KR100340750B1 - A manufacturing process of the functional hydrolystates of chitosan using microwave - Google Patents

Abstract

PURPOSE: A process for preparing functional chitosan hydrolysate having antibiotic activity using microwave is provided, thereby cheaply preparing the functional chitosan hydrolysate in higher yield. CONSTITUTION: A process for preparing functional chitosan hydrolysate having antibiotic activity using microwave comprises the steps of: dissolving 0.5g of harmless organic acids including succinic acid, lactic acid and maleic acid in 100 ml of water; adding 1 to 2 g of chitosan into the solution; and heating the chitosan added solution in a microwave digestion system(USA) at 121 deg. C for 200 minutes.

Description

A manufacturing process of the functional hydrolystates of chitosan using microwave}

The present invention is a functional chitosan hydrolyzate using microwave which can be applied to various food industries with economical and environmentally friendly acid decomposition method in manufacturing chitosan hydrolyzate using chitosan prepared from chitin extracted from shell of crab crustacean It relates to a manufacturing method of.

Chitosan is produced from chitin and which is extracted from the bark of gedeung crustacean was once in the digestion process is treated as a failed useful substances without biological physiology wateuna are left with unused resources, come in recent years, many studies, key Latin and chitosan Is a non-toxic substance with adsorption, moisturizing, emulsifying and biodegradability. It has various functions such as antibacterial activity, anti-ulcer activity, anti-cholesterol, intestinal useful bacteria growth promotion, anti-tumor activity, plant cell activation and immunoreactivity. It has been found to be a biological resource that can be applied to various fields such as health-oriented foods, medicines, food preservatives, heavy metal adsorbents, enzyme fixatives, cosmetics, feed and soil improving agents.

Chitin is not only resistant to chemicals, but also insoluble in water and most common solvents because acetylamino in the molecule is very strongly bound to hydrogen bonds between molecules.

On the other hand, chitosan is soluble in organic acids, such as low-density inorganic acid or acetic acid, maleic acid, but does not dissolve in water or alcohol, as well as the protein is present or there is a property that is cohesive when pH rises with the astringent taste, increase the viscosity There are many restrictions on its use.

Therefore, there are many problems to be solved in order to apply chitin and chitosan to a wide range of industries such as food and pharmaceutical fields.

In view of this, many studies have been conducted on chitin and chitosan derivatives having high physiological functionality, water solubility, solubility in organic solvents, and high stability. Among them, in particular, hydrolyzates including chitin and chitosan oligosaccharides are of interest. These concentrations of chitin and chitosan oligosaccharides have various functional characteristics such as antibacterial, antitumor activity, bifidus growth factor and plant cell activation, as well as rapid absorption in the body, which is expected to be applied as a high value-added material. .

Known methods for producing chitin and chitosan oligosaccharides have been developed by chemical degradation using strong acids and biological degradation using chitin and chitosan degrading enzymes.

The chemical decomposition of the former is not only a question of safety due to the use of strong acids, but also causes marine environmental pollution, removal of excess salts that occur during neutralization, low yield of higher oligosaccharides, and chemical reactions such as deamination and coloring during hydrolysis. Accompanied by the enzymatic degradation method, the latter enzymatic degradation method is safer than the chemical degradation method, and all chitosan hydrolysates currently on the market are manufactured by this method, but this method has a high market price of the enzyme. In the case of mass production, there is a problem that industrial production is still difficult because of the large economic burden such as expansion of biological process facilities.

Therefore, there is a need for the development of new decomposition methods that can compensate for the shortcomings of chemical and enzymatic degradation methods.

The present invention has been devised after a long study based on such necessity, and in order to develop a new decomposition process that is economical and environmentally friendly when chitosan is decomposed, chitosan is added to low-concentration organic acid and then decomposed by microwave heat treatment. Chitosan hydrolyzate was prepared in almost the same yield as that produced by the decomposition method, which will be described in detail as follows.

