KR100391002B1 - Reutilization method of Korean paper digestion wastewater as the substrate for cultivating yeast - Google Patents

Abstract

The present invention relates to a method for reusing a Korean boiled waste liquor as a culture substrate of yeast, and is composed of a pretreatment method and a culture method of cooked waste. The pretreatment method of self-cleaning waste liquid is dilution of the waste liquid with water, and then the pH is adjusted and filtered using an acid. The culturing method is to inoculate the yeast in the pretreatment solution and incubate to increase the bacterial concentration by adding an appropriate amount of self-stock preparation at an appropriate time.

Description

Reutilization method of Korean paper digestion wastewater as the substrate for cultivating yeast}

The first stage of the Hanji manufacturing process is the ripening of the bark of bark with sodium hydroxide. In the ripening process, since non-fiber and fiber contained in the bark are hydrolyzed and eluted by sodium hydroxide, the cooking waste may contain various nutrients necessary for the growth of microorganisms. Therefore, it can be used as a culture substrate of microorganisms, and it is thought that it can be a particularly good culture substrate of yeast because various sugar components decomposed and eluted from hemicellulose and cellulose are contained in the self-cultivation solution. In view of this, the present invention was attempted to create new added value in reusing waste paper treatment waste of Hanji manufacturing plant by reusing it as a culture substrate of yeast.

The technique of using sulfurous acid cooked liquor similar to the Korean paper sleeping liquor as a culture substrate of yeast has been studied.

There is no particularly difficult technical problem to be solved by the present invention, but two technical problems have been solved in the process of the present invention. First, yeast did not fully grow in the Korean paper boiled liquor liquor, which was due to osmotic pressure. Second, the dilution of self-cleaning waste decreased the microbial nutrients in the waste, thus reducing the maximum growth of yeast. It was possible to increase the maximum growth rate by adding the cooking stock solution.

1 is a block diagram of the present invention

Figure 2 is a result of applying the present invention to feed additive yeast

The configuration of the present invention is as shown in FIG. That is, first, the stock solution 101 of the Korean paper boiled waste liquid is diluted (102) with an appropriate dilution, and then, an acid solution is added to the diluted solution (103) to adjust the propagation titration pH range of the yeast and filtered (104). Next, after sterilization of the filtrate (105), inoculation (106) of the yeast is cultured (107). Next, during the cultivation, an appropriate amount of the cooked crude solution, which is adjusted to the same pH as the culture solution and filtered, is added 108 at an appropriate time to continuously culture 109 until the yeast grows to the maximum. The present invention has been applied to feed additive yeast, because of the fact that considering the market scale, market stability, cultivation and ease of production among the yeast products, the product having the greatest potential for economic production using the self-added waste solution is the feed additive yeast. Because it was judged. Therefore, the yeast used pure yeast isolated from CYC 100, a feed additive yeast agent of Joongang Chemical Co., Ltd. Application conditions are as follows. That is, the distilled water was diluted with distilled water to 7.5 times the appropriate dilution degree, and adjusted to pH 4.0 by adding sulfuric acid to the diluent and filtered through No. 5C filter paper. 50 ml of the filtrate was placed in a 250 ml triangle flask, autoclaved at 121 ° C. for 15 minutes, inoculated with a yeast suspension, and incubated at 30 ° C. and 180 rpm using a shaking constant temperature bath. The results of the practice of not adding the self-stock solution during the cultivation and the experimental results of the addition of 5 ml of the self-stock solution adjusted to pH 4.0 using sulfuric acid solution at 14 hours of cultivation are shown in FIG. 2. Compare with each other. As shown in FIG. 2, when the cooking stock solution is not added during the cultivation (201), the total bacterial count of yeast is 1.1 × 10 ⁷ / ml, which is the maximum value after 26 hours of incubation at the initial 2.8 × 10 ⁵ / ml (202). Was reached (203). Therefore, yeast was grown to about 10 ⁷ / ml in total dilution of Hanji porridge. In the case of the addition of the raw cooking stock (204), the growth was continued (205) after 26 hours at the initial 2.8 × 10 ⁵ / ml (202), reaching 7.4 × 10 ⁷ / ml in 39 hours of culture (206). Therefore, by adding the self-stock solution during the cultivation, it was possible to increase the number of bacteria about 6.7 times more than without.

The present invention can be used to produce the yeast-related products by reusing the Hanjijaja wastewater as a culture substrate of yeast can be expected to create a new value added. As a paper maker, wastewater treatment costs are reduced, improving competitiveness. As it can be used as a culture substrate of other microorganisms such as yeast as well as yeast, it can be expected to reduce raw material cost when used as a raw material for producing various microbial products.

Claims (1)

In the process of reusing Hanji porridge waste as a culture substrate of yeast, in particular, diluting the porcelain waste solution and increasing the concentration of fungi by adding the porcelain solution during inoculation with yeast