JPWO2020179053A1 - Liquid transfer cartridge for temperature control device - Google Patents

Abstract

Provided is a high-speed and highly accurate liquid feed cartridge for a temperature control device, which suppresses deterioration of contact thermal resistance due to thermal deformation. The liquid feed cartridge 1 for a temperature control device includes a liquid feed tip 2 that forms a flow path in a gap between a second layer 3 that can be arranged in the temperature control unit 16 and the second layer 3, and a temperature control unit 16. Outside the region of the second layer 3, it is arranged on the upper side which is the side of the liquid feeding tip 2 from the second layer 3 or on the lower side of the second layer 3, is made of an elastic body, and is a strand of the second layer 3. A first layer 4 having a large expansion coefficient is provided.

Description

The present invention relates to a liquid feed cartridge for a temperature control device.

In recent years, genetic testing has come to be used not only for research purposes but also for a wide range of purposes such as personalized medicine and individual identification, and it is desired to shorten not only accuracy but also test time. When conducting a genetic test, high-precision analysis is realized by obtaining a sample containing DNA (Deoxyribonucleic acid, deoxyribonucleic acid), amplifying a small amount of DNA in the sample, and then performing the analysis. Here, as a method for amplifying DNA, the PCR (Polymerase Chain Reaction) method is widely used. In the PCR method, a sample solution containing DNA and a solution containing a reagent for amplifying DNA are mixed, denatured into a single strand at, for example, 94 ° C, and a complementary strand is synthesized at 60 ° C. DNA can be amplified exponentially by repeating these temperature changes. On the other hand, if an excessive temperature rise or temperature drop occurs with respect to the temperature required by the reagent, there arises a problem that the target DNA is not amplified, so that highly accurate temperature control is required.
Furthermore, in order to automate operations such as quantification and stirring of samples and reagents required for genetic testing, the development of a flow path chip that realizes liquid feeding on a disposable substrate is underway, and the elasticity provided on the surface of the flow path chip. Chips that send liquid by utilizing the deformation of the membrane have been reported. Further, in order to realize the PCR method on the liquid feeding chip after the liquid is fed, the development of a structure that gives a temperature change using a Perche element is underway.

On the other hand, when the temperature of the solution is changed via the elastic membrane that controls the liquid feeding provided in the flow path chip, if the elastic membrane and the temperature control device are not in proper contact, the space between the elastic membrane and the temperature control device is reached. As the generated contact heat resistance increases, the temperature difference between the temperature control device and the solution increases, and it becomes difficult to control the temperature to be obtained by the PCR method. If the solution is not controlled to an appropriate temperature, stable DNA amplification cannot be performed, and there is a problem that the reliability of the genetic testing device is lowered.
To solve these problems, a structure for reducing the contact thermal resistance between the temperature control unit and the elastic film has been studied. For example, in Patent Document 1, while applying pressure to the inside of the container unit for accommodating a liquid sample. , A method of heating a liquid sample is disclosed. Further, Patent Document 2 describes a configuration having an upper rigid substrate, a membrane layer, a pedestal substrate, and at least one support cup substrate having a recessed portion having an opening formed therein.

WO2012 / 086168 Gazette Japanese Unexamined Patent Publication No. 2011-30522

In Patent Document 1, by applying pressure to the solution sealed with the membrane and the substrate, the solution is pressed against the temperature control block through the membrane, and the heat exchange efficiency with the temperature control block is enhanced. However, the temperature control structure described in Patent Document 1 requires a mechanism for applying pressure to the reaction vessel portion for controlling the temperature of the solution, which complicates the liquid feeding mechanism, and the membrane is thermally deformed with a temperature change. Since the contact thermal resistance changes with this thermal deformation, there is a problem that it is difficult to give a uniform and highly accurate temperature change. Further, also in Patent Document 2, the membrane undergoes thermal deformation with the temperature change, and the contact thermal resistance changes with the thermal deformation, so that it is difficult to give a uniform and highly accurate temperature change.

Therefore, the present invention provides a liquid feed cartridge for a temperature control device that suppresses deterioration of contact thermal resistance due to thermal deformation and has high speed and high accuracy.

