KR100291572B1 - Process for producing liquid sterilizing agent - Google Patents

Process for producing liquid sterilizing agent Download PDF

Info

Publication number
KR100291572B1
KR100291572B1 KR1019970082320A KR19970082320A KR100291572B1 KR 100291572 B1 KR100291572 B1 KR 100291572B1 KR 1019970082320 A KR1019970082320 A KR 1019970082320A KR 19970082320 A KR19970082320 A KR 19970082320A KR 100291572 B1 KR100291572 B1 KR 100291572B1
Authority
KR
South Korea
Prior art keywords
container
solution
sterilizing agent
present
water
Prior art date
Application number
KR1019970082320A
Other languages
Korean (ko)
Other versions
KR19990062015A (en
Inventor
조영태
Original Assignee
조영태
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 조영태 filed Critical 조영태
Priority to KR1019970082320A priority Critical patent/KR100291572B1/en
Publication of KR19990062015A publication Critical patent/KR19990062015A/en
Application granted granted Critical
Publication of KR100291572B1 publication Critical patent/KR100291572B1/en

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/78Saururaceae (Lizard's-tail family)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/77Sapindaceae (Soapberry family), e.g. lychee or soapberry
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/82Theaceae (Tea family), e.g. camellia
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Landscapes

  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

PURPOSE: Provided is a process for producing a liquid sterilizing agent which has a strong sterilizing activity regardless exposure to sun light for a long time or long-term storage. CONSTITUTION: The process for producing a liquid sterilizing agent comprises the steps of: dissolving 1 kg of sugar in 1000 cc of water in a vessel; adding 50 to 100g of edible plants as a solute into the sugar solution; sealing the vessel with a piece of air-penetrating cloth; fermenting the solution at 25 to 30 deg.C for 15 to 20 days until the hydrogen concentration of the solution become below pH 2.7; and filtrating the fermented solution and removing wastes to produce a liquid sterilizing agent, in which the edible plants include leaf and stalk of Houttuyniae Herba, and citron fruits.

Description

살균액 제조방법Sterilization Solution

본 발명은 살균액 제조방법에 관한 것으로서, 더욱 상세하게는 당이 용해된 물에 식물 수확물을 첨가한 후, 소정상태에서 발효시켜 수소 이온지수 2.7이하의 초살균작용을 갖는 강산성액을 얻을 수 있는 살균액 제조방법에 관한 것이다.The present invention relates to a method for producing a sterilizing liquid, and more particularly, after adding a plant harvest to water in which sugar is dissolved, it is fermented in a predetermined state to obtain a strong acidic liquid having a super sterilization effect of 2.7 or less hydrogen ion index. It relates to a sterilizing liquid production method.

일반적으로 강산성수는 그 높은 산화환원 전위수 때문에 세균과 접촉하면 수초 뒤 세균에서 전자를 빼앗아서 산화 살균하고, 강산성 전해 생성수 자신은 전자를 얻어서 중화되어서 연한 식염수가 된다.In general, strong acidic water is oxidized and sterilized by taking electrons from bacteria after a few seconds when they come into contact with bacteria because of their high redox potential water, and the strong acid electrolytically generated water itself is neutralized to become soft saline.

강산성수는 세균, 바이러스, 진균에 살균적 효과가 있고, 상처, 난치성 피부 궤양에 효과적인 것으로 알려져 있다.Strong acidic water has a bactericidal effect on bacteria, viruses and fungi, and is known to be effective in wound and refractory skin ulcers.

또한, 강산성수의 살균작용은 산화, 환원 전위가 가진 물리적인 작용에 의한 것으로, 화학적인 작용에 의한 화학약품과는 본질적으로 상이한 것으로 판명되었다.In addition, the sterilization action of the strong acidic water is due to the physical action of the oxidation and reduction potential, and has been found to be essentially different from the chemical action by the chemical action.

최근에는 옅은 소금물을 전기분해하여 강산성수를 얻은 방법이 제안된 바가 있다.Recently, a method of obtaining strong acidic water by electrolyzing light brine has been proposed.

도 1 은 종래 강산성수 제조장치를 보이는 사시도이다.1 is a perspective view showing a conventional strong acid water production apparatus.

도 1 에 도시된 바와 같이 옅은 소금물이 담긴 통(10)을 특수용 격막(12)으로 양분하고, 양극(14)과 음극(16)을 각각 양쪽에 꽂아 전기분해를 하면, 양극(14)이 들어간 쪽에 강력한 산성수가 발생되도록 구성된다.As shown in FIG. 1, the pail 10 containing the light salt water is bisected into the special diaphragm 12, and the positive electrode 14 and the negative electrode 16 are plugged into both sides to be electrolyzed. It is configured to generate strong acidic water on the side.

