KR100200331B1 - Feed compositions for rainbow trout - Google Patents
Feed compositions for rainbow trout Download PDFInfo
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- KR100200331B1 KR100200331B1 KR1019970008912A KR19970008912A KR100200331B1 KR 100200331 B1 KR100200331 B1 KR 100200331B1 KR 1019970008912 A KR1019970008912 A KR 1019970008912A KR 19970008912 A KR19970008912 A KR 19970008912A KR 100200331 B1 KR100200331 B1 KR 100200331B1
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- rainbow trout
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- 239000000203 mixture Substances 0.000 title claims abstract description 32
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 title 1
- 235000004347 Perilla Nutrition 0.000 claims abstract description 28
- 241000277275 Oncorhynchus mykiss Species 0.000 claims abstract description 23
- 244000124853 Perilla frutescens Species 0.000 claims abstract 2
- 235000020660 omega-3 fatty acid Nutrition 0.000 abstract description 24
- 229940012843 omega-3 fatty acid Drugs 0.000 abstract description 24
- 239000003921 oil Substances 0.000 abstract description 23
- 239000006014 omega-3 oil Substances 0.000 abstract description 19
- 241000251468 Actinopterygii Species 0.000 abstract description 11
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 40
- 241000229722 Perilla <angiosperm> Species 0.000 description 26
- 235000019198 oils Nutrition 0.000 description 22
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 20
- 235000020669 docosahexaenoic acid Nutrition 0.000 description 20
- 229940090949 docosahexaenoic acid Drugs 0.000 description 20
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 20
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 20
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 20
- 235000014113 dietary fatty acids Nutrition 0.000 description 10
- 229930195729 fatty acid Natural products 0.000 description 10
- 239000000194 fatty acid Substances 0.000 description 10
- 150000004665 fatty acids Chemical class 0.000 description 10
- 210000003205 muscle Anatomy 0.000 description 9
- 235000021323 fish oil Nutrition 0.000 description 7
- 238000002156 mixing Methods 0.000 description 5
- 241000124008 Mammalia Species 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000019625 fat content Nutrition 0.000 description 4
- CYQFCXCEBYINGO-IAGOWNOFSA-N delta1-THC Chemical compound C1=C(C)CC[C@H]2C(C)(C)OC3=CC(CCCCC)=CC(O)=C3[C@@H]21 CYQFCXCEBYINGO-IAGOWNOFSA-N 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 235000020665 omega-6 fatty acid Nutrition 0.000 description 3
- 229940033080 omega-6 fatty acid Drugs 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 2
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 231100000517 death Toxicity 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 235000004626 essential fatty acids Nutrition 0.000 description 2
- 238000009313 farming Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 208000019622 heart disease Diseases 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 150000003180 prostaglandins Chemical class 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 241000024188 Andala Species 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 229940013317 fish oils Drugs 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000008629 immune suppression Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- 235000021388 linseed oil Nutrition 0.000 description 1
- 239000000944 linseed oil Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000021315 omega 9 monounsaturated fatty acids Nutrition 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000001335 perilla frutescens leaf extract Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002250 progressing effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 208000023516 stroke disease Diseases 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K97/00—Accessories for angling
- A01K97/04—Containers for bait; Preparation of bait
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K97/00—Accessories for angling
- A01K97/04—Containers for bait; Preparation of bait
- A01K97/045—Preparation of bait; Ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
본 발명은 무지개송어용 사료 조성물에 관한 것으로, 더욱 상세하게는 종래 양식어류의 사료로 사용되지 않던 식물유인 들깨유를 사료 조성물 총량에 대하여 5∼15중량%의 양으로 첨가배합한 사료 조성물에 관한 것으로, 본 발명의 사료 조성물은 무지개송어에 급여시킴으로써 오메가-3 지방산의 함량을 약 2배이상 증가시킬 수 있다.The present invention relates to a feed composition for rainbow trout, and more particularly, to a feed composition in which perilla oil, which has not been used as a feed for aquaculture fish, is added and mixed in an amount of 5 to 15% by weight based on the total amount of the feed composition. The feed composition of the present invention may increase the content of omega-3 fatty acids by about two times or more by feeding rainbow trout.