Chitosan is dissolved in a low concentration of 0.5% organic acid such as succinic acid, lactic acid and maleic acid at a concentration of 1 ~ 2%, and it is microwaved and it generates 121 ℃ by microwave digestion system (USA). This is a method for producing a functional chitosan hydrolyzate by decomposition for 200 minutes in.

Where% represents the g of the solute dissolved in 100 ml of the solution, and the chitosan concentration of 1 to 2% refers to the% concentration of the organic acid prepared at 0.5%.

The present invention thus made will be described in detail based on the following examples.

Example

1. Materials and Methods

(Production of chitosan)

The red snow crab is crushed to about 35mesh, 15 times of 2N HC1 is added thereto, and then demineralized at room temperature ( 25 ° C. ). Then, the protein is removed by reaction with 1N NaOH solution at 75 ° C. for 1 hour, filtered and washed with water. Decolorized with 0.4% sodium hypochlorite for 10 minutes, re-filtered and washed with water, and dried at 60 ° C with a hot air dryer to add 15 times 45% NaOH solution to the weight of chitin, followed by reaction at 100 ° C for 6 hours. It was prepared by drying at 60 ℃ through.

(Measurement of viscosity and deacetylation degree)

Viscosity was measured using a rotational viscometer (Brookfield LVTDV-II) by changing the rotational speed from 0.3rpm to 100prm at 25 ° C. At this time, the sample for viscosity measurement was dissolved in 0.5% acetic aicd solution according to Austin et al. 0.5% chitosan solution was used. On the other hand, the deacetylation degree of chitosan was measured by measuring the IR spectrum with an IR spectrophotometer (Shimadzu IR-408, Japan) by making a KBr cell and measuring the absorbance ratio (A1550 / A2878) at 1550 cm-1 to the absorbance at 2878 cm-1. From this ratio, the degree of deacetylation was determined using the calibration curve presented by Sannan et al.

(Production of chitosan hydrolyzate)

Chitosan was added to low concentration hydrochloric acid and organic acid, and hydrolyzate was prepared using a microwave digestion system (MDS 2000, CEM Co ,, USA).

(Yield of Chitosan Hydrolyzate)

The yield of the hydrolyzate was expressed as the sugar content per g of chitosan, and the sugar content was neutralized (pH 8.0) of the decomposed chitosan, centrifuged (12,000 rpm , 10 min), and then 1 ml of 5% phenol solution and 5 ml of concentrated sulfuric acid in 1 ml of the supernatant. After the addition of the absorbance at 470nm was measured from the standard calibration curve.

(Functional characteristic measurement)

(1) Antibacterial measurement

Antimicrobial activity of chitosan hydrolyzate was paper disk method. That is, Mueller Hinton agar medium was poured into a sterile petri dish by about 20m1 to make a plate, and the test strains were inoculated well first on a petri dish using a sterile swab incubated for 12 to 24 hours at 35 ° C.

Then, sterilized peter disk (8mm diameter, thick, Advantec Toyo Co., Japan) was put on the Mueller Hinton agar medium, and then a certain amount of the sample filtered through a membrane filter (0.45㎛) was injected and then a low temperature incubator (controlled at 37 ° C.) was used. The presence of antimicrobial activity was determined by the clear zone diameter (mm) around the paper disk after 24 hours and 48 hours incubation at Tokyo Science Works, Korea. The strain used in the antimicrobial experiment was Gram-positive bacteria. Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538. Gram-negative bacteria Escherichia coli ATCC 1129 and Enterobacter aerogenes ATCC 13048 was used. On the other hand, the minimum growth inhibition concentration was measured as follows according to Lorian's method. 0.1 ml of diluted solution was added to 9.8 ml of Mueller Hinter broth, brain heart infusion and YM broth, and then inoculated with 0.1 ml of various strains cultured for 18 to 24 hours and incubated at 35 ° C for 48 hours. After the growth of bacteria was measured by absorbance at 660nm to calculate the minimum growth inhibitory concentration required for growth inhibition.

