JPWO2017175833A1 - Apparatus for producing ethanol from biomass resources and method for producing ethanol from biomass resources - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a technology capable of carrying out a distillation process without elevating the concentration of ethanol in a culture medium during saccharification fermentation and transferring impurities such as raw materials and cells to a distillation apparatus.
An ethanol production apparatus according to the present invention comprises: a fermentation culture tank for saccharifying and fermenting biomass resources; a supply port capable of supplying gas to the fermentation culture tank; a recovery pipe for liquefying the gas discharged from the fermentation culture tank And a recovery tank for recovering ethanol discharged from the recovery pipe. A fermentation and culture process for saccharifying and fermenting biomass resources using the ethanol production apparatus of the present invention, a distillation process for distilling a gas discharged from the fermentation culture tank, and a recovery process for recovering the gas discharged from the exhaust process. carry out.
[Selected figure] Figure 1

Description

The present invention relates to an apparatus for producing ethanol from biomass resources and a method for producing ethanol from biomass resources.

BACKGROUND ART In recent years, bioethanol that produces ethanol by fermentation of microorganisms from grains or the like has attracted attention as an effort to realize suppression of the amount of fossil fuel used. Various processes have been studied and presented as production techniques.

However, at present, production of bioethanol etc. from food grains (eg, corn, sugar cane etc.) is being carried out, but this has led to soaring food prices, which is a distant cause of making economic activities unstable. There is. Furthermore, they are conflicting and social problems because they are human food. Therefore, ethanol production from cellulose-containing biomass (for example, herbaceous biomass such as rice straw and straw, woody biomass such as trees and waste building materials) and industrial waste has become an important technology development.

In the method of producing ethanol by saccharifying and fermenting a raw material suspension containing biomass raw material as described in Document 1 below, in the ethanol separation step, ethanol can be separated from the liquid separated in the solid-liquid separation step which is the previous step It is disclosed.

JP 2015-27285

However, in the method described in Patent Document 1, it is necessary to take out the culture solution after completion of the culture, perform solid-liquid separation, and then perform the distillation step. This is because the ethanol fermentation step is performed under anaerobic conditions and at a temperature of 30 ° C. or less, and thus the produced ethanol is still present in the medium after the completion of the culture. In such a fermentation process, as the fermentation proceeds and the ethanol concentration in the culture medium increases, the product inhibition to the fermentation microorganism is expanded and the ethanol production by the fermentation microorganism is stopped. In addition, there is a problem that fermented microorganisms are killed by the produced ethanol. Furthermore, there is a problem that the fermentation microorganism is killed during the distillation process.

Therefore, in the present invention, the distillation step is carried out without elevating the concentration of ethanol in the culture medium during saccharification fermentation, without transferring impurities such as raw materials and cells to the distillation apparatus and without killing the cells. Aims to provide technology that can

The inventor of the present invention focused on the feature that, when alcohol fermentation is performed using a microorganism having an ethanol producing ability that can be cultured under aerobic conditions, a part of generated ethanol is volatilized. By ventilating the volatilized ethanol into the fermentation culture solution, the water vapor containing ethanol is discharged out of the fermentation culture tank system, and dissolved in the solution in the recovery tank provided outside the fermentation culture tank, the raw materials and It was found that a solution containing ethanol free of impurities such as cells was obtained.

According to one aspect of the present invention, an ethanol production apparatus includes: a fermentation culture tank that performs saccharification and fermentation of a biomass resource; a supply port that can supply a gas to the fermentation culture tank; The gist of the invention is to have a recovery pipe for recovering the gas, and a recovery tank for recovering the ethanol discharged from the recovery pipe.

Moreover, according to one aspect of the present invention, the method for producing ethanol comprises: a fermentation culture step of saccharifying and fermenting a biomass resource; a distillation step of distilling a gas discharged from the fermentation culture tank; The gist of the present invention is to have a recovery step of recovering a gas.

According to the present invention, the produced ethanol can be recovered outside the fermentation culture tank system without expanding the product inhibition to the fermentation microorganism by the produced ethanol.

