KR0173814B1 - Health aid food comprising the extract of rosa rugosa thunb - Google Patents

Abstract

The present invention relates to a blood glucose lowering agent extracted from the root of the glycolysis and a method for producing the same. The present invention is a mixture of hot water extract and methanol or ethanol                 It is a hypoglycemic agent that contains the extraction residue after extraction with butanol. The extraction residue after extraction with butanol not only has an excellent effect on lowering blood sugar,                 Eggplant is a complete removal of toxic substances.

Description

            Dietary Supplements Containing Glycerium Root Extract         

The present invention relates to a hypoglycemic agent extracted from the herbal medicine, more specifically, a hypoglycemic agent extracted from the root of the glycolysis and a method for producing the same.

Rosa rugosa Thunb is a deciduous shrub that grows on sandy beaches and foothills, vertically below 1600m, horizontally in Jeonnam,                 It is distributed in Gyeongbuk, Chungnam, Gangwon, Gyeonggi, Yellow Sea, Pyeongan, North Hamnam, and Japan, Manchuria, and Kamchatka (Jung Tae-hyun, Korean Plant Book, Wood Headquarters, Lee Mun-sa, p.177,                 1974). The pharmacological use of this plant is used for flowers, bleeding, wind, and excessive menstruation.                 It has been used for the treatment (Yang, Han Seok et al., Journal of Pharmacy, 31 (6), 394 (1987)).

Some studies on the hypoglycemic effect of the plant have been reported. Song (Song Sun Doo and two others, Yonsei University Medical School, 10, 125 (1977))                 The hypoglycemic effect was reported by oral administration of hot water extracts from the glycolysis subterranean rats induced hyperglycemia with alloxan.                 Lee, Myung-Yeol (Myung-Yeol Lee, Master's Thesis, Chung-Ang University, 1982, Chosun University, Pharmaceutical Research, 6, 19 (1984))                 It was found that methanol extract and saponin showed hypoglycemic effect when applied to rabbits of experimental hyperglycemia. On the other hand, Yang (Pharmaceutical Journal, 31 (6),                 394 (1987) and others reported that methanol extracts from the root of glycolysis have no effect on blood glucose-enhancing effects but are effective on hyperlipidemia.

As mentioned above, some extracts of the corresponding root or muscle are known to be effective in lowering blood sugar, but they are observed in a short period of about 1 week.                 In addition, most of these effects are thought to stimulate the secretion of β-cells in the pancreatic Langerhans inland and to promote insulin secretion.                 have. Therefore, it is not known whether the hypoglycemic effect of insulin secretion is almost destroyed, that is, insulin dependent diabetes mellitus.                 The effect of long-term treatment remains questionable. In addition, the number of hot water in the periphery and roots that are effective in diabetes in folk medicine                 The extract has been problematic in causing side effects such as vomiting and abdominal pain when taken more than several times.                 The effect was very low or very little.

The present invention, when extracted with butanol in the process of extracting the hot water extract in the roots of the glycolysis roots with various solvents, the toxic substances are removed by the extract and the blood sugar drop in the extraction residue                 The discovery that the dominant component exists is complete based on this finding. Generally contrasted with the general method of extracting the bioactive substance as a solvent.                 will be.

An object of the present invention is to provide a hypoglycemic agent and a method for producing the same, which have no side effects and can be treated with insulin-dependent hyperglycemia using herbal ingredients.

Another object of the present invention to provide a dietary supplement that is effective in lowering blood sugar.

The object of the present invention is to add a lower alcohol such as methanol or ethanol to the hydrothermal extract of the glycolysis root to prepare a soluble component and extract it with butanol.                 It is achieved by a dietary supplement or a hypoglycemic agent having a glycemic-enhancing effect, including the latter residue.

The hypoglycemic agent

1) extracting hot water from the corresponding root;

2) extracting the hot water extract with lower alcohols such as methanol and ethanol to obtain a soluble component;

3) adding and extracting butanol as an extraction solvent to the lower alcohol soluble powder; And

4) prepared by removing the butanol aliquot.

The extraction residue after the butanol extraction is a fraction having an activity of lowering blood sugar.

The glycolytic roots are preferably ground or micronized to extract hot water.

The hot water extract may be used as it is in the next step, but may be lyophilized and powdered before being used in the next step. Which is a low level alcoholic to powder of this hydrothermal extract                 Methanol or ethanol is added to obtain the soluble component. In particular, methanol is preferable. Hexane, chloroform and / or before extraction of this alcohol mixture with butanol                 Ethyl acetate can be extracted depending on the polarity.

Hereinafter, the present invention will be described in detail with reference to Examples. However, the scope of the present invention is not limited by these examples.

