KR0143369B1 - Food composition - Google Patents

Abstract

The present invention relates to a food composition for the prevention, removal and liver protection of a hangover containing bookfish extract and maltol, which is very rapid alcohol through synergistic effect when the two components are used in combination than the case where bookfish extract and maltol are administered alone. It is composed not only to promote acetaldehyde metabolism but also to prevent alcohol detoxification due to hangover, and it is formulated so that one dose of Northfish X and Maltol is 3.0-22.5g of Northfish X and 30-187.5mg of Maltol. Food composition for the prevention, removal and hangover protection of hangover by liquid or powdered alcohols containing one or two or more of vitamins such as niacin, vitamin B ₁ and vitamin C and amino acids such as asparagine salt and glutamate to be.

Description

Food composition

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a food composition, and more particularly to a food composition for preventing, removing, and protecting liver from hangover by alcohol containing X and maltol of dried pollock (hereinafter referred to as "North Korean").

Ethanol, the main ingredient of alcohol, gets into the body and is partially metabolized in the stomach, and most of it is absorbed by the small intestine and moves to the liver. About 20% of alcohol is consumed by alcohol dehydrogenase (ADH) in the stomach, which is oxidized to acetaldehyde to remove some of the toxicity. J. Medicine. 319: 1639,1988). On the other hand, alcohol entering the liver causes various physiological changes, depending on the amount of ethanol, that is, when the blood alcohol concentration is low, such as 0.2-2.0 mM, it is mainly caused by ADH in the cytosol (cell fluid), and the blood level is about 10-50 mM. When the alcohol concentration is high, it is usually oxidized by the action of the microsomal ethanol oxidizing system (MEOS) present in the endoplasmic reticulum to produce acetaldehyde. Alcohol metabolism is also possible with catalase, but in this case, its function is minimal in a steady state, not under conditions in which excess hydrogen peroxide is produced. Acetaldehyde thus produced is known to be oxidized to acetic acid by Aldehyde dehydrogenase (ALDH) (Arch. Biochem. Biohys. 236: 36. 1985).

However, when a large amount of acetaldehyde is produced, headache symptoms such as hot flashes, vomiting, eyeball congestion, and dizziness are primarily experienced and secondary to liver damage. The first symptom is liver fat accumulation, ethanol and acet. In addition to the consumption of nicotinamide adenine dinucleotide (NAD), an alcohol metabolism coenzyme in the oxidation process of aldehyde, a large amount of nicotineamide adenine dinucleotide hydrogen (NADH) is produced and NADH for NAD As the ratio of quantitatively increases, the synthesis of fatty acids in hepatocytes increases.

The second is hepatomegaly, in which acetaldehyde forms covalent bonds with hepatic macromolecules or proteins circulating in blood vessels (albumin, hemoglobin). They are suppressed by their release, resulting in an increase in intracellular proteins, triggering the influx of small ions and water (New Eng. J. Medicine. 319: 1639,1988).

Third, hepatic fat peroxidation. Acetaldehyde accumulated under ethanol oxidation and NAD-deficient conditions may produce superoxide, a free radical, under the action of enzymes in peroxysomes (Biochem. Biophys. 143: 339, 1987), acetaldehyde binds to cysteine or glutathione via a sulfhydryl group (-SH) to reduce glutathione, which is responsible for eliminating the toxicity of free radicals produced in cells Lipids are peroxidized.

Much research has been conducted to eliminate the side effects of ethanol as described above. First, it is experimental that aspartic acid promotes ethanol metabolism by strengthening the malate-aspartate shuttle, which is a coenzyme of intracellular alcohol metabolism. Results have been reported (Korean J. Biochem. 25,137-143,1993), and as a composition patent related to hangover removal, Japanese Patent Laid-Open No. 1-207232 (1989) discloses L-alanine, or L-alanine and α-L-as Partylphenylalanine was the main ingredient, and Japanese Patent Hei 2-124084 (1990) used alanine, glutamine, ornithine and pepper extract, and Japanese Patent Hei 4-342528 (1992) used vitamin B ₂ , B ₆ , C and Cysteine, US Pat. No. 4,444,548 (1985) for vitamins B ₁ and C, cysteine, hesperidin, rutin, and European Patent No. 234464 (1987) for vitamin B ₁ and C and R-NH- Hangover with CH (CH ₂ ) n-R '(COOH) The removal composition is presented.

