KR100380759B1 - Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used - Google Patents

Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used Download PDF

Info

Publication number
KR100380759B1
KR100380759B1 KR10-2000-0083118A KR20000083118A KR100380759B1 KR 100380759 B1 KR100380759 B1 KR 100380759B1 KR 20000083118 A KR20000083118 A KR 20000083118A KR 100380759 B1 KR100380759 B1 KR 100380759B1
Authority
KR
South Korea
Prior art keywords
falcarindiol
antifungal
japonicum
medicinal plant
present
Prior art date
Application number
KR10-2000-0083118A
Other languages
Korean (ko)
Other versions
KR20020053474A (en
Inventor
배영석
Original Assignee
경상북도 농업기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 경상북도 농업기술원 filed Critical 경상북도 농업기술원
Priority to KR10-2000-0083118A priority Critical patent/KR100380759B1/en
Publication of KR20020053474A publication Critical patent/KR20020053474A/en
Application granted granted Critical
Publication of KR100380759B1 publication Critical patent/KR100380759B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Medical Informatics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

본 발명은 각종 방부제와 진균에 의한 인체감염을 치료할 수 있는 약제성분, 즉 항진균제를 우리나라 약용식물인 식방풍(학명 :Peucedanum japonicum)으로부터 추출정제하여 사용케 하므로서 항진균제의 수입에 따른 외화유출방지와 국내약용식물 재배농가의 수익성을 기대할 수 있도록 한 것이다.The present invention allows the use of various preservatives and fungicides that can treat human infections by fungi, namely antifungal agents, by extracting and using them from the Korean medicinal plant, the edible breeze ( Pucedanum japonicum ), and preventing foreign currency leakage according to the import of antifungal agents. The profitability of medicinal plant farmers can be expected.

상기 목적을 달성하기 위하여 본 발명은 약용식물인 식방풍(Peucedanum japonicum)으로부터 팔카린디올(falcarindiol)을 정제하고, 상기 팔카린디올 (falcarindiol)을 효모의 생육을 저해하는 항진균제로 사용케 한 것이다.In order to achieve the above object, the present invention is to purify falcarindiol (Palcarindiol) from the medicinal plant, Peucedanum japonicum , and to use the falcarindiol as an antifungal agent to inhibit the growth of yeast.

Description

식방풍으로부터 추출정제한 팔카린디올을 이용한 항진균제{Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used}Antifungal Agent Using Falcarindiol Extracted from Dietary Wind {Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used}

본 발명은 각종 방부제와 진균에 의한 인체감염을 치료할 수 있는 약제성분, 즉 항진균제를 약용식물인 식방풍(Peucedanum japonicum)으로부터 추출정제하여 사용할 수 있도록 한 것이다.The present invention is to be used to extract and tablet pharmaceutical ingredients that can treat human infection by various preservatives and fungi, that is, antifungal agents from the medicinal plant, Peucedanum japonicum .

일반적으로 미생물에 의한 식품부패 방지 및 진균에 의한 인체감염등을 치료하기 위한 항진균제들은 전량수입에 의존하고 있는 실정이므로 항진균제 수입에 따른 외화의 낭비가 많아지게 된다.In general, antifungal agents for preventing food corruption caused by microorganisms and treating human infections caused by fungi have a large amount of foreign currency due to the import of antifungal drugs because the antifungal agents rely on imports.

본 발명은 이러한 종래의 제반단점을 시정하고자 항진균제를 제공함에 있어서, 국내 농가에서 재배 가능한 약용식물로부터 추출정제하여 사용할 수 있도록 한것이다.The present invention is to provide an antifungal agent to correct the above-mentioned general disadvantages, to be used to extract and purified from medicinal plants cultivable in domestic farms.

현재까지 팔카린디올의 항진균효능이 조사된 적이 없었다.To date, the antifungal efficacy of falcarindiol has not been investigated.

본 발명은 국내농가에서 재배가능한 식방풍(Peucedanum japonicum)으로부터 팔카린디올(falcarindiol)을 추출정제하고, 상기 팔카린디올(falcarindiol)을 항진균제로 사용케 한 것이다.The present invention is to extract and purify falcarindiol (Palcarindiol) from the cultivation wind ( Pucedanum japonicum ) cultivable in domestic farms, and to use the falcarindiol as an antifungal agent.

