JPWO2020223515A5 - - Google Patents

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JPWO2020223515A5
JPWO2020223515A5 JP2021564239A JP2021564239A JPWO2020223515A5 JP WO2020223515 A5 JPWO2020223515 A5 JP WO2020223515A5 JP 2021564239 A JP2021564239 A JP 2021564239A JP 2021564239 A JP2021564239 A JP 2021564239A JP WO2020223515 A5 JPWO2020223515 A5 JP WO2020223515A5
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score
varicocele
cap
acrosome
subject
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JP2021564239A
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JP2022531174A (en
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Priority claimed from PCT/US2020/030783 external-priority patent/WO2020223515A1/en
Publication of JP2022531174A publication Critical patent/JP2022531174A/en
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精索静脈瘤に罹患している対象における生殖アプローチを特定するための方法であって、
（ａ）前記対象から得た精子サンプルを固定剤で固定することと、
（ｂ）前記精子サンプルを、ＧＭ１局在パターンを特定する標識で標識することと、
（ｃ）前記精子サンプルで発現されるＧＭ１局在パターンの総数（「総ＧＭ１」）、前記精子サンプルで発現される頂端先体（ＡＡ）のＧＭ１局在パターンの数及び先体原形質膜（ＡＰＭ）のＧＭ１局在パターンの数を測定することと、
（ｄ）前記ＡＡのＧ Ｍ１ 局在パターン数と前記ＡＰＭのＧ Ｍ１ 局在パターンの数との合計を、Ｇ Ｍ１ 標識局在パターンの総数で除した比として定義されるＣＡＰ－ＳｃｏｒｅＴＭを得ることと、
（ｅ）推奨される生殖アプローチを使用するための指示を与えることと
を含むことを特徴とする方法。 A method for identifying a reproductive approach in a subject suffering from varicocele comprising :
(a ) fixing a sperm sample obtained from the subject with a fixative;
( b ) labeling the sperm sample with a label that specifies a GM1 localization pattern;
( c ) the total number of G M1 localization patterns expressed in the sperm sample (“total G M1 ”), the number of apical acrosome (AA) G M1 localization patterns expressed in the sperm sample and the acrosome measuring the number of G M1 localization patterns in the plasma membrane (APM);
( d ) Obtain a CAP-Score TM defined as the ratio of the sum of the number of G M1 localization patterns in the AA and the number of G M1 localization patterns in the APM divided by the total number of G M1 labeled localization patterns. and
( e ) providing instructions for using the recommended reproductive approach. 前記対象が精索静脈瘤と診断された場合、更に前記精索静脈瘤が、理学的検査によって診断される、請求項１に記載の方法。 2. The method of claim 1 , wherein if the subject is diagnosed with varicocele, the varicocele is further diagnosed by physical examination. 前記生殖アプローチが、精索静脈瘤修復手術、自然受胎、子宮内授精（ＩＵＩ）、体外受精（ＩＶＦ）、子宮頸管内授精（ＩＣＩ）、顕微授精（ＩＣＳＩ）、配偶子卵管内移植（ＧＩＦＴ）、及び囲卵腔内精子注入（ＳＵＺＩ）から選択される、請求項１に記載の方法。 The reproductive approaches include varicocele repair surgery, natural conception, intrauterine insemination (IUI), in vitro fertilization (IVF), intracervical insemination (ICI), microinsemination (ICSI), gamete intrafallopian transfer (GIFT) , and perivitelline sperm injection (SUZI). 前記生殖アプローチが、前記対象の前記ＣＡＰ－ＳｃｏｒｅＴＭが参照集団平均未満で１標準偏差より高い場合、自然受胎、ＩＵＩ、及びＩＣＩから選択される、請求項１に記載の方法。 2. The method of claim 1, wherein the reproductive approach is selected from spontaneous conception, IUI, and ICI when the subject's CAP-Score TM is less than the reference population mean and greater than one standard deviation. 前記生殖アプローチが、前記ＣＡＰ－ＳｃｏｒｅＴＭが参照集団平均未満で１標準偏差より低い場合、ＩＶＦ、ＩＣＳＩ、ＧＩＦＴ、及びＳＵＺＩから選択される、請求項１に記載の方法。 2. The method of claim 1, wherein said reproductive approach is selected from IVF, ICSI, GIFT, and SUZI when said CAP-Score TM is less than one standard deviation below the reference population mean. 前記指示が、前記対象に与えられる、請求項１に記載の方法。 2. The method of claim 1, wherein said instruction is provided to said subject. 前記指示が、医療専門家に与えられる、請求項１に記載の方法。 2. The method of claim 1, wherein the instructions are provided to a medical professional. 前記生殖アプローチが、精索静脈瘤修復手術である、請求項１に記載の方法。 2. The method of claim 1, wherein the reproductive approach is varicocele repair surgery. 前記精索静脈瘤修復手術が成功したかどうかを判定するために、精索静脈瘤修復手術後に得られた精子サンプルに手術後のＣＡＰ－ＳｃｏｒｅＴＭを提供することを更に含む、請求項８に記載の方法。 9. The method of claim 8, further comprising providing a sperm sample obtained after varicocele repair surgery with a post-operative CAP-Score ™ to determine whether the varicocele repair surgery was successful. described method. 前記ＣＡＰ－ＳｃｏｒｅＴＭを前記手術後のＣＡＰ－ＳｃｏｒｅＴＭと比較することを更に含み、前記手術後のＣＡＰ－ＳｃｏｒｅＴＭの少なくとも７パーセントの上昇が、精索静脈瘤修復手術の成功を示す、請求項９に記載の方法。 further comprising comparing said CAP-Score TM to said post-operative CAP-Score TM , wherein an increase of at least 7 percent in said post-operative CAP-Score TM indicates successful varicocele repair surgery. Item 9. The method of Item 9. 前記ＧＭ１局在パターンが、頂端先体（ＡＡ）、先体原形質膜（ＡＰＭ）、裏打ち細胞、中間体（ＩＮＴＥＲ）、先体後原形質膜（ＰＡＰＭ）、エクアトリアルセグメント（ＥＳ）、及びディフューズ（ＤＩＦＦ）から選択される、請求項１に記載の方法。 The G M1 localization pattern includes apical acrosome (AA), acrosome plasma membrane (APM), lining cell, intermediate (INTER), post-acrosome plasma membrane (PAPM), equatorial segment (ES), and 2. The method of claim 1, selected from diffuse (DIFF). 前記精子サンプルが、少なくとも３０分間固定される、請求項１に記載の方法。 2. The method of claim 1, wherein said sperm sample is fixed for at least 30 minutes. 前記標識が、蛍光標識である、請求項１に記載の方法。 2. The method of claim 1, wherein said label is a fluorescent label. 前記標識が、コレラ毒素ｂサブユニットである、請求項１３に記載の方法。 14. The method of claim 13, wherein the label is cholera toxin b subunit. 運動性、形態、量、濃度、ｐＨ、粘度、及びそれらの組合せから選択される１以上の精子の特徴を分析することを更に含む、請求項１に記載の方法。 2. The method of claim 1, further comprising analyzing one or more sperm characteristics selected from motility, morphology, quantity, concentration, pH, viscosity, and combinations thereof.
JP2021564239A 2019-04-30 2020-04-30 Use of CAP-SCORE ™ in identifying reproductive approaches in men with varicocele Pending JP2022531174A (en)