General schematic according to the present invention It is a top view of a fermentation culture tank. FIG. 1 is a plan view of an apparatus for producing ethanol according to the present invention. It is a front view of the apparatus which manufactures the ethanol which concerns on this invention. FIG. 1 is a right side view of an apparatus for producing ethanol according to the present invention. 1 is an aspect of a recovery pipe according to the present invention. 1 is an aspect of a recovery pipe according to the present invention. It is one aspect of the collection tank used for this invention, and a collection tank. It is process drawing concerning the present invention. 7 shows the results for Example 1. 7 shows the results for Example 2. 7 shows the results for Example 3. 7 shows the results for Example 3. 7 shows the results for Example 4. 7 shows the results for Example 5. 7 shows the results for Example 6. 7 shows the results for Example 7. 7 shows the results for Example 7.

The present invention is a method for carrying out a distillation step without increasing the concentration of ethanol in the culture medium during saccharification fermentation, and transferring the impurities to a distillation apparatus, and an apparatus for realizing the method.
The invention will now be described in more detail with reference to the accompanying drawings. Note that the descriptions of materials, methods, numerical ranges and the like described in the present specification are not intended to limit the present materials, methods, numerical ranges, and the like, and other materials, methods, and numerical ranges may be used. It does not exclude the use such as.

As shown in FIG. 1, an apparatus A for producing ethanol from biomass resources according to the present embodiment includes a fermentation culture tank 1 and a recovery pipe 2 for transferring ethanol volatilized in the fermentation culture tank, and a recovery pipe 2 And a distiller 4 for distilling an ethanol solution in which ethanol is dissolved in the solution in the recovery tank 3.
In addition, it is also possible to use a plurality of recovery tanks 3 and connect them linearly or radially using the above recovery tubes (not shown). By doing so, the amount of ethanol to be recovered can be increased. In addition, if necessary, a concentration column (not shown) for concentrating ethanol may be installed in the distiller 4 and an apparatus for performing a dehydration membrane process for dehydration of ethanol may be installed.

The top surface structure of the fermentation culture tank 1 will be described with reference to FIG. The top surface of the fermentation culture vessel is provided with a sample inlet 5 for feeding a material as a substrate, an air supply port 6, a fermentation steam outlet 7 and a stirrer installation port 8. The sample inlet 5 is provided with an openable lid (not shown) having a check window (not shown). In addition, an apparatus for continuously and quantitatively supplying a substrate can be installed in the sample inlet 5 in the previous period (not shown). An air supply pipe (not shown, hereinafter referred to as an air supply pipe) is connected to the air supply port 6 via an air pump (not shown). If the air supply port of the air supply pipe is below the surface of the fermentation culture in the fermentation culture tank 1, the length of the air supply pipe is not particularly limited, but it is preferable to be closer to the bottom. The fermentation and the outlet 5 are connected to the recovery pipe 2. At the stirrer installation port 8, a stirring blade 9 is installed together with a pump (not shown) for a stirrer.
Here, various air pumps commercially available can be used as the air pump, and the air pump is not particularly limited as long as it can supply air. Moreover, the stirring blade 9 can use various commercially available stirring blades.

The structure of the fermentation culture tank 1 is demonstrated using FIGS. 3-5.
The connection port 11 provided on the upper side surface of the fermentation culture tank 1 and the bottom 12 of the fermentation culture tank 1 are connected via a pump 13 and a cyclone cleaner 19 using a circulation pipe 14. Further, the pump 13 and the circulation pipe 14 are connected via the outlet 15.
Fermentation broth is circulated by using pump 13 as circulation means, and the culture broth is separated into solid solution and solution such as culture broth using cyclone cleaner 19 installed in the middle of circulation pipe 14 Do. The solid residue is discharged to the sludge pot 20 through a pipe connected to the cyclone cleaner 19. On the other hand, a solution such as a culture solution is returned to the fermentation culture tank 1 from the connection port 11. In addition to the cyclone cleaner 19, a slit screen or the like may be installed to recover filamentous fungi and the like. In addition, if it is an apparatus which can carry out solid-liquid separation on circulation piping 14 as solid-liquid separation means, the apparatus used for the solid-liquid separation means is not particularly limited. Furthermore, it is possible to use an apparatus which can perform solid-liquid separation via a pump after being collected once in a tank or the like, instead of on the circulation pipe 14.