Description
본 발명은 무지개송어용 사료 조성물에 관한 것으로, 더욱 상세하게는 들깨유를 사료 조성물 총량에 대하여 5∼15중량%의 양으로 함유함을 특징으로 하는 사료 조성물에 관한 것으로, 이를 무지개송어에 급여시킴으로써 오메가-3 지방산, 특히 DHA 또는 EPA의 함량을 증가시킬 수 있는 무지개송어용 사료 조성물에 관한 것이다.The present invention relates to a feed composition for rainbow trout, and more particularly to a feed composition containing perilla oil in an amount of 5 to 15% by weight based on the total amount of the feed composition, by feeding it to rainbow trout A feed composition for rainbow trout that can increase the content of omega-3 fatty acids, in particular DHA or EPA.
우리나라를 포함하여 현재 선진국에서는 각종 질환에 의한 사망원인중 가장 큰 비율을 차지하는 것이 동맥경화증과 같은 심장혈관계 질환으로 기록되고 있는데, 1980년대 이후 식생활 개선과 생활환경 변화 등에 따라서 전체 사인중에서 반이상이 암, 뇌졸증, 심장계 질환 등으로 나타나고 있다. 따라서, 최근 의학 생물학계에서는 프로스타그란딘에 관한 연구를 통하여 혈관반응, 염증, 혈소판 기능, 조혈, 면역 억제기능 등에 대해서 오메가 지방산의 다채로운 인체활성 작용이 알려지고 있으며, 정상 혹은 병적인 많은 장기에 있어서 중요한 역할을 하고 있음이 밝혀졌다. 이 프로스타그란딘의 전구체가 바로 오메가-3 계열과 오메가-6 계열 지방산이라는 점에서 지금까지의 지방섭취의 개념을 새롭게 하는 계기가 되었다. 그 중 오메가-3 계열과 오메가-6 계열 지방산은 오메가-9 계열 지방산과 달리 체내에서 다른 지방산으로부터 생합성되지 않으므로 보통 필수지방산이라고 보고 있는데, 특히 포유동물에서는 오메가-6 계열과 오메가-3 계열의 상호변환이 불가능하므로 각각이 필수구성 요소이다(Bang 등, 1976).In advanced countries including Korea, the most common cause of death from various diseases is cardiovascular diseases such as arteriosclerosis. Since the 1980s, more than half of all deaths have been caused by the improvement of dietary life and changes in living environment. , Stroke and heart disease. Therefore, recently, the medical biology community has known various human activities of omega fatty acids in vascular reaction, inflammation, platelet function, hematopoiesis, immune suppression function through research on prostaglandin, and play an important role in many organs that are normal or pathological. Turned out to be. The prostaglandin precursors are omega-3 and omega-6 fatty acids, which has helped to renew the concept of fat intake. Among them, omega-3 and omega-6 fatty acids are usually considered essential fatty acids because they are not biosynthesized from other fatty acids in the body, unlike omega-9 fatty acids. Especially in mammals, omega-6 and omega-3 fatty acids Since conversion is impossible, each is an essential component (Bang et al., 1976).
이중 오메가-3 계열 지방산이라 함은 필수지방산인 α-리놀렌산(ALA)을 출발물질로하여 포유동물의 체내에서 생합성되어지는 중간대사산물을 포함한 모든 대사지방산을 말하는 것으로, ALA, DHA(Docosahexaenoic acid), EPA(Eicosapentaenoic acid) 등이 있다. 그러나 똑같은 오메가-3 지방산임에도 불구하고 DHA나 EPA가 어유(魚油)에 많이 들어있는 것에 비해서 ALA는 주로 식물성 플랑크톤, 야채 등에 많으며 종실유인 들깨유(63%), 아마인유(50%), 유채(11%) 등에 특히 많이 들어있는 것으로 알려져 있다.Of these, omega-3 fatty acid refers to all metabolic fatty acids including intermediate metabolites that are biosynthesized in the body of mammals using α-linolenic acid (ALA), an essential fatty acid, and ALA, DHA (Docosahexaenoic acid). And EPA (Eicosapentaenoic acid). However, despite the same omega-3 fatty acids, ALA is mainly found in phytoplankton and vegetables, while DHA and EPA are found in fish oils. Perilla seed oil (63%), linseed oil (50%) and rapeseed ( 11%) is known to have a lot.