In order to solve the above problems, the liquid feed cartridge for a temperature control device according to the present invention is a liquid feed cartridge that forms a flow path in a gap between a second layer that can be arranged in a temperature control unit and the second layer. The tip and the outside of the region of the temperature control unit are arranged on the upper side of the liquid feeding tip on the side of the second layer or on the lower side of the second layer, and are made of an elastic body and described above. It is characterized by comprising a first layer having a larger linear expansion coefficient than the second layer.

Further, the liquid feed cartridge for another temperature control device according to the present invention includes a liquid feed tip that forms a flow path in the gap between the second layer that can be arranged in the temperature control unit and the second layer. It is provided with a first layer which is arranged on the lower side opposite to the liquid feeding tip from the second layer, is made of an elastic body, and has a larger linear expansion coefficient than the second layer. It is characterized by.

According to the present invention, it is possible to suppress deterioration of contact thermal resistance due to thermal deformation and to provide a high-speed and highly accurate liquid feed cartridge for a temperature control device.
Issues, configurations and effects other than those described above will be clarified by the following description of the embodiments.

It is a schematic top view of the temperature control apparatus according to Example 1 which concerns on one Example of this invention. It is a schematic side view of the temperature control apparatus according to Example 1. FIG. It is a block diagram of the liquid feed cartridge shown in FIGS. 1 and 2. FIG. 3 is a cross-sectional view taken along the line AA' explaining the structure of the temperature control device in FIG. It is sectional drawing of BB' explaining the structure of the temperature control apparatus in FIG. It is a figure which shows the solution introduction state of the liquid feeding cartridge according to Example 1. FIG. It is a figure which shows the flow path sealed state of the liquid feeding cartridge according to Example 1. FIG. It is a figure which shows the state which held the solution of the liquid feeding cartridge according to Example 1 in a temperature control flow path. It is a figure which shows the moving state of the liquid feed cartridge from the temperature control flow path by Example 1. FIG. It is a figure which shows the solution discharge state of the liquid feeding cartridge according to Example 1. FIG. It is sectional drawing explaining the structure of the temperature control apparatus by Example 2 which concerns on another Example of this invention. It is a figure which shows the modification of FIG. 7. It is sectional drawing explaining the structure of the temperature control apparatus according to Example 3 which concerns on another Example of this invention. It is sectional drawing explaining the structure of the temperature control apparatus by Example 4 which concerns on another Example of this invention. It is sectional drawing explaining the structure of the temperature control apparatus according to Example 5 which concerns on another Example of this invention.

The "chemical analyzer" to which the liquid feed cartridge of the present invention is applied includes a DNA analyzer, a nucleic acid amplification device, and a genetic test device.
Hereinafter, examples of the present invention will be described with reference to the drawings.