However, the above-mentioned conventional hangover removal techniques focus on lowering the concentration of acetaldehyde produced by the blood oxidation and primary oxidation of alcohol, which are usually caused by alcohol intake. However, even if it promotes alcohol metabolism, there is a need to completely prevent side effects caused by acetaldehyde accumulated to some extent, and thus there is a need to prevent liver damage caused by this.

The bookfish has been widely used as a material for haejangguk after being drunk from whales in Korea.

The degree of alcohol metabolic effect of the north fish is proved by the experimental result that the amino acid (aspartic acid, alanine, glutamic acid), which is known to have alcohol metabolism effect, is higher than other amino acids.

According to the composition of free amino acids in the North Korean language, 60% of the four amino acids, such as glycine, alanine, glutamic acid, and aspartic acid (Mun Mira, Master's Thesis, Sungshin Women's University, 1986), were analyzed for all amino acids of Northern protein. As a result, it was reported that glutamic acid was the highest, followed by aspartic acid, lysine, leucine, arginine, and alanine (Byung Hwa Yu, Master's Thesis, Sookmyung Women's University, 1963).

In addition, maltol, a red ginseng extract, forms chelates with iron ions, thereby initiating a lipid peroxidation reaction in the nicotineamide adenine dinucleotide phosphate hydrogen (NADPH) -dependent peroxide generation system in microsomes. It has been reported that there is an antioxidant effect of inhibiting the lipid peroxidation reaction of hepatocytes by deodorizing the iron ions needed. (Korean Biochem.

Therefore, while investigating a food composition that can prevent not only hangovers but also secondary symptoms such as peroxidation of hepatocellular fat caused by hangovers, the researchers added maltol to northwest extracts to promote alcohol metabolism, In order to eliminate the secondary symptoms, the effects of the two components were examined in several experimental examples below. When the two components were administered in combination, the concentration of alcohol and acetaldehyde in the blood was remarkable. Of course, the present invention was completed by confirming that the lipid peroxide content in the liver tissue was significantly lowered.

Food composition for the prevention, elimination and liver protection of the hangover of the present invention contains norther extract and maltol as an active ingredient, and if necessary, by adding one or two or more vitamins and amino acids as nutritional supplements, As a vitamin agent, you can choose between niacin, vitamin B ₁ or vitamin C, and as an amino acid agent, you can use asparagine or glutamate.

The bookfish extract used in the present invention was put into purified water to which crushed bookfish was added to reduced sugar sugar, reflux extraction at 90-100 ° C. for 5-10 hours, or the extract obtained by filtering and concentrating the extract extracted 1-2 hours at a frequency of 25-30KHz. As a detailed manufacturing method, reference is made to the specification of Patent Application No. 94-27565, and maltol is currently commercialized and supplied and is easy to purchase.

In carrying out the present invention, the following auxiliary experiments were first performed in order to obtain a preferred content of north fish extract and maltol.

[Subsidiary Experimental Example 1] Manufacturing method of bookfish extract

15% finely chopped, 80% purified water, 5% per reduced rice was mixed and extracted at 90-100 ℃ for 5 hours. The extract was concentrated to 62BRIX and then sterilized and filtered at 100 ° C. for 10 minutes to obtain a transparent bookfish extract.

Secondary Experimental Example 2 Measurement of Changes in Blood Alcohol and Acetaldehyde Levels

After the rats were starved for 18 hours, each test drug was mixed with physiological saline when Buk-X, Maltol and B-tox-X and Maltol of the following comparative example were added and nutritional supplements were added, followed by blood alcohol concentration and acetaldehyde for 2 and 4 hours. The change in concentration was observed.

The following results are average values obtained from the serum of 6 rats, and alcohol and acetaldehyde quantification were measured by gas chromatography (Clinic. Chim. Acta. 87: 175, 1978).

1) Effect of concentration change of North Korean extract (see Table-1)

Significance with Comparative Example 1: *: P 0.05 **: P 0.01

Comparative Example 1: 10ml / Kg of saline solution was prepared.

Comparative Example 2: A physiological saline was added to 500 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 3: A physiological saline was added to 1000 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 4: Physiological saline was added to 1500 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 5: Physiological saline was added to 2000 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 6: Physiological saline was added to 2500 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 7: Physiological saline was added to 3000 mg / Kg of north fish extract to prepare 10 ml / Kg.