그러나, 전술한 바와 같이 구성된 종래 강산성수 제조장치에서 제조된 강산성수는 소정시간이 흐름에 따라 살균력이 저하되는 문제점이 있다.However, the strong acidic water produced in the conventional strong acidic water production apparatus configured as described above has a problem that the sterilizing power is lowered as the predetermined time passes.

또한, 햇빛에 약해서 햇빛에 장시간 노출될 경우 살균력이 현저하게 저하되는 문제점이 발생된다.In addition, the weakness of sunlight causes long-term exposure to sunlight causes a problem that the sterilization power is significantly reduced.

본 발명은 전술한 문제점을 해결하기 위해 이루어진 것으로서, 당이 용해된 물에 식물 수확물을 첨가한 후, 소정상태에서 발효시켜 수소 이온지수 2.7이하의 초살균작용을 갖는 강산성액을 얻을 수 있는 살균액 제조방법을 제공함에 그 목적이 있다.The present invention has been made to solve the above-described problems, sterilization liquid which can obtain a strong acidic liquid having a super sterilization action of hydrogen ion index of 2.7 or less by adding a plant harvest to water in which sugar is dissolved and then fermenting in a predetermined state. The purpose is to provide a manufacturing method.

전술한 목적을 달성하기 위해 본 발명은 다음과 같이 구성된다. 즉, 본 발명은 물 1000cc 정도 담긴 용기 내에 당 1kg 정도를 용해하는 단계; 용기 내에 식물 수확물을 50~100g을 투입하는 단계; 용기를 통기성 천으로 봉한 후, 25~30℃의 온도조건하에서 15~20일 동안 보관 발효시켜 용기 내의 수소 이온지수(pH)를 2.7 이하로 하는 단계 및 용기에서 찌꺼기를 걸러내어 액만을 얻는 단계로 구성된 특징이 있다.In order to achieve the above object, the present invention is configured as follows. That is, the present invention comprises the steps of dissolving about 1kg per container in a container containing about 1000cc of water; Injecting 50-100 g of plant harvest into the container; After sealing the container with a breathable cloth, storing and fermenting for 15-20 days under the temperature condition of 25 ~ 30 ℃ to lower the hydrogen ion index (pH) in the container to 2.7 or less to filter out the residue from the container to obtain a liquid only It is composed of features.

제1도는 종래 강산성수 제조장치를 보이는 사시도.1 is a perspective view showing a conventional strong acid water production apparatus.

제2도는 본 발명의 살균액 제조용기를 보이는 사시도.Figure 2 is a perspective view showing a sterilizing liquid production container of the present invention.

제3도는 본 발명의 살균액 제조방법을 보이는 순서도.Figure 3 is a flow chart showing a sterilizing liquid production method of the present invention.

〈도면의 주요부분에 대한 부호의 설명〉<Explanation of symbols for main parts of drawing>

100 : 용기 110 : 식물 수확물100 container 110 plant harvest

120 : 통기성 천120: breathable cloth

이하에서는 첨부 도면을 참조하여 본 발명에 따른 살균액 제조방법의 바람직한 실시예를 상세히 설명하기로 한다.Hereinafter, with reference to the accompanying drawings will be described in detail a preferred embodiment of the sterilizing solution production method according to the present invention.

도 2 는 본 발명의 살균액 제조용기를 보이는 사시도이고, 도 3 은 본 발명의 살균액 제조방법을 보이는 순서도이다.Figure 2 is a perspective view showing a sterilizing liquid production container of the present invention, Figure 3 is a flow chart showing a sterilizing liquid production method of the present invention.

도 2 에 도시된 바와 같이 본 발명에 따른 살균액의 제조는 먼저, 물(H2O)에 당(예를 들면, 설탕, 포도당 및 과당)을 용해시킨 후, 용질인 식용 식물(한 종류 또는 두 종류 이상을 선택하여 사용함; 110)을 투입하여 용기(100)에 이물질이 들어가지 않고 공기의 유입이 가능하도록 통기성 천(120)으로 용기(100)를 덮고, 25~30℃의 온도조건하에서 15~20일 동안 발효시키게 된다.As shown in FIG. 2, the sterilizing solution according to the present invention is first dissolved in sugar (for example, sugar, glucose and fructose) in water (H 2 O), and then edible plant (one type or solute). Selecting two or more types; using 110 to cover the container 100 with a breathable cloth 120 to enable the inflow of air without entering foreign matter into the container 100, under the temperature conditions of 25 ~ 30 ℃ It will ferment for 15-20 days.

