KR100408692B1 - Precise suction and delivering device for glassware pipette - Google Patents

Abstract

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a precision suction and discharge device for a glass pipette, in which a pipette is connected to a mechanism for generating a vacuum pressure, and the reagent is sucked, and the reagents are removed from the pipette in a large amount or a small amount while removing the vacuum pressure with various control valves. It is configured to be discharged by a plurality of pipettes, especially by mounting a plurality of pipettes at a time or a pipette having a different diameter used in the vacuum generating mechanism, by using a plurality of pipettes at the same time or different reagents at the same time It is an object to provide a precision suction and discharge device for glass pipettes that is capable of inhaling / draining as well as allowing a more accurate and consistent discharge of the amount of reagent through each control valve.

The present invention for realizing this object is to hold and fix the reagent suction / discharge mechanism for generating a vacuum pressure to suck the reagent and to discharge the reagent by releasing the vacuum pressure, and the pipette connected to the reagent suction / discharge mechanism It is characterized by consisting of a pipette fixing forceps, and a vertical transfer mechanism for supporting the pipette fixing forceps to adjust the height of the pipette.

Description

Precise suction and delivering device for glassware pipette

The present invention relates to a precision suction and discharge device for a glass pipette, and more particularly, to generate a vacuum pressure to suck the reagent into the pipette, and to discharge the reagent from the pipette while removing the vacuum pressure with various valves. The present invention relates to a precision pipe suction and discharge device of a glass pipette, which is convenient and safe to use, and which can be used to more precisely control the amount of reagent.

In general, a laboratory is equipped with a large number of experimental equipment, and the equipment suitable for various experiments is selected and used according to the purpose of the experiment.

Among them, pipettes are used a lot as experimental materials used to remove or add a certain amount of liquid reagents to other solutions or to remove them. The material is usually made of glass, but it may be made of plastic depending on the purpose. .

Such a pipette is most commonly used as a one-pipe pipette 100a made of a thin glass tube having a predetermined diameter and having a convex portion in a middle length as shown in FIG. 4A.

The hole pipette 100a dips the lower end of the pipette into a beaker or reagent container containing the reagent, sucks the upper end of the pipette into the mouth, generates a vacuum pressure inside the pipette, and inhales the reagent. While removing your mouth from the top of the pipe, you can cover the top of the pipette with your hands, transfer the pipette to another laboratory container that will hold the reagents, remove your hand from the top of the pipette, remove the vacuum and relieve the reagent.

However, the hole pipette 100a is harmful to the tester when the reagent has a harmful gas or odor because the hole pipette 100a needs to bite the upper part of the pipette into the mouth and directly suck the air inside the pipette to generate a vacuum pressure. As a result, the problem of being restricted from use has arisen. In addition, if the reagent is sucked too strongly in the process of inhaling the reagent, the reagent may enter the mouth and cause a safety accident. On the contrary, if too weakly sucked, the reagent may not be sucked as much as desired until the proper amount of reagent is inhaled. There was a hassle to repeat the work. In addition to this, it is necessary to remove the hand blocking the top of the pipette in order to drain the reagent from the pipette, and if it is necessary to drain a small amount of reagent, it is difficult to control the exact amount. Since the inside of the vacuum pipette was exposed to the atmosphere while the mouth was closed from the top of the pipette and then clogged by hand, even though the required amount of reagent was inside the pipette, some of the reagent was discharged from the pipette, making it difficult to accurately remove the reagent. .

As a method for solving such a problem, as shown in FIG. 4B, a mesuring pipette 100b having a rubber nipple 110 inserted therein is used.

The scalpel pipette 100b uses the rubber nipple 100 as follows to relieve the reagent. First, the 'E' cock and the 'S' cock of the rubber nipple 110 are closed, and then the 'A' cock is opened and the rubber nipple 110 is pressed out to release the air therein and then the 'A' cock is closed and the scalpel pipette 100b. ) Into the reagent vessel.

In this state, when the 'S' cock is opened, the rubber nipple 110 is restored to its original shape and the reagent is sucked from the reagent container. When the proper amount of the reagent is sucked into the scalpel pipette 100b, the 'S' cock is closed. do. Then, by pressing the 'E' cock to open the air from the atmosphere into the inside of the rubber nipple 110, the vacuum pressure of the rubber nipple 110 is moved to the atmospheric pressure to allow the reagent to flow out of the scalpel pipette (100b) do. When the reagent has been discharged in the correct amount, it is closed by pressing the 'E' cock again.