Applications Claiming Priority (3)

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US201962840846P 2019-04-30 2019-04-30
US62/840,846 2019-04-30
PCT/US2020/030783 WO2020223515A1 (en) 2019-04-30 2020-04-30 Use of cap-score tm in identification of a reproductive approach in men suffering from varicocele

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JP2022531174A JP2022531174A (en) 2022-07-06
JPWO2020223515A5 true JPWO2020223515A5 (en) 2023-05-10

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US (1) US20220221466A1 (en)
EP (1) EP3958756A4 (en)
JP (1) JP2022531174A (en)
KR (1) KR20220005003A (en)
CN (1) CN114072082A (en)
AU (1) AU2020266846A1 (en)
BR (1) BR112021021574A2 (en)
CA (1) CA3138291A1 (en)
IL (1) IL287556A (en)
MX (1) MX2021013195A (en)
WO (1) WO2020223515A1 (en)

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US8367313B2 (en) * 2003-07-23 2013-02-05 Cornell University Method of determining sperm capacitation
AU2015317864B2 (en) * 2014-09-17 2021-07-01 Cornell University Identifying status of male fertility by determining sperm capacitation
HUE056953T2 (en) * 2015-12-23 2022-03-28 Androvia Life Sciences LLC A method to identify an approach for achieving mammalian fertilization and time period for insemination
WO2017143157A1 (en) * 2016-02-17 2017-08-24 Androvia Life Sciences LLC Methods and test kits for determining male fertility status

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