A heat retention jacket 9 is provided on the lower side surface of the fermentation culture vessel 1 to cover the entire lower side surface. A solvent having a desired temperature is passed through the heat retention jacket 10 to adjust the temperature in the fermentation culture vessel. This temperature may be any temperature at which the microorganism can grow and be fermented by ethanol. The higher the temperature in the fermentation culture tank, the higher the evaporation separation efficiency of ethanol.
Further, a pipe 17 provided with a full conical nozzle 16 at its tip is installed toward the inside of the fermentation culture tank 1 from the connection port 11. At this time, the culture medium in the form of mist can be sprayed and sprayed from the circulating culture medium filling cone nozzle 16 to defoam. This makes it possible to carry out ethanol fermentation continuously. The length of the pipe 17 is approximately equal to the radius of the fermentation culture body. Further, the sample outlet 15 can also be used as a connection unit for sending air or water. Therefore, water can be supplied from the sample outlet 15, and misty water can be supplied to the inside of the fermentation culture tank from the full cone nozzle 16 installed approximately at the center of the inside of the fermentation culture tank 1. The spray shape of the full cone nozzle 16 is not limited to the full cone, as long as bubbles in the fermentation culture tank can be efficiently defoamed.

One aspect of the collection bag 2 is shown in FIG. It comprises a substantially semicircular arc-shaped pipe 24 connected to the reducer 23, a cooling pipe 25 provided with a jacket for letting the cooling water flow, and a pipe 28 (not shown). In the fermentation culture vessel 1, the gas containing the discharged ethanol is liquefied in the cooling pipe 25. The cooling pipe 25 can be cooled by various cooling means, and it is not necessary to cool it.

One aspect of the collection bag 2 is shown in FIG. It comprises a cooling pipe 26 provided with a jacket for passing cooling water connected to the reducer 23, a substantially semicircular arc pipe 27 and a pipe 28 (not shown). In the aspect of the present collection pipe 2, the cooling efficiency of the ethanol can be improved by cooling the water / ethanol mixed solution in the cooling pipe 26 and returning the water to the fermentation culture tank. The cooling pipe 26 can be cooled by various cooling means, and it is not necessary to cool it.

The positional relationship between the recovery tank 3 and the pipe 28 is shown in FIG. The tip of the pipe 28 connected to the recovery tank 2 is near the lower portion of the recovery tank 3. The position of the end of the pipe 28 is not particularly limited, but the end of the pipe 28 may be at a height to be immersed in the solvent introduced into the recovery tank 3. The solvent used in the ethanol production apparatus of the present embodiment may be any solvent that dissolves ethanol, but is preferably water. Furthermore, the recovery method of ethanol is not limited to this method, and may be a method of charging to a tank of distillation equipment such as a general-purpose distillation column.

Next, an ethanol production method using the ethanol production apparatus A will be described with reference to FIG. The method for producing ethanol from biomass resources according to the present invention comprises Step 1 to Step 4 or Step 1 to Step 5.

Step 1 is a fermentation culture process. That is, this is a step of supplying a biomass resource containing lignocellulose such as paper sludge to the fermentation culture tank 2, saccharifying it, and fermenting it. Moreover, the raw material used by this technique is not limited to the said biomass resource, The molasses etc. which are obtained from corn, sugar cane etc. can also be used. In addition, the fermentation culture process which concerns on this embodiment can employ | adopt simultaneous saccharification fermentation process (SSF) or saccharification fermentation simultaneous process (CBP). Thereafter, the microorganism and ethanol production medium are supplied, and cultured at a temperature of 28 ° C. or more, more preferably 40 ° C. or more. By setting the fermentation broth to 40 ° C. or higher, the amount of ethanol present as a gas in the fermentation broth increases.