(2) anti-cavity measurement

Anti-cavity was tested in the same way as the antimicrobial measurement method, the cavities used was Streptococcus intermedius .

(3) antihypertensive measurement

Antihypertensive properties were obtained by adding 50 µl of Hip-His-Leu (2.5 mM in borate buffer containing 200 mM NaCl, pH 8.3) to 25 µl of the sample solution according to the colorimetric measurement method using Trinitrobenzene Sulfonate (TNBS), and then diluting 5 times with ACE crude. 50 μl of enzyme solution was added and reacted at 37 ° C. for 1 hour.

250 μl of 0.5 M HCI was added as a stopping reagent, 250 μl of Kolthoff buffer (0.1 M Na2HPO4: 1.0N NaOH = 1: 2) was added thereto, followed by 25 μl of TNBS solution, followed by reaction for 20 minutes. After adding sulfite (4mM Na2SO3 in 0.2M NaH2PO4) to the absorbance at 416nm with a spectrophotometer (Shimadzu UV 140-02, Japan), the ACE inhibitory effect was calculated as a percentage before and after hydrolyzate addition.

Analysis of Oligosaccharide Composition of Chitosan Hydrolysate

The oligosaccharide composition of the chitosan hydrolyzate was confirmed by molecular weight distribution using a gel permeation chromatography analysis system (JASCO, Model LCSS-905, Jasco Co., Japan). That is, chitosan hydrolyzate was injected into the Shodex OHpak SB-801 + SB-803 column (7.5mm ID × 300mmL ), and the solution was extracted with a mobile phase (0.1M NaCl in 0.2% acetic acid) and detected by RI detector. Chitosan oligosaccharides and pullulan (MW 853000, 95400, 23700, 5800) from 1-6 sugars were purchased from Wako, Japan for the analysis of oligosaccharide composition.

(Preparation of Hydrolyzate-Containing Leaded Product and Quality Measurement

Chitosan hydrolyzate was added to 0.5%, 1.0%, 1.5%, and 2.0% of frozen pollack paste and heated at 90 ° C to prepare a soft product. It was measured by incubation at 20 ℃ by the medium method, browning degree was measured using a direct color difference meter (Model ND-1001DP, Denshoku kogyo Co., Japan). In addition, the strength and hardness of the jelly was measured by a rheometer (Compac-100, Sun scientific Co., Japan) attached to a spherical plunger with a diameter of 10mm after cutting the soft product into 1cm thickness.

2. Results and Discussion

Yield of chitosan hydrolyzate prepared by physicochemical decomposition

(1) Hydrolysis of Chitosan by Addition of Low Concentration Organic Acid

Recently, research results of dissolving chitosan in low concentration hydrochloric acid and acetic acid when hydrolyzing chitosan for the purpose of preparing chitosan oligosaccharide and then hydrolyzing by ultrasonication have been reported, suggesting the possibility of the practical use of physicochemical decomposition using a decomposition device.

In this study, chitosan was dissolved in low-concentration organic acid, which is harmless to human body, and then produced chitosan hydrolyzate by physicochemical decomposition using microwave heating device. Also , various factors affecting yield were examined.

1) Yield of hydrolyzate by microwave heating and high temperature and high pressure treatment

Chitosan (deacetylation: 92%, viscosity: 126cP) prepared from red crab chitin was dissolved in 0.5% succinic acid at 2% concentration, and then dissolved at the same temperature using a two-value decomposition device, that is, microwave heating and high temperature and high pressure equipment. After digestion at 200 minutes at 121 ° C. for 50 minutes, the yield is expressed as a sugar content.

On the other hand, chitosan addition concentration of 2% was determined through a preliminary experiment, when more than 3% was insoluble in 0.5% organic acid.