오메가-3 지방산의 생리기능은 혈액 콜레스테롤 저하효과를 통한 각종심장계 질환의 예방효과, 항종양효과, 기억학습능력의 저하예방효과 등이 알려져 있고, 이 효과들은 동물실험에 의해서 검증되어 있다. 특히, DHA 또는 EPA는 뇌세포를 구성하는 성분으로 보고되어 최근 각종 기호성 식품에서 이용되고 있으며, 이들 기호성 식품에 대한 연구가 활발히 진행되고 있다. 본 발명은 이러한 연구의 결과로서 제공되는 것이며, 본 발명에서는 오메가-3 지방산의 공급형태로서 무지개송어를 선정하여, 무지개송어에 있어서 오메가-3 지방산의 함량을 보다 증가시킬 수 있는 방법을 연구하게 되었다.The physiological function of omega-3 fatty acids is known to prevent various heart disease, antitumor effect and prevention of memory learning ability through blood cholesterol lowering effect. These effects have been verified by animal experiments. In particular, DHA or EPA has been reported as a component constituting the brain cells, and recently used in various palatable foods, research on these palatable foods is actively progressing. The present invention is provided as a result of this research, and in the present invention, rainbow trout was selected as a supply form of omega-3 fatty acids, and a method of increasing the content of omega-3 fatty acids in rainbow trout was studied. .
무지개송어는 담수에서 주로 양식하고 있지만, 해수에서 양식하는 경우에는 담수에 비하여 성장속도가 약 3배나 빠르고 대형으로 자라기 때문에 고가의 어류로 취급되므로 최근에 해수양식이 급진적으로 시도되고 있는 실정이다. 그런데, 어류를 포함하여 해양에서 서식하는 동물은 정상적으로 생육하기 위해서는 DHA나 EPA의 섭취가 필수적이며, 따라서, 해수어류를 양식하는 경우에는 DHA나 EPA가 풍부한 고가의 어유를 첨가한 사료를 급여시키고 있다. 이러한 이유로 사료첨가용 어유의 수요가 최근 크게 증가하고 있다. 그러나, 어유는 고가이므로 경제적 측면에서 대량으로 실시할 수 있는 방법이 아니며, 따라서, 무지개송어에 있어서 오메가-3 지방산, 특히 DHA나 EPA의 함량을 증가시킬 수 있는 새로운 사료 조성물에 대한 개발이 절실히 요구되고 있다.Rainbow trout is mainly farmed in freshwater, but when farmed in seawater, it grows about three times faster and grows larger than freshwater, so it is treated as expensive fish. However, inhabitants of the sea, including fish, are required to consume DHA or EPA in order to grow normally. Therefore, in the case of farming seawater fish, a diet containing expensive fish oil rich in DHA or EPA is being fed. . For this reason, the demand for fish oil for feed has recently increased significantly. However, since fish oil is expensive, it is not an economically viable method. Therefore, there is an urgent need for the development of a new feed composition that can increase the content of omega-3 fatty acids, especially DHA or EPA, in rainbow trout. It is becoming.
