JPWO2020041783A5 - - Google Patents

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JPWO2020041783A5
JPWO2020041783A5 JP2021509222A JP2021509222A JPWO2020041783A5 JP WO2020041783 A5 JPWO2020041783 A5 JP WO2020041783A5 JP 2021509222 A JP2021509222 A JP 2021509222A JP 2021509222 A JP2021509222 A JP 2021509222A JP WO2020041783 A5 JPWO2020041783 A5 JP WO2020041783A5
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Japan
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pmp
pmps
cells
modified
plant
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JP2021509222A
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JP2021533794A (en
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Priority claimed from PCT/US2019/048046 external-priority patent/WO2020041783A1/en
Publication of JP2021533794A publication Critical patent/JP2021533794A/en
Publication of JPWO2020041783A5 publication Critical patent/JPWO2020041783A5/ja
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植物メッセンジャーパック（ＰＭＰ）を標的細胞に送達する方法であって、外因性のイオン化可能な脂質を含むＰＭＰを前記標的細胞に導入することを含み、前記外因性のイオン化可能な脂質を含む前記ＰＭＰは、非修飾ＰＭＰと比べて、前記標的細胞による増加した取込みを有する、方法。 A method of delivering a plant messenger pack (PMP) to a target cell, comprising introducing a PMP comprising exogenous ionizable lipids into said target cell, said PMP comprising said exogenous ionizable lipid. has increased uptake by said target cell compared to unmodified PMP. 前記修飾ＰＭＰは、少なくとも１％、５％、１０％、２０％、３０％、４０％、５０％、６０％、７０％、８０％、９０％又は９０％超のイオン化可能な脂質を含む、請求項１に記載の方法。 said modified PMP comprises at least 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or more than 90% ionizable lipids; The method of claim 1. 前記修飾ＰＭＰは、少なくとも１％、５％、１０％、２０％、３０％、４０％、５０％、６０％、７０％、８０％、９０％又は９０％超の、植物細胞外小胞（ＥＶ）に由来する脂質を含む、請求項１に記載の方法。 Said modified PMP comprises at least 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% or more than 90% of plant extracellular vesicles ( 2. The method of claim 1, comprising lipids derived from EV). 前記増加した細胞取込みは、非修飾ＰＭＰと比べて、少なくとも１％、２％、５％、１０％、１５％、２０％、３０％、４０％、５０％、６０％、７０％、８０％、９０％又は１００％の増加した細胞取込みである、請求項１に記載の方法。 said increased cellular uptake is at least 1%, 2%, 5%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80% compared to unmodified PMP , 90% or 100% increased cellular uptake. 前記修飾ＰＭＰは、異種機能性薬剤を含む、請求項１に記載の方法。 2. The method of claim 1, wherein said modified PMP comprises a heterofunctional agent. 前記異種機能性薬剤は、前記複数のＰＭＰの各々によって封入される、請求項５に記載の方法。 6. The method of claim 5, wherein said heterofunctional agent is encapsulated by each of said plurality of PMPs. 前記異種機能性薬剤は、前記複数のＰＭＰの各々の表面に包埋される、請求項５に記載の方法。 6. The method of claim 5, wherein said heterofunctional agent is embedded on the surface of each of said plurality of PMPs. 前記異種機能性薬剤は、前記複数のＰＭＰの各々の表面に共役される、請求項５に記載の方法。 6. The method of claim 5, wherein said heterofunctional agent is conjugated to the surface of each of said plurality of PMPs. 前記細胞は、植物細胞である、請求項１に記載の方法。 2. The method of claim 1, wherein said cells are plant cells. 前記細胞は、細菌細胞である、請求項１に記載の方法。 2. The method of claim 1, wherein said cells are bacterial cells. 前記細胞は、真菌細胞である、請求項１に記載の方法。 2. The method of claim 1, wherein said cells are fungal cells. 非修飾ＰＭＰと比べて増加した細胞取込みを有する複数の修飾ＰＭＰを含む植物メッセンジャーパック（ＰＭＰ）組成物であって、
複数の修飾ＰＭＰは、外因性のイオン化可能な脂質を含む、組成物。 A plant messenger pack (PMP) composition comprising a plurality of modified PMPs having increased cellular uptake compared to unmodified PMPs ,
A composition, wherein the plurality of modified PMPs comprises an exogenous ionizable lipid .
JP2021509222A 2018-08-24 2019-08-24 Modified plant messenger pack and its use Pending JP2021533794A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862722576P 2018-08-24 2018-08-24
US62/722,576 2018-08-24
PCT/US2019/048046 WO2020041783A1 (en) 2018-08-24 2019-08-24 Modified plant messenger packs and uses thereof

Publications (2)

Publication Number Publication Date
JP2021533794A JP2021533794A (en) 2021-12-09
JPWO2020041783A5 true JPWO2020041783A5 (en) 2022-09-01

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JP2021509222A Pending JP2021533794A (en) 2018-08-24 2019-08-24 Modified plant messenger pack and its use

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US (2) US20210196632A1 (en)
EP (1) EP3840728A4 (en)
JP (1) JP2021533794A (en)
KR (1) KR20210049138A (en)
CN (1) CN112739327A (en)
AU (1) AU2019325698A1 (en)
BR (1) BR112021003311A2 (en)
CA (1) CA3109958A1 (en)
CL (2) CL2021000456A1 (en)
CO (1) CO2021003128A2 (en)
EA (1) EA202190368A1 (en)
IL (1) IL280935A (en)
MA (1) MA53443A (en)
MX (1) MX2021001980A (en)
PH (1) PH12021550354A1 (en)
SG (1) SG11202101778TA (en)
WO (1) WO2020041783A1 (en)

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AU2020340290A1 (en) * 2019-08-24 2022-04-07 Flagship Pioneering Innovations Vi, Llc Modification of plant messenger packs with charged lipids
WO2021237215A1 (en) * 2020-05-22 2021-11-25 University Of Louisville Research Foundation, Inc. Compositions and methods for preventing and/or treating microbial infections
CN111748562B (en) * 2020-06-15 2022-04-29 华南农业大学 Coding gene of Rhizoctonia solani Atg22 protein, target fragment Rsatg22 and application thereof
CN112646734B (en) * 2020-12-31 2022-11-29 广西壮族自治区林业科学研究院 Orchid mycorrhizal fungus PF06 and application thereof
IL309247A (en) * 2021-06-14 2024-02-01 Flagship Pioneering Innovations Vi Llc Modification of plant messenger packs
CN114544800B (en) * 2022-01-14 2023-07-11 南通市疾病预防控制中心 Method for detecting methoxy acrylic acid ester bactericide by molecular sieve series solid phase extraction
CN115161215A (en) * 2022-03-11 2022-10-11 中国农业大学 Fermentation method of bifidobacterium and application thereof
CN114958657B (en) * 2022-05-10 2023-09-05 广东省科学院生物与医学工程研究所 Application of Chitinophaga eiseniae in degradation of feather meal to production of biosurfactant
CN117363664A (en) * 2023-08-03 2024-01-09 长春师范大学 Method for extracting dibutyl phthalate from endophyte Bipolaris sp

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