At the same time, the content of sugars of chitosan hydrolyzate decomposed by microwave heating was higher than that of chitosan hydrolyzate decomposed by high temperature and high pressure.

-▲-: Microwaving treatment,-●-: Autoclavin treatment

In other words, the total sugar content of the hydrolyzate decomposed by microwave heating for 100 minutes and 200 minutes was 408 mg and 565 mg per g of chitosan, respectively, and the content of the total sugar content of the samples decomposed for 100 minutes and 200 minutes at high temperature and high pressure was 368 mg and 532 mg, respectively. Because of the high content of decomposed hydrolyzate, the microwave heating device was an effective way to increase the yield of chitosan hydrolyzate.

2) Yield of hydrolyzate according to chitosan molecular weight difference

H ₂ O ₂ was added to the high store chitosan (126cP), reacted at 40 ° C., washed with ethanol, and lyophilized to obtain a medium viscosity (98.5 cP) and low viscosity (7.4 cP) chitosan. Three different chitosans with different molecular weights were dissolved in 0.5% succinic acid at a concentration of 2%, and then digested by microwave heating at 121 ° C. for 100 minutes.

Under the same reaction conditions, the total sugar content of the hydrolyzate prepared with low viscosity chitosan (7.4 cP) was 585 mg per g of chitosan (98.5 cP) and the total sugar content of the high viscosity (126 cP) chitosan hydrolyzate (medium viscosity: 489 mg, high viscosity: 400 mg). That is, when chitosan having a small molecular weight was used, a high yield of chitosan hydrolyzate was produced in the same time period.

3) Yield of hydrolyzate according to organic acid type

Low viscosity chitosan (7.4 cP) was dissolved in 0.5% of succinic acid, lactic acid and maleic acid at 2% concentration, and then hydrolyzed for 200 minutes at 121 ℃ for 50 minutes by microwave heating. Indicated.

Regardless of the type of organic acid, the sugar content increased as the decomposition time increased. Among the three organic acids, the content of the sugar content of chitosan hydrolysate dissolved by 0.5% succinic acid and decomposed by microwave heating was the highest. At 585 mg and 715 mg per g of chitosan, respectively.

-●-: 0.5% Succinic acid,

-▲-: 0.5% Lactic acid,

-■-: 0.5% Maleic acid,

On the other hand, the total sugar content of chitosan hydrolyzate dissolved in lactic acid and decomposed under the same conditions was 430mg and 645mg, respectively, and the content of sugar content of chitosan hydrolyzate dissolved in maleic acid was 380mg and 565mg, respectively. Therefore, among the organic acids, succinic acid was the most suitable solvent to increase the yield in the preparation using microwave heating of chitosan hydrolyzate.

4) Yield of hydrolyzate according to chitosan concentration

The yield of the hydrolyzate was thought to be different depending on the concentration of chitosan added to the organic acid , so that 0.5%, 1.0%, 1.5% and 2.0% of low viscosity chitosan (7.4cP) was added to 0.5% succinic acid , followed by 100 at 121 ° C. The relative yield was measured when the yield of 0.5% chitosan was added to 100 after hydrolysis by microwave heating for 100 minutes.

The relative yield of the 1.0% chitosan added group was 95.2%, the 1.5% and 2.0% chitosan added groups were 75.5% and 65.6%, respectively, and the yield was higher at 1.0% or less chitosan added group, and the yield decreased as the amount of chitosan added. On the other hand, numerically, the yield of decomposition was the highest in the 0.5% chitosan addition group, but the 1.0% chitosan addition condition was most suitable in consideration of economic aspects because of the disadvantage that the amount of organic acid addition was consumed relatively high.

In summary, in order to increase the yield of hydrolyzate during hydrolysis of chitosan using microwave heating , microwave heating was more effective than high temperature and high pressure equipment, and the smaller the molecular weight of the raw material chitosan, the higher the yield. On the other hand, 0.5% Succinic acid was the organic acid that can increase the yield of the hydrolyzate as a solvent, chitosan addition concentration was suitable 1.0%.