As can be seen in the effect test according to the change of the concentration of norther extract alone, Table 1 showed a clear decrease in blood alcohol and acetaldehyde concentration in the group administered norther extract rather than the control of Comparative Example 1, in particular 1000mg / Kg Example 3) From the above, it can be seen that the maximum alcohol metabolic effect was shown.

2) Effect of maltol concentration change (see Table-2)

Significance with Comparative Example 15: * .P0.05 ** P0.01

Comparative Example 8: 10 ml / Kg of saline solution was prepared.

Note 9: Physiological saline was added to 1 mg / Kg of maltol to prepare 10 ml / Kg.

Remark 10: Physiological saline was added to 5 mg / Kg of maltol to prepare 10 ml / Kg.

Note 11: Physiological saline was added to 10 mg / Kg of maltol to prepare 10 ml / Kg.

Remarks Example 12: Physiological saline was added to 15 mg / Kg of maltol to prepare 10 ml / Kg.

Note 13: Physiological saline was added to 20 mg / Kg of maltol to prepare 10 ml / Kg.

Remark 14: Physiological saline was added to 25 mg / Kg of maltol to prepare 10 ml / Kg.

As can be seen in the effect test according to the change of concentration when maltol alone is administered in Table-2, it showed a generally low effect compared to North Korean extract, but showed a certain level of alcohol metabolism than the control of Comparative Example 8, In particular, more than 5mg / Kg it can be seen that the effect on blood alcohol and acetaldehyde metabolism showed the maximum.

3) Combination Effect of North Korean X and Maltol

The combined effect was measured by fixing the north fish extract at 1000mg / Kg and changing the concentration of maltol to 1,5,10,15,20,25mg / Kg.

Comparative Example 15: 10 ml / Kg of saline solution was prepared.

Comparative Example 16: Maltol was added to 10 mg / Kg by adding saline to 5 mg / Kg.

Comparative Example 17: A saline solution was added to 1000 mg / Kg of north fish extract to prepare 10 ml / Kg.

Comparative Example 18 Physiological saline was added to 1000 mg / Kg of Northfish extract and 1 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 19 Physiological saline was added to 1000 mg / Kg of Northfish extract and 5 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 20: A saline solution was added to 1000 mg / Kg of Northfish extract and 10 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 21: A saline solution was added to 1000 mg / Kg of Northfish extract and 15 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 22: Saline was added to 1000 mg / Kg of Northfish extract and 20 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 23: A saline solution was added to 1000 mg / Kg of north fish extract and 25 mg / Kg of maltol to prepare 10 ml / Kg.

As can be seen from the combined effect experiment of Table-3, synergistically rapid alcohol metabolism was shown when the two components were administered in combination than when the northwest extract or maltol was administered alone, in particular maltol 5mg / Kg at 1000mg / Kg When combined with (200: 1 ratio) showed a clear synergistic effect, it can be seen that a specific compounding ratio can be selected within the effective range of maltol 25mg / Kg (40: 1) to 1000mg / Kg of north fish extract as necessary .

4) Effect of adding nutritional supplement

Commonly used nutritional supplements include vitamin preparations and amino acid preparations. Therefore, in the present invention, in order to add nutritional supplements to the composition of the bookfish extract and maltol having an alcohol metabolic effect, several components of the vitamin preparation and the amino acid preparation were selected to compare the effects. (See Table-4)

Similarity with Comparative Example 24: * .P0.5 **: P0.01

Comparative Example 24: 10 ml / Kg of saline solution was prepared.

Comparative Example 25: Saline was added to 1000 mg / Kg of north fish extract and 5 mg / Kg of maltol to prepare 10 ml / Kg.

Comparative Example 26: Physiological saline was added to 1000 mg / Kg of north fish extract and 5 mg / Kg of maltol, 15 mg / Kg of niacin, 25 mg / Kg of vitamin B ₁ , 300 mg / Kg of vitamin C, 50 mg / Kg of sodium aspartate, and 100 mg / Kg of sodium glutamate. 10 ml / Kg was prepared.

