JPWO2019189343A1 - Liquid container and nucleic acid separator containing it - Google Patents

Liquid container and nucleic acid separator containing it Download PDF

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JPWO2019189343A1
JPWO2019189343A1 JP2020509184A JP2020509184A JPWO2019189343A1 JP WO2019189343 A1 JPWO2019189343 A1 JP WO2019189343A1 JP 2020509184 A JP2020509184 A JP 2020509184A JP 2020509184 A JP2020509184 A JP 2020509184A JP WO2019189343 A1 JPWO2019189343 A1 JP WO2019189343A1
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liquid
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nucleic acid
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基範 岡島
基範 岡島
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Kaneka Corp
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology

Abstract

本発明は、蛇腹部の圧縮変形により液体を放出する液体収容容器において、放出時の液体の残留を低減することを目的とする。本発明の液体収容容器１０００は、第１空間１１０１を内包し、第１空間１１０１の体積が減少するように１つの軸Ｘに沿って圧縮変形可能な蛇腹部１１２０と、蛇腹部１１２０の一端に接続され、液体ＬＡを収容するための第２空間１１０２を内包し、液体ＬＡを放出するための放出口１１３１が形成された液体収容部１１３０と、第２空間１１０２から第１空間１１０１への液体ＬＡの流入を妨げ、且つ、蛇腹部１１２０を圧縮変形させたときの第１空間１１０１から第２空間１１０２への気体の流入を許容する逆止部１１４０とを含む容器本体１１００、並びに、第２空間１１０２に収容された液体ＬＡを備えることを特徴とする。An object of the present invention is to reduce residual liquid at the time of discharge in a liquid storage container that discharges liquid by compressive deformation of the bellows portion. The liquid storage container 1000 of the present invention contains the first space 1101 and is formed on a bellows portion 1120 that can be compressed and deformed along one axis X so that the volume of the first space 1101 is reduced, and at one end of the bellows portion 1120. A liquid accommodating portion 1130 which is connected and contains a second space 1102 for accommodating the liquid LA and has a discharge port 1131 for discharging the liquid LA, and a liquid from the second space 1102 to the first space 1101. The container body 1100 including the non-stop portion 1140 that prevents the inflow of LA and allows the inflow of gas from the first space 1101 to the second space 1102 when the bellows portion 1120 is compressed and deformed, and the second It is characterized by including a liquid LA housed in the space 1102.

Description

本発明は、液体を収容し放出する液体収容容器及びそれを含む核酸分離装置に関する。 The present invention relates to a liquid storage container for storing and discharging a liquid and a nucleic acid separation device containing the same.

遺伝子検査は核酸を解析することにより高感度かつ短時間での結果判定が可能であり、農畜水産、品質検査など多様な領域において応用される産業上重要な検査法である。遺伝子検査のためには、核酸を含む検体から、核酸を分離する必要がある。 Genetic testing can determine the results with high sensitivity and in a short time by analyzing nucleic acids, and is an industrially important testing method applied in various fields such as agriculture, livestock and fisheries, and quality testing. For genetic testing, it is necessary to separate nucleic acids from samples containing nucleic acids.

核酸を含む検体から核酸を分離する方法としては、核酸を含む検体から調製された、核酸を含む液体を、核酸が吸着する液透過性担体に通過させた後、該担体に吸着された核酸を回収する方法や、核酸を含む液体を、核酸が透過する液透過性担体に通過させて該担体に不純物を吸着し、該担体を透過した、核酸を含む液体を回収する方法が知られている。 As a method for separating nucleic acid from a sample containing nucleic acid, a liquid containing nucleic acid prepared from a sample containing nucleic acid is passed through a liquid-permeable carrier on which the nucleic acid is adsorbed, and then the nucleic acid adsorbed on the carrier is passed. Known methods are a method of recovering, or a method of passing a liquid containing nucleic acid through a liquid-permeable carrier through which nucleic acid permeates, adsorbing impurities on the carrier, and recovering the liquid containing nucleic acid that has permeated the carrier. ..

特許文献１では、核酸処理試薬を収容し、圧縮変形により体積が減少可能に構成された処理試薬収容容器と、核酸を含む検体を収容するための収容空間を有し、核酸吸着担体を備えた核酸採取部材とを用意し、前記核酸採取部材に核酸を含む検体を収容した後、前記核酸採取部材に前記処理試薬収容容器を接続して一体化し、一体化された容器内で前記核酸処理試薬と前記検体との混合液を生成した後、前記処理試薬収容容器を圧縮変形させて体積を減少させ、前記混合液を前記核酸吸着担体に透過させて排出する方法が記載されている。前記処理試薬収容容器の、圧縮変形により体積が減少可能な構造として蛇腹構造が開示されている。 In Patent Document 1, a processing reagent storage container configured to store a nucleic acid processing reagent and whose volume can be reduced by compression deformation, a storage space for storing a sample containing nucleic acid, and a nucleic acid adsorption carrier are provided. After preparing a nucleic acid collecting member and accommodating a sample containing nucleic acid in the nucleic acid collecting member, the processing reagent accommodating container is connected to the nucleic acid collecting member and integrated, and the nucleic acid processing reagent is integrated in the integrated container. A method is described in which after producing a mixed solution of the nucleic acid and the sample, the processing reagent storage container is compressed and deformed to reduce the volume, and the mixed solution is permeated through the nucleic acid adsorption carrier and discharged. A bellows structure is disclosed as a structure in which the volume of the processing reagent container can be reduced by compression deformation.

特許文献２では、生体物質等の各種物質が結合されまたは結合可能な担体を封入し、担体による処理を容易化した担体封入変形容器が開示されている。前記担体封入変形容器は、壁面で囲まれた内部に液体および気体を収容可能であって、その壁面の全内表面積を実質的に変えることなく所定の変形が可能な変形壁面を前記壁面の一部に有する収容部と、該収容部と連通し該変形壁面の変形による前記内部の膨張および収縮によって吸引吐出される液体が流入流出可能な口部と、前記収容部内に略静止状態で封入された所定の物質が結合しまたは結合可能な担体とを有する。特許文献２では、前記変形壁面として蛇腹構造の壁面が開示されている。 Patent Document 2 discloses a carrier-encapsulated deformable container in which a carrier to which various substances such as biological substances can be bound or can be bound is sealed to facilitate treatment with the carrier. The carrier-encapsulated deformable container has a deformed wall surface that can contain liquids and gases inside the wall surface and can be deformed in a predetermined manner without substantially changing the total internal surface area of the wall surface. The accommodating portion provided in the accommodating portion, the mouth portion through which the liquid sucked and discharged by the expansion and contraction of the inside due to the deformation of the deformed wall surface can flow in and out, and the accommodating portion are enclosed in the accommodating portion in a substantially stationary state. It has a carrier to which a predetermined substance can be bound or bound. Patent Document 2 discloses a wall surface having a bellows structure as the deformed wall surface.

国際公開ＷＯ２０１７／０４３６４９International release WO2017 / 043649 特開２０１２−１５０１１８号公報Japanese Unexamined Patent Publication No. 2012-150118

特許文献１及び２には、核酸を処理するための薬液等の液体を収容する容器の壁面の一部が蛇腹部により構成されており、該蛇腹部を圧縮変形することで容器内空間を減少させ、液体を放出する容器が記載されている。 In Patent Documents 1 and 2, a part of the wall surface of a container for accommodating a liquid such as a chemical solution for processing nucleic acid is composed of a bellows portion, and the space inside the container is reduced by compressing and deforming the bellows portion. A container that allows the liquid to be discharged is described.

蛇腹部は、１つの軸に沿って圧縮変形可能なように、該軸に略垂直な方向に延びる山及び谷が、該軸の方向に交互に複数形成された、折れ曲がり可能な壁面からなる部分である。蛇腹部を備えた容器を用意し、蛇腹部を軸に沿って圧縮変形させるとき、蛇腹部を構成する壁面が折れ曲がり、図７Ｃに示すように、容器内から見た時の個々の谷を構成する一対の壁面間の前記軸の方向の距離が縮まる。前記一対の壁面同士が密着する場合もある。このようにして、蛇腹部の圧縮変形により容器内に幅の狭い複数の谷が形成される。 The bellows portion is a portion consisting of a bendable wall surface in which a plurality of peaks and valleys extending in a direction substantially perpendicular to the axis are alternately formed in the direction of the axis so that the bellows portion can be compressed and deformed along one axis. Is. When a container equipped with a bellows portion is prepared and the bellows portion is compressed and deformed along an axis, the wall surface constituting the bellows portion is bent, and as shown in FIG. 7C, individual valleys when viewed from the inside of the container are formed. The distance in the direction of the axis between the pair of wall surfaces is reduced. The pair of wall surfaces may be in close contact with each other. In this way, the compression deformation of the bellows portion forms a plurality of narrow valleys in the container.

本発明者らは、特許文献１及び２に記載されているような、液体を収容し、該液体と直接接触する壁部に蛇腹部が形成されている容器では、液体を放出するために蛇腹部を圧縮変形させた時に、容器内の壁面に形成された幅の狭い複数の谷の部分に、放出されるべき液体が入り込み、容器から放出されずに容器内に残留してしまうという課題を見出した。液体が容器内に残留すると、規定量の液体が放出されないという問題がある。特に、容器内の液体が、分析等で利用しようとする微量の成分（例えば核酸）を含む場合には、該成分を含む液体を容器から回収できないことの不利益が大きい。 The present inventors, as described in Patent Documents 1 and 2, in a container containing a liquid and having a bellows portion formed on a wall portion in direct contact with the liquid, bellows to release the liquid. When the portion is compressed and deformed, the problem is that the liquid to be released enters the narrow valleys formed on the wall surface of the container and remains in the container without being released from the container. I found it. If the liquid remains in the container, there is a problem that the specified amount of liquid is not released. In particular, when the liquid in the container contains a trace amount of a component (for example, nucleic acid) to be used for analysis or the like, there is a great disadvantage that the liquid containing the component cannot be recovered from the container.

本発明者らは、蛇腹部の圧縮変形により液体を放出する液体収容容器において、放出時の液体の残留を低減することを目的として鋭意検討し以下の本発明を完成するに至った。
（１）液体を収容し放出する液体収容容器であって、
第１空間を内包し、前記第１空間の体積が減少するように１つの軸に沿って圧縮変形可能な蛇腹部と、
前記蛇腹部の一端に接続され、液体を収容するための第２空間を内包し、液体を放出するための放出口が形成された液体収容部と、
前記第２空間から前記第１空間への液体の流入を妨げ、且つ、前記蛇腹部を圧縮変形させたときの前記第１空間から前記第２空間への気体の流入を許容する逆止部と
を含む容器本体
を備える液体収容容器。
（２）前記逆止部が、前記第１空間と前記第２空間とを接続する貫通孔を有する、（１）に記載の液体収容容器。
（３）前記貫通孔の最狭部の幅が０．５ｍｍ以下である、（２）に記載の液体収容容器。
（４）前記貫通孔の長さが０．１ｍｍ以上である、（２）又は（３）に記載の液体収容容器。
（５）前記貫通孔を閉塞し、前記蛇腹部を圧縮変形させたときに前記貫通孔を開放する閉塞部を更に含む、（２）〜（４）のいずれかに記載の液体収容容器。
（６）前記逆止部が多孔質体を含む、（１）に記載の液体収容容器。
（７）前記逆止部が逆止弁である、（１）に記載の液体収容容器。
（８）前記第２空間に収容された液体を更に備える、（１）〜（７）のいずれかに記載の液体収容容器。
（９）前記放出口を封止する蓋体を更に備える、（１）〜（８）のいずれかに記載の液体収容容器。
（１０）前記第２空間に収容された液体を更に備え、
前記液体が、核酸、核酸を含有する検体、又は核酸を担持した担体を処理するための薬液である、（１）〜（９）のいずれかに記載の液体収容容器。The present inventors have earnestly studied for the purpose of reducing the residual liquid at the time of discharge in a liquid storage container that discharges a liquid by compressive deformation of the bellows portion, and have completed the following invention.
(1) A liquid storage container that stores and discharges liquid.
A bellows portion that includes the first space and can be compressed and deformed along one axis so that the volume of the first space is reduced.
A liquid accommodating portion connected to one end of the bellows portion, containing a second space for accommodating the liquid, and forming a discharge port for discharging the liquid.
A check valve that prevents the inflow of liquid from the second space into the first space and allows the inflow of gas from the first space to the second space when the bellows portion is compressed and deformed. A liquid storage container comprising a container body containing.
(2) The liquid storage container according to (1), wherein the check valve has a through hole connecting the first space and the second space.
(3) The liquid storage container according to (2), wherein the width of the narrowest portion of the through hole is 0.5 mm or less.
(4) The liquid storage container according to (2) or (3), wherein the length of the through hole is 0.1 mm or more.
(5) The liquid storage container according to any one of (2) to (4), further including a closed portion that closes the through hole and opens the through hole when the bellows portion is compressively deformed.
(6) The liquid storage container according to (1), wherein the check valve contains a porous body.
(7) The liquid storage container according to (1), wherein the check valve is a check valve.
(8) The liquid storage container according to any one of (1) to (7), further comprising a liquid stored in the second space.
(9) The liquid storage container according to any one of (1) to (8), further comprising a lid for sealing the discharge port.
(10) Further provided with the liquid contained in the second space,
The liquid storage container according to any one of (1) to (9), wherein the liquid is a nucleic acid, a sample containing nucleic acid, or a chemical solution for treating a carrier carrying nucleic acid.