이에, 본 발명자들은 오메가-3 지방산의 공급을 목적으로 해수어류 양식용 사료에 배합되어 사용해온 어유를 대체할 수 있는 새로운 오메가-3 지방산 공급체를 찾기 위해서 연구를 거듭하던 중, 포유동물의 체내에서 이루어지는 오메가-3 지방산의 대사과정을 해수어류에 적용하는 경우에도 ALA를 출발물질로 하여 EPA나 DHA의 생합성이 가능한지에 대하여 연구하게 되었으며, ALA를 다량으로 함유한 식물유를 사료에 배합하여 급여시킴으로써 양식어류에 있어서 EPA 및 DHA의 함량이 증가됨을 확인하고 본 발명을 완성하게 되었다. 이로써, 종래 해수어류의 양식용으로 사용하지 않던 식물유, 특히 ALA를 다량으로 함유한 들깨유를 어유의 대체물질로서 제공하게 되었다.Therefore, the inventors of the present invention, while searching for a new omega-3 fatty acid supplier that can replace fish oil that has been used in marine fish and fish feed for the purpose of supplying omega-3 fatty acids, the body of the mammal In the case of applying the metabolism of omega-3 fatty acids to sea fish, we studied whether bio synthesis of EPA or DHA is possible by using ALA as a starting material. Confirmed that the content of EPA and DHA in the cultured fish is increased and completed the present invention. As a result, vegetable oil that has not been used for aquaculture of seawater fishes, in particular, perilla oil containing a large amount of ALA is provided as a substitute for fish oil.
따라서, 본 발명의 목적은 무지개송어에 있어서 오메가-3 지방산, 특히 EPA나 DHA의 함량을 증가시킬 수 있는 사료 조성물을 제공하는 것이다.Accordingly, it is an object of the present invention to provide a feed composition capable of increasing the content of omega-3 fatty acids, in particular EPA or DHA, in rainbow trout.
상기한 목적을 이루기 위해서, 본 발명에 따른 무지개송어 양식용 사료 조성물은 사료 조성물 총 중량에 대하여 들깨유 5∼15중량%를 함유하는 것을 특징으로 한다.In order to achieve the above object, the rainbow trout culture feed composition according to the present invention is characterized in that it contains 5 to 15% by weight perilla oil relative to the total weight of the feed composition.
본 발명의 다른 목적 및 작용은 하기 발명의 상세한 설명으로부터 당업자에게 명백하게 드러날 것이다.Other objects and functions of the present invention will become apparent to those skilled in the art from the following detailed description of the invention.
이하 본 발명을 보다 구체적으로 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명에 따른 사료 조성물은 무지개송어 양식용으로 종래 사용되어온 사료 조성물에 들깨유를 추가로 더 배합하여 이루어진 것이다.Feed composition according to the present invention is made by further blending the perilla oil in the feed composition has been conventionally used for rainbow trout farming.
본 발명에서 들깨유를 배합하여 사료 조성물을 조성하는 종래 무지개송어용 사료 조성물은 특별히 한정되지 않으며, 일례로 하기 표 1의 조성을 갖는 사료 조성물에 배합하여 사용할 수 있다.Conventional rainbow trout feed composition for blending perilla oil in the present invention to form a feed composition is not particularly limited, it can be used in combination in the feed composition having a composition of Table 1 as an example.
[표1]Table 1
일반사료의 조성General feed
또 본 발명에서 무지개송어에 있어서 오메가-3 지방산 특히 EPA나 DHA의 함량을 증가시키기 위하여 공급하는 들깨유는 하기 표 2의 지방산 조성에서 보는 바와 같이 ALA를 63%나 함유하고 있다.In the present invention, perilla oil supplied to increase the content of omega-3 fatty acids, particularly EPA or DHA in the rainbow trout contains 63% of ALA as shown in the fatty acid composition of Table 2 below.
[표2][Table 2]
들깨유의 지방산 조성Fatty Acid Composition of Perilla Oil
즉, 들깨유에 다량 함유된 ALA는 무지개송어에 사료로서 급여되어 포유동물의 체내 대사과정과 유사하게 EPA를 거쳐 DHA로 대사됨으로써 근육중에 오메가-3 지방산, 특히 EPA나 DHA의 함량이 증가하는 것으로 그 기작을 예측할 수 있다.That is, ALA contained in large amounts of perilla oil is fed to rainbow trout and is metabolized to DHA via EPA, similar to the metabolic processes in mammals, thereby increasing the content of omega-3 fatty acids, especially EPA or DHA, in the muscle. Predict mechanism.