이때, 사용되는 물은 살균된 것을 사용하는 것이 바람직하며, 용질인 식용식물로는 어성초의 잎, 어성초의 줄기, 유자의 열매, 설련화, 녹차 등을 각각 또는 상호 조합하여 투입한다. 한편, 전술한 바와 같은 식용식물 이외에 흑임자, 생강, 다시마, 인삼, 사과, 귤, 오이, 콩, 녹차, 마늘, 감초, 차전초, 주목, 은행, 동충하초, 옥수수, 당근, 포공영 등의 채소, 과일 및 약초 등을 사용할 수 있으며, 이외에도 식용할 수 있는 식물이면 어느 것이든 가능함은 당연하다. 그리고, 용기(100) 내에 투입되는 용질로써의 식용식물(110)의 양은 50~100g 정도를 투입한다.At this time, it is preferable to use the sterilized water, and the soy edible plant is added to each of the leaves or the stem of the fish, stems of citron, citron fruit, sulyeonhwa, green tea and the like, or in combination with each other. On the other hand, in addition to the above-mentioned food plants, black sesame seeds, ginger, kelp, ginseng, apples, tangerines, cucumbers, beans, green tea, garlic, licorice, chajeoncho, yew, ginkgo, cordyceps, corn, carrots, poongyoung and other vegetables, fruits and Herbal medicine and the like can be used, and any other edible plant is natural. Then, the amount of the edible plant 110 as the solute introduced into the container 100 is put into about 50 ~ 100g.

전술한 바와 같이 25~30℃의 온도조건하에서 15~20일 정도 발효시키는 동안 공기와 용액이 결합하여 산화가 촉진되며, 용질인 식용식물의 영양소(탄소화물, 수분, 단백질, 지방, 기타 미량원소)가 효소 분해 및 이온화 작용에 의해 물질의 가수분해와 결합과정을 서서히 거치게 된다. 한편, 식용식물의 식물의 섬유질은 찌꺼기로 가라앉으며, 용액이 15~20일 정도의 과정을 거쳐 pH 2.7 이하의 다수의 아미노산이 결합된 폴리펩타이드 류의 단백질 용액이 생성된다.As mentioned above, air and solution are combined to promote oxidation during fermentation for about 15 to 20 days under the temperature condition of 25 ~ 30 ℃, and nutrients (carbohydrates, moisture, protein, fat, other trace elements) of edible plant are solute. The enzymatic digestion and ionization process causes the material to hydrolyze and bind slowly. On the other hand, the fiber of the edible plant sinks to the debris, the solution is a protein solution of a polypeptide-like polypeptide that combines a plurality of amino acids of pH 2.7 or less through a process of about 15 to 20 days.

한편, 본 발명에 따른 살균액은 각기 다른 종류의 식용식물로 제조 후 2종 이상 배합 사용할 수 있으며, 식물의 주요 성분이 다소 유무의 차이는 있으나, 수분, 단백질, 지방, 탄수화물, 기타 미량원소이고 그 구성 원소도 탄소:C, 수소:H, 산소:O, 질소:N, 미량원소로 공통된다.On the other hand, the sterilizing solution according to the present invention can be used after mixing two or more kinds of food plants of different kinds, and the main components of the plant is somewhat different, whether there is water, protein, fat, carbohydrates, and other trace elements The constituent elements are also common to carbon: C, hydrogen: H, oxygen: O, nitrogen: N, and trace elements.

식용식물 한 종류씩 본 발명에 따른 제조방법에 의해 제조된 살균액은 각 식물마다 pH치가 조금씩 차이가 있을 수 있으나 2종류 이상의 식용식물을 배합할 경우는 본 발명에서 요구하는 pH치를 2.7이하 pH 0까지 제조할 수 있으며, pH 0 이하 상태가 되면 산화 환원 전위(Eh)에 관계없이 유해 미생물이 생존할 수 없는 환경이 조성된다.The sterilizing solution prepared by the manufacturing method according to the present invention by each type of edible plant may have a slightly different pH value for each plant, but when two or more types of edible plants are blended, the pH value required by the present invention is less than or equal to pH 2.7 or less. It can be manufactured up to, and when the pH is below 0, an environment in which harmful microorganisms cannot survive regardless of the redox potential (Eh) is created.

한편, 여러 가지 물질들의 pH를 보면 위액은 pH 1~3, 레몬은 pH 2.2~2.4, 식초는 pH 2.4~3.4, 포도주는 pH 2.8~3.8, 사과는 pH 2.9~3.3, 콜라는 pH 2.7~2.8, 아스파라긴산은 pH 3 등으로 본 발명에 의해 제조된 살균액과 비교하여 별 차이가 없음을 알 수 있어 본 발명에 의해 제조된 살균액 역시 강산성에 속하지만 천연 단백질 류의 유기산 용액으로 독성이 없다.On the other hand, the pH of various substances is pH 1 ~ 3, pH 2.2 ~ 2.4 for lemon, pH 2.4 ~ 3.4 for vinegar, pH 2.8 ~ 3.8 for wine, pH 2.9 ~ 3.3 for apple, pH 2.7 ~ 2.8 for coke Aspartic acid, pH 3, etc., it can be seen that there is no difference compared to the sterilizing solution prepared by the present invention, the sterilizing solution prepared by the present invention also belongs to strong acidity, but is not toxic as an organic acid solution of natural protein.