（１１）前記放出口を封止する蓋体を更に備える、（１０）に記載の液体収容容器と、
核酸を含む検体を収容するための検体収容空間を内包し、前記検体収容空間の一方側に、前記薬液が供給可能なように前記液体収容容器の前記放出口に接続される薬液供給口が形成されており、前記検体収容空間の他方側に、前記薬液と前記検体との混合物を、前記混合物中の核酸を吸着する又は核酸を通過させる液透過性担体を介して排出する排出口が形成されている担体保持部と、
を備え、
前記担体保持部は、前記薬液供給口に前記液体収容容器を接続する際に、前記液体収容容器の、前記蓋体による前記放出口の封止を開放し、前記放出口と前記薬液供給口とを連通する、開放部を備える、核酸分離装置。(11) The liquid storage container according to (10), further comprising a lid for sealing the discharge port.
A sample storage space for storing a sample containing nucleic acid is included, and a drug solution supply port connected to the discharge port of the liquid storage container is formed on one side of the sample storage space so that the drug solution can be supplied. On the other side of the sample storage space, a discharge port is formed for discharging the mixture of the drug solution and the sample via a liquid-permeable carrier that adsorbs the nucleic acid in the mixture or allows the nucleic acid to pass through. Carrier holder and
With
When the liquid storage container is connected to the chemical solution supply port, the carrier holding portion opens the seal of the discharge port of the liquid storage container by the lid body, and the discharge port and the chemical solution supply port A nucleic acid separator having an open portion for communicating with each other.

本明細書は本願の優先権の基礎となる日本国特許出願番号２０１８−０６６９９２号の開示内容を包含する。 This specification includes the disclosure content of Japanese Patent Application No. 2018-066992, which is the basis of the priority of the present application.

本発明に係る液体収容容器によれば、蛇腹部を圧縮変形して液体を放出する際の液体の残留が少ない。 According to the liquid container according to the present invention, there is little residual liquid when the bellows portion is compressed and deformed to release the liquid.

本発明に係る核酸分離装置によれば、蛇腹部を圧縮変形して薬液を圧送して液透過性担体に透過させる際の、薬液の残留が少ない。 According to the nucleic acid separating apparatus according to the present invention, there is little residual chemical solution when the bellows portion is compressed and deformed and the chemical solution is pumped to permeate through the liquid permeable carrier.

図１はロック部２０の外観を示す。FIG. 1 shows the appearance of the lock portion 20. 図２は液体収容容器１０００の正面図である。FIG. 2 is a front view of the liquid storage container 1000. 図３Ａは、液体収容容器１０００とロック部２０とを一体化したユニット２の正面図である。FIG. 3A is a front view of the unit 2 in which the liquid storage container 1000 and the lock portion 20 are integrated. 図３Ｂは、ユニット２の断面図である。FIG. 3B is a cross-sectional view of the unit 2. 図４Ａは、担体保持部の、検体を採取するための第１部材１３１０の正面図である。FIG. 4A is a front view of the carrier holding portion of the first member 1310 for collecting a sample. 図４Ｂは、担体保持部の、検体を採取するための第１部材１３１０の上面図である。FIG. 4B is a top view of the carrier holding portion of the first member 1310 for collecting a sample. 図４Ｃは、図４ＢでのＡ−Ａ線による第１部材１３１０の断面図である。FIG. 4C is a cross-sectional view of the first member 1310 taken along the line AA in FIG. 4B. 図５Ａは、担体保持部の、担体１３を保持する第２部材１３２０の正面図である。FIG. 5A is a front view of the carrier holding portion of the second member 1320 that holds the carrier 13. 図５Ｂは、担体保持部の、担体１３を保持する第２部材１３２０の上面図である。FIG. 5B is a top view of the carrier holding portion of the second member 1320 that holds the carrier 13. 図５Ｃは、図５ＢでのＡ−Ａ線による第２部材１３２０断面図である。FIG. 5C is a cross-sectional view of the second member 1320 taken along the line AA in FIG. 5B. 図６Ａは、第１部材１３１０と、第２部材１３２０と、担体１３を組み合わせて構成された担体保持部１２０の断面図である。FIG. 6A is a cross-sectional view of a carrier holding portion 120 formed by combining the first member 1310, the second member 1320, and the carrier 13. 図６Ｂは、担体保持部１２０の検体収容空間１３１に、核酸を含む検体Ｓを収容した状態の断面図である。FIG. 6B is a cross-sectional view of a sample S containing nucleic acid in the sample storage space 131 of the carrier holding unit 120. 図７Ａは、核酸分離装置１の正面図である。FIG. 7A is a front view of the nucleic acid separation device 1. 図７Ｂは、核酸分離装置１の断面図である。FIG. 7B is a cross-sectional view of the nucleic acid separation device 1. 図７Ｃは、核酸分離装置１において、蛇腹部１１２０を圧縮変形させ、ロック部２０により変形状態を保持したときの、圧縮直後の状態の断面図である。FIG. 7C is a cross-sectional view of the state immediately after compression when the bellows portion 1120 is compression-deformed and the deformed state is held by the lock portion 20 in the nucleic acid separation device 1. 図７Ｄは、核酸分離装置１において、蛇腹部１１２０を圧縮変形させ、ロック部２０により変形状態を保持し、液体Ｌの全量が担体１３を透過した状態の断面図である。FIG. 7D is a cross-sectional view of the nucleic acid separation device 1 in which the bellows portion 1120 is compressionally deformed, the deformed state is held by the lock portion 20, and the entire amount of the liquid L has passed through the carrier 13. 図８は、図７Ｄに示す液体Ｌ透過後の核酸分離装置１から、担体保持部１２０の第１部材１３１０よりも上流側を取り外し、核酸を担持している担体１３を含む第２部材１３２０と回収容器４０を残した状態の断面図である。FIG. 8 shows the second member 1320 containing the carrier 13 carrying the nucleic acid by removing the upstream side of the carrier holding portion 120 from the first member 1310 from the nucleic acid separating device 1 after the liquid L permeation shown in FIG. 7D. It is sectional drawing of the state which left the recovery container 40. 図９は、核酸を担持している担体１３を洗浄するための洗浄液ＬＢを収容した洗浄液収容容器２０００及びロック部２０を備えたユニット３の断面図である。Figure 9 is a cross-sectional view of the unit 3 with a cleaning liquid container 2000 and a lock portion 20 accommodating the cleaning liquid L B for washing the carrier 13 carrying the nucleic acid. 図１０Ａは、洗浄液収容容器２０００及びロック部２０を備えたユニット２を、核酸を担持している担体１３を含む第２部材１３２０に接続して形成した装置４の断面図である。FIG. 10A is a cross-sectional view of an apparatus 4 formed by connecting a unit 2 provided with a cleaning liquid storage container 2000 and a lock portion 20 to a second member 1320 including a carrier 13 carrying a nucleic acid. 図１０Ｂは、装置４において、蛇腹部１１２０を圧縮変形させ、ロック部２０により変形状態を保持し、洗浄液ＬＢが担体１３を透過した状態の断面図である。Figure 10B is the device 4, the bellows portion 1120 is compressed and deformed to hold the deformed state by the lock portion 20 is a sectional view of a state in which the cleaning liquid L B is transmitted through the carrier 13. 図１１は、洗浄液ＬＢによる洗浄後の、核酸を担持した担体１３を保持する第２部材１３２０の断面図である。Figure 11 is a cross-sectional view of the second member 1320 to hold after washing with the washing liquid L B, a carrier 13 which nucleic acid carrying. 図１２は、シリンジ５０から溶離液ＬＣを、核酸を担持した担体１３に向けて注入して通過させ、核酸を含む溶離液ＬＣを試料採取容器６０に採取する工程を説明するための模式図である。Figure 12 is an eluent L C from the syringe 50, the nucleic acid is injected toward the carrier 13 which carries passed through a schematic for the eluent L C illustrating the process of collecting a sample collection container 60 containing a nucleic acid It is a figure. 図１３Ａは、貫通孔１１４１を有する逆止部１１４０の斜視図である。FIG. 13A is a perspective view of the check valve 1140 having the through hole 1141. 図１３Ｂは、貫通孔１１４１を有する逆止部１１４０の断面図である。FIG. 13B is a cross-sectional view of the check valve 1140 having the through hole 1141. 図１４は、貫通孔１１４１を閉塞し、蛇腹部１１２０を圧縮変形させたときに貫通孔１１４１を開放する閉塞部１１４７を更に有する逆止部１１４０を備えた液体収容容器１０００と、ロック部２０とを組み合わせたユニット２の断面図である。FIG. 14 shows a liquid storage container 1000 having a non-stop portion 1140 further having a closing portion 1147 that closes the through hole 1141 and opens the through hole 1141 when the bellows portion 1120 is compressively deformed, and a lock portion 20. It is sectional drawing of the unit 2 which combined. 図１５は、多孔質体１１５１を含む逆止部１１５０を備えた液体収容容器１０００と、ロック部２０とを組み合わせたユニット２の断面図である。FIG. 15 is a cross-sectional view of a unit 2 in which a liquid storage container 1000 having a check valve portion 1150 including a porous body 1151 and a lock portion 20 are combined. 図１６は、逆止弁である逆止部１１６０を備えた液体収容容器１０００と、ロック部２０とを組み合わせたユニット２の断面図である。FIG. 16 is a cross-sectional view of a unit 2 in which a liquid storage container 1000 provided with a check valve 1160 and a lock portion 20 are combined.

＜液体収容容器＞
本発明の液体収容容器の一実施形態として、図２に示す液体収容容器１０００について説明する。図２は、液体収容容器１０００の正面図である。図１はロック部２０の外観を示す。図３Ａは、液体収容容器１０００と、ロック部２０とを一体化したユニット２の正面図であり、図３Ｂは、ユニット２の断面図である。<Liquid storage container>
As an embodiment of the liquid storage container of the present invention, the liquid storage container 1000 shown in FIG. 2 will be described. FIG. 2 is a front view of the liquid storage container 1000. FIG. 1 shows the appearance of the lock portion 20. FIG. 3A is a front view of the unit 2 in which the liquid storage container 1000 and the lock portion 20 are integrated, and FIG. 3B is a cross-sectional view of the unit 2.

本実施形態の液体収容容器１０００は、
第１空間１１０１を内包し、第１空間１１０１の体積が減少するように１つの軸Ｘに沿って圧縮変形可能な蛇腹部１１２０と、
蛇腹部１１２０の一端に接続され、液体ＬＡを収容するための第２空間１１０２を内包し、液体ＬＡを放出するための放出口１１３１が形成された液体収容部１１３０と、
第２空間１１０２から第１空間１１０１への液体ＬＡの流入を妨げ、且つ、蛇腹部１１２０を圧縮変形させたときの第１空間１１０１から第２空間１１０２への気体の流入を許容する逆止部１１４０と
を含む容器本体１１００、
第２空間１１０２に収容された液体ＬＡ、並びに、
放出口１１３１を封止する蓋体１２００
を備える。The liquid storage container 1000 of the present embodiment is
A bellows portion 1120 that includes the first space 1101 and can be compressed and deformed along one axis X so that the volume of the first space 1101 is reduced.
Is connected to one end of the bellows portion 1120, a second space 1102 for accommodating the liquid L A encloses a liquid storage portion 1130 outlet 1131 is formed for releasing liquid L A,
A non- stop that prevents the inflow of liquid LA from the second space 1102 to the first space 1101 and allows the inflow of gas from the first space 1101 to the second space 1102 when the bellows portion 1120 is compressed and deformed. Container body 1100, including part 1140,
Liquid L A, which is accommodated in the second space 1102, and,
Lid body 1200 that seals the discharge port 1131
To be equipped.