However, the scalpel pipette (100b) can be easily used to reduce the reagent using three cocks, but there is an inconvenience to use both hands to adjust the three cocks, especially the size of the scalpel pipette 100 cm or more In the case of large volumetric pipettes, the volume of the rubber nipple is too small, and the inhalation and discharge rate of the reagent is too slow to be suitable for use of the rubber nipple, which requires improvement.

The present invention has been made in view of this point, and the pipette is connected to a mechanism that generates a vacuum pressure, and the reagent is sucked, and the reagents are discharged in a large amount or a small amount from the pipette while removing the vacuum pressure with various control valves. It is possible to use a plurality of pipettes or a pipette having a different diameter at one time, and to inhale / discharge the same or different reagents at the same time by using multiple pipettes. It is possible to provide a precise suction and discharge device of a glass pipette, which is able to discharge the amount of reagent more accurately and consistently, in particular through each control valve.

1 is an overall perspective view showing a precision suction and discharge device according to the invention,

2 is a block diagram showing the configuration of a reagent suction / discharge mechanism according to the present invention;

3 is a bottom perspective view showing the bottom of the vertical transfer apparatus according to the present invention;

4A and 4B show a conventional pipette.

[Description of Symbols for Main Parts of Drawing]

10: reagent suction / discharge mechanism 11: vacuum pump

12 splitter 13 three-way socket

14: vacuum release valve 15: check valve

16: speed control valve 17: hose

18 tube 19 opening and closing valve

20: pipette clamp nipper 21: rubber

30: vertical transfer mechanism 31: base plate

32: guide bar 33: lifting plate

34: pipette holder 35: transfer wheel

36: fixed plate 37: screw bar

38: step motor 39: vertical support

39a, 39b: limit switch 40: movement preventer

100: pipette

100a: one marked pipette

100b: measuring pipette

110: rubber filler (filler)

The present invention for realizing this object is to hold and fix the reagent suction / discharge mechanism for generating a vacuum pressure to suck the reagent and to discharge the reagent by releasing the vacuum pressure, and the pipette connected to the reagent suction / discharge mechanism It is characterized by consisting of a pipette fixing forceps, and a vertical transfer mechanism for supporting the pipette fixing forceps to adjust the height of the pipette.

In a preferred embodiment of the present invention, the reagent suction / discharge mechanism is a vacuum pump, a distributor for selectively distributing at least two or more vacuum pressures generated from the vacuum pump, and removes the vacuum pressure to control the discharge of the reagents A speed control valve, a three-way valve to which the vacuum pump, a distributor, and a control valve are connected, a vacuum pressure release valve to release the vacuum pressure formed inside the vacuum pump, and between the vacuum pump and the three-way valve. It is characterized in that the check valve is installed in the vacuum pump to prevent leakage of the vacuum pressure generated to maintain a constant vacuum pressure.

In addition, the vacuum pump is characterized in that the hand jacket type (hand jacket type).

In addition, the vacuum pressure release valve is characterized in that for removing the vacuum pressure between the vacuum pump and the check valve when opened.

In addition, the check valve prevents the deterioration of fidelity caused by the inflow of air into the vacuum pump at the time of closing, thereby maintaining a stable vacuum pressure between the distributor, the speed control valve and the check valve, and at the same time, the reagent sucked into the pipette at the time of opening. It is characterized in that to discharge a large amount or all the amount.

In addition, the distributor is provided with at least two or more tubes having the same diameter or different diameter so that the pipette can be inserted into the end, each of the tube is characterized in that the opening and closing valve is provided.

In addition, the vertical transfer mechanism is a base plate configured to be movable, at least two or more guide bars erected perpendicularly to the upper portion of the base plate, under the guidance of the guide bar under the control of the step motor to move up and down position by a screw method A lifting plate installed so that the pipette can be fixed, a pipette holder fixed vertically to the center of the lifting plate so that at least two pipette fixing tongs can be adjusted up and down, and a fixing plate fixing the upper end of the guide bar. It is installed vertically between the base plate and the limit switch is provided at both ends, characterized in that composed of a vertical support for limiting the maximum / minimum position of the lifting plate.

Hereinafter, the configuration and operation of the present invention with reference to the accompanying drawings.

1 is an overall perspective view showing a precision suction and discharge device according to the present invention, the reference numeral 100 denotes a pipette.