전술한 바와 같은 식물군 중 어성초는 그 성분 중 데카노일 아세트알데히드라는 성분이 강한 생명력을 가지고 있으며, 녹차의 카테킨이란 성분은 헬리코 박터 파이로리균 살균작용이 있으며, 당근에 함유된 당질 코르티코이드는 항염증 작용과 궤양성 대장염, 아토피성 피부염의 개선에 효과가 있는 것으로 알려져 있다.Among the plant groups described above, the fish vinegar has a strong vitality such as decanoyl acetaldehyde, and the catechin component of green tea has bactericidal action of Helicobacter pylori, and the carbohydrate corticoids contained in carrots are anti-inflammatory It is known to be effective in improving the action, ulcerative colitis and atopic dermatitis.

컴프리 차에는 유기 게르마늄이 풍부하며 게르마늄은 치조농류와 같은 포도상구균에 의한 모든 병에 탁월한 살균 효과가 있는 것으로 알려져 있으며, 초피나무, 까마중, 한련초, 향나무, 감초, 마늘, 겨우살이, 포공영, 동충하초, 석창포, 주목, 질경이(차전초), 삼백초 등의 수많은 식물들이 살균 항균 성분을 가지고 있어 이러한 식물을 이용하여 제조된 본 발명의 살균액 역시 그 식물에 관련하는 효과를 나타낸다고 할 수 있다.Comfrey tea is rich in organic germanium and germanium is known to have excellent sterilizing effect on all diseases caused by staphylococcus aureus, such as alveolar, etc. Many plants, such as, yew, plantain (chajeoncho), three hundred candles, etc. have a bactericidal antimicrobial component, it can be said that the sterilizing solution of the present invention prepared using such a plant also has an effect related to the plant.

본 발명에 의해 제조된 살균액 중 어성초, 차잎, 인삼, 은행잎, 호도, 유자, 깨, 감나무잎, 감초, 산초, 밤나무를 배합하여 다수의 아미노산이 결합된 폴리펩타이드 단백질의 용액을 두피에 도포시 작용은 먼저, 캐라틴, 데카노일 아세트알데히드 등의 성분이 두피를 살균하여 청결히 하고, 쿠에르치트린이란 성분의 모세혈관 강화작용을 통해 국소 혈류량을 증가시켜 모세혈관을 통해 영양과 산소를 충분히 공급하는 작용을 하고, 당질 및 식물성 지방성분이 보습작용을 하며, 비타민, 미량원소, 아미노산, 핵산 등이 생성된 용액으로 모낭에 영양을 공급하기 때문에 이와 같은 작용으로 탈모를 방지할 수 있게 된다.In the sterilization solution prepared according to the present invention, when a solution of a polypeptide protein in which a plurality of amino acids are combined is applied to the scalp by combining fish leaf, tea leaf, ginseng, ginkgo biloba, hoe, citron, sesame, persimmon leaf, licorice, Japanese herb and chestnut First, carotene, decanoyl acetaldehyde, etc., sterilize the scalp and clean it, and increase the local blood flow through the capillary strengthening action of the ingredient called querchithrin to supply nutrition and oxygen through the capillaries. It acts as a moisturizing effect of sugars and vegetable fats, and vitamins, trace elements, amino acids, nucleic acids, etc. are produced to supply hair follicles to prevent hair loss by this action.

[실시예 1]Example 1

먼저, 살균한 물 1000cc 정도 담긴 다수의 용기(100) 내에 각각 설탕을 1kg 투입하여 정도를 용해시켰다.First, 1 kg of sugar was added to each of a plurality of containers 100 containing about 1000 cc of sterilized water to dissolve the degree.

각 용기(100) 내의 물에 설탕 1kg 정도씩을 용해시킨 각각의 용액에 용질로써 작용하는 식용식물(110)인 차전초, 마늘, 은행, 어성초, 콩, 인삼, 솔잎을 각각 100g씩 투입하였다.100 g of jeonchocho, garlic, ginkgo, ginseng, soybean, ginseng, pine needles, which are edible plants 110 acting as a solute, were added to each solution in which about 1 kg of sugar was dissolved in water in each container 100.

용기(100) 내에 용질로써의 식용식물을 투입한 후, 각각의 용기를 통기성 천(120)으로 봉하고, 각각의 용기를 25~30℃의 온도조건하에서 유지한 채로 20일 동안 발효시켜 본 발명에서 제조하려는 살균액을 제조하였다.After adding the edible plant as a solute in the container 100, each container is sealed with a breathable cloth 120, and each container is fermented for 20 days while being maintained at a temperature of 25 ~ 30 ℃ in the present invention The sterilizing solution to be prepared was prepared.