本発明において「蛇腹部」とは、容器本体の壁面の一部であって、軸Ｘに沿って圧縮変形可能であり、軸Ｘに略垂直な方向に、軸Ｘの周りを囲うように延びる山及び谷が、軸Ｘの方向に交互に複数形成された折れ曲がり可能な壁面からなる部分である。蛇腹部は、典型的には、軸Ｘの方向に延び一端が閉塞し他端が開放された筒状の部分である。図示する実施形態では、蛇腹部は、閉塞した前記一端の側から押圧して圧縮変形されることから、前記一端を「押圧部」と称する。 In the present invention, the "bellows portion" is a part of the wall surface of the container body, is deformable by compression along the axis X, and extends so as to surround the axis X in a direction substantially perpendicular to the axis X. A portion consisting of a plurality of bendable wall surfaces formed alternately in the direction of the axis X, with peaks and valleys. The bellows portion is typically a tubular portion that extends in the direction of the axis X, with one end closed and the other end open. In the illustrated embodiment, the bellows portion is compressed and deformed by being pressed from the side of the closed one end, and therefore the one end is referred to as a “pressing portion”.

本実施形態の液体収容容器１０００において蛇腹部１１２０は、軸Ｘに垂直な平面よる断面が円形であるが、該断面の形状は限定されず、四角形（正方形、長方形）、三角形、五角形等の多角形であってもよい。円形には、真円、楕円、真円が扁平した形状、楕円が変形した形状などが含まれる。多角形には角部が丸みを帯びた形状も含まれる。 In the liquid storage container 1000 of the present embodiment, the bellows portion 1120 has a circular cross section along a plane perpendicular to the axis X, but the shape of the cross section is not limited, and there are many quadrangles (squares, rectangles), triangles, pentagons, and the like. It may be rectangular. The circular shape includes a perfect circle, an ellipse, a flat shape of the perfect circle, and a deformed shape of the ellipse. Polygons also include rounded corners.

本実施形態の液体収容容器１０００において液体収容部１１３０は、蛇腹部１１２０の一方の開放された端部の側に配置された、放出口１１３１を囲う部分である。本実施形態の液体収容容器１０００において、蛇腹部１１２０と液体収容部１１３０とは、接続部１１８０により接続されている。本実施形態では、液体収容部１１３０は筒状であり、一方側が開放されて放出口１１３１を形成し、他方側が、接続部１１８０を介して蛇腹部１１２０に接続される。液体収容部１１３０の軸Ｘと垂直な方向の幅は、接続部１１８０の軸Ｘと垂直な方向の幅よりも大きく、液体収容部１１３０と接続部１１８０とは段部１１８１を介して接続されている。 In the liquid storage container 1000 of the present embodiment, the liquid storage portion 1130 is a portion surrounding the discharge port 1131 arranged on the side of one open end of the bellows portion 1120. In the liquid storage container 1000 of the present embodiment, the bellows portion 1120 and the liquid storage portion 1130 are connected by a connecting portion 1180. In the present embodiment, the liquid accommodating portion 1130 has a tubular shape, one side is opened to form a discharge port 1131, and the other side is connected to the bellows portion 1120 via the connecting portion 1180. The width of the liquid accommodating portion 1130 in the direction perpendicular to the axis X is larger than the width of the connecting portion 1180 in the direction perpendicular to the axis X, and the liquid accommodating portion 1130 and the connecting portion 1180 are connected via the step portion 1181. There is.

容器本体１１００において、押圧部１１２１と放出口１１３１は、蛇腹部１１２０の軸Ｘの方向に対向する位置に配置されている。 In the container body 1100, the pressing portion 1121 and the discharge port 1131 are arranged at positions facing each other in the direction of the axis X of the bellows portion 1120.

容器本体１１００の液体収容部１１３０における放出口１１３１を封止する蓋体１２００は、容易に破壊されるように構成されている。蓋体１２００は合成樹脂を含むフィルムや、アルミニウムフィルムなどの金属フィルムにより構成することができ、複数種類のフィルムが積層されたものであってもよい。蓋体１２００は、液体収容部１１３０の放出口１１３１の側の端部に接着剤や、熱や超音波による溶着等の手段により固着されることができる。 The lid 1200 that seals the discharge port 1131 in the liquid storage portion 1130 of the container body 1100 is configured to be easily broken. The lid 1200 can be made of a film containing a synthetic resin or a metal film such as an aluminum film, and a plurality of types of films may be laminated. The lid 1200 can be fixed to the end of the liquid storage portion 1130 on the side of the discharge port 1131 by means such as adhesive or welding by heat or ultrasonic waves.

本実施形態の液体収容容器１０００は、逆止部１１４０を備えていることを特徴とする。逆止部１１４０は、第２空間１１０２から第１空間１１０１への液体ＬＡの流入を妨げ、且つ、蛇腹部１１２０を圧縮変形させたときの第１空間１１０１から第２空間１１０２への気体の流入を許容する。The liquid storage container 1000 of the present embodiment is characterized by including a check valve 1140. Check unit 1140, the second space 1102 impede the flow of the liquid L A of the first space 1101, and, of the gas from the first space 1101 when is compressed deforming the bellows portion 1120 to the second space 1102 Allow inflow.

本明細書において「第２空間から第１空間への液体の流入を妨げる」とは、第２空間から第１空間への液体の流入を完全に阻害することだけでなく、装置の使用目的において許容される範囲で第２空間から第１空間への液体の流入を実質的に阻害することも包含する。 In the present specification, "preventing the inflow of liquid from the second space to the first space" means not only completely inhibiting the inflow of liquid from the second space to the first space, but also for the purpose of use of the device. It also includes substantially inhibiting the inflow of liquid from the second space to the first space to the extent permitted.

図７Ｃに示すように、本実施形態の液体収容容器１０００において、蛇腹部１１２０を軸Ｘに沿って圧縮変形させるとき、液体収容容器１０００の第１空間１１０１を囲う蛇腹部１１２０の内壁面１１２０Ｂで個々の谷Ｖを構成する一対の壁面Ｖ１，Ｖ２間の軸Ｘの方向の距離が縮まる。一対の壁面Ｖ１，Ｖ２同士が密着する場合もある。すなわち、蛇腹部１１２０の圧縮変形により蛇腹部１１２０の内壁面１１２０Ｂの複数の谷Ｖの幅は、圧縮変形前と比較して小さくなる。As shown in FIG. 7C, in the liquid storage container 1000 of the present embodiment, when the bellows portion 1120 is compressed and deformed along the axis X, the inner wall surface 1120B of the bellows portion 1120 surrounding the first space 1101 of the liquid storage container 1000 The distance in the direction of the axis X between the pair of wall surfaces V 1 and V 2 constituting the individual valleys V is shortened. The pair of wall surfaces V 1 and V 2 may be in close contact with each other. That is, due to the compression deformation of the bellows portion 1120, the width of the plurality of valleys V of the inner wall surface 1120B of the bellows portion 1120 becomes smaller than that before the compression deformation.

本実施形態の液体収容容器１０００を用い、放出口１１３１を開放し、蛇腹部１１２０を圧縮変形する際に、仮に逆止部１１４０が存在せず、蛇腹部１１２０の内壁面１１２０Ｂに接触している液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物が存在している場合には、該液体ＬＡ又は該混合物は、幅の小さくなった複数の谷Ｖに毛管現象により入り込み保持されるため、放出されずに液体収容容器１０００内に残留するという問題がある。When the discharge port 1131 is opened and the bellows portion 1120 is compressed and deformed by using the liquid storage container 1000 of the present embodiment, the check portion 1140 does not exist and is in contact with the inner wall surface 1120B of the bellows portion 1120. when the mixture of liquid L a or liquid L a and other components (analytes, etc.) are present, the liquid L a or the mixture enters by capillarity into a plurality of valleys V becomes smaller in width Since it is retained, there is a problem that it remains in the liquid storage container 1000 without being released.

そこで本実施形態の液体収容容器１０００では、第１空間１１０１と第２空間１１０２との間を区切るように逆止部１１４０を設けることで、本実施形態の液体収容容器１０００の保存、輸送、又は、使用の各時点において、第２空間１１０２に収容された液体ＬＡ又は液体ＬＡと他の成分との混合物が、第１空間１１０１に流入することを妨げ、上記の問題を解消する。Therefore, in the liquid storage container 1000 of the present embodiment, by providing a check portion 1140 so as to separate the first space 1101 and the second space 1102, storage, transportation, or storage or transportation of the liquid storage container 1000 of the present embodiment is performed. , at each point of use, the mixture of liquid L a or liquid L a and other components housed in the second space 1102, prevented from flowing into the first space 1101, to solve the above problems.

本発明において逆止部の具体的な構造は特に限定されない。逆止部の具体的な実施形態の１つが、図３Ｂ等に図示する逆止部１１４０である。 In the present invention, the specific structure of the check valve is not particularly limited. One of the specific embodiments of the check valve is the check valve 1140 shown in FIG. 3B and the like.

逆止部１１４０の構造について図１３Ａ（斜視図）及び図１３Ｂ（断面図）を参照して説明する。 The structure of the check valve 1140 will be described with reference to FIGS. 13A (perspective view) and 13B (cross-sectional view).

逆止部１１４０は、第１空間１１０１と第２空間１１０２とを接続する貫通孔１１４１を内側に有する管部１１４２と、管部１１４２の第２空間１１０２側の端１１４２Ａから径方向外方に広がる板状部１１４３と、板状部１１４３の外縁部１１４３Ａに接続し、第１空間１１０１の側及び第２空間１１０２の側に延びた筒状の側壁部１１４４と、側壁部１１４４の第２空間１１０２の側の端１１４４Ｃから径方向外方に広がる位置決め部１１４５とを備える。逆止部１１４０の側壁部１１４４の外周面１１４４Ａ上には、全周を囲うように周方向に延びる、径方向外方に突出した、弾性変形可能なリング状突起１１４４Ｂが設けられている。逆止部１１４０は、図３Ｂに示すように、第２空間１１０２の側から、容器本体１１００の接続部１１８０に嵌入され、リング状突起１１４４Ｂが接続部１１８０の内周面１１８０Ａと当接して弾性変形することで、接続部１１８０内に固定される。逆止部１１４０の位置決め部１１４５は、容器本体１１００の段部１１８１と当接して、逆止部１１４０の第１空間１１０１側への脱落を防止する。 The check portion 1140 extends radially outward from the pipe portion 1142 having a through hole 1141 connecting the first space 1101 and the second space 1102 inside and the end 1142A of the pipe portion 1142 on the second space 1102 side. A tubular side wall portion 1144 connected to the plate-shaped portion 1143 and the outer edge portion 1143A of the plate-shaped portion 1143 and extending to the side of the first space 1101 and the side of the second space 1102, and the second space 1102 of the side wall portion 1144. It is provided with a positioning portion 1145 extending radially outward from the end 1144C on the side of. On the outer peripheral surface 1144A of the side wall portion 1144 of the non-return portion 1140, an elastically deformable ring-shaped protrusion 1144B extending in the circumferential direction so as to surround the entire circumference is provided. As shown in FIG. 3B, the non-stop portion 1140 is fitted into the connecting portion 1180 of the container body 1100 from the side of the second space 1102, and the ring-shaped protrusion 1144B abuts on the inner peripheral surface 1180A of the connecting portion 1180 and is elastic. By deforming, it is fixed in the connection portion 1180. The positioning portion 1145 of the check portion 1140 comes into contact with the step portion 1181 of the container body 1100 to prevent the check portion 1140 from falling off to the first space 1101 side.

逆止部１１４０を備える液体収容容器１０００では、第１空間１１０１と第２空間１１０２とは貫通孔１１４１のみにより接続される。この実施形態によれば、液体収容容器１０００の輸送、保存、使用の各段階において、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物が、第２空間１１０２の側から第１空間１１０１の側に流入することが実質的に阻止されるため、上記の問題を解決することができる。また、放出口１１３１を開放し、蛇腹部１１２０を圧縮変形する際には、第１空間１１０１内で圧縮された気体は貫通孔１１４１を通り第２空間１１０２に流入することができるため、逆止部１１４０は、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物を放出口１１３１から圧送する際に支障にはならない。In the liquid storage container 1000 provided with the check valve 1140, the first space 1101 and the second space 1102 are connected only by the through hole 1141. According to this embodiment, the transport of the liquid container 1000, save, at each stage of use, the mixture of liquid L A or liquid L A and the other component (analyte or the like), first from the side of the second space 1102 Since the inflow to the side of one space 1101 is substantially prevented, the above problem can be solved. Further, when the discharge port 1131 is opened and the bellows portion 1120 is compressed and deformed, the gas compressed in the first space 1101 can flow into the second space 1102 through the through hole 1141, so that the check is stopped. part 1140, not a hindrance when pumping a mixture of liquid L a or liquid L a and the other component (analyte or the like) from the outlet 1131.

