JPWO2020235668A5 - - Google Patents
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- JPWO2020235668A5 JPWO2020235668A5 JP2021520870A JP2021520870A JPWO2020235668A5 JP WO2020235668 A5 JPWO2020235668 A5 JP WO2020235668A5 JP 2021520870 A JP2021520870 A JP 2021520870A JP 2021520870 A JP2021520870 A JP 2021520870A JP WO2020235668 A5 JPWO2020235668 A5 JP WO2020235668A5
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- JP
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- area
- screening
- comparison
- emulsion
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- 238000012216 screening Methods 0.000 description 6
- 239000013068 control sample Substances 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
Description
ステップS41において、スクリーニング試料の染色部分の面積Atと、スクリーニング対照試料の染色部分の面積Acとを比較する。
面積の比較は、面積の絶対値の比較であっても良く、スクリーニング対照試料の面積を100%とした場合における面積の割合の比較であってもよい。
複数のスクリーニング試料について評価を行う場合には、スクリーニング対照試料の面積を100%とした比較を行うのが好ましい。
In step S41, the area At of the stained portion of the screening sample and the area Ac of the stained portion of the screening control sample are compared.
The area comparison may be a comparison of the absolute value of the area, or may be a comparison of the ratio of the area when the area of the screening control sample is 100%.
When evaluating a plurality of screening samples, it is preferable to make a comparison with the area of the screening control sample as 100%.
一方で、面積Atが面積Acと同一、又は面積Atが面積Acより大きい高い場合(面積At ≧ 面積Ac)にはステップS43に移行し、当該スクリーニング試料に添加した探索対象成分は、接着タンパク質の量を減少する作用を有さない成分であると評価する。 On the other hand, when the area At is the same as the area Ac or the area At is higher than the area Ac (area At ≧ area Ac), the process proceeds to step S43, and the search target component added to the screening sample is the adhesive protein. It is evaluated as a component that does not have the effect of reducing the amount.
(2)被験者及び測定部位
本実施例では、40歳から52歳の女性20名を被験者とした。
そして、本実施例では、被験者顔面における、シミが存在する部位(92箇所)を測定部位として選定した。
各被験者のシミが存在する部位に、製造例2の乳液を1日2回塗布し、これを12週間続けた。
(2) Subjects and measurement sites In this example, 20 women aged 40 to 52 years were used as subjects.
Then, in this embodiment, the sites (92 sites) where the stains are present on the subject's face were selected as the measurement sites.
The emulsion of Production Example 2 was applied to the spots of each subject twice a day, and this was continued for 12 weeks.
解析用画像全体の面積を100%として、蛍光部分の面積を算出した。結果を表5、表6、及び図17(代表)、図18、19に示す。なお、乳液使用12週間経過後のデータは、シミ部位92箇所のうち、80箇所のデータを集計し、同箇所における乳液使用前のデータと比較した。また、図18において、**はp<0.01であり、図19において、***はp<0.001である。 The area of the fluorescent portion was calculated with the area of the entire analysis image as 100%. The results are shown in Tables 5, 6, and 17 (representative), 18 and 19. As for the data after 12 weeks of using the emulsion, the data of 80 of the 92 spots were aggregated and compared with the data before using the emulsion at the same site. Further, in FIG. 18, ** is p <0.01, and in FIG. 19, *** is p <0.001.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019096940 | 2019-05-23 | ||
JP2019096940 | 2019-05-23 | ||
PCT/JP2020/020229 WO2020235668A1 (en) | 2019-05-23 | 2020-05-22 | Method for screening component that affects fluctuation of adhesion proteins in stratum corneum cells |
Publications (3)
Publication Number | Publication Date |
---|---|
JPWO2020235668A1 JPWO2020235668A1 (en) | 2020-11-26 |
JPWO2020235668A5 true JPWO2020235668A5 (en) | 2022-02-18 |
JP7513287B2 JP7513287B2 (en) | 2024-07-09 |
Family
ID=73458890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2021520870A Active JP7513287B2 (en) | 2019-05-23 | 2020-05-22 | Screening method for components that affect the increase or decrease of adhesion proteins between stratum corneum cells |