Example 1

[Preparation of Glycylated Root Extract]

The root of the flower was purchased from the herbal medicine material in Jeonju. These dried roots are chipped with a band saw and then powdered into a small Willy mill.                 40-80 mesh portions were taken and used as extraction samples. Hot water extraction is equivalent to a 5 liter Erlenmeyer flask. 500 g of root powder (dry weight) and 3 liters of distillation.                 Addition of ion-exchanged water was carried out for 60 minutes while slowly boiling. After extraction, the contents were sufficiently cooled, filtered, and centrifuged to separate the extraction residue and the extract. Residue                 The extract after removal was lyophilized to form a powder. After lyophilization, the weight of the hot water extract was 49.9 g, which was 10% by weight based on the dry weight of the corresponding root powder.                 It corresponded. 5 g (dry weight) of the lyophilized powder of the hydrothermal extract was taken, treated with methanol, stirred and centrifuged. The obtained methanol soluble part is 50 degreeC                 It concentrated under reduced pressure and vacuum-dried below. The amount of methanol soluble part after drying was 3.17 g. Methanol solubles are charged with a small amount of distilled ion-exchanged water for the next extraction.                 Melted. Then they were extracted with hexane, chloroform, ethyl acetate and butanol. Hexane, chloroform and ethyl acetate extracts in each extract are extremely                 It was a small amount, most of which was 1.86 g and 1.13 g after the butanol and butanol extraction, respectively.

Example 2

[Proof of Hypoglycemic Activity Classification]

Examination of the hypoglycemic activity of the hydrothermal extracts of the oxidized root and each of the extracts and residues described above shows the distinctive effect of insulin-dependent diabetic rats.                 Proved.

1) Induction of hyperglycemia and hypoglycemic test

High blood sugar was induced artificially by injecting streptozotocin into experimental mice (150-200 g). These mice were reared for two weeks to fully insulin-dependent.                 150 ml of water containing 2-4 mg of extract and residue were given daily. Blood glucose was measured at most weekly intervals except for the early days. And blood sugar drop                 In the case of the residue after extraction of butanol, which is highly effective, the administration of these residues is stopped and the pharmacological effect                 Persistence was reviewed.

2) test result

The hot water extracts of the well-known roots of glycolysis, which have been widely known in the folk field, showed little hypoglycemic effect in insulin-dependent diabetic rats as shown in Table 1.                 In addition, methanol extract seems to have a slight hypoglycemic effect, but the effect was not recognized in the margin of error. From these results, methanol                 In the case of the extract, the effect was not recognized in insulin-dependent diabetes like the hydrothermal extract. In addition to these extracts, ethyl acetate and butanol extracts                 The hypoglycemic effect was examined, but there was no effect at all.

On the other hand, as shown in Table 1, the residue classification after butanol extraction had a blood sugar reduction of 55% after 1 week of administration, and after 2 weeks, it was almost the same level as that of normal mice.                 Maintained. As a result, the pharmacological action was continued for about 2 weeks, and significant blood glucose levels were recognized at 3 weeks after discontinuation, but again, butanol                 Significant hypoglycemia occurred again one week after the residue was administered.

Example 3

[Acute Toxicity Test]

The acute toxicity test for the residue after butanol extraction prepared in Example 1 was carried out on mice weighing 15-25 g. Divide the mouse into two groups of six                 At 1,500 mg / kg for intraperitoneal injection and 10,000 mg / kg for oral injection, all survived 2 weeks later. Of Example 1                 After butanol extraction, the residue was considered to have little acute toxicity.

Meanwhile, in the case of the butanol extract in Example 1, a substance having low solubility in butanol was precipitated during the vacuum concentration. This precipitate is present in very small amounts relative to the total butanol extract.                 However, when they were administered to the hyperglycemic rats in the same amount as the hypoglycemic experiment, there was a significant hypoglycemic effect.                 A mortality of 50% was shown to qualitatively confirm the presence of strong toxicity in this precipitate.

As shown in the above example, it was found that there was an excellent blood sugar lowering effect in the residue fraction after butanol extraction. Of pharmacological ingredients                 It can be seen that most of them are gathered in this division. The components exhibiting the pharmacological action are not a single component system, but are assumed to act in combination. Butanol                 After the extraction, the hyperglycemic mice maintain normal blood glucose by administering the residue to the hyperglycemic-induced rats for 2 weeks.                 It lasted about two weeks. The fact that such blood glucose lowering and sustaining effects exist in the residue after butanol extraction in the vicinity of glycolysis has not been reported so far. In other words                 This study revealed a characteristic activity that lowers blood glucose in the glycolytic root, which has only been observed to be insignificant.

In addition, as shown in Example 3, the residue which is not extracted with butanol is considered to contain a toxic substance, and this is the conventional folk remedies.                 Decoction will be an indirect explanation for side effects such as stomach pain.

Claims (7)

Methanol or ethanol was added to the hydrothermal extract of the glycolysis root to obtain the alcohol soluble portion, and the extract residue was extracted with butanol.                 Health supplements. The method of claim 1, wherein the hot water extract obtained by extracting with methanol or ethanol is extracted with hexane, chloroform and ethyl acetate before extracting the soluble part with butanol.                 Health supplement comprising an extract residue extracted with one or more solvents selected from the group consisting of. The dietary supplement according to claim 1 or 2, wherein the extract residue is powdery, syrupy or liquid. 1) extracting hot water from the corresponding root; 2) adding methanol or ethanol to the hot water extract to obtain these soluble parts; 3) With extraction solvent in the soluble part                 Adding butanol and 4) A method for producing a health supplement with a butanol extract residue comprising the step of removing the butanol fraction. The method of claim 4, further comprising the step of powdering to extract the hydrolyzed root of the glycolysis. The method of claim 4 or 5, further comprising the step of lyophilizing and powdering the hot water extract after step 1). The method of claim 4 or 5, wherein at least one selected from the group consisting of hexane, chloroform and ethyl acetate between steps 2) and 3).                 Method for producing a health supplement food further comprising the step of extracting as a solvent.