JPWO2020028648A5 - - Google Patents

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JPWO2020028648A5
JPWO2020028648A5 JP2021529243A JP2021529243A JPWO2020028648A5 JP WO2020028648 A5 JPWO2020028648 A5 JP WO2020028648A5 JP 2021529243 A JP2021529243 A JP 2021529243A JP 2021529243 A JP2021529243 A JP 2021529243A JP WO2020028648 A5 JPWO2020028648 A5 JP WO2020028648A5
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focal plane
image
image object
images
optimal
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JP7166456B2 (en
JP2021533389A (en
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Claims (6)

生体試料の合成デジタル画像を生成するシステムであって、
前記生体試料の複数の画像から前記生体試料の合成画像を生成するように構成されたコンピュータ装置を含み、前記複数の画像の各々は、単一の軸に沿って撮影され、
前記コンピュータ装置は、
（ａ）前記生体試料の第１のｘ－ｙロケーションにおける画像物体の第１の集合の、第１のｚ距離を含む第１の焦点面を識別することと、
（ｂ）前記生体試料の第２のｘ－ｙロケーションにおける画像物体の第２の集合の、第２のｚ距離を含む第２の焦点面を識別することと、
（ｃ）前記（ａ）及び前記（ｂ）にそれぞれ基づいて、第１の最適な焦点面及び第２の最適な焦点面を識別することと、
（ｄ）第１の最適な焦点面からの画像物体及び第２の最適な焦点面からの画像物体を結合し、且つ前記合成デジタル画像を生成することと
を行い、
前記コンピュータ装置は、前記第１の焦点面における前記画像物体の鮮鋭値を計算し、前記第２の焦点面における前記画像物体の鮮鋭値を計算し、最高鮮鋭値を有する前記画像物体のロケーションの二次元マップを生成し、
前記コンピュータ装置は、前記第１及び第２の焦点面において前記最高鮮鋭値を有する前記画像物体の前記ロケーションの前記二次元マップに対して１つ又は複数の膨張及び収縮を実行することにより、第１及び第２の画像物体の集合の最適な第１の焦点面及び最適な第２の焦点面を識別し、
前記膨張及び収縮の数は、健康な組織における細胞境界間の距離に比例し、それによって前記第１及び前記第２の最適な焦点面を識別し、
前記第１の最適な焦点面に配置された画像物体は、ピントが合った状態で提示され、及び前記第１の最適な焦点面の下の画像物体は、強調抑制され、
前記第２の最適な焦点面に配置された画像物体は、ピントが合った状態で提示され、及び前記第２の最適な焦点面の下の画像物体は、強調抑制される、システム。 A system that generates synthetic digital images of biological samples.
A computer device configured to generate a composite image of the biological sample from a plurality of images of the biological sample is included, each of the plurality of images being taken along a single axis.
The computer device is
(A) Identifying the first focal plane of the first set of image objects at the first xy location of the biological sample , including the first z distance .
(B) Identifying the second focal plane of the second set of image objects at the second xy location of the biological sample , including the second z distance .
(C) Identifying the first optimum focal plane and the second optimum focal plane based on the above (a) and (b), respectively.
( D) The image object from the first optimum focal plane and the image object from the second optimum focal plane are combined to generate the synthetic digital image.
The computer device calculates the sharpness value of the image object on the first focal plane, calculates the sharpness value of the image object on the second focal plane, and determines the location of the image object having the highest sharpness value. Generate a two-dimensional map and
The computer device performs one or more expansions and contractions with respect to the two-dimensional map of the location of the image object having the sharpest value in the first and second focal planes. Identify the optimal first focal plane and the optimal second focal plane of the set of first and second image objects.
The number of expansions and contractions is proportional to the distance between cell boundaries in healthy tissue, thereby identifying the first and second optimal focal planes.
The image object placed on the first optimal focal plane is presented in focus, and the image object below the first optimal focal plane is enhanced and suppressed.
An image object placed on the second optimal focal plane is presented in focus, and an image object below the second optimal focal plane is enhanced and suppressed. 前記複数の画像における前記画像の１つ又は複数は、カラー画像であり、及び前記コンピュータ装置は、前記複数の画像の最適な焦点面を識別する前に、前記複数の画像における前記１つ又は複数のカラー画像をグレースケールに変換する、請求項１に記載のシステム。 One or more of the images in the plurality of images is a color image, and the computer apparatus said one or more in the plurality of images before identifying the optimal focal plane of the plurality of images. The system according to claim 1, wherein the color image of the above is converted into grayscale. 前記合成画像は、前記生体試料のハニカム構造を含む、請求項１に記載のシステム。 The system according to claim 1, wherein the synthetic image includes a honeycomb structure of the biological sample. 前記ハニカム構造は、実質的にピントが合っている、請求項３に記載のシステム。 The system according to claim 3 , wherein the honeycomb structure is substantially in focus. 顕微鏡及びカメラを更に含む、請求項１に記載のシステム。 The system of claim 1, further comprising a microscope and a camera. 顕微鏡ステージを更に含む、請求項５に記載のシステム。 The system of claim 5 , further comprising a microscope stage.
JP2021529243A 2018-08-01 2019-08-01 Enhanced depth of focus for biological samples Active JP7166456B2 (en)

Priority Applications (1)

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JP2022170598A JP7244706B2 (en) 2018-08-01 2022-10-25 A system for generating synthetic digital images of biological specimens

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US201862713076P 2018-08-01 2018-08-01
US62/713,076 2018-08-01
PCT/US2019/044639 WO2020028648A1 (en) 2018-08-01 2019-08-01 Enhanced extended depth of focusing on biological samples

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JP2021533389A JP2021533389A (en) 2021-12-02
JPWO2020028648A5 true JPWO2020028648A5 (en) 2022-05-30
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IL280503B (en) * 2018-08-01 2022-09-01 Cdx Medical Ip Inc Enhanced extended depth of focusing on biological samples

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