Prior to the fermentation culture step, a pre-culture step can be carried out. The pre-culture step is a step of adding bacteria to the medium to increase the number of bacteria prior to the fermentation culture step. In addition, as necessary, medium may be added stepwise and / or continuously to increase the medium solution. By carrying out this process, the production rate of ethanol can be improved.

In addition, the culture solution to be introduced into the fermentation culture tank can be prepared, if necessary, to prepare the amount of inoculation (the amount of cells charged). By doing this, it is possible to adjust the amount of ethanol production, the rate of ethanol production, and the cost of ethanol production.

Moreover, solid-liquid separation of the fermentation culture solution is performed in the fermentation culture step. This is done as follows. A fermentation broth discharged from the bottom 12 of the fermentation culture vessel 1 is transferred to a cyclone cleaner 19 through a circulation pipe 14 using a pump 13. Next, solid-liquid separation is performed in the cyclone cleaner 19, and the solid residue during fermentation culture is discharged to the sludge pot 20. On the other hand, the liquid in the fermentation broth is transferred to the connection port 11 via the circulation pipe 14 and returned to the fermentation broth 1. In addition, solid-liquid separation of the fermentation broth can be performed at any timing.

The microorganism having the ability to produce ethanol used in the present invention is a heat-resistant ethanol-fermenting filamentous fungus such as mildew and mushroom. Specific examples thereof include Mucor, Rhizomucor and Rhizopus of the zygomycota, Sizofilm of the basidiomycota (Scizophilum), imperfect bacteria and the like.
Microorganisms that can be used in the simultaneous saccharification and fermentation process (SSF) include the following microorganisms.
Mucor ambiguous, Mucor circinelloides, Mucor fragilis, Mucor hiemalis, Mucor inaequisporus, Mucor oblongiellipticus, Mucor racemosus, Mucor recurves, Mucor satuminus, Mucor subtilissmus, Rhizomucor pusillus, Rhizopus azygosporus, Rhizopus microspores, Rhizopus oryzae, Rhizopus stolonifera, Saccharomyces cerevisiae, Kluyveromyces marxianus , Schizophyllum commune, Fusarium oryzae, Trichoderma ressei
Among these microorganisms, those currently deposited are as follows and can be used in the present invention.
Mucor ambiguous NBRC 6742
Mucor circielloides NBRC 4554, 4569, 4570, 4574, 5382, 5774, 5775, 30470, 31398, 4563, 5398, 6746
Mucor fragilis NBRC 6449, 9402
Mucor hiemalis NBRC 5303, 5834, 8448, 8449, 8565, 8567, 6753, 6754
Mucor inaequisporus NBRC 8624, 8635, 8636
Mucor oblongiellipticus NBRC 9258
Mucor racemosus NBRC 4555, 4556, 4581, 5403, 6745, 9255
Rhizopus azygosporus NBRC 31989
Rhizopus microspores NBRC 4737, 4768, 30499, 31988, 32995, 32996, 8631, 31987, 32002, 32003, 32997
Rhizopus oryzae NBRC 4705, 4706, 4707, 4744, 4758, 4766, 4772, 4780, 4785, 4798, 4804, 4809, 5318, 5319, 5378, 5379, 5413, 5414, 5418, 5440, 5780, 5780 , 5781, 6154, 6155, 6300, 9364, 30795, 31005
Rhizopus stlonifera NBRC 32998, 4781, 5411, 6188, 30816
As these microorganisms, any strain such as a wild strain, a mutant strain, or a recombinant strain derived from genetic techniques such as cell fusion or genetic engineering can be suitably used. Furthermore, these microorganisms can be used alone or in combination. The saccharifying enzymes such as cellulase used simultaneously are not particularly limited, and commercially available saccharifying enzymes such as cellulase agents can be used. In addition, it is possible to mix and use saccharifying enzymes as appropriate.
For example, Amylase, α-Amylase, Glucamylase, Maltase, isoamylase, Cellulase, β-Glucosidase, endo-β-glucanase, Cellobiohydlase, Cellobiase, Xylanase, β-Xylanase, β-1,4-Xylanase, Invertase, Invertase, α-Galacosidase α-Mannosylase, β-Mannosylase, etc. may be mentioned.

