JPWO2020037270A5 - - Google Patents

Claims (21)

A method of reducing nucleic acid contamination on a surface comprising:
contacting the nucleic acid-contaminated surface with a composition comprising modified pectin;
The modified pectin contains a plurality of amino groups,
Said modified pectin has the formula

an amidated pectin comprising one or more monomeric units represented by its tautomers, isomers or salts;
where n is 0, 1, 2, or 3;
R ¹ is H or C ₁ -C ₃ alkyl,
X at each occurrence is independently C2 - _{C4 alkylene} or _{C4 -} C6 heteroalkylene;
Y is C2 _- C3 alkylene or _{C4 -} C6 _{heteroalkylene} , and
R2 and R3 ^are independently H or C1 ^- _{C3 alkyl} ;
Method. 2. The method of claim 1 , wherein said amidated pectin has a molecular weight between about 0.5 kDa and about 500 kDa. 2. The method of claim 1 , wherein the amidated pectin has a molecular weight of about 100 kDa to about 300 kDa. 2. The method of claim 1 , wherein the composition comprising said amidated pectin is a solution of said amidated pectin. 5. The method of claim 4 , wherein said amidated pectin is present in solution at a concentration of about 0.01% to about 5%. 5. The method of claim 4 , wherein said amidated pectin is present in solution at a concentration of about 0.1% to about 0.5%. 5. The method of claim 4 , wherein said amidated pectin is present in solution at a concentration of about 1 ug/mL to about 1000 ug/mL. 2. The method of claim 1 , wherein said amidated pectin is amidated citrus pectin or amidated apple pectin. 2. The method of Claim 1 , wherein the composition is present on a swab, wipe, cloth, filter, or sponge. 2. The method of claim 1 , wherein the surface is an instrument surface or a laboratory bench surface. A method of reducing nucleic acid contamination in a solution, comprising:
contacting the nucleic acid-contaminated solution with a solid support comprising modified pectin covalently attached to the solid support;
The modified pectin contains a plurality of amino groups,
Said modified pectin has the formula

an amidated pectin comprising one or more monomeric units represented by its tautomers, isomers or salts;
where n is 0, 1, 2, or 3;
R ¹ is H or C ₁ -C ₃ alkyl,
X, at each occurrence, is independently C2 - _{C4 alkylene} or _{C4 -} C6 heteroalkylene;
Y is C2 _- C3 alkylene or _{C4 -} C6 _{heteroalkylene} , and
R2 and R3 ^are independently H or C1 ^- _{C3 alkyl} ;
Method. 12. The method of claim 11 , wherein said solid support is magnetic beads, glass beads, polystyrene beads, polystyrene filters, or glass filters. 12. The method of Claim 11 , wherein said solid support is a swab, wipe, cloth, filter, or sponge. A method of reducing aerosolized nucleic acid contamination in the air comprising:
contacting air contaminated with aerosolized nucleic acid with a composition comprising modified pectin;
The modified pectin contains a plurality of amino groups,
Said modified pectin has the formula

an amidated pectin comprising one or more monomeric units represented by its tautomers, isomers or salts;
where n is 0, 1, 2, or 3;
R ¹ is H or C ₁ -C ₃ alkyl,
X, at each occurrence, is independently C2 - _{C4 alkylene} or _{C4 -} C6 heteroalkylene;
Y is C2 _- C3 alkylene or _{C4 -} C6 _{heteroalkylene} , and
R2 and R3 ^are independently H or C1 ^- _{C3 alkyl} ;
Method. 15. The method of claim 14 , wherein contacting air contaminated with aerosolized nucleic acid comprises passing air through a solution comprising amidated pectin. 15. The method of claim 14 , wherein contacting air contaminated with aerosolized nucleic acid comprises passing the air through a filter containing amidated pectin. 17. The method of claim 16 , wherein said amidated pectin is covalently attached to said filter. 15. The method of claim 14, wherein contacting air contaminated with aerosolized nucleic acid comprises passing air over a surface comprising amidated pectin covalently bound to the surface. 19. The method of any one of claims 1-18 , wherein said nucleic acid is the product of a nucleic acid amplification reaction. 20. The method of claim 19 , wherein said amplification reaction is the polymerase chain reaction. wherein the modified pectin has the formula

or an amidated pectin comprising one or more monomeric units represented by their isomers, salts or tautomers.