(2) Hydrolysis of chitosan by adding low concentration hydrochloric acid

The yield of chitosan hydrolyzate was investigated when the hydrolysis of chitosan was decomposed by microwave heating with the addition of low concentration hydrochloric acid.

After adding 1% low-viscosity chitosan (7.4cP) to 1-3M hydrochloric acid and decomposing it at 121 ° C by microwave heating, the yield of chitosan hydrolyzate increased with increasing hydrochloric acid concentration. The starch content of the hydrolyzate was 870mg per g of chitosan, but it was dissolved in 0.5% succinic acid under 90% hydrolysis under the same conditions, but it was dissolved in 0.5% succinic acid under the same conditions. Slightly less than 890 mg).

-○-: Control (5% succinic acid),

-●-: 1M HCL,-▲-: 2M HC1

-■-: 3M HC1

In addition, in order to compare the yield of chitosan hydrolyzate prepared by the acid hydrolysis method and the yield of chitosan hydrolyzate prepared by adding low concentration hydrochloric acid and organic acid, low viscosity chitosan (7.4) in high concentration (12M, 10M and 8M) hydrochloric acid was compared. After adding cP), the yield of chitosan hydrolyzate decomposed in a 70 ° C. constant temperature water bath was shown.

-●-: 8M HC1,-▲-: 10M HCl,

-■-: 12M HCl

Among the chitosan hydrolysates prepared by the acid hydrolysis method, the yield of the highest yield per solvent, that is, the total sugar content per 1 g of chitosan obtained by hydrolysis of 12M hydrochloric acid for 90 minutes was 860 mg, using 0.5% organic acid and 3M hydrochloric acid and the same time at 121 ° C. It was almost the same as the sugar content (870-890 mg) of the physicochemically degraded sample.

Therefore, the physicochemical method of dissolving in low concentration organic acid and then decomposing by microwave heating was not much different from the acid hydrolysis method in which only hydrochloric acid was decomposed in terms of the yield of hydrolyzate, and in particular, complex purification such as neutralization or desalting operation after hydrolysis. It is recognized as an economical decomposition method that does not require a process, and may be usefully applied to hydrolysis of chitosan in the future.

Functional Properties of Chitosan Hydrolysates

(1) antibacterial and anti-cavity activity

Table 1 shows the antimicrobial activity of gram-negative bacteria of chitin and chitosan hydrolysates by paper disk method.

The antimicrobial effect of Escherichia coli , which is the subject of bacteriological examination of food, was all antimicrobial activity in 6 chitosan hydrolysates (CHM-1 to CHA-3) decomposed at 121 ° C by microwave heating and high temperature and high pressure. In chitin hydrolyzate, hydrolyzate decomposed at 121 ° C for 50 minutes by microwave heating.

(CM-2, starch content: 613 mg / g chitin) and hydrolyzate decomposed for 90 minutes (CM-3, starch content: 830 mg / g chitin), and hydrolyzate decomposed for 90 minutes at 121 ° C. at high temperature and high pressure (CA- 3, starch content: 740mg / g chitin) Esherichia coli There was an antimicrobial effect against.

Antimicrobial activity against Enterobacter aerogenes , a pathogenic enterobacteriaceae, was not found in chitin hydrolyzate. Among chitosan hydrolysates, hydrolysates (CHM-2, starch content: 870mg / g chitosan) and hydrolysates digested for 200 minutes by microwave heating It showed excellent antimicrobial activity in the digested products (CHA-3, starch content: 940mg / g chitosan) and hydrolysates (CHA-3, starch content: 880 mg / g chitosan) which were decomposed at high temperature and high pressure for 200 minutes.

On the other hand, the antimicrobial effects of the chitin and chitosan hydrolysates on Gram-positive bacteria are shown in Table 2 .