The following microorganisms can be mentioned as a thermostable ethanol fermented filamentous fungus which can be used in the saccharification and fermentation simultaneous process (CBP).
Mucor circinelloides, Rhizomucor pusillus, Rhizopus microspores, Schizophyllum commune
Among these microorganisms, those currently deposited are as follows and can be used in the present invention.
Mucor circinelloides NBRC 4563, 5382
Rizomucor pusillus NBRC 4580
Rhizopus microspores NBRC 32997
Schizophyllum commune NBRC 4928, 30749
As these microorganisms, any strain such as a wild strain, a mutant strain, or a recombinant strain derived from genetic techniques such as cell fusion or genetic engineering can be suitably used. Moreover, these microorganisms can be used individually or in mixture. These microorganisms can also secrete and produce saccharifying enzymes such as cellulase.

In addition, the biomass resources include wood, paper, waste paper, fiber, wood pulp, non-wood pulp, paper sludge, rice straw containing cellulose, rice husk, weeds, fallen leaves, seaweed, tea pot, food residue such as coffee ground, papermaking Industrial waste such as sludge, waste containing cellulose components, etc. may be used. Also, of course, anything containing glucose may be used, and it may be generally used corn, sugar cane and other molasses. Furthermore, these residues may be used. In the case of using a microorganism capable of fermenting organic substances such as cellulose, hemicellulose, lignin, various proteins, chitin and the like without being limited to sugars, these can also be used as raw materials.
In addition, paper sludge in which the content of various inorganic components such as calcium carbonate is reduced by treatment such as water washing method, acid treatment method, alkali method, alkali / acid treatment method can also be used. By performing such treatment, the sugar content in paper sludge can be improved, and inorganic components and the like which are fermentation inhibitory substances can be removed to contribute to efficient ethanol fermentation.

As the ethanol production medium, a liquid medium containing a nitrogen source, inorganic salts and, if necessary, organic trace nutrients such as amino acids and vitamins as appropriate, in addition to the aqueous sugar solution, is preferably used. Nitrogen sources include ammonia gas, ammonia water, ammonium salts, urea, nitrates, and other organic nitrogen sources that are used supplementary, such as oil syrups, soybean hydrolyzate, casein hydrolysates, other amino acids, vitamins, Waste molasses, yeast or yeast extract, peptides such as meat extract, peptone, various fermented cells and hydrolysates thereof are used. As the inorganic salts, phosphates, magnesium salts, calcium salts, iron salts, manganese salts and the like can be added as appropriate. It is not limited to the above, and any one that can maintain and improve the viability and productivity of microorganisms can be used.

Step 2 is an insufflation process. The air supply step is a step of feeding air into the fermentation culture tank using an air pump or the like. In the present invention, such a process can be used to perform ethanol fermentation under aerobic conditions. By this process, the ethanol produced and volatilized in the step 1 can be discharged out of the fermentation culture tank system. In addition, it is possible to prevent the ethanol concentration in the culture solution from rising to stop the production of ethanol by the microorganism and to prevent the microorganism from being killed.

Step 3 is a distillation first step. That is, this is a step of liquefying ethanol contained in the gas discharged from the fermentation culture tank using the recovery pipe 2. By this step, ethanol generated in the fermentation culture tank can be recovered outside the fermentation culture tank system. Generally, in the case of distillation after recovery from a fermenter to a distillation tank, yeast and microorganisms are killed and killed by the heat at the time of distillation, but in the method described above, vaporization directly from the fermentation culture tank at a temperature of 50 ° C. or less. Therefore, the microorganism can be repeatedly used without killing it.