상기 목적을 달성하기 위하여 본 발명은 약용식물인 식방풍(학명 :Peucedanum japonicum)을 분쇄하여 분말을 얻고, 상기 분말을 메탄올(methanol)과 혼합교반하여 추출액을 얻고, 상기 추출액을 감압, 농축, 건조한 다음 에칠아세테이트를 첨가하여 그 중 녹는 성분을 분리시킨후 농축건조하고, 상기 시료를 클로로포름메탄올용액에 녹여 실리카겔 칼럼(Silicagel column)에 흡착시킨 후 흡착된 물질을 클로로포름 메탄올의 부피비를 점차 달리하면서 매우 느린 유속으로 용출시켜 일정량씩 분획하고, 상기 분획시료 중 토포이소메라제Ⅰ(topoisomerase Ⅰ)저해활성을 강하게 나타내는 분획을 모아 실리카겔 칼럼에 다시 한번 더 흡착시킨 후 흡착된 물질을 헥산(hexane)과 에칠아세테이트의 혼합용매로 재용출, 분획하고, 상기 분획시료 중 토포이소메라제Ⅰ 저해활성을 강하게 나타내는 분획을 모아 정상 박층크로마토그라피(normal phase thin layer chromatography)판에 침적한 후 헥산(hexane), 에칠아세테이트 초산(acctic acid)의 용매로 전개하여 저해성분을 분리하고, 저해성분을 포함하는 실리카(Silica)를 박층 크로마토그라피판으로부터 긁어내어 에칠아세테이트로 용출하고, 상기 용출액을 각 바이알(vial)에 분취하여 감압회전건조기로 건조하여 본 발명에서 얻고자 하는 팔카린디올(falcarindiol)을얻고, 상기 팔카린디올(falcarindiol)을 항진균제로 사용케 하는 것이다.In order to achieve the above object, the present invention obtains a powder by grinding the medicinal plant wind ( Pucedanum japonicum ), the mixture is stirred with a mixture of methanol (methanol) to obtain an extract, the extract is reduced pressure, concentrated, dried Next, ethyl acetate was added to separate the soluble components, and the resultant was concentrated and dried. The sample was dissolved in chloroformmethanol solution and adsorbed onto a silica gel column, and the adsorbed material was gradually changed in volume ratio of chloroform methanol. After eluting at a flow rate, the fractions were fractionated. The fractions showing strong inhibitory activity of topoisomerase I in the fraction sample were collected and adsorbed on the silica gel column once more, and then the adsorbed material was added to hexane and ethyl acetate. Redissolved and fractionated with a mixed solvent of toluene, and enhanced topoisomerase I inhibitory activity in the fractionated sample. The collected fractions were collected, deposited on a normal phase thin layer chromatography plate, and then developed with a solvent of hexane and acetylacetate acetic acid to separate the inhibitors, and silica containing the inhibitors. (Silica) was scraped off from the thin layer chromatography and eluted with ethyl acetate. The eluate was aliquoted into each vial and dried with a vacuum rotary dryer to obtain falcarindiol to be obtained in the present invention. Falcarindiol can be used as an antifungal agent.

도 1 : 본 발명의 항진균제로 사용하기 위한 팔카린디올의 제조공정도1: Process diagram of preparation of falcarindiol for use as an antifungal agent of the present invention

도 2 : 본 발명에서 얻어진 팔카린디올이 미생물생육도에 미치는 영향을 나타낸 도표2 is a diagram showing the effect of falcarindiol obtained in the present invention on the microbial growth

이하 본 발명에서 제공하고자 하는 항진균제로 사용가능한 팔카린디올 (falcarindiol)을 약용식물인 식방풍(Peucedanum japonicum)으로부터 정제하는 실시예를 상세히 설명하면 다음과 같다.Hereinafter, an embodiment of purifying Falcarindiol usable as an antifungal agent to be provided in the present invention from Peucedanum japonicum as a medicinal plant will be described in detail.