逆止部１１４０において、貫通孔１１４１の寸法は特に限定されないが、第２空間１１０２の側から第１空間１１０１の側に流入をより効果的に阻害するためには、貫通孔１１４１の最狭部の幅Ｗ（図示する例では軸Ｘに垂直な方向の幅）は０．５ｍｍ以下であることが好ましく、長さＨ（図示する例では軸Ｘ方向の長さ）は０．１ｍｍ以上であることが好ましい。 In the non-stop portion 1140, the size of the through hole 1141 is not particularly limited, but in order to more effectively block the inflow from the side of the second space 1102 to the side of the first space 1101, the narrowest portion of the through hole 1141 The width W (width in the direction perpendicular to the axis X in the illustrated example) is preferably 0.5 mm or less, and the length H (length in the axis X direction in the illustrated example) is 0.1 mm or more. Is preferable.

図示する逆止部１１４０では貫通孔１１４１は一つのみ形成されているが、複数の貫通孔を有する逆止部であってもよい。 Although only one through hole 1141 is formed in the non-stop portion 1140 shown in the figure, it may be a non-stop portion having a plurality of through holes.

本実施形態の液体収容容器１０００は、ロック部２０と組み合わせて、図３Ａ及び３Ｂに示すユニット２を構成することができる。ユニット２は、後述する核酸分離装置１に用いることができる。 The liquid storage container 1000 of the present embodiment can form the unit 2 shown in FIGS. 3A and 3B in combination with the lock portion 20. The unit 2 can be used in the nucleic acid separation device 1 described later.

図１はロック部２０の外観を示す。ロック部２０は、蛇腹部１１２０の周囲に、軸Ｘの方向に沿って延び、蛇腹部１１２０の外表面１１２０Ａの一部分を覆うように配置された筒状の案内部２１０と、筒状の案内部２１０の、蛇腹部１１２０の押圧部１１２１の側に位置する端部２１０Ａに接続され、蛇腹部１１２０の押圧部１１２１と連結される連結部２２０と、筒状の案内部２１０の他方の端部２１０Ｂの近傍の、筒状の案内部２１０の内周面２１０Ｃ上に、内方に突出するように配置されたロック部側係止部（第２係止部）２３０とを備える。ロック部側係止部（第２係止部）２３０は、周方向に延在する係止爪であり、本実施形態では周方向の異なる位置に３つ設けられている。 FIG. 1 shows the appearance of the lock portion 20. The lock portion 20 has a tubular guide portion 210 arranged around the bellows portion 1120 so as to extend along the direction of the axis X and cover a part of the outer surface 1120A of the bellows portion 1120, and a tubular guide portion. The connecting portion 220 of 210, which is connected to the end 210A located on the side of the pressing portion 1121 of the bellows portion 1120 and connected to the pressing portion 1121 of the bellows portion 1120, and the other end 210B of the tubular guide portion 210. A locking portion-side locking portion (second locking portion) 230 arranged so as to project inward is provided on the inner peripheral surface 210C of the tubular guide portion 210 in the vicinity of the above. The locking portion (second locking portion) 230 on the locking portion side is a locking claw extending in the circumferential direction, and in the present embodiment, three are provided at different positions in the circumferential direction.

図３Ａ及び３Ｂに示すように、液体収容容器１０００とロック部２０とは、液体収容容器１０００の容器本体１１００の押圧部１１２１に、ロック部２０の連結部２２０が固定されて一体化され、ユニット２を構成する。ユニット２は、押圧部１１２１を介した液体収容容器１０００の蛇腹部１１２０への意図しない押圧を制限するために、取り外し可能なストッパー部材３０を更に備えることが好ましい。ストッパー部材３０は、容器本体１１００の接続部１１８０の周囲に取り外し可能に固定され、一端が、ロック部２０の案内部２１０の端部２１０Ｂに当接し、他端が、段部１１８１に当接する。ストッパー部材３０を備えるユニット２は、後述する、担体保持部１２０へ接続する操作が容易となるため好ましい。 As shown in FIGS. 3A and 3B, the liquid storage container 1000 and the lock portion 20 are integrated by fixing the connecting portion 220 of the lock portion 20 to the pressing portion 1121 of the container body 1100 of the liquid storage container 1000. 2 is configured. It is preferable that the unit 2 further includes a removable stopper member 30 in order to limit unintended pressing of the liquid storage container 1000 on the bellows portion 1120 via the pressing portion 1121. The stopper member 30 is removably fixed around the connecting portion 1180 of the container body 1100, one end of which abuts on the end 210B of the guide portion 210 of the lock portion 20, and the other end of which abuts on the step portion 1181. The unit 2 provided with the stopper member 30 is preferable because it facilitates the operation of connecting to the carrier holding portion 120, which will be described later.

貫通孔１１４１を有する逆止部１１４０の他の実施形態としては、図１４に示すように、貫通孔１１４１を閉塞し、蛇腹部１１２０を圧縮変形させたときに貫通孔１１４１を開放する閉塞部１１４７を更に備える実施形態が例示できる。この実施形態において、閉塞部１１４７は、貫通孔１１４１の第２空間１１０２側の部分の内寸とほぼ同一の外寸を有し、蛇腹部１１２０の圧縮変形により生じる第１空間１１０１と第２空間１１０２との気圧差により容易に脱落可能な強度で貫通孔１１４１の前記部分に挿入され固定された挿入部１１４７Ａを有する。この実施形態によれば、蛇腹部１１２０を圧縮変形させるまでは、逆止部１１４０の貫通孔１１４１は閉塞部１１４７により閉鎖されるため、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物が、第２空間１１０２の側から第１空間１１０１の側に流入することをより確実に阻止することができる。しかも、放出口１１３１を開放し、蛇腹部１１２０を圧縮変形する際には、発生した圧力差により閉塞部１１４７は第２空間１１０２の側に押し出されて脱落するため、第１空間１１０１内で圧縮された気体は貫通孔１１４１を通り第２空間１１０２に流入することができる。このため閉塞部１１４７を有する逆止部１１４０は、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物を放出口１１３１から圧送する際に支障にはならない。As another embodiment of the check valve 1140 having the through hole 1141, as shown in FIG. 14, the closing portion 1147 that closes the through hole 1141 and opens the through hole 1141 when the bellows portion 1120 is compressively deformed. An embodiment further comprising the above can be exemplified. In this embodiment, the closed portion 1147 has an outer dimension substantially the same as the inner dimension of the portion of the through hole 1141 on the second space 1102 side, and the first space 1101 and the second space generated by the compressive deformation of the bellows portion 1120. It has an insertion portion 1147A that is inserted and fixed in the portion of the through hole 1141 with a strength that allows it to easily fall off due to a pressure difference from 1102. According to this embodiment, until to compressive deformation of the bellows portion 1120, since the through-hole 1141 of the check unit 1140 is closed by the closing portion 1147, the liquid L A or liquid L A and other components (analytes, etc.) It is possible to more reliably prevent the mixture with and from flowing from the side of the second space 1102 to the side of the first space 1101. Moreover, when the discharge port 1131 is opened and the bellows portion 1120 is compressed and deformed, the closed portion 1147 is pushed out to the side of the second space 1102 due to the generated pressure difference and falls off, so that the closed portion 1147 is compressed in the first space 1101. The gas can flow into the second space 1102 through the through hole 1141. The check unit 1140 having an occlusion 1147 because, not a hindrance when pumping a mixture of liquid L A or liquid L A and the other component (analyte or the like) from the outlet 1131.

逆止部の他の実施形態として、図１５に示す逆止部１１５０、図１６に示す逆止部１１６０が例示できる。 As another embodiment of the check valve, the check valve 1150 shown in FIG. 15 and the check valve 1160 shown in FIG. 16 can be exemplified.

図１５には、逆止部１１５０を備えた液体収容容器１０００と、後述するロック部２０とを組み合わせたユニット２を示す。 FIG. 15 shows a unit 2 in which a liquid storage container 1000 provided with a check valve 1150 and a lock portion 20 described later are combined.

逆止部１１５０は、第１空間１１０１と第２空間１１０２とを仕切るように配置された多孔質体１１５１を備える。逆止部１１５０は更に、多孔質体１１５１を支持するための構造として、筒状の側壁部１１５２と、側壁部１１５２の内周面から径方向内方に延びる内フランジ部１１５３と、側壁部１１５２の第２空間１１０２の側の端１１５２Ｃから径方向外方に広がる位置決め部１１５４とを備える。逆止部１１５０が備える側壁部１１５２及び位置決め部１１５４の具体的な構造及び機能は、逆止部１１４０が備える側壁部１１４４及び位置決め部１１４５と同様である。 The check valve 1150 includes a porous body 1151 arranged so as to partition the first space 1101 and the second space 1102. The check portion 1150 further has a tubular side wall portion 1152, an inner flange portion 1153 extending radially inward from the inner peripheral surface of the side wall portion 1152, and a side wall portion 1152 as a structure for supporting the porous body 1151. It is provided with a positioning portion 1154 extending outward in the radial direction from the end 1152C on the side of the second space 1102. The specific structure and function of the side wall portion 1152 and the positioning portion 1154 included in the non-return portion 1150 are the same as those of the side wall portion 1144 and the positioning portion 1145 included in the non-return portion 1140.

逆止部１１５０の多孔質体１１５１は、微細な細孔を有し、気体が通過することができるものであれば良く、不織布、織布、紙、樹脂発泡体等が例示できる。 The porous body 1151 of the check portion 1150 may have fine pores and allows gas to pass through, and examples thereof include non-woven fabrics, woven fabrics, papers, and resin foams.

多孔質体１１５１を備える逆止部１１５０によれば、液体収容容器１０００の輸送、保存、使用の各段階において、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物が、第２空間１１０２の側から第１空間１１０１の側に流入することが実質的に阻止される。また、放出口１１３１を開放し、蛇腹部１１２０を圧縮変形する際には、第１空間１１０１内で圧縮された気体は多孔質体１１５１を通り第２空間１１０２に流入することができるため、逆止部１１５０は、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物を放出口１１３１から圧送する際に支障にはならない。According to the check unit 1150 provided with a porous material 1151, transport of the liquid container 1000, save, at each stage of use, the mixture of liquid L A or liquid L A and other components (analytes and the like), the The inflow from the side of the two spaces 1102 to the side of the first space 1101 is substantially prevented. Further, when the discharge port 1131 is opened and the bellows portion 1120 is compressed and deformed, the gas compressed in the first space 1101 can flow into the second space 1102 through the porous body 1151, so that the reverse is true. stop portion 1150, not a hindrance when pumping a mixture of liquid L a or liquid L a and the other component (analyte or the like) from the outlet 1131.

図１６には、逆止部１１６０を備えた液体収容容器１０００と、後述するロック部２０とを組み合わせたユニット２を示す。 FIG. 16 shows a unit 2 in which a liquid storage container 1000 provided with a check valve 1160 and a lock portion 20 described later are combined.

逆止部１１６０は、逆止弁の一例である。逆止弁である逆止部１１６０は、弁箱第１部材１１６３と弁箱第２部材１１６４とが組み合わされた弁箱と、弁箱中に配置された弁体１１６１と、スプリング１１６２とを備える。弁箱第１部材１１６３には第１開口１１６３Ａが形成されており、弁箱第２部材１１６４には第２開口１１６４Ａが形成されている。弁体１１６１は、弁箱第１部材１１６３の第１開口１１６３Ａを弁箱内側から塞ぐように配置される。スプリング１１６２は、弁体１１６１を弁箱第１部材１１６３に向けて付勢する。逆止部１１６０は、図１６に示すように、第２空間１１０２の側から、容器本体１１００の接続部１１８０に嵌入され、弁箱第１部材１１６３の外周面上のリング状突起１１６３Ｂが接続部１１８０の内周面１１８０Ａと当接して弾性変形することで、接続部１１８０内に固定される。 The check valve 1160 is an example of a check valve. The check valve 1160 includes a valve box in which the valve box first member 1163 and the valve box second member 1164 are combined, a valve body 1161 arranged in the valve box, and a spring 1162. .. A first opening 1163A is formed in the valve box first member 1163, and a second opening 1164A is formed in the valve box second member 1164. The valve body 1161 is arranged so as to close the first opening 1163A of the valve box first member 1163 from the inside of the valve box. The spring 1162 urges the valve body 1161 toward the valve box first member 1163. As shown in FIG. 16, the check portion 1160 is fitted into the connection portion 1180 of the container body 1100 from the side of the second space 1102, and the ring-shaped protrusion 1163B on the outer peripheral surface of the valve box first member 1163 is the connection portion. It is fixed in the connecting portion 1180 by abutting with the inner peripheral surface 1180A of the 1180 and elastically deforming.