The antimicrobial effect against Bacillus cereus was excellent in chitosan hydrolysates, especially in CHM-2 and CHM-3. Chitin hydrolysates had antibacterial activity in CM-2 and CA-3, but not CM-3.

Antimicrobial effect against Bacillus subtilis was excellent in chitosan hydrolysates CHM-3 and CHA-3. On the other hand, chitin hydrolyzate had lower antimicrobial activity than chitosan hydrolyzate, but had antimicrobial activity in CA-3 and CM-3.

The antimicrobial effect against Staphylococcus aureus , a pathogenic food poisoning bacterium, was in CM-2 and CM-3 among chitin hydrolysates, and chitosan hydrolysates had antimicrobial activity.

On the other hand, the anti-cavities activity of the chitin and chitosan hydrolysates against the caries Streptococcus intermedius is shown in Table 3 .

Anti-cavity activity was seen in chitin and chitosan hydrolysates, and formed the largest clear zone in CHM-3.

In addition, low-viscosity chitosan (7.4cP) used as a control had antimicrobial effect only against the anti-caries bacillus Streptococcus intermedius , no antimicrobial effect against the rest of the bacteria, 0.5% succinic acid antibacterial against all bacteria when present alone There was no activity at all.

In conclusion, chitosan hydrolyzate showed better antibacterial effect than chitin hydrolyzate. Especially, hydrolyzate (CHM-3) decomposed for 200 minutes at 121 ℃ by microwave heating was superior to all 6 strains. It showed antimicrobial effect.

(2) antihypertensive

The antihypertensive activity (ACE inhibitory effect) was tested to determine whether the chitosan hydrolyzate can inhibit hypertension, which is an adult disease. On the other hand, the low viscosity, medium viscosity, and high viscosity chitosan tested as a control showed some inhibitory effects, and the higher the molecular weight of chitosan, the better the ACE inhibitory effect.

Changes in Quality of Chitosan Hydrolyzate Added Soft Products during Low Temperature Storage

Chitosan is a natural polymer with antimicrobial activity and has been reported to extend the shelf life of food by adding it to kimchi and tofu because of its availability as a food preservative.

Chitosan hydrolyzate prepared by microwave heating after addition of low concentration organic acid not only showed superior antimicrobial activity than polymer chitosan, but unlike acid hydrolyzate, it can be directly applied to food without undergoing neutralization and desalting process. It is expected to be used as a natural food preservative in the future.

Accordingly, hydrolyzate of chitosan hydrolyzate was added to 0.5% succinic acid with low viscosity chitosan (7.4 cP) in 1% concentration for 200 minutes at 121 ° C by microwave heating. (FA grade) The soft products prepared by adding 0.5%, 1.0%, 1.5% and 2.0% by weight were stored at 5 ° C. at low temperature, and the changes in viable cell number, browning degree, jelly strength and hardness were examined.

(1) Change of viable cell number and browning degree

The change of viable cell number and browning degree during storage at 5 ° C of soft products prepared by the addition of chitosan hydrolyzate was shown.

-■-: control,-◆-: 0.5%-■-: control,-◆-: 0.5%

-●-: 1.0%,-▲-: 1.5%-●-: 1.0%,-▲-: 1.5%

-※-: 2.0%-※-: 2.0%

Chitosan hydrolyzate added products had fewer viable cell counts than unadded leaded products regardless of the amount added up to 18 days of storage. In particular, the soft product prepared by adding 2% chitosan hydrolyzate showed a good antimicrobial effect as the number of viable cells decreased rather than the 6th day of storage. On the other hand, browning degree was almost no difference between the chitosan hydrolyzate added group and no added group, and there was no browning inhibitory effect.

(2) change of jelly strength and hardness

In order to investigate the texture change of soft products by the addition of chitosan hydrolyzate, the change of jelly strength and hardness during low temperature storage was shown.