이러한 들깨유는 목적하는 효과와 경제적측면을 고려하여, 본 발명에서는 사료 조성물 총 중량에 대해 5∼15중량%의 양으로 배합된다. 들깨유는 들깨유의 형태로 배합될 수 있지만, 들깨 또는 폐기되고 있는 들깨박의 형태로 배합될 수 있으며, 이들의 혼합물로서 배합될 수도 있다. 어떤 형태이든지 들깨유의 함량이 5∼15중량%가 되도록 첨가배합한다.Such perilla oil is blended in an amount of 5 to 15% by weight based on the total weight of the feed composition in consideration of the desired effect and economic aspects. Perilla oil may be blended in the form of perilla oil, but may be blended in the form of perilla or perilla which is being discarded, or may be blended as a mixture thereof. In any form, add and mix so that the content of perilla oil is 5-15% by weight.
본 발명에 의하면, 고가의 어유첨가 없이도 저비용으로 무지개송어에 있어서 오메가-3 지방산의 함량을 크게 증가시킬 수 있으므로 건강식품을 선호하는 현대인의 경향에 부응할 수 있다.According to the present invention, since the content of omega-3 fatty acids in rainbow trout can be greatly increased without expensive fish oil addition, it can meet the trend of modern people who prefer health food.
이하 실시예를 들어 본 발명을 보다 상세하게 설명하지만 본 발명이 이들 실시예에만 한정되는 것은 아니다.The present invention will be described in more detail with reference to the following Examples, but the present invention is not limited to these Examples.
[실시예 1]Example 1
급여조건(들깨유의 혼합조건 및 급여기간)에 따라서 무지개송어 근육의 지방함량을 측정하고, 그 결과를 표 3에 나타내었다.The fat content of rainbow trout muscle was measured according to feeding conditions (mixing condition and feeding period of perilla oil), and the results are shown in Table 3.
[표3]Table 3
들깨유 혼합조건, 급여기간에 따른 무지개송어 근육의 지방함량Fat Contents of Rainbow Trout Muscle According to Perilla Oil Mixing Conditions and Feeding Period
표 3에서 알 수 있는 바와 같이, 지방 함량은 들깨유 혼합비율을 15%로 하여 1개월 급여시 3.6%로 시중사료(전북 군산에 위치한 푸리나코리아사의 송어육성용 펠렛) 급여시 2.7%에 비해 0.9% 증가하였고, 5% 급여시 3.2%로서 0.5% 증가하여 들깨유 첨가량의 증가에 따른 지방함량의 증가는 크지 않았다.As can be seen from Table 3, the fat content was 15% perilla with a perilla oil mixing ratio of 3.6% per month, compared to 2.7% for commercial feed (purina pellets made by Purina Korea, located in Gunsan, Jeonbuk). The increase in fat content was increased by 0.9% and 0.5% by 3.2% at 5% supplementation.
[실시예 2]Example 2
참고예 2의 사료에 들깨유를 표 4에 기재된 바대로 첨가하여 1개월간 급여시 무지개송어 근육의 지방산 조성을 조사하였다. 그 결과를 표 4에 나타내었다. 근육에 함유된 총지방산중 오메가-3 지방산 함량은 가스크로마토그래피(GC-14A, schimadzu사)에서 길이 25m, 내경 0.5mm의 캐필러리컬럼을 이용하여 전체 지방산 함량을 측정하고, 그중 총 오메가-3 지방산의 함량을 계산함으로써 산정된 것이다.Perilla oil was added to the feed of Reference Example 2 as described in Table 4 to investigate the fatty acid composition of the rainbow trout muscle when fed for 1 month. The results are shown in Table 4. The total fatty acid content of omega-3 fatty acids in muscle was measured by gas chromatography (GC-14A, schimadzu) using a capillary column of 25m in length and 0.5mm inner diameter. 3 Calculated by calculating the content of fatty acids.