逆止弁である逆止部１１６０によれば、液体収容容器１０００の輸送、保存、使用の各段階において、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物が、第２空間１１０２の側から第１空間１１０１の側に流入することが、逆止弁以外の実施形態の逆止部１１４０、１１５０と比較してより確実に阻止される。また、放出口１１３１を開放し、蛇腹部１１２０を圧縮変形する際には、第１空間１１０１内で圧縮された気体は、弁体１１６１を第２空間１１０２側に押し込み第２空間１１０２に流入することができるため、逆止部１１６０は、液体ＬＡ又は液体ＬＡと他の成分（検体等）との混合物を放出口１１３１から圧送する際に支障にはならない。According to the check unit 1160 is a check valve, the transport of the liquid container 1000, save, at each stage of use, the mixture of liquid L A or liquid L A and other components (analytes and the like), the second The inflow from the side of the space 1102 to the side of the first space 1101 is more reliably prevented as compared with the check portions 1140 and 1150 of the embodiments other than the check valve. Further, when the discharge port 1131 is opened and the bellows portion 1120 is compressed and deformed, the gas compressed in the first space 1101 pushes the valve body 1161 toward the second space 1102 and flows into the second space 1102. it is possible, check unit 1160, not a hindrance when pumping a mixture of liquid L a or liquid L a and the other component (analyte or the like) from the outlet 1131.

本発明において逆止部として用いることができる逆止弁の構造としては、図１６に示す構造には限定されず、他の構造の逆止弁を用いてもよい。 The structure of the check valve that can be used as the check valve in the present invention is not limited to the structure shown in FIG. 16, and a check valve having another structure may be used.

＜核酸分離装置＞
上記実施形態の液体収容容器１０００を備える核酸分離装置の一実施形態として、図７Ａ〜７Ｄに図示する核酸分離装置１について説明する。<Nucleic acid separator>
As an embodiment of the nucleic acid separation device including the liquid storage container 1000 of the above embodiment, the nucleic acid separation device 1 illustrated in FIGS. 7A to 7D will be described.

液体収容容器１０００を核酸分離装置１に用いる場合、液体収容容器１０００の第１空間１１０１に収容される液体ＬＡは、典型的には、核酸を処理するための薬液、核酸を含有する検体を処理するための薬液、又は核酸を担持した担体を処理するための薬液である。核酸を含有する検体を処理するための薬液としては、核酸を検体中で遊離させる試薬を含む液体が例示できる。このような液体は、例えば、アルカリ物質と、チオール系還元剤と、アルコールとを含む水溶液である。アルカリ物質としては水酸化ナトリウム又は水酸化カリウムが好ましく、チオール系還元剤としてはＮ−アセチル−Ｌ−システインが好ましく、アルコールとしてはエタノールが好ましい。When using the liquid container 1000 to the nucleic acid separation device 1, the liquid L A accommodated in the first space 1101 of the liquid container 1000 is typically chemical for processing a nucleic acid, a specimen containing a nucleic acid A chemical solution for processing or a chemical solution for treating a carrier carrying a nucleic acid. Examples of the drug solution for processing a sample containing nucleic acid include a liquid containing a reagent that releases nucleic acid in the sample. Such a liquid is, for example, an aqueous solution containing an alkaline substance, a thiol-based reducing agent, and an alcohol. Sodium hydroxide or potassium hydroxide is preferable as the alkaline substance, N-acetyl-L-cysteine is preferable as the thiol-based reducing agent, and ethanol is preferable as the alcohol.

核酸分離装置１は、液体収容容器１０００と、液透過性担体１３（以下「担体１３」と表す場合がある）を保持する担体保持部１２０とが組み合わされて一体化した核酸処理容器１０と、ロック部２０とを備える。 The nucleic acid separation device 1 includes a nucleic acid processing container 10 in which a liquid storage container 1000 and a carrier holding portion 120 holding a liquid permeable carrier 13 (hereinafter, may be referred to as “carrier 13”) are combined and integrated. A lock portion 20 is provided.

担体保持部１２０は、担体１３と、それを保持する担体保持部本体１３０とを備える。 The carrier holding portion 120 includes a carrier 13 and a carrier holding portion main body 130 that holds the carrier 13.

まず、担体１３と担体保持部本体１３０とを備える担体保持部１２０について説明する。 First, the carrier holding portion 120 including the carrier 13 and the carrier holding portion main body 130 will be described.

担体保持部本体１３０は、検体を採取するための第１部材１３１０と、担体１３を保持する第２部材１３２０とが組み合わされて一体化されたものである。 The carrier holding unit main body 130 is a combination of a first member 1310 for collecting a sample and a second member 1320 for holding the carrier 13.

第１部材１３１０の正面図を図４Ａに、上面図を図４Ｂに、図４ＢでのＡ−Ａ線による断面図を図４Ｃにそれぞれ示す。 The front view of the first member 1310 is shown in FIG. 4A, the top view is shown in FIG. 4B, and the cross-sectional view taken along the line AA in FIG. 4B is shown in FIG. 4C.

本実施形態では、第１部材１３１０は、薬液供給口１３１１が一端に形成された筒状の薬液供給口周縁部１３１２と、薬液供給口周縁部１３１２の、薬液供給口１３１１の側の端部１３１２Ａとは反対側の端部である下流側端部１３１２Ｂに接続され、薬液供給口１３１１とは反対方向に延在する、筒状の第１延在部１３１３とを備える。薬液供給口周縁部１３１２は更に、外周面１３１２Ｃ上に、周方向の全体に亘って延在し、径方向外方に突出した係止爪である、液体収容部側係止部（第１係止部）１３１２Ｄを備える。第１延在部１３１３の内周面１３１３Ａには、後述する第２部材１３２０の第３延在部１３２２の第３螺合部１３２２Ｂと螺合するための第１螺合部１３１３Ｂが設けられている。第１部材１３１０は更に、薬液供給口周縁部１３１２の下流側端部１３１２Ｂから、径方向内方に張り出した内フランジ部１３１４と、内フランジ部１３１４の内側端１３１４Ａから、薬液供給口１３１１とは反対方向に延在する筒状の第２延在部１３１５とを備える。第１部材１３１０は更に、第２延在部１３１５の内周面１３１５Ａの対向する部分を、第２延在部１３１５の延在方向と直交する方向に延在して架橋する、第２延在部１３１５の延在方向に沿って見た時の形状が略十字状となる架橋部１３１６と、架橋部１３１６から薬液供給口１３１１に向け立設された第１突出部１３１７とを備える。第１突出部１３１７の先端には、薬液供給口１３１１に向けて先鋭となった複数の突起１３１７Ａが設けられている。更に、架橋部１３１６と、第２延在部１３１５の内周面１３１５Ａとの間には、第２延在部１３１５の延在方向と直交する方向に沿って、液体が自由に通過できるフィルタ１３１８が配置されている。フィルタ１３１８は、比較的寸法の大きい粗大な固形物を取り除く目的で配置されており、具体的な構造としては、メッシュ（網）、繊維の集合体（織布、不織布、ろ紙等）、金属又は樹脂を焼結した焼結ろ材等が例示できる。第１部材１３１０は更に、内フランジ部１３１４の、薬液供給口１３１１の側の面１３１４Ｂ上に、薬液供給口１３１１に向け立設された、周方向に等間隔に配置された１２本の第２突出部１３１９を備える。第２突出部１３１９の先端には、薬液供給口１３１１に向けて先鋭となった先鋭部１３１９Ａが設けられている。 In the present embodiment, the first member 1310 has a tubular chemical solution supply port peripheral edge portion 1312 in which a chemical solution supply port 1311 is formed at one end, and an end portion 1312A of the chemical solution supply port peripheral edge portion 1312 on the side of the chemical solution supply port 1311. It is provided with a tubular first extending portion 1313 that is connected to the downstream end portion 1312B, which is the end portion on the opposite side to the above, and extends in the direction opposite to the chemical solution supply port 1311. The chemical liquid supply port peripheral edge portion 1312 is a locking claw that extends over the entire circumferential direction and protrudes outward in the radial direction on the outer peripheral surface 1312C. Stop) 1312D is provided. The inner peripheral surface 1313A of the first extending portion 1313 is provided with a first screwing portion 1313B for screwing with the third screwing portion 1322B of the third extending portion 1322 of the second member 1320, which will be described later. There is. Further, the first member 1310 has an inner flange portion 1314 protruding inward in the radial direction from the downstream end portion 1312B of the chemical liquid supply port peripheral edge portion 1312, and the chemical liquid supply port 1311 from the inner end 1314A of the inner flange portion 1314. It is provided with a tubular second extending portion 1315 extending in the opposite direction. The first member 1310 further extends and bridges the facing portion of the inner peripheral surface 1315A of the second extending portion 1315 in a direction orthogonal to the extending direction of the second extending portion 1315. It includes a cross-linked portion 1316 having a substantially cross-shaped shape when viewed along the extending direction of the portion 1315, and a first protruding portion 1317 erected from the cross-linked portion 1316 toward the chemical solution supply port 1311. At the tip of the first protrusion 1317, a plurality of protrusions 1317A that are sharpened toward the chemical supply port 1311 are provided. Further, a filter 1318 through which a liquid can freely pass between the crosslinked portion 1316 and the inner peripheral surface 1315A of the second extending portion 1315 along a direction orthogonal to the extending direction of the second extending portion 1315. Is placed. The filter 1318 is arranged for the purpose of removing coarse solid matter having a relatively large size, and as a specific structure, a mesh (net), an aggregate of fibers (woven fabric, non-woven fabric, filter paper, etc.), metal or An example is a sintered filter paper obtained by sintering a resin. The first member 1310 is further provided with twelve second members arranged at equal intervals in the circumferential direction, which are erected toward the chemical solution supply port 1311 on the surface 1314B of the inner flange portion 1314 on the side of the chemical solution supply port 1311. A protrusion 1319 is provided. At the tip of the second protruding portion 1319, a sharpened portion 1319A that is sharpened toward the chemical solution supply port 1311 is provided.

担体１３と組み合わせた第２部材１３２０の正面図を図５Ａに、上面図を図５Ｂに、図５ＢでのＡ−Ａ線による断面図を図５Ｃにそれぞれ示す。 A front view of the second member 1320 combined with the carrier 13 is shown in FIG. 5A, a top view is shown in FIG. 5B, and a cross-sectional view taken along the line AA in FIG. 5B is shown in FIG. 5C.

本実施形態では、第２部材１３２０は、一端に開口１３２１が形成された筒状の第３延在部１３２２を備える。第３延在部１３２２の外径は、第１部材１３１０の第１延在部１３１３の内径よりも小さい。第３延在部１３２２の外周面１３２２Ａには、第１部材１３１０の第１延在部１３１３の第１螺合部１３１３Ｂと螺合するための第３螺合部１３２２Ｂが設けられている。第３延在部１３２２の開口１３２１付近の内径は、第１部材１３１０の第２延在部１３１５の外径よりも大きい。第３延在部１３２２のうち、開口１３２１が形成された端部と反対側の端部である下流側端部１３２２Ｃ寄りの部分には、内径が小さくなるように径方向内方に膨出した膨出部１３２２Ｄが形成されており、第３延在部１３２２の膨出部１３２２Ｄでの内径は、第１部材１３１０の第２延在部１３１５の外径よりも僅かに小さい。第２部材１３２０は更に、第３延在部１３２２の下流側端部１３２２Ｃに接続された排出部１３２３を備える。排出部１３２３は、径方向内方に近づくほど、連続的又は段階的に開口１３２１から遠ざかる内側面１３２３Ａを有し、且つ、末端に排出口１２が形成されている。第２部材１３２０は更に、排出部１３２３の内側面１３２３Ａ上に、開口１３２１に向け立設された、周方向に等間隔に配置された４つの第４突出部１３２６を備える。第２部材１３２０の排出部１３２３の、排出口１２から排出される液体の流路となる位置に、担体１３が配置される。第２部材１３２０は更に、第３延在部１３２２の下流側端部１３２２Ｃに接続され、径方向外方に向け張り出したフランジ部１３２４と、フランジ部１３２４の外周縁部１３２４Ａに接続され、開口１３２１とは反対方向に延在する、筒状の第４延在部１３２５を備える。第４延在部１３２５の内周面１３２５Ａには、後述する回収容器４０の首部４２の外周面４２Ａに設けられた第５螺合部４２Ｂと螺合するための第４螺合部１３２５Ｂが設けられている。 In the present embodiment, the second member 1320 includes a tubular third extending portion 1322 having an opening 1321 formed at one end. The outer diameter of the third extending portion 1322 is smaller than the inner diameter of the first extending portion 1313 of the first member 1310. The outer peripheral surface 1322A of the third extending portion 1322 is provided with a third screwing portion 1322B for screwing with the first screwing portion 1313B of the first extending portion 1313 of the first member 1310. The inner diameter of the third extending portion 1322 near the opening 1321 is larger than the outer diameter of the second extending portion 1315 of the first member 1310. Of the third extending portion 1322, the portion closer to the downstream end portion 1322C, which is the end opposite to the end on which the opening 1321 is formed, bulges inward in the radial direction so that the inner diameter becomes smaller. The bulging portion 1322D is formed, and the inner diameter of the third extending portion 1322 at the bulging portion 1322D is slightly smaller than the outer diameter of the second extending portion 1315 of the first member 1310. The second member 1320 further comprises a discharge portion 1323 connected to a downstream end portion 1322C of the third extending portion 1322. The discharge portion 1323 has an inner side surface 1323A that continuously or stepwise moves away from the opening 1321 as it approaches inward in the radial direction, and a discharge port 12 is formed at the end thereof. The second member 1320 further includes four fourth protruding portions 1326 arranged at equal intervals in the circumferential direction, which are erected toward the opening 1321 on the inner side surface 1323A of the discharging portion 1323. The carrier 13 is arranged at a position of the discharge portion 1323 of the second member 1320, which serves as a flow path for the liquid discharged from the discharge port 12. The second member 1320 is further connected to the downstream end portion 1322C of the third extending portion 1322, and is connected to the flange portion 1324 protruding outward in the radial direction and the outer peripheral edge portion 1324A of the flange portion 1324, and the opening 1321. It is provided with a tubular fourth extending portion 1325 extending in the opposite direction to the above. The inner peripheral surface 1325A of the fourth extending portion 1325 is provided with a fourth screwed portion 1325B for screwing with the fifth screwed portion 42B provided on the outer peripheral surface 42A of the neck portion 42 of the collection container 40, which will be described later. Has been done.