FIG. 1 is a schematic top view of the temperature control device 20 according to the first embodiment of the present invention. Further, FIG. 2 is a schematic side view of the temperature control device 20. In FIG. 1, the shape of the flow path formed by the liquid feed cartridge 1 and the liquid feed control unit 5 is shown by a broken line. As shown in FIG. 2, the temperature control device 20 includes a liquid feed cartridge 1, a liquid feed control unit 5, and a temperature control unit 16. FIG. 3 shows the structure of each layer constituting the liquid feeding cartridge 1. The liquid feed cartridge 1 is composed of a first layer 4 made of a liquid feed tip 2 and an elastic body, and a second layer 3 made of a material having a coefficient of linear expansion smaller than that of the first layer, and is composed of a liquid feed control unit 5. It is fixed to the upper surface of. The liquid feed cartridge 1 is removable from the upper surface of the liquid feed control unit 5. The liquid feeding tip 2 is formed with an introduction hole 10 for introducing a solution and a discharge hole 11 for discharging the solution. A groove is formed on the bottom surface of the liquid feeding tip 2 to form a flow path 8 and a temperature control flow path 8a. The introduction hole 10 and the discharge hole 11 communicate with each other from the upper surface to the lower surface of the liquid feeding tip 2, and the solution can be moved through the flow path 8 and the temperature control flow path 8a. The second layer 3 has an opening 3a having substantially the same shape as the first liquid feed valve 9a, the second liquid feed valve 9b, and the third liquid feed valve 9c formed in the liquid feed control unit 5. The first layer 4 has an opening 4a having substantially the same shape as the temperature control flow path 8a. The liquid feed control unit 5 is formed with an opening 9d for fixing the temperature control unit 16 and recesses serving as a first liquid feed valve 9a, a second liquid feed valve 9b, and a third liquid feed valve 9c. .. Further, through holes 12 are formed on the bottom surfaces of the first liquid feeding valve 9a, the second liquid feeding valve 9b, and the third liquid feeding valve 9c. Further, the through hole 12 is connected to a device (not shown) that controls the introduction and discharge of a fluid such as air. FIG. 4 shows a cross-sectional view of the temperature control device 20 shown in FIG. 1 in the AA'direction. The liquid feed control unit 5 and the temperature control unit 16 are fixed to the bottom surface of the liquid feed cartridge 1. Here, the temperature control block 6 constituting the temperature control unit 16 contacts the second layer 3 through the opening 4a (FIG. 3) formed in the first layer 4, and the temperature control of the liquid feeding tip 2 is performed. It is fixed to the bottom surface of the flow path 8a. The first layer 4 formed in the liquid feed control unit 5 does not come into contact with the temperature control block 6. The second layer 3 has an opening 3a that is substantially the same as the first liquid feeding valve 9a, the second liquid feeding valve 9b, and the third liquid feeding valve 9c, and the opening 3a and the liquid feeding valve. Paste them so that the positions of are the same. Therefore, the second layer 3 is not formed on the upper portion of the opening of the liquid feed valve. FIG. 5 shows a cross-sectional view of the temperature control device 20 in the BB'direction. A second layer 3 is arranged on the bottom surface of the temperature control flow path 8a formed in the liquid feeding tip 2, and the temperature is pressed by the temperature control block 6 through the opening 4a formed in the first layer 4. The bottom surface of the tuning channel 8a is sealed.

Next, an example of liquid transfer using the liquid feed control unit 5 in the temperature control device 20 will be shown. First, as shown in FIG. 6A, the solution 13 is arranged in the introduction hole 10 of the liquid feeding tip 2 (solution introduction state). Here, as shown in FIG. 6B, air is introduced into the second liquid feed valve 9b through the through hole 12, and the first layer 4 is pushed up, that is, the second liquid feed valve 9b is pushed up, and the second liquid feed valve 9b is pushed up. The flow path 8 is sealed by bringing the layer 4 of 1 into contact with the bottom surface of the liquid feeding tip 2 (flow path sealed state). Here, the first layer 4 is deformed by discharging the air of the first liquid feeding valve 9a, that is, by pulling down the first liquid feeding valve 9a, the solution 13 arranged in the introduction hole 10 flows through the flow path. It is sucked into the first liquid feeding valve 9a through 8. Further, as shown in FIG. 6C, first the third liquid feed valve 9c is pushed up to seal the flow path 8, and then the second liquid feed valve 9b is pulled down to transfer the solution 13 to the temperature control flow path 8a in the liquid feed tip 2. The first liquid feed valve 9a is pushed up to seal the flow path 8, the third liquid feed valve 9c is pulled down to open the flow path 8, and the second liquid feed valve 9b is pushed up to make a solution. 13 is held in the temperature control flow path 8a (a state in which the solution is held in the temperature control flow path). Here, the temperature of the temperature control block 6 can be changed by the temperature control element 7 constituting the temperature control unit 16, and the temperature can be changed to the solution 13 via the second layer 3. After the temperature change operation is completed, as shown in FIG. 6D, the third liquid feed valve 9c is pushed up to seal the flow path 8, and the first liquid feed valve 9a and the third liquid feed valve 9c are pulled down. The solution 13 advances to the region of the second liquid feed valve 9b (moving state of the solution from the temperature control flow path). Further, as shown in FIG. 6E, the third liquid feed valve 9c is pulled down to open the flow path 8, and the first liquid feed valve 9a and the second liquid feed valve 9b are pushed up to discharge the solution 13 into the discharge hole 11. The temperature-treated solution 13 can be taken out from the liquid feed cartridge 1 (solution discharge state).

