25 ° C for 4-5 days. 삭제delete 삭제delete 삭제delete
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KR20170005638A (en) 2015-07-06 2017-01-16 한영석 Germination yeast and method manufacturing thereof
KR102246997B1 (en) 2019-12-10 2021-04-30 배병태 Restaurant Table

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KR100868577B1 (en) * 2008-03-20 2008-11-14 대한민국 Method for preparing fermented rice wine using unhulled rice and rice
CN104745390A (en) * 2015-04-01 2015-07-01 谢传桂 Method for making wine by rice embryo buds

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170005638A (en) 2015-07-06 2017-01-16 한영석 Germination yeast and method manufacturing thereof
KR102246997B1 (en) 2019-12-10 2021-04-30 배병태 Restaurant Table

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