KR100358752B1 - A monitor ampule for quality display of frozen semen of cow - Google Patents

Abstract

The present invention relates to a copper crystal liquid quality display device, and more particularly, to an apparatus for informing whether there is an abnormality in quality depending on the storage state of the copper crystal liquid.

Accordingly, the copper crystal liquid quality display device of the present invention is configured to check the storage state by using three ampoules that melt according to temperature.

Description

A MONITOR AMPULE FOR QUALITY DISPLAY OF FROZEN SEMEN OF COW}

The present invention relates to a copper crystal liquid quality display device, and more particularly, to an apparatus for informing whether there is an abnormality in quality depending on the storage state of the copper crystal liquid.

Accordingly, the copper crystal liquid quality display device of the present invention is configured to check the storage state by using three ampoules that melt according to temperature.

As is well known to those skilled in the art, copper crystals stored in liquid nitrogen (-196 ° C.) may be used for various exposures such as the number of air exposures, liquid filling conditions, and liquid nitrogen storage locations according to the use of copper crystals during the sustained microcirculation movement and distribution. Depending on the conditions, quality deterioration occurs. In general, liquid nitrogen is supplied about once a month, so that nitrogen is not always sufficiently filled in the nitrogen tank and is easily exposed to the outside air during distribution, resulting in deterioration of the quality of the crystalline liquid.

As such, when the copper crystal solution is exposed, the survival rate of the copper crystal solution of 54.5% decreases when exposed to -35 ℃ 26.9% when exposed to -100 ℃.

In order to indicate the survival rate of the copper crystal solution, conventionally, a method of melting the copper crystal solution and inspecting it under a microscope was used. However, the copper crystal solution must be discarded every time it is actually inspected, and the economic loss caused by the purchase of a microscope and other equipment, and a lot of time. This consumed problem has existed.

As an alternative, there is a display device using two ampoules. The display device is composed of an ampoule containing a blue reagent melting at -100 ° C and an ampoule containing a red reagent melting at -35 ° C.

As shown in FIG. 1, the state of the frozen semen by the apparatus is determined to be normal when the reagents contained in the two ampoules 100 and 110 are not melted, and the blue reagent is classified as shown in FIG. 2. If there is an ampoule that is melted under the reference position 102, it is determined that it is necessary to examine the copper crystal solution, and as shown in FIG. 3, all reagents contained in the two ampoules are melted under the state classification reference positions 102 and 112. If present, it indicates that the copper crystal solution should be discarded.

However, the display device as described above has a problem that it is not known whether the storage device is stored at a temperature between -160 ° C and -196 ° C, which has a low influence on the viability of the copper crystal solution.

SUMMARY OF THE INVENTION The present invention has been made to solve the problems of the prior art operating as described above, and a main object of the present invention is to provide a display device capable of confirming whether a copper crystal solution is stored only at the best storage temperature.

1 is a state diagram of the indicator when the frozen semen according to the prior art is normal,

2 is a state diagram of the indicator when the inspection of the frozen semen according to the prior art is necessary,

3 is an indicator state diagram when the frozen semen in the prior art should be discarded,

4 is an indicator state diagram when the frozen sperm according to the present invention is normal,

5 is an indicator state diagram when the frozen semen according to the present invention needs attention,

6 is a state diagram of the indicator when the state of the frozen semen according to the present invention needs a warning,

7 is an indicator state diagram when the frozen semen in accordance with the present invention should be discarded,

8 is a state diagram when the indicator according to the present invention is mounted on the canister.

<Explanation of symbols for the main parts of the drawings>

100: blue ampoule 102: blue ampoule status classification reference position

110: red ampoule 112: red ampoule status classification reference position

120: blue ampoule 122: blue ampoule status classification reference position

130: yellow ampoule 132: yellow ampoule status classification reference position

140: red ampoule 142: red ampoule status classification reference position

150: canister

BRIEF DESCRIPTION OF THE DRAWINGS The present invention will be more apparent from the following detailed description based on the accompanying drawings in order to achieve the above object.

In the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted. Terms to be described later are terms defined in consideration of functions in the present invention, and may be changed according to intentions or customs of users or operators. Therefore, the definition should be made based on the contents throughout the specification.

An apparatus for storing copper crystal quality of the present invention includes a first ampoule 120 containing a reagent that melts at -160 ° C ± 10 ° C in a copper crystal storage canister for storing frozen semen. In one embodiment, the reagent of the first ampoule is preferably blue in color. In addition, the reagent at this time may be, for example, isopentan.