-■-: control,-◆-: 0.5%-■-: control,-◆-: 0.5%

-●-: 1.0%,-▲-: 1.5%-●-: 1.0%,-▲-: 1.5%

-※-: 2.0%-※-: 2.0%

Jelly strength decreased during storage for all products, and the decrease was not significantly correlated between the products. Regardless of the storage days, the jelly strength of chitosan hydrolyzate-added soft products was about 2,000 to 3,000 g · cm , which was significantly higher than the jelly strength of unadded soft products (about 1,700 to 1,800 g · cm ). It is thought to be due to viscosity.

On the other hand, according to the addition amount of chitosan hydrolyzate, the jelly strength was different, showing the highest jelly strength in the 0.5ml addition group, and the higher the addition amount, the lower the jelly strength. In addition, the hardness of chitosan hydrolyzate added group showed higher value than that of the non-added group. Jang et al. Reported that breaking strength was increased when enzyme-decomposed chitosan hydrolyzate was added to fish meat products.

In conclusion, the chitosan hydrolyzate decomposed in combination with low concentration of organic acid and microwave heating was concluded that the addition of the product in the production of soft products can not only enhance the texture but also inhibit the growth of bacteria and extend the shelf life.

3. Conclusion

The dissolved chitosan new hydrolysis methods that can compensate the shortcomings of the conventional chemical and enzymatic degradation method is stable chitosan to develop purpose to the low-concentration organic acid by using a microwave heating hydrolysis following factors on the yield of the hydrolyzate Was reviewed. In addition, the antimicrobial, anti tooth decay and antihypertensive properties of these hydrolyzates were tested and the hydrolyzate with excellent antimicrobial activity was added during the production of fish meat products.

(1) As a condition for increasing the yield when the chitosan was dissolved in low concentration organic acid and then decomposed by microwave heating, the smaller the molecular weight of the raw chitosan, the higher the yield. Among the organic acids, succinic acid showed the highest yield.

On the other hand, the yield of chitosan hydrolyzate dissolved in 0.5% succinic acid and decomposed at 121 ° C. by microwave heating was not significantly different from the yield of chitosan hydrolyzate decomposed at the same time using only 12M concentrated hydrochloric acid.

(2) Antimicrobial activity against chitosan hydrolyzate after microwave heating after dissolving in 0.5% succinic acid showed antibacterial activity in all sections, especially in the experimental group (CHM-3) hydrolyzed at 121 ° C for 200 minutes in microwave. The activity was the best.

(3) The chitosan hydrolyzate had anticariogenic activity in all sections, but antihypertensive synthesis was not found in all sections, and it was confirmed that there was some antihypertensive effect in the control polymer chitosan. Minimum inhibitory concentration on the strains of the CHM-3 has a molecular weight of 10,000 or less of the oligosaccharide fractions from Fraction 3 CHM-beams is low.

(4) The soft product prepared by adding CHM-3 had less viable cell number by 18 days of storage and higher jelly strength and hardness than the non-added product at 5 ℃ cold storage.

The chitosan decomposition method of the present invention is the first experimental result applied at home and abroad, the yield of chitosan hydrolyzate produced by this is almost the same as the yield of chitosan hydrolyzate prepared by the conventional chemical decomposition method is possible to practical use, especially in fish paste As a result of the addition and storage at low temperature, the amount of viable bacteria was very low compared to the non-added fish paste, and it was proved to be excellent in antimicrobial and anti-cavity activity, and the texture was excellent. Since the process is not necessary, the manufacturing cost of the chitosan hydrolyzate can be greatly lowered, and thus the effect is very high, such as being applicable to all fields such as natural preservatives of food and raw materials of functional livestock feed.

Claims (1)

A heating device capable of dissolving organic acids, such as succinic acid, lactic acid, and maleic acid, by adding 0.5 g to 100 ml of water, and adding 1-2 g of chitosan to the solution and dissolving it. microwave digestion system, USA) for the production of functional chitosan hydrolyzate using microwave which is decomposed at 121 ° C. for 200 minutes.