Step 4 is a recovery step. That is, this is a step of dissolving the ethanol obtained in step 3 in the solvent prepared in the recovery tank. Moreover, since this process is a process aiming at dissolving ethanol in a solvent, the number of recovery tanks to be used is not particularly limited. By this step, ethanol produced in the fermentation culture tank can be recovered outside the system as an ethanol solution. In addition, by using a plurality of recovery tanks, the amount of recovered ethanol can be increased. Alternatively, it may be directly recovered to the tank of the second distillation step of step 5.

Step 5 is a distillation second step. That is, the ethanol solution obtained in step 4 is finally distilled. Ethanol can be obtained through such a process. In the present embodiment, a method by distillation is adopted, but instead of distillation, solvent extraction method, separation membrane or the like may be separated. It is understood that the step for further concentrating the obtained ethanol is also included in the second distillation step.

Also, the steps 1 to 4 can be performed continuously. That is, the ethanol solution can be continuously produced by continuously feeding the biomass resources and the culture solution which are raw materials additionally and continuously performing the fermentation culture step, the air feeding step, the first distillation step and the recovery step. it can. Furthermore, it is also possible to carry out the steps 1 to 5 continuously. That is, continuous operation of ethanol fermentation becomes possible by continuously performing the fermentation culture step, the air feeding step, the first distillation step, the recovery step, and the second distillation step. In addition, the residual water remaining in the second distillation step can be reused in alkali treatment or acid treatment of paper sludge, or in a fermentation culture step.

EXAMPLES The present invention will next be described in more detail by way of examples, which should not be construed as limiting the invention thereto.

Example 1
(Measurement of ethanol concentration in fermentation culture tank)
Distilled water containing 50 g / L ethanol was used as a model culture, ethanol was evaporated from a 1 L ventilated stirred bioreactor, and changes in the ethanol concentration in the culture solution under various conditions were investigated. The ethanol concentration in the culture solution was sampled at a predetermined time and quantified using high performance liquid chromatography (Shimadzu LC-10 System) according to the following measurement method (in the present invention, the measurement of ethanol is based on the measurement method) Carried out.)
Evaporation experiments were carried out at culture temperatures of 28, 40, 45 and 50 ° C under conditions of aeration air volume of 0.2 VVM.

Measurement method of ethanol concentration (50g / L or more) Separation column: ICSepe WA-1 Wine Analysis (Transgenomic)
Mobile phase: 0.0025N H2SO4
Temperature: 40 ° C
Flow rate: 0.6 mL / min
Detector: Differential Refraction Detector (RID)
For quantification of ethanol, a calibration curve prepared from the results obtained for samples (N サ ン プ ル 5) appropriately diluted with a standard aqueous ethanol solution in advance was used.

The results are shown in FIG. As apparent from FIG. 10, the ethanol concentration in the culture solution is reduced under any conditions, and ethanol is evaporated from the culture solution. It was found that about 40% of ethanol in the bioreactor can be evaporated and recovered in 72 hours of culture at a culture temperature of 40.degree. In addition, it was found that the evaporation rate of ethanol also increases as the culture temperature rises.

Example 2
(Measurement of ethanol concentration in recovery tank)
In the same manner as in Example 1, distilled water containing 50 g / L ethanol was used as a model culture, ethanol was evaporated from a 1 L aeration-agitation bioreactor, and the ethanol concentration change in the recovery tank under various conditions was investigated. The amount of aeration air was 0.2 VVM, and the evaporation test was conducted at a culture temperature of 28, 40, 45 and 50 ° C., and the concentration of the ethanol solution recovered by evaporation was measured at appropriate times.

The results are shown in FIG. At 28 ° C., about 35 g / L of ethanol could be recovered from 12 hours of culture. On the other hand, under conditions of 40 ° C. or higher, the concentration of the aqueous ethanol solution which evaporated even after 6 hours increased, and the aqueous ethanol solution of 200 to 330 g / L could be recovered.