전술한 바와 같이 실시예1과 같이 제조된 살균액이 pH 2.7 이하로 형성되는 과정을 표 1에 나타내었다.As described above, the sterilizing solution prepared as in Example 1 is shown in Table 1 to form a pH of 2.7 or less.

Figure kpo00001
Figure kpo00001

표 1 에서와 같이 본 발명에 따른 살균액은 그 경과일이 지날수록 강산성화되어 20일 정도에는 pH 2.7 이하의 살균액이 생성됨을 알 수 있다.As shown in Table 1, the sterilizing solution according to the present invention is strongly acidified as the elapsed days pass, and the sterilizing solution having a pH of 2.7 or less is produced in about 20 days.

본 발명에 따른 제조방법에 의해 제조된 살균액을 한국화학연구소에 의뢰하여 MIC 테스트를 의뢰한 결과를 표 2 에 나타내었다. 표 2 에서 셈플명 A는 식물 차잎, 어성초, 측백나무잎을 혼합하여 제조한 살균액이고, 셈플명 B는 식물 차잎, 차전초, 솔잎, 마늘을 혼합하여 제조한 살균액이며, 셈플명 C는 본 출원인의 연구를 위해 세라믹 볼을 투입하여 제조한 살균액으로 본 발명과는 무관한 것이다.Table 2 shows the results of requesting the MIC test by requesting the sterilization solution prepared by the method according to the present invention to the Korea Research Institute of Chemical Technology. In Table 2, sample name A is a sterilizing solution prepared by mixing plant tea leaves, vinegar, and cypress leaves, and sample name B is a sterilizing solution prepared by mixing plant tea leaves, tea leaves, pine needles, and garlic. Sterilizing solution prepared by applying ceramic balls for the applicant's research is irrelevant to the present invention.

Figure kpo00002
Figure kpo00002

전술한 바와 같은 표 2 에서 0.05는 20배 희석용액을 의미하고, 0.10은 10배 희석용액을 의미한다. 표 2 에서와 같이 식물을 배합하여 제조된 A, B는 병원균 1~20 항목 모두 10~20배 희석 용액에 살균 효과가 나타냈으며, 세라믹 볼을 용질로 제조된 C는 20종류의 병원균 중 1번과 2번의 병원균만 20배 희석용액에 살균효과가 나타났으나, 3번에서 20번 병원균에는 살균 효과가 없었다.In Table 2 as described above, 0.05 means a 20-fold dilution solution, and 0.10 means a 10-fold dilution solution. As shown in Table 2, A and B prepared by blending plants showed bactericidal effect in dilute solutions of 10-20 times in all pathogens 1-20 items, and C prepared in solutes with ceramic balls was # 1 among 20 pathogens. Only 2 and 2 pathogens showed bactericidal effect in 20-fold dilutions, but 3 and 20 pathogens had no bactericidal effect.

한편, 본 발명의 제조방법에 의해 제조된 살균액을 한국화학연구소 안전성연구센터에 신토불이란 이름으로 토끼에 있어서 피부자극의 독성 시험을 의뢰하였으며, 그 결과는 다음과 같다.On the other hand, the sterilization solution prepared by the manufacturing method of the present invention was commissioned to the toxicity test of skin irritation in rabbits under the name of Shintobul to the Safety Research Center of the Korea Chemical Research Institute.

[실험예 1]Experimental Example 1

시험번호 : N00065Test Number: N00065

시험제목 : 신토불이의 토끼에 있어서 피부자극시험Test Title: Skin irritation test in rabbits

1. 시험군의 구성1. Composition of test group

건강한 수컷의 토끼 3마리를 이용하여 1군으로 구성하였으며, 각 토끼의 경배부에 약 2.5cm * 2.5cm 정도의 크기로 척추를 중심으로 좌우 각각 2개소를 설정하여 좌측구획은 무처치 대조구획으로, 우측에는 시험물질을 도포하는 처치구획으로 정하여 실시하였다.Three rabbits of healthy males were used to make up a group, and each rabbit's cervical part was about 2.5cm * 2.5cm in size. On the right side, the treatment compartment for applying the test substance was determined.

2. 시험물질의 조제 및 투여2. Preparation and Administration of Test Substance

1) 조제법1) Recipe

원제를 그대로 사용하였다.The original agent was used as it is.

2) 투여방법2) Administration method

시험물질의 적용 24시간 전에 제모를 실시하여 다음과 같이 처치구획 및 대조구획으로 구분하였다. 투여방법은 시험물질을 동물 1마리당 찰과피부 2개소와 비찰과피부 2개소로 하여 찰과피부 1개소와 비찰과피브 1개소에 각각 0.5ml씩을 도포하였으며, 무처치 대조구획에는 처치하지 않고 그대로 도포하였다. 도포후 적용부는 가제로 덮은 후 비자극성 테이프로 잘 고정하여 24시간 적용시켰다. 적용기간 종료후에는 미온수를 이용해 도포부를 가볍게 세정해 주었다.Hair removal was performed 24 hours before application of the test substance and divided into treatment compartment and control compartment as follows. For the administration method, 0.5 ml of each of the abrasion skin and one of the abrasion skin was applied to each of the abrasion skin and the abrasion skin with 2 test skins per animal. Applied. After application, the application part was covered with gauze, fixed with a non-irritating tape, and applied for 24 hours. At the end of the application period, the coated part was lightly cleaned with lukewarm water.

