JPWO2020165425A5 - - Google Patents

Claims (13)

2. A complex comprising an inclusion body comprising a coupling peptide coupled to a partner peptide via a covalent isopeptide bond between the coupling peptide and the partner peptide , the coupling peptide constituting the linkage system. a conjugate that is a polymer of amino acids that binds one of the two peptides and the coupling peptide has the function of binding to a partner peptide. 2. The conjugate of claim 1, wherein said coupling peptide comprises one residue involved in said isopeptide bond and said partner peptide comprises the other residue involved in said isopeptide. The conjugate of any one of claims 1-2, wherein the coupling peptide and partner peptide are derived from Gram-positive or Gram-negative bacterial proteins. The conjugate of any one of claims 1-3, wherein the coupling peptide is selected from the group consisting of SpyTag, KTag, SnoopTag, SpyTag002, SpyTag003, SpyTag0128, SdyTag, DogTag, SnoopTagJr, and BDTag. The conjugate of any one of claims 1-4, wherein the partner peptide is selected from the group consisting of SpyTag, KTag, SpyCatcher, SnoopCatcher, SpyCatcher002, SpyCatcher003, SpyCatcher0128, SdyCatcher, DogTag, SnoopTagJr, and BDTag. . 前記カップリングペプチド及びパートナーペプチドが、ＳｐｙＴａｇ－ＳｐｙＣａｔｃｈｅｒ、ＳｐｙＴａｇ－ＳｐｙＣａｔｃｈｅｒ００２、ＳｎｏｏｐＴａｇ－ＳｎｏｏｐＣａｔｃｈｅｒ、ＳｐｙＴａｇ００２－ＳｐｙＣａｔｃｈｅｒ００２、ＳｐｙＴａｇ００２－ＳｐｙＣａｔｃｈｅｒ、ＳｐｙＴａｇ００３－ＳｐｙＣａｔｃｈｅｒ００３、ＳｐｙＴａｇ０１２８－ＳｐｙＣａｔｃｈｅｒ０１２８、ＳｄｙＴａｇ－ＳｄｙＣａｔｃｈｅｒ、ＫＴａｇ－ＳｐｙＴａｇ、ＳｐｙＴａｇ－ＫＴａｇ、ＤｏｇＴａｇ - a conjugate according to any one of claims 1 to 3, which forms a linked pair selected from the group consisting of SnoopTagJr, SnoopTagJr-DogTag, SpyTag-BDTag and BDTag-SpyTag. A conjugate according to any one of claims 1 to 6 , further comprising at least one protein of interest (PDI) or part thereof. 8. The conjugate of claim 7 , wherein said protein of interest or portion thereof is an antigen or fragment thereof. The complex of any one of claims 1-8 , wherein said inclusion body further comprises an inclusion body forming sequence. A conjugate according to any one of claims 1 to 9 , wherein said partner peptide comprises additional moieties such as targeting moieties . A composition comprising a conjugate according to any one of claims 1-10 . A conjugate according to any one of claims 1 to 10 or a composition according to claim 11 for use as a diagnostic, prognostic, prophylactic or therapeutic agent. 11. A method of producing a conjugate according to any one of claims 1-10 , wherein an inclusion body containing a coupling peptide is bound to a partner peptide via a covalent isopeptide bond between the coupling peptide and the partner peptide. to thereby form said complex.