According to the present invention, the reagent suction / discharge mechanism 10 for suctioning or discharging the reagent by using the pipette 100 through the generation and release of a vacuum pressure, and the pipette 100 to maintain a perpendicular state to the ground Pipette clamping forceps 20 to hold and fix the pipette 100 so that the correct amount can be checked at the time of suction and discharge of the reagents, and the height of the upper and lower sides according to the length of the container or pipette 100 for allowing the reagents to flow out. It is comprised by the up-and-down conveying mechanism 30 which enables adjustment.

As shown in FIGS. 1 and 2, the reagent suction / discharge mechanism 10 includes a vacuum pump 11 for generating a vacuum pressure and a pipette 100 so as to suck the reagent into the reagent 100. A distributor 12 for distributing pneumatic pressure, a speed control valve 16 for controlling the discharge of reagents, a vacuum pump 11, a distributor 12 and a speed control valve 16 for connecting The direction valve 13, the vacuum release valve 14 for releasing the vacuum pressure of the vacuum pump 11, and the check valve used to prevent the vacuum pressure loss generated in the vacuum pump 11 by the air inlet It consists of 15.

In particular, the vacuum pump 11 is a user can easily generate a vacuum pressure by hand, it is preferable to use a hand jacket type (hand jacket type) vacuum pump so that the strength of the vacuum pressure can be easily adjusted arbitrarily, The vacuum pressure release valve 14 of the vacuum pump 11 performs the pressure release of the vacuum pump and the vacuum pressure generated by the operation of the vacuum pump 11 through the check valve 15 is leaked to the tube (or pipette) This will prevent the air from entering the erotic.

In general, when the vacuum pump is left in the standby state after the vacuum pressure is generated, there is a problem that the degree of vacuum drops as air in the air flows into the vacuum pump. In the present invention, the vacuum pump 11 is driven. When the proper vacuum pressure is generated, the check valve 15 is closed, and the vacuum pressure release valve 14 is opened to remove the vacuum pressure between the vacuum pump 11 and the check valve 15 to the vacuum pump 11. It is to prevent the leakage of the vacuum pressure generated when the air is introduced.

In addition, the vacuum pressure release valve 14 and the check valve 15, as described above, serves to prevent the vacuum pressure generated by the vacuum pump 11 from dropping, and to remove reagents in large quantities or completely. Also used for.

That is, the vacuum pressure generated by the vacuum pump 11 is released in accordance with the opening degree of the speed control valve 16 which will be described later while the vacuum degree is maintained by the check valve 15 to release the appropriate amount of reagents. It can be used to reduce the amount of reagents discharged by the speed control valve 16 at a time, or to discharge the remaining reagents in the pipette 100 at the same time to substantially maintain the vacuum pressure By opening the check valve 15 and the vacuum pressure release valve 14 to suck a large amount of air, the vacuum pressure can be removed more quickly, which can be used to discharge or completely remove a large amount of reagents.

Therefore, the vacuum pump 11 generates a vacuum pressure between the distributor 12, the speed control valve 16 and the vacuum pump 11 through the three-way valve 13, the vacuum pressure thus generated As the check valve 15 is closed and the vacuum pressure release valve 14 is opened, the lack of fidelity generated in the vacuum pump 11 (atmospheric air flows into the vacuum pump and the vacuum pressure drops and the inside of the pipette The phenomenon that the solution flows down) is blocked by the check valve (15).

The distributor 12 is connected to the three-way socket 13 to distribute the vacuum pressure generated by the operation of the vacuum pump 11 to the desired pipette 100. In particular, the dispenser 12 is provided with a plurality of tubes 18 (shown in Figure 2 attached to the drawing shown in Figure 2, each having an on-off valve 19 on one side) can be used to connect to the pipette 100. Each of the tubes 18 provides the pipette 100 with a vacuum pressure formed between the distributor 12 and the three-way socket 13 depending on whether the opening / closing valve 19 is opened.

In a preferred embodiment of the present invention, the tube 18 is to be able to use all of the tubes having the same diameter or different diameter, so that it is possible to connect several pipettes at the same time regardless of the type of pipette desirable. Accordingly, the distributor 12 may provide a vacuum pressure to one pipette 100 or several pipettes 100 at the same time according to the opening of the opening / closing valve 19 described above, as well as different diameters. Even if the pipette 100 is used, it is possible to distribute the vacuum pressure to the pipette 100 through the tube 18 corresponding thereto.