In general, the evaporation of ethanol in a closed vessel depends on the "vapor pressure ratio" of water and ethanol. This ratio is not so dependent on temperature and is about 2.4. Under these conditions, it can be assumed that the ethanol concentration on the gas phase side from the 50 g / L aqueous ethanol solution is at most 120 g / L. However, it has been proved that if aeration is performed from the lower part of the apparatus, it is possible to separate and collect more ethanol. From this result, it has many advantages such as reduction of energy consumption of evaporation process, ease of operation of the apparatus itself since only ethanol steam enters the recovery tank, and avoidance of ethanol inhibition of heat-resistant ethanol filamentous fungus. New ethanol production process.
[Example 3]

(Experiment using reaction culture tank)
A culture experiment using α-cellulose as a model substrate was performed. In this example, 10 L of water was put in a 20 L tank as a recovery tank, and the air exhausted directly from the culture tank was bubbled there. The culture was performed at 40 ° C. using a heat-resistant ethanol filamentous fungus with an aeration amount of 0.2 VVM, and ethanol in the recovery solution was measured every 24 hours. In addition, the contents of glucose and ethanol in the fermentation culture vessel were measured at equal intervals.

FIG. 12 shows the result of the concentration of ethanol in the recovered solution, and FIG. 13 shows the result of measurement of glucose and glucose in the fermentation culture tank. After the start of culture, ethanol by filamentous fungi began to be produced, and the concentration of ethanol in the recovery tank increased accordingly. At 48 hours of culture, ethanol in the culture tank reached 12 g / L. On the other hand, the ethanol concentration in the recovery tank was 36.2 g / L. Therefore, it was proved that ethanol can be concentrated and recovered in the recovery tank.

In culture | cultivation experiment which concerns on a present Example, although it culture | cultivated by making the amount of aeration air into 0.2 VVM, it turned out that the optimization of the amount of aeration gas is necessary from the ethanol still remaining in a culture solution.

Example 4
(Influence of added amount of yeast extract on ethanol fermentation)
Culturing was performed for 72 hours under the condition of 40 ° C. with 100 g / L of paper sludge using a heat-resistant ethanol-fermenting filamentous fungus.

The results are shown in FIG. Under the conditions containing 5 g / L of ordinary yeast extract, 27.4 g / L of ethanol could be produced. On the other hand, when the addition amount was reduced, it slightly decreased at 26.4 g / L at 2 g / L and 24.3 g / L at 1 g / L. Furthermore, it was found that the strain hardly grown and dropped to 4.6 g / L without yeast extract addition. From this result, it was found that yeast extract is essential for growth of thermotolerant ethanol-fermenting filamentous fungi and ethanol fermentation. In addition, although it is desirable that the addition amount be 5 g / L or more, it was found that even at 1 g / L, about 90% of the maximum production capacity can be produced.

[Example 5]
(Influence to ethanol fermentation on addition amount of waste molasses as substitution of yeast extract)
It is considered economically best to use waste molasses (such as corn steep liquor), which is an inexpensive natural medium component, to improve ethanol productivity. Therefore, waste molasses was used as a nitrogen source instead of the yeast extract used for the medium component, and the culture was performed under the same conditions as Example 4 as other conditions.

The results are shown in FIG. When yeast extract is added, ethanol of 20 g / L or more can be produced by addition amount of 1 g / L or more, but about 25 g / L of ethanol can be produced by addition of 2 g / L of waste molasses found.

[Example 6]
(Ethanol production using pretreated PS)
A substrate in which PS treated with CO ₂ for 1 hour or more and subjected to acid treatment (hereinafter referred to as treated PS) and PS not subjected to acid treatment (hereinafter referred to as untreated PS) are used as controls. Then, ethanol was produced in the simultaneous saccharification and fermentation process. The amount of ethanol was measured by carrying out ethanol production for 120 hours with an inoculation amount of 1.25 mL, a culture deposit of 25 L, a culture temperature of 40 ° C. and an aeration amount of 0.1 vvm.

