JPWO2021200299A5 - - Google Patents
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- JPWO2021200299A5 JPWO2021200299A5 JP2022511936A JP2022511936A JPWO2021200299A5 JP WO2021200299 A5 JPWO2021200299 A5 JP WO2021200299A5 JP 2022511936 A JP2022511936 A JP 2022511936A JP 2022511936 A JP2022511936 A JP 2022511936A JP WO2021200299 A5 JPWO2021200299 A5 JP WO2021200299A5
- Authority
- JP
- Japan
- Prior art keywords
- biological tissue
- tissue repair
- action
- extracellular vesicles
- promoting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 210000004027 cell Anatomy 0.000 claims 16
- 230000002401 inhibitory effect Effects 0.000 claims 12
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims 12
- 230000017423 tissue regeneration Effects 0.000 claims 12
- 230000009758 senescence Effects 0.000 claims 11
- 210000001519 tissue Anatomy 0.000 claims 11
- 238000004519 manufacturing process Methods 0.000 claims 10
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 claims 9
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 claims 9
- 239000000126 substance Substances 0.000 claims 9
- 230000032677 cell aging Effects 0.000 claims 8
- 239000003112 inhibitor Substances 0.000 claims 8
- 102000004127 Cytokines Human genes 0.000 claims 7
- 108090000695 Cytokines Proteins 0.000 claims 7
- 230000002757 inflammatory effect Effects 0.000 claims 7
- 238000000034 method Methods 0.000 claims 7
- 230000015556 catabolic process Effects 0.000 claims 6
- 238000006731 degradation reaction Methods 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 239000003795 chemical substances by application Substances 0.000 claims 4
- 230000005764 inhibitory process Effects 0.000 claims 4
- 230000001737 promoting effect Effects 0.000 claims 4
- 108010014258 Elastin Proteins 0.000 claims 3
- 210000003491 skin Anatomy 0.000 claims 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 2
- 101100369802 Caenorhabditis elegans tim-1 gene Proteins 0.000 claims 2
- 102000016942 Elastin Human genes 0.000 claims 2
- 101150046249 Havcr2 gene Proteins 0.000 claims 2
- 102100034458 Hepatitis A virus cellular receptor 2 Human genes 0.000 claims 2
- 102000008070 Interferon-gamma Human genes 0.000 claims 2
- 108010074328 Interferon-gamma Proteins 0.000 claims 2
- 102000000589 Interleukin-1 Human genes 0.000 claims 2
- 108010002352 Interleukin-1 Proteins 0.000 claims 2
- 102000013462 Interleukin-12 Human genes 0.000 claims 2
- 108010065805 Interleukin-12 Proteins 0.000 claims 2
- 102000003810 Interleukin-18 Human genes 0.000 claims 2
- 108090000171 Interleukin-18 Proteins 0.000 claims 2
- 102000004889 Interleukin-6 Human genes 0.000 claims 2
- 108090001005 Interleukin-6 Proteins 0.000 claims 2
- 102000004890 Interleukin-8 Human genes 0.000 claims 2
- 108090001007 Interleukin-8 Proteins 0.000 claims 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 2
- 239000004480 active ingredient Substances 0.000 claims 2
- 210000001691 amnion Anatomy 0.000 claims 2
- 210000001185 bone marrow Anatomy 0.000 claims 2
- 210000003074 dental pulp Anatomy 0.000 claims 2
- 229920002549 elastin Polymers 0.000 claims 2
- 210000004700 fetal blood Anatomy 0.000 claims 2
- 230000014509 gene expression Effects 0.000 claims 2
- 229920002674 hyaluronan Polymers 0.000 claims 2
- 229960003160 hyaluronic acid Drugs 0.000 claims 2
- 229960003130 interferon gamma Drugs 0.000 claims 2
- 229940117681 interleukin-12 Drugs 0.000 claims 2
- 229940100601 interleukin-6 Drugs 0.000 claims 2
- 229940096397 interleukin-8 Drugs 0.000 claims 2
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 claims 2
- 210000003205 muscle Anatomy 0.000 claims 2
- 210000005036 nerve Anatomy 0.000 claims 2
- 210000002826 placenta Anatomy 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 210000003954 umbilical cord Anatomy 0.000 claims 2
- 102000008186 Collagen Human genes 0.000 claims 1
- 108010035532 Collagen Proteins 0.000 claims 1
- 230000010261 cell growth Effects 0.000 claims 1
- 230000004663 cell proliferation Effects 0.000 claims 1
- 229920001436 collagen Polymers 0.000 claims 1
- 230000037319 collagen production Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 108700026220 vif Genes Proteins 0.000 claims 1
Claims (18)
細胞外小胞が炎症性サイトカインにより刺激を加えた間葉系幹細胞に由来するものである、又は／及び細胞外小胞がホスファチジルセリンに対して親和性を有する物質を利用する方法により得られるものである、細胞老化抑制剤又は生体組織修復促進剤。 A cell aging inhibitor or biological tissue repair promoter containing extracellular vesicles derived from mesenchymal stem cells as an active ingredient,
The extracellular vesicles are derived from mesenchymal stem cells stimulated with inflammatory cytokines, or/and the extracellular vesicles are obtained by a method using a substance having affinity for phosphatidylserine. A cell aging inhibitor or biological tissue repair promoter.