Here, by installing a temperature sensor (not shown) required for controlling the temperature by the temperature control element 7 in the temperature control block 6, it is possible to confirm that the temperature change is desired. For example, a sample containing DNA and a reagent containing an enzyme that amplifies DNA are mixed, and the liquid is sent to the temperature control flow path 8a on the liquid feed cartridge 1, and the temperature is repeatedly changed to 94 ° C and 60 ° C. By doing so, the DNA can be amplified and analyzed.

According to the above-described embodiment, the thermal deformation of the bottom surface forming the temperature control flow path 8a is small, and the contact thermal resistance between the temperature control block 6 and the second layer 3 is kept uniform. Therefore, the temperature change due to the temperature control unit 16 can be accurately transmitted to the solution 13. According to this embodiment, the temperature control block 6 is in contact with only the second layer 3 having a small thermal deformation and not in contact with the first layer 4 having a large thermal deformation. Since deformation is suppressed, a uniform contact state is maintained and the contact thermal resistance does not change.

In this embodiment, the flow path 8 is formed in only one system in the liquid feeding tip 2, but the flow path 8 is not limited to one system, and the introduction hole and the flow path are used to feed and mix a plurality of solutions. , A liquid feeding tip having a plurality of discharge holes may be used. Further, since the temperature control block 6 is intended to efficiently transmit the temperature change of the temperature control element 7 to the solution 13 held in the temperature control flow path 8a, it is preferably a metal material having high thermal conductivity. , For example, preferably made of aluminum or copper. Further, the temperature control element 7 can have a structure in which the temperature is adjusted by passing through a heating / cooling medium using a Pelche element or a heater. The liquid feed control unit 5 has a structure in which the first layer 4 is deformed by using air, but the first layer 4 may be deformed by using water or oil. Further, the second layer 3 is preferably made of a material having a small coefficient of linear expansion, and is preferably formed of polyimide, polyester, Teflon (registered trademark) or the like.

As described above, according to the present embodiment, it is possible to suppress deterioration of contact thermal resistance due to thermal deformation and to provide a high-speed and highly accurate liquid feed cartridge for a temperature control device.

FIG. 7 is a cross-sectional view illustrating the structure of the temperature control device according to the second embodiment according to another embodiment of the present invention, and FIG. 8 is a diagram showing a modified example of FIG. 7. The present embodiment differs from the first embodiment in that the second layer 3 has the adhesive layer 14. Other configurations are the same as those in the first embodiment, and the same components as those in the first embodiment are designated by the same reference numerals, and the description overlapping with the first embodiment will be omitted.

As shown in FIG. 7, the adhesive layer 14 is formed on both sides of the second layer 3. Here, the adhesive layer 14 includes a flow path 8 formed in the liquid feeding tip 2, a first liquid feeding valve 9a formed in the liquid feeding control unit 5, a second liquid feeding valve 9b, and a third liquid feeding valve 9c. Selectively does not have the adhesive layer 14.
According to the above-described embodiment, the second layer 3 is adhered to the liquid feeding tip 2 and the first layer 4 via the adhesive layer 14, and the gap between the second layer 3 and the liquid feeding tip 2 and the first layer are adhered to each other. The leakage of liquid and the leakage of vapor from the gap between the layer 4 and the second layer 3 of the above layer 4 are suppressed.

Further, since the second layer 3 is adhered to the temperature control block 6, the contact state of the second layer 3 with the temperature control block 6 does not change, and the temperature control state becomes stable. However, when responsiveness is emphasized, the portion of the adhesive layer 14 in contact with the temperature control block 6 is removed (as shown in FIG. 8), and the second layer 3 and the temperature control block 6 are brought into direct contact with each other. You may.

As described above, according to the present embodiment, in addition to the effect of the first embodiment, the gap between the second layer 3 and the liquid feeding tip 2 and the gap between the first layer 4 and the second layer 3 are provided by the adhesive layer 14. Leakage of liquid and vapor from the air are suppressed. Further, since the second layer 3 is adhered to the temperature control block 6, the contact state of the second layer 3 with the temperature control block 6 does not change, and the temperature control state becomes stable.

FIG. 9 is a cross-sectional view illustrating the structure of the temperature control device according to the third embodiment according to another embodiment of the present invention. In this embodiment, the convex portion 15 is provided around the temperature control flow path 8a in the liquid feeding tip 2, which is different from the first embodiment. Other configurations are the same as those in the first embodiment, and the same components as those in the first embodiment are designated by the same reference numerals, and the description overlapping with the first embodiment will be omitted.