In addition, the same crystal storage canister for storing frozen semen, and includes a second ampoule 130 containing a reagent that is melted at -100 ℃ ± 10 ℃. In one embodiment, the reagent of the second ampoule is preferably yellow in color. In this case, the reagent inserted into the second ampoule may be, for example, nitroethane, heptane, n-propyl acetate, 2-nitropropane, or cyclopentane. Cyclopentane, Ethylbenzene, Hexane, Acetone, Toluene, Cumene, Methanol, Methyl acetate Isobutyl acetate, Cyclo It is preferable to use one of hexane (Cyclohexane), isooctane, 1-nitropropane, ethanol (Ethanol), ethyl ether, and isoamyl alcohol.

In addition, the same crystal storage canister for storing the frozen semen, and includes a third ampoule (140) containing a reagent that is melted at -35 ℃ ± 10 ℃. In one embodiment, the reagent of the third ampoule is preferably red in color. In this case, the reagent inserted into the third ampoule may be, for example, dimethyl sulphate, 1,3-dichlorobenzene, xylene, or bromobenzene. ), M-Toluidine, Dipropyl ketone, Thiophen, Thiopen, Methyl cyanide, Pyridine, Cyclohexyl chloride, Chlorobenzene ( Chlorobenzene), ethylene glycol (Ethylene glycol), polyethylene glycol (Polyethylene glycol), glycerol (Glycerol) can be used one.

Referring to the accompanying drawings, the copper crystal liquid quality display device according to the present invention configured as described above is as follows.

The copper crystal liquid quality display device according to the present invention is stored in the canister together with the copper crystal liquid. In this embodiment, one copper crystal liquid quality display device may be inserted and managed per 100 canisters. Afterwards, if the storage environment is kept below -160 ℃ ± 10 ℃, all the ampoule's reagents are not fused and the manager can know that there is no problem in the crystallization solution.

After that, when the copper crystal solution is exposed between -160 ℃ ± 10 ℃ and -100 ℃ ± 10 ℃, the blue reagent is melted and melted below the standard position 122 of the blue ampoule. It can be seen that care must be taken.

Since the storage environment is changed and the copper crystal solution is exposed between -100 ℃ ± 10 ℃ and -35 ℃ ± 10 ℃, the blue and yellow reagents are melted and melted under the state classification reference position (122) (132) of the ampoule. It can warn the user of poor storage status.

After that, when the copper crystal solution is exposed to a temperature above -35 ℃ ± 10 ℃ and all the reagents are melted, all the reagents are melted and melted under the reference position (122) (132) (142). You will know that you need to discard semen.

The present invention can be variously modified and can take various forms and only the specific embodiments thereof are described in the detailed description of the invention. It is to be understood, however, that the present invention is not limited to the specific forms referred to in the description, but rather includes all modifications, equivalents, and substitutions within the spirit and scope of the invention as defined by the appended claims. Should be.

According to the present invention, it is obvious that the manager can know the state of the copper crystal solution in more detail, thereby effectively managing the distribution process of the copper crystal solution, and it is a very advantageous invention that can prevent the artificial fertilization rate of the livestock from being lowered due to the use of the defective crystal solution. .

Claims (7)

In the same crystal storage canister for storing the frozen semen, A first ampoule containing a reagent that melts at −160 ° C. ± 10 ° C .; A second ampoule containing a reagent that melts at −100 ° C. ± 10 ° C .; A copper crystal quality indicator comprising a third ampoule containing a reagent that melts at -35 ° C ± 10 ° C. The method according to claim 1, The copper crystal liquid quality display device of claim 1, wherein the reagent of the first ampoule is blue. The method according to claim 1, And a reagent inserted into the first ampoule is isopentan. The method according to claim 1, The copper crystal solution quality indicator of the second ampoule has a yellow color. The method according to claim 1, Reagents inserted into the second ampoule may include nitroethane, heptane, n-propane acetate, 2-nitropropane, 2-cyclopropane, cyclopentane, and ethylbenzene. Ethylbenzene, Hexane, Acetone, Toluene, Cumene, Methanol, Methyl acetate, Isobutyl acetate, Cyclohexane, Isooctane (Isooctane), 1-nitropropane (1-Nitropropane), ethanol (Ethanol), ethyl ether (Ethyl ether), isoamyl alcohol is one of the freezing semen quality display device. The method according to claim 1, And a reagent of the third ampoule having a red color. The method according to claim 1, Reagents inserted into the third ampoule include dimethyl sulphate, 1,3-dichlorobenzene, xylene, bromobenzene, and m-tolidine ( m-Toluidine, Dipropyl ketone, Thiophen, Thiopen, Methyl cyanide, Pyridine, Cyclohexyl chloride, Chlorobenzene, Ethylene glycol Bovine frozen semen quality display device of one of glycol, polyethylene glycol, and glycerol.