As can be seen from the effect test when adding the nutritional supplement of Table 4, niacin, vitamin B ₁ , vitamin C and vitamin C as a nutritional supplement in a solution containing the concentration of northwest extract and maltol which showed the most effective alcohol metabolism effect When the addition of sodium aspartate and sodium glutamate among amino acids (Comparative Example 26) showed a similar effect to that of feed mixed with Northfish X and Maltol (Comparative Example 25). Therefore, it can be seen that it is recommended to add one or two or more nutritional supplements of vitamins, amino acids to provide nutrition to the combination of the two components, the alcohol metabolic effect is proven.

Experimental Example 3 Measurement of Changes in Lipid Peroxide Content in Hepatic Tissues

After fasting the SD-based rats for 18 hours, one group of 6 rats was mixed with physiological saline and the following experimental drugs were administered orally at a volume of 10 mg / Kg. After 6 hours, the liver was extracted and the lipid peroxide content in the tissue lysate was measured (Alcohol and Alcoholism 1988.24 (6). 519-524). The results are shown in Table-5.

Significance with Positive Control: *: P0.05 **: P 0.01

Negative control group: 10ml / kg saline solution was prepared.

Positive Control: Ethanol was prepared at 5 g / kg.

Comparative Example 27: The physiological saline was added to 1000ml / kg of the bookfish extract was prepared at 10ml / kg.

Comparative Example 28: 10 ml / kg was prepared by adding physiological saline to maltol 5 mg / kg.

Comparative Example 29: Niacin 15 mg / kg, vitamin B ₁ 25 mg / kg, vitamin C 300 mg / kg, sodium aspartate 50 mg / kg, sodium glutamate 10 mg / kg was added to prepare a 10 ml / kg.

Comparative Example 30: Physiological saline was added to 1000 mg / kg of Northfish extract and 5 mg / kg of maltol to prepare 10 ml / kg.

Comparative Example 31: Pollack x 1000mg / kg, maltol 5mg / kg, Niacin 15ml / kg, vitamin B ₁ 25mg / kg, Vitamin C 300mg / kg, aspartic acid sodium 50mg / kg, glutamic 10ml was added to physiological saline in sodium 10mg / kg / kg was prepared.

From the results of the experiment in Table 5, the combination of north fish extract and maltol is significantly inhibited the formation of lipid peroxides caused by alcohol intake, which is also effective in preventing alcoholic liver, and also niacin And vitamins and amino acid groups added when the effect is even higher.

In the case of bookfish extract, the maximum effect was obtained from 1000mg / kg or more from the above various experimental examples, and in case of maltol, the maximum effect appeared from 5mg / kg or more, and in the combined effect of bookfish extract and maltol, When the maltol 5mg / kg-25mg / kg blended with respect to X 1000mg / kg, that is, the weight ratio was based on the maltol based on the concentration of the north fish extract 1:40 to 1: 200 showed an excellent effect. In addition, since the formation of lipid peroxides induced by alcohol intake was significantly inhibited in terms of protecting against liver damage, it was confirmed that the effect on the prevention of liver disease caused by the large intake of alcohol was shown.

In order to be able to take the North Korean extract and maltol, whose effectiveness has been demonstrated, it can be prescribed and exemplified, and in this case, the rate of drug metabolism of the rat used in the above experimental example is 8-10 times higher than that of a normal human (60Kg). Based on the fact that it is fast, the amount of North Ax and Maltol administered in the Experimental Example can be prescribed accordingly. The 500 mg / kg and 3000mg / kg of Northwest Ax in the Experimental Example are 3.0g-3.75g / 60g and 18.0g- 22.5g / 60kg and maltol 5mg / kg and 25mg / kg are converted to 30mg-37.5mg / 60Kg and 150mg-187.5mg / 60kg respectively. Therefore, the single dose of the food composition of the present invention is summarized as containing 3.0 g-22.5 g of north fish extract and 30-187.5 mg of maltol.

On the other hand, as shown in the stability test results according to the following examples (see Table 6), the composition of the present invention, when the concentration of the bookfish extract per unit liquid is 20% or more as a unique strong odor and precipitate formation, such as liquid Due to the inadequate stability, it was reasonable to set an upper limit of 15% of the effective Northfish extract per single liquid formulation.

Hereinafter, the preparation examples of the food composition of the present invention are described in Examples, Examples 1 to 8 relate to liquids, and Examples 9 to 10 relate to powders.

Example 1

North fish extract 5%, maltol 0.125%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, sodium benzoate 0.05% was added to the purified water 100ml was prepared.