As shown in FIG. 9, the flow path 8 is formed by the concave portion formed in the liquid feeding tip 2, and the convex portion 15 is provided around the temperature control flow path 8a. As a result, the pressure for pressing the second layer 3 only around the temperature control flow path 8a increases, and it is possible to suppress the leakage of liquid and the leakage of steam from the temperature control flow path 8a. However, the convex portion 15 formed around the temperature control flow path 8a is not limited to one step, and for example, a concave portion is formed in the temperature control block 6 to form a comb tooth shape to further press the pressing surface. The pressure may be increased.

According to the above-mentioned embodiment, in addition to the effect of the first embodiment, it is possible to suppress the leakage of the liquid from the temperature control flow path 8a and the leakage of the vapor.

FIG. 10 is a cross-sectional view illustrating the structure of the temperature control device according to the fourth embodiment according to another embodiment of the present invention. In this embodiment, the liquid feeding tip 2 and the first layer 4 are brought into close contact with each other, and the second layer 3 is constructed on the lower surface of the first layer 4, which is different from the first embodiment. Other configurations are the same as those in the first embodiment, and the same components as those in the first embodiment are designated by the same reference numerals, and the description overlapping with the first embodiment will be omitted.

As shown in FIG. 10, the structure is such that the first layer 4 is brought into close contact with the liquid feeding cartridge 1 and the second layer 3 is brought into close contact with the lower surface thereof. That is, as compared with the above-mentioned Example 1, the flow path 8 is NC (normally closed) in this Example. Therefore, since the liquid feeding tip 2 and the first layer 4 made of an elastic body can be brought into close contact with each other, it is possible to suppress liquid leakage and steam leakage from the temperature control flow path 8a.

However, the second layer 3 may have an adhesive layer, and the contact thermal resistance at the interface is reduced by applying a heat conductive sheet or heat conductive grease to the interface between the temperature control block 6 and the temperature control flow path 8a. It may be a structure.

As described above, according to the present embodiment, in addition to the effect of the first embodiment, it is possible to suppress liquid leakage and steam leakage from the temperature control flow path 8a.

FIG. 11 is a cross-sectional view illustrating the structure of the temperature control device according to the fifth embodiment according to another embodiment of the present invention. In this embodiment, the first layer 4 is brought into close contact not only with the liquid feed control unit 5 but also with the temperature control block 6, and the second layer 3 is brought into close contact with the first layer 4 in the region of the temperature control block 6. It is different from the first embodiment in that the temperature control flow path 8a is constructed between the liquid feeding tip 2 and the liquid feeding tip 2. Other configurations are the same as those in the first embodiment, and the same components as those in the first embodiment are designated by the same reference numerals, and the description overlapping with the first embodiment will be omitted.

As shown in FIG. 11, the first layer 4 is brought into close contact with not only the liquid feed control unit 5 but also the temperature control block 6, and the second layer 3 is further attached to the first layer 4 in the region of the temperature control block 6. It has a structure that makes it adhere to. That is, as compared with the above-mentioned Example 1, in this Example, the first layer 4 is not provided with the opening 4a (FIG. 3) having substantially the same shape as the temperature control flow path 8a, and the first layer 4 is not provided. There is no opening. Therefore, it is possible to prevent liquid leakage and steam leakage from the temperature control flow path 8a to the outside.

In particular, when the second layer 3 is limited to the region of the temperature control block 6, the second layer 3 and the liquid feeding tip 2 are in close contact with each other around the temperature control flow path 8a, resulting in liquid leakage or steam. Can be prevented from leaking.

As described above, according to the present embodiment, in addition to the effect of the first embodiment, it is possible to prevent liquid leakage and steam leakage from the temperature control flow path 8a.

The present invention is not limited to the above-described embodiment, and includes various modifications. For example, the above-described embodiment has been described in detail in order to explain the present invention in an easy-to-understand manner, and is not necessarily limited to the one including all the described configurations. Further, it is possible to replace a part of the configuration of one embodiment with the configuration of another embodiment, and it is also possible to add the configuration of another embodiment to the configuration of one embodiment. Further, it is possible to add / delete / replace the configurations of other embodiments with respect to a part of the configurations of each embodiment.