약용식물인 식방풍(Peucedanum japonicum)가루 4Kg과 메탄올(methanol)20ℓ를 섞어 충분히 교반하여 유효성분을 추출한 후 침전물을 버리고, 추출액을 회전증발기로 감압농축하여 건조시킨다.4Kg of powdered medicinal plant (Peucedanum japonicum) and 20 liters of methanol (methanol) are mixed and sufficiently stirred to extract the active ingredient, then the precipitate is discarded. The extract is concentrated under reduced pressure with a rotary evaporator and dried.

상기 건조된 시료에 에칠아세테이트(ethylacetate) 500 밀리리터를 첨가하면 녹는 성분과 녹지 않는 성분 두가지로 나누어지게 되며, 이때 에칠아세테이트에 녹는 성분을 취하여 다시 농축건조시킨다.When 500 milliliters of ethylacetate is added to the dried sample, it is divided into two components, a soluble component and a non-soluble component. At this time, the component dissolved in the ethyl acetate is taken and concentrated to dry again.

에칠아세테이트에 녹는 성분을 취하여 농축건조한 시료를 클로로포름 : 메탄올(CHCl3: MeOH)(50 : 1)의 용액 10㎖에 녹여 실리카겔(silicagel)칼럼 (5.8 ×67cm)에 흡착시킨 후 흡착된 물질을 클로로포름 : 메탄올의 부피비를 40 : 1, 35 : 1, 30 : 1, 25 : 1, 20 : 1로 점차 달리하면서 분당 0.5㎖의 느린 유속으로 용출시켜 20㎖씩 분획한다.The sample dissolved in ethyl acetate was concentrated, and the dried sample was dissolved in 10 ml of a solution of chloroform: methanol (CHCl 3 : MeOH) (50: 1), adsorbed onto a silica gel column (5.8 x 67 cm), and the adsorbed material was then chloroform. The volume ratio of methanol is 40: 1, 35: 1, 30: 1, 25: 1, 20: 1 while eluting at a slow flow rate of 0.5 ml per minute and fractionating 20 ml.

토포이소메라제Ⅰ(topoisomeraseⅠ) 저해활성을 강하게 나타내는 분획을 모아 실리카겔 칼럼(3 ×70cm)에 다시 한번 더 흡착시킨 후 흡착된 물질을 헥산(hexane) : 에칠아세테이트(ethylacetate)를 3 : 1의 혼합비로 혼합한 용매로상기와 같은 방법으로서 재용출시키면서 분획한다.The fractions showing strong topoisomerase I inhibitory activity were collected and adsorbed again on the silica gel column (3 × 70 cm), and the adsorbed material was mixed with hexane (ethylacetate) in a ratio of 3: 1. Fractions while re-eluting with a solvent mixed with the same method as above.

상기 분획중에서 토포이소메라제Ⅰ(topoisomerase Ⅰ)에 대하여 저해활성을 강하게 나타내는 분획을 모아 정상 박층크로마토그라피(normal phase thin layer chromatography)판에 집적한 후 헥산(hexane), 에칠아세테이트, 초산(aceticacid) (30 : 20 : 1)의 용매로 전개하여 저해성분을 분리하고, 저해성분을 포함하는 실리카(silica)를 박층 크로마토그라피판으로부터 긁어내어 에칠아세테이트로 용출한다.Of the fractions, fractions showing strong inhibitory activity against topoisomerase I were collected and integrated on a normal phase thin layer chromatography plate, followed by hexane, ethyl acetate, and acetic acid. It develops with the solvent of (30: 20: 1), isolate | separates an inhibitor, and silicas containing an inhibitor is scraped off from a thin layer chromatography, and eluted with ethyl acetate.

상기 용출액을 각 바이알(vial)에 분취하여 감압회전건조기로 45℃에서 농축건조하므로서 본 발명에서 제공받고자 하는 팔카린디올(falcarindiol) 약200mg을 얻을수 있었으며 분석결과 구조식은 아래와 같이 분석되었다.The eluate was collected in each vial and concentrated to dryness at 45 ° C. with a vacuum rotary dryer to obtain about 200 mg of falcarindiol to be provided in the present invention. The structural formula was analyzed as follows.