Country Status (5)
Country | Link |
---|---|
JP (1) | JP7513287B2 (en) |
CN (1) | CN113874704A (en) |
SG (1) | SG11202112996PA (en) |
TW (1) | TW202101001A (en) |
WO (1) | WO2020235668A1 (en) |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3179887B2 (en) * | 1992-09-02 | 2001-06-25 | ポーラ化成工業株式会社 | Evaluation method of skin melanin |
JP3453871B2 (en) * | 1994-08-31 | 2003-10-06 | 株式会社資生堂 | Evaluation method of desmosome function |
JP2001097815A (en) | 1999-09-28 | 2001-04-10 | Shiseido Co Ltd | W/o-type emulsion composition and emulsified cosmetic |
JP3618093B2 (en) * | 2001-10-29 | 2005-02-09 | ポーラ化成工業株式会社 | Keratinocyte staining method and keratinocyte preparation method |
JP2004051596A (en) * | 2002-07-24 | 2004-02-19 | Noevir Co Ltd | Agent for promoting biosynthesis of epidermic transglutaminase and activity promoting agent |
US20060216756A1 (en) * | 2003-04-21 | 2006-09-28 | Shiseido Company Ltd. | Method for evaluating oxidized protein in horny cell layer |
US20080039940A1 (en) * | 2004-03-11 | 2008-02-14 | Kouji Hashimoto | Biological Tissue Sheet, Method Of Forming The Same And Transplantation Method By Using The Sheet |
JP2006017688A (en) * | 2004-06-01 | 2006-01-19 | Pola Chem Ind Inc | Differentiation method for subcorneal cell by autofluorescence |
DK1800129T3 (en) * | 2004-09-23 | 2013-07-01 | Tripath Imaging Inc | Polycationic polymer coatings for immobilization of biological samples |
CN101622538B (en) * | 2007-02-14 | 2012-09-26 | 宝丽化学工业有限公司 | Method of supporting the differentiation of horny layer cells |
JP5065237B2 (en) * | 2008-12-26 | 2012-10-31 | 常盤薬品工業株式会社 | Noninvasive evaluation method of local pathology of atopic dermatitis |
US20110262025A1 (en) * | 2010-02-05 | 2011-10-27 | Bradley Bryan Jarrold | Cosmetic Compositions and Methods for Maintaining and Improving Barrier Function of the Stratum Corneum and to Reduce the Visible Signs of Aging in Skin |
US20130102486A1 (en) * | 2011-10-20 | 2013-04-25 | The Board Of Trustees Of The Leland Stanford Junior University | Perp as a prognostic and diagnostic marker for dysplasia and cancer |
JP6243123B2 (en) * | 2013-01-21 | 2017-12-06 | 株式会社 資生堂 | Method for evaluating stratum corneum state and method for evaluating stratum corneum improvement effect of cosmetics |
CN105143847B (en) * | 2013-02-05 | 2019-05-28 | 三路影像公司 | Cell dyeing composition and application thereof |
JP6580895B2 (en) | 2014-08-08 | 2019-09-25 | 株式会社コーセー | Astaxanthin-containing desmoglein reducing agent |
JP6738280B2 (en) * | 2014-10-24 | 2020-08-12 | 株式会社 資生堂 | Cosmetic method and evaluation method for improving skin condition resulting from suppression or enhancement of stratum corneum peeling |
JP6612137B2 (en) * | 2015-02-20 | 2019-11-27 | 御木本製薬株式会社 | Method for preparing stratum corneum cell specimen |
JP6863548B2 (en) | 2015-08-11 | 2021-04-21 | ポーラ化成工業株式会社 | Cosmetic base for improving skin condition and its screening method |
CA3016914C (en) * | 2016-03-15 | 2019-08-27 | Laboratory Corporation Of America Holdings | Methods of assessing protein interactions between cells |
-
2020
- 2020-05-22 SG SG11202112996PA patent/SG11202112996PA/en unknown
- 2020-05-22 WO PCT/JP2020/020229 patent/WO2020235668A1/en active Application Filing
- 2020-05-22 CN CN202080037961.3A patent/CN113874704A/en active Pending
- 2020-05-22 JP JP2021520870A patent/JP7513287B2/en active Active
- 2020-05-22 TW TW109117065A patent/TW202101001A/en unknown
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