3) 관찰 및 검사항목3) Observation and Inspection Items

(1) 일반증상관찰(1) General symptoms observation

시험물질 적용후 72시간까지 외관, 사료섭취, 물섭취 및 일반증상 등에 관하여 매일 관찰하였다.The appearance, feed intake, water intake and general symptoms were observed every day until 72 hours after application of the test substance.

(2) 체중측정(2) weight measurement

시험물질의 적용시 및 적용 후 24시간, 72시간에 측정하였다.Measurements were made at the time of application and at 24 hours and 72 hours after application.

(3) 적용부의 관찰(3) Observation of applied part

시험물질 적용종료후 적용부의 patch를 제거하여 24시간 및 72시간째에 홍반과 가피형성 및 부종형성등의 자극성 유무를 관찰하였다.After application of the test substance, the patch of the applied part was removed and irritation such as erythema, crust formation and edema formation was observed at 24 and 72 hours.

4) 피부반응의 평가 및 자극성의 판정4) Evaluation of Skin Response and Determination of Irritation

피부반응의 평가는 "의약품 등의 독성기준"을 이용하여 평가하였다. 또한, 결과에 대한 자극성의 정도판정은 일반적으로 많이 이용되는 Draize의 P.I.I(Primary Irritation Index)의 산출방법에 따랐다. 이를 표 3 에 나타내었다.Evaluation of skin reaction was carried out using "toxicity criteria such as drug". In addition, the determination of the degree of stimulus for the results was based on the calculation method of the commonly used Primary Irritation Index (P.I.I) of Draize. This is shown in Table 3.

Figure kpo00003
Figure kpo00003

5) 결과5) Results

(1) 일반증상 및 사망율(1) general symptoms and mortality

모든 동물에 있어서 어떠한 일반증상도 관찰되지 않았으며, 사망동물도 발견되지 않았다. 그 결과를 표 4, 5 에 나타내었다.No general symptoms were observed in all animals and no dead animals were found. The results are shown in Tables 4 and 5.

(2) 체중변화(2) weight change

시험물질 적용후 24시간째에 2예의 동물에서 경미한 체중감소가 관찰되었으나 시험물질 적용시 보정과 반창고 등에 따른 스트레스에 의한 일시적인 변화로서 시험물질의 투여와는 관련이 없는 것으로 사료되며, 시험물질 적용후 72시간째 체중을 측정한 결과 모든 동물에서 정상적인 체중증가를 나타내었다. 그 결과를 표 6 에 나타내었다.A slight weight loss was observed in two animals at 24 hours after application of the test substance, but it is considered to be a temporary change due to stress due to correction or band-aid when the test substance is applied and is not related to the administration of the test substance. Body weight measurements at 72 hours showed normal weight gain in all animals. The results are shown in Table 6.

(3) 적용부의 관찰(3) Observation of applied part

모든 동물의 경우 적용부의 관찰은 시험물질 적용 종료후 24시간 및 72시간째에 각각 실시한 결과 홍반, 가피형성 및 부종은 전혀 관찰되지 않았고, 모든 동물의 무처치 대조구획에서는 특이적인 피부반응의 변화로 관찰되지 않았다. 이상의 결과에서 Draize의 P.I.I(Primary Irritation Index)의 산출에 의한 피부1차 자극율이 "0"으로 평가되었다. 그 결과를 표 7 에 나타내었다.In all animals, the observation of the application part was performed 24 hours and 72 hours after application of test substance, respectively. No erythema, no crust formation, and no edema were observed. Not observed. In the above results, the primary skin irritation rate of Draize's P.I.I (Primary Irritation Index) was evaluated as "0". The results are shown in Table 7.

Figure kpo00004
Figure kpo00004

Figure kpo00005
Figure kpo00005

Figure kpo00006
Figure kpo00006

Figure kpo00007
Figure kpo00007

한편, 본 발명의 살균액 제조방법의 순서를 설명하면 다음과 같다.On the other hand, when explaining the procedure of the sterilizing liquid production method of the present invention.

도 2 및 도 3을 참조하면, 우선, 단계(S200)에서는 용기(100)내에 약 1000cc 정도를 담고, 이에 다시 약 1kg 정도를 용해시킨 후 단계(S210)로 진행한다.2 and 3, first, in step S200, about 1000 cc is contained in the container 100, and about 1 kg is dissolved in the container 100, and the process proceeds to step S210.