1 ... Liquid feed cartridge, 2 ... Liquid feed chip, 3 ... Second layer, 4 ... First layer, 5 ... Liquid feed control unit, 6 ... Temperature control block, 7 ... Temperature control element, 8 ... Flow path, 8a ... temperature control flow path, 9a ... first liquid feed valve, 9b ... second liquid feed valve, 9c ... third liquid feed valve, 10 ... introduction hole, 11 ... discharge hole, 12 ... air flow path, 13 ... solution, 14 ... adhesive layer, 15 ... convex part, 16 ... temperature control part, 20 ... temperature control device

0003
Provided is a liquid feed cartridge for a moderate temperature control device.
Means for Solving Problems [0007]
In order to solve the above problems, the liquid feed cartridge for a temperature control device according to the present invention has a liquid feed that forms a flow path in a gap between a second layer that can be arranged in the temperature control unit and the second layer. Outside the region of the temperature control unit, the chip is arranged on the upper side of the liquid feeding chip, which is the side of the liquid feeding chip, or on the lower side of the second layer, and is made of an elastic body and described above. A first layer having a linear expansion coefficient larger than that of the second layer is provided, and a solution is introduced into the gap between the second layer and the liquid feeding chip in the region of the temperature control unit, and the solution is introduced. In a part outside the region of the temperature adjusting unit, the solution is introduced into the gap between the first layer and the liquid feeding chip.
[0008]
Further, the liquid feed cartridge for another temperature control device according to the present invention includes a liquid feed chip that forms a flow path in the gap between the second layer that can be arranged in the temperature control unit and the second layer. A first layer, which is arranged on the lower side opposite to the liquid feeding chip from the second layer, is made of an elastic body, and has a larger linear expansion coefficient than the second layer, is provided. In the region of the temperature control unit, the solution is introduced into the gap between the second layer and the liquid feed chip, and in a part outside the region of the temperature control unit, the first layer and the feed feed. It is characterized in that the solution is introduced into the gap with the liquid chip.
Effect of the invention [0009]
According to the present invention, it is possible to suppress deterioration of contact thermal resistance due to thermal deformation and to provide a high-speed and highly accurate liquid feed cartridge for a temperature control device.
Issues, configurations and effects other than those described above will be clarified by the following description of the embodiments.
Brief Description of Drawings [0010]
FIG. 1 is a schematic top view of a temperature control device according to a first embodiment of the present invention.
FIG. 2 is a schematic side view of the temperature control device according to the first embodiment.
FIG. 3 is a configuration diagram of a liquid feeding cartridge shown in FIGS. 1 and 2.
4 is a cross-sectional view taken along the line AA' explaining the structure of the temperature control device in FIG. 1. FIG.
5 is a cross-sectional view taken along the line BB'explaining the structure of the temperature control device in FIG. 1. FIG.
FIG. 6A is a diagram showing a solution introduction state of the liquid feed cartridge according to Example 1.
FIG. 6B is a diagram showing a closed flow path of the liquid feed cartridge according to the first embodiment.
[FIG. 6C] A state in which the solution of the liquid feed cartridge according to Example 1 is held in the temperature control flow path.