The ethanol measurement result is shown in FIG. The amount of ethanol produced after 120 hours for untreated PS and treated PS was 13.5 g / L and 26.5 g / L, respectively. This result proved that it is possible to produce ethanol using PS as a substrate. In addition, it was also proved that using acid-treated PS as a substrate increases the amount of ethanol produced.

[Example 7]
(Continuous ethanol production using paper pulp)
The ethanol manufacturing apparatus which installed the distiller in the 100 L fermentation tank was prepared, and the culture solution which implemented the preculture process for 24 hours was thrown into the 100 L fermentation tank. Next, after performing batch culture process for 24 hours with 2.5 L of inoculation amount, culture volume of 50 L, culture temperature of 40 ° C. and aeration air volume of 0.1 vvm in a 100 L fermentation tank, continuous culture process The dilution rate of the substrate is 0.04 h ^-1 , the feed rate of the substrate is 0.20 wet-kg / h, and the culture fluid feed rate is 1.8 to 2.0 L / h for 120 hours, and the ethanol in the fermentation culture tank and the recovery tank The amount of ethanol recovered was measured.

The measurement result of ethanol in the distillation tank is shown in FIG. 17, and the integrated amount of recovered ethanol is shown in FIG.
According to Table 8 and Table 9, it was in a steady state in culture | cultivation time 72 hours-144 hours. In addition, it was possible to produce 2.68 kg of ethanol from 14.4 wet-kg of input paper pulp.

1: Fermentation culture tank 2: Recovery pipe 3: Recovery tank 4: Distillation device 5: Sample inlet 6: 6: Air supply port 7: Fermentation steam port 8: Stirrer installation port 9: Stirrer 10: Heat insulation jacket 11: Connection port 12 : Bottom part 13: Pump 14: Circulation piping 15: Sample taking out port 16: Fully conical nozzle 17: Piping 18: Leg 19: Cyclone cleaner 20: Sludge pot 23: Reducer 24: Almost semi-circular piping 25: Cooling pipe 26: Cooling Pipe 27: substantially semicircular arc shaped pipe 28: tip of recovery pipe 2
A: Ethanol production apparatus B: Culture solution C: Recovery solution D: Water vapor containing ethanol E: Ethanol F: Air

Claims (8)

An apparatus for producing ethanol from biomass resources,
A fermentation culture tank for saccharifying and fermenting the biomass resources;
An air supply pipe for supplying a gas to the fermentation culture tank;
A recovery pipe for recovering the gas discharged from the fermentation culture tank;
A recovery tank for recovering ethanol discharged from the recovery pipe;
An apparatus for producing ethanol from biomass resources, comprising: Circulation means for circulating the fermentation culture solution between the bottom and the upper side of the fermentation culture vessel;
Solid content separation means for solid-liquid separation of the fermentation broth in the middle of the circulation means;
The apparatus for producing ethanol from biomass resources according to claim 1, comprising: An apparatus for producing ethanol from biomass resources according to claim 1 or 2
An apparatus for producing ethanol from biomass resources, characterized by using a thermostable ethanol-fermenting filamentous fungus. A method of producing ethanol from biomass resources, comprising
A fermentation culture step of saccharifying and fermenting the biomass resources;
An air supplying step of supplying a gas to the fermentation culture tank;
A distillation step of distilling the gas discharged from the fermentation culture tank;
A recovery step of recovering ethanol discharged from the distillation step;
A method of producing ethanol from biomass resources, characterized in that it comprises: A method of producing ethanol from biomass resources, comprising performing solid-liquid separation of the fermentation culture solution in the fermentation culture step. The method for producing ethanol from biomass resources according to claim 4 or 5, wherein a thermostable ethanol-fermenting filamentous fungus is used in the fermentation culture step. The method for producing ethanol from biomass resources according to any one of claims 4 to 6, wherein the culture medium in the fermentation culture step is a culture medium containing waste molasses as a nitrogen source. The method for producing ethanol from biomass resources according to any one of claims 4 to 7, wherein a pre-culture step is performed prior to the fermentation and culture step.

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