担体１３は、液透過性担体であれば特に限定されないが、好ましくは、核酸を吸着する又は核酸を通過させる液透過性担体である。液透過性担体としては、シリカ等の無機担体、樹脂、不溶性多糖等の有機担体が例示されるがこれに限定されない。これらの担体は粉末状、繊維状、多孔質状であっても良く、磁性を帯びていても良く、任意の修飾をされていても良い。液透過性担体はカラム状、メンブレン状等の形状に成形されたものを用いることができる。メンブレン状の液透過性担体の厚さは特に限定されないが、好ましくは１．０ｍｍ〜１．５ｍｍである。メンブレン状の液透過性担体の平面視形状は特に限定されないが、好ましくは直径１ｍｍ〜１０ｍｍの円形である。多孔質状の核酸吸着性担体としては、例えば、モノリス構造の多孔質担体が例示でき、特に好ましくはモノリス型シリカである。モノリス型シリカとしては、走査型電子顕微鏡（ＳＥＭ）写真から求めたスルーポア径の平均値が２０〜５０μｍであることが好ましく、厚さが１．０〜２．０ｍｍであることが好ましい。 The carrier 13 is not particularly limited as long as it is a liquid-permeable carrier, but is preferably a liquid-permeable carrier that adsorbs or passes nucleic acids. Examples of the liquid-permeable carrier include, but are not limited to, an inorganic carrier such as silica and an organic carrier such as a resin and an insoluble polysaccharide. These carriers may be powdery, fibrous, porous, magnetic, or optionally modified. As the liquid permeable carrier, one formed into a column shape, a membrane shape, or the like can be used. The thickness of the membrane-like liquid-permeable carrier is not particularly limited, but is preferably 1.0 mm to 1.5 mm. The plan-view shape of the membrane-like liquid-permeable carrier is not particularly limited, but is preferably a circle having a diameter of 1 mm to 10 mm. Examples of the porous nucleic acid-adsorbing carrier include a porous carrier having a monolith structure, and a monolith-type silica is particularly preferable. As the monolith type silica, the average value of the through-pore diameter determined from the scanning electron microscope (SEM) photograph is preferably 20 to 50 μm, and the thickness is preferably 1.0 to 2.0 mm.

図６Ａに、第１部材１３１０と、第２部材１３２０と、担体１３を組み合わせて構成された担体保持部１２０の断面模式図を示す。第１部材１３１０と、第２部材１３２０とは、第１部材１３１０の第１延在部１３１３と、第２部材１３２０の第３延在部１３２２とを螺合することにより一体化される。第１部材１３１０と第２部材１３２０とからなる担体保持部本体１３０は、第１部材１３１０の内部空間と第２部材１３２０の内部空間とが一体となった、核酸を含む検体を収容するための検体収容空間１３１を内包する。このとき、第１部材１３１０の第２延在部１３１５の外周面１３１５Ｂに、第２部材１３２０の第３延在部１３２２の膨出部１３２２Ｄが当接して、検体収容空間１３１からの液漏れを防ぐ。 FIG. 6A shows a schematic cross-sectional view of the carrier holding portion 120 formed by combining the first member 1310, the second member 1320, and the carrier 13. The first member 1310 and the second member 1320 are integrated by screwing the first extending portion 1313 of the first member 1310 and the third extending portion 1322 of the second member 1320. The carrier holding portion main body 130 composed of the first member 1310 and the second member 1320 is for accommodating a sample containing nucleic acid in which the internal space of the first member 1310 and the internal space of the second member 1320 are integrated. It contains a sample storage space 131. At this time, the bulging portion 1322D of the third extending portion 1322 of the second member 1320 comes into contact with the outer peripheral surface 1315B of the second extending portion 1315 of the first member 1310 to prevent liquid leakage from the sample storage space 131. prevent.

担体保持部１２０では、検体収容空間１３１の上流側に、薬液供給口１３１１が形成されており、検体収容空間１３１の下流側に、薬液ＬＡと検体Ｓとの混合物である液体Ｌを、担体１３を介して排出する排出口１２が形成されている。The carrier holding portion 120, on the upstream side of the sample accommodating space 131, the chemical liquid supply port 1311 is formed on the downstream side of the sample accommodating space 131, the liquid L is a mixture of chemical L A and the specimen S, the carrier A discharge port 12 for discharging through 13 is formed.

図６Ａでは更に、液体を回収する回収容器４０を示す。回収容器４０の入口４１は、首部４２により囲われている。首部４２の外周面４２Ａには第５螺合部４２Ｂが設けられている。図６Ａに示すように、第２部材１３２０の第４延在部１３２５と、回収容器４０の首部４２とが螺合されて一体化される。第２部材１３２０と回収容器４０との接続は気密ではない。 FIG. 6A further shows a collection container 40 for collecting the liquid. The inlet 41 of the collection container 40 is surrounded by the neck 42. A fifth screwed portion 42B is provided on the outer peripheral surface 42A of the neck portion 42. As shown in FIG. 6A, the fourth extending portion 1325 of the second member 1320 and the neck portion 42 of the collection container 40 are screwed and integrated. The connection between the second member 1320 and the collection container 40 is not airtight.

図６Ｂに示すように、担体保持部１２０の検体収容空間１３１に、核酸を含む検体Ｓを収容し、続いて、図７Ａ及び７Ｂに示すように、液体収容容器１０００を担体保持部１２０に接続して核酸処理容器１０を形成し、核酸処理容器１０中で検体Ｓと薬液ＬＡとを混合して液体Ｌを生成する。As shown in FIG. 6B, the sample S containing nucleic acid is stored in the sample storage space 131 of the carrier holding unit 120, and then the liquid storage container 1000 is connected to the carrier holding unit 120 as shown in FIGS. 7A and 7B. to form a nucleic acid processing vessel 10, by mixing the specimen S and the chemical L a in nucleic acid processing vessel 10 to form a liquid L.

担体保持部１２０と液体収容容器１０００とを接続して核酸処理容器１０を形成する手順について説明する。 The procedure for connecting the carrier holding portion 120 and the liquid storage container 1000 to form the nucleic acid processing container 10 will be described.

液体収容容器１０００が備える容器本体１１００の液体収容部１１３０の外周面１１３０Ａには、図３Ｂに示すように、全周を囲うように周方向に延びるリング状凹溝１１３０Ｂ及び径方向外方に突出したリング状突起１１３０Ｃが設けられており、更に、全周を囲うように周方向に延びる弾性変形可能なＯリング１１３０Ｄが配置されている。 As shown in FIG. 3B, the outer peripheral surface 1130A of the liquid storage portion 1130 of the container body 1100 included in the liquid storage container 1000 has a ring-shaped concave groove 1130B extending in the circumferential direction so as to surround the entire circumference and protruding outward in the radial direction. A ring-shaped protrusion 1130C is provided, and an elastically deformable O-ring 1130D extending in the circumferential direction is arranged so as to surround the entire circumference.

一方、担体保持部１２０の薬液供給口周縁部１３１２の内周面１３１２Ｅには、全周を囲うように周方向に延び、径方向内方に突出した、２つのリング状突起１３１２Ｆが設けられている。 On the other hand, the inner peripheral surface 1312E of the peripheral edge portion 1312 of the chemical solution supply port of the carrier holding portion 120 is provided with two ring-shaped protrusions 1312F extending in the circumferential direction so as to surround the entire circumference and projecting inward in the radial direction. There is.

図７Ｂに示すように、液体収容容器１０００の液体収容部１１３０と、担体保持部１２０の薬液供給口周縁部１３１２とを嵌入して接続することで、液体収容容器１０００の放出口１１３１と、担体保持部１２０の薬液供給口１３１１とを接続することができる。このとき、Ｏリング１１３０Ｄは、容器本体１１００の液体収容部１１３０の外周面１１３０Ａと、担体保持部１２０の薬液供給口周縁部１３１２の内周面１３１２Ｅとの両方に当接して弾性変形し、それらの間の液体の流通を阻止する。また、容器本体１１００の液体収容部１１３０の外周面１１３０Ａ上のリング状凹溝１１３０Ｂ及びリング状突起１１３０Ｃと、担体保持部１２０の薬液供給口周縁部１３１２の内周面１３１２Ｅ上の２つのリング状突起１３１２Ｆとが互いに係合することで、容器本体１１００の液体収容部１１３０と担体保持部１２０の薬液供給口周縁部１３１２との接続を強固にする。 As shown in FIG. 7B, the liquid storage portion 1130 of the liquid storage container 1000 and the peripheral portion 1312 of the chemical solution supply port of the carrier holding unit 120 are fitted and connected to form the discharge port 1131 of the liquid storage container 1000 and the carrier. The chemical solution supply port 1311 of the holding unit 120 can be connected. At this time, the O-ring 1130D abuts on both the outer peripheral surface 1130A of the liquid storage portion 1130 of the container body 1100 and the inner peripheral surface 1312E of the chemical liquid supply port peripheral edge portion 1312 of the carrier holding portion 120, and is elastically deformed. Block the flow of liquid between. Further, two ring-shaped grooves 1130B and ring-shaped protrusions 1130B on the outer peripheral surface 1130A of the liquid storage portion 1130 of the container body 1100 and two ring-shaped portions 1312E on the inner peripheral surface 1312E of the peripheral edge portion 1312 of the chemical solution supply port of the carrier holding portion 120 By engaging the protrusions 1312F with each other, the connection between the liquid storage portion 1130 of the container body 1100 and the chemical liquid supply port peripheral edge portion 1312 of the carrier holding portion 120 is strengthened.

更に、容器本体１１００の液体収容部１１３０と担体保持部１２０の薬液供給口周縁部１３１２とを接続するとき、担体保持部１２０が備える、第１突出部１３１７の先端の複数の突起１３１７Ａと、第２突出部１３１９の先端の先鋭部１３１９Ａとが、液体収容容器１０００の放出口１１３１を封止する蓋体１２００に押し当てられて蓋体１２００を破壊し、液体収容容器１０００の放出口１１３１を開放する。すなわち、担体保持部１２０が備える、第１突出部１３１７及び第２突出部１３１９が開放部に相当する。この結果、液体収容容器１０００の第２空間１１０２と担体保持部１２０の検体収容空間１３１とが連通して一体となった収容空間１１を内包する核酸処理容器１０が形成される。 Further, when connecting the liquid storage portion 1130 of the container body 1100 and the peripheral edge portion 1312 of the chemical liquid supply port of the carrier holding portion 120, a plurality of protrusions 1317A at the tip of the first protruding portion 1317 included in the carrier holding portion 120, and a number of protrusions 1317A. 2 The sharpened portion 1319A at the tip of the protruding portion 1319 is pressed against the lid 1200 that seals the discharge port 1131 of the liquid storage container 1000 to destroy the lid 1200 and open the discharge port 1131 of the liquid storage container 1000. To do. That is, the first protruding portion 1317 and the second protruding portion 1319 included in the carrier holding portion 120 correspond to the open portions. As a result, the nucleic acid processing container 10 containing the storage space 11 in which the second space 1102 of the liquid storage container 1000 and the sample storage space 131 of the carrier holding portion 120 communicate with each other is formed.