상기에서와 같이 약용식물인 식방풍(Peucedanum japonicum)으로부터 추출정제한 팔카린디올(falcarindiol)이 암세포 뿐만 아니라 효모에 대한 항균력이 우수하다는 사실을 본 발명에서 확인할 수 있었으며 본 발명의 팔카린디올을 이용하여식품보존등을 위한 항진균제로의 사용도 가능한 것으로 판단되었다.As described above, Falcarindiol extracted and purified from the medicinal plant Peucedanum japonicum (falcarindiol) can be confirmed in the present invention that the antimicrobial activity as well as cancer cells as well as using the falcarindiol of the present invention Therefore, it could be used as an antifungal agent for food preservation.

식방풍(Peucedanum japonicum)으로부터 추출정제한 팔카린디올 (falcarindiol)이 미생물에 미치는 효과를 조사하였다.The effect of falcarindiol extracted and purified from Peucedanum japonicum on microorganisms was investigated.

미생물의 생육도는 대장균(E.Coli)과 효모인 사카로마이세스 세레비시아(S. cerevisiae)를 LB배지와 YPAD배지에서 각각 24시간 배양한 후 600nm(나노미터)에서 흡광도를 조사함으로써 그 생육도를 조사하였다.The growth of microorganisms was determined by incubating E. coli and yeast S. cerevisiae in LB medium and YPAD medium for 24 hours, and then absorbance at 600 nm (nanometer). The growth rate was examined.

이때 배지에 팔카린디올 농도별(0∼25㎍/㎖)로 첨가하고, 대장균과 사카로마이세스 세레비시아(S.cerevisiae)의 생육저해정도를 비교한 결과, 사카로마이세스 세레비시어(S. cerevisiae)의 생육도는 팔카린디올의 량이 증가함에 따라 현저히 감소하였다(도 2).Saccharomyces cerevisiae was added to the medium by concentration of Falcarindiol (0-25 ㎍ / ml), and the growth inhibition of Escherichia coli and S. cerevisiae was compared. Growth of (S. cerevisiae) was significantly decreased as the amount of falcarindiol increased (Fig. 2).

그래서 사카로마이세스 세레비시아에 대한 팔카린디올의 IC50값은 9㎍/㎖이었다.Thus, the IC 50 value of Falcarindiol against Saccharomyces cerevisiae was 9 μg / ml.

이결과 팔카린디올(falcarindiol)이 식품보존등을 위한 항진균제로 사용될 수 있는 것으로 판단되었다.As a result, it was determined that falcarindiol could be used as an antifungal agent for food preservation.

이상과 같이 본 발명은 약용식물인 식방풍으로부터 효모에 대한 항균력이 우수한 팔카린디올(falcarindiol)을 추출한 다음 항진균제로 사용케 하므로서 항진균제에 대한 대체효과가 있을 뿐 아니라 식방풍과 같은 약용식물을 농촌에 보급, 재배케 하므로서 농가소득에 기여할 수 있게 되는 등의 효과가 있는 것이다.As described above, the present invention extracts falcarindiol having excellent antibacterial activity against yeast from the medicinal plant wind, and then uses it as an antifungal agent. By spreading and cultivating, it is possible to contribute to farm income.

Claims (1)

식방풍(Peucedanum japonicum)으로부터 팔카린디올(falcarindiol)을 추출하고, 상기 팔카린디올을 미생물의 항진균제로 사용함을 특징으로 하는 식방풍 (Peucedanum japonicum)으로부터 추출정제한 팔카린디올(falcarindiol)을 이용한 항진균제.Extract Karin diol (falcarindiol) arm from the formula windproof (Peucedanum japonicum), and with the wind guard arm Karin diol (falcarindiol) extracted and purified from (Peucedanum japonicum) expression, characterized by using the arm Karin diol of microbial antifungal antifungal .
KR10-2000-0083118A 2000-12-27 2000-12-27 Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used KR100380759B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR10-2000-0083118A KR100380759B1 (en) 2000-12-27 2000-12-27 Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR10-2000-0083118A KR100380759B1 (en) 2000-12-27 2000-12-27 Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used

Publications (2)

Publication Number Publication Date
KR20020053474A KR20020053474A (en) 2002-07-05
KR100380759B1 true KR100380759B1 (en) 2003-04-18

Family

ID=27686839

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2000-0083118A KR100380759B1 (en) 2000-12-27 2000-12-27 Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used

Country Status (1)