단계(S210)에서는 용기(100) 내에 용질로써의 식용식물(110), 예를 들면 어성초의 잎줄기, 유자의 열매, 설련화의 꽃, 인삼 등을 각각 또는 두개 이상을 상호 혼합하여 50~100g 정도를 잘게 썰어 투입한 후 단계(S220)로 진행한다.In step S210, the edible plant 110 as a solute in the container 100, for example, leaf stem of citrus vinegar, fruit of citron, flower of vulgaris, ginseng, etc., each or two or more of each other to mix 50 ~ 100g Finely chopped and then proceed to step (S220).

단계(S220)에서는 용기(100)를 통기성 천(120)으로 밀봉하여, 용기(100)를 15~20℃에서 15~20일 동안 보관 발효시켜서 용기(100)내의 수소이온지수를 약 2.7이하로 만든 후 단계(S230)로 진행한다.In step S220, the container 100 is sealed with a breathable cloth 120, and the container 100 is stored and fermented at 15 to 20 ° C. for 15 to 20 days so that the hydrogen ion index in the container 100 is about 2.7 or less. After making, the process proceeds to step S230.

여기에서 수소 이온지수를 약 2.7 이하 정도로 하는 이유는 살균성을 강하게 하기 위해서이다.The reason why the hydrogen ion index is about 2.7 or less here is to enhance sterilization.

단계(S230)에서는 용기(100)내의 찌꺼기를 걸러낸 후, 액만을 취하여 강산성액을 얻게 되는 것이다.In step S230, after filtering the residue in the container 100, only the liquid is taken to obtain a strong acid solution.

본 발명은 도면에 도시된 일실시예를 참고를 설명되었으나 이는 예시적인 것에 불과하며, 당해 기술분야에 통상의 지식을 지닌 자라면 이로부터 다양한 변형 및 균등한 타실시예가 가능하다는 점을 이해할 것이다.Although the present invention has been described with reference to one embodiment shown in the drawings, this is merely exemplary, and it will be understood by those skilled in the art that various modifications and equivalent other embodiments are possible therefrom.

예를 들면, 본 발명에서는 물, 당, 식물 수확물의 양을 일부 한정하여 설명하였으나 본 발명의 취지에 벗어나지 않는 한 그 이상과 이하라도 무방하다.For example, the present invention has been described with a limited amount of water, sugar and plant harvest, but may be more or less as long as it does not depart from the spirit of the present invention.

그리고, 수소 이온지수 값 및 발효 온도, 시간을 한정하였으나 이 또한 본 발명의 취지에 벗어나지 않는 한도에서 변형 가능하다.In addition, the hydrogen ion index value, the fermentation temperature and time are limited, but this can also be modified without departing from the spirit of the present invention.

또한, 본 발명에서는 용기(100)내의 물에 설탕 또는 포도당 1kg 정도를 용해시킨 다음, 식물 수확물(110)을 투입하는 것을 설명하였으나, 용질로써 식용식물(110) 대신에 동물성 체성분, 예를 들면, 우유 등을 투입하여 기능성 음료성으로 사용할 수도 있음은 물론이다.In addition, the present invention has been described to dissolve about 1kg of sugar or glucose in water in the container 100, and then to add the plant harvest 110, but instead of the edible plant 110 as a solute animal body components, for example, Of course, it can also be used as a functional beverage by adding milk.

본 발명에 의해 제조된 살균액의 살균효과 및 피부자극에 대한 한국화학연구소의 시험결과는 첨부자료로 제출한다.The test results of the Korea Research Institute of Chemical Technology on the bactericidal effect and skin irritation of the sterilizing solution prepared by the present invention shall be submitted as an attachment.

이상에서와 같이 본 발명의 살균액은 발효된 제품이므로 시간이 흘러도 부패되지 않으며, 또한 기능이 저하되지 않는 효과가 있다.As described above, since the sterilizing solution of the present invention is a fermented product, it does not decay over time, and there is an effect that the function does not decrease.

그리고, 햇빛에 장시간 노출되어도 살균기능에는 전혀 영향을 받지 않는 특성이 있으며, 살균력이 강해서 소독용으로 월등한 효과가 있다.In addition, the sterilization function is not affected at all even when exposed to sunlight for a long time, the sterilization power is strong, there is an excellent effect for disinfection.

또한, 탈모 방지에도 탁월한 효과가 있다.In addition, there is an excellent effect in preventing hair loss.