核酸処理容器１０の収容空間１１中で検体Ｓと薬液ＬＡとを混合して液体Ｌが形成される。ことのき、必要に応じて核酸分離装置１全体を振って検体Ｓと薬液ＬＡとの混合を促進する。この際にも、逆止部１１４０、１１５０、１１６０等の逆止部は、混合された液体Ｌが収容空間１１の側から第１空間１１０１の側に流入することを妨げることができる。 A liquid L is formed by mixing the sample S and the drug solution LA in the storage space 11 of the nucleic acid processing container 10. Kotonoki, waving entire nucleic acid separation device 1 as needed to facilitate mixing of the specimen S and the chemical L A. Also at this time, the non-returning portions 1140, 1150, 1160 and the like can prevent the mixed liquid L from flowing from the side of the accommodating space 11 to the side of the first space 1101.

また、本実施形態では、担体保持部１２０と組み合わせる液体収容容器１０００が、ロック部２０と一体化されたユニット２の形態である。図７Ａ、７Ｂに示すように、ストッパー部材３０を設けることで、押圧部１１２１を介した蛇腹部１１２０への意図しない押圧が制限されるため、ユニット２の形態の液体収容容器１０００の液体収容部１１３０を、担体保持部１２０の薬液供給口周縁部１３１２に押し込んで接続する操作が容易になるため好ましい。 Further, in the present embodiment, the liquid storage container 1000 to be combined with the carrier holding portion 120 is in the form of the unit 2 integrated with the lock portion 20. As shown in FIGS. 7A and 7B, by providing the stopper member 30, unintended pressing on the bellows portion 1120 via the pressing portion 1121 is restricted, so that the liquid accommodating portion of the liquid accommodating container 1000 in the form of the unit 2 is restricted. It is preferable because the operation of pushing the 1130 into the peripheral edge portion 1312 of the chemical solution supply port of the carrier holding portion 120 to facilitate the connection.

続いて、ロック部２０により、蛇腹部１１２０の変形状態を保持する機構について説明する。 Subsequently, a mechanism for holding the deformed state of the bellows portion 1120 by the lock portion 20 will be described.

図７Ａ、７Ｂに示す状態の核酸分離装置１からストッパー部材３０を取り外し、核酸処理容器１０の蛇腹部１１２０を軸Ｘに沿って圧縮変形させると、図７Ｃに示すように、核酸処理容器１０の、液体収容部側係止部（第１係止部）１３１２Ｄと、ロック部２０の、ロック部側係止部（第２係止部）２３０とが互いに係止され、蛇腹部１１２０の形状の初期状態への回復が阻止されるため、蛇腹部１１２０の変形状態が保持される。このとき、逆止部１１４０、１１５０、１１６０等の逆止部は、蛇腹部１１２０内の第１空間１１０１で圧縮された空気の、収容空間１１への流入を許容する。 When the stopper member 30 is removed from the nucleic acid separation device 1 in the state shown in FIGS. 7A and 7B and the bellows portion 1120 of the nucleic acid processing container 10 is compressed and deformed along the axis X, as shown in FIG. 7C, the nucleic acid processing container 10 , The liquid accommodating portion side locking portion (first locking portion) 1312D and the locking portion side locking portion (second locking portion) 230 of the locking portion 20 are locked to each other, and have the shape of the bellows portion 1120. Since the recovery to the initial state is prevented, the deformed state of the bellows portion 1120 is maintained. At this time, the check portions such as the check portions 1140, 1150, and 1160 allow the air compressed in the first space 1101 in the bellows portion 1120 to flow into the accommodation space 11.

図７Ｃは、蛇腹部１１２０を圧縮変形させた直後の、液体Ｌが担体１３を透過して排出される前の状態を示す。この状態のとき収容空間１１内の気圧は最大圧となり、時間経過とともに液体Ｌが担体１３を通過して排出口１２から排出され、液体Ｌの排出に伴い収容空間１１内の気圧は低減し、最終的には、図７Ｄに示すように、液体Ｌが収容空間１１から回収容器４０内に全て排出した状態に至る。 FIG. 7C shows a state immediately after the bellows portion 1120 is compressed and deformed, and before the liquid L is discharged through the carrier 13. In this state, the air pressure in the accommodation space 11 becomes the maximum pressure, the liquid L passes through the carrier 13 and is discharged from the discharge port 12 with the passage of time, and the air pressure in the accommodation space 11 decreases as the liquid L is discharged. Eventually, as shown in FIG. 7D, the liquid L is completely discharged from the storage space 11 into the recovery container 40.

＜核酸分離装置１を用いた核酸含有検体の処理＞
上記の核酸分離装置１を用いて、核酸を含む検体Ｓと、検体Ｓを処理する薬液ＬＡとを混合して得た液体Ｌを、核酸を吸着する又は核酸を通過させる液透過性担体１３に透過して処理する方法について以下に説明する。<Treatment of Nucleic Acid-Containing Specimen Using Nucleic Acid Separator 1>
A liquid-permeable carrier 13 that adsorbs or passes a nucleic acid to a liquid L obtained by mixing a sample S containing a nucleic acid and a drug solution LA for processing the sample S using the above-mentioned nucleic acid separation device 1. The method of transparently processing the nucleic acid will be described below.

本実施形態ではまず、図６Ｂに示すように、担体保持部１２０の検体収容空間１３１に、核酸を含む検体Ｓを収容する。核酸を含む検体Ｓとしては、例えば生体試料、飲食物、河川水、海水等が挙げられる。生体試料はヒト等の動物に由来するものであってもよいし、植物に由来するものであってもよいし、微生物に由来するものであってもよい。生体試料としては血液、尿、糞便、喀痰、唾液、鼻汁、拭い液等が挙げられる。 In the present embodiment, first, as shown in FIG. 6B, the sample S containing nucleic acid is stored in the sample storage space 131 of the carrier holding unit 120. Examples of the sample S containing nucleic acid include biological samples, foods and drinks, river water, seawater and the like. The biological sample may be derived from an animal such as a human, a plant, or a microorganism. Examples of biological samples include blood, urine, feces, sputum, saliva, nasal discharge, and wiping liquid.

続いて、図７Ａ、７Ｂに示すように、ユニット２が備える液体収容容器１０００の液体収容部１１３０と、検体Ｓを収容した担体保持部１２０の薬液供給口周縁部１３１２との接続により、液体収容容器１０００の蓋体１２００を破壊して、液体収容容器１０００の第２空間１１０２と、担体保持部１２０の検体収容空間１３１とを連通し、収容空間１１を内包する核酸処理容器１０を形成する。 Subsequently, as shown in FIGS. 7A and 7B, the liquid storage unit 1130 of the liquid storage container 1000 provided in the unit 2 and the peripheral portion 1312 of the chemical liquid supply port of the carrier holding unit 120 containing the sample S are connected to store the liquid. The lid 1200 of the container 1000 is broken, and the second space 1102 of the liquid storage container 1000 and the sample storage space 131 of the carrier holding portion 120 are communicated with each other to form a nucleic acid processing container 10 containing the storage space 11.

続いて、収容空間１１内で、液体ＬＡと検体Ｓを混合し、検体Ｓから核酸を遊離させて、核酸を含む液体Ｌを生成する。液体ＬＡと検体Ｓとの混合のために、図７Ａ、７Ｂに示す核酸分離装置１の全体を振ることが好ましい。逆止部１１４０、１１５０、１１６０等の逆止部は、混合された液体Ｌが収容空間１１の側から第１空間１１０１の側に流入することを妨げるため、液体Ｌが、蛇腹部１１２０の内壁面１１２０Ｂの複数の谷Ｖに取り込まれることが回避される。Subsequently, in the accommodation space within 11, the liquid L A and the specimen S were mixed and to release nucleic acids from the sample S, to produce a liquid L including a nucleic acid. For mixing with the liquid L A and the specimen S, it is preferable to shake the entire nucleic acid separation apparatus 1 shown in FIG. 7A, 7B. The non-returning portions 1140, 1150, 1160 and the like prevent the mixed liquid L from flowing from the side of the accommodating space 11 to the side of the first space 1101, so that the liquid L is contained in the bellows portion 1120. It is avoided that the wall surface 1120B is taken in by a plurality of valleys V.

続いて、図７Ａ、７Ｂに示す核酸分離装置１からストッパー部材３０を取り外し、核酸処理容器１０の蛇腹部１１２０を軸Ｘに沿って圧縮変形させる。図７Ｃに示すように、核酸処理容器１０の、液体収容部側係止部（第１係止部）１３１２Ｄと、ロック部２０の、ロック部側係止部（第２係止部）２３０とが互いに係止し、蛇腹部１１２０の圧縮変形した状態が保持される。逆止部１１４０、１１５０、１１６０等の逆止部は、蛇腹部１１２０内の第１空間１１０１で圧縮された空気の、収容空間１１への流入を許容する。蛇腹部１１２０の圧縮変形により収容空間１１内の気圧は高まり、液体Lは収容空間１１の高められた気圧により圧送され、担体１３を透過しながら、排出口１２から排出され、回収容器４０内に回収される（図７Ｄ）。この過程で、収容空間１１内の気圧は最大圧に達した後は時間経過とともに徐々に低減する。 Subsequently, the stopper member 30 is removed from the nucleic acid separation device 1 shown in FIGS. 7A and 7B, and the bellows portion 1120 of the nucleic acid processing container 10 is compressed and deformed along the axis X. As shown in FIG. 7C, the liquid storage portion side locking portion (first locking portion) 1312D of the nucleic acid processing container 10 and the locking portion side locking portion (second locking portion) 230 of the lock portion 20. Are locked to each other, and the compressed and deformed state of the bellows portion 1120 is maintained. The check portions such as the check portions 1140, 1150, and 1160 allow the air compressed in the first space 1101 in the bellows portion 1120 to flow into the accommodation space 11. The air pressure in the accommodating space 11 increases due to the compressive deformation of the bellows portion 1120, and the liquid L is pumped by the increased air pressure in the accommodating space 11 and discharged from the discharge port 12 while passing through the carrier 13 and into the collection container 40. It is recovered (Fig. 7D). In this process, the air pressure in the accommodation space 11 gradually decreases with the passage of time after reaching the maximum pressure.

担体１３が、核酸を通過させる液透過性担体である実施形態では、回収容器４０内に回収された液体は、核酸を含んでおり、担体１３により不純物が除去された液体である。 In the embodiment in which the carrier 13 is a liquid-permeable carrier through which nucleic acid is passed, the liquid recovered in the recovery container 40 is a liquid containing nucleic acid and impurities are removed by the carrier 13.

担体１３が、核酸を吸着する液透過性担体である実施形態では、液体Ｌを透過させた後の担体１３は核酸を吸着し担持している。この実施形態では、更に、核酸を担持している担体１３を洗浄液により洗浄して不純物を除去し、その後、担体１３から核酸を分離して回収することが好ましい。そこで、図８〜１２を参照して、核酸を担持している担体１３の洗浄と、担体１３からの核酸の分離回収について説明する。 In the embodiment in which the carrier 13 is a liquid-permeable carrier that adsorbs nucleic acid, the carrier 13 after permeating the liquid L adsorbs and carries the nucleic acid. In this embodiment, it is preferable that the carrier 13 carrying the nucleic acid is further washed with a washing solution to remove impurities, and then the nucleic acid is separated and recovered from the carrier 13. Therefore, the washing of the carrier 13 carrying the nucleic acid and the separation and recovery of the nucleic acid from the carrier 13 will be described with reference to FIGS. 8 to 12.

図８は、図７Ｄに示す液体Ｌ透過後の核酸分離装置１において、担体保持部１２０の第１部材１３１０よりも上流側を取り外し、核酸を担持している担体１３を含む第２部材１３２０と回収容器４０を残した状態を模式的に示す。 FIG. 8 shows, in the nucleic acid separation device 1 after permeation of the liquid L shown in FIG. 7D, the upstream side of the carrier holding portion 120 from the first member 1310 is removed, and the second member 1320 including the carrier 13 carrying the nucleic acid is shown. The state in which the collection container 40 is left is schematically shown.

図９は、核酸を担持している担体１３を洗浄するための洗浄液ＬＢを収容した洗浄液収容容器２０００及びロック部２０を備えたユニット３の断面模式図である。Figure 9 is a cross-sectional schematic view of the unit 3 with a cleaning liquid container 2000 and a lock portion 20 accommodating the cleaning liquid L B for washing the carrier 13 carrying the nucleic acid.

洗浄液収容容器２０００は、内部空間２００１を内包し、内部空間２００１に洗浄液ＬＢが収容されており、内部空間２００１に収容された洗浄液ＬＢを放出する放出口２００２が形成されている洗浄液容器本体２１００と、放出口２００２を封止する蓋体２２００とを備える。Washing liquid container 2000 encloses an interior space 2001, and the cleaning liquid L B is accommodated in the inner space 2001, the washing liquid container outlet 2002 to release the cleaning liquid L B contained in the internal space 2001 is formed body It includes a 2100 and a lid 2200 that seals the discharge port 2002.