Thus, the distributor 12 makes it possible to provide the vacuum pressure generated by the driving of the vacuum pump 11 to the selected one or more tubes 18, thus providing the vacuum provided to the pipette 100 through the rolls of tubes 18. Pneumatic pressure is used to inhale the reagents. The dispenser 12 made in this way is preferably used to be fixed to the pipette holder 34 of the vertical transfer mechanism 30 to be described later.

As described above, when the reagent is sucked into the pipette 100 by using the vacuum pressure distributed through the dispenser 12, the speed control valve 16 controls the discharge and discharge rate of the reagent. do.

The speed control valve 16 is a valve used for fine adjustment in releasing the vacuum pressure, and a rotary valve is used. The speed control valve 16 is rotated about 4 to 8 rotations to introduce atmospheric air to check the distributor 12. The degree of vacuum generated between the valve 15 and the speed control valve 16 is lowered to regulate the discharge of the reagent.

The speed control valve 16, which is a rotary valve type, is discharged in a large amount from the pipette 100 according to the opening degree, that is, by rotating the valve a lot so that a lot of air is supplied to the vacuum degree from the standby state. On the contrary, it is possible to finely control the discharged reagent as little air is supplied by slightly rotating the valve.

As described above, the reagent suction / discharge mechanism 10 uses a hand jacketed vacuum pump 11 to distribute the dispenser 12, the check valve 15, and the speed control via the three-way socket 13. The vacuum pressure is generated between the valves 16, and the vacuum pressure is distributed to the pipette 100 connected through the dispenser 12 to suck the reagents, and the vacuum pressure is released by the speed control valve 16. The aspirated reagents may be discharged in large or small amounts.

The pipette fixing forceps 20 is a forceps used to fix a pipette 100 or a long length pipette, which is commonly used in a laboratory, and as shown in FIG. 1, a pipette holder 34 to be described later. In the preferred embodiment of the present invention, the pipette fixing forceps 20 can be adjusted up and down to fit the length of the pipette 100 by adjusting the fixing position along the length direction of the pipette holder 34. It is used to configure the position adjustment.

The pipette fixing forceps 20 is used to mount one pipette holder 34 in the case of a short pipette 100 in order to stably fix the pipette 100, the pipette 100 more stably It is preferable to use two in pairs, one each at the top and the bottom of the pipette 100 so as to be fixed.

In addition, in a preferred embodiment of the present invention, the pipette fixing forceps 20 is actually attached to the rubber 21 to the inner surface of the forceps to be in contact with the pipette 100, the pipette 100 is a pipette fixing forceps ( 20) it is desirable not to scratch or slip.

The vertical transfer mechanism 30 includes a base plate 31 having a transfer wheel 35 at each corner of the lower surface thereof so as to be movable, a guide bar 32 fixed to an upper surface of the base plate 31, and , A lifting plate 33 which is lifted up and down by a guide of the guide bar 32 by a screw method, a pipette holder 34 fixed to the center of the upper surface of the lifting plate 33, and the guide bar 32. It is composed of a vertical support (39) provided between the fixed plate 36 for fixing the upper end and the base plate 31 to limit the maximum / minimum position of the elevating plate (33).

Here, the base plate 31 is a plate for supporting the reagent suction / discharge mechanism 10 and the vertical transfer mechanism 30 according to the present invention, the lower transfer wheel 35 is provided in the reagent in / It is configured to move freely in the spill place or the laboratory. In addition, the base plate 31 is provided with a movement preventing device 40 to adjust the height up and down by a fixture such as a bolt or screw on the lower surface, the movement preventing device 40 moves the device according to the present invention. If you want to rotate the fixture to adjust the height so that the position slightly higher than the transfer wheel 35 so as not to be caught on the floor during movement, when the suction of the reagent through the apparatus according to the invention by turning the fixture reversely the transfer wheel ( The height of 35 or the height of the wheels to be in a slightly raised position is adjusted so that the base plate 31 is not moved by the transfer wheel 35 during the operation.

1 and 3, the guide bar 32 is attached to the upper surface of the base plate 31 vertically used, as shown in Figure 1 and 3, in particular, each upper end is fixed to the fixed plate 36 have. The fixing plate 36 is formed in a "c" shape, and the four guide bars 32 are fixed to be positioned side by side at each corner portion, and two screw bars are disposed between the four guide bars 32. (37) is mounted side by side facing each other.

The screw bar 37 is mounted on the fixing plate 36 so that the top is rotatable in place and the other end is rotatably supported by the bearing support through the base plate 31. Each end protruding downward is provided on the upper surface of the base plate 31 and is connected to the step motor 38 by which the rotating shaft protrudes downward with a belt.