Example 2

North fish extract 10%, maltol 0.100%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, sodium benzoate 0.05% was added to the purified water to prepare a 100ml.

Example 3

North fish extract 15%, maltol 0.075%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, sodium benzoate 0.05% was added to the purified water to prepare a 100ml.

Example 4

North fish extract 20%, maltol 0.100%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, sodium benzoate 0.05% was added to the purified water to prepare a 100ml.

Example 5

Northfish extract 5%, maltol 0.125%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, niacin 0.02%, vitamin B ₁ 0.005%, vitamin C 0.2%, sodium aspartate 0.05%, sodium glutamate 0.02 %, Sodium benzoate 0.05% was added, and purified water was added to prepare 100 ml.

Example 6

North fish extract 10%, maltol 0.100%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, niacin 0.02%, vitamin B ₁ 0.005%, vitamin C 0.2%, sodium aspartate 0.05%, sodium glutamate 0.02 %, Sodium benzoate 0.05% was added, and purified water was added to prepare 100 ml.

Example 7

North fish extract 15%, maltol 0.075%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, niacin 0.02%, vitamin B1 0.005%, vitamin C 0.2%, sodium aspartate 0.05%, sodium glutamate 0.02% , Sodium benzoate 0.05% was added and purified water was added to prepare 100ml.

Example 8

Northwest extract 20%, maltol 0.100%, liquid fructose 10%, honey 1%, citric acid 0.25%, fruit juice 12%, niacin 0.02%, vitamin B ₁ 0.005%, vitamin C 0.2%, sodium aspartate 0.05%, sodium glutamate 0.02 %, Sodium benzoate 0.05% was added, and purified water was added to prepare 100 ml.

Example 9

5 g of northwest extract is adsorbed on a suitable amount of lactose, dried, and then sieved. Maltol 125 mg, niacin 20 mg, vitamin B ₁ 5 mg, vitamin C 100 mg, sodium aspartate 50 mg, sodium glutamate 20 mg, stevioside, masking flavor, and anhydrous silicic acid It was prepared in 7 g units according to the conventional powder preparation method.

Example 10

10 g of north fish extract is adsorbed on an appropriate amount of lactose, dried, and then sieved. A mixture of maltol 100 mg, niacin 20 mg, vitamin B ₁ 5 mg, vitamin C 100 mg, sodium aspartate 50 mg, sodium glutamate 20 mg, stevioside, masking flavor, and light anhydrous silicic acid It was prepared in 25g units according to the conventional powder preparation method.

Experimental Example Stability Test of Composition

In Example 1-10, the discoloration and sedimentation over time were observed in the following test conditions, and the results are shown in Table-6.

[Exam conditions]

-Test material: The solution prepared in Examples 1 to 8 is placed in a colorless transparent glass bottle and capped. Examples 9 and 10 were filled in aluminum stick packaging.

Storage condition: room temperature

Measurement period: Immediately after manufacture, after 1 month, 2 months, 3 months

Test item: discoloration and precipitation

(Consideration) As shown in Table 6 above, after three months of storage at room temperature, precipitates were generated only in the liquid solution containing 20% of the north fish extracts of Examples 4 and 8, but the concentration was significantly changed at the concentration below that. Was not observed, and even in the case of the powder, there was no significant change in the milky white property, and both the liquid formulation and the powder composition of the present invention were judged to be stable.

Claims (1)

1. Food composition for the prevention, removal and liver protection of hangover by alcohol, characterized by containing north fish extract and maltol as an active ingredient and additional nutritional supplements. 2. The composition of claim 1, wherein the nutritional supplement component is selected from vitamins and amino acids. 3. The composition of claim 2, wherein the vitamin agent is selected from niacin, vitamin B ₁ and vitamin C, and the amino acid agent is selected from asparagine and glutamate. 4. Composition according to item 1, characterized in that the formulation of the composition is selected from liquid, powder form. 5. The composition according to item 4, wherein the composition is 3.0g-22.5g of north fish extract and maltol 30mg-187.5mg. 6. Composition according to any one of items 4 to 5, characterized in that the concentration of Northfish extract in the composition is 5-15%. 7. The composition according to any one of items 1 to 4, wherein the composition has a mixed weight ratio of maltol and north fish extract from 1:40 to 1: 200.