Claims (2)

물 1000cc 정도 담긴 용기 내에 당 1kg 정도를 용해시키는 단계; 상기 용기 내에 용질로서의 식용식물을 50~100g을 투입하는 단계; 상기 용기를 통기성 천으로 봉한 후, 상기 용기를 온도 25~30℃에서 15~20일 동안 보관 발효시켜 상기 용기 내의 수소 이온지수(pH)를 2.7이하로 하는 단계; 및 상기 용기에서 찌꺼기를 걸러내어 액만을 얻는 단계를 포함하여 이루어진 살균액 제조방법.Dissolving about 1 kg of sugar in a container containing about 1000 cc of water; Adding 50-100 g of an edible plant as a solute in the container; Sealing the vessel with a breathable cloth, and then storing and fermenting the vessel at a temperature of 25-30 ° C. for 15-20 days to reduce the hydrogen ion index (pH) in the vessel to 2.7 or less; And Sterilizing liquid production method comprising the step of obtaining only the liquid by filtering the residue from the container. 제1항에 있어서, 상기 용질로서의 식용식물은 어성초의 잎, 줄기, 유자의 열매, 설련화 등으로 이루어진 것을 특징으로 하는 살균액 제조방법.The method of claim 1, wherein the edible plant as a solute is made of leaves, stems, citron fruit, vulverization, etc.
KR1019970082320A 1997-12-31 1997-12-31 Process for producing liquid sterilizing agent KR100291572B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019970082320A KR100291572B1 (en) 1997-12-31 1997-12-31 Process for producing liquid sterilizing agent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019970082320A KR100291572B1 (en) 1997-12-31 1997-12-31 Process for producing liquid sterilizing agent

Publications (2)

Publication Number Publication Date
KR19990062015A KR19990062015A (en) 1999-07-26
KR100291572B1 true KR100291572B1 (en) 2001-09-17

Family

ID=37526083

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019970082320A KR100291572B1 (en) 1997-12-31 1997-12-31 Process for producing liquid sterilizing agent

Country Status (1)

Country Link
KR (1) KR100291572B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210129918A (en) * 2020-04-21 2021-10-29 주식회사 한강메디컬 Sterilizing water and device including hypochlorous acid water and manufacturing method of the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210129918A (en) * 2020-04-21 2021-10-29 주식회사 한강메디컬 Sterilizing water and device including hypochlorous acid water and manufacturing method of the same
KR102404959B1 (en) * 2020-04-21 2022-06-07 김인수 Sterilizing water and device including hypochlorous acid water and manufacturing method of the same

Also Published As

Publication number Publication date
KR19990062015A (en) 1999-07-26

Similar Documents

Publication Publication Date Title
KR101812553B1 (en) Composite peptide selenoprotein nutrient solution, preparation method and application thereof
KR101054378B1 (en) Herbal soap composition and its manufacturing method
CN106721829A (en) Pectase method for preparing beverage
KR101715163B1 (en) Food composition for eliminating halitosis comprising kimchi lactic acid bacteria fermented solution of pine cone and pine needles extract
KR102198872B1 (en) Centella asiatica aseptic aging solution with the skin calming and repair effects using a mineral and its Preparation method
KR20150075598A (en) A method for preparation of mixed composition with fermented medical plants extracts
US20240164421A1 (en) Naturally fermented composition of pollen using glacial snow water and preparation method therefor
KR20020084970A (en) Healthy fermentation food using natural medicinal plants and process for preparation thereof
KR20130058877A (en) Perm chemical composition and its manufacturing method
KR20150029672A (en) My skin health and beauty using the functional bio-ceramic and 107 kinds of native grasses
KR100401473B1 (en) Manufacturing method of a ferment drink using Sambakcho and Eosungcho
KR101920859B1 (en) Composition Containing Fermented Vinegar of Thistle Flower for Scalp and Hair, and Method for Manufacturing the Same
KR20030069500A (en) The preparation of fermentation Fig-vinegar using korean drug agent and fig fruit
KR100291572B1 (en) Process for producing liquid sterilizing agent
KR20210013501A (en) Eryobotrya japonica leaf mineral sugar aging solution having a skin protecting effect and a method for producing the same
KR100785974B1 (en) Deodorant cosmetic composition
KR100768471B1 (en) Manufacturing method of beverage extract using pine cone and pine cone
CN107624464A (en) The method for the crops enriched using far infrared cultivation nutrient composition
CN107162782A (en) Clover specific complex sterilizing concentrated nutrient solution and preparation method thereof
JP2001192342A (en) Method for producing mixture of liquid formulation of concentrated stevia with ripe punica granatum
KR0155667B1 (en) Method for preparing low-temperature fermented liquor containing houttuynia cordat thub as a main ingredient
KR101535635B1 (en) Manufacturing method of Japanese apricot pickles
CN105248741A (en) Bamboo leaf loquat flower tea capable of clearing heat, and preparation method thereof
KR100929932B1 (en) Method for manufacturing garlic wine by fermenting gold nano grape and garlic, and garlic wine produced thereby
CN109497244A (en) A kind of environment-friendly production process of passion fruit candied fruit

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20120302

Year of fee payment: 12

LAPS Lapse due to unpaid annual fee