The elevating plate 33 is a plate member having a predetermined size, and each corner portion thereof is fitted to the guide bar 32 to be slidable up and down, and the screw bar 37 is engaged with the stepper motor 38. It is mounted to move up and down by screwing by driving This lifting plate 33 is limited to the maximum / minimum feed height by the vertical support (39).

The vertical support 39 has a long rod-like shape, both ends of which are flanged and fixed to the base plate 31 and the fixed plate 36. In particular, each of the flanges are provided with limit switches 39a and 39b, and the limit switches 39a and 39b provide a signal to the step motor 38 as the contact is made while raising and lowering the lifting plate 33. To stop the drive, limiting the maximum and minimum lift positions.

The pipette holder 34 is a rod member having a predetermined length, and is vertically mounted at the center of the upper surface of the elevating plate 33. The pipette holder 34 is fixed to the above-described dispenser 12 is installed at the top, and at least one or more pipette fixing forceps 20 to the middle portion of the length to fix the pipette 100 to inhale the reagents do.

Accordingly, the vertical transfer mechanism 30 rotates the screw bar 37 by the driving of the step motor 38 and raises and lowers the elevating plate 33 by the rotation. Accordingly, the pipette holder 34 It is possible to adjust the position up and down to fit the length of the pipette 100 is fixed to the pipette fixing clamp 20 according to the number of the pipette fixing forceps 20 is attached to this at the same time by connecting to the distributor 12 It becomes possible to use.

In a preferred embodiment of the present invention, as mentioned above, a controller is included to control the step motor to move up and down, which includes an ON / OFF switch for supplying power to the step motor 38 and the like. A rising switch and a lowering switch for raising and lowering the elevating plate 33 in accordance with the driving direction of 38 are included. In particular, the rising switch and the lowering switch are provided to the step motor 38 by limit switches 39a and 39b. The circuit is configured to turn off the power selectively.

As described above, the present invention uses a mechanism that generates a vacuum pressure to inhale the reagent and open the valve so that the vacuum pressure is in equilibrium with the atmospheric pressure. By using the connection regardless of the diameter, the following effects are obtained.

1) Using a tube connected to the distributor, it is possible to inhale the reagents in several pipettes and to discharge the reagents in several places at the same time by generating a single vacuum.

2) In addition, the distributor can be used by applying to pipes of different diameters by connecting tubes of different diameters.

3) By controlling the speed control valve, it is possible to control the amount of reagent discharged at a time as much or finely, and it is possible to discharge the reagent safely and accurately.

4) By using the vacuum pressure generated from a simple hand type vacuum pump, it is possible to relieve the reagents and do not need to suck the reagents harmful to the human body, thereby improving safety and convenience.

5) Because the amount of reagent can be finely adjusted, the amount of reagent to be transferred is not transferred properly, eliminating the inconvenience of making a sample several times.

Claims (8)

A reagent suction / exhaust mechanism for generating a vacuum pressure to suck the reagent and releasing the vacuum pressure to discharge the reagent, a pipette connected to and supported by the reagent suction / discharge mechanism, and holding the reagent, and moving the pipette up and down In the suction and discharge device of the glass pipette, The reagent suction / discharge mechanism includes a vacuum pump, a distributor for selectively distributing at least two or more vacuum pressures generated from the vacuum pump, a speed control valve for removing the vacuum pressure to control the discharge of the reagent, and the vacuum A three-way valve to which the pump, the distributor, and the speed control valve are respectively connected, a vacuum pressure release valve for releasing the vacuum pressure formed inside the vacuum pump, and a vacuum pump and the three-way valve installed between the vacuum pump and the three-way valve Precision suction and discharge device of the glass pipette, characterized in that made of a check valve to prevent the leakage of vacuum pressure to maintain a constant vacuum pressure. delete The device of claim 1, wherein the vacuum pump is of a hand jacket type. The device of claim 1 or 3, wherein the vacuum pump is integrated with a vacuum release valve. The apparatus of claim 1, wherein the vacuum pressure release valve removes a vacuum pressure between the vacuum pump and the check valve at the time of opening. The check valve of claim 1, wherein the check valve prevents a decrease in fidelity caused by inflow of air into the vacuum pump during closing, thereby stably maintaining a vacuum pressure between the distributor, the speed control valve, and the check valve, and suctioning the pipette during opening. Precision suction and discharge device of the glass pipette, characterized in that to discharge a large amount or all of the reagents at once. delete delete