[표4]Table 4
들깨유첨가 급여농도에 따른 무지개송어 근육의 지방산 조성(1개월 사육)Fatty Acid Composition of Rainbow Trout Muscle According to Perilla Seed Concentration (1 Month Breeding)
표 4에서 알 수 있는 바와 같이, 참고예 2의 사료인 시판사료(전북 군산에 위치한 푸리나코리아사의 송어육성용 펠렛)에 들깨유를 5, 10, 15% 첨가하여 무지개송어에 급여한 결과 급여농도가 높을수록 오메가-3 지방산 총함량이 증가되었다. 따라서, 15% 급여시 α-리놀렌산에서 EPA 및 DHA로의 전이율도 높은 것으로 나타났다. 또한, 15% 혼합시 오메가-3 지방산 총함량이 근육 100g중에 1,030.3㎎으로 시판사료 404.7㎎에 비해서 2.5배정도 증가됨을 알 수 있다. 한편 EPA 함량은 32.9에서 50.6㎎, DHA 함량은 339.3㎎에서 396.4㎎으로 증가됨을 알 수 있다.As can be seen in Table 4, 5, 10, 15% of perilla oil was added to the commercial feed (Ref. Higher concentrations increased the total content of omega-3 fatty acids. Hence, the 15% feeding also showed a high rate of transition from α-linolenic acid to EPA and DHA. In addition, it can be seen that the total content of omega-3 fatty acids in 15% of the mixture increased by 1,030.3 mg in 100 g of muscle to 2.5 times that of the commercial feed 404.7 mg. Meanwhile, it can be seen that the EPA content was increased from 32.9 to 50.6 mg and the DHA content was increased from 339.3 mg to 396.4 mg.
[실시예 3]Example 3
들깨유를 15중량%로 첨가하여 1개월 및 2개월 급여한 경우의 근육에 있어서 오메가-3 지방산 함량을 조사하여 그 결과를 표 5에 나타내었다.The content of omega-3 fatty acids in the muscles of 1 and 2 months of supplemented perilla oil at 15% by weight was investigated and the results are shown in Table 5.
[표5]Table 5
급여 기간별 무지개송어 근육의 지방산 조성Fatty Acid Composition of Rainbow Trout Muscle by Feeding Period
표 5에서 알 수 있는 바와 같이, 들깨유를 15중량%의 양으로 첨가한 사료를 1개월 급여한 실험구는 1,030㎎, 2개월 급여한 실험구는 1,342.9㎎의 오메가-3 지방산의 함량을 나타내지만, EPA 및 DHA의 함량증가는 매우 낮아서 들깨유를 함유한 시료를 무지개송어에 1개월 전후로 하여 급여하는 것이 경제적인 측면에서 바람직한 것으로 나타났다.As can be seen from Table 5, the experimental group fed 1 month feed of perilla oil in 15% by weight of 1,030 mg, the experimental group fed 2 months showed 1,342.9 mg of omega-3 fatty acid content, The increase in the content of EPA and DHA was so low that it was economically desirable to feed perilla oil containing samples perilla for about 1 month to rainbow trout.
상기한 설명에서 알 수 있는 바와 같이, 종래에는 어류를 포함하여 해양에서 서식하는 동물들의 정상적인 생육을 위해서 EPA 및 DHA가 함유된 고가의 어유를 포함한 사료를 급여하였지만, 본 발명에서는 EPA 및 DHA를 함유하지 않으므로 어류의 사료로 사용되지 않던 식물유, 즉 들깨유를 사료 조성물 총량에 대하여 5∼15중량%의 양으로 첨가하여 무지개송어를 생산하므로 경제적인 측면에서 효과적이다. 또한, 들깨유를 함유한 사료를 무지개송어에 급여하면 EPA 및 DHA 등의 오메가-3 지방산의 함량이 시판되는 사료를 급여한 경우보다 2배이상 증가하므로 건강식품을 선호하는 현대인의 요구에 부응할 수 있다.As can be seen from the above description, conventionally, a feed containing expensive fish oil containing EPA and DHA was fed for the normal growth of animals inhabiting the ocean including fish, but the present invention contained EPA and DHA. Since it is not used as a feed of fish, vegetable oil, that is, perilla oil is added in an amount of 5 to 15% by weight based on the total amount of the feed composition to produce rainbow trout, so it is effective economically. In addition, feeding feed containing perilla oil to rainbow trout increases the content of omega-3 fatty acids, such as EPA and DHA, more than twice that of commercially available feeds, thus meeting the needs of modern people who prefer health foods. Can be.
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