細胞外小胞が、炎症性サイトカインにより刺激を加えた間葉系幹細胞に由来するものである、又は／及び細胞外小胞がホスファチジルセリンに対して親和性を有する物質を利用する方法により得られるものであり、且つ
遺伝子が、細胞増殖抑制遺伝子、コラーゲン遺伝子、コラーゲン分解酵素遺伝子、ヒアルロン酸合成酵素遺伝子、ヒアルロン酸分解酵素遺伝子、エラスチン遺伝子、及びエラスチン分解酵素遺伝子から選ばれる少なくとも１種の遺伝子である、遺伝子発現調節剤。 A gene expression regulator containing extracellular vesicles derived from mesenchymal stem cells as an active ingredient,
Extracellular vesicles are derived from mesenchymal stem cells stimulated with inflammatory cytokines, or/and extracellular vesicles are obtained by a method using a substance having affinity for phosphatidylserine and the gene is at least one gene selected from a cell growth inhibitory gene, a collagen gene, a collagenase gene, a hyaluronic acid synthase gene, a hyaluronanase gene, an elastin gene, and an elastinase gene. A gene expression regulator.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020065052 | 2020-03-31 | ||
| JP2020065052 | 2020-03-31 | ||
| PCT/JP2021/011546 WO2021200299A1 (en) | 2020-03-31 | 2021-03-19 | Cellular aging inhibitor, biological tissue repair promoter, gene expression regulator, and manufacturing method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2021200299A1 JPWO2021200299A1 (en) | 2021-10-07 |
| JPWO2021200299A5 true JPWO2021200299A5 (en) | 2022-11-15 |
| JP7478227B2 JP7478227B2 (en) | 2024-05-02 |
Family
ID=77928040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022511936A Active JP7478227B2 (en) | 2020-03-31 | 2021-03-19 | Cell aging inhibitor, biological tissue repair promoter, gene expression regulator, and manufacturing method |
Country Status (3)
| Country | Link |
|---|---|
| JP (1) | JP7478227B2 (en) |
| KR (1) | KR20220148883A (en) |
| WO (1) | WO2021200299A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7371975B1 (en) | 2022-09-27 | 2023-10-31 | セルソース株式会社 | Method for producing extracellular vesicles and composition containing extracellular vesicles |
| JP7433685B1 (en) | 2023-06-09 | 2024-02-20 | 日生バイオ株式会社 | Dermal fibroblast function enhancing agent and cosmetics containing the same |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102632022B1 (en) | 2014-12-05 | 2024-01-31 | 후지필름 가부시키가이샤 | Tim protein-bound carrier, methods for obtaining, removing and detecting extracellular membrane vesicles and viruses using said carrier, and kit including said carrier |
| CN108392494A (en) * | 2018-05-15 | 2018-08-14 | 江苏大学 | Applications of the exosome of people's umbilical cord mesenchymal stem cells secretion in preparing the drug for repairing skin ultraviolet injury |
| CN109172859A (en) * | 2018-09-06 | 2019-01-11 | 上海长海医院 | Human stem cell source excretion bluk recombination exogenous hyaluronic acid is preparing the application in skin wound defect repair drug or material |
| CN110548001A (en) | 2019-09-06 | 2019-12-10 | 沈阳细胞治疗工程技术研发中心有限公司 | Repair anti-aging skin care product containing umbilical cord mesenchymal stem cell exosomes |
-
2021
- 2021-03-19 JP JP2022511936A patent/JP7478227B2/en active Active
- 2021-03-19 WO PCT/JP2021/011546 patent/WO2021200299A1/en active Application Filing
- 2021-03-19 KR KR1020227034047A patent/KR20220148883A/en unknown
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