Country Link
KR (1) KR100380759B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101440830B1 (en) * 2013-03-21 2014-09-17 원광대학교산학협력단 A composition for preventing and treating bone diseases containing peucedani japonici radix extracts as a active ingredient

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455278A (en) * 1994-09-19 1995-10-03 Wisconsin Alumni Research Foundation Method of inhibiting pathogens and food spoilage bacteria
JPH11310527A (en) * 1998-02-25 1999-11-09 Nomura:Kk Pharmaceutical composition

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455278A (en) * 1994-09-19 1995-10-03 Wisconsin Alumni Research Foundation Method of inhibiting pathogens and food spoilage bacteria
JPH11310527A (en) * 1998-02-25 1999-11-09 Nomura:Kk Pharmaceutical composition

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
1쪽 요약, Biosci Biotechnol Biochem, 1996 Jan, 60(1), 152~153 *
1쪽 요약, Planta Med, 1996 Jun, 62(3), 256~259 *

Also Published As

Publication number Publication date
KR20020053474A (en) 2002-07-05

Similar Documents

Publication Publication Date Title
CN101454015A (en) Extracts and methods comprising green tea species
Sultan Chromatographic separation and identification of many fatty acids and phenolic compounds from flowers of Celosia cristata L. and its inhibitory effect on some pathogenic bacteria
US4254112A (en) Inula extract, its method of preparation and its use as pharmaceutical
Rangama et al. Antimicrobial activity of Tephrosia purpurea (Linn.) Pers. and Mimusops elengi (Linn.) against some clinical bacterial isolates
CN101209952B (en) Sesquiterpenoids, composition thereof, method for extracting the same from plants and application
Chokki et al. Phytochemical screening and antimicrobial activity of Momordica charantia L. and Morinda lucida Benth extracts from Benin
Okwu et al. Isolation, characterization and antibacterial activity screening of anthocyanidine glycosides from Alchornea cordifolia (Schumach. and Thonn.) Mull. Arg. leaves
CN100491364C (en) Sesquiterplactone in dandelion and its use of resisting Gram's positive bacteria
KR100380759B1 (en) Antifungal which the Falcarindiol isolated from Peucedanum japonicum is used
Aswar et al. Antimicrobial activity of Ficus benghalensis
CN113413341B (en) Plant source compound bacteriostatic agent
KR101211669B1 (en) Antimicrobial compounds from Scilla scilloides and composition containing thereof
Reid et al. Isolation of the anti-bacterial vernodalin from traditionally used Vernonia colorata
Ushiki et al. Medicinal plants for suppressing soil-borne plant diseases: II. Suppressive effect of geranium pratense l. On common scab of potato and identification of the active compound
CN100471851C (en) Lignin in dandelion, its bacteria-resisting activity and use for medicine
Akpan et al. Biochemical profiling of leaf, stem bark and root of Enantia chlorantha native to Akwa Ibom, Nigeria: A comparative analysis
KR20130076114A (en) Composition comprising extract of chrysanthemum zawadskii and aronia mandschurica
FI82878C (en) FUNGISTATISK FOERFARANDE.
CN113209161B (en) Sinomenium acutum fruit extract and preparation method and application thereof
CN106912489A (en) The preparation method and applications of AVM analog derivative
RU2691997C1 (en) Method of producing a substance exhibiting antifungal activity
JP4652820B2 (en) Novel nitrile glycosides useful as bioenhancers for drugs and nutrients and methods of isolation from Moringoleifera
KR101784503B1 (en) ANTI-IMPOTENCE DRUG CONTAINING β-sitosterol-6'-linolenoyl-3-O-β-D-glucopyranoside EXTRACTED FROM LESPEDEZA CUNEATA, AND FUNCTIONAL FOODS FOR SAME
KR20240065782A (en) A cosmetic composition containing the cyclohaptadriene derivative isolated from the algae flower extract and the cyclohaptadriene derivative isolated from the algae flower extract
Sabitha et al. DETECTION AND ESTIMATION OF RUTIN, QUERCETIN, GALLIC ACID AND MANGIFERIN IN MELIACEAE, PIPERACEAE, AND ROSACEAE FAMILY BY HPTLC TECHNIQUE

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20070327

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee