JPWO2020032215A1 - Deodorant composition - Google Patents

Abstract

The present invention relates to a deodorant composition that suppresses body odor derived from the body by oral ingestion, and relates to a deodorant composition containing polyphenols and polyphenol oxidase.

Description

The present invention relates to a deodorant composition effective in suppressing body odor.

In the modern super-aging society, improvement of quality of life (QOL) is required. In other words, the importance is placed on how to improve the quality of life and live better. For that purpose, it is an important factor to enhance and maintain good relationships and fellowship with the surroundings. Body odor is one of the major factors affecting the maintenance of this good interpersonal relationship. Since odors can make people uncomfortable, it is important to prevent and take measures against body odors in order to lead a comfortable life.

Body odor is roughly classified into two types, one derived from skin surface reaction and the other derived from the body. There have been many reports of deodorant compositions for the purpose of suppressing body odor, but most of them are targeted for deodorization of body odor generated by a reaction on the skin surface. Examples of body odors derived from skin surface reactions include axillary odors, foot odors, and aging odors, which are generated by decomposition of secretions such as sebum and sweat that do not have odors themselves by indigenous skin bacteria. It is an odor. Therefore, as a method for suppressing body odor derived from such a skin surface reaction, a deodorant agent such as a roll stick or a spray type by directly applying or spraying a deodorant on the skin surface is generally used.

On the other hand, body odor derived from the body is an odor generated in the body and released through the skin. More specifically, a chemical substance taken into the body is absorbed by the blood, carried by the bloodstream, and released through the skin. It is a blood-derived odor. Therefore, body odor derived from the body is halitosis emitted from the oral cavity caused by sulfur-containing compounds generated by decomposition of food residues in the oral cavity by bacteria, and low-molecular-weight malodorous components produced by decomposition of food in the stomach and intestines. It is possible to distinguish it from the excrement odor such as fecal odor and urine odor, which is the cause of the odor of the excrement itself. Due to the route of generation of body odor derived from the body, it is important to take care from the inside of the body instead of the surface of the skin. Examples of body odors emitted from the body through the skin include body odors generated by ingesting foods having a strong flavor such as garlic, and body odors generated by fatigue.

If the odor causes discomfort to a person, measures are taken to suppress the odor.
For example, Patent Document 1 describes a deodorant composition in which the deodorizing efficiency is remarkably improved by combining polyphenol and polyphenol oxidase. Patent Document 2 describes a deodorant composition containing a plant extract and an enzyme that oxidizes a phenolic compound. Patent Document 3 describes a method of suppressing the odor of animal excrement by ingesting a edible food containing a plant extract and an enzyme that oxidizes a phenolic compound.

Patent Documents 4 to 6 describe deodorant compositions in which the target of deodorization is garlic-derived body odor as an example of food-derived body odor.

Patent Document 7 reports on the suppression of the odor of aging by ingesting naturally-derived polyphenols orally.

Japanese Patent No. 3562668 Japanese Patent No. 3625976 Japanese Patent Application Laid-Open No. 2000-50814 Japanese Patent No. 4641073 Japanese Patent No. 4865905 Japanese Patent No. 5828658 Japanese Patent Application Laid-Open No. 2007-314472

However, although the deodorant compositions described in Patent Documents 1 to 3 describe the deodorizing effect for halitosis, environmental odor, or excrement odor, they are intended for body odor derived from the body. No tests have been conducted.
Further, in the deodorant compositions described in Patent Documents 4 to 6, only the deodorizing effect on bad breath when garlic is ingested has been tested, and by analogy with the deodorizing effect, it becomes a body odor. It just claims the effect on it. Therefore, these reports do not describe test examples that actually measured body odor.
The aging odor targeted in Patent Document 7 is generally an odor derived from a skin surface reaction, and no test has been conducted on the body odor derived from the body.

As described above, in the conventional technique, there has been no report example of a deodorant composition showing an effect on the odor generated in the body and emitted through the skin. Furthermore, in the prior art, there are reports that the deodorant effect on body odor is claimed by analogy with the effect on halitosis, even though the data obtained by actually measuring the body odor derived from the body is not shown. Therefore, there are few reports that clearly show the inhibitory effect of the deodorant composition by measuring the body odor derived from the body.
Therefore, an object of the present invention is to provide a deodorant composition capable of suppressing body odor derived from the body.

As a result of diligent research, the inventors have found that the coexistence of polyphenols and polyphenol oxidase can suppress body odor derived from the body. Therefore, the present invention relates to the following.
[1] A deodorant composition that suppresses body odor derived from the body by oral ingestion, and contains a polyphenol and a polyphenol oxidase.
[2] The deodorant composition according to [1], wherein the polyphenol contains at least one of an o-diphenol structure and a p-diphenol structure.
[3] The deodorant composition according to [1] or [2], wherein the polyphenol oxidase is an enzyme obtained from a plant extract.
[4] The deodorant composition according to any one of [1] to [3], wherein the content of polyphenol is 2 mmol or less in terms of catechol.
[5] A method for suppressing body odor derived from the body by orally ingesting the deodorant composition according to any one of [1] to [4].

According to the present invention, body odor derived from the body can be effectively suppressed only by ingesting the deodorant composition of the present invention orally.

FIG. 1 is a graph showing the measurement results of diallyl disulfide of Example 1 and Comparative Example 1. FIG. 2 is a graph showing the measurement results of allyl methyl sulfide of Example 1 and Comparative Example 1. FIG. 3 is a graph showing the measurement results of diallyl disulfide of Example 2 and Comparative Example 1. FIG. 4 is a graph showing the measurement results of allyl methyl sulfide of Example 2 and Comparative Example 1. FIG. 5 is a graph showing the measurement results of diallyl disulfide of Example 3 and Comparative Example 1. FIG. 6 is a graph showing the measurement results of allyl methyl sulfide of Example 3 and Comparative Example 1. FIG. 7 is a graph showing the measurement results of diallyl disulfide of Example 4 and Comparative Example 1. FIG. 8 is a graph showing the measurement results of allyl methyl sulfide of Example 4 and Comparative Example 1. FIG. 9 is a graph showing the measurement results of diallyl disulfide of Example 5 and Comparative Example 1. FIG. 10 is a graph showing the measurement results of allyl methyl sulfide of Example 5 and Comparative Example 1. FIG. 11 is a graph showing the measurement results of diallyl disulfides of Comparative Examples 1 to 3. FIG. 12 is a graph showing the measurement results of allyl methyl sulfide of Comparative Examples 1 to 3. FIG. 13 is a graph showing the measurement results of ammonia in Example 6 and Comparative Example 4. FIG. 14 is a graph showing the measurement results of ammonia in Example 7 and Comparative Example 4.

[Deodorant composition]
The present invention relates to a deodorant composition that suppresses body odor derived from the body by oral ingestion, and relates to a deodorant composition containing polyphenols and polyphenol oxidase.
In the present invention, by orally ingesting a deodorant composition containing polyphenols and polyphenol oxidase, the polyphenols are oxidized by the polyphenol oxidase in vivo to form a highly reactive quinone structure, and the quinone structure compound has a further malodor. As a result of reacting with the substance, it is presumed that the malodorous substance in the body is reduced and the deodorizing effect is obtained.

〔Polyphenol〕
The polyphenol, which is one component of the deodorant composition of the present invention, refers to all compounds having a polyphenol structure in which at least two hydroxy groups are bonded to the same benzene ring. Polyphenols also include glycosides.

Specific examples of polyphenols include, for example, apigenin, apigenin glycoside, acasetin, isolamnetin, isolamnetin glycoside, isoquercitrin, epicatechin, epicatechin gallate, epigallocatekin, epigallocatekingalate, esculetin, ethylprotocate. Cuate, ellagic acid, catechol, gamma acid, catechin, gardenin, galocatechin, caffeic acid, caffeic acid ester, chlorogenic acid, kenferol, kenferol glycoside, quercetin, quercetin glycoside, quercetagenin, genicetin, genicetin Sugars, gosipetin, gosipetin glycosides, gosipole, 4-dihydroxyanthraquinone, 1,4-dihydroxynaphthalene, cyanidin, cyanidin glycosides, sinensetin, diosmethin, diosmethine glycosides, 3,4'-diphenyldiol, cinnapic acid , Stearyl-β- (3,5-di-t-butyl-4-hydroxyphenyl) propionate, spinacetin, tangeretin, taxiphorin, tannic acid, daphnetin, tyrosine, delphinidin, delphinidin glycoside, teaflavin, teaflavin monogalate, teaflabin Bisgarate, trisetinidin, L-dopa, dopamine, naringenin, narindine, nordihydroguayaletic acid, noradrenaline, hydroquinone, vanillin, patchuletin, herbasetin, vanillyl alcohol, vanitrop, vanillin propylene glycol acetal, vanillic acid, bis (4- Hydroxyphenyl) sulfonic acid, bisphenol A, pyrocatechol, bitexin, 4,4'-biphenyldiol, 4-t-butylcatechol, 2-t-butylhydroquinone, protocatechuic acid, fluoroglusinol, phenolic resin, procyanidin, prodelphinidin , Floretin, Floretin glycosides, Fizetin, Forin, Ferbasetin, Fraxetin, Florizine, Peonydin, Peonidin glycosides, Perorgonidin, Peraggonidine glycosides, Petunidin, Petunidin glycosides, Hesperetin, Hesperezin Methyl asbestosate, ethyl asbestosate, dodecyl asbestosate, lauryl asbestosate, propyl asbestosate, butyl asbestosate, octyl asbestosate, etc.), mandiferin, malvidin, malvidin glycosides, mylicetin, mylicetin glycosides, 2,2 '-Methylenebis (4-methyl-6-t-butylpheno) , 2,2'-Methylenebis (4-ethyl-6-t-butylphenol), 2,2'-Methylenebis (4-methyl-6-t-butylphenol), 2,2'-Methylenebis (4-ethyl) -6-t-butylphenol), methyl atlasate, 4-methylcatechol, 5-methylcatechol, 4-methoxycatechol, 5-methoxycatechol, methylcatechol-4-carboxylic acid, 2-methylresorcinol, 5-methylresorcinol, Morin, limocitrin, limocitrin glycoside, limocitrol, luteolin, luteolin glycoside, luteolinidin, luteolinidin glycoside, rutin, resorcin, resorcinol, resorcinol, leucocyanidin, leukodelphinidin and the like can be mentioned.

Among these polyphenols, those having an o-diphenol structure and / or a p-diphenol structure are particularly preferable because they can contact with polyphenol oxidase and rapidly form a quinone structure. Specifically, flavonoids such as quercetin, epicatechol, and epigalocatechol and their glycosylates, gallic acid, gallic acid ester, chlorogenic acid, caffeic acid, caffeic acid ester, tannic acid, pyrocatechol, norl. Polyphenol compounds having an o-diphenol structure such as dihydroguaric acid, L-dopa, 4-methylcatechol, 5-methylcatechol, 4-methoxycatechol, 5-methoxycatechol, and hydroquinone are particularly preferable.

Polyphenols can be prepared by known methods, but may be purchased commercially or may be prepared synthetically. Furthermore, a polyphenol-containing fraction prepared from a plant can also be used. Also, plant extracts containing polyphenols can be used. As this plant extract, one prepared by a known method may be used, or a commercially available one may be used.

Examples of plants that obtain plant extracts include aloe, anis seed, elder, eleutherocock, obaco, orange flower, all spice, oregano, kanokosou, camo mill, capsicum pepper, cardamon, cassia, garlic, caraway seed. , Clove, Cumin Seed, Cola, Coriander Seed, Gobiko, Saffron, Sansho, Juniper Berry, Cinnamon, Ginger, Star Anis, St. Jones Walt, Cellory Seed, Sesame (Goma), Daiou, Taragon, Turmeric, Chisle, Dale Seed, Natsumeg, Nettle, Hibiscus, Hamamelis, Birch, Basil, Bitter Orange, Fennell, Primrose, Fenuglique, Belbena, Bay Laurel, Hop, Bordeaux, Horse Radish, Poppy Seed, Glutton, Marigold, Mallow, Majorum , Mustard, Milfoil, Mint Rivers, Melissa, Mace, Linden, Lindow, Rosehip, Rosemary, Mannenrou, Sunflower Seed, Grape Peel, Apple, Carrot Leaf, Banana, Strawberry, Anzu, Peach, Plum, Pineapple, Pear, Kaki , Sakurambo, papaya, mango, avocado, melon, biwa, fig, kiwi, prun, blueberry, blackberry, rasberry, vine kokemomo, coffee beans, cacao beans, grape seeds, grapefruit seeds, pecan nuts, cashew nuts, chestnuts, coconuts, peanuts, Walnut, green tea leaf, tea leaf, oolong tea leaf, bittersweet tea, mate tea, louis boss tea, tobacco, perilla leaf, niwa thyme, sage, lavender, spare mint, peppermint, santorisou, hisop, mebouki, marigold, dandelion, arch choke, German Kamirure, Kinmizuhiki, Kanzo, Anis, Nokogirisou, Eucalyptus, Wormwood, Perfume oil, Shishiudo, Koroha, Shishitogarashi, Uikyo, Togarashi, Coendro seed, Himeuikyo seed, Uikyo seed, Ginger, Western wasabi, Mayorana , Parsley, pepper, savory, taragon, turmeric, wasabi, inondo seeds, citrus fruits, pears, thyme, etc., and all vegetables such as carrots, gobos, peppers, cubs, potatoes. In particular, rosemary, sunflower seeds, grape peel, apples, carrot leaves, coffee (raw) beans, cacao (raw) beans, grape seeds, green tea leaves, black tea leaves, oolong tea leaves, pericarp, chicken thyme, sage, spearmint, peppermint. , Western pear, banana, thyme, quintuplet, mint are preferred. The plant can prepare a plant extract from a single plant or a plurality of plants. It is also possible to obtain a single plant extract and then mix it with a single plant extract of a different plant. The method for producing the plant extract is not particularly limited. The plant can also be used as a residue after being used for other purposes.

The extraction method may be any known method that can achieve the purpose. When the deodorant composition is for food and the polyphenol is obtained by, for example, solvent extraction, the solvent is also selected to be acceptable for the food. Examples of such a solvent include water, ethanol, propanol, butanol, acetone, hexane, propylene glycol, hydrous ethanol, hydrous propylene glycol and the like, but hot water, hydrous ethanol and hydrous propylene glycol are more preferable. The extraction method is not limited to solvent extraction, and there is no problem with supercritical extraction or the like.

[Polyphenol oxidase]
The polyphenol oxidase (phenolic compound oxidase), which is the other component of the deodorant composition of the present invention, is an enzyme having an action of oxidizing the above polyphenol to a compound having a quinone structure, or a phenol together with the action. It is an enzyme that has the effect of adding a phenolic hydroxyl group and oxidizing it to quinone.

The polyphenol oxidase may be any enzyme having such an action, and examples thereof include polyphenol oxidase, monophenol oxidase, oxidase producing hydrogen peroxide, and peroxidase. More specifically, laccase, tyrosinase, glucose oxidase, and peroxidase can be preferably mentioned.

In addition, polyphenol oxidase obtained from a plant extract or a fungal extract can also be used. As the enzyme-containing plant, fruits and vegetables such as apples, pears, and burdock are preferable. Examples of fungi containing enzymes include mushrooms of the genus Agaricus and Boletus genus such as mushrooms and bay bolete. Enzymes obtained from plant extracts are preferred from the standpoint of ease of handling, availability and flavor when taken orally.

As these enzymes, commercially available ones can be used, but they can also be prepared by using a known method. In addition, two or more types of enzymes can coexist.

The enzyme or enzyme-containing substance can be obtained by using a plant or fungus containing the enzyme as a raw material and treating it by a conventional method. For example, an extract from a plant containing the enzyme, an extract from a fungus containing the enzyme, or a freeze-dried powder containing the extract, for example, acetone powder can be exemplified. In the present invention, as long as it has the above-mentioned action, the content of the enzyme or the composition containing the enzyme is also within the range of the phenol oxidase of the present invention.

The content of polyphenol in the deodorant composition of the present invention is preferably 2.00 mmol or less, more preferably 1.69 mmol or less, and particularly preferably 0.85 mmol or less in terms of catechol. As long as the content of polyphenol is within such a range, it shows an inhibitory effect on body odor derived from the body. On the other hand, if the amount of polyphenol is too large, it is difficult to show an inhibitory effect on body odor derived from the body. The lower limit of the polyphenol content is preferably 0.20 mmol or more, more preferably 0.42 mmol or more in terms of catechol, from the viewpoint of deodorizing effect.

The content of the polyphenol oxidase in the deodorant composition of the present invention is preferably 12.5 Units or more, and more preferably 15 Units or more from the viewpoint of deodorizing power.

[Other ingredients]
The deodorant composition of the present invention may contain other components in addition to the above-mentioned polyphenols and polyphenol oxidases as long as the effects of the present invention are not impaired. Examples thereof include commonly used compounding agents such as carriers, stabilizers, bulking agents, pigments, antioxidants and fragrances.

The form of the deodorant composition of the present invention is, for example, a liquid state such as a liquid agent, a suspension agent or an emulsion, a semi-solid state such as a cream, a powder form, a granule, a capsule, a tablet, a sheet or the like. It can take any form such as, and is not particularly limited.

The polyphenol and the polyphenol oxidase may be ingested uniformly, or the polyphenol and the polyphenol oxidase may be ingested in separate forms at the same time.

[Body odor control method]
By orally ingesting the deodorant composition of the present invention, body odor derived from the body can be suppressed.
The odor targeted by the body odor control method of the present invention is a body odor derived from the body that is generated in the body and emitted from the skin. It is a blood-derived odor that is released through the skin. Examples of those classified into this include body odor derived from food and body odor derived from fatigue.
As a typical example of body odor generated in the body due to food origin, body odor emitted by ingestion of garlic is known. Garlic (Allium sativum) is a plant of the genus Allium in the family Amaryllidaceae. It is known that foods belonging to the genus Allium such as garlic and garlic smell because the components that are considered to be odorous substances are volatile sulfur compounds that emit a strong odor, such as diallyl disulfide and allyl methyl sulfide. There is. These substances produced by ingestion of garlic are the main causes of bad breath and body odor through the oral mucosa and various organs. When garlic is ingested, it suffers from bad breath due to its unique and intense aroma after eating. In addition, it is said that the garlic odor is caused not only by bad breath but also by body odor because the garlic-derived aroma component is metabolized in the body, and it is said to have high sustainability.
Ammonia is known as a body odor derived from fatigue. Ammonia is present in the blood as a metabolic product of proteins and amino acids, and is also released from the skin. In addition, it is considered that the produced ammonia is transferred from the muscle tissue to the blood due to the physical load such as exercise and fatigue such as mental stress. As described above, it has been clarified that the causative component of body odor due to fatigue is ammonia, and the deodorant composition of the present invention, which is also effective against nitrogen-containing compounds, can be obtained by ingesting high-protein foods. It seems that the body odor generated and the body odor derived from fatigue can also be suppressed.
Therefore, according to the body odor suppressing method of the present invention, body odor containing volatile sulfur compounds such as diallyl disulfide and allyl methyl sulfide and volatile nitrogen-containing compounds such as ammonia can be effectively suppressed.

The body odor emitted from the skin can be accurately collected and analyzed by using, for example, a skin gas collecting device (Japanese Patent No. 4654045).

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but the present invention is not limited thereto.

1. 1. Preparation of each sample [Reference Example 1] (Preparation of polyphenol-containing plant extract)
(1) Extract of raw coffee beans Raw coffee beans were crushed with a crusher (mesh 5 mm), water was added, and the beans were extracted at 85 to 95 ° C. for 2 hours. After filtering the extract, the filtrate was adsorbed on an XAD-2 (manufactured by Organo Corporation) column. After washing with water, the product eluted with methanol was concentrated to dryness to obtain a green coffee bean extract (chlorogenic acid content: 30%).
The chlorogenic acid content was measured by the HPLC method from the calibration curve based on the peak area ratio with the standard.
Column: GC (manufactured by Nacalai Tesque)
Developing solvent: acetonitrile, 0.01M phosphoric acid solution Detection: UV325nm

Similarly, each of the other polyphenol compounds was measured from the calibration curve based on the peak area ratio with each standard. The optimum detection wavelength was selected each time.
(2) Rosemary Extract 1 L of ethanol having a water content of 40 to 60% was added to 100 g of rosemary leaves and flowers, heated under reflux for 3 hours, and filtered at warm temperature to obtain a filtrate containing an antioxidant component. The operation of similarly extracting the residue with the same solvent was repeated twice more, and the obtained filtrates were combined. 500 ml of water was added to this extract to precipitate a water-insoluble antioxidant component, and 10 g of activated carbon was further added and stirred. The solution was left in a cold place overnight and then filtered to obtain a filtrate. This filtrate was concentrated under reduced pressure to obtain a water-soluble antioxidant component category (solid).
(3) Tea Extract 1 kg of sencha was extracted with 10 L of hot water at 90 ° C. for 1 hour while stirring, and the tea leaves were removed by filtration to obtain 8.3 L of an extract. This liquid was concentrated to 1 L, 1 L of acetone was added thereto, and the mixture was stirred, and the resulting insoluble matter was removed by centrifugation. 1 L of ethyl acetate was added to the supernatant, the mixture was stirred, and the mixture was allowed to stand for 30 minutes. The obtained ethyl acetate layer was concentrated under reduced pressure, converted to an aqueous layer, and then freeze-dried to obtain 97 g of tea phenol having a purity of 60%. This was used as a tea extract.
(4) Peppermint extract The dried peppermint leaves were crushed with a grinder and extracted with hot water at 85 to 95 ° C. for 2 hours. The extract was filtered and the filtrate was washed 3 times with hexane. The aqueous layer was dried to give a peppermint extract.
(5) Grape peel extract Ethanol was added to the grape peel (variety: Campbell variety), and then the mixture was stirred and extracted at 70 ° C. for 2 hours. The extract was concentrated to dryness and used as a grape peel extract.

[Reference Example 2] (Preparation of plant extract containing polyphenol oxidase)
(1) Burdock extract 400 L of acetone at −20 ° C. was added to 100 g of washed burdock, ground with a mixer, and then suction filtered. The residue was thoroughly washed with 500 ml of an 80% acetone-containing aqueous solution at 5 ° C., combined with the filtrate, acetone was distilled off, and then freeze-dried to obtain a powder. The yield was 20%. The enzyme specific activity was 50 Units / g.
(2) Prune extract The prune fruits were washed, seeded, crushed, washed with water, and freeze-dried to be powdered. The powder yield from the fruit was 3%. The enzyme specific activity was 30 Units / g.
(3) Pear extract Using 100 g of pear, a pear extract was obtained by the same operation as the preparation of the above-mentioned burdock extract. The yield was 15%. The enzyme specific activity was 63 Units / g.
(4) Apple extract Using 100 g of apples, a pear extract was obtained by the same operation as the preparation of the above-mentioned burdock extract. The yield was 18%. The enzyme specific activity was 53 Units / g.

(Measurement of specific activity)
Each of the above extracts was reacted with chlorogenic acid (Tokyo Kasei) 1.7 mg / L as a substrate in 5 ml phosphate buffer (pH 6.5) at 25 ° C. for 5 minutes to increase the absorbance at 420 nm of ultraviolet absorption by 1. The enzyme to be caused was defined as 1 unit (Units).

[Reference Example 3] (Mixture of enzyme and plant extract)
(1) The raw coffee bean extract obtained in Reference Example 1 (1) and the burdock extract obtained in Reference Example 2 (1) were mixed in equal amounts, and a 60-mesh pass was used as sample P1.
(2) The raw coffee bean extract obtained in Reference Example 1 (1) and the prune extract obtained in Reference Example 2 (2) were mixed in equal amounts, and a 60-mesh pass was used as sample P2.

[Reference Example 4] (Deodorant activity of the mixture)
After collecting 1.5 ml of an aqueous solution containing 1% by mass of the sample P1 obtained in Reference Example 3 (1) in a vial, each malodorous component in the headspace was added so as to have the following concentration [methyl mercaptan (CH). ₃ SH): 200 ppm, ammonia (NH ₃ ): 100 ppm, trimethylamine (TMA): 100 ppm, isovaleric acid: 10 ppm, isobutyric acid: 10 ppm]. After shaking at 25 ° C. for 10 minutes, the concentration of each malodorous component in the headspace was measured with a gas detector tube (manufactured by Gastec).
Table 1 shows the deodorizing effect for each malodorous component.
The deodorant rate was calculated according to the following formula.
Deodorant rate (%) = {(measured value of blank)-(measured value of sample)} / (measured value of blank) x 100

Sample P1 showed an excellent deodorizing effect on methyl mercaptan, ammonia and trimethylamine. It also showed a deodorizing effect on lower fatty acids such as isovaleric acid and isobutyric acid.

2. Body odor suppression test procedure (method)
Each sample (raw coffee bean extract, burdock extract or prune extract, a mixture of both) was ingested by the subjects, and the body odor suppressing effect was evaluated.
Specifically, first, three adult male subjects were allowed to ingest 50 g of heated garlic from 9:00 am. Five minutes after ingesting the garlic, each sample was dissolved in 200 ml of water and ingested. Then, skin gas was collected over time before and after ingestion of garlic. The test was conducted on the same day for all three subjects. Since the test results showed the same tendency in all three subjects, the results of one of the subjects are described as an example.
The skin gas is a skin gas collecting device using a silica monolith-based adsorbent (MonoTrap (registered trademark) DCC18 manufactured by GL Sciences Co., Ltd.) as a collecting agent (Skin gas collecting device described in Japanese Patent No. 4654045). It was collected from a predetermined position on the left forearm by (a type of). First, as skin gas before ingesting garlic, one hour's worth from 8:00 am to 9:00 am was collected. For skin gas after ingestion of garlic, measurement starts at 9:30 am, every 30 minutes from 9:30 am to 10:00 am and from 10 am to 10:30 am, and 1 hour thereafter. Collection was carried out at intervals.
After collection, transfer the adsorbent to a vial for thermal desorption and use a gas chromatography / mass spectrometer with a thermal desorption device (GC device: 6890N manufactured by Azilent Technology Co., Ltd., MS device: Q1000GCMkII manufactured by JEOL Ltd.). The amounts of diallyl disulfide and allyl methyl sulfide were measured, and the emission flux (ngcm ^{- 2h-1} ) was determined.
The emission of skin gas released from the skin is defined as "emission flux" in Japanese Patent No. 4654045, and is calculated by the following formula.
J = W / (St)
J: Dissipative flux of skin gas W: Amount of skin gas collected by the collecting material S: Opening area t: Collection time

[Examples 1, 3 to 5]
Five minutes after completely ingesting 50 g of heated garlic, the amount of sample P1 shown in Table 2 (1: 1 mixture of raw coffee bean extract and burdock extract prepared in Reference Example 3 (1)) was added to water. It was dissolved in 200 ml and ingested.

[Example 2]
Five minutes after ingesting the heated garlic, 1 g of sample P2 (1: 1 mixture of raw coffee bean extract and prune extract prepared in Reference Example 3 (2)) was dissolved in 200 ml of water and ingested. ..

[Comparative Example 1]
Five minutes after ingesting the heated garlic, 200 ml of water was ingested.

[Comparative Example 2]
Five minutes after the ingestion of the heated garlic was completed, 0.5 g of the green coffee bean extract prepared in Reference Example 1 (1) was dissolved in 200 ml of water and ingested.

[Comparative Example 3]
Five minutes after ingesting the heated garlic, 0.5 g of the burdock extract prepared in Reference Example 2 (1) was dissolved in 200 ml of water and ingested.

Table 2 below shows the intake of the sample (P1 or P2), the number of moles of the polyphenolic substance (catechol equivalent (mmol)), and the number of units of the polyphenol oxidase in each Example and Comparative Example.
The catechol-equivalent value (mmol) of polyphenol was calculated from the molecular weight and intake of the target polyphenol and the molecular weight of catechol.
Table 2 shows the results of the body odor suppression test of each Example and Comparative Example.

The measurement results of diallyl disulfides of Example 1 and Comparative Example 1 are shown in FIG.
The measurement results of allyl methyl sulfide of Example 1 and Comparative Example 1 are shown in FIG.
The measurement results of diallyl disulfide of Example 2 and Comparative Example 1 are shown in FIG.
The measurement results of allyl methyl sulfide of Example 2 and Comparative Example 1 are shown in FIG.
The measurement results of diallyl disulfide of Example 3 and Comparative Example 1 are shown in FIG.
The measurement results of allyl methyl sulfide of Example 3 and Comparative Example 1 are shown in FIG.
The measurement results of diallyl disulfides of Example 4 and Comparative Example 1 are shown in FIG.
The measurement results of allyl methyl sulfide of Example 4 and Comparative Example 1 are shown in FIG.
The measurement results of diallyl disulfide of Example 5 and Comparative Example 1 are shown in FIG.
The measurement results of allyl methyl sulfide of Example 5 and Comparative Example 1 are shown in FIG.
The measurement results of diallyl disulfides of Comparative Examples 1 to 3 are shown in FIG.
The measurement results of allyl methyl sulfide of Comparative Examples 1 to 3 are shown in FIG.

Evaluation Criteria for Body Odor Suppression Effect in Table 2 ⊚: Very effective in suppressing body odor originating in the body.
◯: Effective in suppressing body odor derived from the body.
X: Not effective in suppressing body odor derived from the body.

From each measurement result, it can be seen that the emission amount of diallyl disulfide and allyl methyl sulfide is reduced by ingestion of a mixture of polyphenolic substance (raw coffee bean extract) and polyphenol oxidase (gobo extract or prune extract). You can check it.
Above all, it can be confirmed that when 1 g and 2 g of Samples P1 were ingested in Examples 1 and 3, that is, diallyl disulfide and allyl methyl sulfide were remarkably suppressed in any time zone. On the other hand, although the inhibitory effect was also shown in Example 5, that is, when 0.5 g of sample P1 was ingested, the effect was weak as compared with Examples 1 and 3. In Example 4, that is, when 4 g of sample P1 was ingested, only diallyl disulfide was suppressed.
From Example 2, it was confirmed that the emission of diallyl disulfide and allyl methyl sulfide was remarkably suppressed by using the polyphenol oxidase derived from the prune extract in combination with polyphenol.
Therefore, ingestion of polyphenolic substances and polyphenol oxidase is considered to be particularly effective in suppressing body odor. It can also be confirmed that the inhibitory effect is already exhibited 30 minutes after ingesting garlic.
From Table 2, what is particularly effective in suppressing body odor is a deodorant composition containing 0.21 mmol to 1.69 mmol of polyphenol in terms of catechol and polyphenol oxidase of 12.5 Units or more, and more preferably in terms of catechol. It can be seen that the deodorant composition contains 0.42 mmol to 0.85 mmol of polyphenols and 15 Units to 50 Units of polyphenol oxidase.
In addition, from the results of Comparative Examples 2 and 3, it was confirmed that the emission of diallyl disulfide and allyl methyl sulfide was not suppressed by ingestion of only the polyphenolic substance or the polyphenol oxidase. Therefore, it was found that oral ingestion of a polyphenolic substance and a polyphenol oxidase in combination is effective in suppressing body odor.

3. 3. Body odor suppression test procedure Each sample (mixture of green coffee bean extract and burdock extract, and mixture of raw coffee bean extract and prune extract) was ingested by the subjects to evaluate the body odor suppression effect.
Specifically, first, two adult males and one female subject were ingested 140 g of bonito sashimi from 11:00 am. Five minutes after the bonito was ingested, each sample was dissolved in 200 ml of water and ingested. Then, skin gas was collected over time before and after ingestion of bonito. The test was conducted on the same day for all three subjects. Since the test results showed the same tendency in all three subjects, the results of one of the subjects are described as an example.
The skin gas was collected from a predetermined position on the left forearm by a skin gas collecting device (described in Japanese Patent No. 4654045) using an ammonia collecting filter as a collecting agent. First, as skin gas before ingestion of bonito, 1 hour from 9 am to 10 am and 1 hour from 10 am to 11 am were collected. The measurement of skin gas after ingestion of bonito was started at 11:30 am, and collection was carried out at 1-hour intervals from 11:30 am to 15:30 am.
After collection, the amount of ammonium ions in the extract from which the collection filter was extracted was measured by an ion chromatograph (manufactured by Shimadzu Corporation) to determine the emission flux (ngcm ^{- 2h-1} ). The formula for calculating the emission flux is the same as that described above.

[Example 6]
Five minutes after completely ingesting 140 g of bonito sashimi, 1 g of sample P1 (1: 1 mixture of raw coffee bean extract and burdock extract prepared in Reference Example 3 (1)) was dissolved in 200 ml of water. Ingested.

[Example 7]
Five minutes after ingesting 140 g of bonito sashimi, 1 g of sample P2 (1: 1 mixture of raw coffee bean extract and prune extract prepared in Reference Example 3 (2)) was dissolved in 200 ml of water and ingested.

[Comparative Example 4]
Five minutes after ingesting 140 g of bonito sashimi, 200 ml of water was ingested.

The measurement results of ammonia in Example 6 and Comparative Example 4 are shown in FIG.
The measurement results of ammonia in Example 7 and Comparative Example 4 are shown in FIG.

As shown in FIGS. 13 and 14, the amount of ammonia emitted is reduced by ingestion of a mixture of a polyphenolic substance (raw coffee bean extract) and a polyphenol oxidase (gobo extract or prune extract). You can check it.

From the results of the above examples, it was found that the deodorant composition of the present invention can control the body odor generated from the inside of the body. Although either polyphenol or polyphenol oxidase alone is not effective in suppressing body odor, oral ingestion of a deodorant composition combining both is effective in suppressing body odor. As described above, since the deodorant composition of the present invention is composed of naturally occurring polyphenols and polyphenol oxidases, it can be ingested orally, and is effective in suppressing body odor generated from the inside of the body and emitted through the skin. It was shown to be.

Although the present invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention. This application is based on a Japanese patent application filed on August 9, 2018 (Japanese Patent Application No. 2018-150820), the contents of which are incorporated herein by reference.

The present invention provides an orally ingestible deodorant composition and a method for suppressing body odor, which have a deodorizing effect not only on halitosis and fecal odor but also on body odor emitted from the body.

Claims (5)

A deodorant composition that suppresses body odor derived from the body by ingestion, and is a deodorant composition containing polyphenols and polyphenol oxidase. The deodorant composition according to claim 1, wherein the polyphenol contains at least one of an o-diphenol structure and a p-diphenol structure. The deodorant composition according to claim 1 or 2, wherein the polyphenol oxidase is an enzyme obtained from a plant extract. The deodorant composition according to any one of claims 1 to 3, wherein the content of polyphenol is 2 mmol or less in terms of catechol. A method for suppressing body odor derived from the body by orally ingesting the deodorant composition according to any one of claims 1 to 4.

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