0003
Provided is a liquid feed cartridge for a moderate temperature control device.
Means for Solving Problems [0007]
In order to solve the above problems, the liquid feed cartridge for a temperature control device according to the present invention has a liquid feed that forms a flow path in a gap between a second layer that can be arranged in the temperature control unit and the second layer. The chip and the outside of the region of the temperature control unit are arranged on the upper side of the liquid feeding chip on the side of the second layer or on the lower side of the second layer, and are made of an elastic body and described above. A first layer having a linear expansion coefficient larger than that of the second layer is provided, and the liquid feeding cartridge is formed by the first layer, the second layer, and the liquid feeding chip, and the liquid feeding cartridge is fed. It is removable from the upper surface of the liquid control unit, and within the region of the temperature control unit, the solution is introduced into the gap between the second layer and the liquid feed chip, and is outside the region of the temperature control unit. Further, in the region having the liquid feed valve formed in the recess of the liquid feed control unit, the solution is introduced into the gap between the first layer and the liquid feed chip.
[0008]
Further, the other liquid feed cartridge for a temperature control device according to the present invention includes a liquid feed chip that forms a flow path in a gap between a second layer that can be arranged in the temperature control unit and the second layer. A first layer, which is arranged on the lower side opposite to the liquid feeding chip from the second layer, is made of an elastic body, and has a larger linear expansion coefficient than the second layer, is provided. A liquid feed cartridge is formed by the first layer, the second layer, and the liquid feed chip, and the liquid feed cartridge is removable from the upper surface of the liquid feed control unit and is within the region of the temperature control unit. Is a region where the solution is introduced into the gap between the second layer and the liquid feed chip, and is outside the region of the temperature control unit and in the region of the liquid feed valve formed in the recess of the liquid feed control unit. Is characterized in that the solution is introduced into the gap between the first layer and the liquid feeding chip.
Effect of the invention [0009]
According to the present invention, it is possible to suppress deterioration of contact thermal resistance due to thermal deformation and to provide a high-speed and highly accurate liquid feed cartridge for a temperature control device.
Issues, configurations and effects other than those described above will be clarified by the following description of the embodiments.
Brief Description of Drawings [0010]
FIG. 1 is a schematic top view of a temperature control device according to a first embodiment of the present invention.
FIG. 2 is a schematic side view of the temperature control device according to the first embodiment.
FIG. 3 is a configuration diagram of a liquid feeding cartridge shown in FIGS. 1 and 2.
4 is a cross-sectional view taken along the line AA' explaining the structure of the temperature control device in FIG. 1. FIG.
5 is a cross-sectional view taken along the line BB'explaining the structure of the temperature control device in FIG. 1. FIG.
FIG. 6A is a diagram showing a solution introduction state of the liquid feed cartridge according to Example 1.
FIG. 6B is a diagram showing a closed flow path of the liquid feed cartridge according to the first embodiment.
[FIG. 6C] A state in which the solution of the liquid feed cartridge according to Example 1 is held in the temperature control flow path.

Claims (7)

A second layer that can be placed in the temperature controller,
A liquid feeding tip that forms a flow path in the gap between the second layer and the liquid feeding tip.
Outside the region of the temperature control unit, the second layer is arranged on the upper side of the liquid feeding tip on the side of the liquid feeding tip or on the lower side of the second layer, is made of an elastic body, and has the second layer. A liquid feed cartridge for a temperature control device, comprising: a first layer having a coefficient of linear expansion larger than that of the layer. A second layer that can be placed in the temperature controller,
A liquid feeding tip that forms a flow path in the gap between the second layer and the liquid feeding tip.
It is provided with a first layer which is arranged on the lower side opposite to the liquid feeding tip from the second layer, is made of an elastic body, and has a larger linear expansion coefficient than the second layer. Liquid feed cartridge for temperature control device. In the liquid feed cartridge for a temperature control device according to claim 1 or 2.
For a temperature control device, the liquid feeding cartridge is formed by the first layer, the second layer, and the liquid feeding tip, and the liquid feeding cartridge is detachable from the upper surface of the liquid feeding control unit. Liquid feed cartridge. In the liquid feed cartridge for a temperature control device according to claim 3,
The liquid feed tip has different flow paths, that is, a flow path and a temperature control flow path.
The second layer has an opening in a region where the second layer and the flow path of the liquid feeding tip overlap.
A liquid feed cartridge for a temperature control device, characterized in that the liquid feed chip, the second layer, and the first layer are laminated in this order. In the liquid feed cartridge for a temperature control device according to claim 3,
The second layer is a liquid feed cartridge for a temperature control device, which has adhesive layers on both sides thereof. In the liquid feed cartridge for a temperature control device according to claim 3,
The second layer has adhesive layers on both sides thereof.
A liquid feed cartridge for a temperature control device, wherein the adhesive layer is removed in a region corresponding to the temperature control unit. In the liquid feed cartridge for a temperature control device according to claim 4.
A liquid feeding cartridge for a temperature control device, characterized in that a convex portion is provided around a temperature control flow path of the liquid feeding chip, and the convex portion presses against the second layer.

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