洗浄液容器本体２１００は、その一部に、内部空間２００１の体積が減少可能に変形する変形部分である、蛇腹部２００３を備える。蛇腹部２００３は、軸Ｙに沿って圧縮変形可能であり、圧縮変形に伴い内部空間２００１の体積が減少する。 The cleaning liquid container main body 2100 includes a bellows portion 2003, which is a deformed portion in which the volume of the internal space 2001 is deformed so as to be reduced. The bellows portion 2003 can be compressionally deformed along the axis Y, and the volume of the internal space 2001 decreases with the compression deformation.

洗浄液容器本体２１００のうち、放出口２００２の周囲を囲う部分が、洗浄液収容部２９００である。洗浄液収容部２９００は、蛇腹部２００３に接続された筒状の主部２９０１と、主部２９０１の端部から軸Ｙの方向に延在し、放出口２００２を画定する、図５Ｃに示す第２部材１３２０の開口１３２１の内径よりも小さな外径を有する、筒状の第５延在部２９０２と、主部２９０１の端部から軸Ｙの方向に延在する、図５Ｃに示す第２部材１３２０の第３延在部１３２２の外径よりも僅かに大きな内径を有する、筒状の第６延在部２９０３とが形成されている。第６延在部２９０３の内周面２９０３Ａには、第２部材１３２０の第３延在部１３２２の第３螺合部１３２２Ｂと螺合するための第６螺合部２９０３Ｂが設けられている。洗浄液収容部２９００は、その外周面２９００Ａ上に、周方向の全体に亘って延在し、径方向外方に突出した係止爪である、第３係止部２９００Ｂを備える。 The portion of the cleaning liquid container main body 2100 that surrounds the discharge port 2002 is the cleaning liquid accommodating portion 2900. The cleaning liquid accommodating portion 2900 extends from the end of the tubular main portion 2901 connected to the bellows portion 2003 and the end portion of the main portion 2901 in the direction of the axis Y to define the discharge port 2002, the second shown in FIG. 5C. A tubular fifth extending portion 2902 having an outer diameter smaller than the inner diameter of the opening 1321 of the member 1320, and a second member 1320 extending in the direction of the axis Y from the end of the main portion 2901. A tubular sixth extending portion 2903 having an inner diameter slightly larger than the outer diameter of the third extending portion 1322 of the above is formed. The inner peripheral surface 2903A of the sixth extending portion 2903 is provided with a sixth screwing portion 2903B for screwing with the third screwing portion 1322B of the third extending portion 1322 of the second member 1320. The cleaning liquid accommodating portion 2900 includes a third locking portion 2900B which is a locking claw extending outward in the radial direction and extending over the entire circumferential direction on the outer peripheral surface 2900A.

本実施形態に用いる洗浄液ＬＢは、核酸が吸着した担体１３に残存する可溶化試薬や試料由来の夾雑物を洗浄できるものであれば良く、核酸が溶解しない有機溶媒、水溶性高分子溶液、糖水溶液が例示されるがこれに限定されない。洗浄液ＬＢとして用いることができる有機溶媒としてはエタノール、イソプロパノール、アセトンなどが挙げられる。有機溶媒として水溶性有機溶媒を用いる場合、有機溶媒の水溶液を用いることができ、その濃度は当業者によれば容易に最適値を決定することができるが、一例として２０〜１００重量％、好ましくは３０〜９０重量％、より好ましくは４０〜８０重量％が挙げられる。The cleaning liquid L B used in the present embodiment, the nucleic acid as long as it can be cleaned of contaminants from solubilized reagent and sample remaining in the carrier 13 that has adsorbed an organic solvent in which the nucleic acid is not soluble, water-soluble polymer solution, An aqueous sugar solution is exemplified, but the present invention is not limited to this. Examples of the organic solvent can be used as a cleaning liquid L B ethanol, isopropanol, and acetone. When a water-soluble organic solvent is used as the organic solvent, an aqueous solution of the organic solvent can be used, and the optimum concentration thereof can be easily determined by those skilled in the art, but as an example, 20 to 100% by weight is preferable. 30 to 90% by weight, more preferably 40 to 80% by weight.

図１０Ａに示すように、洗浄液収容容器２０００及びロック部２０を備えたユニット３を、核酸を担持している担体１３を含む第２部材１３２０に接続する。この接続は、洗浄液収容容器２０００の洗浄液収容部２９００の第６螺合部２９０３Ｂと、第２部材１３２０の第３延在部１３２２の第３螺合部１３２２Ｂとを螺合することで行う。この接続の際に、第２部材１３２０が備える第４突出部１３２６の先端が、洗浄液収容容器２０００の放出口２００２を封止する蓋体２２００に押し当てられて蓋体２２００を破壊し、洗浄液収容容器２０００の放出口２００２を開放する。 As shown in FIG. 10A, the unit 3 provided with the cleaning liquid storage container 2000 and the lock portion 20 is connected to the second member 1320 including the carrier 13 carrying the nucleic acid. This connection is made by screwing the sixth screwed portion 2903B of the cleaning liquid accommodating portion 2900 of the cleaning liquid accommodating container 2000 and the third screwed portion 1322B of the third extending portion 1322 of the second member 1320. At the time of this connection, the tip of the fourth protruding portion 1326 included in the second member 1320 is pressed against the lid 2200 that seals the discharge port 2002 of the cleaning liquid storage container 2000 to destroy the lid 2200 and store the cleaning liquid. The discharge port 2002 of the container 2000 is opened.

続いて、図１０Ｂに示すように、洗浄液収容容器２０００の蛇腹部２００３を軸Yに沿って圧縮変形させる。このとき、洗浄液収容容器２０００の第３係止部２９００Ｂと、ロック部２０の、ロック部側係止部（第２係止部）２３０とが互いに係止し、蛇腹部２００３の圧縮変形した状態が保持される。蛇腹部２００３の圧縮変形により内部の気圧は高まり、洗浄液ＬＢは高められた気圧により圧送され、核酸を担持する担体１３を透過しながら、排出口１２から排出され、回収容器４０内に回収される。Subsequently, as shown in FIG. 10B, the bellows portion 2003 of the cleaning liquid storage container 2000 is compressed and deformed along the axis Y. At this time, the third locking portion 2900B of the cleaning liquid storage container 2000 and the locking portion side locking portion (second locking portion) 230 of the locking portion 20 are locked to each other, and the bellows portion 2003 is compressed and deformed. Is retained. Increasing the internal pressure by the compression deformation of the bellows portion 2003, the cleaning liquid L B is pumped by pressure elevated, while transmitting a carrier 13 carrying the nucleic acid, is discharged from the discharge port 12, it is recovered in the recovery container 40 To.

核酸を担持した担体１３を洗浄液ＬＢにより洗浄した後、核酸を担持した担体１３を保持する第２部材１３２０を取り出す（図１１）。別途、図１２に示すように、担体１３から核酸を溶出させる溶離液ＬＣを充填したシリンジ５０を用意する。シリンジ５０から溶離液ＬＣを、核酸を担持した担体１３に向けて注入して通過させ、核酸を含む溶離液ＬＣを試料採取容器６０に採取する。After the carrier 13 carrying the nucleic acid was washed with the washing liquid L B, taken out second member 1320 that holds the carrier 13 carrying the nucleic acid (FIG. 11). Separately, as shown in FIG. 12, providing a syringe 50 filled with eluents L C eluting the nucleic acid from the carrier 13. The eluate L C from the syringe 50, the nucleic acid is injected toward the carrier 13 which carries passed through, collecting the eluate L C containing nucleic acid in the sample collection container 60.

本実施形態に用いる溶離液ＬＣは、担体１３に吸着された核酸を溶離させることが可能な液体であれば特に限定されない。溶離液ＬＣとしては水、トリス塩酸緩衝液などの緩衝液等が挙げられる。溶離液ＬＣには溶離液としての機能を阻害しない限り、またＰＣＲ反応を阻害しない限り、ｐＨ緩衝性成分、塩等の成分が含まれていてもよい。Eluent L C used in the present embodiment is not particularly limited as long as it is liquid capable of eluting the nucleic acid adsorbed to the carrier 13. The eluent L C water, a buffer solution such as Tris-HCl buffer and the like. Unless inhibit the function of the eluent in the eluent L C, also as long as it does not inhibit the PCR reaction, pH buffering component, may contain components such as salt.

本明細書で引用した全ての刊行物、特許及び特許出願はそのまま引用により本明細書に組み入れられるものとする。 All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.

Claims (9)

液体を収容し放出する液体収容容器であって、
第１空間を内包し、前記第１空間の体積が減少するように１つの軸に沿って圧縮変形可能な蛇腹部と、
前記蛇腹部の一端に接続され、液体を収容するための第２空間を内包し、液体を放出するための放出口が形成された液体収容部と、
前記第２空間から前記第１空間への液体の流入を妨げ、且つ、前記蛇腹部を圧縮変形させたときの前記第１空間から前記第２空間への気体の流入を許容する逆止部と
を含む容器本体
を備える液体収容容器。A liquid container that stores and discharges liquids.
A bellows portion that includes the first space and can be compressed and deformed along one axis so that the volume of the first space is reduced.
A liquid accommodating portion connected to one end of the bellows portion, containing a second space for accommodating the liquid, and forming a discharge port for discharging the liquid.
A check valve that prevents the inflow of liquid from the second space into the first space and allows the inflow of gas from the first space to the second space when the bellows portion is compressed and deformed. A liquid storage container comprising a container body containing. 前記逆止部が、前記第１空間と前記第２空間とを接続する貫通孔を有する、請求項１に記載の液体収容容器。 The liquid storage container according to claim 1, wherein the check valve has a through hole connecting the first space and the second space. 前記貫通孔の最狭部の幅が０．５ｍｍ以下である、請求項２に記載の液体収容容器。 The liquid storage container according to claim 2, wherein the width of the narrowest portion of the through hole is 0.5 mm or less. 前記貫通孔の長さが０．１ｍｍ以上である、請求項２又は３に記載の液体収容容器。 The liquid storage container according to claim 2 or 3, wherein the length of the through hole is 0.1 mm or more. 前記貫通孔を閉塞し、前記蛇腹部を圧縮変形させたときに前記貫通孔を開放する閉塞部を更に含む、請求項２〜４のいずれか１項に記載の液体収容容器。 The liquid storage container according to any one of claims 2 to 4, further comprising a closed portion that closes the through hole and opens the through hole when the bellows portion is compressively deformed. 前記逆止部が多孔質体を含む、請求項１に記載の液体収容容器。 The liquid storage container according to claim 1, wherein the check valve contains a porous body. 前記逆止部が逆止弁である、請求項１に記載の液体収容容器。 The liquid storage container according to claim 1, wherein the check valve is a check valve. 前記第２空間に収容された液体を更に備え、
前記液体が、核酸、核酸を含有する検体、又は核酸を担持した担体を処理するための薬液である、請求項１〜７のいずれか１項に記載の液体収容容器。Further provided with the liquid contained in the second space,
The liquid storage container according to any one of claims 1 to 7, wherein the liquid is a nucleic acid, a sample containing nucleic acid, or a chemical solution for treating a carrier carrying nucleic acid. 前記放出口を封止する蓋体を更に備える、請求項８に記載の液体収容容器と、
核酸を含む検体を収容するための検体収容空間を内包し、前記検体収容空間の一方側に、前記薬液が供給可能なように前記液体収容容器の前記放出口に接続される薬液供給口が形成されており、前記検体収容空間の他方側に、前記薬液と前記検体との混合物を、前記混合物中の核酸を吸着する又は核酸を通過させる液透過性担体を介して排出する排出口が形成されている担体保持部と、
を備え、
前記担体保持部は、前記薬液供給口に前記液体収容容器を接続する際に、前記液体収容容器の、前記蓋体による前記放出口の封止を開放し、前記放出口と前記薬液供給口とを連通する、開放部を備える、核酸分離装置。The liquid storage container according to claim 8, further comprising a lid for sealing the discharge port.
A sample storage space for storing a sample containing nucleic acid is included, and a drug solution supply port connected to the discharge port of the liquid storage container is formed on one side of the sample storage space so that the drug solution can be supplied. On the other side of the sample storage space, a discharge port is formed for discharging the mixture of the drug solution and the sample via a liquid-permeable carrier that adsorbs the nucleic acid in the mixture or allows the nucleic acid to pass through. Carrier holder and
With
When the liquid storage container is connected to the chemical solution supply port, the carrier holding portion opens the seal of the discharge port of the liquid storage container by the lid body, and the discharge port and the chemical solution supply port A nucleic acid separator having an open portion for communicating with each other.
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